Professional Resume - The University of Kansas

Transcription

CURRICULUM VITAE
1. Personal information.
Name: ANTONIO ARTIGUES SERRA.
Office address:
3901 Rainbow Blvd.
1058 HLSIC, MS 3030
Kansas City, KS, 66160
Phone: (913) 588 3487
E-mail: [email protected]
Home Address:
729 W 121 st street
Kansas City, MO 64145
Phone: (816) 941 7403
[email protected]
2. Education.
Ph.D. in Biochemistry, School of Sciences, University of the Balearic Islands.
1987.
Pharmacy graduate and Master in Pharmacy in Pharmacy, School of Pharmacy,
University of Navarra. 1982.
Biology graduate and Master in Biology, School of Sciences, University of
Navarra. 1979.
3. Academic appointments or other significant work experience.
2004 – present Research Associate Professor. Director of the Mass
Spectrometry Laboratory. Department of Biochemistry and
Molecular Biology. School of Medicine. University of Kansas
Medical Center. KS
2001 - 2004
Research Assistant Professor and Deputy Director of Research
and Development. Mass Spectometry Facility. School of Biological
Sciences, University of Missouri-Kansas City.
1996 - 2001
Supervisor of Macromolecular Analysis Core Facilities. School of
Biological Sciences. University of Missouri-Kansas City. MO
1992-1996
Research instructor, Department of Molecular Biology and
Biochemistry, University of Missouri-Kansas City. MO
1991
Fellow, Neurochemistry
University of Alicante.
1988-1990
postdoctoral researcher, Department of Biochemistry and
Molecular Biophysics, Medical College of Virginia, Virginia
Commonwealth University, Richmond, Virginia, USA.
Department,
School
of
Medicine,
4. Teaching assignments.
2009:
Organized the second workshop on Mass spectrometry based
proteomics. Including the participation of Dr. O. Nadeau (KU
Medical Center), D. Miller and
J. Rogers (thermoFinnigan
Institute)
Facilitator in the small group discussions (Huntington Disease) of
the Genetics and Neoplasia (core 804)
2008:
IGPBS Module 2: Mass spectrometry in proteomics. Application of
mass spectrometry to systems biology (6 impact hours)
Facilitator Small group discussions Huntington Disease (4 impact
hour)
2007
IGPBS Module 4: Introduction
spectrometry (4 impact hours)
2006
Foundations of medicine module 1. Author on Diagnostic
applications of proteomics. Group leader on Diagnostic
applications of proteomics (4 impact hours) and Mitochondrial
DNA and Disease ( 4 impact hours)
2005
IGPBS Module
spectrometry.
1:
Introduction
to
to
proteomics
proteomics
and
and
mass
mass
Facilitator in the Problem based learning of the course BIOC 801 Medical Biochemistry (Academic Year '06)
2004
IGPBS Module 1: Thermodynamics, protein structure and analysis
of reaction/binding kinetics
2
1996
Peptide and protein analysis by mass spectrometry (LS MBB 565
structure and function of proteins).
1993-1994:
Practical lessons of FT-IR in the course # Chem. 437 (FT-IR),
Department of Chemistry, University of Missouri-Kansas City.
5. Awards.
1991:
Fellow, Neurochemistry
University of Alicante..
Department,
School
of
Medicine,
1990:
Honorific appointment at the Neurochemistry Department, School
of Medicine, University of Alicante.
1987:
Cum Laude, Ph. D. Degree. University of the Balearic Islands.
1983:
Grant-in-aid of the "Comisión de Obras Sociales de SA NOSTRA".
1977-1978:
Fellowship of the University of Navarra.
6. Training and Certificates
2000:
Voyager Training Class. PE Biosystems. Foster City, CA.
1996:
LCQ Operations Training Course. Finnigan Institute.
1989:
Pharmacist specialist in clinical analysis.
1987:
Health Diplomate. Pamplona.
7. Membership-Scientific, honorary and Professional Societies.
1995 - to date: Member of the American Chemical Society.
1994 - to date: Member of the Spanish Society of Biochemistry
1993 - to date: Visiting Faculty of the Neurochemistry Department, School of
Medicine, University of Alicante.
1993 - to date: Member of the Spanish Society of Biophysics
1992 - to date: Member of the American Association for the Advancement of
Science.
8. Technical experience
•
Mass Spectrometry/Proteomics
•
Biological spectroscopy: Circular dichroism, Fluorescence and
Fluorescence polarization, Fourier Infrared Transform Spectroscopy (FTIR), Stopped-flow
3
•
•
•
•
•
•
Protein
purification:
ultracentrifugation,
Conventional
Liquid
Chromatography,
HPLC,
FPLC,
Affinity
chromatography,
Isoelectricfocusing, SDS-PAGE, 2D-PAGE
Protein mapping and peptide sequencing.
Mass Spectroscopy. Proteomics.
Differential Scanning Calorimetry
Analytical ultracentrifugation
Immunonological techniques: Antibodies production, Western Blot,
ELISA, RIA.
9. Academic Activities
Serving on Mary Ashley Rimmer Thesis Committee
10. Publications
27 Miller D.E.,Prasannan, C.B., Fenton, A.W, and Artigues, A. HDX Finder:
automated analysis and datareproting of deuterium/hydrogen exchange mass
spectrometry. J. Am. Soc. Mass Spectrom. 2Epub 2011 Nov 15
26 Saito, H-M Yan, A. Artigues, M.T. Villar, A. Farhood and H. Jaeschke (2010):
Mechanism of protection by metallothionein against acetaminophen
hepatotoxicity. Toxicol Appl Pharmacol. 242, 182-190. PMCID: PMC2789886.
25. Maria T. Villar, Danny E. Miller, Aron W. Fenton and Antonio Artigues (2010)
SAIDE: A Semi-Automated Interface for Hydrogen/Deuterium Exchange
Mass Spectrometry Proteomica 6, 63-69
24 Cooper AJ, Krasnikov BF, Niatsetskaya ZV, Pinto JT, Callery PS, Villar MT,
Artigues A, Bruschi SA. (2010): Cysteine S-conjugate beta-lyases: important
roles in the metabolism of naturally occurring sulfur and selenium-containing
compounds, xenobiotics and anticancer agents. Amino Acids. PMCID:
PMC2898922.
23. C. Saito, H-M Yang, A. Artigues, M.T. Villar, A. Farhood and H. Jaeshke:
Mechanism of protection by metallothionein agains acetaminophen
hepatoxicity. Toxicol Appl Pharmacol. 2010 Oct 14. [Epub ahead of print]
22. Boulatnikov, I.G., Nadeau, O.W., Daniels, P.J., Sage, J.M., Jeyasingham,
M.D., Villar, M.T., Artigues, A. and Carlson, G.M. (2008). The regulatory beta
subunit of phosphorylase kinase interacts with glyceraldehyde-3-phosphate
dehydrogenase. Biochemistry 47, 7228-7236.
21. O.W. Nadeau OW, G.J. Wyckoff, J.E. Paschall, A. Artigues, J. Sage, M.T.
Villar, and G.M. Carlson: CrossSearch, a user-friendly search engine for
detecting chemically cross-linked peptides in conjugated proteins. Mol Cell
Proteomics. 2008 Feb 16.
20. H.H-T Hsu, A. Artigues, and M.T. Villar : Induction of calcification by serum
depletion in cell culture: a model for focal calcification in aortas related to
atherosclerosis. Lipids Health Dis. 2008 Jan 29;7:2.
4
19. M. D. Jeyasingham, A. Artigues, O. W. Nadeau, and G.M. Carlson: Coevolution of the regulation of contraction and energy production in Skeletal
Muscle. J Mol Biol. 2008 Mar 28;377(3):623-9. Epub 2008 Jan 5.
19. O.W. Nadeau, D.W. Anderson, Q. Yang, A. Artigues, J.E. Paschall, G.J.
Wyckoff, J.M. McClintock and G.M. Carlson: Evidence for the Location of the
Allosteric Activation Switch in the Multisubunit phosphorylase kinase complex
from mass spectrometric identification of chemically crosslinked
peptides.J.Mol. Biol. 2007, 365: 1429-1445
18. A. Artigues, A. Iriarte, and M. Marino Martinez-Carrion: Identification of hsc70
binding sites in mitochondrial aspartate aminotransferase. Archives of
Biochemistry and Biophysiscs. 2006, 450(1):30-8.
17. J. A. Oses-Prieto, M.T. Bengoechea-Alonso, A. Arftigues, A. Iriarte, and M.
Martinez-Carrion: On the nature of the rate-limiting steps in the refolding of
the cofactor-dependent protein aspartate aminotransferase. J. Biol. Chem.
278: 49988-4999 (2003)
16. A. Artigues, A. Iriarte and M. Martinez-Carrion: Binding to Chaperones allows
import of a purified mitochondrial precursor into mitochondria. J. Biol. Chem.
277: 25047-25055 (2002).
15. J. P. Gorski, F.-T., L., A. Artigues, L.F. Castagna, and P. Osdoby: New
Alternatively Spliced Form of Galectin-3, a Member of α -Galactoside-Binding
Animal Lectin Family, Contains Predicted Transmembrane Spanning Domain
and Leucine Zipper Motif J. Biol. Chem. 277: 18840-18848 (2002).
14. A. Artigues M. Bengoechea-Alonso, D.L. Crawford, A. Iriarte, and MartinezCarrion, M: Biological implications of the different Hsp 70 binding properties
of mitochondrial and cytosolic aspartate aminotransferase In Biochemistry
and Molecular Biology of vitamin B 6 and PQQ-dependent proteins. A.Iriarte,
H.M. Kagan H.M. and M. Martinez-Carrion
(eds.) Birkäuser Verlag
Basel/Switzerland pp 111-116 (2000)
13. A. Artigues, D. L. Crawford, A. Iriarte and M. Martinez-Carrion: Divergent Hsc
70-binding properties of mitochondrial and cytosolic
aspartate
aminotransferase. Implications for their segregation to different cellular
compartments. J. Biol. Chem. 273:33130-33134 (1998)
12. F. Donate, A. Artigues, A. Iriarte, and M. Martinez-Carrion: Opposite behavior
of two isozymes when refolding in the presence of non-ionic detergents.
Protein Science 7:1911-1820 (1998)
11. C. Torella, J. R. Mattingly, Jr., A. Artigues, A. Iriarte, and M. Martinez-Carrion:
Insight into the conformation of folding intermediates of a protein trapped by
GroEL. J. Biol. Chem. 273:3915-3925 (1998)
10. A. Artigues, A. Iriarte and Martinez-Carrion: Mapping the hsp 70 binding sites
on a large mitochondrial precursor protein. In Techniques in Protein
Chemistry VIII, D.R. Marshak (editor). Academic Press, pp 481-492 (1997).
9. A. Artigues, A. Iriarte and M. Martinez-Carrion: Refolding intermediates of
acid-unfolded mitochondrial aspartate aminotransferase bind to Hsc70. J.
Biol. Chem. 272:16852-1861 (1997).
5
8. J. P. Gorsky, E. Kremer, J. Ruiz-Perez, G. E. Wise, and A. Artigues:
Conformational analyses on soluble surface bound osteopontin. Ann N.Y.
Acad Sci USA (1995) 760:12-23.
7. A. Artigues, A. Iriarte and M. Martinez-Carrion: Acid-induced reversible
unfolding of mitochondrial aspartate aminotransferase. J. Biol. Chem. 269,
21990-21999 (1994).
6. A. Iriarte, A. Artigues, B. Lain, J. R. Mattingly, Jr. and M. Martinez-Carrion:
Cytosolic factors and the twisting path from birth to berth in aspartate
aminotransferases. In Biochemistry of Vitamin B6. G. Marino, G. Sannia and
F. Bossa (eds.) Birkäuser Verlag Basel/Switzerland, pp 75-80 (1994).
5. A. Artigues, H. Farrant and V. Schirch: Role of the amino terminal region of
serine hydroxymethyltransferase in determining its in vivo rate of turnover. J.
Biol. Chem. 268, 13784-13790 (1993).
4. A. Artigues, A. Birkett and S. Verne: Evidence for the in vivo deamidation and
isomerization
of
an
asparaginyl
residue
in
cytosolic
serine
hydroxymethyltransferase. J. Biol. Chem. 265, 4853-4858 (1990).
3. A. Artigues, M. T. Villar, A. Fernandez, J. A. Ferragut, and J. M.
Gonzlez-Ros: Cholesterol modulates structural features of the nicotinic
acetylcholine receptor in model reconstituted vesicles. Biochem. Biochim.
Acta 985, 325-330 (1989).
2. M. T. Villar, A. Artigues, J. A. Ferragut and J. M. González-Ros:
Phospholipase A2 hydrolysis products as membrane specific perturbants to
probe structural features of nicotinic acetylcholine receptor. Biochem.
Biochim. Acta. 938, 35-43 (1988).
1. A. Artigues, M. T. Villar, J. A. Ferragut and J. M. González-Ros: Thermal
perturbation studies of membrane-bound acetylcholine receptor from
Torpedo: Effects of cholinergic ligands and membrane perturbants. Arch.
Biochem. Biopsy. 258, 33-41 (1987).
9.2 Invited conferences/lectures
2009: Emerging Trends presentation: Proteomics: Keys to Treatment, Cure &
Beyond. School of Nursing, KU Medical Center.
2008: Protein structure and function by mass spectrometry. Centre de Regulacio
Genomica. Barcelona, Spain
2007: Teaching workshop on protein mass spectrometry: Mass spectrometry
studies on protein folding. University of Kansas. Lawrence, Kansas.
2006: Aplicaciones de la Espectrometria de Masas al Estudio de las
Interacciones Proteina-Proteina, University of Alicante, Spain.
6
2005: “Mass Spectrometry: a tool for protein dynamics”. Facultat de Veterinaria,
Universitat Autonoma de Barcelona, Spain. May 2, 2005
2001: Regional Biophysics Collaboration: “Mass Spectrometry and Proteomics”.
School of Biological Sciences, University of Missouri-Kansas City. February 22,
2001
1999: 10th international symposium on vitamin B 6 and carbonyl catalysis and 4th
meeting on PQQ and quinoproteins: “Divergent HSP70 Binding Properties of
Two Aspartate Aminotransferase Isozymes: Biological Implications.” Santa Fe,
New Mexico, USA. October 31-November 5, 1999.
9.3 Communications (posters)
Hydrogen-deuterium exchange mass spectrometry to identify allosterically
relevant changes in proteins. Prassannan, C.B., Villar, M.T., Artigues, A., and
Fenton A.W. 10th International Symposium on Mass Spectrometry in the Health
and Life Sciences: Molecular and Cellular Proteomics. San Francisco, CA August
21-25 (2011).
The Structure of early folding intermediates of mitochondrila aspartate
aminotransferase and its reduced form. Villar, M.T. and Artigues, A. 10th
International Symposium on Mass Spectrometry in the Health and Life Sciences:
Molecular and Cellular Proteomics. San Francisco, CA August 21-25 (2011).
Identification of regions of rabbit muccle pyruvate kinase important for allosteric
regulation by phenylalanine detected by deuterium-hydrogen exchange mass
spectrometry. Prassannan, C.B., Villar, M.T., Artigues, A., and Fenton A.W. 23rd
ASMS Sanibel Conference. From fragmentation mechanisms to sequencing:
Tandem Mass Spectrometry Based Peptide and Protein Identification. Sanibel,
FL January 21-24 2012.
H. Changotra, A. Artigues and L. Hutt-Fletcher. The cellular protein p32 interacts
with the cytoplasmic tail of EBV gM and is required for efficient egress of virions
from the cell. 35th International Herpesvirus Workshop. Salt Lake City, Utah July
24-29 2010. .
L. Novikova, L. Stehno-Bittel, A. Artigues, M.T. Villar, S. J. Williams, H.-H.
Huang, K. Kover and I. V. Smirnova. Pancreatic islet transplantation to treat
diabetes – defining molecular tools to select suitable islets. Missouri Regional
Life Sciences Summit, "Animal to Human Health Collaborations – Regional
Partnerships for Innovation." Kansas City, MO March 8th, 2010.
M.T. Villar, D. E. Miller, A.W. Fenton and A. Artigues. Deuterium exchange mass
spectrometry using a semiautomatic interface for data acquisition and automatic
data analysis and reporting. ASMS 57th annual Conference on Mass
Spectrometry and Allied Topics annual Conference. Philadelphia, PA, May 31June 4, 2009.
7
M M. T. Villar and A. Artigues: Folding dependence of a Mitochondrial Precursor
Protein on elements of its primary sequence Protein Society meeting, San Diego,
2008.
D. E. Miller, A. Artigues and A. W. Fenton. H/DX-MS detection of changes in
protein dynamics of pyruvate kinase elicited by binding of allosteric vs.
nonallosteric effector analogs (oral presentation). 22nd Annual Gibbs Conference
on Biothermodynamics October, 2008
Y Dong, PhD1, W Hou, PhD1, A Artigues, PhD2, M Villar, PhD2 and C P Weiner,
MD, MBA1. Fetal adaptation to chronic hypoxemia (hpx) alters the Proteome of
the fetal brain in a dose dependent fashion. Sgi 2008 annual scientific meeting.
M.T. Villar and A. Artigues. Refolding of cytosolic aspartate aminotransferase
analyzed by hydrogen exchange and mass spectrometry. 21th Symposium of the
Portein Society, July 21-25, 2007
M. T. Villar, Z. V. Niatsetskaya, A. J. L. Cooper, and A. Artigues Inactivation of
rat liver mitochondrial aspartate aminotransferase by a halogenated cysteine
conjugate 8th on Mass Spectrometry in the Health and Life Sciences. San
Francisco, 2007
Owen Nadeau, Justin Paschall, Gerald Wyckoff, Antonio Artigues, Jessica Sage,
Maria Teresa Villar, Gerald Carlson Detection of Intrasubunit Interactions in the
Regulatory Beta Subunit in the exadecameric Phosphorylase Kinase Complex
Using a Data-based Search Engine to Detect Chemically Cross-linked Peptides
by Mass Spectrometry 21th Symposium of the Protein Society, July 21-25, 2007
O. W. Nadeau, A. Artigues, J.E. Paschall, G.J. Wyckoff and G.M. Carlson, “
Mapping a Phosphorylation-dependent Subunit Communication Network in the
Hexadecameric Phosphorylase kinase Complex Using a Data-based Search
engine to Detect Chemically Cross-linked Peptides by Mass Spectrometry.”
Protein Science 15, Abs. # 457, 224, 2006
M. Jeyasingham, A. Artigues, G. Carlson: A Phosphoproteomic Approach to
Identifying Kinases that use Phosphoenolpyruvate as a Phosphoryl Donor. 20th
Symposium of the Portein Society, August 5-9, 2006
A. Artigues, J. A. Oses-Prieto, A. Iriarte, and M. Martinez-Carrion: Probing the
molten globule structure of a globular protein on a Ion Clyclotron-Fourrier
Transform Mass Spectrometer in combination with Hydrogen Exchange. 6th
European Symposium of the Protein Society. Barcelona, Spain, April 30- May 4,
2005
Owen W. Nadeau, David Anderson, Qing Yang, Antonio Artigues and Gerald M.
Carlson: The N-terminus of the regulatory β subunit of phosphorylase kinase
mediates self-association of this subunit in the activated holoenzyme complex.
18th Symposium of the Protein Society, San Diego, CA: August 14-18, 2004.
J. A. Oses-Prieto, A. Artigues, A. Iriarte, M. Martinez-Carrion: Refolding of
Aspartate Aminotransferase Analyzed by Hydrogen Exchange and Mass
Spectrometry. 6th symposium on Mass Spectrometry in the Health and Life
Sciences. San Francisco, 2003
8
A. Artigues, A. Iriarte, and M. Martinez-Carrion: Binding to Chaperones and
Import of a Purified precursor Protein into Mitochondria. 16th symposium of the
Protein Society. San Diego, CA. August 17-21, 2002..
M. Jeyasingham, A. Artigues, N. Nadeau, and G. Carlson: Zero-Lengh
Crosslinking of the Intrinsic Catalytic Gamma Subunit of Phosphorylase Kinase
with Either its Regulatory Alpha or delta Subunits Is Oppositley Affected by Ca2+.
16th symposium of the Protein Society. August 17-21, 2002. San Diego, CA.
A. Artigues, A. Iriarte, and M. Martinez-Carrion:Import of a Mitochondrial
Precursor Complexed to Hsc70 Into Isolated mitochondria. Fifth International
Symposium on Mass Spectrometry in the Health and Life Sciences: Molecular
and Cellular Proteomics. August 26-30, 2001 San Francisco, CA.
J. Oses-Prieto, A. Artigues, A. Iriarte and M. Martinez-Carrion: The presence of
covalently attached cofactor can bias the interpretation of protein refolding
kinetics.
FASEB, Experimetnal Biology 2001 Meetting. Orlando, Forida. USA (2001).
C. Torella, J. R. Mattingly, Jr., A. Artigues, A. Iriarte, and M. Martinez-Carrion:
Insight into the conformation of folding intermediates of a protein trapped by
GroEL. Forty-second Annual Meeting of the Biophysical Society. Kansas City,
Missouri, USA (1998).
A. Artigues, A. Iriarte and M. Martinez-Carrion: Mapping the binding sites of
hsp70 into a large mitochondrial precursor protein. Protein Society. San Joss,
California, USA (1996).
A. Artigues, A. Iriarte and M. Martinez-Carrion: hsp70 can interact in vitro with an
unfolded mitochondrial precursor protein but not with its cytosolic isozyme.
Protein Society. Boston, MD, USA (1995).
A. Artigues, A. Iriarte and M. Martinez-Carrion: In vitro refolding of Mitochondrial
Aspartate Aminotransferase. ASBMB/ACS Joint meeting. San Diego, California,
USA. (1993).
A. Artigues, A. Fernandez and J. M. González-Ros: Purificación de subunidades
del Receptor Nicotínico de Acetilcolina mediante electroelución contínua.
Congreso de Neurociencias. Alicante. (1991).
A. Artigues and V. Schirch: Role of the aminoterminal region of serine
hydroxymethyltransferase in determining its in vivo rate of degradation. FASEB
Summer Research Conference on Folic acid, B12 and One Carbon Metabolism.
Xaxon, Vermont, USA (1990).
A. Artigues and V. Schirch: Role of the aminoterminal region of serine
hydroxymethyltransferase in determining its in vivo rate of degradation.
ASBMB/AAI Joint meeting, New Orleans, USA (1990).
J. M. González-Ros, M. T. Villar, A. Artigues y J.A. Ferragut: Dependencia de
parametros estructurales del receptor nicotínico de acetilcolina con su entorno
9
lipídico. II Congreso de la Sociedad Española de Neurociencias. Barcelona,
España (1987).
M. T. Villar, A. Artigues, J. M. González-Ros: Modificación de las propiedades
estructurales del receptor nicotínico de acetilcolina por la Fosfolipasa A2. XIII
Congreso Nacional de la Sociedad Espalñola de Bioquímica. Zaragoza, España
(1986).
