Kewpie - in-cosmetics Asia

Kewpie
Corporation
Fine Chemical Division
Kewpie strives to provide attractive products to please our customers.
Contents
Product list
01-04
Sodium Hyaluronate
05-06
Hyalo-Oligo®
07-08
Hyaloveil®-P
09-10
Hyalorepair®
11-12
Natural ingredients
13-14
Nutriceutical
15
Chronology of new product launches
16
Products of Kewpie Fine Chemical Division
Ecocert
Chicken
Corn
Fermentation
Seed
Chicken Comb
Chicken egg
Egg Yolk
Egg White
Eggshell Membrane
Egg Yolk Lecithin
Lysozyme
EM Protein
Egg Yolk Lysolecithin
Egg White Peptides
EM Powder
Egg Yolk Oil
Cosmesome series
Hyaluronic acid (Sodium hyaluronate)
Product name
Phytoglycogen
Albumin
Hyaluronic Acid
(Sodium Hyaluronate)
Strict manufacturing management system
guarantees high quality
ISO 9001: 2008 (JIS Q9001: 2008)
ISO 14001: 2004 (JIS Q14001: 2004)
INCI Name
Hyaluronsan
HA-M5070
Hyaluronsan
HA-Q
*
Hyaluronsan
HA-LQ
*
Hyaluronsan
HA-LQH
*
Molecular weight range
（Million Da）
Dose
(%)
0.5－0.7
0.1－1.0
Expiry(condition)
Packing
36 months
(Ordinary temperature)
100g×1
0.53－1.33
Sodium Hyaluronate
0.85－1.6
0.05－1.0
Hyaluronsan
HA-LQSH
24 months
(Ordinary temperature)
1.2－2.2
12 months
(Ordinary temperature)
1.6－2.9
12 months
（1℃～10℃）
100g×1
1kg×1
We also have 1% solution of above *marked products. Please contact us for availability.
Functional hyaluronic acid
INCI Name
Molecular weight range
（Million Da）
Dose
(%)
Hydrolyzed Hyaluronic Acid
Less than 0.01
0.01－1.0
Hyaloveil®-P *
Hydroxypropyltrimonium
Hyaluronate
0.5-0.8
(Reference)
0.005－1.0
Hyalorepair®
C12-13 Alkyl Glyceryl
Hydrolyzed Hyaluronate
Less than 0.01
0.05－1.0
Product name
Hyalo-Oligo®
Goka Factory, Fine Chemical Division
Expiry(condition)
Packing
Remark
Free acid
36 months
(Ordinary temperature)
100g×1
1kg×1
Cationized
Alkylated
We also have 1% solution of above *marked product. Please contact us for availability.
1
2
Eggshell membrane products
Expiry
(condition)
INCI Name
Composition
（%）
EM Protein-P
Hydrolyzed Egg Shell Membrane
100
EM Protein-L
Hydrolyzed Egg Shell Membrane
Butylene Glycol
Methylparaben
Water
1.0
30.0
0.1
68.9
1% solution of EM Protein-P
1.0－20.0
24 months
(1℃～ 10℃
Freezing is strictly
prohibited.)
1kg×1
Promote production of
Type-III Collagen
Egg Shell Membrane Powder
100
Powder made by drying and
crushing eggshell membrane
0.1－1.0
24 months
(Ordinary
temperature)
5kg×2
Emollient effect
Product name
EM Powder (300)
Characteristics
Water soluble powder
being hydrolyzed
Dose
(%)
0.01－0.5
36 months
(Ordinary
temperature)
Packing
Improve dry and rough skin.
Composition
（%）
Characteristics
Dose
(%)
Expiry
(condition)
Packing
Egg Yolk Lecithin
PL-30S
Egg Oil
100
High viscous oil containing
more than 30% of phospholipid
0.1－3.0
12 months
(Under 10 ℃）
1kg×6
Egg Yolk Lecithin
PL-100P
Hydrogenated Lecithin
100
Purified egg yolk lecithin powder
that is stabilized by hydrogenation.
