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OmniPur® Grade Research Essential Reagents
OmniPur Grade Research Essential Reagents ® Don’t just rely on good fortune, use high quality Calbiochem® products that you can trust. Omn iPur ® Reag ents OmniPur® Grade Research Essential Reagents are: 1. A comprehensive portfolio of research essential reagents for a wide range of biological applications and techniques OmniPur® grade reagents consist of products such as antibiotics, buffers, detergents, salts, and molecular markers, to name a few. We have carefully selected all products to create an application-focused offering that meets the range of your research needs, such as: • Sample Preparation • Protein Expression, Isolation, and Analysis • Cell and Tissue Culture • Transfection and Selection • Gene Expression • Western Blotting • Electrophoresis 2 2. DNase, RNase, Protease tested for best results DNA, RNA, and proteins are the starting point for most experiments, so using nuclease- and protease-free OmniPur® reagents ensures the integrity of your starting material, improving downstream results. Many other tests are also performed, and are recorded on the Certificate of Analysis to ensure reproducible results, lot after lot. 3. Suitable for research labs as well as production facilities Whether you are performing a single experiment, doing validation tests, or preparing for large-scale production, OmniPur® grade products are available in a wide range of packaging configurations and sizes to fit your needs and to ensure seamless transition from the lab bench to production. OmniPur® Grade Reagents Description Size Amino Acids OmniPur® Glycine 500 g 5 kg 25 kg 50 kg 5 kg 5 kg 4810-500GM 4840-5KG 4860-25KG 4870-50KG 5450-5KG B37022-44 4 L 20 L 500 g 5 kg 25 kg 1 L 4L 1L 100 ea 200 ea 4L 4 L 20 L 1 pack 1 pack 4L 4 L 20 L 200 L 1 pack 100 mL 100 g 500 g 1 kg 5 kg 25 kg 100 g 1 kg 5 kg 50 g 500 g 100 g 5 kg 4L 100 g 1 kg 25 kg 100 g 1 kg 25 kg 500 mL 500 g 5 kg 25 kg 100 mL 6505-4L 6504-20L 9210-500GM 9230-5KG 9260-25KG 6506-1L 6507-4L 6508-1PACK 6500-100TAB 6501-200TAB 8310-4L 8720-4L 8725-20L 8750-1PC 8770-1PACK* 8800-4L 8820-4L 8830-20L 8840-200L* 8850-1PACK 8890-100ML 5310-100GM 5320-500GM 5330-1KG 5340-5KG 5360-25KG* 5380-100GM 5390-1KG 5400-5KG 5710-50GM 5720-500GM 2650-100GM 2670-5KG 8410-4L 6110-100GM 6120-1KG 6150-25KG* 6310-100GM 6320-1KG 6340-25KG* 6350-500ML 9310-500GM 9350-5KG 9380-25KG* 9285-100ML OmniPur® L-Histidine L-Aspartic Acid Buffers OmniPur® 10X PBS Liquid Concentrate OmniPur® TRIS OmniPur® 10X PBS Liquid Concentrate OmniPur® 10X PBS, Premixed Powder OmniPur® PBS Tablets OmniPur® 20X SSC Buffer OmniPur® 10X TAE Buffer OmniPur® 25X TAE Buffer, Premixed Powder OmniPur® 5X TBE Buffer OmniPur® 10X TBE Buffer OmniPur® 10X TBE Buffer TE Buffer Solution OmniPur® HEPES OmniPur® HEPES, Sodium Salt OmniPur® Imidazole OmniPur® BIS TRIS OmniPur® 20X SSPE Buffer OmniPur® MES OmniPur® MOPS OmniPur® 10X MOPS, Concentrate Solution OmniPur® TRIS Hydrochloride OmniPur® TRIS Solution, 1.0M pH 7.5 Catalogue No. Description Buffers (continued) OmniPur® TRIS Solution, 1.0M pH 8.0 OmniPur® TRIS Solution, 1.0M pH 9.0 OmniPur® TRIS Solution, 1.0M pH 10.0 OmniPur® TCEP-HCl OmniPur® 10X TG Buffer OmniPur® TRICINE OmniPur® 10X TG-SDS Buffer OmniPur® TBS 20X Liquid Concentrate OmniPur® TBS 20X Ready Pack Powder OmniPur® 20X SSC Buffer, Premixed Powder OmniPur® 20X SSPE Buffer, Premixed Powder OmniPur® CHES OmniPur® PIPES, Sodium Salt OmniPur® Sodium Citrate, Dihydrate OmniPur® BIS-TRIS Hydrochloride OmniPur® Sucrose Maltose Monohydrate D(+)-Glucose, Monohydrate OmniPur® Dextran Sulfate Sodium OmniPur® Dextran Sulfate, 50% Solution Sterile OmniPur® Ficoll® 400 Chelating Agents OmniPur® EDTA OmniPur® EDTA, Disodium Salt, Dihydrate OmniPur® EDTA, Disodium Salt, Dihydrate, Solution, 5M OmniPur® EGTA OmniPur® 8-Hydroxyquinoline Catalogue No. 100 mL 100 mL 100 mL 2 g 5 kg 4L 500 g 25 kg 4L 4L 2 pack 1 pack 1 pack 100 g 250 g 1 kg 1 kg 5 kg 5 kg 9290-100ML 9295-100ML 9299-100ML 4.85079.0002 4.85079.5000* 9000-4L 9010-500GM 9050-25KG 9015-4L 8320-4L 8325-2PACK 8340-1PACK 8450-1PC 3100-100GM 6910-250GM 6912-1KG 7810-1KG 7860-5KG 4.85070.5000 1 g 5 g 25 g 100 g 500 g 5 kg 100 g 1 kg 10 kg 500 g 5 kg 50 g 1 kg 100 mL 50 g 500 g 100 g 100 mL 500 g 5800-1GM 5810-5GM 5815-25GM 5820-100GM 8510-500GM 8550-5KG MX0160-1 MX0160-7 MX0160-20 4074-2 4074-4 3710-50GM 3712-1KG* 3730-100ML 4550-50GM 4555-500GM* 7410-100GM 8590-100ML SX0850-3 500 g 5 kg 250 g 1 kg 100 mL 500 mL 50 g 100 g 4005-500GM 4008-5KG 4010-250GM 4050-1KG 4055-100ML 4056-500ML 4100-50GM 4690-100GM Carbohydrates OmniPur® IPTG OmniPur® D-(-)-Ribose OmniPur® Sucrose Solution Sorbitol Size *MTO - Product is manufactured upon customer request only. 3 Product Description Size Denaturants OmniPur® Guanidine Hydrochloride 1 kg 5 kg 10 kg 500 g 5 kg 25 kg 250 g 1 kg 5 kg 1 kg OmniPur® Urea OmniPur® Guanidine Isothiocyanate OmniPur® Glyoxal Solution, 40% Catalogue No. 5010-1KG 5030-5KG 5020-10KG* 9510-500GM 9530-5KG 9550-25KG 5120-250GM 5140-1KG 5150-5KG 4910-1KG Product Description Organics and Inorganics (continued) OmniPur® Acrylamide: Bis-acrylamide, 19:1 Premixed Powder OmniPur® Acrylamide: Bis-acrylamide, 19:1, 40% Solution OmniPur® Acrylamide:Bis-acrylamide, 37.5:1, 40% Solution OmniPur® Acrylamide:Bis-acrylamide, 29:1 Premixed Powder OmniPur® N,N'-Methylene Bis Acrylamide OmniPur® X-Gal Detergents OmniPur® Sodium Dodecyl Sulfate, SDS OmniPur® Sodium Dodecyl Sulfate Solution 20% OmniPur® Tween™ 20 (Polyoxyethylene (20) Monolaurate) OmniPur® Tween™ 80 (Polyoxyethylene (20) Monooleate) OmniPur® CHAPS 500 g 200 mL 1L 1 L 4L 1 L 4L 10 g 100 g 100 mL 1 L 4 L 20 L OmniPur® Triton™ X-100 Surfactant OmniPur® Ethidium Bromide OmniPur® Methylene Blue, Trihydrate OmniPur® Silver Pro Stain Kit OmniPur® Xylene Cyanol FF Organics and Inorganics OmniPur® Agarose OmniPur® Agarose PCR Plus OmniPur® Agarose Super-Fine Resolution OmniPur® Agarose, Low Melting OmniPur® Agarose, High Gel Strength OmniPur® Glycerol OmniPur® Acrylamide:Bis-acrylamide, 29:1, 40% Solution OmniPur® Acrylamide OmniPur® Acrylamide Solution, 40% W/V *MTO - Product is manufactured upon