J. M. González-Ros, A. Artigues, M. T. Villar y J. A. Ferragut: Heat inactivation
studies of membrane-bound AcChR from Torpedo: Effect of cholinergic ligands
and membrane perturbants. 3td Symposium Handling of environmental and
biological samples in chromatography. Palma de Mallorca, España (1986).
M. T. Villar, A. Artigues, J. Ferragut and J. M. González-Ros: Modificación de las
propiedades del receptor nicotínico de acetilcolina por proteínas periféricas de
membrana. XII Congreso Nacional de la Sociedad Española de Bioquímica.
Valencia, España (1985)
Ongoing Research Support
R01DE018713 (J. Wang, PI)
Bone sialoprotein in osteogenesis and bone regeneration
Goal: to study the mechanism of bone sialprotein-mediated osteogenesis and
bone regeneration
Role: Collaborator
2R56DK032953-26 (G.M. Carlson, PI)
Subunit Interactions of Phosphorylase Kinase
Goal: To study the structure, mechanism of action and regulation of
phosphorylase kinase
Role: Collaborator
1 R01 DK078076-01A1 (A.W. Fenton, PI)
Dissecting Allostery in Pyruvate Kinase
Goal: To study the allsoteric regulation and dynamics of Pyruvate kinase
Role: Collaborator
Completed Research Support
NIH - R21 DK072393-02 (G.M. Carlson, PI)
9/01/05 - 6/30/08
Title: Proteomics to screen for a protein kinase that uses PEP
Role: Collaborator.
10
NIH - 2 R56 HL067336-05A (K.R. Peterson, PI)
7/15/08 - 7/14/09
Title: Locus linked regulatory motifs of globin gene switching
Role: Other Significant Contributor
NIH - 5 P20 RR024214-02 (D.R. Abrahamson, PI; L. Christenson, subproject
leader)
9/27/07 - 6/30/12
Title: Molecular Regulation of Cell Development and Differentiation
Role: Collaborator
11
CURRICULUM VITAE
James P. Calvet
Address and Phone
Department of Biochemistry and Molecular Biology
6001 Wahl Hall East, Kidney Institute
University of Kansas Medical Center, MS3018
Kansas City, Kansas 66160
Tel: (913) 588-7424 Fax: (913) 588-9251
Email: [email protected]
Date and Place of Birth: November 6, 1945; Washington, D.C.
Family Status: Married; three daughters
Education
University of Connecticut
Ph.D.
Storrs, Connecticut
State University College
1970
Genetics and
1975
Cell Biology
M.A.
Biology
B.A.
Biology
Plattsburgh, New York
Franklin Pierce College
1968
Rindge, New Hampshire
Professional Experience
2012-present
Deputy Director, Kidney Institute, University of Kansas Medical
Center
2009-present
Co-Leader, Cancer Biology Program, University of Kansas Cancer
Center
12
2009-2011
Interim Director, Kidney Institute, University of Kansas Medical
Center
2005-present
Director, Kansas Interdisciplinary Center for PKD Research,
University of Kansas Medical Center
1994-present
Professor (tenured), Department of Biochemistry and Molecular
Biology, School of Medicine, University of Kansas Medical Center
1988-1994
Associate Professor (tenured), Department of Biochemistry and
Molecular Biology, School of Medicine, University of Kansas
Medical Center
1981-1988
Assistant Professor, Department of Biochemistry, School of
Medicine, University of Kansas Medical Center
1978-1981
Senior Research Associate (with Dr. Thoru Pederson),
Department of Cell Biology, Worcester Foundation for
Experimental Biology
1975-1978
Postdoctoral Fellow in Cell Biology (with Dr. Thoru Pederson),
American Cancer Society Postdoctoral Fellow (1976-1977),
Worcester Foundation for Experimental Biology
Honors and Awards
Student Voice Award for Excellence in Teaching Medical Biochemistry, 1996-97.
Investigator Research Award, School of Medicine, KUMC 2004.
Chancellor’s Distinguished Teaching Award for Excellence in Teaching, University of
Kansas, 2007
Chancellor’s Club Research Award, University of Kansas, 2009
Lillian Jean Kaplan International Prize for Advancement in the Understanding of
Polycystic Kidney Disease, awarded jointly by the International Society of
Nephrology and PKD Foundation, 2011.
John C. Davis Memorial Lecture, University of Kansas, 2012
Professional Societies
American Society of Biochemistry and Molecular Biology
American Society for Cell Biology
American Society of Nephrology
International Society of Nephrology
American Association for the Advancement of Science
Research Interests
13
Molecular biology and molecular genetics of polycystic kidney disease; regulation of cell
proliferation, signal transduction, transcriptional mechanisms, gene expression in normal
and abnormal kidney development; cellular, molecular, and developmental biology;
human genetics and cancer.
Publications (peer reviewed)
Laufer, H. and Calvet, J.P. (1972) Hormonal effects on chromosomal puffs and insect
development. Gen. and Comp. Endocr. Suppl. 3, 137-148.
Calvet, J.P. and Clark, R.M. (1974) Immersion refractometric analysis of cultured Hyalophora
cecropia hemocytes. Protoplasma 80, 29-40.
Calvet, J.P. and Pederson, T. (1977) Secondary structure of heterogeneous nuclear RNA: Two
classes of double-stranded RNA in native ribonucleoprotein. Proc. Natl. Acad. Sci. U.S.A.
74, 3705-3709.
Calvet, J.P. and Pederson, T. (1978) Nucleoprotein organization of inverted repeat DNA
transcripts in heterogeneous nuclear RNA-ribonucleoprotein particles from HeLa cells. J.
Mol. Biol. 122, 361-378.
Calvet, J.P. and Pederson, T. (1979) Heterogeneous nuclear RNA double-stranded regions
probed in living HeLa cells by cross-linking with the psoralen derivative
aminomethyltrioxsalen. Proc. Natl. Acad. Sci. U.S.A. 76, 755-759.
Calvet, J.P. and Pederson, T. (1979) Photochemical cross-linking of secondary structure in HeLa
cell heterogeneous nuclear RNA in situ. Nuc. Acids Res. 6, 1993-2001.
Calvet, J.P. and Pederson, T. (1981) Base-pairing interactions between small nuclear RNAs
and nuclear RNA precursors as revealed by psoralen cross-linking in vivo. Cell 26,
363-370.
Calvet, J.P., Meyer, L.M. and Pederson, T. (1982) Small nuclear RNA U2 is base-paired to
heterogeneous nuclear RNA. Science 217, 456-458.
Roberts, M.P., Falvey, E., Calvet, J.P. and Pederson, T. (1983) A sequence related to 4.5S
RNA and the B1 family of repeated DNA in the 5' flanking region of the mouse β-globin
gene. J. Mol. Biol. 169, 975-982.
Kunkel, G., Maser, R.L., Calvet, J.P. and Pederson, T. (1986) U6 small nuclear RNA is
transcribed by RNA polymerase III. Proc. Natl. Acad. Sci. U.S.A. 83, 8575-8579.
14
Smardo, F.L., Jr. and Calvet, J.P. (1987) Human glutamate tRNA forms stable hybrids in vitro
with 28S ribosomal RNA. Nuc. Acids Res. 15, 661-681.
Smardo, F.L., Jr. and Calvet, J.P. (1987) Sequence analysis of the glutamate tRNA family:
evidence for pseudogenes. Gene 57, 213-220.
Andrews, G.K., Harding, M.A., Calvet, J.P. and Adamson, E.D. (1987) The heat shock response
in HeLa cells is accompanied by elevated expression of the c-fos proto-oncogene. Mol.
Cell. Biol. 7, 3452-3458.
Calvet, J.P. and Myers, J.A. (1987) In vivo secondary structure analysis of the small nuclear
RNA U1 using psoralen cross-linking. J. Mol. Biol. 197, 543-553.
Cowley, B.D., Jr., Smardo, F.L., Jr., Grantham, J.J. and Calvet, J.P. (1987) Elevated c-myc
proto-oncogene expression in autosomal recessive polycystic kidney disease. Proc. Natl.
Acad. Sci. U.S.A. 84, 8394-8398.
Gattone, V.H., Calvet, J.P., Cowley, B.D., Jr., Evan, A.P., Shaver, T.S., Helmstadter, K. and
Grantham, J.J. (1988) Autosomal recessive polycystic kidney disease in a murine model: a
gross and microscopic description. Lab. Invest. 59, 231-238.
Cowley, B.D., Jr., Chadwick, L.J., Grantham, J.J. and Calvet, J.P. (1989) Sequential protooncogene expression in regenerating kidney following acute renal injury. J. Biol. Chem.
264, 8389-8393.
Maser, R.L. and Calvet, J.P. (1989) U3 small nuclear RNA can be psoralen cross-linked in vivo
to the 5' external transcribed spacer of pre-ribosomal RNA. Proc. Natl. Acad. Sci. U.S.A.
86, 6523-6527.
Mangoo-Karim, R., Uchic, M.E., Grant, M., Shumate, W.A., Calvet, J.P., Park, C.H. and
Grantham, J.J. (1989) Renal epithelial fluid secretion and cyst growth: The role of cyclic
AMP. FASEB J. 3, 2629-2632.
Grantham, J.J., Mangoo-Karim, R., Uchic, M.E., Grant, M., Shumate, W.A., Park, C.H. and Calvet, J.P.
(1989) Net fluid secretion by mammalian renal epithelial cells: stimulation by cAMP in polarized
cultures derived from established renal cells and from normal and polycystic kidneys. Trans.
Assoc. Am. Physicians 102, 158-162.
Gattone, V.H., Andrews, G.K., Niu Fu-wen, Chadwick, L.J., Klein, R.M. and Calvet, J.P. (1990) Defective
epidermal growth factor gene expression in mice with polycystic kidney disease. Dev. Biol. 138,
225-230.
15
Takahashi, H., Calvet, J.P., Dittemore-Hoover, D., Yoshida, K., Grantham, J.J. and Gattone, V.H. (1991) A
hereditary model of slowly progressive polycystic kidney disease in the mouse. J. Am. Soc.
Nephrol. 1, 980-989.
Cowley, Jr., B.D., Chadwick, L.J., Grantham, J.J. and Calvet, J.P. (1991) Elevated proto-oncogene
expression in polycystic kidneys of the C57BL/6J (cpk) mouse. J. Am. Soc. Nephrol. 1, 1048-1053.
Gattone, V.H. and Calvet, J.P. (1991) Murine infantile polycystic kidney disease: a role for reduced renal
epidermal growth factor. Am. J. Kid. Dis. 17, 606-607.
Harding, M.A., Chadwick, L.J., Gattone, V.H. and Calvet, J.P. (1991) The SGP-2 gene is developmentally
regulated in the mouse kidney and abnormally expressed in collecting duct cysts in polycystic
kidney disease. Dev. Biol. 146, 483-490.
Harding, M.A., Gattone, V.H., Grantham, J.J. and Calvet, J.P. (1992) Localization of overexpressed c-myc
mRNA in polycystic kidneys of the cpk mouse. Kidney Int. 41, 317-325.
Rankin, C.A., Grantham, J.J. and Calvet, J.P. (1992) c-fos expression is hypersensitive to serumstimulation in cultured cystic kidney cells from the C57BL/6J-cpk mouse. J. Cell. Physiol. 152, 578586.
Birkenmeier, E.H., Letts, V.A., Frankel, W.N., Magenheimer, B.S. and Calvet, J.P. (1993) Sulfated
glycoprotein-2 (Sgp-2) maps to mouse chromosome 14. Mammalian Gen. 4, 131-132.
Cowley, Jr., B.D., Gudapaty, S., Kraybill, A.L., Harding, M.A., Calvet, J.P. and Gattone, V.H. (1993)
Autosomal dominant polycystic kidney disease in the rat. Kidney Int. 43, 522-534.
Lowden, D.A., Lindemann, G.W., Merlino, G., Barash, B.D., Calvet, J.P. and Gattone, V.H. (1994) Renal
cysts in transgenic mice expressing transforming growth factor-α. J. Lab. Clin. Med. 124, 386-394.
Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1994) Mouse plasma glutathione peroxidase: cDNA
sequence analysis and renal proximal tubule expression and secretion. J. Biol. Chem. 269, 2706627073.
Calvet, J.P. and Chadwick, L.J. (1994) Primary and secondary genetic responses following folic acidinduced acute renal injury in the mouse. J. Am. Soc. Nephrol. 5, 1324-1332.
Maser, R.L. and Calvet, J.P. (1995) Analysis of differential gene expression in the kidney by differential
cDNA screening, subtractive cloning, and mRNA differential display. Sem. Nephrol. 15, 29-42.
Rankin, C.A., Ziemer, D.M., Maser, R.L., Foo, I. and Calvet, J.P. (1996) Growth characteristics of cells
cultured from two murine models of polycystic kidney disease. In Vitro Cell. Dev. Biol. 32, 100106.
Hou, X., Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1996) A mouse kidney- and liver-expressed
cDNA having homology with a prokaryotic parathion hydrolase (phosphotriesterase) gene:
abnormal expression in injured and polycystic kidneys. Gene 168, 157-163.
Gattone, V.H., Kuenstler, K.A., Lindemann, G.W., Lu, X., Cowley, B.D., Rankin, C.A. and Calvet, J.P.
(1996) Renal expression of a transforming growth factor alpha transgene accelerates the
progression of inherited, slowly progressive polycystic kidney disease in the mouse. J. Lab. Clin.
Med. 127, 214-222.
Davidow, C.J., Maser, R.M., Rome, L.A., Calvet, J.P. and Grantham, J.J. (1996) The cystic fibrosis
transmembrane conductance regulator (CFTR) mediates transepithelial fluid secretion by human
autosomal dominant polycystic kidney disease (ADPKD) epithelium in vitro. Kidney Int. 50, 208218.
Rankin, C.A., Suzuki, K., Itoh, Y., Ziemer, D.M., Grantham, J.J., Calvet, J.P. and Nagase, H. (1996) Matrix
metalloproteinases and TIMPS in cultured C57BL/6J-cpk kidney tubules. Kidney Int. 50, 835-844.
Parnell, S.C., Magenheimer, B.S., Maser, R.L., Rankin, C.A., Smine, A., Okamoto, T. and Calvet, J.P.
(1998) The polycystic kidney disease-1 protein, polycystin-1, binds and activates heterotrimeric Gproteins in vitro. Biochem. Biophys. Res. Comm. 251, 625-631.
Witzgall, R., Obermuller, N., Bolitz, U., Calvet, J.P., Cowley, B.D., Walker, C., Kriz, W., Gretz, N. and
Bonventre, J.V. (1998) Kid-1 expression is high in differentiated renal proximal tubule cells and
suppressed in cyst epithelia. Am. J. Physiol. 275, F928-F937.
Rankin, C.A., Itoh, Y., Tian, C., Ziemer, D.M., Calvet, J.P. and Gattone, V.H. (1999) Matrix
metalloproteinase 2 in a murine model of infantile polycystic kidney disease. J. Am. Soc. Nephrol.
10, 210-217.
Parnell, S.C., Magenheimer, B.S., Maser, R.L. and Calvet, J.P. (1999) Identification of the major site of in
vitro PKA phosphorylation in the polycystin-1 C-terminal cytosolic domain. Biochem. Biophys.
Res. Comm. 259, 539-543.
Ricker, J.L., Gattone, V.H., Calvet, J.P. and Rankin, C.A. (2000) Development of autosomal recessive
polycystic kidney disease in Balb-c cpk/cpk mice. J. Am. Soc. Nephrol. 11, 1837-1847.
Sutters, M., Yamaguchi, T., Maser, R.L., Magenheimer, B.S., St. John, P.L., Abrahamson, D.R., Grantham,
J.J. and Calvet, J.P. (2001) Polycystin-1 transforms the cAMP growth-responsive phenotype of M-1
cells. Kidney Int. 60, 484-494.
Maser, R.L., Vassmer, D., Magenheimer, B.S. and Calvet, J.P. (2002) Oxidant stress and reduced
antioxidant enzyme protection in polycystic kidney disease. J. Am. Soc. Nephrol. 13, 991-999.
Ledford, A.W., Brantley, J.G., Kemeny, G., Foreman, T.L., Quaggin, S.E., Igarashi, P., Oberhaus, S.M.,
Rodova, M., Calvet, J.P. and Vanden Heuvel, G.B. (2002) Deregulated expression of the homeobox
gene Cux-1 in transgenic mice results in down regulation of p27kip1 expression during
nephrogenesis, glomerular abnormalities, and multiorgan hyperplasia. Dev. Biol. 245, 157-171.
Parnell, S.C., Magenheimer, B.S., Maser, R.L., Zien, C.A., Frischauf, A.-M. and Calvet, J.P. (2002)
Polycystin-1 mediated activation of c-Jun-N-terminal kinase and AP-1 is regulated by heterotrimeric
G proteins. J. Biol. Chem. 277, 19566-19572.
Wolf, D., Rodova, M., Miska, E.A., Calvet, J.P. and Kouzarides, T. (2002) Acetylation of -catenin by
CREB-binding protein (CBP). J. Biol. Chem. 277, 25562-25567.
Rodova, M., Islam, M.R., Maser, R.L. and Calvet, J.P. (2002) The polycystic kidney disease-1 promoter is
a target of the 
-catenin/T-cell factor pathway. J. Biol. Chem. 277, 29577-29583.
Islam, M.R., Rodova, M. and Calvet, J.P. (2002) A fast and efficient method of DNA fragment isolation
from agarose gels without using commercial kits. Amer. Biotech. Lab. 20, 18.
Rodova, M., Islam, M.R., Peterson, K.R. and Calvet, J.P. (2003) Remarkable sequence conservation of the
last intron in the PKD1 gene. Molec. Biol. Evol. 20, 1669-1674.
Le, N.H., Van Der Bent, P., Huls, G., Van De Wetering, M., Loghman-Adham, M., Ong, A.C., Calvet, J.P.,
Clevers, H., Breuning, M.H., Van Dam, H. and Peters, D.J. (2004) Aberrant polycystin-1 expression
results in modification of AP-1 activity, while Wnt signaling remains unaffected. J. Biol. Chem. 279,
27472-27481.
Yamaguchi, T., Wallace, D.P., Magenheimer, B.S., Hempson, S.J., Grantham, J.J., and Calvet, J.P. (2004)
Calcium restriction allows cAMP activation of the B-Raf/ERK pathway, switching cells to a cAMPdependent growth-stimulated phenotype. J. Biol. Chem. 279, 40419-40430.
Puri, S., Magenheimer, B.S., Maser, R.L., Ryan, E., Zien, C.A., Walker, D.D., Wallace, D.P., Hempson,
S.J., and Calvet, J.P. (2004) Polycystin-1 activates the calcineurin/NFAT (nuclear factor of activated
T-cells) signaling pathway. J. Biol. Chem. 279, 55455-55464.
Puri, S., Rodova, M., Islam, M.R., Magenheimer, B.S., Maser, R.L., and Calvet, J.P. (2006) Ets factors
regulate the polycystic kidney disease-1 (PKD1) promoter. Biochem. Biophys. Res. Comm. 342,
1005-1013.
Xiao, Z., Zhang, S., Malios, J., Zhou, G., Magenheimer, B.S., Guo, D., Dallas, S.L., Maser, R., Calvet, J.P.,
Bonewald, L., Quarles, L.D. (2006) Cilia-like structures and polycystin-1 in osteoblasts/osteocytes
and associated abnormalities in skeletogenesis and Runx2 expression. J. Biol. Chem. 281,
30884-30895.
Van Bodegom, D., Saifudeen, Z., Dipp, S., Puri, S., Magenheimer, B.S., Calvet, J.P., and El-Dahr, S.S.
(2006) The polycystic kidney disease-1 gene is a target for p53-mediated transcriptional repression.
J. Biol. Chem. 281, 31234-31244.
Magenheimer, B.S., St. John, P.L., Isom, K.S., Abrahamson, D.R., De Lisle, R.C., Wallace, D.P., Maser,
R.L., Grantham, J.J., and Calvet, J.P. (2006) Early embryonic renal tubules of wild-type and PKD
kidneys respond to cAMP stimulation with CFTR/NKCC1-dependent cystic dilation. J. Am. Soc.
Nephrol. 17, 3424-3437.
Nagao, S., Nishii, K., Yoshihara, D., Kurahashi, H., Nagaoka, K., Yamashita, T., Takahashi, H.,
Yamaguchi, T., Calvet, J.P., and Wallace, D.P. (2008) Calcium channel inhibition with verapamil
accelerates polycystic kidney disease progression in the Cy/+ rat. Kidney Int. 73, 269-277.
Li, X., Magenheimer, B.S., Xia, S., Johnson, T., Wallace, D.P., Calvet, J.P., and Li, R. (2008) A tumor
necrosis factor-alpha-mediated pathway promoting autosomal dominant polycystic kidney disease.
Nature Medicine 14, 863-868.
Islam, M.R., Puri, S., Rodova, M., Magenheimer, B.S., Maser, R.L., and Calvet, J.P. (2008) Retinoic aciddependent activation of the polycystic kidney disease-1 (PKD1) promoter. Am. J. Physiol. Renal
Physiol. 295, F1845-1854.
Katayama, H., McGill, M., Kearns, A., Brzozowski, M., Degner, N., Harnett, B., Kornilayev, B., MatkovićČalogović, D., Holyoak, T., Calvet, J.P., Gogol, E.P., Seed, J., Fisher, M.T. (2009) Strategies for
folding of affinity tagged proteins using GroEL and osmolytes. J. Struct. Funct. Genomics. 10(1),
57-66.
Nagao, S., Morita, M., Kugita, M., Yoshihara, D., Yamaguchi, T., Kurahashi, H., Calvet, J.P., and Wallace,
D.P. (2010) Polycystic kidney disease in Han:SPRD Cy rats is associated with elevated expression
and mislocalization of SamCystin. Am. J. Physiol. Renal Physiol. 299(5), F1078-1086.
Yamaguchi, T., Reif, G.A., Calvet, J.P., and Wallace, D.P. (2010) Sorafenib inhibits cAMP-dependent ERK
activation, cell proliferation, and in vitro cyst growth of human ADPKD cyst epithelial cells. Am. J.
Physiol. Renal Physiol. 299(5), F944-951.
Islam, M.R., Jimenez, T., Pelham, C., Rodova, M., Puri, S., Magenheimer, B.S., Maser, R.L., Widmann, C.,
and Calvet, J.P. (2010) MAP/ERK kinase kinase 1 (MEKK1) mediates transcriptional repression by
interacting with polycystic kidney disease-1 (PKD1) promoter-bound p53 tumor suppressor protein.
J. Biol. Chem. 285, 38818-38831.
Kugita, M., Nishii, K., Morita, M., Yoshihara, D., Kowa-Sugiyama, H., Yamada, K., Yamaguchi, T., Wallace,
D.P., Calvet, J.P., Kurahashi, H., and Nagao, S. (2011) Global gene expression profiling in earlystage polycystic kidney disease in the Han:SPRD Cy rat identifies a role for RXR signaling. Am. J.
