Department of Infectious Diseases and Microbiology

NEWSLETTER
December 2013
Department of Infectious Diseases and Microbiology
Graduate School of Public Health
University of Pittsburgh
IDM’s New Lab Pavilion Opening
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Cover Story p. 1
Report from the Chairman p. 2
Spotlight News p. 3
Bob Yee Interview p. 4
Mary White Interview p. 10
Alumni News p. 13
HRSA Site Visit p. 14
IDM Scholarship Recipients p. 16
IDM Public Health Forum p. 17
Dean’s Day p. 18
IDM Research Day p. 19
IDM Annual Meeting p. 22
Baby News p. 24
Recent IDM Graduates p. 25
Big News p. 29
National & International Oral & Poster
Presentations p. 30
 Recently Published Articles from IDM p. 32
Dr. Velpandi Ayyavoo, Mary White, Dr. Phalguni Gupta,
Dr. Robert Yee, Dr. Charles Rinaldo and
Becky Bosko Marino, IDM PhD Student &
Emilee Knowlton, 2012 IDM PhD Graduate
A message to IDM alumni and friends from Charles R. Rinaldo, Ph.D., Chairman of the
Department of Infectious Diseases and Microbiology
Greetings to alumni, friends and colleagues,
The great news is that the Department has completed its move into our new laboratory
quarters in the GSPH Laboratory Pavilion. This historic event was long in coming – our
last such move was to Parran Hall in 1957! Here are some eclectic tidbits from that year
showing how different we are now, yet how some things never change (NB: I use the
vernacular of that time) – USSR performs atmospheric nuclear test; Israeli forces withdraw
from Sinai Peninsula; US Congress accepts "Eisenhower-doctrine"; 1st electric portable
typewriter placed on sale by Smith Corona in my hometown - Syracuse NY; Buddy Holly
& Crickets (one of my favorite bands) record "That'll Be the Day", Ghana (formerly Gold
Coast) declares independence from UK; thousands of soccer fans riot in Italy; US army
sells last homing pigeons; Best Picture at Academy Awards is the clunker "Around World in 80 Days;" NYC
ends trolley car service; Due to lack of funds, Saturday mail delivery in US is temporarily halted; All NL
baseball teams finally racially integrate, with John Irwin Kennedy being the 1st Negro on the Philadelphia
Phillies; School desegregation law passed, Brown v Board of education; Baseball Commissioner Ford Frick
rules Bing Crosby can keep token stock in the Detroit Tigers, even though he owns part of Pittsburgh Pirates;
Algerian rebels kill 336 collaborators; Tuskegee boycott begins (Negroes boycotted city stores); 1st President to
fly in helicopter - Dwight Eisenhower; British offensive against imam Galeb Ben Ali of Oman; USSR offers
Syria economic/military aid; Cuban dictator Batista bombs Cienfuegos uprising; Pres Eisenhower signs 1st civil
rights bill since Reconstruction and orders US troops to desegregate Little Rock schools; French author Albert
Camus awarded Nobel Prize in Literature; Jailhouse Rock starring Elvis Presley opens; Hand grenade explodes
in Israel's Knesset; USSR launches Sputnik 2 with a dog (Laika); 1st US attempt to launch a satellite fails Vanguard rocket blows up (I saw this happen live on my DeWald B&W TV); Cleveland Browns' Don Paul sets
club record for longest fumble return with a 89-yard run and TD, beating Pittsburgh Steelers 24-0; Simon &
Garfunkel appear on "American Bandstand" as "Tom & Jerry"; Elvis Presley given draft notice to join US
Army; Ed Gein found not guilty by reason of insanity of gruesome murders and grave robbings (an inspiration
for Hitchcock’s “Psycho”); and I cannot resist this one: Philadelphia Phillies’ Richie Ashburn foul balls hit fan
Alice Roth twice in same at bat - 1st one breaks her nose, 2nd one hits her while she is on the stretcher. I cannot
find out what the rest of her life was like…
In 1957, our predecessors led by Drs. Hammon and Parran moved down the hill from their first GSPH quarters
in the Municipal Hospital to a brand new, gleaming set of labs and offices in Parran Hall. We carry their historic
legacy with us over 50 years later to our new
laboratories (new offices are currently under
construction). I trust that many years from now our
professional descendants will move into their new
quarters, looking back to 2013 with the same
measure of honor and pride, and a bit of chuckle as
to our outdated world.
You can see the details and celebration of the
opening of our new labs in this newsletter. A
formal, Pitt Public Health-wide opening of the
Laboratory Pavilion will be held next year (to be
announced).
With kind regards,
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Spotlight News…
IDM’s New Lab Pavilion Opening
Drs. Robert Yee, Phalguni Gupta, Charles Rinaldo and Ms. Mary White
Diana Campbell (IDM PhD Student) and
Emily Frost (IDM Lab Tech)
Debjani Guha (IDM Postdoc) and
Allison Mancini (undergrad)
Ms. Judy Malenka (IDM Administrative Assistant)
Drs. Todd Reinhart, Robert Yee and Charles Rinaldo
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Interview with Dr. Bob Yee
by Mr. Joseph Pawlak
Earlier this Fall the laboratory research in IDM moved from several floors of Parran Hall to the newly
constructed GSPH Lab Pavilion. This is not the first time that IDM moved, having moved from the Municipal
Hospital to Parran Hall in 1957. You were part of that first move. I’d like to get your insights on that, and the
Department in that era.
Question: What brought you to IDM?
It was 1952 and I had just finished my Master’s
degree in the Department of Biological Sciences at
the University of Pittsburgh. I didn’t want to
remain there as a teaching assistant. I heard there
was a position available at the new Graduate School
of Public Health (GSPH). At that time I knew
nothing about public health, but they wanted a
technician. The person that conducted the interview
was Dr. Horace Gezon. The first thing I told him
was that if he could not pay a salary of $3000
annually, I was not interested in the job. He said
that salary was no problem. In those days, that was
a lot of money, and faculty were free to make decisions on their own. I thought that if I ever made $10,000 a
year I would be very happy.
Dr. Gezon was an associate professor in the Department of Epidemiology and Microbiology, studying basic
bacteriology. This was important as I was trained as a bacteriologist and not a microbiologist. Also on the
faculty were William Hammon who was a virologist, Francis Sargent Cheever who was an infectious disease
clinician and Sidney Cobb, a chronic disease epidemiologist who was a full professor. Gezon was interested in
the effects of antibiotics on Shigella. This is the organism that causes bacillary dysentery. This dovetailed with
my interests in bacterial physiology. Dr. Gezon and a member of his group Dr. Sylvia Pan from Shanghai
China, were the only ones really doing basic bacteriology. At that time they were doing techniques that I was
not familiar with. The rest of the department including Dr. Hammon and Dr. Cheever were interested in
isolating etiological agents. Dr. Hammon was a foremost expert in arboviruses. He was also carrying out
national field trials on the effects of gamma globulin in preventing polio. There was no vaccine at that time so
they were using gamma globulin to create passive immunity. Jonas Salk was also located in the municipal
hospital, having been recruited in 1947. When Hammon came to the new GSPH a year later, he knew that
Jonas Salk had the same interest in finding a cure for polio. Hammon met with Salk to discuss how they might
collaborate, but Salk was not interested. It was his nature to be very secretive. I had a classmate who later
became my brother-in-law and worked in Salk’s research group. He was never allowed to talk about his
research, even to his family.
Question: What are some of your recollections of Municipal Hospital?
Municipal was a hospital for individuals with infectious diseases - sort of like a quarantine. It was run by the
city. On the first floor were the diagnostic laboratories. At that time there was no county health department.
Horace Gezon was director of the laboratory. It was from him that I learned about infectious diseases,
epidemiology, and diagnostic microbiology.
Question: That seems like a big difference in the model where you would have a governmental apparatus, in
this case a local government working directly with a research and academic institution. There isn’t anything
like that now.
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It was unusual even at that time because the faculty was primarily interested in research and teaching students.
They were not interested in applied public health. When the city and county wanted to make Horace Gezon a
lab official, the other faculty members discouraged it. So Gezon eventually dropped out of the job, and they
had to find a new director of the laboratories.
Question: You talked about the potential collaboration between Salk and Hammon. Tell me a little more
about that relationship.
They simply did not talk to each other anymore. Hammon decried this missed opportunity and was saddened
about it. One of the things that was fortunate for me was that Jonas Salk taught an animal virology course
which I was able to take. It was taught by him and Jules Youngner. It was a very good course.
Question: So Hammon was working on a non-vaccine treatment for polio?
It was based on the fact that gamma globulin contained antibodies to polio. This was injected into individuals
when there was an outbreak of polio to provide temporary protection against the organism by giving them
antibodies. It was successful but it was only temporary.
Question: Was it in a forerunner to Salk’s work?
No, these were two completely different paths. Hammon’s work was passive immunity while Salk’s work was
active immunity.
Question: Did you have any professional interactions with Salk?
Not really, just to say “hi”. There was very little contact among GSPH people and Salk’s group, although Salk
had a secondary faculty appointment in our department.
Question: What were your recollections of the perceived impact of that announcement of the Salk vaccine in
1955 on the School and the Department? Did you have any thoughts personally?
There was a great deal of excitement and joy in the School and Department, although some reservations were
quietly expressed. Some still favored the Sabin approach, a live vaccine. Others were worried that, at some
point, the lots of the Salk vaccine would contain some live virulent virus. The “kill curve” approached but
never reached zero. I myself welcomed it because one of my undergraduate classmates was crippled by polio
and Bob Rotuno and Don Wegemer, my graduate school classmates, were part of the Salk team. Also I could
say I had taken Animal Virology taught by Jonas and Julie Younger.
Question: Was Dr. Hammon someone you worked closely with during any point in your career?
