Absolute Certainty! - Genesee Scientific

Eppendorf Consumables – it’s Your sample
Absolute Certainty!
Quality – Purity – Reliability for Your Applications
The Eppendorf Quality · Eppendorf consumables
The Eppendorf Quality · Eppendorf manufacturing standards
Experience the Eppendorf Quality™
Don’t Leave Your Results to Chance
Eppendorf consumables
Providing excellence for scientific experts
Eppendorf invented the microtube and created a
standard that to the present day is used in all research
and diagnostic laboratories throughout the world.
During the last 5 decades Eppendorf has become one
of the world´s leading manufacturers of highest quality
consumables. The outstanding quality of Eppendorf
consumables is a result of experience and constant
development – with your research needs in mind.
Eppendorf manufacturing standards
In touch with your ambitions
In 1962 the legendary “Eppi” tube with 1.5 mL started
a series of innovative consumable products that
have improved workflows and experimental results in
many applicational areas. Our main objective always
has been to guarantee highest reliability and product
consistency for your experiments.
Only the best is good enough
Eppendorf consumables are produced, handled,
stored and delivered according to highest quality and
purity standards – that is what our customers
call the Eppendorf Quality. Our consumables are
manufactured from carefully selected and purest
raw materials that comply with international criteria
for maximum purity.
Fully automated production in clean room
conditions excludes human interaction and possible
contamination.
Just examples of our quality process. With the
strictest control criteria, internally and externally
monitored, we guarantee the superior consistency
of our products and your results – batch by batch.
For peace of mind and best research results.
Count on Eppendorf!
Learn more about Eppendorf Consumables:
www.eppendorf.com/consumables
Dr. Andrew Holt
Department of Pharmacology,
University of Alberta, Canada
“We have seen substantial inhibition of our
enzyme assays by chemicals leaching from
disposable plastic consumables. To obtain the
best possible reproducibility we use consumables
from manufacturers that can confirm the absence
of critical manufacturing additives.”
Karen Thomsen,
Microbiology Central Laboratory,
Hamburg Water GmbH, Germany
““We need to avoid that contaminants from the
plastic material enter the sample and inhibit
bacterial growth. The consumables that we use
to analyse water samples should be of the highest
purity to obtain reliable results.”
Dr. Paul Gooding,
Plant Genomics Centre, Australian Genome Research Facility
“Our DNA isolation protocols from both animal and plant
material require grinding of tissue prior to and during the
cell lysis process. As we work with high numbers of samples,
breaking of consumables and subsequent sample loss
can be critical. The excellent quality and stability of the
1 ml Deepwell Plates from Eppendorf convinced us as it improved
the reliability of our process significantly.”
2
3
The Eppendorf Quality · Eppendorf purity grades
A System You Can Rely On
Eppendorf purity grades
Tailored to your application needs
Eppendorf has set industry standards in consumable
purity levels. Building up on the famous Eppendorf
Quality, four additional purity grades specialize
Eppendorf’s Consumables for various applicational
needs – Biopur, PCR clean, Sterile, and Protein free.
Paving the way for modern lab standards
In addition to our strict internal process controls,
Protein-free, Sterile, PCR clean and Biopur
products are tested by an independent, external
analytical laboratory for compliance – lot by lot.
The results of these testings are certified and
can be downloaded from
www.eppendorf.com/certificates
You have highest expectations on consistency
and reproducibility?
Make Eppendorf consumables your choice!
Biopur
Continuous,
lot-specific quality controls
Certified:
‡ Human DNA-free
‡ Bacterial DNA-free
‡ DNase-free – NEW!
‡ RNase-free
‡ PCR inhibitor-free – NEW!
Sterile
Continuous,
lot-specific quality controls
‡ ATP-free
‡ Pyrogen-free
‡ Sterile
Example applications:
PCR clean
Continuous,
lot-specific quality controls
For highest purity demands in cell culture,
nucleic acid analytics, diagnostics
or hygiene monitoring
Certified:
Certified:
‡ Human DNA-free
‡ Pyrogen-free – NEW!
