ScienMfic achievements from the InfecMous Disease program

ScienMfic achievements from the InfecMous Disease program

SIDA/SAREC‐NICARAGUA
30
YEARS
Scien&fic
achievements
from
the
Infec&ous
Disease
program
BACKGROUND
Scien&fic
publica&ons
in
The
University
strengthening
program
in
UNAN‐León
has
supported
the
development
of
three
scien&fic
programs.
This
poster
presents
the
scien&fic
results
achieved
by
the
Infec&ous
Disease
program
Structure
of
research
4 main areas:
 Infantile diarrhoea
- Bacteria
- Virus
 Parasitic infections
 Antimicrobial resistance
 Molecular biology
Licen&ates
Masters
Corrales Edelma , 1991
Comparison on the performance of
Leishmania major Like and Leishmania
braziliensis As antigen for new world
Leishmaniasis IgGImmunofluorescence test.
Rivera Teresa, 1995
Seroepidemiological and Clinical study
of Chagas
Chagas´´ disease in Nicaragua.
Mayorga-Perez Orlando
Mayorga-Perez
Orlando,, 1996
Prevalence of antibodies to Hepatitis
A, B, C and E viruses in a healthy
population in León, Nicaragua.
Paniagua Margarita, 1997
Analysis of incidence of infection with
Enterotoxigenic Escherichia coli in a
prospective cohort study of infant
diarrhea in Nicaragua.
Scientific publications
2006-2010
2000-2005
1995-1999
1990-1994
1977-1989
0
20
40
60
80
Number of publications
PhD
disserta&ons
at
Karolinska
Ins&tutet
Cáceres, Mercedes, 1999
Cáceres,
Ecological aspects of antimicrobial susceptibility of anaerobic bacteria in Nicaragua
Espinoza, Felix, 2004
Rotavirus in pediatric gastroenteritis in Nicaraguan children
Téllez Sierra, Aleyda
Aleyda,, 2006
Giardiasis in Leon, Nicaragua : Prevalence and protection
Bucardo-Rivera, Filemón
Bucardo-Rivera,
Filemón,, 2008
Pediatric rotavirus and norovirus diarrhea in Nicaragua
Leiva, Byron, 2009
Leiva,
Amoebiasis : Diagnosis and prevalence in León Nicaragua
Vilchez Rugama
Rugama,, Bayardo Samuel, 2009
Molecular and phenotypic characterization of diarrheagenic Escherichia coli from Nicaraguan children
Amaya-Mayorga, Erick, 2010-09-03
Amaya-Mayorga,
Antibiotic resistance in gram negative bacteria affecting children from León, Nicaragua
Reyes-Navarrete,, Daniel, 2010-09-10
Reyes-Navarrete
Escherichia coli flora and diarrhea in Nicaraguan infants
Salazar-Anton, Fernando. 2011-06-17
Immunological and molecular characterization of onchosphere antigens of Taenia solium in Nicaragua
SUMMARY
During
the
years
of
university
strengthening
the
program
has
involved
at
least
100
Nicaraguan
and
Swedish
scien&sts
and
resulted
in
(soon)
8
master
disserta&ons
9
PhD
disserta&ons
63
scien&fic
interna&onal
publica&ons
The
master
program
in
Microbiology
has
now
started
in
León
reviewed
journals
1. Linder E, Marja Isaguilants
Isaguilants,, Mats olsson
olsson,, Lena Lundin
Lundin,, Shervanzindrou
Shervanzindrou,, Edelma Corrales, Aleyda Téllez
Téllez,, Byron Leiva
Leiva,, William
Morales, Teresa Rivera and Jose M. Cabrera. Amebiasis in León, Nicaragua: Entamoebae in stool examination and identification of
Amebic Liver abscess Cases by Serology and PCR. Archives of Medical Research. Vo. 28, Suppl., pp. S314-S316. (1977)
2. Ralamboson D, Le Bras J, Coulanges P, Deloron P, Dulat C, Ramahatra R, Morales W : Preliminary study of the prevalence of malaria
infestation in the Province of Tamatave
Tamatave.. Arch Inst Pasteur Madagascar. 50(1):9-14.(1982)
3. Celeste BJ, Soares Guimaraes MC S, Ma. Corrales E. Peroxidase antibody test for mucocutaneous Leishmaniasis serology
performance indexes and comparison with a fluorescent antibody test. Rev.Ins
Rev.Ins.. Med. Trop. Sao Paulo 30(6):411-414 novembrodezembro.. (1988)
dezembro
4. Tellez A, L. Cortéz
Cortéz,, A. Aust-Kettis
Aust-Kettis,, G. Huldt
Huldt,, J. Jonson and A. Loftenius
Loftenius.:
.: Antibodies against Entamoeba histolytica antigens in sera
from individuals with amoebiasis of different localization. Archives of Medical Research Vo. 21: ( Suppl 1): 63. (1990)
5. Caceres. M., Palmgren
Palmgren,, A-C and Nord C. E. Antimicrobial resistance in human anaerobic microflora in Nicaragua and Sweden.