Yes because Hammon made the whole place like a family. Everyone was treated the same. Socially everyone
was treated the same. He was interested in everyone. One of the great annual events was when the school year
started the faculty, students, and staff would meet at his house for a dinner in the evening and socialization.
This was a total of 25 people or so. In those days we did not have as many students, and all the students were in
their late 20s and early 30s. Some were in their 40s and 50s. Almost all of them were paid by their
organizations. These were professional people who were working on advanced degrees. There were also a lot
of people from other countries. There were a number of students from Japan, Central America, and particularly
Thailand. The school was training future faculty members for other schools of public health in other countries.
Question: What did you do early-on in your career?
My early work involved research in the metabolism of antibiotics against Shigella. Horace Gezon was a
lieutenant commander in the Navy as an epidemiologist. While he was in the Navy he was trying to determine
the effect of ships expelling their waste into the ocean. He was in charge of studying the impact where a
destroyer discharge their waste on coastal waters in North Carolina. They used a bacillus that formed black
colonies to track it. Horace liked to recall that at that point the captain had to turn the ship over to him.
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Horace was not just interested in conventional research. For example, there was an outbreak streptococcus in
the north side of Pittsburgh. He would say he was going to collect some samples, telling me to prepare agar
plates. Horace was very much hands on. That is how I learned about diagnostic bacteriology. At that time he
was well recognized as an expert in epidemiology of infectious diseases. When there was outbreak of
salmonellosis caused in Lancaster, they called on him to investigate it. They believed it was a milk-borne
epidemic. So he took a team that included me and my associate Barney Ferraro who was the head of the city’s
diagnostic laboratory to set up a lab in Lancaster. He also took three or four epidemiology student to participate
in the study. At that time we had so much influence that we received culture media prepared by the Baltimore
biological company. The Maryland state police drove the media to the Pennsylvania border where it was picked
up by Pennsylvania State police and brought to Lancaster. We discovered that the epidemic was caused by a
human carrier. At that time they were using the so-called slow pasteurization method which required constant
stirring. When one worker lifted up the lid to stir the contents of the vat he put his bacterial laden hands on the
inside top of the lid. Condensation from the heating process then dripped into the milk causing the
contamination.
There was another example of an outbreak of “gonorrhea” in southwestern Pennsylvania among elementary
school girls. A local physician diagnosed the discharge of these girls as being associated with gonorrhea. The
physician insisted he didn’t need help and claimed experience with gonorrhea based on the review of dozens of
slides. Upon further investigation we was discovered that the discharge was not gonorrhea, but just a normal
discharge.
Question: You said that you did not have a lot of students in that era. What other differences were there in
terms of your interaction with and observations of students at that time?
In those early days I learned a lot more from the students than I could teach them. They had much more
experience than I did. I remember a time that we were studying an outbreak of gram negative bacteria at Magee
Hospital. There was a student from the Maryland public health department. We needed some containers to
collect samples, and he suggested Dixie cups. I didn’t think Dixie cups survive autoclaving, but to my
amazement they did. In general all the students had more input into their research. However at some point,
public health organizations stopped sending students, because they were hiring students with advanced degrees.
When the younger students started to come in, they required more help from the faculty.
Question: When did you see that change?
Probably in the late 1960s - early 1970s, because I can recall having a lot of discussions with the students about
the war in Vietnam. A lot of these new students had no idea of what public health was. Once a week there was
a public health seminar that everyone was required to attend. Peer pressure, not the threat of any kind of
punishment, got everyone to attend.
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Question: Having just lived through a move of the department, I can tell you it was a traumatic ordeal for
many people. What was the move like in 1957?
The move was smooth. The reason was that we were allocated the space and designed the laboratories. Having
designed the space, we knew where everything was going once it was moved.
In those days (the 1950s) there was only one kind of laboratory in our perception. A lot of the faculty did not
want the architects involved in the design. Even the kitchen (the autoclave facility) was designed by faculty.
Unfortunately the University decided on the furniture for the labs based on the low bid. Some of the laboratory
cabinets were not the best and bench-tops were Formica. We discovered that laboratory burners were putting
out enough heat to make the Formica bubble. We had to put asbestos pads to put under the burners. Another
problem was that the floors were just thick enough to meet the legal specifications. As a result we had to buy
special tables, with heavy tops, because the floor in the lab vibrated whenever someone walked down the hall.
There was also a big debate about whether the building should have central air conditioning, considered a
luxury at that time. Some spaces had window air conditioners. However, this was not practical in labs where
air blasting could cause contamination. At the last minute, the school found a million dollars to put in a Carrier
air conditioning system. This was later discovered to be a mistake, as oil soaked filters in the air conditioning
units captures a lot of dirt which in time began to blow-out of the vents. Ultimately the vents had to be covered
with cheese cloth.
Question: Prior to Parran Hall what was in the space in currently occupies?
It was a combination of residential structures and Fifth Avenue retail stores.
Question: How did people feel about the encroachment of the school into this space?
There was really no concern, primarily because back in the 50s there was a great deal of residential and
commercial space available.
Question: Let’s talk a little more about the faculty that were here in the 1950s. Tell me about the recruitment
of faculty and their development during that period.
In the beginning it was a classic faculty primarily composed of epidemiologists. They were all MDs. PhD’s
who came on board at that time were given the position of research associate. Technically according to
University policy, research associates were actually faculty members. One of my friends during the 1950s
Ernie Ludwick was a research associate virologist. A faculty position opened up for an associate professor
position at Penn State. Ernie interviewed for the position and they were so impressed by him that they offered
him the position as a full professor. It was only later on as research associates did more teaching that we
became recognized as faculty members.
In addition to being the chairman of the department, Hammon and his wife were also missionaries in Africa. I
recall that what they liked least about Africa was the inability to grow tomatoes.
Sarge Cheever was a perfect gentleman, frequently stepping in to resolve the disagreements. He had a
Bostonian accent and an aristocratic bearing. He owned an old De Soto automobile. Around that time Cheever
was appointed vice chancellor for health services. It was also around that time that they recruited Monto Ho
(1959).
Question: What was your degree level at that time?
I received my PhD in 1957 and served as a research fellow. As I became more active I was recognized as a
faculty member.
Question: What did other people think of the new facility?
Everyone loved it.
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Question: What did you think?
I thought it was pretty nice. We had a lot more room, more than twice the space we had in the hospital. For
the first time we got dedicated office space. Prior to that we had a desk in the lab.
Question: In addition to space one of the other reasons for moving to the graduate school of Public health
was to obtain state-of-the-art equipment for the new facility. What were some of the state-of-the-art pieces of
equipment at that time?
One of the most important changes was the modernization of all sterilization equipment. That was a big deal.
Prior to moving to Parran Hall all the dishwashing for the labs was done by hand. At that time this required six
people. We tried to improve the skills of all the people who worked in the autoclave facility. Many of these
people became laboratory technicians including Mary White and Delores Oliver.
I think that at that time we not only wanted to work together but to be together. Today the focus appears to be
more on the individual.
Question: Looking back at the past is sometimes a good point of departure for looking toward the future.
Where do you see public health and perhaps specifically infectious diseases going in the near future? What
are the new frontiers? What are the challenges?
It is hard to say because when I retired, I dropped-out of public health. When I was active I read one journal
article every night before I went to bed. I think it is hard to predict where public health is going today because
there are so many things you cannot do today that you could do in those days. I cannot agree with what John
Cutler did, but he was a great man. Perhaps the biggest issue in public health today is how to deal with the
politics and what is politically correct. You can no longer say you’re going to do something because it is good
for the public.
One of the things you have to learn, even though we were awash in money at that time, was to economize. I
don’t see that anymore. Instead people say that there is not enough money to conduct research. I don’t think
we can continue to use all these sophisticated techniques at such high costs. In five or 10 years, will we have
enough competent scientists to conduct research? The way the environment is today it is discouraging students
from going into pure or academic science. More and more of these individuals are going into industry. The
impact of online teaching also creates additional uncertainty in the field. Graduate training is really about
interaction between students and faculty members. When I was teaching I never answered the students question
directly. Rather I attempted to tease the answer out of them.
When I was active in the Department, it was really bustling; there was always someone rolling a cart down the
hallway. Today the labs seem empty. I still think it takes a strong work ethic to do the things you want to do.
I thought it was interesting that you had to design your laboratories so that people could communicate with each
other. In my days communication occurred naturally. One thing we had in those days was our own cafeteria.
This gave people an opportunity to talk. The Dean always made it a point to sit with different groups.
Another big social event was a picnic at Horace Gezon’s farm where he raised sheep. The entire department
turned out.
In my day there was no bureaucracy. Consequently I could manage the administration, the recruitment,
interviewing, and admissions of students, the development of the curriculum, and consulting with the faculty.
The rest of the faculty did not have to spend time on these matters, leaving more time for research and teaching.
As the Department grew bigger it became increasingly difficult to manage. We wanted Monto Ho to become
department head but he said he didn’t have the time because he was already chief of infectious diseases at
Presby, chief of infection control at Presby and also head of the diagnostic microbiology laboratory at Presby. I
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told Monto that if he took care of the academics I would take care of the administration. We split the job and
were able to work as a team.
Today, I don’t know how I would do it. I think there still exists a schism between the basic researchers and
public health. We used to say that we needed do something to set-up a facade so that we (basic researchers) can
exist in the school of public health. There was always a movement to combine our department with something
else.
Question: In the schism between the basic researchers and public health, where did you come down?
I was on both sides of the fence. I owe this to Horace Gezon. He was doing basic science, but he also got me
into public health. I was fortunate. I came here as a basic bacteriologist/physiologist. I left as still basic, and
also a public health professional.