customer request only. OmniPur® DNA Extraction Buffer OmniPur ® ACRIDINE ORANGE OmniPur® ABTS Liquid Substrate OmniPur® N,N'-Methylene-Bis-Acrylamide Solution 2% W/V OmniPur® Bradford Method Protein Assay Kit OmniPur® DNase I 50,000 Units EA OmniPur® Enviro-Coat OmniPur® Neutralization Solution OmniPur® Polyethylene Glycol 8,000 OmniPur® Phenylmethyl Sulfonyl Fluoride Dyes and Stains OmniPur® 6X Loading Buffer OmniPur® Ethidium Bromide 4 7910-500GM 7990-200ML 7991-1L 9480-1L 9485-4L 9490-1L 9495-4L 3050-10GM 3055-100GM 9400-100ML 9410-1L 9440-4L 9460-20L OmniPur® TEMED 5 mL 1 g 5g 10 mL 100 g kit 25 g 2100-5ML 4310-1GM 4340-5GM 4410-10ML 6210-100GM 7490-1KIT 9710-25GM 100 g 500 g 25 g 100 g 500 g 100 g 250 g 100 g 100 g 1 L 4L 100 mL 500 mL 1L 100 g 1 kg 5 kg 500 mL 2120-100GM 2125-500GM 2005-25GM 2010-100GM 2020-500GM 2081-100GM 2082-250GM 2070-100GM 2090-100GM 4750-1L 4760-4L 1690-100ML 1700-500ML 1710-1L 1150-100GM 1160-1KG 1170-5KG 1185-500ML Ninhydrin Size Catalogue No. 40 g 200 g 100 mL 500 mL 1L 100 mL 500 mL 200 g 1190-40GM 1200-200GM 1290-100ML 1300-500ML 1310-1L 1490-100ML 1500-500ML 1600-200GM 25 g 100 g 100 mg 1g 20 L 50 g 100 mL 100 mL 2610-25GM 2620-100GM 9630-100MGM 9660-1GM 3700-20L 1125-50GM* 2006-100ML 2640-100ML 1L 1 pack 100 mL 500 mL 2740-1KIT 3750-1PC 4190-100ML 4200-500ML 1L 1 kg 5 kg 6410-1L 6510-1KG 6520-5KG 5 g 250 g 25 mL 100 mL 100 g 10 g 1 kg 7110-5GM 7130-250GM* 8920-25ML 8930-100ML NX0403-1 NX0403-2 NX0403-6 5g 5g 25 g 1 kg 3810-5GM 3860-5GM 3870-25GM 3890-1KG 500 g 5 kg 25 kg 197 g 500 g 5 kg 100 mL 500 g 5 kg 100 g 1 kg 5 kg 1 kg 5 kg 7710-500GM 7760-5KG 7780-25KG PW0002-30 5980-500GM 5982-5KG 5985-100ML 3000-500GM 3004-5KG 3030-100GM 3040-1KG 3045-5KG* 2145-1KG 2149-5KG Reducing Agents OmniPur® DTE OmniPur® DTT (Dithiotreitol) Salts, Acids and Bases OmniPur® Sodium Chloride Chloride Phosphate OmniPur® Magnesium Chloride, Hexahydrate OmniPur® Magnesium Chloride Solution, 1M OmniPur® Calcium Chloride, Dihydrate OmniPur® Cesium Chloride OmniPur® Ammonium Acetate Product Description Salts, Acids and Bases (continued) OmniPur® Ammonium Sulfate OmniPur® Ammonium Persulfate OmniPur® Ammonium Persulfate, Pouches OmniPur® Boric Acid OmniPur® Citric Acid, Anhydrous OmniPur® Sodium Phosphate, Dibasic, Heptahydrate OmniPur® Sodium Phosphate, Monobasic, Monohydrate OmniPur® Potassium Chloride OmniPur® Sodium Acetate, Anhydrous OmniPur® Sodium Acetate, Trihydrate Maleic Acid OmniPur® Lithium Chloride OmniPur® Casamino Acids OmniPur® Cacodylic Acid Size 1 kg 5 kg 25 kg 25 g 100 g 50 ea 1 kg 5 kg 25 kg 1 kg 5 kg 500 g 5 kg 500 g 5 kg 500 g 5 kg 500 g 5 kg 500 g 5 kg 50 kg 500 g 500 mL 500 g 50 g Catalogue No. 2150-1KG 2160-5KG 2170-25KG 2300-25GM 2310-100GM 2400-1PACK 2710-1KG 2720-5KG 2730-25KG 3200-1KG 3220-5KG 8210-500GM 8240-5KG 8290-500GM 8295-5KG 7300-500GM 7320-5KG 7510-500GM 7560-5KG 7610-500GM 7660-5KG MX0100-20 5910-500GM 5922-500ML 2240-500GM 2990-50GM Product Description Solvents OmniPur® Formamide OmniPur® Phenol:Chloroform: Isoamyl Alcohol, 25:24:1 OmniPur® Phenol:Chloroform:Isoamyl Single Phase. 