Physiol. Renal Physiol. 300 (1), F177-F188.
Yoshihara, D., Kurahashi, H., Morita, M., Kugita, M., Hiki, Y., Aukema, H.M., Yamaguchi, T., Calvet, J.P.,
Wallace, D.P., and Nagao, S. (2011) PPAR-γ agonist ameliorates kidney and liver disease in an
orthologous rat model of human autosomal recessive polycystic kidney disease. Am. J. Physiol.
Renal Physiol. 300 (2), F465-474.
Fan, L.X., Li, X., Magenheimer, B.S., Calvet, J.P., Li, X. (2012) Inhibition of histone deacetylases targets
the transcription regulator Id2 to attenuate cystic epithelial cell proliferation. Kidney Int. 81 (1), 7685.
Parnell, S.C., Puri, S., Wallace, D.P., and Calvet, J.P. (2012) Protein phosphatase-1
dephosphorylates polycystin-1. PLoS ONE, 7 (6) e36798 (11 pages).
 inte ra cts with
and
Yoshihara, D., Kugita, M., Yamaguchi,T., Aukema, H.M., Kurahashi, H., Morita, M., Hiki, Y., Calvet, J.P.,
Wallace, D.P., Toyohara, T., Abe, T., Nagao, S. (2012) Global Gene Expression Profiling in PPAR-γ
Agonist-Treated Kidneys in an Orthologous Rat Model of Human Autosomal Recessive Polycystic
Kidney Disease. PPAR Res. 2012, 695898 (10 pages).
Jansson, K., Nguyen, A., Magenheimer, B.S., Reif, G.A., Reddy, A.L., Bello-Reuss, E., Wallace, D.P.,
Calvet, J.P., and Blanco, G. (2012) Endogenous concentrations of ouabain act as a cofactor to
stimulate fluid secretion and cyst growth of in vitro ADPKD models via cAMP and EGFR-Src-MEK
pathways. Am. J. Physiol. Renal Physiol. 303 (7), F982-990.
Chapters, Reviews (invited)
Calvet, J.P. (1990) Molecular Biology, Gene Expression, and Medicine. In Topics in Renal Medicine:
Inheritance of Kidney and Urinary Tract Diseases (Spitzer, A. and Avner, E.D., eds.) Kluwer,
Boston, pp. 3-51. (book chapter).
Calvet, J.P. and Cowley, B.D., Jr. (1990) Proto-Oncogene Expression in Autosomal Recessive Polycystic
Kidney Disease. In Advances in the Pathogenesis of Polycystic Kidney Disease (Carone, F.A. and
Dobbie, J.W., eds.) TP&T, Chicago, pp. 33-36. (review).
Calvet, J.P. (1991) Molecular approaches for analyzing differential gene expression: differential cDNA
library construction and screening. Pediatr. Nephrol. 5, 751-757. (basic science review).
Calvet, J.P. (1993) Polycystic kidney disease: primary extracellular matrix abnormality or defective cellular
differentiation? Kidney Int. 43, 101-108. (review and opinion).
Calvet, J.P. (1993) Proto-Oncogenes and Abnormalities of Differentiation in PKD. In Proceedings of the
Fifth International Workshop on Polycystic Kidney Disease (Gabow, P.A. and Grantham, J.J., eds.)
Polycystic Kidney Research Foundation, Kansas City, pp. 108-113. (review and update).
Calvet, J.P. (1994) Injury and development in polycystic kidney disease. Curr. Opin. Nephrol. Hypertens.
3, 340-348. (review and opinion).
Calvet, J.P. (1995) Abnormal epithelial cell proliferation in renal cyst formation and growth: The maturation
arrest hypothesis. Kidney Int. 47, 715-716. (review of meeting talk).
Calvet, J.P. (1996) Principles of Molecular Biology as Applied to the Study of Disease. In Polycystic Kidney
Disease (Watson, M.L. and Torres, V.E., eds.) Oxford University Press, Oxford, pp. 3-38. (book
chapter).
Calvet, J.P. (1998) Molecular genetics of polycystic kidney disease. J. Nephrol. 11, 24-34. (basic science
review).
Calvet, J.P. (1998) Comprehensive sequence analysis. Science 282, 1057-1058. (software review).
Grantham, J.J. and Calvet, J.P. (2001) Polycystic kidney disease: In danger of being X-rated? Proc. Natl.
Acad. Sci. USA 98, 790-792. (editorial commentary).
Calvet, J.P. and Grantham, J.J. (2001) The genetics and physiology of polycystic kidney disease. Sem.
Nephrol. 21, 107-123. (comprehensive review).
Calvet, J.P. (2002) Cilia in PKD — Letting it all hang out. J. Am. Soc. Nephrol. 13, 2614-2616. (invited
editorial review).
Maser, R.L., Magenheimer, B.S., Zien, C.A. and Calvet, J.P. (2003) Transient Transfection Assays for
Analysis of Signal Transduction in Renal Cells. In Methods in Molecular Medicine, Renal Disease
Techniques and Protocols. M.S. Goligorsky, ed. Humana Press, Totowa, N.J., pp. 205-217.
(methods chapter).
Calvet, J.P. (2003) Ciliary signaling goes down the tubes. Nature Genetics 33, 113-114. (news & views).
Calvet, J.P. (2003) New insights into ciliary function: kidney cysts and photoreceptors. Proc. Natl. Acad.
Sci. USA 100, 5583-5585. (invited commentary).
Calvet, J.P. (2006) MEK inhibition holds promise for polycystic kidney disease. J. Am. Soc. Nephrol.
17(6):1498-1500. (editorial).
Calvet, J.P. (2008) Strategies to inhibit cyst formation in ADPKD. Clin. J. Am. Soc. Nephrol. 3, 1205-1211
(summary of invited talk).
Abstracts
Calvet, J.P., Laufer, H., and Krider, H. (1976) Repetitive nucleotide sequences in the Chironomus genome.
J. Cell Biol. 70, 291a (Abstract).
Calvet, J.P. and Pederson, T. (1976) Characterization of double-stranded sequences in HeLa cell
heterogeneous nuclear RNA-ribonucleoprotein particles. J. Cell Biol. 70, 291a (Abstract).
Calvet, J.P. and Pederson, T. (1977) Heterogeneous nuclear RNA packaging proteins: Role in modulating
the formation of hnRNA secondary structure. J. Cell Biol. 75, 414a (Abstract).
Calvet, J.P. and Pederson, T. (1977) Messenger RNA processing: Ribonucleoprotein organization of
double-stranded RNA sequences in HeLa cell heterogeneous nuclear RNA. J. Cell Biol. 75, 414a
(Abstract).
Calvet, J.P. and Pederson, T. (1978) Psoralens can be used to probe hnRNA secondary structure in living
HeLa cells. J. Cell Biol. 79, 345a (Abstract).
Calvet, J.P. and Pederson, T. (1981) Interactions between small nuclear RNAs and high molecular weight
precursors of mRNA and rRNA shown by cross-linking with psoralen in vivo. J. Cell Biol. 91, 137a
(Abstract).
Calvet, J.P. and Myers, J.A. (1982) Small nuclear RNA U4 is a major psoralen cross-linked RNA species in
HeLa cell nuclei. J. Cell Biol. 95, 479a (Abstract).
Calvet, J.P. and Myers, J.A. (1983) In vivo structure analysis of the small nuclear RNA U1 using psoralen
cross-linking. Fed. Proc. 42, 1882 (Abstract).
Calvet, J.P. and Myers, J.A. (1983) In vivo secondary structure and intermolecular base-pairing interactions
involving the small nuclear RNAs analyzed by psoralen cross-linking. RNA Processing, Cold Spring
Harbor Laboratory, p. 21 (Abstract).
Smardo, F.L., Jr. and Calvet, J.P. (1983) Identification of a small RNA that hybridizes specifically with high
molecular weight cytoplasmic RNA of HeLa cells. J. Cell Biol. 97, 358a (Abstract).
Smardo, F.L., Jr. and Calvet, J.P. (1984) Characterization of a 4S cytoplasmic RNA that hybridizes to 28S
ribosomal RNA of HeLa cells. Fed. Proc. 43, 2514 (Abstract).
Calvet, J.P., Smardo, F.L., Jr. and Maser, R.L. (1984) Small RNAs that do and do not interact with
ribosomal RNA. RNA Processing, Cold Spring Harbor Laboratory, p.29 (Abstract).
Maser, R.L. and Calvet, J.P. (1984) Nucleolar-specific U3 RNA interacts with nonribosomal nuclear RNA in
vivo. J. Cell Biol. 99, 144a (Abstract).
Smardo, F.L., Jr. and Calvet, J.P. (1984) Base-pairing interactions between small nuclear RNAs and
adenovirus 2 mRNA precursors. J. Cell Biol. 99, 144a (Abstract).
Maser, R.L. and Calvet, J.P. (1986) Nucleolar-specific U3 snRNA psoralen crosslinks to 28S-sized
nonribosomal RNA. RNA Processing, Cold Spring Harbor Laboratory, p. 114 (Abstract).
Kunkel, G.R., Maser, R.L., Calvet, J.P. and Pederson, T. (1986) U6 small nuclear RNA is transcribed by
RNA polymerase III. RNA Processing, Cold Spring Harbor Laboratory, p. 108 (Abstract).
Maser, R.L. and Calvet, J.P. (1987) U3 snRNA psoralen cross-links to RNA hybrid-selected by ETS/18S
ribosomal RNA sequences. RNA Processing, Cold Spring Harbor Laboratory, p. 119 (Abstract).
Cowley, B.D., Jr., Smardo, F.L., Jr., Grantham, J.J. and Calvet, J.P. (1987) Elevated c-myc expression in
mouse polycystic kidney disease. Kid. Int. 31, 163 (Abstract).
Maser, R.L. and Calvet, J.P. (1987) U3 snRNA psoralen cross-links to RNA hybrid-selected by ETS/18S
ribosomal RNA sequences. J. Cell Biol. 105, 68a (Abstract).
Harding, M.A., Calvet, J.P., Adamson, E.D. and Andrews, G.K. (1987) The heat shock response in HeLa
cells is accompanied by elevated expression of the c-fos proto-oncogene. J. Cell Biol. 105, 106a
(Abstract).
Cowley, B.D., Jr., Grantham, J.J. and Calvet, J.P. (1988) Elevated proto-oncogene expression following
acute renal injury. Kid. Int. 33, 152 (Abstract).
Cowley, B.D., Jr., Grantham, J.J. and Calvet, J.P. (1988) Elevated proto-oncogene expression in polycystic
kidney disease. FASEB J. 2, A839 (Abstract).
Harding, M.A. and Calvet, J.P. (1988) Translational effects on the stability of c-fos and c-myc mRNAs. J.
Cell Biol. 107, 100a (Abstract).
Maser, R.L. and Calvet, J.P. (1988) U3 snRNA interacts with the external transcribed spacer of the human
ribosomal RNA precursor near the +414 early processing site. J. Cell Biol. 107, 320a (Abstract).
Gattone, V.H., Fu-wen, N., Andrews, G.K., Klein, R.M. and Calvet, J.P. (1989) Reduced ontogenic
expression of epidermal growth factor (EGF) in a murine model of renal cystic disease. Kid. Int. 35,
311 (Abstract).
Grantham, J., Uchic, M., Mangoo-Karim, R., Shumate, W. and Calvet, J. (1989) Net fluid secretion by
mammalian renal epithelial cells: Stimulation by cAMP in polarized cultures derived from
established renal cell lines and from normal and polycystic kidneys. Clin. Res. 37, 612a (Abstract).
Calvet, J.P., Harding, M.A., Gattone, V.H. and Grantham, J.J. (1990) Localization of overexpressed c-myc
mRNA in the C57Bl/6J cpk murine model of polycystic kidney disease. J. Am. Soc. Nephrol. 1, 623
(Abstract).
Harding, M.A., Chadwick, L.J., Gattone, V.H. and Calvet, J.P. (1990) The SGP-2 gene is developmentally
regulated in the mouse kidney and is persistently expressed in collecting duct cysts in polycystic
kidney disease. J. Am. Soc. Nephrol. 1, 631 (Abstract).
Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1991) Identification of differentially expressed mRNAs in
polycystic kidneys of the cpk mouse. J. Am. Soc. Nephrol. 2, 257 (Abstract).
Rankin, C.A., Grantham, J.J. and Calvet, J.P. (1991) c-fos expression is hypersensitive to serumstimulation in cultured cystic kidney cells from the C57BL/6J-cpk mouse. J. Am. Soc. Nephrol. 2,
259 (Abstract).
Lowden, D.A., Merlino, G.T., Calvet, J.P., Barash, B.D. and Gattone, V.H. (1991) Renal pathology in
transforming growth factor-alpha (TGFα) transgenic mice. J. Am. Soc. Nephrol. 2, 440 (Abstract).
Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1992) Plasma glutathione peroxidase is a major kidney
mRNA and is differentially expressed in normal and polycystic cpk kidneys. J. Am. Soc. Nephrol. 3,
299 (Abstract).
Lindemann, G.W., Foo-Hurwitz, I., Magenheimer, B.S., Rankin, C.A. and Calvet, J.P. (1992) Isolation of
differentially expressed cDNAs in polycystic kidneys of the cpk mouse by subtractive cloning. J. Am.
Soc. Nephrol. 3, 298 (Abstract).
Maser, R.L., Magenheimer, B.S., Lindemann, G.W., Hou, X., Rankin, C.A. and Calvet, J.P. (1993)
Identification of differentially expressed cDNAs in the cystic kidneys of the C57BL/6J-cpk mouse. J.
Am. Soc. Nephrol. 4, 817 (Abstract).
Magenheimer, B.S. and Calvet, J.P. (1993) Altered expression of the mRNAs for HNF-1α, HNF-1β, and
HNF-4 transcription factors in the cystic kidneys of the C57BL/6J-cpk mouse. J. Am. Soc. Nephrol.
4, 817 (Abstract).
Foo, I., Davidow, C.J., Grantham, J.J. and Calvet, J.P. (1993) Effects of fibroblast-conditioned media on
morphological change and expression of the SGP-2 gene in MDCK cells. J. Am. Soc. Nephrol. 4,
813 (Abstract).
Maser, R.L. and Calvet, J.P. (1993) Androgen-responsive gene expression in kidney and submandibular
gland of cystic cpk mice. J. Am. Soc. Nephrol. 4, 817 (Abstract).
Rankin, C.A., Ziemer, D.M., Foo, I. and Calvet, J.P. (1993) Cultured cystic kidney cells from the C57BL/6Jcpk mouse show lower than normal proliferation and increased c-jun gene expression. J. Am. Soc.
Nephrol. 4, 821 (Abstract).
Gattone, V.H., Kuenstler, K.A., Lindemann, G.W. and Calvet, J.P. (1994) Renal expression of a
transforming growth factor-alpha (TGFα) transgene accelerates the progression of inherited
polycystic kidney disease (PKD). J. Am. Soc. Nephrol. 5, 622 (Abstract).
Hou, X., Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1994) Identification of a novel gene that is
abnormally expressed in the polycystic kidneys of the cpk mouse. J. Am. Soc. Nephrol. 5, 626
(Abstract).
Rankin, C.A., Suzuki, K., Ziemer, D.M., Calvet, J.P. and Nagase, H. (1994) Abnormally high levels of
MMPs and TIMPs are synthesized by kidney cells cultured from C57BL/6J cpk mice. J. Am. Soc.
Nephrol. 5, 626 (Abstract).
Davidow, C.J., Maser, R.L., Calvet, J.P. and Grantham, J.J. (1995) Cystic fibrosis transmembrane
conductance regulator (CFTR) mediates transepithelial fluid secretion by human ADPKD cyst
epithelium in vitro. J. Am. Soc. Nephrol. 6, 693.
Rankin, C.A., Suzuki, K., Ziemer, D.M., Grantham, J.J., Calvet, J.P. and Nagase, H. (1995) 72-kDa
Gelatinase A (MMP-2) is found in cyst fluid of 32 human patients and three rodent models of PKD.
J. Am. Soc. Nephrol. 6, 707.
Rankin, C.A., Sarras, M., Calvet, J.P. and DeLisle, R. (1995) The murine polycystic kidney disease gene,
cpk, affects pancreatic development. J. Am. Soc. Nephrol. 6, 708.
Maser, R.L. and Calvet, J.P. (1996) Reduced expression of antioxidant and detoxicant enzyme genes in
polycystic kidney disease (PKD). J. Am. Soc. Nephrol. 7, 1617.
Maser, R.L., Vassmer, D., Calvet, J.P. and Magenheimer, B.S. (1997) Early induction of heme oxygenase1 (HO-1) in polycystic kidney disease (PKD). J. Am. Soc. Nephrol. 8, 376A.
Parnell, S.C. and Calvet, J.P. (1997) Phosphorylation of the PKD1 protein, polycystin-1. J. Am. Soc.
Nephrol. 8, 378A.
Rankin, C.A., Ziemer, D.M., Tian, C., Itoh, Y., Nagase, H., Calvet, J.P. (1997) Matrix metalloproteinase-2
(MMP-2) expression in a murine model of polycystic kidney disease (PKD). J. Am. Soc. Nephrol.
8, 380A.
Maser, R.L., Vassmer, D., Magenheimer, B.S. and Calvet, J.P. (1998) Is induction of heme oxygenase-1
(HO-1) a protective mechanism in polycystic kidney disease (PKD)? J. Am. Soc. Nephrol. 9, 378A.
Parnell, S.C., Magenheimer, B.S., Maser, R.L., Rankin, C.A., Smine, A., Okamoto, T. and Calvet, J.P.
(1998) The polycystic kidney disease-1 protein, polycystin-1, binds and activates heterotrimeric Gproteins. J. Am. Soc. Nephrol. 9, 380A.
Rankin, C.A., Maser, R.L. and Calvet, J.P (1998) Affinity-purified polycystin-1 antibodies for Western
blotting and co-immuno-precipitation analysis. J. Am. Soc. Nephrol. 9, 381A.
Parnell, S.C., Magenheimer, B.S. and Calvet, J.P. (1999) PKA phosphorylation of the polycystin-1 protein.
FASEB J. 13, A1406.
Maser, R.L., Sutters, M., Vassmer, D., Magenheimer, B.S. and Calvet, J.P. (1999) Polycystin-1 augments
glucocorticoid induction of endogenous GST-Ya gene expression in stably transfected M-1 cells. J.
Am. Soc. Nephrol. 10, 420A.
Sutters, M., Maser, R.L., Magenheimer, B.S., Gattone, V.H. and Calvet, J.P. (1999) Inducible expression of
the C-terminal cytoplasmic domain of polycystin-1 in stably transfected M-1 mouse cortical
collecting duct cells. J. Am. Soc. Nephrol. 10, 426A.
Calvet, J.P., Yamaguchi, T., Maser, R.L., Sutters, M. and Grantham, J.J. (2000) A stably transfected Cterminal polycystin-1 construct transforms the cAMP growth-responsive phenotype of M-1 cells. J.
Maser, R.L., Vassmer, D., Magenheimer, B.S. and Calvet, J.P. (2000) Reduced extracellular glutathione
peroxidase enzyme and increased lipid peroxidation levels in polycystic kidney disease (PKD). J.
Parnell, S.C., Magenheimer, B.S., Maser, R.L. and Calvet, J.P. (2000) Polycytin-1 activation of c-Jun-Nterminal kinase and AP-1 is mediated by heterotrimeric G-proteins. J. Am. Soc. Nephrol. 11, 396A.
Parnell, S.C., Magenheimer, B.S., Maser, R.L. and Calvet, J.P. (2000) Polycystin-1 activation of c-Jun Nterminal kinase (JNK) and AP-1 is mediated by heterotrimeric G-proteins. FASEB J. 14, A1343.
Magenheimer, B.S., Rodova, M., Maser, R.L. and Calvet, J.P. (2001) Mutations affecting polycystin-1 Cterminal tail activation of the AP-1 transcription factor. J. Am. Soc. Nephrol. 12, 540A.
Nims, N., Magenheimer, B.S., Vassmer, D., Calvet, J.P. and Maser, R.L. (2001) Transmembrane domain
analysis of polycystin-1. J. Am. Soc. Nephrol. 12, 541A.
Parnell, S.C., Magenheimer, B.S., Maser, R.L. and Calvet, J.P. (2001) Polycytin-1 activation of c-Jun-Nterminal kinase is mediated by Gi2 a nd Gs ubunits of he te rotrime ric G prote ins . J. Am. Soc.
Nephrol. 12, 543A.
Rodova, M., Islam, R., Maser, R.L. and Calvet, J.P. (2001) Initial characterization of the human PKD1
promoter and its induction by 
-catenin. J. Am. Soc. Nephrol. 12, 545A.
Yamaguchi, T., Wallace, D.P., Grantham, J.J. and Calvet, J.P. (2001) Changes in intracellular Ca2+ cause
a phenotypic switch in the proliferative response of M-1 cells to cAMP. J. Am. Soc. Nephrol. 12,
548A.
Hein, R.C., Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (2002) A chimeric polycystin-1 C-tail/PTH
receptor construct constitutively activates AP-1 signaling through heterotrimeric G12/13 family
proteins. J. Am. Soc. Nephrol. 13, 105A.
Magenheimer, B.S., Rodova, M., Slaughter, D.M., Maser, R.L. and Calvet, J.P. (2002) Polycystin-1 C-tail
single amino acid mutations inhibit heterotrimeric G protein signaling. J. Am. Soc. Nephrol. 13,
18A.
Puri, S., Rodova, M., Islam, R., Maser, R.L. and Calvet, J.P. (2002) The human PKD1 promoter is
regulated by Ets family transcription factors. J. Am. Soc. Nephrol. 13, 46A.
Yamaguchi, T., Wallace, D.P., Grantham, J.J. and Calvet, J.P. (2002) Decreased intracellular Ca2+, which
causes an ADPKD-like switch in the proliferative response of M-1 cells to cAMP, is associated with
increased activation of Src, B-Raf, and ERK, and increased B-Raf protein. J. Am. Soc. Nephrol.
13, 105A.
Nims, N., Vassmer, D., Magenheimer, B.S., Calvet, J.P. and Maser, R.L. (2002) Polycystin-1
transmembrane biogenesis. J. Am. Soc. Nephrol. 13, 105A.
Yamaguchi, T., Wallace, D.P., Grantham, J.J., and Calvet, J.P. (2003) Ca 2+ channel blockers transform
normal renal epithelial cells to a cAMP growth-stimulation phenotype, and augment the mitogenic
action of cAMP in ADPKD cyst epthelial cells. J. Am. Soc. Nephrol. 14, 576A.
Puri, S., Magenheimer, B.S., Zien, C.A., Walker, D.D., Maser, R.L., and Calvet, J.P. (2003) The polycystin1 C-tail activates the calcineurin/NFAT pathway in a heterotrimeric G protein-dependent fashion. J.
El-Dahr, S., Dipp, S., Saifudeen, Z., Magenheimer, B.S., Puri, S., Calvet, J. (2004) p53 is a negative
transcriptional regulator of the human polycystic kidney disease-1 (PKD-1) Gene. J. Am. Soc.