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Pittsburgh Municipal Hospital for Contagious Diseases was constructed in 1941 as a Public Works
Administration project on land the University of Pittsburgh had given to the city. The 225-bed hospital was
intended to be used primarily to treat communicable diseases. By 1947, antibiotics had virtually eliminated the
need to quarantine patients, and the building was remodeled to house Jonas Salk’s virus research lab in the
basement (formally the morgue). The fourth and fifth floors were active polio wards with patients in iron lungs.
Salk expanded to newly renovated facilities on the first and second floors in 1951, and moved to Scaife Hall
when it opened in 1956. Importantly, the hospital also housed the newly established Graduate School of Public
Health (GSPH) from 1949 to 1957.
William McDowall Hammon, M.D., was the first Chairman of the forerunner of IDM, i.e., Department of
Epidemiology and Microbiology, and a researcher best known for his work on arboviruses. His important work
on passive immunization against poliomyelitis with gamma globulin has been extensively documented by Dr.
Rinaldo (Am J Public Health. 2005; 95:790-799).
Horace M. Gezon, M.D., was a researcher whose work focused on the metabolism and pathogenesis of
shigella, staphylococcus, streptococcus, and paratuberculosis. After a distinguished career in the US Navy, he
served on the faculty of the Department of Epidemiology and Microbiology, and then as a research scientist at
Atlantic Antibodies in Windham, Maine, in the early 1990s.
Francis Sargent Cheever, M.D., served at the University of Pittsburgh as Vice Chancellor of Schools in the
Health Professions, President of the University Health Center of Pittsburgh, Dean of the Medical School, and
professor in the Department of Epidemiology and Microbiology at GSPH.
Silvia Pan, M.D., was a microbiology researcher whose research interests included shigella in Dr. Gezon’s
laboratory. Dr Pan is still active in research in both Taiwan and mainland China.
Sidney Cobb, Ph.D., was an epidemiologist who conducted research ranging from the impact of stress in the
work place to the study of cancer rates near nuclear reactors. Dr. Cobb served on the faculty of the Department
of Epidemiology and Microbiology in the 1950s and 60s.
Julius Youngner, Ph.D., is a virologist and member of the Salk team that developed the polio vaccine. Dr.
Youngner was responsible for establishing techniques to produce poliovirus on a large scale, and helped
develop the process for inactivating the virus for use as a vaccine.
Jonas Salk, M.D., was a virologist who developed the first successful polio vaccine while a faculty member at
the University of Pittsburgh. He left to establish the Salk Institute for Biological Studies in La Jolla, California
in 1960.
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For a sample of the research of Bob Yee and others mentioned in this interview, see the publications below.
This paper was Dr. Yee's PhD thesis:
Yee, R.B., Pan, S.F., and Gezon, H.M. Studies on the metabolism of Shigella. III. The inhibition of the
oxidation of glutamate by Aureomycin. J Bacteriol. 75:56-62, 1958.
Pan, S.F., Yee, R., and Gezon, H.M. Studies on the metabolism of Shigella.1. The occurrence of a tricarboxylic
acid cycle in Shigella flexerni. J Bacteriol. 73:402-409, 1957.
Gezon, H.M., Thompson, D.J., Rogers, K.D., Hatch, T.F., Rycheck, R.R., and Yee, R.B. Control of
staphylococcal infections and disease in the newborn through the use of hexachlorophene bathing. Pediat
51:331, 1973.
Thanks to the alumni and others who have donated to the Bob Yee Scholarship
Fund (see page 16). The establishment of this Fund is one of the greatest
highlights of my career. As a result, we have been able to provide some financial
support to a few of the masters students, especially ones pursuing a career in
public health. We may some day be able to support all of the masters students.
To reach this goal, I hope all of you will become sustaining donors by contributing
$25 annually to the fund. Once again, thank you. I miss the good old days
working with all of you. I remember each and everyone of you.
- Bob Yee
Interview with Mary White
by Mr. Joseph Pawlak
The IDM move from Parran Hall to the GSPH lab Pavilion closes a major chapter in the history of IDM. In
1957, IDM (then known as Department of Epidemiology and Microbiology) moved from space in the
Pittsburgh Municipal Hospital to their new home in Parran Hall. Only a few people can put IDM’s 56 year
tenure in Parran Hall into perspective from first-hand experiences. One of them is Mary White. Mary White
came the department in 1959 and has spent
the past 54 years here.
Question: What brought you to the
Department of Epidemiology and
Microbiology?
My mother was here first and she
recommended me for a job in the
Department. My mom was working in the
department in the kitchen of the school’s
cafeteria. Drs. Gezon and Yee brought my
mom down to Parran Hall when the
department moved here. My first job in the
Department was in the kitchen autoclave
facility. Work in the kitchen was extremely
important to the work in the Department.
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All of the glassware was washed by hand, and at that time before the advent of plastic there was a lot of glass
ware. There was no glassware washer at that time. About 9 people worked in the kitchen.
Question: What did you do in the kitchen?
I washed glassware and operated the sterilizers. Working in the kitchen taught me how important the work was.
The equipment you prepared went into the labs. If it wasn’t done properly, the lab experiment did not work.
Question: Were there any memorable experience from that time in the kitchen?
What I remember most were the people I worked with. I enjoyed everyone around me. I also learned a lot of
things there that helped me when I later worked in the lab.
Question: Was this your first job or did you do something else before you came to Pitt?
Before I came to Pitt, I worked in a 5 and 10 cent store.
Question: How long did you work in the kitchen with you mom?
About two years. Then Dr. Hammon took me out of the kitchen and began to train me for a job in the lab. It
was important to Dr. Hammon to find opportunities for staff to grow into new jobs. This was a great
opportunity for me.
Question: I understand that besides your mom, you had some other relatives who worked in the Department.
Yes, I did. My sister Dolores and my grandson Laron both worked in the Department and both worked in the
autoclave facility.
Question: Tell me a little bit about your first job in the lab.
They put me in a lab with Captain French. He was a veteran who was working on his PhD. He trained me on
how to do a lot of things in the lab. One of the things he taught me was prepare chicken embryos for his
experiments. I worked with Captain French for about 4 years.
After working with Captain French, I worked with Dr. Hammon who taught me how to work with tissue
cultures. I prepared cultures for a number of different people in the department. I continued to do chick
embryos, but also did rabbit kidneys, mouse embryos, and hamster embryos.
Question: What sort of training did you have prior to coming to Pitt?
I was a high school graduate, but did not have any other training prior to coming here. All of the training
related to the job I learned while I was here.
All of my training and all of my subsequent work was done on the lab bench, as there were no biohazard hoods
at that time. It was extremely important that protocols were followed carefully so as not to contaminate an
experiment. Mouth pipetting was also the standard at that time. Again, you had to be very careful when
performing these kinds of procedures.
Question: What did you think of your training?
I thought I was trained very well, and that I caught-on quickly.
Question: Was there anyone who stuck-out in your mind as an exceptional trainer?
After my initial training with George French, the rest of my training was less formal. Essentially I was just
building on those initial techniques.
Question: From your perspective, what were recollections of Jonas Salk? Did you ever meet him?
I never met him and did not know him. My mother met him when she was at municipal hospital, but not me.
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Question: Because of the significance of his work, were people here in GSPH in awe of Jonas Salk?
They never really talked about him that much. Part of the reason was that he never came down to Parran Hall.
Question: What were your reminiscences of that era as it related to the polio epidemic, and the work on the
polio vaccine?
At that time I had two kids. I remember my husband and me taking the kids to get polio shots on the Hill.
Question: What can you tell me about Dr. Hammon?
I really liked Dr. Hammon. He was concerned about everyone who worked for him. He thought of the
Department as a family. Every day 3 o’clock in the afternoon all the workers got together to talk over coffee
and cookies for 15-20 minutes.
Question: Over the past 56 years were there any jobs that you found particularly rewarding, or that
otherwise stuck-out in your mind?
What stands out in my mind was helping the students prepare the tissue cultures that may in some way helped
them to get their degree.
Question: Now you work for Dr. Gupta. How do you like that?
I love it. They saved the best boss for last.
Question: After 56 years on the job what are you looking forward to?
Enjoying my kids and grandkids. As for work, I plan to stay until I feel that it is enough. I love to meet and
work with people from all over the world.
World AIDS Day Proclamation
.
Dr. Linda Frank (pictured center) with Chief County Executive,
Rich Fitzgerald and the Pittsburgh AIDS Commission
on December 2, 2013 in Downtown Pittsburgh.
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Anupama Lochab, 2008 IDM MPH graduate (she is now in internal medicine
residency)
Her husband, 2008 BCHS MPH Graduate (he completed psychiatry residency
and is now practicing in Surrey)
Mark your calendars ……….
for Pitt Public Health Reunion Weekend, May 16-18, 2014. The schedule will include opportunities
for networking, continued education sessions, family activities, and tours of the new lab pavilion.
We are recruiting alumni spanning all departments and years to serve on a planning committee for
the weekend. The committee members will give insights into how we can enhance the experience
for all alumni of Pitt Public Health and assist with outreach to colleagues and classmates. If you’d
like to get involved, please contact Sarah McMullen, Director, Alumni Relations Health Sciences:
[email protected] or 412-647-5307.
13
HRSA, HIV/AIDS Bureau Site Visit
The Pennsylvania/MidAtlantic AIDS Education and Training Center (PA/MA AETC)
The Pennsylvania/MidAtlantic AIDS Education and Training Center (PA/MA AETC) in IDM under the
leadership of Dr. Linda Rose Frank, Associate Professor and Director of the MPH-MIC program completed a
HRSA, HIV/AIDS Bureau Site Visit on June 3-6, 2013. Dr. Frank has been directing the PA/MA AETC since
its initial funding in 1988. The funding for the AETC over the past 10 years has brought more than $38M in
education and training funding for health professionals to improve HIV prevention, treatment, and care the
midatlantic 6 state region of the US. Dr. Frank has led this consortium of 11 Universities to form one of the
most successful AETC programs in the US. The other “performances sites” within the consortium include:
Johns Hopkins University, University of Maryland at Baltimore, Christiana Care in Delaware, Howard
University, Virginia Commonwealth University, INOVA HealthCare Inc., West Virginia University, Drexel
University, University of Cincinnati, and Ohio State University.