0.05－2.0
36 months
(Under 10 ℃）
1kg×1
Lysolecithin
100
Powder mainly consisted of
lysophosphatidylcholine
that is stable even in
solbilized transparent phase
0.05－2.0
12 months
(Under 10 ℃）
100g×1
3kg×1
Egg Yolk Lysolecithin
LPC-1
Albumin
Egg White Peptides
EP-1
Lysozyme Hydrochloride
Product name
Function
Cosmesome CM-1
Cosmesome L-10
INCI Name
Water
Glycosphingolipids
Phenoxyethanol
Hydrogenated Lecithin
Lysolecithin
Sodium Hydroxide
Citric Acid
Lysolecithin
Glycerin
Dipropylene Glycol
Phytoglycogen
Product name
Phytoglycogen
Moisturizer,
Cosmesome is the pre-mixed preparation of egg yolk lecithin and various cosmetic raw materials
adopted our original technique and can be used for wide range of cosmetics.
Composition
（%）
Characteristics
97.6
1.0
The preparation in which plant
＊
ceramide is dispersed without
＊
using any of synthetic surfactants
＊
nor organic solvents
＊
＊
20
Excellent lysolecithin preparation
as emulsifier, dispersant and
40
solubilizer
40
＊Less than 1.0％
Dose
(%)
Expiry
(condition)
5.0
6 months
(Ordinary
temperature,
Freezing is strictly
prohibited)
0.1－5.0
Packing
1kg×1
12 months
(Ordinary
temperature,
Flammable)
Function
Moisturizer,
emollient,
improver for rough
and dry skin
Moisturizer,
emulsifier,
dispersant
Plant-derived glycogen extracted and refined from sweet corn seed
This Phytoglycogen has a smooth feeling without stickiness and can be used for wide range of cosmetics.
INCI Name
Composition
（%）
Characteristics
Dose
(%)
Expiry
(condition)
Packing
Function
Glycogen
100
Plant-derived glycogen powder
extracted from sweet corn.
0.01－10.0
24 months
(Ordinary
temperature)
1kg×1
Moisturizer
dispersant,
solubilizer,
emolient
Egg white has water retaining function, gives skin elasticity, gloss and protects hair as well.
Lysozyme Hydrochloride has anti-inflammatory effects (mainly for pharmaceutical API).
It can be used for wide range of cosmetics.
★The following products are derived from egg protein.
Egg white products
Product name
Cosmesome
100g×1
INCI Name
Product name
Function
Functional lipid called "Phospholipid" which contains phosphorus in the structure.
Phospholipid plays an important role in sebum/hair and can be used for wide
range of cosmetics.
Egg yolk products
3
Eggshell membrane is a thin film inside the eggshell
★The following products are derived from egg protein.
INCI Name
Composition
（%）
Albumen
100
Characteristics
Dose
(%)
Spray-dried powder that is
the desaccharized egg white
0.1－50.0
0.1－2.0
Hydrolyzed Albumen
100
Powder enzymatically
hydrolyzed egg white with
high heat stability
Lysozyme
100
Enzyme extracted from
egg white, having decomposing
function of mucopolysaccharides
0.001－0.01
Expiry
(condition)
Packing
Function
10kg×1
24 months
(Ordinary
temperature)
36 months
(Ordinary
temperature)
Moisturizer
5kg×2
100g×10
Anti-inflammatory effects
4
This gives moisture to the skin and hair due to
its excellent water holding / moisture retention properties.
List of the patents of
functional hyaluronic acid
Kewpie is the only manufacturer who produces hyaluronic acid by both extraction from chicken comb
and bio-fermentation in Japan.
We have our own special technique to control the molecular weight of hyaluronic acid suited to the
customers' need.
1. High moisture retention
120
100
80
60
40
20
0
0.05
0.1
0.3
conc. of Hyaluronsan HA-LQH（％）
0.5
The group applied 0.1 % solution of Hyaluronsan HA-LQ
The group applied water
* P ＜0.05 (Compare with the condition before applying sample)
25
＊
20
15
5
0
Before application
μs
5
10
20
Time (Minutes)
Test result
It was confirmed that the solution of Hyaluronsan HA-LQH
made the skin moisture content higher in proportion to its
concentration.