Spanish Journal of Chemotherapy 4:151-155. (1991) Cáceres
Cáceres.. M., Hedberg
Hedberg,, M., Lindqvist
Lindqvist,, L., Nord, C.E.:Purification and
Characterization of a beta-lactamase
beta-lactamase from Bacteroides distasonis
distasonis.. Journal of Chemotherapy 3(suppl): 121-124. (1991)
6. Soares Guimaraes MC., Celeste BJ, Ma. Corrales E , Antunes C.Comparison on the performance of Leishmania major Like and
Leishmania braziliensis As antigen for new world Leishmaniasis IgGIgG-Immunofluorescence
Immunofluorescence test. Rev. Inst. Med. Trop. Sao Paulo
33(6):503-508. (1991)
7. Téllez A., Cortez. L., Kettis A and Huldt G.Amebiasis in Nicaragua: Class Specific Serum Antibody.:Archives of Medical Research Vo.
23, No. 2, pp 261-264, (1992)
8. Benedito A. Peres, UE. Confloneie
Confloneie.. Pedro. P. Chieffi
Chieffi.. L.M. Gerkien
Gerkien,, Edelma Corrales, Carmen S. Guerra y Marcelo M. Brandao
Brandao.. Estudo
Da Frecuencia de Anticorpos Anti-Toxocara canis em populacho indígena. Rev. Inst. Med. Trop. Sao Paulo 35(Suppl. 10). (1993)
9. Soares Guimaraes MC , Celeste BJ , Ma. Corrales E : Antigenos de L. major-like e L.b. braziliensis Na Reacao de Imnufluorescencia
(IgG-IF) Na Leismaniose Mucocutanea. Rev. Inst. Med. Trop Sao Paulo 35(Suppl.10) (1993)
10. Medina EC, Widmalm G, Weintraub A, Vial PA, Levine MM, Lindberg A: Structural studies of the O-antigenic polysaccharides of
Escherichia coli 03 and the enteroaggregative Escherichia coli strain 17-2.Eur J Biochem 224:191-196.(1994)
11. Ratnayake S, Weintraub A, Medina EC. Structural studies of the c 090 O-antigen polysaccharide.Carbohydr Res, 263:209-215.(1994)
12. Rivera T. Palma G. R. y Morales W. Seroepidemiological and Clinical study of Chagas
Chagas´´ disease in Nicaragua. Rec. Inst. Med. Trop.