The Site Visit team along with Dr. Frank, site directors from participating consortium institutions and
central office staff met for 3 days at the Cathedral of Learning Babcock Room which provided a superb venue
overlooking the beautiful University of Pittsburgh campus. Dr. Donald Burke, Dean of GSPH participated
during the first day offering welcoming and opening remarks to the Site Visit Team and the PA/MA AETC staff
and faculty.
Clinical faculty from the University of Pittsburgh also participated including Dr. Deborah McMahon
from the Pittsburgh AIDS Center for Treatment and Dr. Antoine Douiahy from the Department of Psychiatric
who serve as Co-Investigator on the PA/MA AETC. Clinical faculty from the other universities in the
consortium participated in feedback sessions with the Site Visit Team via Webex.
The Site Visit team rendered a full report to HRSA that includes the following summary and notes many areas
of outstanding achievement.
“The PA/MA AETC Program is outstanding and exemplary in many important regards. The LPSs act as a
Consortium under the leadership of an effective and responsive Central Office. They have established extensive
relationships within their region that allow them to effectively assess needs and develop effective training
strategies. They are viewed as HIV/AIDS policy leaders and agents of change. They have an exceptionally
strong clinical faculty, many of whom participate actively as Co-PIs. PA/MA AETC Central Office has
implemented efficient and effective procedures for collecting program data, for analyzing those data and for
using outcomes measures to guide Program strategy. They have developed innovative web-based program
management and telehealth resources. Through their longitudinal clinical training programs, the PA/MA
AETC has nurtured the development of new HIV clinicians and clinic sites throughout the region. Their success
as an AETC Program is related to their ability to retain and promote high quality individuals, and their ability
to retain high quality staff is related to their cohesiveness as a team. Dr. Frank and her colleagues show a true
passion for their work.” Many of the Program Staff and LPS Site Directors have been with the Program since
its inception, which has promoted a strong sense of teamwork and collegiality.
 The PI (Dr. Frank) actively engages the LPS Site Directors and Co-PIs in the development of PA/MA
AETC policies, strategies and curricula.
 The LPS Site Directors state that they receive highly competent and timely assistance from the Staff of
the PA/MA AETC Central Office.
 The LPS Site Directors state that the Principal Investigator and the PA/MA AETC Central Office Staff
are very responsive in terms of shifting Program resources in response to changing and unanticipated
needs.
 The PA/MA AETC has an outstanding faculty of clinician educators including many who serve as CoPIs at each of the LPSs.
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PA/MA AETC and its LPSs have successfully adapted the AETC goals to highly varied patient and
provider populations in disparate geographic settings.
PA/MA AETC has successfully targeted its training efforts toward minority providers and providers who
serve minority communities in the region.
PA/MA AETC and its LPSs have developed extensive networks of collaboration with health care
providers, community based organizations, health care planning entities and institutions of higher
education within their service areas.
PA/MA AETC has developed and implemented sophisticated web-based program management and
distance learning resources.
PA/MA AETC monitors and analyzes Program outcomes on a quarterly basis, provides timely feedback
to the LPSs, performs thorough analyses of outcomes data and shares these data broadly through
posters, presentations and journal articles.
PA/MA AETC participated actively in region-wide efforts that successfully decreased rates of perinatal
HIV transmission by changing HIV testing laws, implementing routine HIV testing and promoting
linkages to effective HIV medical care.
PA/MA AETC has effectively implemented a “cluster training” strategy to provide educational outreach
and resources to providers in rural areas.
PA/MA AETC is developing strategies and training resources to promote routine HIV testing in dental
care settings.
PA/MA AETC conducts extensive training and clinical support programs in regional correctional
facilities.
Through their Level III and longitudinal training and support strategies, PA/MA AETC has successfully
nurtured the development of many new HIV providers and Ryan White- funded clinics.
PA/MA AETC has worked extensively with regional HIV medical care providers and the National
Quality Center in developing and sustaining effective clinical quality management programs.
Front Row (L-R): Dr. Steve Jenison, Site Reviewer, Andrea Knox, Project Officer, Dr. Linda Frank, PI, Manuel Calas,
Site Reviewer
Second Row: Patricia Lincoln, Bernadine Toye, Jane Russell, Johanna McKee, Leigh Guarinello, Yolanda Wess, Lissa
Foster, Shirley Murphy
Back Row: Dr. Deborah McMahon, Susan Winters, Jeannette Southerly, David Korman, Dorcas Baker, Matt Garofalo
15
IDM Announces 2013 Public Health Scholarship Recipients
Congratulations to Julia Fitzpatrick, Hannah Polglase and Sara Sandrian
Instituted in 2004 to recognize academic excellence among incoming Master of Public Health
and Master of Science students, the IDM Public Health Scholarship has again been awarded
to three new master’s level students. The scholarship is based on academic merit which
includes undergraduate grades and Graduate Record Examination scores. The student’s letters
of recommendation are also reviewed during the selection process. This year’s recipients are:
Julia Fitzpatick – MS
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Undergrad: University of Pittsburgh
Degree: Rehabilitation Science
Hometown: Pittsburgh, PA
Interests: Running & Working Out
Likes about Pittsburgh: Attending sporting events
Hannah Polglase – MPH-PEL
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Undergrad: University of Maryland – College Park
Degree: BA - Anthropology
Hometown: Frederick, MD
Interests: Horseback Riding, Music, Movies
Likes about Pittsburgh: Being in a walkable/
bikeable city
Sarah Sandrian – MPH-MIC
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Undergrad: University of Pittsburgh
Degree: Neuroscience
Hometown: Lebanon, NJ
Interests: Peace Corps, Reading, Yoga
Likes about Pittsburgh: Mt. Washington to see the
city view at night
Support for this scholarship is provided by the Bob Yee Fund in the department. Donations
to this fund can be made by personal check to the University of Pittsburgh, subscript “The
Bob Yee Fund”, and sent to: University of Pittsburgh, Graduate School of Public Health,
Attn: Ms. Robin Tierno, A419B Crabtree Hall, 130 DeSoto Street, Pittsburgh, PA 15261.
Contact Ms. Tierno with questions at: 412-624-3105 or email: [email protected]
16
And the Award Goes To …….
University of Pittsburgh, Graduate School of Public Health
Annual IDM Public Health Forum
March 27, 2013
First Year Poster Session Winners
Kristin Slovenkay (MPH-MIC)
“HIV/AIDS Prevention in Teenagers: Girltalk Program”
Advisor: Dr. Larry Kingsley
and
Jordan Zarone (MPH-PEL)
“Incorporation of C. Difficile Specific Recommendations
into Health Care Facility Hand Hygiene Campaigns”
Advisor: Dr. Jeremy Martinson
Second Year Poster Session Winners
Catherine Howe (MPH-MIC)
“A Literature Review of the Effect of Resource Extraction on Sexually Transmitted Infection Rates and the
Implications for Marcellus Shale Gas Extraction in Pennsylvania: A Template for Prevention”
Advisor: Dr. Linda Frank
Olivia Houck (MPH-PEL)
“Modeling Staffing Dynamics for Pod Operations in an
Infectious Disease Emergency”
Advisor: Dr. Velpandi Ayyavoo
Judges:
Charles Christen, DrPH, Med, Executive Director, Pittsburgh AIDS Task Force
Mack Friedman, PhD, MPH, Director, Project Silk
Mark Stauffer, PhD, Associate Professor of Chemistry; Division Chair, University of
Pittsburgh, Greensburg
17
Award Categories and 2013 Winners
IDM Departmental Awards
Master's Category:
Elsmarie Hormechea, MPH Student
“HPV CONNECTION PROJECT: Increasing Awareness of the Links
between Oral Cancer and HPV”
Advisor: Dr. Linda Frank
Emily Webster, MPH Student
“Overuse of Antibiotics for Asymptomatic Bacteriuria at a
University Teaching Hospital”
Advisor: Dr. Linda Frank
Emily is pictured with Don Burke, MD, Dean of GSPH
Doctoral Category:
Yanille Scott, PhD Student
“Evaluation of broadly neutralizing anti-HIV antibodies for
protection of human mucosal tissue ex vivo”
Advisor: Dr. Charlene Dezzutti
School-wide Awards:
The Center for Public Health Practice Award for Translation
and Application of Research to Public Health Policy and
Practice:
Olivia Houck, MPH Student
“Modeling Staffing Dynamics for POD Operations in an Infectious
Disease Emergency”
Advisor: Dr. Velpandi Ayyavoo
Olivia is pictured with Eleanor Feingold, PhD,
Associate Dean for Education
18
IDM Annual Research Day
September 12, 2013
And the Award Goes To …….
Congratulations to this year’s Poster Winners!!!
PhD Presentations
1st Place:
Colleen Zaccard
2nd Place:
Jana Jacobs
“CD40L Induces Networks of Branched Tunneling
Nanotube-Like Structures Exclusively in Type 1
Polarized Dendritic Cells”
Advisor: Charles Rinaldo, PhD
“RNAI Screening Identifies a Novel Regulator of
MAVS that Suppresses RIG-I-Like Receptor
Signaling”
Advisor: Carolyn Coyne, PhD
3rd Place (tie):
Julianne Baron
3rd Place (tie):
Sreya Tarafdar
“Use of Pyrosequencing to Determine the Effects
of Monochloramine Treatment on Legionella and
Associated Bacterial Populations in a Hospital Hot
Water System”
Advisor: Victor Yu, MD
“NEF Connects HIV-1 to TEC/BTK Kinases in an SH3
Domain-Dependent Manner”
Advisor: Thomas Smithgall, PhD
19
And the Award Goes To …...