25:24:1 Water OmniPur® Water, DEPC Treated, Sterile, Nuclease-Free Water, WFI Quality, Sterile Purified Water OmniPur® A.C.E. Sequencing Water OmniPur® Water, Sterile, Nuclease Free OmniPur® Water, Sterile, Nuclease Free, Low Endotoxin Size Catalogue No. 100 mL 4L 100 mL 400 mL 100 mL 4610-100ML 4670-4L 6805-100ML 6810-400ML 6820-100ML 100 mL 500 mL 1 L 5 L 10 L 500 mL 1 L 5 L 10 L 20 L 200 L 100 mL 100 mL 5 L 10 L 100 mL 9601-100ML 9602-500ML 9610-1L 9612-5L 9613-10L 4.86505.0500 4.86505.1000 4.86505.5000 4.86505.9010 4.86505.9020 4.86505.9200 2017-100ML 9801-100ML 9811-5L 9812-10L 9820-100ML *MTO - Product is manufactured upon customer request only. Prepare Western Blotting Buffers Using OmniPur® Products Product Lysis Buffer 0.15 M NaCl 5 mM EDTA, pH 8 1% Triton™ X-100 10 mM Tris HCL, pH 7.4 Just before using add: 1:1000 5 M DTT 1:1000 100 mM PMSF in isopropanol (PX1835) 1:1000 5 M aminocaptoic acid 2x Sample Buffer (2105) 130 mM Tris HCL, pH 8.0 20% (v/v) Glycerol 4.6% (w/v) SDS 0.02% Bromophenol blue 2% DTT 8x Resolving Gel Buffer: 100 mL 0.8 g SDS (add last) 36.3 g Tris base (=3 M) Adjust pH to 8.8 with concentrated HCI Catalogue No. 7710-500GM 4005-500GM 9410-1L 9310-500GM 3860-5GM 7110-5GM 9310-500GM 4750-1L 7910-500GM 2830-25GM 3860-5GM 7910-500GM 9210-500GM Product Catalogue No. 4x Stacking Gel Buffer: 100 mL 0.4 g SDS (add last) 6.05 g Tris base (=0.5 M) Adjust pH to 6.8 7910-500GM 9210-500GM 10x Running Buffer: 1 L 30.3 g Tris base (=0.25 M) 144 g Glycine (=1.92 M) 10 g SDS (=1%, add last) Do not adjust pH! 9210-500GM 4810-500GM 7910-500GM 10x Transfer Buffer: 1 L (Cat. No. 9000, ready-to-use) 30.3 g Tris base (=0.25 M) 144 g Glycine (=1.92 M) pH should be 8.3, do not adjust Blocking Buffer: 0.5 L 3% Bovine serum albumin Make up in PBS and sterile filter Then add 0.05% Tween™ 20 Keep at 4 °C to prevent bacterial growth. 9210-500GM 4810-500GM 2905-5GM 6505-4L 9490-1L Stripping Buffer: 0.5 L (Sterile filter solution and keep at 4 °C) 0.2 M Glycine, pH 2.5 4810-500GM 0.05% Tween™ 20 9490-1L 5 To Place an Order or Receive Technical Assistance In Europe, please call Customer Service: Germany: 0800 6931 000 France: 0800 699 620 Italy: 00800 1166 8811 Spain: 00800 1166 8811 Switzerland: 0800 561 470 United Kingdom: 0800 622 935 For other countries across Europe, please call: +44 (0) 115 943 0840 Or visit: www.merckmillipore.com/offices For Technical Service visit: www.merckmillipore.com/techservice Get Connected! Join Merck Millipore Bioscience on your favorite social media outlet for the latest updates, news, products, innovations, and contests! facebook.com/MerckMilliporeBioscience twitter.com/Merck4Bio www.merckmillipore.com/offices Merck Millipore and the M mark are trademarks, OmniPur and Calbiochem are registered trademarks of Merck KGaA, Darmstadt, Germany. Ficoll is a registered trademark of GE Healthcare Biosciences, AB. Tween is a trademark of ICI Americas Inc., Triton is a trademark of Union Carbide Corp. Lit No. PR2219ENEU LS SBU-12-05787 1/2012 Printed in the USA. © 2012 EMD Millipore Corporation, Billerica, MA, USA. All rights reserved.