Test result
It was confirmed that the solution of Hyaluronsan HA-LQ
increased the skin moisture content over time significantly
and such high content was kept even 20 minutes after the
application.
To increase
adhesiveness of
hyaluronic acid
NO
＊
10
Test method
Applying 0.1% solution of Hyaluronsan HA-LQ onto the subject's
forearm and measured the skin moisture content over time.
(after 5 minutes, 10 minutes and 20 minutes)
Measuring equipment: SKICON-200
To approach
barrier function of
the skin
Are you satisfied with
the hyaluronic acid
currently using ?
YES
What is the molecular
weight of the hyaluronic
acid currently using?
Sodium Hyaluronate
3. Transparency of “Hyaluronsan HA-LQH solution”
Hyaluronsan HA-LQH is a granulated powder
→Easier to be dissolved even with low power stirrer.
→Easier to handle (Hard to airborne)
（ ％）
Absorbance
When charged
5
Hyaluronsan
HA-M5070
(Chicken comb)
0.53 Million ~
1.33 Million Da
Hyaluronsan
HA-Q
(Chicken comb)
0.85 Million ~
1.6 Million Da
Hyaluronsan
HA-LQ
（Fermentation）
1.2 Million ~
2.2 Million Da
Hyaluronsan
HA-LQH
（Fermentation）
1.6 Million ~
2.9 Million Da
Hyaluronsan
HA-LQSH
（Fermentation）
Variety of hyaluronic acids and their functions
100
95
Hyaluronic acid covering the surface
of the skin "Retain Moisture"
Adsorptive type hyaluronic acid
"Adsorb and last"
90
85
80
400nm
(Yellow)
Sebum
660nm (wavelength)
(Turbidity)
Test method
Absorbance of 1% aqueous solution of Hyaluronsan HA-LQH was
measured at 400nm and 660nm.
5 minutes later
0.5 Million ~
0.7 Million Da
We have confirmed high transparency (absorbance) of cosmetic grade
“Hyaluronsan HA-LQH” as follows:
Absorbance of 1% solution
▶
PAT.　JP4845071 B2
PAT.P.　WO2011/102462 A1
To increase
penetration of
hyaluronic acid
Functional
hyaluronic acid
＊
＊
Test method
Applying 0.05, 0.1, 0.3 and 0.5% of the solutions of Hyaluronsan HA-LQH
onto the subject's forearm and measured the skin moisture content
after 5 minutes.
Measuring equipment: SKICON-200
2. Solubility into water
PAT.　JP5241708 B, CN101715457 B
PAT.P.　WO2008/133267 A1
Kewpie's hyaluronic acids meet various demands and keep progress.
Increase amount of moisture in the skin ( )
（％）
The increasing ratio of skin moisture content
140
PAT.　JP4576583 B2, EP1865002 B1
PAT.P.　WO2006/101030 A1
Test result
In each case absorbance in nearly 100%, which proves very
high transparency of Hyaluronsan HA-LQH solution.
Horny layer
Hyaluronic acid reparing
barrier function
Granular layer
Epidermis
Hyaluronic acid penetrating into the
skin"Keep the Moisture Inside"
（Figure）
6
“PENETRATION” type hyaluronic acid
Grade0
Grade1
Grade2
Grade3
0.04
Wrinkles Decrease
Because Hyalo-Oligo covers the skin surface and also penetrates into horny layer, it keeps the skin
moisture both inner and outer side of the skin.
Hyalo-Oligo which remains in horny layer of the skin is not washed away, thus unlike common
hyaluronic acid , it retains the skin moisture even after washing.
3. Effectiveness on the wrinkles
Decreased volume of wrinkles
Hyaluronic acid with the average molecular weight of lower than 10,000 Da.
The nano-size molecule making itself penetrate into horny layer
PAT.　JP4576583 B2, EP1865002 B1
PAT.P.　WO2006/101030 A1
0.02
0
-0.02
-0.04
Start
4th week
Hyalo-Oligo®1％ solution
1. Transdermal absorption
Grade4
Grade5
Grade6
Grade7
* Journal of Japanese Cosmetic Science Society Vol. 30, No. 4
Test method
Applied 1% solution of
twice a day (morning & night)
for 8 weeks. Visual confirmation by the doctor to see wrinkle degree change
(before/ after).