MS Presentations
1st Place:
Andrea Dobbs
2nd Place (tie):
Mary Hasek
“Towards Understanding the Expansion of
Plasmablasts in Dengue Infections”
Advisor: Simon Barratt-Boyes, BVSc, PhD
“Addition of Cholesterol-Interacting Motifs to De
Novo Designed Antimicrobial Peptide WLBU2
Enhances Antiviral Activity Against Enveloped
Viruses”
Advisor: Ron Montelaro, PhD
2nd Place (tie):
Nicholas Giacobbi
3rd Place:
Suha Abdelbaqi
“Polyomavirus T Antigen Generates an Antiviral
State”
Advisor: James Pipas, PhD
“Novel Engineered Cationic Antimicrobial
Peptides have Broad-Spectrum Activity against
Francisella Tularensis, Burkholderia Pseudomallei
and Yersinia Pestis”
Advisor: Douglas Reed, PhD
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And the Award Goes To …...
MPH Presentations
1st Place:
Helen McGuirk
2nd Place:
Johanna Rodkey
“Direct Use of TB-Beads in Sputum Samples as a Quick
Method for Detection of TB Infection”
Advisor: Phalguni Gupta, PhD
Postdoc Presentations
“Cost Coverage for the Human Papillomavirus Vaccine
and Successful Completion of the 3-Dose Series: MetaAnalysis”
Advisor: Velpandi Ayyavoo, PhD
1st Place:
Eduardo Nascimento
2nd Place:
Debjani Guha
“Association Between Monocyte Activation and
Regulation of Cell-Surface and Soluble Complement
System Proteins”
Mentor: Ernesto Marques, MD, PhD
“HIV-1 Induced Differential Expression of CXCL5
and its Role in Neuronal”
Mentor: Velpandi Ayyavoo, PhD
6 Judges were assigned to review the posters: 2 for MS’s, 2 for PhD’s, 2 for Postdocs. The Judges were:
MS: Amy Hartman, PhD, Dept. of IDM (Pitt) and Ernesto Marques, MD, PhD, Dept. of IDM (Pitt)
PhD: Anil Ojha, PhD, Dept. of IDM (Pitt) and Nic Sluis-Cremer, PhD, Dept. of Medicine (Pitt)
MPH and Postdocs: Martha Terry, PhD, Dept. of BCHS (Pitt) and Jeremy Martinson, PhD, Dept. of IDM (Pitt)
21
IDM Annual Meeting
North Park
September 13, 2013
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IDM Annual Meeting
North Park
September 13, 2013
23
Baby News . . .
Kevin Melody, IDM PhD Student, and his wife
Lannette welcomed a baby girl, Teresa Elizabeth
on August 10, 2013.
Congratulations to the Melody family.
Aki Hoji, PhD, IDM Postdoc, and his wife Stephanie Bissel, PhD,
both 2005 PhD Graduates of IDM, welcomed their second child
Adelaina Jane on Sept 23. Addie’s big brother Theo loves his little
sister, but as expected, has a bit of jealousy as well.
Congratulations to the Hoji & Bissel family.
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Olivia Houck, Awarded MPH, March 25, 2013
Thesis Title: “Modeling Staffing Dynamics for Pod Operations in an
Infectious Disease Emergency”
Advisor: Dr. Velpandi Ayyavoo
Emily Webster, Awarded MPH, March 25, 2013
Thesis Title: “Overuse of Antibiotics for Asymptomatic
Bacteriuria at a University Teaching Hospital”
Advisor: Dr. Linda Frank
Emily Gage, Awarded MS, April 3, 2013
Thesis Title: “Aerosolized Influenza Infection During Late
Gestation Pregnancy in the Ferret Model”
Advisor: Dr. Kelly Stefano-Cole
Dr. Susan Skledar (Pharmacy),
Dr. Carlene Muto (School of Medicine)
Emily Webster and Dr. Linda Frank (IDM)
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Amy Caroline, Awarded MS, April 4, 2013
Thesis Title: “Characterization of the Humoral
Immune Response in Rats and Non-Human
Primates Exposed to Aerosolized Virulent Rift
Valley Fever Virus”
Advisor: Dr. Amy Hartman
Kimberly Griffin, Awarded MS, April 9, 2013
Thesis Title: “SIV Escape from Immune Recognition Due to Selection Pressure of Immunodominant
CD8+ Epitope Gag-CM9 and Trim5 Restriction Sites”
Advisor: Dr. Michael Murphey-Corb
Dr. Velpandi Ayyavoo (IDM),
Dr. Michael Murphey-Corb (MMG),
Kimberly Griffin and Dr. Jeremy Martinson (IDM)
Carissa Lucero, Awarded PhD, April 10, 2013
Dissertation Title: “Effects of SIV Infection
on Lymphoid Chemokine CXCL13, Paneth
Cell Phenotype and Intestinal Antimicrobial
Peptide Expression”
Advisor: Dr. Todd Reinhart
26
Katherine Kercher, Awarded MS, July 8, 2013
Thesis Title: “Characterization of a gp130 Signaling Receptor Polymorphism”
Advisor: Dr. Frank Jenkins
Jessica Sparks, Awarded MS, July 24, 2013
Thesis Title: “Role of HIV-1 Vpr on Host-Cellular
Functions: Cell-Specific Analysis in ProductivelyInfected Macrophages”
Advisor: Dr. Velpandi Ayyavoo
Gregory Joseph, Awarded MS, August 8, 2013
Thesis Title: “Predictive Biomarkers for the Assessment
of Developing HIV-Associated Neurocognitive Decline in
HIV-Positive Men”
Advisor: Dr. Jeremy Martinson
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Jacob Richards, Awarded MS, September 23, 2013
Thesis Title: “Identifying Genetic Factors for
Promoting Fitness and Rifampicin Tolerance in
Mycobacterium Tuberculosis Biofilms”
Advisor: Dr. Anil Ojha
Emily Nelson, Awarded MPH, November 19, 2013
Thesis Title: “Characterization of T Lymphocyte Phenotype and
Function from Pediatric Heart Transplant Patients who Carry High
EBV Loads and are at Risk for Developing PTLD”
Advisor: Dr. Lawrence Kingsley
Kellie Smith, Awarded PhD (Pitt MVM Program), November 21, 2013
Dissertation Title: “Primary and Recall Cytotoxic T Lymphocyte Responses to Autologous Antigen in
HIV-1-Infected Subjects”
Advisor: Dr. Charles Rinaldo
28
Three New Predoctoral Students have been
Awarded a Fellowship from the
Pitt AIDS Research Training (PART) Program
Todd Reinhart, ScD is leading a group of Pitt faculty engaged in AIDS research who received a 1.1 million
dollar T32 training grant from the NIH for five years. This grant is now in its ninth year (the third year of a five
year renewal). This year the grant will train three new predoctoral researchers, and two have been reappointed
for an additional year in the study of HIV/AIDS to begin August 1, 2013. The PART Program is based on
concepts of interdisciplinary courses and collaborative basic research that provide the foundation for
understanding HIV/AIDS and controlling the epidemic.
Congratulations to this year’s trainees:
Appointed Fellowship 2012/2013
Kevin Melody
Mentor: Zandrea Ambrose, PhD
Research: “In Vitro and In Vivo Drug Efficacy and Resistance of Rilpivirine Long-Acting Formulation”
Taylor Poston
Mentor: Charles Rinaldo, hD
Research: “The Role of CD40L in DC-Mediated HIV-1 trans Infection – CD4+ T cell ‘help’ or harm?”
Zachary Swan
Mentor: Simon Barratt-Boyes, BVSc, PhD
Research: “Delineating the Role of Mononuclear Phagocytes in SIV Disease Control and Progression”
Reappointed 2nd yr. Fellowship
Anthony Cillo
Mentor: John Mellors, M.D.
Research: “Inducing Virion Production from the Latent Proviral Reservoir of HIV-1”
Kevin Raehtz
Mentor: Cristian Apetrei, M.D., Ph.D.
Research: “Study of the Early Events after Mucosal Transmission of SIVagm in African Green Monkeys
(AGMs)”
29
National & International
Oral & Poster Presentations
Ambrose Z. Using a nonhuman primate model to study viral reservoirs and persistence.
Oral presentation and session chair at the 31st Annual Symposium on Nonhuman Primate
Models for AIDS, Atlanta, GA, November 3-6, 2013.
Ayyavoo V. Human Immunodeficiency virus –1 (HIV-1) induced host cellular
transcriptional regulation; Lessons learned. Oral Presentation, University of El Paso, TX, Nov. 15, 2013.
Berendam SJ, Ambrose Z, Kline CJ, Shen C, Gupta P, Murphey-Corb MA, Reinhart TA. Functional characterization of primary
rhesus lymphatic endothelial cells (LECs) and its susceptibility to simian immune deficiency virus (SIV) infection. Science 2013—
Convergence, Pittsburgh, PA, Oct. 2-4, 2013.
Berendam SJ, Murphy-Corb MA, Fuller DH, Reinhart TA. Isolation of primary pulmonary rhesus and ferret lymphatic
endothelial cells (LECs) and their functional respond to stimulation with multiple toll-like receptors ligands. Pittsburgh International
Lung Conference, Pittsburgh, PA, Oct. 17-18, 2013.
Berendam SJ, Ambrose Z, Kline CJ, Shen C, Gupta P, Murphey-Corb MA, Reinhart TA. Innate Immune Sensing and SIV
Infection of Primary Rhesus Macaque Lymphatic Endothelial Cells. 31st Annual Symposium on Nonhuman Primate Models for
AIDS, Atlanta, GA, Nov. 3-6, 2013.