Sao Paulo 37(3): 207-213. (1995)
13. Palma-Guzman Rosário
Rosário,, Teresa Rivera B and William Morales G. Domestic vectors of Chagas
Chagas’’ disease in three rural communities of
Nicaragua. Rev. Inst. Med. Trop. Sao Paulo 38 (2): 133-140,(1996)
14. Mayorga
Mayorga-Perez
-Perez Orlando
Orlando,, William Morales, Margarita Paniagua and Orjan Strannegard
Strannegard.. Prevalence of antibodies to Hepatitis A, B, C
and E viruses in a healthy population in León, Nicaragua. Am. J .Trop. Med. Hyg
Hyg.. 55(1): 17-21, (1996)
15. Herrmann Björn
Björn,, Felix Espinoza, Rosaura Rivera Villegas, George Davey Smith, Angela Ramos, Matthias Egger:
Genital chlamydia infection among women in Nicaragua: validity of direct fluorescent antibody testing, prevalence, risk factors and
clinical manifestations. Genitourinary Med. 72: 20-26,(1996)
16. Kjellberg A, Urbina F, Weintraub A, Widmalm G.: Structural studies of the O-antigenic polysaccharide from the enteropathogenic
Escherichia coli O125.Eur J Biochem 239:532-538 (1996)
17. Staaf M, Urbina F, Widmalm G. Structural studies of of the enteroinvasive Echerichia coli (EIEC ) from the enterotoxigenic
Escherichia coli ( ETEC ) O101. Carbohydr Res, 291/127-139.(1996)
18. Espinoza Felix, Margarita Paniagua
Paniagua,, Hans Hallander
Hallander,, Lennart Svensson
Svensson,, Orjan Strannegard
Strannegard.. Rotavirus Infections in young
Nicaraguan Children. Pediatr Infect Disease Journal 16: 564-71,(1997)
19. Espinoza Felix, Margarita Paniagua
Paniagua,, Hans Hallander
Hallander,, K.O. Hedlund and Lennart Svensson
Svensson.. Prevalence and characteristics of severe
rotavirus infections in Nicaraguan children. Annals of tropical paediatrics 17:25-32, (1997)
20. Paniagua M., F. Espinoza, M. Ringman
Ringman,, E. Reizenstein
Reizenstein,, A. M. Svennerholm
Svennerholm,, and H. Hallander
Hallander.. Analysis of incidence of infection with
Enterotoxigenic Escherichia coli in a prospective cohort study of infant diarrhea in Nicaragua. Journal of Clinical Microbiology
35:1404-1410,(l997)
21. Linder Ewert
Ewert,, Marja Isaguliants
Isaguliants,, Mats Olsson, Lena Lundin
Lundin,, Shervan Zindrou
Zindrou,, Edelma Corrales, Aleida Tellez, Byron leiva
leiva,, William
Morales, Teresa Rivera, and Jose M Cabrera. Amebiasis in León, Nicaragua: Entamoebae in Stool Examination and Identification of
Amoebic Liver Abscess Cases by Serology and PCR.Archives of Medical Research 28, Suppl. pp. S314-316, (1997)
22. Tellez A., W.Morales
W.Morales,, T.Rivera
T.Rivera,, E.Meyer
E.Meyer,, B.Leiva
B.Leiva,, E.Linder
E.Linder:: Prevalence of intestinal parasites in the human population of León,
Nicaragua. Acta Tropica 66: 119-125, (1997)
23. Felix Espinoza, Margarita Paniagua
Paniagua,, Hans Hallander
Hallander,, Lennart Svensson
Svensson,, Orjan Strannegard
Strannegard:: Rotavirus Infections in young
Nicaraguan Children.Pediatr Infect Disease Journal, 16: 564-71. ( 1997)
24. Cáceres
Cáceres.. M., Carera
Carera,, E., Palmgren
Palmgren,, A-C and Nord C. E. Antimicrobial susceptibility of anaerobic bacteria from the intestinal microflora
of healthy children and antimicrobial-treated children in Nicaragua. Spanish Journal of Chemotherapy 3:221-228. (1998)
25. Johansen K, Hinkula J, Espinoza F, Levi M, Zeng C, Runden U, Vesikari T, Estes M and Svensson L. Humoral and cell-mediated
immune responses to the NSP4 enterotoxin of rotavirus. Journal of Medical Virology 59:369-377, (1999.)
