ICSI Cheeta

First Joint TAG Chairs Meeting
Alphen, The Netherlands
1 June 2014
Cooperative Species Management Committee
53 Institutions members of ALPZA
Cooperative Species Management Committee
Coordinator: Adrián Sestelo
Co-coordinator: Guillermo Cubillos
Members: Miguel Bueno – Myriam Salazar – SB Coordinators
18 Regional Studbook
 Humboldt Penguin (Spheniscus humboldti) Management
Plan under the MoU with EAZA
Among the advances worth noting the successful establishment of
colony formed in the La Aurora Zoo, Guatemala (18 individuals).
Regarding the specimens arrived at Metropolitan Park Zoo,
Santiago de Chile, they are in good physical condition and since mid2013 have already started to couples, some reproductive
established.
It argues that by 2014, more institutions join this initiative to
establish different reproductive nuclei in different zoos in Latin
America.
 Towards new regional management plans
Technical assistance is provided to those responsible for the
Andean Condor Conservation Project (Vultur gryphus) to submit a
plan for collaborative management ALPZA species. So adding this
plan to those already established in the region. We are currently
evaluating the plan submitted by the coordinators of the species.
Although delayed, it is planned to continue with the establishment
of Cooperative Management Plan for collared lemur (Varecia
variegata and Varecia rubra), under the MoU with EAZA. We have
an institution and regional coordinator to carry forward the above
mentioned plan. But there are 2 different populations in 4
institutions.
 Restructuring CMCE looking for optimal operation ALPZA
After an evaluation of the past years of development, considering
that to date have submitted 7 SB of the 21 regional posed, that the
participation of institutions is low and is still far from carrying out a
real cooperative management of Latin American collections, seeks,
through restructuring, attract a more direct and active participation
of member institutions, tending to an TAG's similar structure.
Clase
representada
TAG Propuesto
Reptiles
Reptiles
Aves
STB
Pingüinos
Orden
Testudines
Sphenisciformes
Nombre
científico
Familia
Testudinae
Spheniscidae
STB
Nombre científico
Tortuga de Galápagos
Geochelone sp
Pingüino de Humboldt
Responsable
Spheniscus humboldtiPaís
Institución
Tortuga de Galápagos
Aves
Rapaces
Pingüino de Humboldt
Geochelone sp
Ernesto Arbeláez
Amaru
Accipitriformes
Accipitridae
Águila Harpía
Harpia harpyja
Spheniscus humboldti
Guillermo Cubillos
Zoológico Nacional de Chile
Ecuador
Aves
Águila Harpía
Falconiformes
Harpia
harpyja
Cathartidae
VACANTE
Panamá
Cóndor Andino
Aves
Crácidos
Vultur gryphus
Galliformes
Cracidae
Pavón
Cacho
Aves o Pavo dePsitásidos
Guacamayo Militar
Aves
Psitásidos
Oreophasis
derbianus Psittasidae
Juan Cornejo
Psittasiformes
Ara militaris
Frank Camacho
Psittasiformes
Psittasidae
Cotorra Serrana Oriental
Mamíferos
Primates
Mono Araña
Rhynchopsitta terrisi
Primates
Ateles sp
Atelidae
Cotton top tamarín
Mamíferos
Primates
Saguinus
oedipus
Primates
Andrea Echeverry Cotton top tamarín
Zoológico de Barranquilla
Callitrichidae
Saguinus oedipusColombia
Aguará Guazú
Mamíferos
Carnívoros
Nutria Gigante
Argentina
Chrysocyon brachyurus
Stella Maris Velazquez
Zoológico de Buenos Aires
Carnivora
Canidae
Aguará Guazú
Chrysocyon brachyurus
Colombia
Pteronura brasiliensis
Carlos Andrés Galvis
Fundación Zoológica de Cali
Jaguar
Mamíferos
Panthera
onca
Carnivora
Rapaces
Carnívoros
Vanesa Astore
Cóndor Andino
Summit
Argentina
Zoológico de Buenos Aires
Pavón o Pavo de Cacho
Oreophasis derbianus
GuacamayoAfricam
Militar Safari
Africam Safari
Cotorra Serrana Oriental
Carolina Hartmann
Africam Safari
Mono Araña
DEFINIR
Adrián Sestelo
Mustelidae
Carnivora
Felidae Adrián Sestelo
Puma
sp y Leopardus sp
Jaguar
Oso Andino
Mamíferos
Tremarctos ornatus
Carnivora
Felinos Sudamericanos
Felidae
VACANTE
Oso Hormiguero Carnívoros
Mamíferos
Oso Melero
Mamíferos
Edentados y Tapir
Tapir terrestre
Myrmecophaga
tridactyla
Carnivora
Ursidae Flavia Miranda
Tamandua sp.