Nephrol. 15, 219A.
Puri, S. and Calvet, J.P. (2004) The cyclooxygenase-2 (Cox-2) promoter is a target of polycystin-1
mediated signal transduction. J. Am. Soc. Nephrol. 15, 219A.
Magenheimer, B.S., St. John, P.L., Isom, K.S., Abrahamson, D.R., De Lisle, R.C., Wallace, D.P., Maser,
R.L., Grantham, J.J., and Calvet, J.P. (2004) Cyclic AMP- and CFTR-dependent cystic dilation of
embryonic renal tubules in metanephric organ culture. Am. Soc. Nephrol. 15, 651A.
Alcalay, N.I., Brantley, J.G., Sharma, M., Magenheimer, B.S., Calvet, J.P., and Vanden Heuvel, G.B.
(2004) Ectopic expression of the homeobox gene Cux-1 rescues metanephric growth inhibition by
cyclosporin A. Am. Soc. Nephrol. 15, 675A.
Magenheimer, B.S., Mehzabeen, N., and Calvet, J.P. (2005) Cyclic AMP-dependent ERK activation in
metanephric organ culture in response to Ca2+ restriction and in wild-type vs. mutant Pkd1 kidneys.
J. Am. Soc. Nephrol. 16, 584A.
Xiao, Z.S., Zhang, S.Q., Mahlios, J., Calvet, J.P., Bonewald, L.F., and Quarles, L.D. (2005) Polycystin-1: A
novel mechanosensor in osteoblast/osteocytes coupled to Runx2. J. Am. Soc. Nephrol. 16, 585A.
Yamaguchi, T., Wallace, D.P., and Calvet, J.P. (2005) Inhibition of the calcineurin/NFAT signaling pathway
allows cAMP-dependent stimulation of ERK in normal renal epithelial cells, mimicking the PKD
phenotype. J. Am. Soc. Nephrol. 16, 587A.
Islam, M.R., Puri, S., Rodova, M., Magenheimer, B.S., Maser, R.L., and Calvet, J.P. (2006) Activation of
the polycystic kidney disease-1 (PKD1) promoter by retinoic acid: Involvement of Sp1. J. Am. Soc.
Nephrol. 17, 513A.
Xiao, Z.S., Zhang, S.Q., Mahlios, J., Zhou, G., Magenheimer, B.S., Maser, R.L., Calvet, J.P., Dallas, S.L.,
Guo, D.Y., Bonewald, L.F., and Quarles, L.D. (2006) Primary cilium and polycystin-1 in
osteoblasts/osteocytes and associated abnormalities in skeletogenesis and Runx2 expression. J.
Magenheimer, B.S., Wallace, D.P., Maser, R.L., and Calvet, J.P. (2006) Cyclic AMP-stimulated cyst
formation in heterozygous and homozygous Pkd1-deficient kidneys is dependent on CFTRmediated chloride transport. J. Am. Soc. Nephrol. 17, 89A.
Wallace, D.P., Reif, G.A., Hempson, S.J., Quante, M.T., Calvet, J.P., and Yamaguchi, T. (2006) Periostin
(osteocyte specific factor-2) increases ADPKD cell proliferation through the activation of v-integrin
and 
-catenin/TCF signaling pathways. J. Am. Soc. Nephrol. 17, 703A.
Magenheimer, B.S., Wall, S.M, and Calvet, J.P. (2007) Role of CFTR and NKCC1-dependent fluid
secretion in early cyst formation in mouse embryonic kidneys. J. Am. Soc. Nephrol. 18, 50A.
Xiao, Z., Zhang, S., Magenheimer, B.S., Calvet, J.P., and Quarles, L.D. (2007) Polycystin-1 stimulation of
Runx2-II expression is mediated through the calcium-PI3K-Akt pathway. J. Am. Soc. Nephrol. 18,
127A.
Sundar, S.V., Magenheimer, B.S., and Calvet, J.P. (2007) Tuberin-polycystin-1 interactions and colocalization in primary cilia. J. Am. Soc. Nephrol. 18, 130A.
Nishii, K., Nagaoka, K., Yoshihara, D., Takahashi, H., Kogo, H., Yamada, K., Kowa, H., Yanagihara, I.,
Kurahashi, H., Yamashita, T., Yamaguchi, T., Calvet, J.P., Wallace, D.P., and Nagao, S. (2007)
The mutation in NPHP3 associated with renal cysts in pcy mice disrupts protein interaction between
NPHP3 and NPHP1 in cilia. J. Am. Soc. Nephrol. 18, 359A.
Lu, H., Wallace, D.P., Lei, X., Klaassen, C.D., and Calvet, J.P. (2007) C/EBP, a tra ns cription fa ctor
involved in cell proliferation, is highly expressed in cystic tissue of human ADPKD kidneys. J. Am.
Soc. Nephrol. 18, 778A.
Magenheimer, B.S., Sundar, S.V., Reif, G.A, Wallace, D.P., Maser, R.L., Georg, G.I., Tash, J.S., and
Calvet, J.P. (2008) The Lonidamine Derivative Gamendazole Inhibits Cyst Growth in Pkd1-Deficient
Kidneys in Metanephric Culture by Targeting both Fluid Secretion and Cell Proliferation. J. Am. Soc.
Nephrol. 19, 26A.
Parnell, S.C. and Calvet, J.P. (2008) Protein Phosphatase-1 Inte ra cts with a nd De phos phoryla te s
Polycystin-1. J. Am. Soc. Nephrol. 19, 131A.
Nguyen, A.-N., Wallace, D.P., Magenheimer, B.S., Jansson, K.P., Calvet, J.P., and Blanco, G. (2008)
Ouabain exacerbates cystogenesis in ADPKD via the EGFR-Src-MEK pathway. J. Am. Soc.
Nephrol. 19, 364A.
Sundar, S.V., Magenheimer, B.S., Reif, G.A., Wallace, D.P., Georg, G.I., Tash, J.S., and Calvet, J.P.
(2008) Gamendazole Inhibits ADPKD Cell Proliferation and Induces Cytoskeletal Rearrangements.
J. Am. Soc. Nephrol. 19, 364A.
Parnell, S.C. and Calvet, J.P. (2009) Mutation of the Protein Phosphatase-1 Binding Motif Disrupts
Polycystin-1 Dephosphorylation. J. Am. Soc. Nephrol.
Sundar, S.V., Puri, S., Donnelly, A.C., Blagg, B.S.J., Reif, G.A., Wallace, D.P., and Calvet, J.P. (2009) The
Novel Hsp90 Inhibitor KU−174 Targets the mTOR Pathway in ADPKD Cells and Reduces In Vitro
Cyst Formation. J. Am. Soc. Nephrol.
Shah, D.B., Magenheimer, B.S., Sundar, S.V., Georg, G.I., and Calvet, J.P. (2009) A Potent Lonidamine
Derivative JWS-1-190 Inhibits Cell Proliferation and Cyst Formation in Polycystic Kidney Disease. J.
Am. Soc. Nephrol.
Anuj Gupta, Veena Puri, James P. Calvet and Sanjeev Puri. (2010) E2F family transcription factors
regulate the polycystic kidney disease-1 gene promoter through an Ets binding element. J. Am.
Soc. Nephrol.
Daisuke Yoshihara, Hiroki Kurahashi, Miwa Morita, Masanori Kugita, Yoshiyuki Hiki, Harold M. Aukema,
Tamio Yamaguchi, James P. Calvet, Darren P. Wallace, and Shizuko Nagao. (2010)
Thiazolidinedione Ameliorates Autosomal Recessive Polycystic Kidney and Liver Disease in PCK
Rat, An Orthologous Model of Human ARPKD. J. Am. Soc. Nephrol.
Brenda S. Magenheimer, Shirin Sundar, Dar B. Shah, Sumanth Mulamalla, Gail Reif, Darren P. Wallace,
Joseph S. Tash, Gunda I. Georg and James P. Calvet. (2010) Lonidamine Derivatives Have
Properties Expected of Effective Drugs for Treating ADPKD. J. Am. Soc. Nephrol.
Cibele S. Pinto, Brenda S. Magenheimer, Gail A. Reif, James P. Calvet, Darren P. Wallace. (2010) Role of
phosphodiesterases on cAMP-dependent ERK activation and cyst formation in PKD. J. Am. Soc.
Nephrol.
Katherine Swenson-Fields, Darren P. Wallace, Carolyn J. Vivian, Cibele S. Pinto, Gail A. Reif, Brenda S.
Magenheimer, James P. Calvet, Timothy A. Fields. (2010) Wnt5a is upregulated in ADPKD cyst
epithelial cells and stimulates cAMP production and in vitro cyst growth. J. Am. Soc. Nephrol.
Robin L. Maser, Andreea Chiselita, Brenda S. Magenheimer, James P. Calvet, Donna M. Ziemer, Melissa
Johnson, and Dianne Vassmer. (2010) Curcumin inhibits cAMP-mediated cystic dilation in Pkd1deficient kidneys in metanephric organ culture. J. Am. Soc. Nephrol.
Stephen C. Parnell, Sanjeev Puri, Lance C. Brandenburgh, Darren P. Wallace, and James P. Calvet.
(2010) ADPKD-Associated F4145V Mutation in Polycystin-1 Disrupts its Dephosphorylation by
Protein Phosphatase-1. J. Am. Soc. Nephrol.
Brenda S. Magenheimer, Shirin Sundar, Sumanth Mulamalla, Monica K. Johnson, Gail Reif, Darren P.
Wallace, and James P. Calvet. (2011) Lonidamine Reduces Cyst Size in Polycystic Kidney
Disease. ASN Abstracts 2011.
Daisuke Yoshihara, Masanori Kugita, Hiroki Kurahashi, Miwa Morita, Yoshiyuki Hiki, Tamio Yamaguchi,
Harold M. Aukema, Darren P. Wallace, James P. Calvet, Takafumi Toyohara, Takaaki Abe, and
Shizuko Nagao. (2011) Global Gene Expression Profiling in Kidneys of PPAR- Agonis t-Treated
PCK Rats, an Orthologous Model of Human ARPKD. ASN Abstracts 2011.
Xia Zhou, Lucy X. Fan, Wei Liu, William E. Sweeney, James P. Calvet, Ellis D. Avner, Xiaogang Li. (2011)
Cilia Defects and Polyploidy Linked to Low PC1 Expression in Kidney Epithelia Cells Are Mediated
by Increased Expression of Sirt2 and HDAC6. ASN Abstracts 2011.
Stephen C. Parnell, Brenda S. Magenheimer, Robin L. Maser, Mallory A. Havens, Lynn Magenheimer,
Michelle Hastings, James P. Calvet. (2012) A Single Amino Acid Deletion in the Polycystin-1 (PC1)
C-Tail Affects G-Protein Signaling and Causes PKD in Mice. ASN Abstracts 2012.
Tarundeep Kaur, Nidhi Mahajan, Veena Puri, Vivekanand Jha, Surinder Kumar Singla, James P. Calvet,
Sanjeev Puri. (2012) Transforming Growth Factor Beta: A Molecular Target of Polycystin-1 and
Nuclear Factor of Activated T-Cell Signaling. ASN Abstracts 2012.
Kyle Jansson, Brenda S. Magenheimer, James P. Calvet, Gustavo Blanco. (2012) Over-Expression of the
Polycystin-1 (PC1) C-Tail Enhances the Sensitivity of M-1 Cells to the Effects of Ouabain. ASN
Abstracts 2012.
Patents
DNA Encoding Mouse Phosphotriesterase-Related Protein; Patent # 5552313; Inventors: Calvet, J.P., Hou,
X., Magenheimer, B.S., and Maser, R.L. Issue date: September 3, 1996.
Lonidamine Analogs and Treatment of Polycystic Kidney Disease; Application #12/395,521; Inventors:
Georg, G.I., Tash, J.S., Chakrasali, R., Jakkaraj, S.R., and Calvet, J.P. Filing date: February 27,
2009.
Novobiocin Analogues and Treatment of Polycystic Kidney Disease; Application #12/894,654; Inventors:
Calvet, James, P., Blagg, Brian, S., J., Sundar, Shirin, V., and Magenheimer, Brenda, S. Filing date:
September 30, 2010.
Departmental and Institutional Activities
Participant in Molecular Genetics Symposium, Research Week, October 6, 1983; presented talk, "The
Regulation of Eukaryotic Gene Expression by RNA Processing".
Recruiting Seminar, William Jewell College, October 18, 1983.
Director of Graduate Studies in Biochemistry and Molecular Biology (1984-1986; 1993-1995).
Member, KUMC Institutional Graduate Committee (1984-1986; 1993-1995).
Member, Standing Committee on Graduate Faculty Appointments and Authorizations (1984-1985).
Member, Departmental Immunobiochemist Search Committee (1985).
Member, LCME Subcommittee on Research (1985).
Member, Graduate School Five-Year Review Committee for Biochemistry (1986).
Chairman, Graduate School Five-Year Review Committee for Pathology (1986).
Member, Student Health Policy Committee (1986).
Basic Science Faculty Representative, Medical Class Selection Committee (1988-1990, 1992, 1993; Fall
admissions 1989-1991).
Member, M.D./Ph.D. Advisory Committee (1989-1992).
Member, School of Medicine Academic Committee (1990-1993).
Member, KUMC Graduate Travel Scholarship Committee (1991-1994, 1996-1999).
Chairman, M.D./Ph.D. Academic Review Subcommittee (1991-1992).
Recruiting Seminar, William Jewell College, March 6, 1991.
Judge, KUMC Student Research Forum (1991, 1994-1996, 2006).
Member, KUMC Radiation Safety Committee (1991-1994).
Member, Philip Newmark Award Committee (1992).
Member, Search Committee for Senior Associate Dean for Academic Affairs (1994).
Member, Basic Science Core-Curriculum Feasibility and Planning Committee (19941995).
Chairman, Departmental Search Committee for Molecular Geneticist (recruited Dr. H.
Serizawa) (1995-1996).
Member, IGPBS Modules 3 and 5 Committees (1996-1998).
Course Director, Module 5 “Regulation of Gene Expression and Development” of the
IGPBS (1996).
Member, Search Committee for Chairman of the Department of Microbiology,
Immunology, and Molecular Genetics (recruited Dr. Bill Narayan) (1994-1995;
1997-1999).
Member, Departmental Search Committee for Developmental Molecular Geneticist
(1997-1998) (recruited Dr. Ken Peterson).
Member, Departmental Search Committee for X-Ray Crystallographer (recruited Dr. M.
Sundaramoorthy) (1997-1999).
Chairman, Departmental P&T Committee (1999-2000, 2000-2001).
Member (Dean’s appointment), School of Medicine Promotion and Tenure Committee
(1997-1999).
Instructor, Health Careers Pathways Program (1990-2002).
Member, Chair Review Committee for the Department of Pharmacology, Toxicology, and
Therapeutics, (April/May, 2000).
Presented talk at the Grant Writing Workshop entitled “How to Generate Novel Ideas,”
April 6, 2000.
Mini Medical School presenter, September 26, 2000: “Human Genetic Disease: When
the Blueprint of Life Gets Smudged.”
Member, Search Committee for the Dept. of Molecular and Integrated Physiology
(recruited Dr. Gustavo Blanco) (2000-2001).
Co-Director, Murphy Society (1999-2002).
Chairman, Pathology Course Review Committee (2001-2002).
Member, School of Medicine Research Committee (1993-1996; 1999-2002; 2002-2005,
2006-2009).
Chairman, School of Medicine Research Committee (2001-2002).
Member, Faculty Assembly Research Committee (2001-2002; 2005-2006).
Chairman, Genetics Curriculum Subcommittee of the Education Council (2002-2004).
Member, Chair Review Committee for the Department of Anatomy and Cell Biology
(2004).
Member, Departmental Search Committee for Molecular Geneticist (2004-2006)
(recruited Dr. Joe Fontes).
Co-Chair, LCME Self-Study Committee: Institutional Setting: Academic Setting (20042005).
Member, School of Medicine Faculty Council (1992-1993; 2000-2006).
Member, Biomedical Research Training Program Committee (2005, 2006).
Co-Course Director, Medical Biochemistry 801/802 (2000-2001).
Course Director, Medical Biochemistry 801/802 (2001-2006) (Student Voice Award for
the Best First-Year Course, 2003-2004).
Member, Years 3-4 Curriculum Subcommittee (1995-1997, 2006-2007).
Judge, Capitol Research Summit, Topeka, KS, February 17, 2011.
Interim Director, Kidney Institute (2009-2011) (jointly recruited Dr. Reena Rao and Dr.
Pamela Tran).
Member, Search Committee for Director of the Kidney Institute and Director of the
Division of Nephrology and Hypertension (2009-2010) (recruited Dr. Alan Yu).
Member, Search Committee for Chair of the Dept. of Microbiology, Immunology, and
Molecular Genetics (2010-2011).
Ad Hoc Faculty Investigation and Hearing Committee, 2012.
Chair, Faculty Search Committee for the Department of Cancer Biology (2012) (recruited
Dr. Joan Lewis-Wambi).
Temporary Interim Vice Chancellor for Research and Senior Associate Dean for
Research and Graduate Studies (for Paul Terranova), April 2-16, 2012.
Workshop Presenter, Emotional Maturity in Science. Wednesday, November 28, 2012.
Member, Study Section for Frontiers/Clinical/Lied Pilot Grants. Friday, December 14,
2012.
Judge, Capitol Research Summit, Topeka, KS, February 14, 2013.
Present Activities – Departmental and Institutional
Member, Department of BMB AP&T Committee (1994-present).
Member, Department of BMB Graduate Committee (1983-1986; 1992-1996; 1999present).
Module Director, Genetics & Neoplasia (2006-present).
Member, School of Medicine Phase I Curriculum Committee (2005-present).
Member, School of Medicine M.D./Ph.D. Committee (2008-present).
Co-Leader, Cancer Biology Program, KU Cancer Center (2009-present).
Deputy Director, Kidney Institute, University of Kansas Medical Center (2012-present).
Member, Postdoctoral Advisory/Travel Committee (2012-present).
National/International Activities
Student, Embryology Course and Post-Course Research, Marine Biological Laboratory,
Woods Hole, MA; summer 1971.
Member, Local Arrangements Committee for the 24th annual meeting of the American
Society for Cell Biology, Kansas City, MO, 1984.
Invited Lecturer, Department of Biology, University of Puerto Rico, Rio Piedras, PR,
September 12-23, 1983; presented 6 lectures and conducted 2
laboratory sessions in the graduate course, Modern Topics in Biology (Molecular
Biology).
NIH NIDDK Consultant, "Future Research Strategies Once the Polycystic Kidney
Disease Gene is Isolated", March 20, 1987.
Instructor, Physiology: Cell and Molecular Biology Course, Marine Biological Laboratory,
Woods Hole, MA, July 11- August 6, 1988.
Workshop Speaker, "An Introduction to Molecular Biology for Nephrologists", American
Society of Nephrology, Washington, DC, November 30- December 1, 1989;
presented talks, "Isolation of DNA and RNA" and "cDNA isolation/cDNA
libraries", and conducted two problem-solving sessions.
Invited Symposium Speaker, "Factors Affecting the Progression of Renal Cystic
Disorders", sponsored by the National Kidney Foundation; Washington, DC, Nov.
30, 1990; presented talk, "Mechanisms of Increased Cell Proliferation in Renal
Disease".
Invited Symposium Speaker, "Cell and Molecular Biology of Basement Membranes in
Health and Disease" sponsored by the International Society of Nephrology and
the National Institutes of Health; Airlie, VA, September 19-22, 1991; presented
talk, "Renal Cystic Disease".
Invited Symposium Speaker, "Molecular Genetics of Kidney Diseases" sponsored by the
NIDDK; Reston, VA, March 28-30, 1992; presented talk in session entitled
"Models of Genetic Diseases".
Invited Symposium Speaker, "The Fifth International Workshop on Polycystic Kidney
Disease" sponsored by the Polycystic Kidney Research Foundation; Kansas City,
MO, June 19-20, 1992; presented talk, "Proto-Oncogenes and Abnormalities of
Differentiation in PKD".
Workshop Speaker, "Molecular Approaches to Urologic Diseases" sponsored by the
American Urological Association Office of Education; Houston. TX, August 7-9,
1992; gave the after-dinner (keynote) presentation, entitled "Genes and the
Development of Polycystic Kidney Disease".
Scientific Advisory Board, Polycystic Kidney Disease Foundation, Kansas City, MO
(1993-1999).
Invited Symposium Speaker, "Abnormal Epithelial Cell Proliferation in Renal Cyst
Formation and Growth: The Maturation Arrest Hypothesis"; Forefronts in
Nephrology '94, Molecular Basis of Renal Cystic Disease, sponsored by the
International Society of Nephrology, Niagara-on-the-Lake, Ontario, Canada, May
13-15, 1994.
NIH NIDDK Workshop to Plan the Future of PKD Research; Bethesda, MD; September
19, 1994.
NIH Biochemistry Study Section (Ad Hoc), February 22-24, 1995.
Conference Participant; 6th Annual PKD Conference; sponsored by the Polycystic
Kidney Research Foundation, Washington, DC, June 23-25, 1995; presented talk
entitled “Cell Biology 101".
Invited Grand Rounds Speaker, “Polycystic Kidney Disease”; Southern Illinois University
School of Medicine, Springfield, IL, November 7, 1995.
Invited Symposium Speaker, “Cell Proliferation and Gene Expression Events Following
Acute Renal Injury”; Symposium entitled “Cell Proliferation, Tissue Repair and
Outcome of Toxic Injury”; 16th Annual Meeting of the American College of
Toxicology, Vienna, VA, November 12-15, 1995.
Kidney Research Foundation, Huntington Beach, CA, June 21-23, 1996;
presented talk entitled “Cell Biology 101".
Kidney Research Foundation, Nashville, TN, June 13-15, 1997; presented talk
entitled “Cell Biology 101".
Session Chairman, “Cell Biology of PKD”, 1997 NIDDK Workshop on Polycystic Kidney
Disease, September 10-11, 1997, Crystal City, VA; presented overview for
session and presented a poster.
NIH Special Study Section ZRG4 GRM(07)L, NRSA Postdoctoral Fellowships (F32);
January 12, 1998.
PKRF grant reviews, American Society of Nephrology, Miami Beach, FL, November 4,
1999.
PKRF grant reviews, American Society of Nephrology, Toronto, Canada, October 12,
2000.
External Scientific Advisory Board, Case Western Reserve University School of
Medicine, NIH PKD Center, (1999-2006).
Keynote Speaker at the FASEB Summer Research Conference on “Molecular
Mechanisms & Therapeutic Insights in Polycystic Kidney Disease,” August 10-15,
2002, Tucson, AZ. Keynote address entitled, “Mechanisms of Cystogenesis.”
Also presented a regular session talk entitled, “Transcriptional Regulation of the
PKD1 Gene.”
NIH Special Emphasis Panel ZDK1 GRB-6 (J1), “Program Project Applications in Kidney
Development and PKD,” December 11, 2003, Linthicum, MD.