‡ DNase-free
‡ Sterile
Eppendorf
Quality
Continuous quality controls
‡ RNase-free
Protein-free
Continuous,
lot-specific quality controls
‡ PCR inhibitor-free
Example applications:
Example applications:
Microbiology and
The perfect choice for isolation, storage and
cell culture applications
analytics (PCR/qPCR, microarrays)
of nucleic acids
Certified:
‡ Protein-free
4
Example applications:
Example applications:
All routine lab applications
Measurement of protein samples
5
The Eppendorf Quality · Minimizing the risk of chemical leaching from consumables
The Eppendorf Quality · Unaffected assay results
Get Peace of Mind!
Minimizing the risk of chemical leaching from consumables
Not all contamination is biological
The reliability and consistency of your research results
are our priority when developing new consumables.
Chemicals used in the manufacturing of disposable
plastic labware, such as slip agents, plasticizers or
biocides, can leach out of the plastic into your samples.
Recent scientific reports have shown that these
chemicals can be carried over to all of your downstream applications leading to erroneous results.1–5
UV absorbing substances from plastic vials can leach
into the sample, especially after heating in e.g. PCR,
centrifugation, ultrasound, lysis and homogenization3.
These substance interfere with photometric detection
reactions, thus providing a source of error in downstream applications.
Make the right choice for better results
To ensure highest reliability and consistency for your
experiments, Eppendorf has optimized materials
and processes to minimize the risk of interference:
‡ Eppendorf Plates, epT.I.P.S. pipette tips and
Eppendorf Tubes are made of highest quality,
virgin polypropylene (PP) free of biocides,
plasticizers and latex
‡ Optimized, highly polished molds produce
without the use of slip agents like oleamide,
erucamide, and stearamide
‡ The dyes used for Eppendorf Plates are free
of organic additives and heavy metals
‡ Clear information and certification on additives
and trace metals are available
‡ Figure 1: Chemicals released from different brands of tubes can
‡ Figure 2: The brand of tube can affect evaporation rate in
contaminate your sample. As described in a recent publication3
vacuum concentration. Chemicals, for example oily slip agents,
these chemicals skew absorbance readings and lead to erroneous
released from the tube plastic can slow down evaporation.
DNA quantification (in this example up to 16 µg/mL).
Some of these slip agents (e.g., oleamide) have also been shown
to negatively affect the outcome of biological tests like enzyme
activity or receptor-binding assays.1,2,5
3.0
2.5
0.9
0.8
0.7
Evaporation (g)
Absorbance
2.0
1.5
1.0
260 nm
0.6
0.5
0.4
0.3
0.2
0.5
0.1
0.0
0.0
220
245
270
295
320
345
370
Eppendorf Eppendorf
Safe-Lock395
3810X
A
B
C
D
M
R
S
T
U
V
Wavelength (nm)
 Eppendorf Safe-Lock Tubes
 Eppendorf Tubes 3810X
Brand A  B  C  D  M 
R S T  U V 
 Eppendorf Safe-Lock Tubes
 Eppendorf Tubes 3810X
Brand A  B  C  D  M 
R S T  U V 
References:
1. McDonald G. R. et al.: Science, 322, 917 (2008)
2. Reid G. et al.: GIT Laboratory Journal, 9–10, 2–4 (2009)
3. Lewis, L. K. et al.: BioTechniques, 48, 297-302 (2010)
So ask yourself:
is it really DNA you are measuring?
Visit the lab
of Science author Andy Holt.
www.eppendorf.com/lab
4. Belaiche C. et al.: Clin. Chem., 55, 1883-1884 (2009)
5. Watson J. et al.: J Biomol Screen, 14(5), 566–572 (2009)
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Eppendorf Consumables · LoBind Tubes and Plates
Eppendorf Consumables · LoBind Tubes and Plates
Eppendorf LoBind® Consumables
Now You Have It All!