※Consigned testing laboratory: Inforward Inc. Ebisu skin reasearch center
Horny layer
Epidermis
8th week
Water
Decreased volume of wrinkle =
(The grade on the test date) - (the grade on the start date)
Test result
The test result confirmed that Hyalo-OligoⓇ
on wrinkle appearance.
is effective
Dermis
Hematoxylin/eogin dying of skin
※Consigned testing laboratory: BIO alternatives
The result of the test with human skin confirmed
that fluorescent coloring is seen in the horney layer
of skin where Hyalo-Oligo®
has penetrated in.
Unique fluorescence
4. Deep penetration into hair
Test result
It was confirmed that H yalo-Oligo® has
penetrated into the horney layer of skin.
2. High moisture retentivity
Increase amount of moisture in the skin ( )
μs
12
10
Damaged hair (Control)
8
Damaged hair treated with
The shining degree shows the penetration of
6
4
2
0
Before application
1
2
Days after application
3
Common hyaluronic acid
Test method
Bleached and damaged hair was soaked in 1% solution of
with fluorescence label at 40℃ for 15 minutes,
washed, dried and observe this hair section.
Test result
It is confirmed that Hyalo-Oligo penetrated
into the hair inside.
Test method
The gauze soaked in 1% solution of
was attached to the human forearm for
24 hours. Measuring the skin moisture amount after the gauze was detached.
Measuring equipment: SKICON-200
Test result
It was confirmed that Hyalo-Oligo increased twice as much the skin moisture as
common hyaluronic acid.
(Presented at the Pharmaceutical society of Japan, March 2006)
7
8
“ADHESION” type hyaluronic acid
Not easily washing off from skin and hair
due to partial cationization.
PAT.　JP5241708 B, CN101715457 B
PAT.P.　WO2008/133267 A1
3. Adhesion test of Hyaloveil®-P to the hair
Volume adhered to
1gram of hair
In addition to "retaining moisture" and "visco-elsticity" of conventional hyaluronic acid,
"adhesive" feature of Hyaloveil-P makes it possible to stay longer on the hair and the skin and not
easily washes off. "Moisture Veil" of " H yaloveil-" covers the surface of the skin and the hair to
keep it moist even after cleansing or shampoo.
Common hyaluronic acid
1. Adhesion to the skin
Test method
Soaking the damaged hair (bleached hair) into 0.0005%
solution at 40℃ for 10 min. Rinsing with water and drying.
（Analyzed by HPLC）
Adhesion to the skin
Test result
HyaloveilⓇ-P was not washed off but remained more on
the hair attributed by its ionic bonding, compared with
common hyaluronic acid.
Common hyaluronic acid
4. Repair the lift-ups of cuticles of the hair
Test method
Applying the 1% solution of
to the subject’s forearm.
Leaving it at rest for 3 minutes. Washing the sample off the forearm
by running water.
Sticking and stripping adhesive tape. Measuring the residue of
hyaluronic acid in the adhesive tape by HPLC analysis.
Test result
HyaloveilⓇ-P was not washed off but remained more on
the skin attributed by its ionic bonding, compared with
common Hyaluronic Acid.
Electron Micrograph
2. Skin moisture retention test after washing face with cleanser
Increase amount of moisture in the skin ( )
μs
9
5 minutes later
10 minutes later
15
15
10
10
5
5
0
0
-5
-5
-10
-10
Cleanser without any Hyaluronic Acid (Control)
Cleanser with 0.01%
Cleanser with 0.01% common Hyaluronic Acid
Untreated Hair (Control)
Test method
Washing face with the cleanser. Washing the cleanser off by running
water. Removing water from the surface of the skin.
Measuring moisture contents by time (5 min later・10 min later)
using skin surface stratum corneum moisture meter（SKICON-200）
Test result
It was confirmed that skin moisture after washing was
kept and retained by formulating Hyaloveil-P
into facial cleanser.
Treated Hair with
Test method
Soaking the damaged hair (bleached hair) into
1% solution at 40℃ for 10 min. Rinsing with water and drying.