Boscia A, Akabori K, Benamram Z, Michel J, Steckbeck J, Jablin M, Montelaro R, Nagle J, Tristram-Nagle. Structural basis for the
dual role of LLP-2 on the cytoplasmic tail (CTT) of HIV-1 gp41. Ann. Mtg. Biophysical Society. Philadelphia, PA, 2013.
Friedman MR, Feliz N, Brookins M, McGeorge T. Project Silk: A demonstration project for young MSM and transgender people of
color. National African American MSM Leadership Conference on HIV/AIDS and other Health Disparities, Los Angeles, CA, 2013.
Friedman MR. Responding to the HIV prevention and care needs of African American YMSM: a community-based participatory
programming approach. Pitt Men’s Study HIV Education Forum, Pittsburgh, PA, 2013.
Friedman MR, Herrick AL, Krier S, Silvestre AJ. Project Silk: A Multilevel HIV Prevention Intervention for African-American
YMSM and YTG. The Future of HIV Prevention and Treatment: Integrating Innovative Methods with Intervention Science Meeting,
Bethesda, 2013.
Friedman MR, Dodge BM, Schick V, Goncalves G, Herbenick D, Hubach RD, Reece M. Not a legitimate sexual orientation:
Attitudes toward bisexual men and women as a risk factor for HIV/AIDS. Oral presentation. American Public Health Association
Conference, Boston, 2013.
Friedman MR, Wei C, Klem ML, Silvestre AJ, Markovic N, Stall RD. HIV infection among men who have sex with men and
women (MSMW): A systematic review andcomprehensive meta-analysis. Oral presentation. American Public Health Association
Conference, Boston, 2013.
Guha, D. HIV-1 induced differential expression of CXCL5 and its role in neuronal survival. 12 th International Society for
NeuroVirology (ISNV), Washington, DC, Oct. 29 – Nov, 2, 2013.
Hartman AL, Powell DS, Bethel LM, Caroline AL, Bales JM, Schmid RJ, Oury TD, Reed DS. Efficient induction of viral
encephalitis after aerosol exposure to Rift Valley Fever Virus. 2013 Negative Strand Virus Meeting. Granada, Spain. June 16-21,
2013.
Kline C, Kearney MF, Ndjomou J, Anderson EM, Kordella D, Shao W, Coomer C, Spindler J, Mellors JW, Coffin JM, Lifson JD,
Ambrose Z. Similar viral populations persist in multiple tissues during suppressive antiretroviral therapy in a macaque model of
HIV/AIDS. Invited speaker at the Annual Case Western Reserve University Center for AIDS Research (CFAR) Conference,
Cleveland, OH, August 12-13, 2013.
30
Kline C, Ambrose Z. Some dysregulated immune responses in lymph nodes and gut during RT-SHIV infection are not restored to
normal after suppressive ART. Poster presentation at 6th International Workshop on HIV Persistence during Therapy, Miami, FL,
December 3-6, 2013.
Knowlton, E. and Rinaldo C. Human herpesvirus 8 Infection induces polyfunctional B lymphocytes producing cytokines and
chemokines that drive Kaposi’s Sarcoma. ICMAOI, Bethesda, MD, Nov. 12-13, 2013.
Kuhlmann A, Steckbeck J, Sturgeon T, Craigo J, Montelaro R. The C-terminal tail of HIV-1 envelope: a unique role for conserved
LLP arginines in Env functional properties. Ann. Retroviruses Mtg, Cold Spring Harbor Lab, NY, 2013.
Lashua L, Deslouches B, Pilewski, J, Montelaro R, Bomberger J. Engineered cationic antimicrobial peptides (eCAP) prevent and
disrupt biotic Psudomonas aeruginosa biofilms on airway epithelial cells. North American Cystic Fibrosis Conference, Salt Lake City,
UT, 2013.
Lepone L, Rappocciolo G, Piazza P, Knowlton E, Jenkins F, Rinaldo C. CD4 regulatory T cells control CD8 T cell responses to
human herpesvirus 8 lytic and latency proteins. AAI Annual Meeting, Honolulu, HI, May 2013.
Liu C, Cook S, Craigo J, Cook R, Issel C, Montelaro R, Horohov D. The kinetics of envelope-specific cellular immune responses in
equine infectious anemia virus-infected ponies. Immunology 2013 Conference, Honolulu, HI, May 2013.
Macatangay BJC, Lawani MB, Wheeler ND, Riddler SA, Whiteside TL, Bedison A, Rinaldo CR, Mellors JW. Dendritic cell-based
HIV Therapeutic Vaccination Increases Residual Viremia in Individuals on Antiretroviral Therapy. Sixth International Workshop on
HIV Persistence During Therapy, Miami, FL, Dec. 3-5, 2013.
Mailliard RB, Smith KN, Fecek RJ, Rappocciolo, G., Watkins SC, Mullins JI, Rinaldo CR. Exploitation of cross-reactive CTL
‘help’ as a potential immune escape strategy for HIV-1 [abstract]. Immunology 2013 Conference, Honolulu, HI. May 2013.
Mailliard RB, Smith KN, Fecek RJ, Rappocciolo, G., Watkins SC, Mullins JI, Zaccard CR, Rinaldo CR. All bark and no bite:
HIV exploitation of CTL ‘help’ in the absence of killing. Sixth International Workshop on HIV Persistence During Therapy, Miami,
FL, Dec. 3-5, 2013.
Piazza P, Campbell DM, Mailliard R, Marques E, Hildebrand WH, Buchli R, Rinaldo CR. Dengue NS3 Protein Elicits
Heterotypic CD8 Memory T Cell Responses To Dominant and Sub-Dominant Epitopes [abstract]. MASIR 2013 Conference.
Dubrovnik, Croatia. May 29-June 1, 2013.
Rappocciolo G, Jais M, Garcia-Exposito L, Piazza P, Gupta P, Rinaldo CR. Profound Alterations in Cholesterol Metabolism
Restrict HIV-1 Trans Infection of CD4 T Cells in Viremic Controllers. Sixth International Workshop on HIV Persistence During
Therapy, Miami, FL, Dec. 3-5, 2013.
Reed DS, Powell DS, Bethel LM, Caroline AL, Trichel AM, Oury TD, Hartman AL. Fatal encephalitis in African green monkeys
and common marmosets after aerosol infection with Rift Valley Fever virus. 2013 Negative Strand Virus Meeting. Granada, Spain.
June 16-21, 2013.
Sankapal S, Gupta P, Ratner D, Cu-Uvin S, Caruso L, Ding M, Coyne C, Chen Y. Unraveling Mechanism of HIV-1 Transmission
across Mucosal Epithelia. American Society for Reproductive Immunology. Boston, MA, May 28 - Jun 2, 2013.
Sankapal S, Gupta P, Ratner D, Caruso L, Ding M, Coyne C, Cu-Uvin S, Ramratnam B, Chen Y. Early events in HIV
transmission through cervical and rectal mucosa. American Society for Reproductive Immunology. Boston, MA, May 28 - Jun 2,
2013.
Congratulations to Soni Sankapal who was awarded a Travel Award from the Conference.
Sanyal A, Ratner D, Gupta P. The role of sexually transmitted infections on the antiviral properties of microbicides. American
Society for Reproductive Immunology. Boston, MA, May 28 - Jun 2, 2013.
Schauer G, Sluis-Cremer N, and Leuba S. Mechanism by which K103N in HIV-1 Reverse Transcriptase Confers Efavirenz
Resistance Revealed by Single Molecule Fluorescence Spectroscopy. 20 th Conference on Retroviruses and Opportunistic Infections
(CROI 2013). Atlanta, GA, USA. March 3-6, 2013.
Sluis-Cremer N, Huber K, Brumme C, Wallis C, Mellors J and Harrigan PR. The E138A mutation in HIv-1 reverse transcriptase is
more common in subtype C than B and decreases susceptibility to NNRTIs. 20th Conference on Retroviruses and Opportunistic
Infections (CROI 2013). Atlanta, GA, USA. March 3-6, 2013.
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Smith KN, Mailliard RB, Jiang W, Ratner D, Gupta P, Mullins JI, Rinaldo CR. The effect of regulatory T cells on antigen
specific responses increases with progression to AIDS. Molecular Virology and Microbiology Research Symposium, Pittsburgh, PA,
April 29, 2013.
Smith KN, Mailliard RB, Jiang W, Ratner D, Gupta P, Mullins JI, Rinaldo CR. T cell responses to autologous antigen in chronic
HIV-1 infection are masked by regulatory T cells and revealed by dendritic cell. Measurement of Antigen Specific Immune
Responses (MASIR), Dubrovnik, Croatia, May 31, 2013.
Smith KN, Mailliard RB, Jiang W, Ratner D, Gupta P, Mullins JI, Rinaldo CR. Teaching new dogs new tricks: an in vitro model
of dendritic cell immunotherapy induces CTL from naïve precursors in subjects on ART. Towards an HIV Cure Symposium, Kuala
Lumpur, Malaysia, June 29, 2013
Steckbeck J, Deslouches B, Montelaro R. Sequence optimized antibiotic peptides-SOAPs. University Research and
Entrepreneurship Symposium 2013. Cambridge, MA, 2013.
Steckbeck J, Deslouches T, Montelaro R. Sequence optimized antibiotic peptides for the treatment of infectious diseases. BIO,
Chicago, IL, 2013.
Xu H, Franks T, Gibson G, Huber K, Aiken C, Watkins S, Sluis-Cremer N, Ambrose Z. Evidence for biphasic uncoating during
HIV-1 infection from a novel imaging assay. Poster presentation at the Annual Structural Biology Related to HIV/AIDS Meeting,
Bethesda, MD, June 20-21, 2013.