Invited Symposium Speaker, “Polycystin Signal Transduction”; Symposium entitled
“International Symposium on Polycystic Kidney Disease”; one of 16 international
invited speakers; Shanghai, Peoples' Republic of China, May 23-24, 2004.
Chair, NIH Special Emphasis Panel ZRG1 RUS-D 03, “PKD Science,” teleconference
July 30, 2004.
Invited Discussion Meeting Speaker, “Cell Signalling in Polycystic Cells,” January 20,
2005, London, England.
Invited Speaker at the TSC/PKD Workshop “Polycystic Kidney Disease and Tuberous
Sclerosis Complex: Building Bridges to the Future” March 9-10, 2006, Chicago,
IL. Presented talk “Ca2+ and cAMP signaling in PKD.”
Consultant for Clinical Tools, Inc. for their GeneticSolutions.com curriculum for medical
students (2006-2007).
NIH CMBK Study Section (Ad Hoc), February 12, 2007.
Invited talk “Calcium and cAMP in PKD Cells and Embryonic Kidneys” ISN/Nature
Genetics Forefronts Symposium on Nephrogenetics: From Development to
Physiology” Danvers, MA, March 10, 2007.
Invited talk “Strategies to Inhibit Cyst Formation” Am. Soc. Nephrol. meeting in the
Clinical Nephrology Conference “New Insights, Treatments, and Management
Strategies for ADPKD” San Francisco, CA, November 3, 2007.
Site Visit Grant Reviewer, Fondazione Telethon, Milan, Italy, November 14-15, 2007.
Invited Symposium Speaker, “Cyclic AMP and MAPK Signaling in PKD”; Symposium
entitled “Molecular Targets in Renal Disease”; Bamberg, Germany, October 1719, 2008.
Invited talk “Cellular, In Vitro, and Ex Vivo Models of PKD” Polycystic Kidney Disease
Drug Discovery: Challenges and Opportunities, April 8, 2010, Chicago, Ill.
NIH CMBK (Cellular and Molecular Biology of the Kidney) Study Section (Regular
Member), 2007-2011.
Lillian Jean Kaplan International Prize for Advancement in the Understanding of
Polycystic Kidney Disease awarded jointly by the International Society of
Nephrology and PKD Foundation; presented invited talk “Polycystic Kidney
Disease: A Calcium Signaling Disorder” Vancouver, B.C., April 11, 2011.
John C. Davis Memorial Lecture (keynote address) “Polycystic Kidney Disease: A
Calcium Signaling Disorder” at the Department of Molecular Biosciences
Graduate Student Symposium, University of Kansas, Lawrence, KS, August 17,
2012.
Speaker and Panel Discussant, PKD Webinar Virtual Convention, "What's New in PKD:
A Live Broadcast Day of PKD Learning" Saturday, March 9, 2013.
Present Activities – National/International
Member, External Advisory Committee, P30 PKD Center, Univ. Alabama, Birmingham,
2007-present. (Chair, 2007, 2008, 2011, 2013)
Member, External Advisory Comm., Creighton Univ. Cancer and Smoking Diseases
Research Program – LB595, 2008-present.
Co-organizer, FASEB Summer Research Conference: “Polycystic Kidney Disease, From
Bench to Bedside” Saxtons River, VT, June 26–July 1, 2011.
Co-organizer, FASEB Summer Research Conference: “Polycystic Kidney Disease, From
Bench to Bedside” Lucca, Italy, August 2014.
Member, Award Selection Committee for the Lillian Jean Kaplan International Prize for
Advancement in the Understanding of Polycystic Kidney Disease (2012).
Meetings/Workshops/Conferences Attended
Mini-Symposium on Developmental Biology, Woods Hole, MA, July 7, 1981; presented
invited symposium talk.
West Central States Biochemistry Conference, Lincoln, NE, October 23-24, 1981;
presented platform talk.
American Society for Cell Biology, Anaheim, CA, November 9-13, 1981; presented
platform talk.
RNA Processing, Cold Spring Harbor, NY, May 19-23, 1982.
American Society for Cell Biology, Baltimore, MD, November 30-December 4, 1982;
chaired session entitled "RNA Processing, Ribonucleoprotein", and presented
platform talk.
RNA Processing, Cold Spring Harbor, NY, May 18-22, 1983; presented platform talk.
American Society of Biological Chemists, San Francisco, CA, June 5-9, 1983; presented
poster.
American Society for Cell Biology, San Antonio, TX, November 29-December 3, 1983;
presented poster.
RNA Processing, Cold Spring Harbor, NY, May 16-20, 1984; presented poster.
West Central States Biochemistry Conference, Manhattan, KS, October 26-27, 1984;
presented poster.
American Society for Cell Biology, Kansas City, MO, November 12-16, 1984; presented
two posters.
RNA Processing, Cold Spring Harbor, NY, May 14-18, 1986; presented two posters.
West Central States Biochemistry Conference, Lawrence, KS, October 24-25, 1986;
session chairman.
RNA Processing, Cold Spring Harbor, NY, May 13-17, 1987; presented poster.
Etiology and Pathogenesis of Polycystic Kidney Disease, Deerfield, IL, September 1718, 1987; invited symposium speaker.
American Society for Cell Biology, St. Louis, MO, November 16-20, 1987; presented two
posters.
Workshop on Control of Renal Growth, Bethesda, MD, March 7-8, 1988.
Baxter/PKRF Round Table, Overland Park, KS, September 16-17, 1988; invited
speaker.
American Society for Cell Biology and American Society for Biochemistry and Molecular
Biology, San Francisco, CA, January 29- February 2, 1989; presented two
posters.
American Society of Nephrology, Washington, DC, Nov. 30- Dec. 1, 1989; Workshop
Speaker, "An Introduction to Molecular Biology for Nephrologists," presented
talks, "Isolation of DNA and RNA" and "cDNA isolation/cDNA libraries", and
conducted two problem-solving sessions.
National Kidney Foundation/ American Society of Nephrology, Washington, DC,
November 29- December 3, 1990; presented one talk and was an author on
another presented talk.
International Symposium on Cell and Molecular Biology of Basement Membranes in
Health and Disease, Airlie, VA, September 19-22, 1991; presented one invited
talk and one poster.
American Society of Nephrology, Baltimore, MD, November 17-20, 1991; author on
three presented talks.
NIDDK Workshop "Molecular Genetics of Kidney Diseases", Reston, VA, March 28-30,
1992; presented talk.
The Fifth International Workshop on Polycystic Kidney Disease, Kansas City, MO, June
19-20, 1992; presented talk.
Conference sponsored by the American Urological Association Office of Education;
Houston. TX, August 7-9, 1992; Workshop Speaker, "Molecular Approaches to
Urologic Diseases," gave the after-dinner keynote presentation entitled "Genes
and the Development of Polycystic Kidney Disease."
American Society of Nephrology, Baltimore, MD, November 15-18, 1992; author on one
presented talk and one poster.
American Society of Nephrology, Boston, MA, November 14-17, 1993; author on one
poster.
Forefronts in Nephrology '94, Molecular Basis of Renal Cystic Disease, sponsored by
the International Society of Nephrology, Niagara-on-the-Lake, Ontario, Canada,
May 13-15, 1994; Invited Symposium Speaker, "Abnormal Epithelial Cell
Proliferation in Renal Cyst Formation and Growth: The Maturation Arrest
Hypothesis."
American Society of Nephrology, Orlando, FL, October 26-29, 1994; author on one
presented talk and two posters.
6th Annual PKD Conference; sponsored by the Polycystic Kidney Research Foundation,
Washington, DC, June 23-25, 1995; presented talk entitled “Cell Biology 101."
Grand Rounds, Department of Medicine, Southern Illinois University School of Medicine,
Springfield, IL, November 7, 1995.
American College of Toxicology 16th Annual Meeting, Vienna, VA, November 12-15,
1995; invited symposium speaker.
Huntington Beach, CA, June 21-23, 1996; presented talk entitled “Cell Biology
101."
American Society of Nephrology, New Orleans, LA, November 3-6, 1996; author on one
poster; also attended the Basic Science
Conference: Renal Developmental Biology,
November 6-9, 1996.
Nashville, TN, June 13-15, 1997; presented talk entitled “Cell Biology 101."
1997 NIDDK Workshop on Polycystic Kidney Disease, September 10-11, 1997, Crystal
City, VA; presented overview for session
entitled “Cell Biology of PKD” and presented
a poster.
American Society of Nephrology, San Antonio, TX, November 2-5, 1997; author on three
presentations.
American Society of Nephrology, Philadelphia, PA, October 25-28, 1998; author on three
presentations.
American Society for Biochemistry and Molecular Biology, San Francisco, CA, May 1620, 1999; presented one poster.
Fourth World Congress on Advances in Oncology, and Second International Symposium
on Molecular Medicine, Athens, Greece, October 7-9, 1999; presented invited
talk entitled “Signal transduction mediated by the polycystic kidney disease-1
protein.”
American Society of Nephrology, Miami Beach, FL, November 4-8, 1999; author on
three presentations.
Fifth International Workshop on Polycystic Kidney Disease, Leiden/Noordwijkerhout,
Holland, May 11-12, 2000; presented invited talk entitled “The polycystic kidney
disease protein, polycystin-1, binds and activates heterotrimeric G-proteins in
vitro and in vivo,” and session co-chair.
American Society of Nephrology, Toronto, Canada, October 13-16, 2000; author on
three presentations; presented platform talk.
American Society of Nephrology, San Francisco, CA, October 10-17, 2001; author on
five poster presentations.
FASEB Summer Research Conference on “Molecular Mechanisms & Therapeutic
Insights in Polycystic Kidney Disease,” August 10-15, 2002, Tucson, AZ. Keynote
address entitled, “Mechanisms of Cystogenesis” and a regular session talk
entitled, “Transcriptional Regulation of the PKD1 Gene.”
American Society of Nephrology, Philadelphia, PA, October 30-November 4, 2002;
author on two platform presentations, and three poster presentations.
American Society of Nephrology, San Diego, CA, November 12-17, 2003; author on two
poster presentations, and presented invited symposium talk on Nov. 16th entited,
“Beta-catenin and the Wnt signaling pathway in PKD.”
International Symposium on Polycystic Kidney Disease; one of 16 international invited
speakers; Shanghai, Peoples' Republic of China, May 23-24, 2004; presented
invited symposium talk, “Polycystin Signal Transduction.”
American Society of Nephrology, St. Louis, MO, October 27-November 1, 2005. Author
on four posters.
Novartis Foundation Discussion Meeting, London, England; January 20-21, 2005; gave
invited talk entitled “ Cell Signalling in Polycystic Cells.”
FASEB Summer Research Conference on “New Insights in PKD: Molecular Pathways,
Pathogenic Mechanisms, and Translational Applications” at Saxton’s River, VT,
August 8-11. Moderated one session.
American Society of Nephrology, Philadelphia, PA, November 10-12, 2005. Moderated
two sessions: “Polycystins: From Cell Signaling to Gene Regulation” and “PKD:
Basic Biology.” Author on three posters.
TSC/PKD Workshop “Polycystic Kidney Disease and Tuberous Sclerosis Complex:
Building Bridges to the Future” March 9-10, 2006, Chicago, IL. Presented talk
“Ca2+ and cAMP signaling in PKD.”
American Society of Nephrology, San Diego, CA, November 14-19, 2006. Moderated
one session: “Genetic and Molecular Insights in Renal Cyst Development.”
Author on three posters and on one platform presentation (by Brenda
Magenheimer).
American Society of Nephrology, San Francisco, CA, November 2-5, 2007. Invited
speaker: “Strategies to Inhibit Cyst Formation in ADPKD.” Author on four posters
and one platform presentation (by Brenda Magenheimer).
FASEB Summer Research Conference on “Polycystic Kidney Disease: Basic,
Translational and Clinical Science” at Snowmass Village, CO, July 27 – August
1. Moderated one session.
American Society of Nephrology, Philadelphia, PA, November 4-9, 2008. Author on
three posters and one platform presentation (by Brenda Magenheimer).
2nd International Symposium “Molecular Targets in Renal Disease” Bamberg, Germany,
October 17-19, 2008.
Seminars Presented
Nuclear RNA: The Long and Short of It; Department of Biochemistry, University of
Kansas, Lawrence, KS; November 16, 1982.
Nuclear RNA: Structure-Function Studies; Department of Biochemistry, University of
Kansas Medical Center, Kansas City, KS; April 1, 1983.
Nuclear RNA/RNA Interactions and snRNA Function Analyzed by Psoralen
Cross-linking; Department of Biology, University of Puerto Rico, Rio Piedras, PR;
September 21, 1983.
In Vivo Secondary Structure and Intermolecular Base-Pairing Interactions Involving the
Small Nuclear RNAs Analyzed by Psoralen Cross-linking; Department of
Microbiology and Immunology, Washington University School of Medicine, St.
Louis, MO; October 28, 1983.
Nuclear RNA-RNA Interactions Analyzed by Psoralen Cross-linking; Department of
Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical
Center, Kansas City, KS; February 7, 1984.
The Use of Psoralen for Cross-linking RNA; Department of Internal Medicine, Division of
Dermatology, University of Kansas Medical Center, Kansas City, KS; March 3,
1984.
RNA-RNA Interactions in the HeLa Cell Nucleus Analyzed by Psoralen Cross-linking;
Department of Chemistry, Wichita State University, Wichita, KS; October 17,
1984.
In Vivo Secondary Structure and Intermolecular Base-Pairing Interactions Involving
Small Nuclear RNAs Analyzed by Psoralen Cross-linking; Department of Human
Biological Chemistry and Genetics, The University of Texas Medical Branch,
Galveston, TX; December 7, 1984.
The Use of DNA Probes in Diagnosing Infectious Diseases and in Identifying
Disease-Causing Mutant Genes; Marion Laboratories, Inc., Kansas City, MO;
December 20, 1984.
Interactions Between Small Nuclear RNAs and Precursors of mRNA and rRNA shown
by Cross-linking with Psoralen In Vivo; Department of Biochemistry, Kansas
State University, Manhattan, KS; March 25, 1985.
Interactions Between Small Nuclear RNAs and Precursors of mRNA and rRNA shown
by Cross-linking with Psoralen; School of Basic Life Sciences, University of
Missouri-Kansas City, Kansas City, MO; February 13, 1986.
Proto-Oncogene Expression in Polycystic Kidney Disease; Department of Biochemistry,
University of Kansas Medical Center, Kansas City, KS; October 2, 1987.
Gene Expression After Toxic Renal Injury and in Polycystic Kidney Disease; Department
of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical
Center, Kansas City, KS; November 22, 1988.
of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN; April 4,
1989.
of Physiology and Cell Biology, University of Kansas, Lawrence, KS; November
20, 1989.
Gene Expression Associated with Cell Growth and Differentiation in Polycystic Kidneys;
Department of Anatomy and Cell Biology, University of Kansas Medical Center,
Kansas City, KS; May 16, 1990.
Gene Expression and Cell Differentiation in Polycystic Kidney Disease; Division of
Biology, Kansas State University, Manhattan, KS; February 21, 1992.
Overview of Polycystic Kidney Disease Research at the University of Kansas Medical
Center; Sigma Xi presentation, University of Kansas Medical Center, Kansas
City, KS; November 19, 1992.
Gene Expression and Abnormalities of Differentiation in Polycystic Kidney Disease;
Department of Anatomy and Cell Biology, University of Kansas Medical Center,
Kansas City, KS; January 20, 1993.
Polycystic Kidney Disease: A Disease of Many Genes; Department of Biochemistry and
Molecular Biology, University of Kansas Medical Center, Kansas City, KS;
October 8, 1993.
Gene Expression and Cell Differentiation in Polycystic Kidney Disease; Worcester
Foundation for Experimental Biology, Shrewsbury, MA; December 20, 1993.
Gene Expression and Abnormalities of Differentiation in Polycystic Kidney Disease;
Department of Physiology, University of Kansas Medical Center, Kansas City,
KS; March 14, 1994.
Cell Differentiation and Injury in Polycystic Kidney Disease; Genetics Society, University
of Kansas, Lawrence, KS; April 29, 1994.
Abnormal Transcriptional Cascades in Polycystic Kidneys; Department of Microbiology,
Immunology, and Molecular Genetics, University of Kansas Medical Center,
Kansas City, KS; November 17, 1994.
Molecular Genetics of Polycystic Kidney Disease; University of Kansas, Lawrence, KS;
November 4, 1998.
A Molecular Analysis of Polycystic Kidney Disease; Department of Anatomy and Cell
Biology, University of Kansas Medical Center, Kansas City, KS; December 2,
1999.
Polycystic Kidney Disease: Hints About Protein Function; Department of Molecular and
Integrative Physiology, University of Kansas Medical Center, Kansas City, KS;
November 6, 2000.
Polycystic Kidney Disease: A New Paradigm in Signal Transduction; Department of
Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City,
KS, February 28, 2002.
Calcium and cAMP Signaling in Polycystic Kidney Disease; Boston University School of
Medicine, February 9, 2005.
Calcium and cAMP Signaling in Polycystic Disease; University of Missouri at Kansas
City School of Dental Medicine; Oral Biology, December 13, 2006.
The Central Role of cAMP in Polycystic Kidney Disease: Thoughts on Treatments;
Department of Molecular and Integrative Physiology, University of Kansas
Medical Center, Kansas City, KS; December 17, 2007.
Research Update; Kansas City Chapter, PKD Foundation Educational Seminar, March
29, 2008.
Targeting B-Raf in Polycystic Kidney Disease; Interdisciplinary Medical Research
Conference at Internal Medicine Grand Rounds, University of Kansas Medical
Center, Kansas City, KS; May, 14, 2008.
Cyclic AMP, Calcium, and MAPK Signaling in Polycystic Kidney Disease, Department of
Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN; November 18,
2008.
Strategies to Inhibit Cyst Formation in Polycystic Kidney Disease, Division of Nephrology
and Hypertension, Mayo Clinic, Rochester, MN; November 18, 2008.
Strategies to Inhibit Cyst Formation in Polycystic Kidney Disease, Division of
Nephrology, Medical College of Wisconsin, Milwaukee, WI; February 24, 2009.
30, 2009.
29, 2010.
Polycystic Kidney Disease: A Calcium Disorder Causing Cellular Transformation. The
University of Kansas Cancer Center Seminar Series, February 14, 2012.
Research Update for the PKD Foundation Staff, February 21, 23, 2012.
Research Update for PKDF Volunteers Leadership Conference, Kansas City, MO,
March 4, 2012.
Hot Topics, PKD: Pathogenesis to Therapeutics. Laboratory Animal Resources Staff,
Friday, December 14, 2012.
Faculty, Postdoctorals, and Students Mentored/Trained
a. Faculty:
Carolyn A. Rankin, Ph.D. 1986, Research Assistant Professor of Biochemistry
and Molecular Biology, (10/1/88-6/30/98) (Grants obtained while
associated with my lab: a $10,000 KUCC grant, "Abnormal Protooncogene Expression in Cystic Epithelial Cells", a $12,000 NSF grant,
"Identification and Characterization of a Murine Transcriptional Regulator
Gene", a $20,000 Flossie E. West grant, "Gene Activity During Early
Stages of Transformation", and an NIH grant, "The Role of
Immortalization Related Genes in PKD (DK44859, $473,812 direct costs,
5 years, 3/15/92 - 2/28/97).
Helen B. Lovell, M.D., 1976, Assistant Professor of Pediatrics, (7/1/89-6/30/90)
(Grants obtained while associated with my lab: a $25,000, Department of
Pediatrics Telethon grant, "Activated Proto-oncogenes and Cellular
Transformation in Polycystic Kidney Disease" and a $16,500 Menorah
Medical Center Foundation, Phillip S. Astrowe Trust grant, "Polycystic
Kidney Disease Gene Isolation").
Robin L. Maser, Ph.D. 1988, Research Associate Professor of Biochemistry and
Molecular Biology, (9/1/95-present) (Grants obtained while associated
with my lab: a $25,000 PKRF grant, “Role of Glutathione Peroxidase in
Progression of Polycystic Kidney Disease” and a $50,000 AHA grant,
“Functional Analysis of a Novel, Potential Modifier Gene of Polycystic
Kidney Disease”).
Rafiq Islam, Ph.D., Visiting Faculty (summers 2000-2006), currently Professor
and Chair of Chemistry and Physics at Northwest Missouri State
University, Maryville, MO. (Grant obtained based on work carried out in
my lab: “MEKK1 Downregulates the PKD1 Promoter by a Novel
Mechanism” NIH/NIDDK 1R15 DK069897-01 (R. Islam, P.I.) (J. Calvet,
5% effort) Total Direct Costs: $150,000; 03/15/2005 – 02/28/2008 (3
years).
Kathi Glauner, Ph.D., Research Assistant Professor of Biochemistry and
Molecular Biology (currently a medical resident in the KU School of
Medicine). (Grants obtained while associated with my lab: a $35,000 Lied
Endowment Grant, “Tracking Interactions of the Polycystins” 2/1/051/31/06 (J. Calvet, Sponsor); a $50,000 Pilot & Feasibility Grant, “Defining
Polycystin-1 & -2 Interactions” (a component of the PKD Center grant),
awarded but declined because she left the institution to teach for a year at
the University of Hawaii, Hilo.
Dubravka Matkovic-Calogovic, Ph.D. Fulbright Senior Scholar (J. Calvet,
Sponsor). (She is Professor of Chemistry at the University of Zagreb,
Zagreb, Croatia.) Sept. 2007 to July 2008.
b. Postdoctorals:
Benjamin D. Cowley, Jr., M.D. 1981, Fellow in Nephrology, National Kidney
Foundation Research Fellowship, Research Instructor in Medicine,
(1985-1987); [National Research Council Fellow, NIH, 1987-1989;
currently Professor of Internal Medicine and Director of Nephrology, Univ.
of Oklahoma School of Medicine].
Robin L. Maser, Ph.D. 1988, Postdoctoral Fellow (1988-1993), Research
Associate (1993-1995) (leave of absence 1/21/90-8/31/90 to serve as an
Instructor in the Biology Department, William Jewell College, Liberty,
MO); [currently Assistant Professor of Clinical Laboratory Sciences,
University of Kansas Medical Center].
Michael A. Harding, Ph.D. 1991, Postdoctoral Fellow (1991-1992); [postdoctoral
fellow with Dr. Webster K. Cavenee, Director of the Ludwig Institute,
University of California, San Diego (1992-1997); currently a Research
Assistant Professor, Department of Urology, University of Virginia]
Xiaoying Hou, M.D. 1990, Postdoctoral Fellow (1993-1994); [went to the Univ. of
Alabama, Birmingham and became a postdoctoral fellow in Dr. Lisa
Guay-Woodford’s lab].
Michael Sutters, M.D. 1992, Fellow in Nephrology and Internal Medicine
Resident (1996-1999); [Assistant Professor of Medicine, Johns Hopkins
University School of Medicine. Now in private practice.]
Marianna Rodova, Ph.D. 1983, Research Associate (1998-2002). [currently a
Research Associate at KUMC].