26. Cáceres
Cáceres.. M., Carera
Carera,, E., Palma, A., Berrios
Berrios,, G., Wintraub
Wintraub,, A., and Nord C. E. Antimicrobial susceptibility of anaerobic and aerobic
bacteria isolated from mixed infections in Nicaragua. Spanish Journal of Chemotherapy 12:332-339 .(1999)
27. Staaf M, Urbina F, Weintraub A, Widmalm G.: Structural elucidation of the O-antigenic polysaccharide from Escherichia coli O21 and
the enteroaggregative Escherichia coli strain 105. Eur J Biochem 266:241-245 (1999)
28. Staaf M, Urbina F, Weintraub A, Widmalm G. Structure elucidation of the O-antigenic polysaccharide from the enteroaggregative
Escherichia coli strain 62D1. Eur J Biochem , 262:56-62 (1999)
29. S. Zindrau
Zindrau,, P. Hagblom
Hagblom,, B. Leiva
Leiva,, Téllez A, JM Cabrera, A. Matute and E. Linder. Antibodies against Entamoeba histolytica hemolisin
HLY4 in sera from patients with invasive amebiasis
amebiasis.. Archives of Medical Research. (2000)
30. Cáceres
Cáceres.. M., Zhang, G., Weintraub
Weintraub,, A., Nord, C.E.: Prevalence and antimicrobial susceptibility patter of Enterotoxigenic Bacteroides
fragilis in children with diarrhea in Nicaragua. Anaerobe, june .6:122-126. (2000)
31. Zindrou S, Orozco E, Linder E, Téllez A, Björkman A. Specific detection of Entamoeba histolytica DNA by hemolysin gene targeted
PCR. Acta Trop. Feb 23;78(2):117-25.(2001)
32. Hallander HO, Paniagua M, Espinoza F, Askelöf P, Corrales E, Ringman M, Storsaeter J. Calibrated serological techniques
demonstrate significant different serum response rates to an oral killed cholera vaccine between Swedish and Nicaraguan children.
Vaccine 21:138-145,(2002)
33. Téllez A, Winiecka-Krusnell J, Paniagua M , Linder E. Antibodies in mother´s milk protect children against giardiasis
giardiasis..
Scand J Infect Dis 35:322-325,(2003)
34. Malmberg Lovisa
Lovisa,, Caroline Fock
Fock,, Felix Espinoza, Filemon Bucardo
Bucardo,, Ann Christine Syvänen and Kåre Bondeson
Bondeson.. Microarrays of
genotyping of human group A rotavirus by multiplex capture and type specific primer extension. Journal of Clinical Microbiology
41(11): 5153-5158, (2003)
35. Lovmar L, Fock C, Espinoza F, Bucardo F, Syvänen AC, Bondeson K. Microarrays for genotyping human group a rotavirus by
multiplex capture and type-specific primer extension. J Clin Microbiol
Microbiol.. Nov;41(11):5153-8 (2003)
36. Mayorga O, M. Egger, M. Zellmeyer and cols. Efficacy of a virosome hepatitis A vaccine in an endemic region in Nicaragua: A
randomized placebo-controlled trial. Journal Infectious diseases (2003)
37. Larsson EA, Urbina F, Yang Z, Weintraub A, Widmalm G. Structural and immunochemical relationship between the O-antigenic
polysaccharides from the enteroaggregative Escherichia coli strain 396/C1 and Escherichia coli O126. Carbohydrate Research, .Jun
1;339(8):1491-6.(2004)
38. Caceres M. Bacteraemia neonatal por Enterobacter multirresistentes productores de Beta-lactamasa
Beta-lactamasa de expectro extendido
extendido..
Revista Médica Latinoamericana (2004
39. Ali T, Urbina F
F,, Weintraub A
A,, Widmalm G
G.. Structural studies of the O-antigenic polysaccharides from the enteroaggregative E coli
strain 522/C1 and the international type strain from Escherichia coli O 178. Carbohydr Res. 340(12): 2010-4,(2005)
40. Bucardo F
F,, Meza-Lucas A,
A, Espinoza F, GarciaGarcia-Jeronimo
Jeronimo RC
RC,, GarciaGarcia-Rodea
Rodea R
R,, Correa D.
D. The seroprevalence of Taenia solium
cysticercosis among epileptic patients in Leon, Nicaragua, as evaluated by ELISA and western blotting. Ann Trop Med Parasitol
Parasitol..