Zaccard CR, Mailliard RB, Kalinski P, Ayyavoo V, Salter RD, Watkins SC, Rinaldo CR. CD40L induces networks of branching
tunneling nanotube-like structures exclusively in type 1 polarized dendritic cells [abstract]. Immunology 2013 Conference, Honolulu,
HI. May 2013.
Zerbato J and Sluis-Cremer N. Development of a primary cell model of HIV-1 latency in naïve CD4+ T cells. International
Workshop on HIV & Hepatitis Virus Drug Resistance and Curative Strategies. Toronto, Canada, 4-7 June 2013.
Recently Published Articles
From the Department of IDM
Abbas UL, Glaubius R, Mubayi A, Hood G, Mellors JW. Antiretroviral
therapy and pre-exposure prophylaxis: combined impact on HIV
transmission and drug resistance in South Africa. J Infect Dis. 208:224234, 2013.
Andrade A, Rosenkranz SL, Cillo AR, Lu D, Daar ES, Jacobson JM, Lederman M, Acosta EP, Campbell T, Feinberg J, Flexner C,
Mellors JW, Kuritzkes DR; AIDS Clinical Trials Group A5248 Team. Three distinct phases of HIV-1 RNA decay in treatment-naive
patients receiving raltegravir-based antiretroviral therapy: ACTG A5248. J Infect Dis. 208:884-891, 2013.
Aziz N, Margolick JB, Detels R, Rinaldo CR, Phair J, Jamieson BD, Butch AW. Value of a quality assessment program in optimizing
cryopreservation of peripheral blood mononuclear cells in a multicenter study. Clin Vaccine Immunol. 20:590-595, 2013.
Boscia AL, Akabori K, Benamram Z, Michel JA, Jablin MS, Steckbeck JD, Montelaro RC, Nagle JF, Tristram-Nagle S.
Membrane structure correlates to function of LLP2 on the cytoplasmic tail of HIV-1 gp41 protein. Biophys J. 105:657-666, 2013.
Choi SM, McAleer JP, Zheng M, Pociask DA, Kaplan MH, Qin S, Reinhart TA, Kolls JK. Innate Stat3-mediated induction of the
antimicrobial protein Reg3¿ is required for host defense against MRSA pneumonia. J. Experimtl. Med. 210:551-561, 2013.
32
Choi YK, Fallert Junecko BA, Klamar CR, Reinhart TA. Characterization of cells expressing lymphatic marker LYVE-1 in
macaque large intestine during simian immunodeficiency virus infection identifies a large population of nonvascular LYVE-1(+)/DCSIGN(+) cells. Lymphatic research and biology. 11:26-34, 2013.
Courtney Z, Domling A, Ayyavoo V. Development of a robust cell-based high-throughput screening assay to identify targets of HIV1 Vpr dimerization. Drug Des Devel Ther. 7:403-412, 2013.
Craigo JK, Ezzelarab C, Cook SJ, Chong L, Horohov D, Issel CJ, Montelaro RC. Envelope determinants of equine lentiviral vaccine
protection. PLoS One. 8:e66093, 2013.
Crawford KW, Li X, Xu X, Abraham AG, Dobs AS, Margolick JB, Palella FJ, Kingsley LA, Witt MD, Brown TT.
Lipodystrophy and inflammation predict later grip strength in HIV-infected men: the MACS Body Composition substudy.
AIDS Res Hum Retroviruses. 29:1138-1145, 2013.
de Melo AB, Nascimento EJ, Braga-Neto U, Dhalia R, Silva AM, Oelke M, Schneck JP, Sidney J, Sette A, Montenegro SM,
Marques ET. T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple
HLA-I and -II binding motifs. PLoS Negl Trop Dis. 7:e1938, 2013.
Deslouches B, Steckbeck JD, Craigo JK, Doi Y, Mietzner TA, Montelaro RC. Rational design of engineered cationic antimicrobial
peptides consisting exclusively of arginine and tryptophan, and their activity against multidrug-resistant pathogens. Antimicrob
Agents Chemother. 57:2511-2521, 2013.
D’Souza G, Rajan S, Bhatia R, Cranston RD, Plankey MW, Silvestre AJ, Ostrow DG, Wiley, D, Shah N, Brewer NT. Uptake and
Predictors of Anal Cancer Screening in Men Who Have Sex with Men. American Journal of Public Health. 103:e88-e95, 2013.
Duskova K, Nagilla P, Le HS, Iyer P, Thalamuthu A, Martinson J, Bar-Joseph Z, Buchanan W, Rinaldo C, Ayyavoo V. MicroRNA
regulation and its effects on cellular transcriptome in Human Immunodeficiency Virus-1 (HIV-1) infected individuals with distinct
viral load and CD4 cell counts. BMC Infectious Diseases. 13:250, 2013.
Founds SA, Fallert-Junecko B, Reinhart TA, Parks WT. LAIR2-expressing extravillous trophoblasts associate with maternal spiral
arterioles undergoing physiologic conversion. Placenta. 34:248-255, 2013.
Friedman MR, Stall RD, Wei C, Silvestre AJ. HIV risk among substance-using men who have sex with men and women (MSMW):
Findings from South Florida. AIDS Behavior, published online: 08 May 2013.
Garcia-Bates TM, Cordeiro MT, Nascimento EJ, Smith AP, Soares de Melo KM, McBurney SP, Evans JD, Marques ET Jr,
Barratt-Boyes SM. Association between magnitude of the virus-specific plasmablast response and disease severity in dengue
patients. Journal of Immunology 190:80-87, 2013.
Gingo MR, Balasubramani GK, Kingsley L, Rinaldo CR Jr, Alden CB, Detels R, Greenblatt RM, Hessol NA, Holman S, Huang L,
Kleerup EC, Phair J, Sutton SH, Seaberg EC, Margolick JB, Wisniewski SR, Morris A. The impact of HAART on the respiratory
complications of HIV infection: longitudinal trends in the MACS and WIHS cohorts. PLoS One. 8:e58812, 2013.
Gopal R, Rangel-Moreno J, Slight S, Lin Y, Nawar HF, Fallert Junecko BA, Reinhart TA, Kolls J, Randall TD, Connell TD,
Khader SA. Interleukin-17-dependent CXCL13 mediates mucosal vaccine-induced immunity against tuberculosis. Mucosal
immunology. 6:972-984, 2013
Gopal R, Monin L, Torres D, Slight S, Mehra S, McKenna KC, Fallert Junecko BA, Reinhart TA, Kolls J, Báez-Saldaña R, CruzLagunas A, Rodríguez-Reyna TS, Kumar NP, Tessier P, Roth J, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Cumming B,
Kasprowicz VO, Steyn AJ, Babu S, Kaushal D, Zúñiga J, Vogl T, Rangel-Moreno J, Khader SA. S100A8/A9 proteins mediate
neutrophilic inflammation and lung pathology during tuberculosis. Am J Respir Crit Care Med. 188:1137-1146, 2013.
Guha D, Ayyavoo V. Innate Immune Evasion Strategies by Human Immunodeficiency Virus Type 1. ISRN AIDS. 2013:954806,
2013.
Gupta P, Lackman-Smith C, Snyder B, Ratner D, Rohan L, Patton D, Ramratnam B, Cole A. Antiviral Activity of Retrocyclin RC101, a Candidate Microbicide Against Cell-Associated HIV-1. AIDS Res. Hum. Retrov. 29:391-396, 2013.
Hussain SK, Zhu W, Chang SC, Breen EC, Vendrame E, Magpantay L, Widney D, Conn D, Sehl M, Jacobson LP, Bream JH,
Wolinsky S, Rinaldo CR, Ambinder RF, Detels R, Zhang ZF, Martínez-Maza O. Serum levels of the chemokine CXCL13, genetic
variation in CXCL13 and its receptor CXCR5, and HIV-associated non-hodgkin B-cell lymphoma risk. Cancer Epidemiol
Biomarkers Prev. 22:295-307, 2013.
33
Kaabinejadian S, Piazza P, McMurtrey CP, Vernon SR, Cate J, Bardet W, Schafer B, Jackson KW, Campbell DM, Buchli R,
Rinaldo CR, Hildebrand H. Identification of Class I HLA T Cell Control Epitopes for West Nile Virus. PloS One. 8:e66298, 2013.
Kader M, Smith AP, Guiducci C, Wonderlich ER, Normolle D, Watkins SC, Barrat FJ, Barratt-Boyes SM. Blocking TLR7 and
TLR9 mediated IFN-α production by plasmacytoid dendritic cells does not diminish immune activation in early SIV infection. PLoS
Pathogens. 9:e1003530, 2013.
Lane J, McLaren PJ, Dorrell L, Shianna KV, Stemke A, Pelak K, Moore S, Oldenburg J, Alvarez-Roman MT, Angelillo-Scherrer A,
Boehlen F, Bolton-Maggs PH, Brand B, Brown D, Chiang E, Cid-Haro AR, Clotet B, Collins P, Colombo S, Dalmau J, Fogarty P,
Giangrande P, Gringeri A, Iyer R, Katsarou O, Kempton C, Kuriakose P, Lin J, Makris M, Manco-Johnson M, Tsakiris DA, MartinezPicado J, Mauser-Bunschoten E, Neff A, Oka S, Oyesiku L, Parra R, Peter-Salonen K, Powell J, Recht M, Shapiro A, Stine K, Talks
K, Telenti A, Wilde J, Yee TT, Wolinsky SM, Martinson J, Hussain SK, Bream JH, Jacobson LP, Carrington M, Goedert JJ, Haynes
BF, McMichael AJ, Goldstein DB, Fellay J, NIAID Center for HIV/AIDS Vaccine Immunology (CHAVI). A genome-wide
association study of resistance to HIV infection in highly exposed uninfected individuals with hemophilia A. Hum. Molec. Genet.
22:1903-1910, 2013.