LoBind makes the difference
Typical protein recovery rates in
Eppendorf Protein LoBind consumables
Product features and benefits
‡ 2 component LoBind polymer material guarantees
maximum sample recovery
‡ Free of surface coating (e. g. siliconization).
‡ Available in tube and plate formats for easy up-scaling
‡ High stability for use in all sample preparation protocols
‡ PCR clean quality batch tested and certified
material. The Eppendorf Protein LoBind material guarantees up to
97 % sample recovery independent on incubation time. In contrast,
protein sticks to standard material resulting in nearly 50 % or 90 %
80 %
‡ DNA LoBind for DNA or RNA applications in forensic analysis,
‡ Protein LoBind for protein, peptide, antibody or virus samples
Protein LoBind Deepwell Plates and plates made of standard
90 %
Eliminates the risk of sample interference
microarrays, next generation sequencing
Fig. 1: Protein recovery (fluorescein-labeled BSA) in Eppendorf
100 %
Recovery rate [%]
When biological samples are stored or incubated in
standard reaction vessels more than 90 % sample
material can be lost within 24 hours due to adsorption
to the plastic surface. Eppendorf LoBind Tubes and
Plates maximize sample recovery by significantly
reducing sample-to-surface binding – without the use
of any type of coating!
Protection from sample loss for improved assay results
sample loss after 3 h or 24 h, respectively.
70 %
60 %
50 %
40 %
30 %
20 %
10 %
0%
0
1
3
Incubation time [h]
Eppendorf Protein LoBind
24
Standard Material
Ordering information
Description
Eppendorf Protein LoBind consumables, PCR clean
Quantity
Safe-Lock Tube 0.5 mL
Safe-Lock Tube 1.5 mL
Safe-Lock Tube 2.0 mL
100 tubes (2 bags of 50)
100 tubes (2 bags of 50)
100 tubes (2 bags of 50)
0030 108.094
0030 108.116
0030 108.132
Deepwell Plate1 96/1000 µL
Deepwell Plate1 96/500 µL
Deepwell Plate1 384/200 µL
Deepwell Plate1 96/2000 µL
20 plates (5 bags of 4)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
20 plates (5 bags of 4)
0030 504.208
0030 504.100
0030 524.101
0030 504.305
Microplate 384/120 µL (V-bottom)
Eppendorf DNA LoBind consumables, PCR clean
80 plates (5 bags of 16)
0030 624.300
Safe-Lock Tube 0.5 mL
Safe-Lock Tube 1.5 mL
Safe-Lock Tube 2.0 mL
250 tubes (5 bags of 50)
250 tubes (5 bags of 50)
250 tubes (5 bags of 50)
0030 108.035
0030 108.051
0030 108.078
Deepwell Plate1 96/1000 µL
Deepwell Plate1 96/500 µL
Deepwell Plate1 384/200 µL
20 plates (5 bags of 4)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
0030 503.201
0030 503.104
0030 523.105
Microplate 384/120 µL (V-bottom)
Microplate 96/300 µL (V-bottom)
80 plates (5 bags of 16)
80 plates (5 bags of 16)
0030 623.304
0030 603.303
1
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5
Order no.
Plates with clear wells and white OptiTrack® border color. For more information and other border colors please contact your local Eppendorf distributor or visit www.eppendorf.com/consumables
9
Eppendorf Consumables · ep Dualfilter T.I.P.S.
Eppendorf Consumables · ep Dualfilter T.I.P.S.
ep Dualfilter T.I.P.S.® pipette tips
Contamination: Locked Out
Two filter layers are better than one
Dual protection against contamination for pipette and
sample is provided by the ep Dualfilter T.I.P.S. filter tips.
This unique two phase filter retains nearly all aerosols
and biomolecules, achieved by well-defined, different
pore sizes in the two filter layers.