99(1): 41-5, (2005)
41. Tellez A, Palm D,
D, Weiland M, Aleman J,
J, Winiecka-Krusnell J,
J, Linder E,
E, Svard S.
S. Secretory
Secretory antibodies against Giardia intestinalis in
lactating Nicaraguan women. Parasite Immunol
Immunol.. 27(5): 163-9, (2005)
42. Urbina F
F,, Nordmark EL
EL,, Yang Z,
Z, Weintraub A
A,, Scheutz F
F,, Widmalm G
G.. Structural elucidation of the O-antigenic polysaccharide from
the enteroaggregative E coli strain 180/C3 and its immunochemical relationship with E. coli O5 and O65. Carbohydr Res. 340(4): 64550, (2005)
43. Leiva Byron, Marianne Lebbad
Lebbad,, Jadwiga Winiecka-Krusnell
Winiecka-Krusnell,, Isabel Altamirano
Altamirano.,
., Aleyda Tèllez and Ewert Linder. Overdiagnosis of
Entamoeba histolytica and Entamoeba dispar in Nicaragua: A microscopic, Triage Parasite Panel and PCR study. Arch Med Res.
May;37(4):529-34.(2006)
44. Rodríguez-Díaz J, Banasaz M, Istrate C, Buesa J, Lundgren O, Espinoza F, Sundqvist T, Rottenberg M, Svensson L. Role of nitric
oxide during rotavirus infection. J Med Virol
Virol.. Jul;78(7):979-85.(2006)
45. Espinoza F, Bucardo F, Paniagua M, Svensson L, Hallander HO, Bondeson K. Shifts of rotavirus g and p types in Nicaragua--20012003. Pediatr Infect Dis J. Nov;25(11):1078-80. (2006)
46. Ruiz-Palacios GM, Pérez-Schael I, Velázquez FR, Abate H, Breuer T, Clemens SC, Cheuvart B, Espinoza F, Gillard P, Innis BL,
Cervantes Y, Linhares AC, López P, Macías
Macías-Parra
-Parra M, Ortega-Barría
Ortega-Barría E, Richardson V, Rivera-Medina DM, Rivera L, Salinas B, PavíaRuz N, Salmerón J, Rüttimann R, Tinoco JC, Rubio P, Nuñez E, Guerrero ML, Yarzábal JP, Damaso S, Tornieporth N, Sáez-Llorens X,
Vergara RF, Vesikari T, Bouckenooghe A, Clemens R, De Vos B, O'Ryan M; Human Rotavirus Vaccine Study Group. Safety and
efficacy of an attenuated vaccine against severe rotavirus gastroenteritis. N Engl J Med. Jan 5;354(1):11-22.( 2006)
47. Bucardo F, Karlsson B, Nordgren J, Paniagua M, González A, Amador JJ, Espinoza F, Svensson L. Mutated G4P[8] rotavirus
associated with a nationwide outbreak of gastroenteritis in Nicaragua in 2005. J Clin Microbiol
Microbiol.. Mar;45(3):990-7.(2007)
48. Bucardo F, Karlsson B, Nordgren J, Paniagua M, González A, Amador JJ, Espinoza F, Svensson L. Mutated G4P[8] rotavirus
associated with a nationwide outbreak of gastroenteritis in Nicaragua in 2005. J Clin Microbiol
Microbiol.. Mar;45(3):990-7.(2007)
49. Linhares AC, Velázquez FR, Pérez-Schael I, Sáez-Llorens X, Abate H, Espinoza F, López P, Macías
Macías-Parra
-Parra M, Ortega-Barría
Ortega-Barría E, RiveraMedina DM, Rivera L, Pavía-Ruz N, Nuñez E, Damaso S, Ruiz-Palacios GM, De Vos B, O'Ryan M, Gillard P, Bouckenooghe A; Human
Rotavirus Vaccine Study Group. Efficacy and safety of an oral live attenuated human rotavirus vaccine against rotavirus
gastroenteritis during the first 2 years of life in Latin American infants: a randomised, double-blind, placebo-controlled phase III
study. Lancet. Apr 5;371(9619):1181-9.(2008)
50. Bucardo F, Nordgren J, Carlsson B, Paniagua M, Lindgren PE, Espinoza F, Svensson L. Pediatric norovirus diarrhea in Nicaragua. J