Lucero C, Junecko B, Klamar C, Sciullo L, Berendam S, Cillo A, Qin S, Sui Y, Sanghavi S, Murphey-Corb M, Reinhart T.
Macaque Paneth Cells Express Lymphoid Chemokine CXCL13 AND Other Antimicrobial Peptides Not Previously Described As
Expressed in Intestinal Crypts. Clin. Vaccine Immunol. 20:1320-1328, 2013.
Mailliard R, Smith KN, Fecek RJ, Rappocciolo G, Nascimento E, Marques E T H, Watkins SC, Mullins JI, Rinaldo CR.
Selective Induction of CTL helper rather than killer activity by natural epitope variants promotes dendritic cell-mediated HIV-1
dissemination. J. Immunol. 191:2570-2580, 2013.
Maldarelli F, Kearney M, Palmer S, Stephens R, Mican J, Polis MA, Davey RT, Kovacs J, Shao W, Rock-Kress D, Metcalf JA, Rehm
C, Greer SE, Lucey DL, Danley K, Alter H, Mellors JW, Coffin JM. HIV populations are large and accumulate high genetic
diversity in a nonlinear fashion. J Virol. 87:10313-10323, 2013.
Meteer JD, Schinazi RF, Mellors JW, Sluis-Cremer N. Molecular mechanism of HIV-1 resistance to 3'-azido-2',3'-dideoxyguanosine.
Antiviral Res. 101C:62-67, 2013.
Nadgir SV, Hensler HR, Knowlton ER, Rinaldo CR, Rappocciolo G, Jenkins FJ. Fifty percent tissue culture infective dose assay
for determining the titer of infectious human herpesvirus 8. J. Clin. Microbiol 51:1931-1934, 2013.
Nascimento EJ, Mailliard RB, Khan AM, Sidney J, Sette A, Guzman N, Paulaitis M, de Melo AB, Cordeiro MT, Gil LV, Lemonnier
F, Rinaldo C, August JT, Marques ET Jr. Identification of conserved and HLA promiscuous DENV3 T-cell epitopes. PLoS Negl
Trop Dis. 7:e2497, 2013.
Nicholaou MJ, Martinson JJ, Abraham AG, Brown TT, Hussain SK, Wolinsky SM, Kingsley LA. HAART-Associated
Dyslipidemia Varies by Biogeographical Ancestry in the Multicenter AIDS Cohort Study. AIDS Res. Hum. Retrov. 29:871-879,
2013.
Parikh UM, Kiepiela P, Ganesh S, Gomez K, Horn S, Eskay K, Kelly C, Mensch B, Gorbach P, Soto-Torres L, Ramjee G, Mellors
JW; IPTc Taskforce. Prevalence of HIV-1 drug resistance among women screening for HIV prevention trials in KwaZulu-Natal,
South Africa (MTN-009). PLoS One. 8:e59787, 2013
Pastor AF, Rodrigues Moura L, Neto JW, Nascimento EJ, Calzavara-Silva CE, Gomes AL, Silva AM, Cordeiro MT, Braga-Neto U,
Crovella S, Gil LH, Marques ET Jr, Acioli-Santos B. Complement factor H gene (CFH) polymorphisms C-257T, G257A and
haplotypes are associated with protection against severe dengue phenotype, possible related with high CFH expression. Hum
Immunol. 74:1225-1230, 2013.
Qin S, Klamar CR, Fallert Junecko BA, Craigo J, Fuller DH, Reinhart TA. Functional characterization of ferret CCL20 and
CCR6 and identification of chemotactic inhibitors. Cytokine. 61:924-932, 2013.
Qin S, Junecko BA, Lucero CM, Klamar CR, Trichel AM, Murphey-Corb MA, Tarwater PM, Kirschner DE, Reinhart TA.
Simian immunodeficiency virus infection potently modulates chemokine networks and immune environments in hilar lymph nodes of
cynomolgus macaques. J Acquir Immune Defic Syndr. 63:428-437 2013.
Queiroz SR, Silva AN, Santos JJ, Marques ET Jr, Bertani GR, Gil LH. Construction of yellow fever virus subgenomic replicons by
yeast-based homologous recombination cloning technique. An Acad Bras Cienc. 85:159-168, 2013.
34
Rinaldo CR. HIV-1 Trans Infection of CD4+ T Cells by Professional Antigen Presenting Cells. Scientifica (Cairo). 2013:164203,
2013.
Rodriguez B, Asmuth DM, Matining RM, Spritzler J, Jacobson JM, Mailliard RB, Li XD, Martinez AI, Tenorio AR, Lori F,
Lisziewicz J, Yesmin S, Rinaldo CR, Pollard RB. Safety, tolerability, and immunogenicity of repeated doses of DermaVir, a andidate
therapeutic HIV vaccine, in HIV-infected patients receiving combination antiretroviral therapy: Results of the ACTG 5176 Trial. J.
AIDS, 64:351-359, 2013.
Rohatgi S, Gohil S, Kuniholm MH, Schultz H, Dufaud C, Armour KL, Badri S, Mailliard RB, Pirofski LA. Fc gamma receptor 3A
polymorphism and risk for HIV-associated cryptococcal disease. mBio. 4:e00573-13, 2013.
Sambandan D, Dao DN, Weinrick BC, Vilchèze C, Gurcha SS, Ojha A, Kremer L, Besra GS, Hatfull GF, Jacobs WR Jr. KetoMycolic acid-dependent pellicle formation confers tolerance to drug-sensitive mycobacterium tuberculosis. mBio. 4:e00222-13, 2013.
Schuler P, Macatangay BJC, Saze, Z, Jackson EK, Riddler SA, Buchanan WG, Hilldorfer BB, Mellors JW, Whiteside TL, Rinaldo
CR CD4+CD73+ T cells are associated with lower T cell activation and CRP levels and are depleted in HIV-1 infection regardless of
viral suppression. AIDS 27:1545-1555, 2013.
Silva MM, Gil LH, Marques ET Jr, Calzavara-Silva CE. Potential biomarkers for the clinical prognosis of severe dengue. Mem Inst
Oswaldo Cruz. 108:755-762, 2013.
Silvestre AJ, Beatty RL, Friedman MR. Substance Use Disorder in the Context of LGBT Health: A Social Work Perspective.
Social Work in Publ. Hlth. 28:366-376, 2013.
Slight SR, Rangel-Moreno J, Gopal R, Lin Y, Fallert Junecko BA, Mehra S, Selman M, Becerril-Villanueva E, Baquera-Heredia J,
Pavon L, Kaushal D, Reinhart TA, Randall TD, Khader SA. CXCR5¿ T helper cells mediate protective immunity against
tuberculosis. J. Clin. Invest. 123:712-726, 2013.
Soto-Rivera J, Patterson BK, Chen Y, Shen C, Ratner D, Ding M, Tumne A, Gupta P. Study of HIV-1 transmission across cervical
mucosa to tonsil tissue cells using an organ culture. Amer. J. Reprod. Immunol. 69:52-63, 2013.
Steckbeck JD, Sun C, Sturgeon TJ, Montelaro RC. Detailed topology mapping reveals substantial exposure of the "cytoplasmic" Cterminal tail (CTT) sequences in HIV-1 Env proteins at the cell surface. PLoS One. 8:e65220, 2013.
Taiwo B, Matining RM, Zheng L, Lederman MM, Rinaldo CR, Kim PS, Berzins BI, Kuritzkes DR, Jennings A, Eron JJ Jr, Wilson
CC. Associations of T cell activation and inflammatory biomarkers with virological response to darunavir/ritonavir plus raltegravir
therapy. J Antimicrob Chemother. 68:1857-1861, 2013.
Wasil LR, Tomaszewski MJ, Hoji A, Rowe DT. The effect of Epstein-Barr virus Latent Membrane Protein 2 expression on the
kinetics of early B cell infection. PLoS One 8:e54010, 2013.
Witt MD, Seaberg EC, Darilay A, Young S, Badri S, Rinaldo CR, Jacobson LP, Detels R, Thio CL. Incident hepatitis C virus
infection in men who have sex with men: a prospective cohort analysis, 1984-2011. Clin Infect Dis. 57:77-84, 2013.
Witt MD, Lewis RJ, Rieg G, Seaberg EC, Rinaldo CR, Thio CL. Predictors of the isolated hepatitis B core antibody pattern in HIVinfected and -uninfected men in the multicenter AIDS cohort study. Clin Infect Dis. 56:606-612, 2013.
Wonderlich ER, Wijewardana V, Liu X, Barratt-Boyes SM. Virus-encoded TLR ligands reveal divergent functional responses of
mononuclear phagocytes in pathogenic simian immunodeficiency virus infection. Journal of Immunology. 190:2188-2198, 2013.
Wonderlich ER, Barratt-Boyes SM. SIV infection of rhesus macaques differentially impacts mononuclear phagocyte responses to
virus-derived TLR agonists. J Med Primatol. 42:247-253, 2013.
Xavier Eurico de Alencar L, de Mendonça Braga-Neto U, José Moura do Nascimento E, Tenório Cordeiro M, Maria Silva A,
Alexandre Antunes de Brito C, da Silva Mda P, Gil LH, Montenegro SM, Marques ET Jr. HLA-B∗44 Is Associated with Dengue
Severity Caused by DENV-3 in a Brazilian Population. J Trop Med. 2013:648475, 2013.
Yang Y, Bhati A, Ke D, Gonzalez-Juarrero M, Lenaerts A, Kremer L, Guerardel Y, Zhang P, Ojha AK. Exposure to a cutinase-like
serine esterase triggers rapid lysis of multiple mycobacterial species. J. Biolog. Chem. 288:382-392, 2013.
Zych C, Domling A, Ayyavoo V. Development of a robust cell-based high-throughput screening assay to identify targets of HIV-1
viral protein R dimerization. Drug Des Devel Ther. 7:403-412, 2013.
35
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