‡ Without a filter
, the pipette is exposed to
contamination by samples and aerosols.
Conventional single-layer filters
do not
The difference between filter tips and filter tips
Aerosol/particle penetration of various filter tips*
Particle permeability
Pipette
ep Dualfilter T.I.P.S.
Pipette
cone
Filter
fully block particles and molecules.
Only ep Dualfilter T.I.P.S
provide reliable
protection even against the finest impurities.
DNA permeability
Pipette
tip
0,13% ±0.11%
0.13%
±0,11%
0,02% ±0.01%
0.02%
±0,01%
Filter tips
Manufacturer A
15,00% ±5.40%
15.00%
±5,40%
Filter tips
Manufacturer B
88,00% ±7.00%
88.00%
±7,00%
Filter tips
Manufacturer C
9,70% ±2.60%
9.70%
±2,60%
Filter tips
Manufacturer D
15,00% ±4.00%
15.00%
±4,00%
Filter tips
Manufacturer E
7,20% ±1.70%
7.20%
±1,70%
0,43% ±0.03%
0.43%
±0,03%
12,00% ±5.20%
12.00%
±5,20%
0,87% ±0.40%
0.87%
±0,40%
0,96% ±0.11%
0.96%
±0,11%
1,40% ±0.39%
1.40%
±0,39%
* Data generated by
Order information
Description
ep Dualfilter T.I.P.S.® Racks, PCR Clean, Sterile
10
Product Features
Your Benefits
‡ Two-phase filter
– ultimate protection against contamination of sample and pipette
‡ Sterile
– ready-to-use and time-saving
– no need to sterilize the pipette tips
‡ PCR clean
– free of human DNA, DNase and RNase
– free of PCR inhibitors (contact of liquid with filter material
does not influence PCR)
‡ Free of pyrogen (endotoxin)
– applicable in drug research, cell culture and medical laboratories
‡ Hydrophobic Material
– no absorption of aqueous solutions into the filter
– sample is not absorbed and not lost in the filter
– no sample loss due to false pipetting
Quantity
Order no.
0.1–10 µL S
0.1–10 µL M
0.5–20 µL L
2–20 µL
2–100 µL
2–200 µL
20–300 µL
50–1,000 µL
ep Dualfilter T.I.P.S.® Racks, PCR Clean, Sterile
10 × 96 = 960 tips
10 × 96 = 960 tips
10 × 96 = 960 tips
10 × 96 = 960 tips
10 × 96 = 960 tips
10 × 96 = 960 tips
10 × 96 = 960 tips
10 × 96 = 960 tips
0030 077.504
0030 077.512
0030 077.520
0030 077.539
0030 077.547
0030 077.555
0030 077.563
0030 077.571
50 μL– 1,250 μL L
100 – 5,000 μL
1–10 mL L, individually blistered
ep Dualfilter T.I.P.S.® LoRetention Racks, PCR Clean, Sterile
5 × 96 = 480 tips
5 × 96 = 480 tips
50 pieces per package
0030 077.750
0030 077.580
0030 077.598
0.1–10 μL S
0.5–20 μL L
2–100 μL
20–300 μL
50–1,000 μL
10 × 96 = 960 tips
10 × 96 = 960 tips
10 × 96 = 960 tips
10 × 96 = 960 tips
10 × 96 = 960 tips
0030 077.610
0030 077.628
0030 077.644
0030 077.636
0030 077.652
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Eppendorf Consumables · epT.I.P.S. LoRetention
Eppendorf Consumables · epT.I.P.S. LoRetention
epT.I.P.S.® LoRetention
The Pearl Effect!
Increased sensitivity with maximum reproducibility
Don’t leave valuable samples in your tips when
pipetting solutions that contain detergents! Based on
a unique and innovative method of molecular material
modification, the new Eppendorf epT.I.P.S. LoRetention
pipette tips allow you to transfer almost 100 % of the
liquid picked up (“pearl effect”).