Observation with Scanning Electron Microscope (SEM).
Test result
The lift-ups of cuticles of the hair was repaired on the treated hair
with
10
Hyaluronic acid featuring
“Barrier Function Recovery”
3. Recovery of the barrier function
Brand new hyaluronic acid featuring
barrier function recovery in the skin
PAT.　JP4845071 B2
PAT.P.　WO2011/102462 A1
TEWL (3 days later)
＊
80
Improvement
1. in vitro test / Confirmation of lamella structure formation
＊P＜0.05
100
（％）
Variation
is chemically modified its hydrophobic group by introduction through glycerin
backbone. In addition to "Moisture retention" function, it also has new "barrier function
recovery" one.
60
40
Measurement of transepidermal water loss(TEWL) (※）
20
With water
With
0
Water
1％solution of
1% solution of
sodium hyaluronate
Test result
Hyalorepair
prevents skin moisture from evaporation
(Presented at the Pharmaceutical society of Japan,
March 2012.)
4. Improvement of damaged skin
Number of Maltese Cross = 1
(Average)
▶
Test result
Lamella structure being confirmed
(Circled in red)
2. Approach to Barrier function recovery mechanism
(including hypothesis)
Healthy skin
Dry skin
Observation by microscope photographing (※）
Improvement
Test method
Replicated intercellular condition of dry skin
Added
Microscope photographing
Number of Maltese Cross = 10
(Average)
After damage processing
Test result
It was confirmed that
improved the moisturization of the skin.
(Presented at the Pharmaceutical society of Japan,
March 2012.)
5 days later (applying everyday)
Repaired skin
(formerly dry skin)
Healthy skin: Lamellar layer acts as barrier
to prevent skin from water evaporation.
Dry skin: Out-balanced lamellar layer
causes disorder of barrier function,
resulting in dry skin by water evaporation.
Horny cell
Test method
Damage the forearm skin with mixed solution of acetone and ether.
Apply the 1% solution of
everyday to the damaged skin before bedtime
(※）Testing method of 3 and 4 are the same.
Lipid layer
Water layer
Lamella layer
Lipid layer
"Wedge action"
Water layer
Lipid layer
to joint water
layer and lipid
Repaired skin: Hyalorepair® repairs
out-balanced lamellar layer. When it’s
repaired, the barrier function gets
recovered, which helps skin to retain
moisture.
layer
Horny cell
11
12
Eggshell membrane
Egg yolk phospholipid
Eggshell membrane is a thin inside skin of eggshell and has been used to cure wound for centuries．
It already appeared in “Honzou-koumoku”，a corpus of traditional Chinese medicine compiled in
16th century．“EM Protein” is a hydrolyzed eggshell membrane, which is made from eggshell
membrane and made as soluble inwater by our original production method.
Patented : 1897851
Dissolving
Smooth
feeling
Emulsifying
1. Promotes production of Type-III Collagen
Biocompatibility
Natural
sourced
The structure of Lysolecithin is not the same as lecithin,
Moisture
retention
The characteristic of emulsification is also different from the lecithin.
Lecithin
Papillary layer
Epidermis
Untreated
Coated with EM Protein (0.1μg/cm2)
<Egg Yolk Lysolecithin LPC-1>
Hydrogenated egg yolk lecithin
Lysophosphatidylcholine
- Emulsifier, Emollient agent
- Solubilizer
Lysolecithin
Type-III Collagen
Dermis
Type-I Collagen
Test method
Incubating human dermal fibroblast in Petri-dish for two days
<Egg Yolk lecithin PL-100P>
Subcutaneous tissue
Fibroblast
（sectional model of skin）
Type-III Collagen exists mainly in papillary layer of
dermis, particularly in large volume in fetus’s and baby’s
skin. It decreases by aging, suggesting that it plays an
important roll to keep baby’s skin plump and supple.