VACANTE
Pilosa
Myrmecophagidae
Tapirus terrestris
Paula González
Manatí
Mamíferos
Edentados y Tapir
Trichechus
Pilosa manatus
Mamíferos
Edentados y Tapir
Mamíferos
Mamíferos marinos
Ara militaris
Mexico
Mexico
Rhynchopsitta terrisi
Ateles sp
Mexico
Colombia
Argentina
Zoológico de BuenosPteronura
Aires
Nutria Gigante
brasiliensis
Mamíferos
Carnívoros
Felinos Sudamericanos
Carnívoros
Vultur gryphus
Chile
onca
Zoológico de BuenosPanthera
Aires
Argentina
Puma sp y Leopardus sp
Brasil
Oso AndinoMiembro profesional/IBAMA/WCS
Tremarctos ornatus
Argentina
Oso Hormiguero
Myrmecophaga tridactyla
Argentina
Fundación Temaikèn
Venezuela
Carlos Javier SilvaOso MeleroAVZA
Myrmecophagidae
Tamandua sp.
Perissodactyla
Tapiridae
Tapir terrestre
Tapirus terrestris
Sirenia
Trichechidae
Manatí
Trichechus manatus
Genetic Resource Bank in Argentina
Global crisis of biodiversity with species loss due to large-scale human population
growth and exploitation of natural resources.
Reduction in population size and isolation, creating new threats:
- Lack of gene flow
- Loss of genetic variability
- Increase in inbreeding.
Limitations of conventional
breeding programs in
conservation efforts
Physical space
Behavior
Nutrition
Genetic management
Reproductive failure
Health and wellness
It calls us to maximize the utility of
reproductive biotechnologies.
Keeping variability indefinitely for future
generations and exchanging genetic material
between captive and wild populations.
Semen, testicular tissue, ovarian tissue, oocytes, somatic tissue, cells,
blood, serum, hair, leather, urine
Advantage
Maximize space, large number of species and individuals ...
Each individual "survives" in time indefinitely ...
Transport reproductive material and population management ...
Reproductive Assistance
Laparoscopy
ET
ICSI
This makes it possible to overcome physical barriers, behavioral, geographic
isolation, etc..
Genetic rescue
GRB
Regional Referring
First births by Artificial Insemination in an Argentinean ZOO using frozen semen stored
for more than two and a half years in the GRB.
Muflón (Ovis ammon musimon)
Sudamerican Felids Project
Proyecto FELINOS
SUDAMERICANOS
DEVELOPMENT AND TRANSFER OF BIOTECHNOLOGY APPLIED TO
CONSERVATION NATIVE WILDLIFE THREATENED
This allowed us to install, at the zoo, a complete reproductive physiology laboratory.
Reproductive Biotechnology Laboratory
for Wildlife Conservation
Agreement UBA – College of agriculture
- ICSI in Cheeta and Tiger as model.
- Cloning in Chita and Tiger as model.
Development of the technique in not South American species but related of the same lineage.
IVF in domestic cat
ICSI Pantera pardus
ICSI Cheeta - Cat
GENERATION OF INTERSPECIFIC CLONED BLASTOCYSTS BY ZP-FREE NUCLEAR TRANSFER IN WILD FELIDS
L. N. Moro, J. Jarazo, A. Sestelo, D. Salamone
IETS (International Embryo Transfer Society) 2013
Agreement MACN- CONICET
- BRG input samples for population analysis Maned wolf and Pampas deer.
Maned wolf
(Chrysocyon brachiurus)
blood
DNA extraction
PCR
Alelle analysis
Agreement UBA – Veterinary College
-Development in Fallow Deer - sperm metabolism, apoptosis, DNA status.
- Transfer to the pampas deer.
- Generation of graduate course (FVET-ZOO) and master's (2014 FVET).
Capacitation and acrosome reacton induction on thawed Dama dama deer spermatozoa: glycine
effect as cryopreservation diluent supplement
Fernandez, Silvina; Sestelo, Adrian; Rivolta, Miguel and Cordoba, Mariana
Zoológical Science (2013)
Pampas deer
Pampas deer UdeLaR - Uruguay
- 6 years of work, 2 master (Uy), 1 minor thesis on DNA integrity (Ar).