Product features
‡ Ultrahomogeneous surface for maximum reproducibility
‡ Minimum sample loss thanks to ultrahydrophobic surface
Compare it yourself
epT.I.P.S. LoRetention perform considerably better
in terms of precision and sample recovery than
low-retention tips from other manufacturers.*
‡ Significantly reduced build up of foam when pipetting
‡ Autoclavable and chemical resistant
‡ Available in PCR clean and Eppendorf Quality
‡ Available as ep Dualfilter T.I.P.S. in PCR clean and sterile
purity grades
‡ Comparison of residual liquid from epT.I.P.S. LoRetention with
standard epT.I.P.S. pipette tips and low-retention tips of other
PCR master mix and the detergent 0.1 % Triton X-100. Once the
residual liquid when pipetting solutions that contain
detergents:
Maximum recovery with epT.I.P.S. LoRetention
Significant loss of sample using standard tips.
determined. The measurements were repeated several times
and the standard deviation determined. epT.I.P.S. LoRetention
pipette tips resulted in the lowest residual liquid, as well as the
smallest standard deviation.
PCR-Mastermix
5,0
Residual liquid [µl]
liquid had been dispensed, the residual liquid in the tips was
‡ The “Pearl Effect” – a comparison of the amount of
0,1% Triton X-100
6,0
manufacturers. 200 µL tips were used to pipette a commercial
4,0
3,0
2,0
1,0
0,0
epT.I.P.S.
epT.I.P.S
LoRetention
Competitor A
Low-Retention
Competitor M
Low-Retention
Competitor S
Low-Retention
Ordering Information
2
1
Description
ep Dualfilter T.I.P.S.® LoRetention, Racks, PCR clean, Sterile
Quantity
Order No.
0.1–10 µL S
0.5–20 µL L
2–100 µL
20–300 µL
50–1,000 µL
epT.I.P.S.® LoRetention, sets, Eppendorf Quality
10 × 96 tips
10 × 96 tips
10 × 96 tips
10 × 96 tips
10 × 96 tips
0030 077.610
0030 077.628
0030 077.644
0030 077.636
0030 077.652
0.1–10 µL S
0.5–20 µL L
2–200 µL
50–1,000 µL
epT.I.P.S.® LoRetention, Reloads, Eppendorf Quality
5 × 96 tips + 1 box
5 × 96 tips + 1 box
5 × 96 tips + 1 box
5 × 96 tips + 1 box
0030 072.251
0030 072.260
0030 072.278
0030 072.286
0.1–10 µL S
0.5–20 µL L
2–200 µL
50–1,000 µL
epT.I.P.S.® LoRetention, Reloads, PCR clean
10 × 96 tips
10 × 96 tips
10 × 96 tips
10 × 96 tips
0030 072.049
0030 072.057
0030 072.065
0030 072.073
0.1–10 µL S
0.5–20 µL L
2–200 µL
50–1,000 µL
10 × 96 tips
10 × 96 tips
10 × 96 tips
10 × 96 tips
0030 072.006
0030 072.014
0030 072.022
0030 072.030
* Data and more information in the Application Notes 218 and 192 on www.eppendorf.com/consumables
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Eppendorf Consumables · twin.tec PCR Plates
Eppendorf Consumables · twin.tec PCR Plates
Eppendorf twin.tec® PCR Plates
No Oddities!
Just an even plate
A snug fit to the PCR cycler and a solid base for
tight sealing are the most important attributes of
a PCR plate. The Eppendorf twin.tec PCR plate is
dimensionally stable to support both functions.
The rigid polycarbonate frame keeps the shape
even at high temperatures. So you can take your
samples out of the plate after the PCR easily.
Choose from various designs and colors
If you think a PCR plate is just a PCR plate,
you will be surprised to see how much the twin.tec
PCR Plate from Eppendorf can offer you:
‡ Extremely thin-walled polypropylene wells for optimal
heat transfer to the sample
‡ Robust polycarbonate frames for ultimate rigidity
and torque-resistance
The effective sealing is further supported by raised
rims which will form a very tight entity with the sealing
material.