Christopher A. Zien, Ph.D. 2000, Postdoctoral Fellow, (2000-2003). [currently
employed in industry]
Sanjeev Puri, Ph.D. 1994, Research Associate, (2001-2006). [currently on the
faculty in the Department of Biochemistry, Panjab University, Chandigarh,
India.]
Ray Hein, Ph.D. 2002, Postdoctoral Fellow (2001-2003). [currently a laboratory
manager at Clinical Reference Laboratory, Lenexa, KS].
Stephen C. Parnell, Ph.D. 2001, Postdoctoral Fellow (2006-present) [currently a
Research Assistant Professor, KUMC].
Shirin V. Sundar, Ph.D. 2003, Postdoctoral Fellow (2006-2011).
Dar Shah, M.D., Nephrology Fellow (2008-2010).
Sumanth Mulamalla, M.D., Nephrology Fellow (2009-2011).
c. Graduate Students:
Fred L. Smardo, Jr., B.S. 1975, Ph.D. 1985, recipient of the 1985 Philip Newmark
Award; Ph.D. Thesis "Molecular Interactions Between Different Classes of
RNA from the Nucleus and Cytoplasm of Mammalian Cells", (1982-1985);
[attended the University of Kansas School of Medicine, 1985-1989, M.D.
1989; postdoctoral fellow in the National Heart, Lung, and Blood Institute,
NIH, 1989-1991; Resident in medicine at the Washington Hospital Center,
Washington, DC, 1991-1994; Nephrology fellow at the Barnes Hospital,
Washington University School of Medicine, St. Louis, MO, 1994-1996;
[currently a nephrologist in private practice].
Robin L. Maser, B.A. 1980, Ph.D. 1988, Biomedical Sciences Scholar Award,
National Science Foundation Graduate Fellowship, recipient of the 1986
Joe R. Kimmel Research Award; Ph.D. Thesis "Analysis of the Interaction
Between U3 Small Nuclear RNA and Pre-Ribosomal RNA", (1982-1988);
[currently Research Associate Professor of Biochemistry and Molecular
Biology, University of Kansas Medical Center].
Michael A. Harding, B.S. 1982, Ph.D. (honors) 1991, KU Medical Alumni
Distinguished Medical Teaching Fund Scholar Award, Nell A. Kimble
Scholarship Award, recipient of the 1990 Philip Newmark Award, recipient
of the 1990 Joe R. Kimmel Research Award (1983-1992); [postdoctoral
fellow in my lab until 8/92; postdoctoral fellow with Dr. Webster K.
Cavenee, Director of the Ludwig Institute, University of California, San
Diego (1992-1997); currently a Research Assistant Professor with Dan
Theodorescu, Department of Urology, University of Virginia].
Garrett W. Lindemann, B.A. 1985, Ph.D. 1995 (Genetics Program); [postdoctoral
fellow at Microgenics: A Boehringer Mannheim Company, Concord, CA,
then became a Senior Scientist at Microgenics; currently the owner of a
consulting firm, Lindemann Research Consulting, Inc., Big Horn, WY].
Ivy Foo-Hurwitz, B.A. 1986, M.A. 1990, Ph.D. 1999, Kansas Health Foundation
Predoctoral Fellowship; [postdoctoral fellow at Children’s Mercy Hospital,
Kansas City, MO with Dr. Steve Leder; postdoctoral fellow at the
University of Pennsylvania School of Medicine, Philadelphia, PA;
postdoctoral fellow at the U. of NM].
Stephen C. Parnell, B.S. 1995, Ph.D. 2001. Kansas Health Foundation
Predoctoral Fellowship (1995-2001); [postdoctoral fellow with Dr. Henrik
Dohlman, University of North Carolina, Chapel Hill, NC; PKD Foundation
Postdoctoral Fellow in the Kidney Institute, KUMC, currently Research
Assistant Professor in the Department of Biochemistry and Molecular
Biology].
Member, Dissertation Committees: John Ward (Microbiology) (Ph.D. defense, August
20, 1985), Jie Wu (Biochemistry) (Ph.D. defense, June 8, 1988), Lawrence
Fernando (Biochemistry) (Ph.D. defense, July 5, 1989), John Lowe
(Biochemistry) (Ph.D. defense, November 27, 1989), Gregg Schyuler
(Biochemistry) (Ph.D. defense, December 6, 1989), Bernie Beall (Microbiology)
(Ph.D. defense, January 16, 1990), T. Trirawatanapong (Biochemistry) (Ph.D.
defense, July 6, 1990), Kent Dickeson (Biochemistry) (Ph.D. defense, July 17,
1991), Laurie Hanson (Pharmacology) (Ph.D. defense, April 16, 1992), Larry
Dobbs (Biochemistry) (Ph.D. defense, December 15, 1992), Luwen Zhang
(Biochemistry) (Ph.D. defense, March 4, 1993), Sen Xiao (Biochemistry) (Ph.D.
defense, April 20, 1993), Ying Peng (Biochemistry) (Ph.D. defense, November
15, 1995), Mini Kapoor (Biochemistry) (Ph.D. defense, October 20, 1995),
Bingfang Yan (Pharmacology) (Ph.D. defense, January 19, 1995), Brian Jones
(Pharmacology) (Ph.D. defense, April 2, 1996), Luchuan Liang (Biochemistry)
(Ph.D. defense, November 30, 1995), Clarissa Davidow (Pharmacology) (Ph.D.
defense, May 9, 1996), Denise Signorelli (Biochemistry) (Ph.D. defense, June
11, 1996), Kai Fu (Biochemistry) (Ph.D. defense, April 21, 1997), Shihyun You
(Biochemistry) (Ph.D. defense, October 4, 2000), Matt H. Ackermann
(Biochemistry) (Ph.D. defense, October 25, 2001) Gerald Call (Physiology)
(Ph.D. defense, December 4, 2001), Susanna Harju (Biochemistry) (Ph.D.
defense, November 28, 2003), Huimin Jiang (Biochemistry) (Ph.D. defense, April
2, 2004), Yue Xue (Pathology) (Ph.D. defense, June 9, 2004), Ben Weaver
(Biochemistry) (Ph.D. defense, April 3, 2009), Subhash Naik (Biochemistry)
(Ph.D. defense, November 29, 2012), Sally Salah (Anatomy & Cell Biology),
Swathi Iyer (Physiology), Archana Ramon (Physiology), Adam Chazelle
(Biochemistry).
Member, M.A. Thesis Committee for Mitch Litwer (Biochemistry) (M.A. thesis defense,
December 16, 1982), Kirsten Schartzer (Biochemistry) (M.A. thesis defense,
October 22, 1991), Haitao Li (Biochemistry) (M.A. thesis defense, November 27,
1995), Mousheng Xu (Biochemistry) (M.A. thesis defense, January 26, 1996),
Yong Tang (Biochemistry) (M.A. thesis defense, November 25, 1996), Ning Deng
(Pharmacology) (M.S. thesis defense, September 21, 1999), Michelle David
(Biochemistry) (M.S. thesis defense, January 2000), Gregory Bartelma
(Biochemistry) (M.S. thesis defense, June 6, 2001), S. Joshua Langmade
(Biochemistry) (M.S. thesis defense, November 1, 2001), Anna Nunn
(Biochemistry).
Lectures and/or Laboratories Presented in the Following Graduate Courses:
Biochemical Research Techniques: Molecular Genetics
Biochemical Research Techniques: Isotopes and Immunology
Biochemical Research Techniques: Cellular Biochemistry
Theories and Applications of Biochemical Techniques
Advanced Biochemistry
Molecular Genetics
Developmental Biology (Anatomy)
Molecular and Cellular Pharmacology (Pharmacology)
IGPBS Module 3 (Cell and Molecular Biology II)
IGPBS Module 5 (Regulation of Gene Expression and Development)
Carcinogenesis & Cancer Biology (KU Cancer Center)
d. Medical Students:
Kathy R. Gromer, Medical Student Summer Research Traineeship, (summer
1982).
Jonathan G. Wiens, Medical Student Summer Research Traineeship, (summer
1983).
Ellen M. Yetter, employed as student help, (summer, 1990).
Sunny Hwang-Hamilton, employed as student help (spring, 1991).
Charmetra R. Washington, employed as student help (fall 1991 through summer
1992).
Ngoclan T. Nguyen, Medical Student Summer Research Traineeship (with Dr.
Rankin), (summer 1992).
Dann J. Fredrickson, Medical Student Summer Research Traineeship (with Dr.
Rankin), (summer 1992).
Nils R. Hoernle, Medical Student Summer Research Traineeship, (summer 1996)
(First Place - School of Medicine - Student Research Forum)
Joshua Anderson, Medical Student Summer Research Traineeship (summer
2002).
Ikuko Lindsey (Lacheo), Medical Student Summer Research Traineeship
(summer 2003).
Regan DeHart, Medical Student Summer Research Traineeship (summer 2004).
Kyle Jansson, M.D./Ph.D. Student Rotation (summer 2007).
Jennifer Truong, Medical School Research Elective (UTMB) (winter 2009).
e. High School and Undergraduate Students:
Carl A. Foster (summer 1983) Biomedical Student Apprentice Program for
Disadvantaged High School Students (through office of Affirmative Action,
KUMC).
David P. Heitmeyer (recipient of American Society for Microbiology award at 33rd
annual Greater Kansas City Science and Engineering Fair; recipient of
the Kansas City Star Grand Award, American Society for Microbiology
award, Area Medical Council Senior Division 2nd place award, and the
United States Navy: Navy Science Award at the 34th Greater Kansas City
Science and Engineering Fair; recipient of the General Motors Second
Grand Award in Biochemistry, the Patent and Trademark Office award,
and the American Chemical Society fourth place award at the 36th
International Science and Engineering Fair, Shreveport, LA), (1983-1985
as a high school student; summers of 1986 and 1987 as an
undergraduate student); [attended the University of Kansas 1985-1989,
B.A. 1989, Harvard University, Ph.D. (chemistry) 1993; currently involved
in software development for course management at Harvard University
and teaches in their Division of Continuing Education].
Blake L. Anderson (recipient of American Cancer Society award, United States
Air Force Biochemistry award, and Science Pioneers Senior Biological
Individual 3rd place award at the 35th Greater Kansas City Science and
Engineering Fair), (1985-1986).
Amelia M. Calvet employed as student help (summer 1991).
Krista M. Shipley (spring and summer 1992).
Hilary Commack (summers 2000, 2001).
Terry Peterson (summers and vacations 2001-2007).
Monica Johnson (summers and vacations 2006-present).
Gautham Prakash (science fair and school project 2007-2011).
Rayyan Islam (school project 2008-2010).
Jessica Ebner (volunteer student 2009)
Lauren Brumley (volunteer student 2011)
Tyler Stephenson (Baker University graduate, summer 2012)
Comprehensive Exam and Thesis Committees
Chairman, Ph.D. Thesis Committees for Fred L. Smardo (Ph.D. defense, July 12, 1985),
Robin L. Maser (Ph.D. defense, December 8, 1988), Michael A. Harding (Ph.D.
defense, December 19, 1991), Garrett W. Lindemann (Ph.D. defense,
September 15, 1995), Ivy Foo-Hurwitz (Ph.D. defense, June 4, 1999), Stephen
C. Parnell (Ph.D. defense, May 23, 2001).
Member, Ph.D. Thesis Committees for John Ward (Microbiology) (Ph.D. defense,
August 20, 1985), Jie Wu (Biochemistry) (Ph.D. defense, June 8, 1988),
Lawrence Fernando (Biochemistry) (Ph.D. defense, July 5, 1989), John Lowe
(Biochemistry) (Ph.D. defense, November 27, 1989), Gregg Schyuler
(Biochemistry) (Ph.D. defense, December 6, 1989), Bernie Beall (Microbiology)
(Ph.D. defense, January 16, 1990), T. Trirawatanapong (Biochemistry) (Ph.D.
defense, July 6, 1990), Kent Dickeson (Biochemistry) (Ph.D. defense, July 17,
1991), Laurie Hanson (Pharmacology) (Ph.D. defense, April 16, 1992), Larry
Dobbs (Biochemistry) (Ph.D. defense, December 15, 1992), Luwen Zhang
(Biochemistry) (Ph.D. defense, March 4, 1993), Sen Xiao (Biochemistry) (Ph.D.
defense, April 20, 1993), Ying Peng (Biochemistry) (Ph.D. defense, November
15, 1995), Mini Kapoor (Biochemistry) (Ph.D. defense, October 20, 1995),
Bingfang Yan (Pharmacology) (Ph.D. defense, January 19, 1995), Brian Jones
(Pharmacology) (Ph.D. defense, April 2, 1996), Luchuan Liang (Biochemistry)
(Ph.D. defense, November 30, 1995), Clarissa Davidow (Pharmacology) (Ph.D.
defense, May 9, 1996), Denise Signorelli (Biochemistry) (Ph.D. defense, June
11, 1996), Kai Fu (Biochemistry) (Ph.D. defense, April 21, 1997), Shihyun You
(Biochemistry) (Ph.D. defense, October 4, 2000), Matt H. Ackermann
(Biochemistry) (Ph.D. defense, October 25, 2001) Gerald Call (Physiology)
(Ph.D. defense, December 4, 2001), Susanna Harju (Biochemistry) (Ph.D.
defense, November 28, 2003), Huimin Jiang (Biochemistry) (Ph.D. defense, April
2, 2004), Yue Xue (Pathology) (Ph.D. defense, June 9, 2004), Ben Weaver
(Biochemistry) (Ph.D. defense, April 3, 2009), Subhash Naik (Biochemistry)
(Ph.D. defense, November 29, 2012), Sally Salah (Anatomy & Cell Biology),
Swathi Iyer (Physiology), Archana Ramon (Physiology), Adam Chazelle
(Biochemistry).
Member, M.A. Thesis Committee for Mitch Litwer (Biochemistry) (M.A. thesis defense,
December 16, 1982), Kirsten Schartzer (Biochemistry) (M.A. thesis defense,
October 22, 1991), Haitao Li (Biochemistry) (M.A. thesis defense, November 27,
1995), Mousheng Xu (Biochemistry) (M.A. thesis defense, January 26, 1996),
Yong Tang (Biochemistry) (M.A. thesis defense, November 25, 1996), Ning Deng
(Pharmacology) (M.S. thesis defense, September 21, 1999), Michelle David
(Biochemistry) (M.S. thesis defense, January 2000), Gregory Bartelma
(Biochemistry) (M.S. thesis defense, June 6, 2001), S. Joshua Langmade
(Biochemistry) (M.S. thesis defense, November 1, 2001), Anna Nunn
(Biochemistry).
Chairman, Oral Comprehensive Committees for Robin Maser, Michael Harding,
Susanna Harju, Scott Falke.
Member, Oral Comprehensive Committees for Sue Rockenbach (Microbiology), John
Ward (Microbiology), David Dutton (Pharmacology), Larry Dobbs (Biochemistry),
Jie Wu (Biochemistry), Michael Arlotto (Pharmacology), Lawrence Fernando
(Biochemistry), Thaweesak Trirawatanapong (Biochemistry), Bernie Beall
(Microbiology), Laurie Hanson (Pharmacology), Manisha Sahni (Microbiology),
Sen Xiao (Biochemistry), Luwen Zhang (Biochemistry), Peter Bullock
(Pharmacology), Brian Pfeiffer (Microbiology), Ying Peng (Biochemistry), Mini
Kapoor (Biochemistry), Ivy Foo-Hurwitz (Biochemistry), Brian Jones
(Pharmacology), Shihyun You (Biochemistry), Stephen Parnell (Biochemistry),
Alex Dajkovic (Microbiology), Kellie McQueen (Biochemistry), Christina Newport
(Microbiology), Kristina Ollison (Microbiology), Subhash Naik (Biochemistry),
Sally Salah (Anatomy & Cell Biology), Swathi Iyer (Physiology), Archana Ramon
(Physiology), Adam Chazelle (Biochemistry).
Research Personnel
Leslie M. Meyer, B.A., 1980-1981
Edwin D. Oliver, M.S., 1981-1983
Jill A. Myers, B.S., 1981-1983
Cynthia S. Jacob, B.S., 1984-1985
Shirley G. Crist-Orlando, B.S., 1986-1987
Alice W. Lee, B.S., Jan-Nov 1987
Laurie J. Chadwick, M.S., 1987-1990
Kristine K. Cross, B.S., 1988-1989
Wendy A. Shumate, B.A., May-Dec 1988 (on leave from medical school)
Douglas B. Larsen, 1991-1992 (joint supervision with Dr. Helen Lovell)
Dianne Vassmer, B.S. 1997-1999 (joint supervision with Dr. R. Maser)
Frank Kuhn, M.S. 2000-2001
Dana M. Slaughter, M.S. 2001-2002
Nurjahan Mehzabeen, B.S. 2004-2005 (joint supervision with Dr. Kathi Glauner)
Andreea Chisolita, B.S. 2007
Brenda S. Magenheimer, B.A., 1987-present
Monica Johnson, B.A., 2010-2011.
Lynn Magenheimer, M.A., 2012-present
Lectures and/or Laboratories Presented in the Following Courses
Medical Biochemistry 801, 802 (Course Director 2001-2006)
Biochemical Research Techniques: Molecular Genetics
Biochemical Research Techniques: Isotopes and Immunology
Biochemical Research Techniques: Cellular Biochemistry
Theories and Applications of Biochemical Techniques
Advanced Biochemistry
Molecular Genetics
Developmental Biology (Anatomy)
Molecular and Cellular Pharmacology (Pharmacology)
IGPBS Module 3 (Cell and Molecular Biology II)
IGPBS Module 5 (Regulation of Gene Expression and Development)
Foundations in Medicine
Genetics & Neoplasia (Module Director 2006-present)
Carcinogenesis & Cancer Biology (KU Cancer Center)
Funded Grants as Principal Investigator (or P.I. is associated with my lab)
Patterns of Gene Transcription in HeLa Cells; NIH R01 GM27265/30547;
$155,520 (direct costs); 1980-1983.
Patterns of Gene Transcription in HeLa Cells; BRSG; $15,010; 1981-1982.
RNA-RNA Interactions In Vivo Involving Small Nuclear RNAs and Large
Precursors; MACCP; $5,000; 1981-1982.
Nuclear RNA-snRNA Interactions in Mammalian Cells; BRSG; $5,000;
1983-1984.
Nucleolus-Associated Messenger RNA Precursors; Speas Foundation; $19,750;
1984-1985.
Studies on Nucleolar DNA; Fraternal Order of Eagles; $5,000; 1984-1986.
Molecular Genetics of Nucleolar DNA; BRSG; $5,500; 1986.
Cyclic AMP Related Genetic Events in Polycystic Kidney Disease; PKR
Foundation; $25,000; 1989-1990.
Molecular Genetics of Nucleolar DNA; NIH R01 GM36547; $181,616 (approx.
direct costs); 3 years, Sept. 1, 1986 - Aug. 31, 1989 (with one year
extension through Aug 31, 1990).
U3 snRNA-rRNA Interactions; BRSG; $10,000, 1 year, Apr. 1, 1990 - Mar. 31,
1991.
Mechanisms of Cell Proliferation in Polycystic Kidneys; NIH R01 DK37100;
$340,922 (est. direct costs); 4 years, Jan. 1, 1987 - Dec. 31, 1990.
Competitive renewal: Gene Activity in the Development of Polycystic
Kidneys, $549,457 (direct costs); 4 years, Jan. 1, 1991 - Dec. 31, 1994
(with no-cost extension until Dec. 31, 1995).
Role of Glutathione Peroxidase in Progression of Polycystic Kidney Disease;
PKRF; $25,000 (direct costs); 1 year, January 1, 1996 - December 31,
1996; Robin L. Maser, P.I.
Gene Activity and Developmental Regulation in Polycystic Kidney Disease; PKR
Foundation; $50,000; 18 months, May 1, 1990 - Oct. 31, 1991; with
renewal $50,000; 18 months, August 1, 1992 - January 31, 1994; with
renewal , $60,000; 18 months, February 1, 1995 - August 31, 1996.
Renal Development and Injury in Polycystic Kidneys; KUMC Research Institute;
$25,000; 1 year, July 1, 1995 - June 30, 1996.
Polycystin Function in Normal and PKD Cells; PKR Foundation; $75,000; 18
months, May 1, 1997 - September 30, 1998.
Functional Analysis of a Novel, Potential Modifier Gene of Polycystic Kidney
Disease; AHA-KS Affiliate; $49,994 (direct costs); 2 years, July 1, 1996 June 30, 1998; Robin L. Maser, P.I.
Mouse PKD1 Protein and its Biochemical Interactions; NIH R01 DK51047;
$389,349 (direct costs); 4 years, Sept. 29, 1995 - Aug. 31, 1999 (with nocost extension year until Aug. 31, 2000).
The membrane-associated structure of polycystin-1; Polycystic Kidney Research
Foundation (PKRF 98011); $50,000 per year direct costs; January 1,
1999 - December 31, 2001; Robin L. Maser, P.I.
Molecular Mechanisms of Progressive Renal Disorders; NIH P01 DK53763;
$2,736,379 (program project direct costs); 5 years, June 25, 1998 - May
31, 2003; Jared J. Grantham, Program Director; Project 2: Molecular
Mechanisms of Polycystin Function; $841,693 (Project 2 direct costs);
James P. Calvet, P.I.
The PKD1 Promoter is a Target of the Beta-Catenin/TCF Pathway; Lied
Endowed Basic Science Pilot Research Grant, KUMC Research Institute;
2 years, March 21, 2001 - March 20, 2003; $70,000; James P. Calvet, P.I.
Kansas Interdisciplinary Center for PKD Research; NIH P50 DK57301;
$4,258,296 (center total costs); 6 years, Sept. 30, 1999 - Aug. 31, 2005;
Jared J. Grantham, Center Director; Project 3: Polycystin G-protein signal
transduction; $733,405 (Project 3 direct costs); James P. Calvet, P.I.
cAMP-Dependent B-Raf/ERK Activation in a Pkd1 Mouse Model; PKD
Foundation; $117,000 (requested direct costs); 2 years, Jan. 1, 2005 Dec. 31, 2006; James P. Calvet, P.I. (no cost extension year)
B-Raf Inhibition; Deciphera Pharmaceuticals, LLC. $75,000 (direct costs); 1 year
(2008-2009), James P. Calvet, P.I.
Kansas Interdisciplinary Center for PKD Research; NIH P50 DK057301;
$5,512,500 (total costs); 5 years, Sept. 30, 2005 - Aug. 31, 2010; no-cost
extension to Aug. 31, 2011. James P. Calvet, P.I. and Center Director;
Project 2: Polycystin-1 Mediated Calcium and cAMP Signaling; $687,500
(Project 2 direct costs); James P. Calvet, P.I.; Administrative Core,
$125,000 (Core direct costs); James P. Calvet, P.I.