Clin Microbiol
Microbiol.. Aug;46(8):2573-80. (2008)
51. Nordgren J, Bucardo F, Dienus O, Svensson L, Lindgren PE. Novel light-upon-extension real-time PCR assays for detection and
quantification of genogroup I and II noroviruses in clinical specimens. J Clin Microbiol
Microbiol.. Jan;46(1):164-70 (2008)
52. Lebbad M, Ankarklev J, Tellez A, Leiva B, Andersson JO, Svärd S. Dominance of Giardia assemblage B in León, Nicaragua. Acta
Trop.Apr;106(1):44-53.(2008)
53. Leiva B, Clasdotter E, Linder E, Winiecka-Krusnell J. Free-living Acanthamoeba and Naegleria spp. amebae in water sources of León,
Nicaragua. Rev Biol Trop. Jun;56(2):439-46.( 2008)
54. Linder E, Lundin M, Thors C, Lebbad M, Winiecka-Krusnell J, Helin H, Leiva B, Isola J, Lundin J. Web-based virtual microscopy for
parasitology:: a novel tool for education and quality assurance. PLoS Negl Trop Dis. ;2(10):e315.(2008)
parasitology
55. Lebbad M, Ankarklev J, Tellez A, Leiva B, Andersson JO, Svärd S. Dominance of Giardia assemblage B in León, Nicaragua.
Nicaragua.Acta
Acta
Trop. Apr;106(1):44-53. (2008)
56. Amaya E, Caceres M, Fang H, Ramirez AT, Palmgren AC, Nord CE, Weintraub A. Extended-spectrum beta-lactamase
beta-lactamase-producing
-producing
Klebsiella pneumoniae in a neonatal intensive care unit in León, Nicaragua. Int J Antimicrob Agents. Apr;33(4):386-7 (2009)
57. Bucardo F, Kindberg E, Paniagua M, Grahn A, Larson G, Vildevall M, Svensson L. Genetic susceptibility to symptomatic norovirus
infection in Nicaragua. J Med Virol
Virol.. Apr;81(4):728-35. (2009)
58. Vilchez S, Reyes D, Paniagua M, Bucardo F, Möllby R, Weintraub A. Prevalence of diarrhoeagenic Escherichia coli in children from
León, Nicaragua. J Med Microbiol
Microbiol.. May;58(Pt 5):630-7.( 2009)
59. Reyes D, Vilchez S, Paniagua M, Colque P, Weintraub A, Möllby R, Kühn I. Diversity of intestinal Escherichia coli populations in
Nicaraguan children with and without diarrhoea. J Med Microbiol
Microbiol.. Dec;58(Pt 12):1593-600. (2009)
60. Vilchez S, Lundborg M, Urbina F, Weintraub A, Widmalm G. Structural studies of the O-antigenic polysaccharides from the
enteroaggregative Escherichia coli strain 94/D4 and the international type strain Escherichia coli O82. Carbohydr Res. Dec
14;344(18):2528-32. (2009)
61. Amaya E, Caceres M, Fanc H, Torres Ramirez A, Palmgren AC, Nord CE, Weintraub A. Antibiotic resistance patterns in gramnegative and gram-positive bacteria causing septicemia in newborns in León, Nicaragua: correlation with environmental samples J
Chemother.. 2010 Feb;22(1):25-9.(2010)
Chemother
62. Nordgren J, Bucardo F, Svensson L, Lindgren PE. Novel Light-Upon-Extension Real-Time PCR Assay for Simultaneous Detection,
Quantification and Genogrouping of Group A Rotavirus. J Clin Microbiol.Mar 10. (2010)
63. Svensson L, Kindberg E, Nordgren S, Carlsson B, Mollby R, Paniagua M. Asymptomatic Norovirus infections in Nicaraguan children
and its association with viral properties and histo
histo-blood
-blood group antigens. Pediatric Infectious Disease Journal accepted 2010