‡ Raised well rims for effective sealing, also reduces
the risk of cross-contamination
‡ Autoclavable (121°C, 20 min)
‡ Certified PCR clean
For more information, please visit:
www.eppendorf.com/pcrplates
‡ Also available with individual barcode
(not for unskirted plates)
Ordering information
Description
twin.tec® PCR Plate 96, skirted, PCR clean
Quantity
Order no.
‡ Clear
‡ Yellow
‡ Green
‡ Blue
‡ Red
twin.tec® PCR Plate 96, semi-skirted, PCR clean
25 plates
(5 bags of 5)
0030 128.648
0030 128.656
0030 128.664
0030 128.672
0030 128.680
‡ Clear
‡ Yellow
‡ Green
‡ Blue
‡ Red
twin.tec® PCR Plate 96, unskirted low profile, PCR clean
25 plates
(5 bags of 5)
0030 128.575
0030 128.583
0030 128.591
0030 128.605
0030 128.613
‡ Clear
‡ Yellow
‡ Green
‡ Blue
‡ Red
‡ Clear (divisible)
‡ Blue (divisible)
twin.tec® PCR Plate 96, unskirted (250 µL), PCR clean
20 plates
(4 bags of 5)
0030 133.307
0030 133.315
0030 133.323
0030 133.331
0030 133.340
0030 133.358
0030 133.382
‡ Clear
‡ Blue
‡ Clear (divisible)
‡ Blue (divisible)
20 plates
(4 bags of 5)
0030 133.366
0030 133.390
0030 133.374
0030 133.404
10 plates
(individually blistered)
0030 129.300
0030 129.318
10 plates
(individually blistered)
0030 129.326
0030 129.334
10 plates
(individually blistered)
0030 129.342
0030 129.350
25 plates
(5 bags of 5)
0030 128.508
0030 128.516
0030 128.524
0030 128.532
0030 128.540
twin.tec® microbiology PCR Plate 96, skirted, Biopur®
‡ Clear
‡ Blue
twin.tec® microbiology PCR Plate 96, semi-skirted, Biopur®
‡ Clear
‡ Blue
twin.tec® microbiology PCR Plate 384, Biopur®
‡ Clear
‡ Blue
twin.tec® PCR Plate 384, PCR clean
‡ Clear
‡ Yellow
‡ Green
‡ Blue
‡ Red
127,76 ±0,2
99 ±0,15
14
85,47 ±0,15
11,24
14,38
15
Eppendorf Consumables – it’s Your sample
Find more literature, movies, relaxing games and your personal consumables navigator on
www.eppendorf.com/consumables
Your local distributor: www.eppendorf.com/worldwide
Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556 · E-mail: [email protected]
Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA
Tel: +1 516 334 7500 · Toll free phone: +1 800 645 3050 · Fax: +1 516 334 7506 · E-mail: [email protected]
Application Support Europe: Tel: +49 1803 666 789
(Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min) [email protected]
North America: Tel: +1 800 645 3050 · E-mail: [email protected]
Asia Pacific: Tel: +60 3 8023 6869 · E-mail: [email protected]
eppendorf®, Eppendorf Plates®, Eppendorf Tubes®, Eppendorf twin.tec®, Eppendorf LoBind®, Biopur®, OptiTrack®, epT.I.P.S.® and ep Dualfilter T.I.P.S.® are registered trademarks and epT.I.P.S. LoRetention, Eppendorf Quality™,
Eppi™ and Eppendorf Consumables™ are trademarks of Eppendorf AG. All rights reserved, including graphics and images. Copyright © 2011 by Eppendorf AG. Technical specifications subject to change.
Order No. A003 012 020/GB6/5T/1111/FEEL/STEF · Printed climate neutrally in Germany
Eppendorf Consumables™ –
Absolute Certainty!