GERALD M. CARLSON
Professor and Chair
The University of Kansas Medical Center
Mail Stop 3030
3901 Rainbow Boulevard
Kansas City, KS 66160
Phone: (913) 588-7005
Fax: (913) 588-7007
E-Mail: [email protected]
Personal Information
Born
September 26, 1946
Marital Status
Sarah (1977)
Married (Susan) with two children: Michael (1970) and
Home Address
4501 Holmes Street, Kansas City, MO 64110-1524
Educational Background
1970 - 1975
Ph.D. in Biochemistry (Enzymology), Iowa State University
Department of Biochemistry and Biophysics,
Ames, IA 50011
1964 – 1969
B.S. in Biochemistry, Washington State University
Department of Chemistry, Pullman, WA 99164
Postdoctoral Training
1975 - 1978
Institute for Enzyme Research, University of Wisconsin
Madison, WI 53705
Academic Appointments
2003 -
Professor and Chair
1997 - 2002
Marion Merrell Dow/Missouri Professor of Structural
Biology and Head
Division of Molecular Biology and Biochemistry
School of Biological Sciences
The University of Missouri – Kansas City
1989 – 1997
Professor and Vice-Chairman
Department of Biochemistry, College of Medicine
The University of Tennessee, Memphis
1986 – 1989
Associate Professor, Department of Biochemistry
College of Medicine, The University of Tennessee,
Memphis
1983 – 1986
Associate Professor, Department of Biochemistry
University of Mississippi Medical Center, Jackson, MS
1978 – 1982
Assistant to Associate Professor, Department of Chemistry
University of South Florida, Tampa, FL
Honors and Awards
J. Biol. Chem. corresponding author paper designated as a “Best of 2012” (2013)
Merton F. Utter Memorial Lecture, Case Western Reserve University School of Medicine
(2012)
UMKC Trustees’ Faculty Fellow Award (2001)
Marion Merrell Dow/Missouri Endowed Professorship in Structural Biology (1997 - 2002)
Student Government Association of U.T. Memphis Excellence in Teaching Award
College of Graduate Health Sciences (1996)
Excellence in Research Award, U.T. Memphis Chapter of Sigma Xi (1992)
Distinguished Alumnus Award, Department of Biochemistry & Biophysics, Iowa State
University (1990)
NIH Research Career Development Award (1983 – 1988)
Outstanding Assistant Professor Award for the College of Natural Sciences
University of South Florida (1982)
Individual NIH-NRSA Postdoctoral Fellowship (1976 – 1977)
Phi Kappa Phi (1973)
NSF Undergraduate Research Fellowship (1968)
Society Memberships
The Biophysical Society (2009)
The Protein Society (1994)
American Chemical Society (1988)
American Society for Biochemistry and Molecular Biology (1982)
Sigma Xi (1977)
President, U.T. Memphis Chapter (1996)
Executive Committee, U.T. Memphis Chapter (1995-1997)
Professional Activities
Editorial Appointments
Editorial Board, Journal of Biochemical and Microbial Technology (2012 - )
Editorial Board, Enzyme Research (2009 - )
Associate Editor, Journal of Biomolecular Techniques (1997 - )
Editorial Board, Journal of Biological Chemistry (1988 - 1993; 1996 - 2000)
Associate Editor, Techniques in Protein Chemistry V-VIII (1994 - 1997),
Academic Press
Society Activities
Federation of American Societies for Experimental Biology (FASEB)
Finance Committee (2002 - 2005)
The Protein Society (PS)
Secretary/Treasurer (2002 - 2005)
Executive Council (1999 - 2006)
Publication Committee (2004 - 2005))
Abstract Programming Committee, Chair (1998 - 2001), Member (1997)
Association of Medical and Graduate Departments of Biochemistry (AMGDB)
Past President (2010)
President (2008, 2009)
President Elect (2007)
Board of Directors (2000 - 2002, 2007 - 2010)
National Caucus of Basic Biomedical Science Chairs (2007 - 2008), Member
American Society for Biochemistry and Molecular Biology (ASBMB)
Public Affairs Advisory Committee (2013 - 2016)
Program Committee for 2001 Annual Meeting (2000)
Miscellaneous National Meetings
Organizer, Annual Meeting of Association of Medical and Graduate Departments
of Biochemistry San Juan, Puerto Rico (2008)
Organizer, “Symposium on the Mechanisms of Cellular Regulation,” Iowa State
University (2000)
Co-organizer, “Biochemical Endocrinology and Signal Transduction Symposium,”
U.T. Memphis (1994)
Grant Review Committees
Canada Foundation for Innovation (Research Hospital Fund Large-scale
Institutional Endeavors)
Member Integrative Review Group (2008)
NIH Macromolecular Structure and Function C Study Section
Member (2007 - 2010)
Temporary Member (2005)
53
University of Missouri Research Board (state-wide system) (2001 - 2002)
NIH Physiological Chemistry Study Section
Member (1989 - 1993)
Special Reviewer (1995)
NIH Biomedical Sciences Study Section
Special Reviewer (1994)
NIH Review Panel for Program Project Grant Applications
University of California, San Diego (2007, 2012)
University of Nebraska Medical Center (1997)
Medical College of Ohio (1986)
University of Cincinnati (1986)
NSF Biochemistry Program Advisory Panel
Member (1985 - 1989)
External Advisory Committee
Protein Structure Function COBRE Grant, University of Kansas - Lawrence (2002
- 2003)
Examination Committees
National Board of Medical Examiners, Biochemistry Committee (1985 - 1989)
External Examiner for Dissertation Defenses
University of Calgary, Biochemistry Department (1996)
University of Miami School of Medicine, Biochemistry Department (1982)
Department and Program Review Committees
Wayne State University, Department of Biochemistry and Molecular Biology
(2013)
Southern Illinois University School of Medicine, entire program of Department of
Microbiology;
Molecular Biology, Microbiology and Biochemistry graduate program (2011)
Visiting Professorships
Pembroke State University, Biology Department
FASEB Visiting Scientist for Minority Institutions Program (1991)
University of South Florida Medical College, Biochemistry Department
54
Series of lectures on carbohydrate metabolism for medical students (1988)
Research Interests
We are studying how communication among subunits of the enzyme phosphorylase
kinase (PhK) regulates its enzymatic activity. PhK, which functions in the cascade
activation of glycogen breakdown, is a particularly attractive system to study regulatory
mechanisms of this type because it is among the largest and most complex enzymes
known. Of its 1.3 million Da mass, 90% has a regulatory role. Through allosteric sites on
its three regulatory subunits, PhK integrates metabolic (ADP), hormonal (cAMP and
Ca2+) and neural (Ca2+) signals, resulting in large changes in its activity. This activity
change in response to diverse physiological signals allows for the tight control of
glycogenolysis and subsequent energy production, e.g., in skeletal muscle PhK
activation by Ca2+ ions couples contraction with energy production to sustain contraction.
We are determining, using a variety of approaches, the mechanisms for how these
different signals alter intersubunit interactions and activity of PhK. Two-hybrid genetic
screening, protein crosslinking coupled with mass spectrometry, and synthetic peptides
are used to identify interacting regions of adjacent subunits. Immunoelectron microscopy
with monoclonal antibodies is used to localize regions of subunits within PhK’s overall
tetrahedral structure. Immunochemistry and chemical modification are used to identify
regions of the protein that are influenced by the allosteric effectors. Finally, site-directed
mutagenesis is used to define interacting residues between subunits and to introduce
reporter groups. The results from these different experimental approaches coalesce to
define the relationships between specific subunit interactions and the control of activity
for this important regulatory enzyme of mammalian energy production.
Research Publications
1. Graves, D.J., Carlson, G.M., Skuster, J.R., Parrish, R.F., Carty, T.J. and Tessmer,
G.W. (1975) Pyridoxal phosphate-dependent conformational states of glycogen
phosphorylase as probed by interconverting enzymes. J. Biol. Chem. 250, 22542258.
2. Carlson, G.M. and Graves, D.J. (1976) Site of action and biphasic effect of neutral
salts in the phosphorylase kinase reaction. Biochemistry 15, 4476-4481.
3. Carlson, G.M. and Graves, D.J. (1976) Stimulation of phosphorylase kinase
autophosphorylation by peptide analogs of phosphorylase. J. Biol. Chem. 251, 74807486.
4. Colombo, G., Carlson, G.M. and Lardy, H.A. (1978) Phosphoenolpyruvate
carboxykinase (guanosine triphosphate) from rat liver cytosol. Separation of
homogenous forms of the enzyme with high and low activity by chromatography on
agarose-hexane-guanosine triphosphate. Biochemistry 17, 5321-5329.
55
5. Carlson, G.M., Colombo, G. and Lardy, H.A. (1978) A vicinal dithiol containing an
essential cysteine in phosphoenolpyruvate carboxykinase (guanosine triphosphate)
from cytosol of rat liver. Biochemistry 17, 5329-5338.
6. Colombo, G., Carlson, G.M. and Lardy, H.A. (1981) Phosphoenolpyruvate
carboxykinase (guanosine 5'-triphosphate) from rat liver cytosol. Dual cation
requirement for the carboxylation reaction. Biochemistry 20, 2749-2757.
7. King, M.M. and Carlson, G.M. (1981) Synergistic effect of Ca2+ and Mg2+ in
promoting an activity of phosphorylase kinase that is insensitive to ethylene glycol
bis (β-aminoethyl ether)-N,N'-tetraacetic acid (EGTA). Arch. Biochem. Biophys. 209,
507-523.
8. King, M.M. and Carlson, G.M. (1981) Interaction of phosphorylase kinase with the
2',3'-dialdehyde derivative of adenosine triphosphate. I. Kinetics of inactivation.
Biochemistry 20, 4382-4387.
9. King, M.M. and Carlson, G.M. (1981) Interaction of phosphorylase kinase with the
2',3'-dialdehyde derivative of adenosine triphosphate. II. Differential inactivation
measured with various protein substrates. Biochemistry 20, 4387-4393.
10. King, M.M. and Carlson, G.M. (1981) Synergistic activation by Ca2+ and Mg2+ as the
primary cause for hysteresis in the phosphorylase kinase reactions. J. Biol. Chem.
256, 11058-11064.
11. King, M.M. and Carlson, G.M. (1982) Affinity labeling of rabbit skeletal muscle
phosphorylase kinase by 5'-(p-fluorosulfonylbenzoyl)adenosine. FEBS Lett. 140,
131-134.
12. King, M.M., Carlson, G.M. and Haley, B.E. (1983) Photoaffinity labeling of the β
Subunit of phosphorylase kinase by 8-azidoadenosine 5'-triphosphate and its 2',3'dialdehyde derivative. J. Biol. Chem. 257, 14058-14068.
13. King, M.M., Fitzgerald, T.J. and Carlson, G.M. (1983) Characterization of initial
autophosphorylation events in rabbit skeletal muscle phosphorylase kinase. J. Biol.
Chem. 258, 9925-9930.
14. Cheng, A. and Carlson, G.M. (1983) Utilization of conical equilibrium dialysis cells to
shorten equilibration time. Anal. Biochem. 134, 505-511.
15. Fitzgerald, T.J. and Carlson, G.M. (1984) Activated states of phosphorylase kinase
as detected by the
chemical crosslinker 1,5-difluoro-2,4-dinitrobenzene. J.
Biol. Chem. 259, 3266-3274.
16. Carlson, G.M. (1984) Precautions when determining kinetically the order of
inactivation of enzymes by functionally irreversible inhibitors. Biochim. Biophys. Acta
789, 347-350.
56
17. Cheng, A., Fitzgerald, T.J. and Carlson, G.M. (1985) Adenosine 5'-diphosphate as
an allosteric effector of phosphorylase kinase from rabbit skeletal muscle. J. Biol.
Chem. 260, 2535-2542.
18. Trempe, M.R., Carlson, G.M., Hainfeld, J.F., Furcinitti, P.S. and Wall, J.S. (1986)
Analyses of phosphorylase kinase by transmission and scanning transmission
electron microscopy. J. Biol. Chem. 261, 2882-2889.
19. Trempe, M.R. and Carlson, G.M. (1987) Phosphorylase kinase conformers: detection
by proteases. J. Biol. Chem. 262, 4333-4340.
20. Fitzgerald, T.J., Trempe, M.R. and Carlson, G.M. (1987) Autophosphorylation of the
α subunit of phosphorylase kinase from rabbit skeletal muscle. J. Biol. Chem. 262,
11239-11246.
21. Paudel, H.K. and Carlson, G.M. (1987) Inhibition of the catalytic subunit of
phosphorylase kinase by its α/β subunits. J. Biol. Chem. 262, 11912-11915.
22. Cheng, A., Fitzgerald, T.J., Bhatnagar, D., Roskoski, R., Jr. and Carlson, G.M.
(1988) Allosteric nucleotide specificity of phosphorylase kinase: correlation of
binding, conformational transitions, and activation. J. Biol. Chem. 263, 5534-5542.
23. Cheng, A. and Carlson, G.M. (1988) Competition between nucleoside diphosphates
and triphosphates at the catalytic and allosteric sites of phosphorylase kinase. J.
Biol. Chem. 263, 5543-5549.
24. Paudel, H.K. and Carlson, G.M. (1988) Renaturation of phosphorylase kinase activity
from sodium dodecyl sulfate-polyacrylamide gels. Arch. Biochem. Biophys. 264, 641646.
25. Lewis, C.T., Seyer, J.M. and Carlson, G.M. (1989) Cysteine-288: an essential,
hyperreactive thiol of cytosolic phosphoenolpyruvate carboxykinase (GTP). J. Biol.
Chem. 264, 27-33.
26. Lewis, C.T., Haley, B.E. and Carlson, G.M. (1989) Formation of an intramolecular
cystine disulfide during the reaction of 8-azido-GTP with cytosolic
phosphoenolpyruvate
carboxykinase
(GTP)
causes
inactivation
without
photolabeling. Biochemistry 28, 9248-9255.
27. Paudel, H.K. and Carlson, G.M. (1990) The quaternary structure of phosphorylase
kinase as influenced by low concentrations of urea: evidence for a structural role for
calmodulin. Biochem. J. 268, 393-399.
28. Paudel, H.K. and Carlson, G.M. (1990) Functional and structural similarities between
the inhibitory region of troponin I coded by exon VII and the calmodulin-binding
regulatory region of the catalytic subunit of phosphorylase kinase. Proc. Natl. Acad.
Sci. U.S.A. 87,7285-7289.
57
29. Paudel, H.K. and Carlson, G.M. (1991) The ATPase activity of phosphorylase kinase
is regulated in parallel with its protein kinase activity. J. Biol. Chem. 266,
16524-16529.
30. Farrar, Y.J.K. and Carlson, G.M. (1991) Kinetic characterization of the
calmodulin-activated catalytic subunit of phosphorylase kinase. Biochemistry 30,
10274-10279.
31. Lewis, C.T., Seyer, J.M. and Carlson, G.M. (1992) Photochemical crosslinking of
guanosine 5’-triphosphate to phosphoenolpyruvate carboxykinase (GTP).
Bioconjugate Chemistry 3, 160-166.
32. Lewis, C.T., Seyer, J.M., Cassell, R.G. and Carlson, G.M. (1993) Identification of
vicinal thiols of phosphoenolpyruvate carboxykinase (GTP). J. Biol. Chem. 268,
1628-1636.
33. Farrar, Y.J.K., Lukas, T.J., Craig, T.A., Watterson, D.M. and Carlson, G.M. (1993)
Features of calmodulin that are important in the activation of the catalytic subunit of
phosphorylase kinase. J. Biol. Chem. 268, 4120-4125.
34. Sanchez, V.E. and Carlson, G.M. (1993) Isolation of an autoinhibitory region from the
regulatory β-subunit of phosphorylase kinase. J. Biol. Chem. 268, 17889-17895.
35. Paudel, H.K., Xu, Y.-H., Jarrett, H.W. and Carlson, G.M. (1993) The model
calmodulin-binding peptide melittin inhibits phosphorylase kinase by interacting with
its catalytic center. Biochemistry 32, 11865-11872.
36. Bender, P.K., Wang, Z. and Carlson, G.M. (1993) Two exons encode the calmodulin
binding domain in the mouse phosphorylase kinase catalytic subunit gene. Genetic
Analysis 10, 99-101.
37. Wilkinson, D.A., Marion, T.N., Tillman, D.M., Norcum, M.T., Hainfeld, J.F., Seyer,
J.M. and Carlson, G.M. (1994) An epitope proximal to the carboxyl terminus of the
α-subunit is located near the lobe tips of the phosphorylase kinase hexadecamer. J.
Mol. Biol. 235, 974-982.
38. Wilkinson, D.A., Tonin, P., Shanske, S., Lombes, A., Carlson, G.M. and DiMauro, S.
(1994) Clinical and biochemical features of ten adult patients with muscle
phosphorylase kinase deficiency. Neurology 44, 461-466.
39. Huang, S., Carlson, G.M. and Cheung, W.Y. (1994) Calmodulin-dependent enzymes
undergo a proton-induced conformational change that is associated with their
interactions with calmodulin. J. Biol. Chem. 269, 7631-7638.
40. Norcum, M.T., Wilkinson, D.A., Carlson, M.C., Hainfeld, J.F. and Carlson, G.M.
(1994) Structure of phosphorylase kinase: a three-dimensional model derived from
stained and unstained electron micrographs. J. Mol. Biol. 241, 94-102.
58
41. Nadeau, O.W. and Carlson, G.M. (1994) Zero-length conformation-dependent crosslinking of phosphorylase kinase subunits by transglutaminase. J. Biol. Chem. 269,
29670-29676.
42. Xu, Y.-H., Wilkinson, D.A. and Carlson, G.M. (1996) Divalent cations but not other
activators enhance phosphorylase kinase’s affinity for glycogen phosphorylase.
43. Willkinson, D.A., Norcum, M.T., Fitzgerald, T.J., Marion, T.N., Tillman, D.M. and
Carlson, G.M. (1997) Proximal regions of the catalytic γ and regulatory β subunits on
the interior lobe face of phosphorylase kinase are structurally coupled to each other
and with enzyme activation. J. Mol. Biol. 265, 319-329.
44. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1997) Differential affinity crosslinking of phosphorylase kinase by the geometric isomers of phenylenedimaleimide.
J. Biol. Chem. 272, 29196-26201.
45. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1997) The structural effects of
endogenous and exogenous Ca2+/calmodulin on phosphorylase kinase. J. Biol.
Chem. 272, 26202-26209.
46. Ayers, N.A., Nadeau, O.W., Read, M.W., Ray, P. and Carlson, G.M. (1998) Effectorsensitive cross-linking of phosphorylase b kinase by the novel crosslinker 4-phenyl1,2,4-triazoline-3,5-dione. Biochem. J. 331, 137-141.
47. Jeyasingham, M.D. and Carlson, G.M. (1998) Evaluation of phosphoenolpyruvate as
a phosphoryl group donor for phosphoproteins in skeletal muscle. Arch. Biochem.
Biophys. 357, 285-292.
48. Nadeau, O.W., Traxler, K.W. and Carlson, G.M. (1998) Zero-length Crosslinking of
the β subunit of phosphorylase kinase to the N-terminal half of its regulatory α
subunit. Biochem. Biophys. Res. Commun. 251, 637-641.
49. Wilkinson, D.A., Fitzgerald, T.J., Marion, T.N. and Carlson, G.M. (1999) Mg2+
induces conformational changes in the γ subunit of phosphorylase kinase, whether
by itself or as part of the holoenzyme complex. J. Prot. Chem. 18, 157-164.
50. Nadeau, O.W., Traxler, K.W., Fee, L.R., Baldwin, B.A. and Carlson, G.M. (1999)
Activators of phosphorylase kinase alter the cross-linking of its catalytic subunit to
the C-terminal one-sixth of its regulatory α subunit. Biochemistry 38, 2551-2559.
51. Xu, Y.-X. and Carlson, G.M. (1999) Structural features contributing to complex
formation between glycogen phosphorylase and phosphorylase kinase. Biochemistry
38, 9562-9569.
52. Ayers, N.A., Wilkinson, D.A., Fitzgerald, T.J. and Carlson, G.M. (1999) Self59
association of the alpha subunit of phosphorylase kinase as determined by twohybrid screening. J. Biol. Chem. 274, 35583-35590.
53. Traxler, K.W., Norcum, M.T., Hainfeld, J.F. and Carlson, G.M. (2001) Direct
visualization of the calmodulin subunit of phosphorylase kinase via subunit exchange
and electron microscopy. J. Struct. Biol. 135, 231-238.
54. Nadeau, O.W., Carlson, G.M. and Gogol, E.P. (2002) A Ca2+-dependent global
conformational change in the 3-D structure of phosphorylase kinase obtained from
electron microscopy. Structure 10, 23-32.
55. Vénien-Bryan, C., Lowe, E.D., Boisset, N., Traxler, K.W., Johnson, L.N. and Carlson,
G.M. (2002) Three-dimensional structure of phosphorylase kinase at 22 Å resolution
and its complex with glycogen phosphorylase b. Structure 10, 33-41.
56. Rice, N.A., Nadeau, O.W., Yang, Q. and Carlson, G.M. (2002) The calmodulinbinding domain of the catalytic
 s ubunit of phos phoryla s e kina s e inte ra c
2+
inhibitory
-sensitive
s ubunit: enetwork
vide nce offorquaternary
a Ca
interactions.
J. Biol. Chem. 277, 14681-14687.
57. Andreeva, I.E., Rice, N.A. and Carlson, G.M. (2002) The regulatory
 s ubunit of
phosphorylase kinase may directly participate in the binding of glycogen
phosphorylase. Biochemistry (Moscow) 67, 1197-1202.
58. Nadeau, O.W., Gogol, E.P. and Carlson, G.M. (2005) Cryoelectron Microscopy
Reveals New Features in the Three-dimensional Structure of Phosphorylase Kinase.
Protein Sci. 14, 914-920.
59. Priddy, T.S., MacDonald, B.A., Heller, W.T., Nadeau, O.W., Trewhella, J. and
Carlson, G.M. (2005)
Ca2+-Induced Structural Changes in Phosphorylase Kinase Detected by Small-angle
X-ray Scattering. Protein Sci. 14, 1039-1048. (Selected for cover)
60. Hilder, T.L., Carlson, G.M., Haystead, T.A.J., Krebs, E.G. and Graves, L.M. (2005)
Caspase-3
Dependent Cleavage and Activation of Skeletal Muscle Phosphorylase b Kinase.
Molec. Cell. Biochem. 275, 233-242.
61. Archila, S., King, M.A., Carlson, G.M. and Rice, N.A. (2006) The Cytoskeletal
Organizing Protein Cdc42-interacting Protein 4 Associates with Phosphorylase
Kinase in Skeletal Muscle. Biochem. Biophys. Res. Commun. 345, 1592-1599.
62. Nadeau, O.W., Anderson, D.W., Yang, Q., Artigues, A., Paschall, J.E., Wyckoff, G.J.,
McClintock, J.L. and Carlson, G.M. (2007) Evidence for the Location of the Allosteric
Activation Switch in the Multisubunit Phosphorylase Kinase Complex from Mass
60
Spectrometric Identification of Chemically Crosslinked Peptides. J. Mol. Biol. 365,
1429-1445.