2. Increases skin elasticity
Elasticity (R2) variation : an average of 4 test subjects
Phytoglycogen
Recover fibroblast function
1.16
1.1
1.09
1.08
1.07
1.06
1.04
1.02
1
1.08
1.07
1.03
1
1.00
1.03
1.01
0.98
0.96
Start
7days
14days
21days
Treated
28days
Untreated
Absorbance (545nm)
Elasticity (R2) variation
100μg/cm²
1.14
1.12
10μg/cm²
1
1
0.8
0.8
0.8
0.6
0.6
0.6
0.4
0.4
0.4
0.2
0.2
0.2
0
2
3
4
5
6
7
Phytoglycogen
0
2
3
4
5
Untreated
Test method
On the suspension cultured multiplate, prepare the culture bases
of Phytoglycogen to be 0.1, 10 and 100 μg/cm2 to culture
fibroblast.
0.1μg/cm²
1
6
7
0
〈How to count the number of cells〉
Dying fibroblast with crystal violet method and measure
absorbance at 545nm.
Test result
2
3
4
5
6
7
Storage period (Day)
It was confirmed that Phytoglycogen tended to adhere
to human cells and grew human skin fibroblasts.
In other words, it has high biocompatibility.
Test method
Damage the forearm skin of 2 subjects with 9% SDS.
Apply 1% solution of EM Protein-P everyday for 28 days to these 2 subjects and
other 2 subjects who has dry skin. Test result
It was confirmed that damaged skin and dry skin were both
improved.
13
14
Nutriceutical
The egg, from which a chick is hatched, contains all the components needed to create new life.
Kewpie Corporation uses, handles, and sells approximately 200,000 tons of eggs annually, and for over 20 years,
we have extracted and refined biologically active egg components for use in food, cosmetics, and pharmaceuticals
We have applied special techniques to develop methods for extracting and refining these components from a
As you get aged, hyaluronic acid in your skin decreases.
variety of natural resources.
And our products have been used not only in Japan, but also in countries across the world.
Volume of Hyaluronic Acid in the skin (%)
The Decrease of Hyaluronic Acid by Aging
80%
Lost
Chronology of new product launches
e
a
r
1
9
8
2
Age (year)
＊Maria O. Longas, et al, Carbo hydr Res 1987;159:127-136
The improvement of dryness of the skin
Incresed amount of skin moisture (a.u)
Hyaluronic acid　120mg/day
Placebo
16
14
12.7
12
10
8
6
7.9
8.9
6.7
4.9
4
3.0
2
0
Before
intake
Egg Yolk lecithin
Lysozyme Hydrochloride
As you get aged, hyaluronic acid contained in your skin
decreases.
Then, the skin starts to lose its firmness and velvety touch and
you feel aging of your skin.
<Beauty from In & On>
Moisture
retention by
hyaluronic acid
1
9
8
4
Hyaluronic acid for cosmetics use (Extracted from chicken comb )
1
9
8
5
Egg White peptide (Hydrolyzed Egg White)
1
9
8
8
Egg Yolk Lysolecithin
1
9
9
0
Hydrolyzed eggshell membrane
1
9
9
2
Hyaluronic acid for food use (Extracted from chicken comb )
1
9
9
4
Hyaluronic acid for pharmaceutical use (Extracted from chicken comb )
1
9
9
8
Icosapentosan EQ (EPA Ethyl Ester) for pharmaceutical use
2
0
0
1
Condroitin Sulfate
2
0
0
2
House-brand supplement series
Hyaluronic acid made by fermentation method for cosmetics and food use
3 weeks
6 weeks
2week after
intake ends
Test food: Hyaluronsan HA-LF-P supplement (120mg/day)
Placebo: Crystalline cellulose
Test subjects: Japanese female from 35 age to 60 age, 22 persons/group
Test methods: Double-blind test　(parallel 2groups)
Measured item: Measurements of cheek skin moisture
Measured device: CORNEOMETER CM825
Test result
It was confirmed that oral intake of Hyarulonsan HA-LF-P
improved dryness of the skin.
15
Y
Oral intake
【Nutriceutical】
Improves dryness
of the skin
External
application
2
0
0
6
Hyalo-Oligo®
2
0
0
7
Hyaloveil®-P，Hyabest®(J)
2
0
1
2
Hyalorepair®
【Cosmetics】
Relieves the problem
of skin and hair
16