- Transfer of metabolism jobs from developed with C.Vet.
CRIOPRESERVACIÓN DE SEMEN DE VENADO DE LAS PAMPAS
(Ozotoceros bezoarticus): INTEGRIDAD DEL ADN ESPERMÁTICO
POST - CONGELADO
B. Ceballos, F. Beracochea, R. Sapiro, F. Skowronek, J. Gil, S. X. González-Pensado, A.
Sestelo, R. Ungerfeld
9no Simposio Internacional de Reproducción Animal (2011)
Huemul (Hippocamelus bisulcus)
Pudú (Pudu puda)
Birds:
- Start 2011 with seminal extractions PCRAR group (ZOO).
- Milvago chimango and owl as models.
- Transferred to Crowned Eagle, the first time in the world that is
obtained and frozen semen from the species.
Morphometric and Morphologic Description of Crowned Eagle (Harpyhaliaetus coronatus) Spermatozoa: Development
of a Suitable Freezing Protocol, Work in Progress.
L.L. Goudard, *G. Wiemeyer, U. Balsa, M. Encabo, A. Capdevielle, A. Sestelo
I Worldwide Raptor Conference (2013)
Crowned Eagle (Harpyhaliaetus coronatus), sperm characterization.
Agreement College Natural Sciences-CONICET
-Project for obtaining semen from New World monkeys and spermatic coagulum
disrupted by different techniques.
- Tissue samples from BRG and new studies in aging in telomere and metaphase.
- Used for evolution, evolutionary adaptation and population assignment.
- Creating a service for ALPZA.
Comparative sperm morphometry in Neotropical Primates
Steinberg E.R.; Sestelo, A.J.; Lubary, B.; Lopez, L.; Mudry M.D.
II Congreso Latinoamericano XV Congreso Brasileiro de Primatología (2013)
CEBIDAE
Morphology
ATELIDAE
Cebus
libidinosus (CLI)
Alouatta caraya (ACA)
Ateles belzebuth
(ABE)
Cebus nigritus
(CNI)
Ateles
chamek
(ACH)
Education
The huge scope of action covering the projects is necessary to work in partnership
with various national and international institutions such as:
Nationals
•- IByME, (Instituto de Biología y Medicina Experimental)
•- Hálitus
•- Estación Zoológica Experimental, Granja La Esmeralda, Santa Fe
•- Jardín Zoológico y Botánico de La Plata
•- Jardín Zoológico de la Ciudad de Córdoba
•- Jardín Zoológico de Florencio Varela
•- Jardín Zoológico de Batán
•- Parque Zoológico La Máxima, Olavarría
•- Zoo El Paraíso, Sierra de los Padres
•- Reserva Experimental Horco Molle, Tucumán
•- Reserva Fito – Zoológica Dr. Carlos Pellegrini, Tucumán
•- Fundación de Historia Natural Félix de Azara
•- Criadero de Fauna Silvestre ALGAR S. A.
•- Facultad de Veterinaria, Agronomía, Exactas - UBA.
•- Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”
•- Mink’akuy Tawantinsuyupaq
•- Plan Nacional de Conservación del Venado de las Pampas
•- Plan de Conservación del Venado de las Pampas para la Provincia de
Buenos Aires
•- Plan Nacional de Conservación del Yaguareté
•- Dirección Nacional de Fauna Silvestre
•- Coordinación Nacional de Conservación de la Biodivesidad
Internationals
•- Universidad de Castilla, La Mancha, España
•- Museo Nacional de Ciencias Naturales, España
•- Fundación Aquila, España
•- Zoológico del Parque Metropolitano de Santiago, Chile
•- Buin ZOO, Chile
•- Facultad de Veterinaria, Universidad de la República, Uruguay
•- ECFA (Estación de Cría de Fauna Autóctona), Uruguay
•- ALPZA (Asociación Latinoamericana de Parques Zoológicos y Acuarios)
Currently the GRB stored genetic material belonging 385 specimens
representing 83 species.
Exceeding 6,000 samples, including 3800 seminal doses, in addition to
skin biopsy, fibroblasts, blood, serum, hair, seminal plasma, leather
pieces, urine.
These actions are oriented towards the creation of a Biotechnology
Laboratory for Wildlife Conservation, as a center of scientific research
and training in reproductive physiology of wildlife and National
Reservoir of Wildlife Genetic Resources.
Sustainability ... Where are we going?
commitment,
ethics,
coherency ...
Thanks..!