Instructions - LifeNet Health

AngioGRAFT
®
by
Cryopreserved Bio-Implants for Vascular Procedures
Thaw
& Dilution
Instructions
Vascular Bio-Implants
Thaw & Dilution Instructions
Required Sterile Supplies:
• Two (2) major basins (5 liter capacity or greater)
• 1 liter room temperature D5LR Sterile Solution
(Lactated Ringers w / 5% Dextrose)
• Clamp
• Scissors
• Smooth Forceps
1
Step 1
Non-Sterile Team Member:
Using insulated gloves, retrieve/
open the boxed graft and inspect
the pouch to ensure its integrity.
Do not use the graft if the
packaging is damaged.
• 2 liters warm 0.9% normal saline minimum
(additional warm and room temperature saline
may be required to maintain thawing temperature
of 37°C to 42°C).
• 20 cc syringe
• Thermometer
2
3
4
Step 2
Replace the graft in the box,
leave the lid open. Keep at
room temperature for
7 minutes.
Step 3
Remove graft from box, open
outer peel pack and present to
Sterile Team Member.
Step 4
Sterile Team Member:
Double glove and grasp inner
pouch by sealed edge with a
clamp and remove from outer
peel pack.
5
6
7
Step 5
Lower inner pouch into 5 liter
basin containing minimum of 2
liters warmed (37–42˚C) sterile
saline solution.
Step 6
Gently agitate pouch
periodically 5–7 minutes
until ice is melted.
Step 7
When graft is thawed, dry
outside of pouch and open
with scissors.
9
Step 9
Sterile Team Member:
Remove graft with smooth
forceps and place into basin
of D5LR.
10
Step 10
Discard outer gloves and
cut non-cannulated end of
vessel at suture tie to allow
perfusion. Gently flush the
graft with 20 ml of D5LR.
Allow the graft to remain
fully submerged for 10
minutes.
11
Step 11
Gently flush the graft again
with 20 ml of D5LR. The graft is
ready for implantation.
8
Step 8
Non-Sterile Team Member:
Pour 1 liter room temperature
D5LR into second large
sterile basin.
NOTES:
• Use aseptic technique at
all times; prepare each
allograft separately.
• Use sterile thermometer
or sterile warmer to
maintain a temperature
of 37–42˚C throughout
the thawing process.
• If implantation is
delayed, cover basin
with sterile towel and
place basin in larger
basin of sterile slush.
AngioGRAFT
®
Vascular
Saphenous Veins | AngioGRAFT®
Size
Order Code
L = 21 - 30 cm; OD ≥3 mm
CV21-30
L = 31 - 45 cm; OD ≥3 mm
CV31-45
L = 46 - 60 cm; OD ≥3 mm
CV46-60
L = 61 - 70 cm; OD ≥3 mm
CV61-70
L = 71 - 80 cm; OD ≥3 mm
CV71-80
L = 80 cm; OD ≥3 mm
CV>80
Femoral Vessels | AngioGRAFT®
FEMORAL VEINS
FEMORAL ARTERY
Size
Order Code
L = < 21 cm; OD 5–17 mm
FV<21
L = 21 - 30 cm; OD 5–17 mm
FV21-30
L = > 30 cm; OD 5–17 mm
FV>30
Size
Order Code
L = < 21 cm; OD 4–10 mm
FA<21
L = 21 - 30 cm; OD 4–10 mm
FA21-30
L = > 30 cm; OD 4–10 mm
FA>30
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AngioGraft and the LifeNet Health logo are registered trademarks of LifeNet Health, Inc. Virginia Beach, VA
©2015 LifeNet Health. All rights reserved.
68-40-143-01 Rev. 03
All vessels are measured by outside diameter (OD).