63. Priddy, T.S., MIddaugh, C.R. and Carlson, G.M. (2007) Electrostatic Changes in
Phosphorylase Kinase Induced by Its Obligatory Allosteric Activator Ca2+. Protein
Sci. 16, 517-527.
64. Priddy, T.S., Price, E.S., Johnson, C.K. and Carlson, G.M. (2007) Single Molecule
Analyses of the Conformational Substates of Calmodulin Bound to the
Phosphorylase Kinase Complex. Protein Sci. 16, 1017-1023.
65. Stiffin, R.S., Sullivan, S.M., Carlson, G.M. and Holyoak, T. (2008) Differential
Inhibition of Cytosolic PEPCK by Substrate Analogues. Kinetic and Structural
Characterization of Inhibitor Recognition. Biochemistry 47, 2099-2109.
66. Jeyasingham, M.D., Artigues, A., Nadeau, O.W. and Carlson, G.M. (2008) Structural
Evidence for Co-evolution of the Regulation of Contraction and Energy Production in
Skeletal Muscle. J. Mol. Biol. 377, 623-629.
67. Nadeau, O.W., Wyckoff, G.J., Paschall, J.E., Artigues, A., Sage, J., Villar, M.T. and
Carlson, G.M. (2008) CrossSearch, a User-friendly Search Engine for Detecting
Chemically Cross-linked Peptides in Conjugated Proteins. Mol. Cell. Proteomics 7,
739-749.
68. Boulatnikov, I.G., Nadeau, O.W., Daniels, P.J., Sage, J.M., Jeyasingham, M.D.,
Villar, M.T., Artigues, A. and Carlson, G.M. (2008) The Regulatory
 S ubunit of
Phosphorylase Kinase Interacts with Glyceraldehyde-3-phosphate Dehydrogenase.
69. Liu, W., Priddy, T.S. and Carlson, G.M. (2008) Physicochemical Changes in
Phosphorylase Kinase Associated with Its Activation. Protein Sci. 17, 2111-2119.
70. Boulatnikov, I.G., Peters, J.L., Nadeau, O.W., Sage, J.M., Daniels, P.J., Kumar, P.,
Walsh, D.A. and Carlson, G.M. (2009) Expressed Phosphorylase b Kinase and Its
 S ubcomple x a s Re gula tory Mode ls for the Ra bbit S ke le ta l Mus cle
Holoenzyme. Biochemistry 48, 10183-10191.
71. Nadeau, O.W., Liu, W., Boulatnikov, I.G., Sage, J.M., Peters, J.L. and Carlson, G.M.
(2010) The Glucoamylase Inhibitor Acarbose Is a Direct Activator of Phosphorylase
Kinase. Biochemistry 49, 6505-6507.
72. Nadeau, O.W., Lane, L.A., Xu, D., Sage, J., Priddy, T.S., Artigues, A., Villar, M.T.,
Yang, Q., Robinson, C.V., Zhang, Y., and Carlson, G.M. (2012) The Structure and
61
Location of the Regulatory  S ubunits in the ()4 Phosphorylase Kinase
Complex. J. Biol. Chem. 287, 36651-36661.
[At publication selected as a Paper of the Week for being “in the top 2 percent
of manuscripts [reviewed] in a year in significance and overall importance”: J.
Biol. Chem. 287, 36662 (2012)]
[In January 2013 selected as one of “only 22 papers – out of more than 4,000
published last year – to receive the designation ‘JBC’s Best of 2012’
(http://www.jbc.org/site/bestoftheyear/)”]
73. Lane, L.A., Nadeau, O.W., Carlson, G.M. and Robinson, C.V. (2012) Mass
Spectrometry Reveals Differences in Stability and Subunit Interactions between
Activated and Nonactivated Conformers of the ( 4 ) Phosphorylase Kinase
Complex. Mol. Cell. Proteomics 11, 1768-1776.
74. Liu, W., Nadeau, O.W. and Carlson, G.M. (2013) Physicochemical Changes in
Phosphorylase Kinase Induced by the Cationic Activator Mg2+. Protein Sci. 22, 444454.
Reviews and Book Chapters
1. Carlson, G.M., Tabatabai, L.B. and Graves, D.J. (1976) The use of an alternative
substrate as a model system for the study of phosphorylase kinase. Metabolic
Interconversion of Enzymes (S. Shaltiel, Ed.), pp. 50-59, Springer-Verlag,
Heidelberg.
2. Carlson, G.M., Bechtel, P.J. and Graves, D.J. (1979) Chemical and regulatory
properties of phosphorylase kinase and cAMP-dependent protein kinase. Advances
in Enzymology 50, 41-115.
3. Carlson, G.M. and Nadeau, O.W. (1999) Bifunctional crosslinking reagents. The
Encyclopedia of Molecular Biology (T.E. Creighton, Ed.), Vol. 1, pp. 279-281, Wiley,
New York.
4. Carlson, G.M. and Nadeau, O.W. (1999) Crosslinking. The Encyclopedia of
Molecular Biology (T.E. Creighton, Ed.), Vol. 1, pp. 582-584, Wiley, New York.
5. Carlson, G.M. and Nadeau, O.W. (1999) Enzyme immobilization and conjugation.
The Encyclopedia of Molecular Biology (T.E. Creighton, Ed.), Vol. 2, p. 829, Wiley,
New York.
6. Carlson, G.M. and Nadeau, O.W. (1999) Glutaraldehyde. The Encyclopedia of
Molecular Biology (T.E. Creighton, Ed.), Vol. 2, pp. 1019-1020, Wiley, New York.
62
7. Rice, N.A. and Carlson, G.M. (2001) Phosphorylase kinase. The Encyclopedia of
Molecular Medicine (T.E. Creighton, Ed.), Vol. 4, pp. 2487-2490, Wiley, New York.
8. Nadeau, O.W. and Carlson, G.M. (2002) Chemical Cross-linking in Studying Proteinprotein Interactions. Protein-Protein Interactions (E.A. Golemis, Ed.), pp. 75-91, Cold
Spring Harbor Laboratory Press, New York.
9. Nadeau, O.W. and Carlson, G.M. (2005) Protein Interactions Captured by Chemical
Cross-linking.
Protein-protein Interactions: A Molecular Cloning Manual (E.A. Golemis, Ed., 2nd
ed.), pp. 105-127, Cold Spring Harbor Laboratory Press, New York.
10. Carlson, G.M. and Holyoak, T. (2009) Structural Insights into the Mechanism of
Phosphoenolpyruvate Carboxykinase Catalysis. J. Biol. Chem. 284, 27037-27041.
(Invited minireview)
11. Carlson, G.M. (2010) How I Became a Biochemist. IUBMB Life 62, 158-161. (Invited
memoir)
12. Nadeau, O.W. and Carlson, G.M. (2012) Methods for Detecting Structural Changes
in Large Protein
Complexes. Methods in Molecular Biology, Vol. 796 (Allostery: Methods and
Protocols; A.W. Fenton,
Ed.), pp. 117-132, Humana Press, Totowa, NJ.
Extramural Research Support
Below are listed only those grants on which GMC was Principal Investigator. The dollar
amounts listed are for the actual awarded direct costs only. Additional grants not listed
include several shared instrumentation grants, international travel grants, including a
NATO award, administrative supplements from NIH, and competitive intramural grants
from state and local sources.
National Institutes of Health
Phosphorylase kinase: role of autophosphorylation
(R01 AM26591/changed to AM32953 upon moving; years 1-2 of 32953)
1980 – 1983, $147,257 Direct Costs
Structure of phosphorylase kinase (R01 AM32953; years 3-7)
63
1983 – 1988, $362,386 Direct Costs
Activation and conformation of phosphorylase kinase (K04 AM01234)
Research Career Development Award
1983 – 1988, $219,831 Direct Costs
Subunit interactions of phosphorylase kinase (R01 DK32953; years 8-12)
1989 – 1994, $654,292 Direct Costs
1994 – 1999, $797,487 Direct Costs
2000 – 2005, $915,969 Direct Costs
2005 – 2009, $1,042,554 Direct Costs
Proteomics to Screen for a Protein Kinase that Uses PEP (R21 DK072393;
Years 1-2)
2005 – 2007, $275,000 Direct Costs
Subunit interactions of phosphorylase kinase (R56 DK32953; year 26)
2009 – 2010, $333,333 Direct Costs
2010 – 2014, $1,278,906 Direct Costs
National Science Foundation
Mapping the active site of phosphoenolpyruvate carboxykinase (DMB-850311)
1986 – 1990, $134,936 Direct Costs (Did not attempt to renew)
Muscular Dystrophy Association
Autophosphorylation of phosphorylase kinase from skeletal muscle
64
1979 – 1981, $50,043 Direct Costs
Interaction of phosphorylase kinase with adenine nucleotides and calcium and
magnesium ions
1981 – 1983, $26,389 Direct Costs (Did not attempt to renew)
Fellows and Graduate Students Trained
Postdoctoral Associates
Kincaid, Margaret
(2011 - )
Funke, Todd
(2011 - 2012)
Peters, Jennifer
(2007 - 2011)
Liu, Weiya
(2006 - 2012)
Boulatnikov, Igor
(2005 - 2011)
Falke, Scott
(2004)
Daniels, Patrick
(2003 - 2007)
Nadeau, Owen W.
Professor)
(1991 - 1997; 1999 - , Research Assistant
Jeyasingham, Marina D.
Professor)
(1987 - 1991; 1999 - 2009, Research Assistant
Traxler, Kenneth W.
(1994 - 1997; Summers 1998 - 2003)
Yang, Qing
(2000 - 2001)
Andreeva, Iraida E.
(1999 - 2000)
Huang, Shengli
(1997 - 1999)
Fee, Lanette
(1995 - 1997)
Farrar, Young J.K.
(1987-1989)
Paudel, Hemant K.
(1985 - 1988, 1989 - 1991)
Norcum, Mona R. (Trempe) (1984 - 1986)
Graduate Students
Thompson, Jackie
(2013 - )
Rimmer, Mary Ashley
(2012 - )
Priddy, Timothy S.
Ph.D. in 2006
Anderson, David
M.S. in 2002
Rice, Nancy A. (Ayers)
Ph.D. in 1999
Xu, Yihong
Ph.D. in 1994
65
Stiffin, Rose M.
Ph.D. in 1994
Wilkinson, Deborah A.
Ph.D. in 1993
Sanchez, Veronica E.
Ph.D. in 1993
Lewis, Cristina T.
Ph.D. in 1989
Cheng, Alexander
Ph.D. in 1986
Herrera, Julio E.
M.S. in 1986
Foster, Stephen M.
M.S. in 1985
Fitzgerald, Thomas J.
Ph.D. in 1985
King, Marita M.
Ph.D. in 1981
Invited Scientific Presentations
Invited Presentations at International, National and Regional Meetings
"New Frontiers in Enzymology: Enzyme Complexes and Regulation," Plenary
lecture,
Occidental Grand Xcaret Resort, Mexico (2013)
“Enzymes, Coenzymes & Metabolic Pathways” Gordon Conference,
Waterville Valley, NH (2012)
th
“8 International Symposium on Mass Spectrometry in the Health and Life
Sciences:
Molecular and Cellular Proteomics,” San Francisco, CA (2007)
“Strategies for Building Multi-scale Structural Models of Cell Signaling
Complexes,”
Los Alamos/UCSD Workshop, San Diego, CA (2001)
“Symposium on the Mechanisms of Cellular Regulation,” Ames, IA, (2000)
“North Dakota EPSCoR Conference on Protein-Protein Interactions,” Grand
Forks (1998)
Annual Conference of the Association of Medical and Graduate Departments of
Biochemistry,
Belize City, Belize (1998)
ASBMB Fall Symposium on “Structure and Function of Protein Kinases and
Phosphatases,” Keystone, CO (1992)
“12th Enzyme Mechanisms Conference,” San Diego (1991) Talk summarized in
Bioorganic
Chemistry 19, 190-224 (1991)
“Techniques, Advances and Utilization of Base Modified Nucleotide Photoaffinity
Probes,”
Subgroup Meeting at ASBMB/ASCB Joint Meeting, San
Francisco (1989)
FASEB Summer Research Conference on “Protein Kinases,” Saxtons River, VT
(1985)
66
“Between the Hope and the Cure,” Florida West Coast Chapter of Muscular
Dystrophy
Association, Sarasota (1980)
“Report from the Research Front,” Atlanta Chapter of Muscular Dystrophy
Association, Atlanta
(1980)
Invited Seminars at Universities, Institutes and Companies (Since 1990,
Excluding Job Interviews)
2012
Case Western Reserve University, Department of Biochemistry
2010
Wichita State University, Departments of Chemistry and Biological Sciences
Missouri State University, Department of Chemistry
2009
University of Missouri – Kansas City, School of Biological Sciences,
Vanderbilt University, Department of Molecular Physiology and Biophysics
2008
Oklahoma State University, Department of Biochemistry and Molecular Biology
University of California, Santa Barbara, Department of Chemistry
2006
University of Massachusetts Medical School, Dept. Biochemistry & Molecular
Pharmacology
2005
Western Kentucky University, Department of Biology
University of Missouri – Columbia, Department of Biochemistry
2004
University of Missouri – Kansas City, School of Pharmacy
University of Utah, Department of Chemistry
Stowers Institute for Medical Research, Kansas City, MO
2003
University of South Florida, Department of Biochemistry and Molecular
Biology
University of Kentucky, Department of Chemistry
2002
Indiana University School of Medicine, Department of Biochemistry and
Molecular Biology
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish
General Hospital, Montreal, Quebec
2001
1999
University of Kansas, Department of Molecular Biosciences, Lawrence, KS
Ruhr-Universitat Bochum, Institut fur Physiologische Chemie, Bochum,
Germany
1997
University of North Carolina (Chapel Hill), Pharmacology Department
Hexos, Inc., Bothell, WA
University of Kansas Medical Center, Biochemistry Department
67
1996
1995
Kansas State University, Department of Biochemistry
URA CNRS (Tours), France
University of South Florida, Department of Chemistry
1994
University of Mississippi Medical Center, Biochemistry Department
Virginia Polytechnic Institute, Biochemistry and Nutrition Department
Louisiana State University Medical Ctr (Shreveport), Biochemistry &
Molecular Biology Dept.
1993 Indiana University School of Medicine, Department of Biochemistry and
Molecular Biology
1992
Baylor College of Medicine, Biochemistry Department
University of Wyoming, Molecular Biology Department
University of Arkansas for Medical Sciences, Biochemistry and Molecular
Biology Department
1991
Memphis State University, Biology Department
Mississippi State University, Biochemistry and Molecular Biology Department
W. Alton Jones Cell Science Center, Lake Placid
University of Vermont, Department of Biochemistry
1990
Meharry Medical College, Biochemistry Department
Iowa State University, Biochemistry and Biophysics Department
Ohio State University, Biochemistry Program
University of Alberta, Biochemistry Department
University of Calgary, Cell Regulation Department
68
CURRICULUM VITAE
Name:
Joan W. Conaway, Ph.D.
Born:
May 8, 1956
New York, New York
Address:
Stowers Institute for Medical Research
1000 E. 50th Street
Tel: 816-926-4091
[email protected]
Education:
1974-1978
A.B., Bryn Mawr College, in Chemistry and in Biology with honors from
Haverford College
1979-1987
Ph.D., in Cell Biology, Stanford University School of Medicine, with Dr. Roger
Kornberg
Post-Graduate Training:
1987-1988
DNAX Institute Research Fellow, DNAX Research Institute of Molecular and
Cellular Biology, Palo Alto, CA.
Professional Appointments:
2001- Investigator, Stowers Institute for Medical Research
2001-
Professor (Affiliate), Department of Biochemistry and Molecular Biology,
2000
Interim Head, Program in Molecular and Cell Biology, Oklahoma Medical
Research Foundation
1998-2006 Adjunct Professor, Department of Biochemistry and Molecular Biology,
University of Oklahoma Health Sciences Center
69
1997-2001
Associate Investigator, Howard Hughes Medical Institute
1996-2001
Member, Program in Molecular and Cell Biology, Oklahoma Medical Research
Foundation, Oklahoma City, OK.
1993-1996
Associate Member, Program in Molecular and Cell Biology, Oklahoma Medical
Research Foundation, Oklahoma City, OK.
1991-1998
Adjunct Assistant Professor, Department of Biochemistry and Molecular
Biology, University of Oklahoma Health Sciences Center
1989-1993
Assistant Member, Program in Molecular and Cell Biology, Oklahoma Medical
Research Foundation, Oklahoma City, OK.
1988-1989
Research Associate and Lecturer, Clayton Foundation Biochemical Institute,
Department of Chemistry, University of Texas, Austin, TX.
Academic Activities:
Thesis Committees
Biochemistry and Molecular Biology, University of Kansas Medical Center Matt Goering
Ray Camahort
Zhen Zhang (current)
Jackie Thompson (current)
Other KUMC departments
Shachi Bhatt
Kendall Smith
Jason Ross
Wen Tang
Evan Janzen
Todd Bradley
Honors:
1991 Edward L. and Thelma Gaylord Award for Scientific Excellence
1997 ASBMB-Amgen Award
2001 Burroughs-Wellcome Visiting Professorship, Saint Louis University School of
Medicine
2002 Elected Fellow, American Academy of Arts and Sciences
2005 Helen Nelson Distinguished Investigatorship
Advisory Boards
70
Chairperson, Board of Scientific Counselors - Basic Science, National Cancer Institute,
Bethesda, MD,
September 2011 - July 2015
Board of Scientific Counselors - Basic Science, National Cancer Institute, Bethesda, MD,
September 2010 - July 2015
Scientific Advisory Board, Institute for Genetics and Molecular and Cellular Biology
(IGBMC)
Strasbourg, France, September 2010 -
Editorial Boards and Review Committees
Editorial Committee, Annual Review of Biochemistry, January 2007- present
Associate Editor, Journal of Biological Chemistry, September 1999- present
Member, NIH Molecular Biology Study Section, February 1994- June 1998
Ad Hoc Member, NIH Molecular Biology Study Section, February 1993
Editorial Board Member, Journal of Biological Chemistry, July 1993 - July 1998
Other Professional Activities:
American Society for Biochemistry and Molecular Biology Meetings Committee, 2002-2004;
2006-2009 (co-chair, small meetings subcommittee); 2009-2012 (committee chair).
Co-chair, Program Committee for the 2009 National Meeting of the American Society for
Biochemistry and Molecular Biology.
Programming Consultant, 2006 Keystone Symposia Series
Co-organizer, 2005 and 2007 Cold Spring Harbor Ubiquitin Family Meeting
Council, American Society of Biochemistry and Molecular Biology, July 2004-July 2007.
Co-organizer, 2003 and 2005 Cold Spring Harbor Meeting on Mechanisms of Eukaryotic
Transcription.
Co-Chair, Program Committee for the 2002 National Meeting of the American Society for
Biochemistry and Molecular Biology, New Orleans, LA.
Chair, Selection Committee, FASEB Excellence in Science Award, 2001
Co-organizer, 2001 Keystone Symposium “Mechanisms of Eukaryotic Transcriptional
Regulation.”
Member, Program Committee for the 2001 National Meeting of the American Society for
Biochemistry and Molecular Biology, Orlando, FL.
Co-organizer, 1999 ASBMB Fall Symposium “Mechanism and Regulation of Transcription
by RNA Polymerase II”
71
Member, Nominations Committee, American Society for Biochemistry and Molecular
Biology, 1999-2002
Member, Selection Committee, FASEB Excellence in Science Award, 1998 - 2000
Session chair, 1996 Gordon Conference on Molecular Genetics
Session chair, 1993 Gordon Conference on Nucleic Acids
Co-organizer, 1992 Keystone Symposium "Fundamental Mechanisms of Transcription"
Session chair, 1992 Gordon Conference on Nuclear Proteins, Chromatin Structure, and Gene
Expression
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Ohh, M., Takagi, Y., Aso, T., Stebbins, C.E., Pavletich, N.P., Zbar, B., Conaway, R.C., Conaway,
J.W., and Kaelin, W.G. (1999) Synthetic peptides define critical contacts between Elongin C,
Elongin B, and the von Hippel-Lindau tumor suppressor protein. J. Clin. Invest., 104, 1583-1591.
84.
Kamura, T., Conrad, M.N., Yan, Q., Conaway, R.C., Conaway, J.W. (1999) The Rbx1 subunit of
SCF and VHL E3 ubiquitin ligase activates Rub1 modifications of cullins Cdc53 and Cul2. Genes.
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Koth, C.M., Botuyan, M.V., Moreland, R.J., Jansma, D., Conaway, J.W., Conaway, R.C., Chazin,
W.J., Friesen, J.D., Arrowsmith, C.H., and Edwards, A.M. (2000) Elongin from Saccharomyces
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and Hanas. J.S. (2000) Identification of a transcription factor IIIA-interacting protein.
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87.
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Kamura, T., Sato, S., Iwai, K., Czyzk-Kryzeska, M., Conaway, R.C., and Conaway, J.W. (2000)
Activation of HIF1α ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor
complex. Proc. Natl. Acad. Sci. USA, 97, 10430-10435.
89.
Conaway, J.W. and Conaway, R.C. (2000) Light at the end of the channel. Science 288, 632-633.
90.
Kamura, T., Burian, D., Khalili, H., Schmidt, S. L., Sato, S., Liu, W.J., Conrad, M.N., Conaway,
R.C., Conaway, J.W., and Shilatifard, A. (2001) Cloning and characterization of ELL-associated
proteins EAP45 and EAP20: A role for yeast EAP-like proteins in regulation of gene expression by
glucose. J. Biol. Chem. 276, 16528-16533
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Dvir, A., Conaway, J.W., and Conaway, R.C. (2001) The RNA polymerase II basal transcriptional
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137. Charlet-Berguerand, N., Feuerhahn, S., Kong, S.E., Ziserman, H., Conaway, J.W., Conaway, R.C.,
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142. Koutelou, E., Sato, S., Tomomori-Sato, C., Florens, L., Swanson, S.K., Washburn, M.P.,
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143. Mahrour, N., Redwine, W.B., Florens, L., Swanson, S.K., Martin-Brown, S., Bradford, W.D.,
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145. Gottschalk, A.J., Conaway, R.C., and Conaway, J.W. (2008) New clues to actin function in
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146. Yao, T., Song, L., Jin, J., Cai, Y., Takahashi, H., Swanson, S.K., Washburn, M.P., Florens, L.,
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148. Yasukawa, T., Kamura, T., Kitajima, S., Conaway, R.C., Conaway, J.W., and Aso, T. (2008)
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152. Gottschalk, A.J., Timinszky, G., Kong, S.E., Jin, J., Cai, Y., Swanson, S.K., Washburn, M.P.,
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153. Takahashi, H., Martin-Brown, S., Washburn, M.P., Florens, L., Conaway, J.W., Conaway, R.C.
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154. Liu, Y. and Conaway, J.W. (2009) When transcription meets recombination: a lesson from the
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155. Harreman, M., Taschner, M., Sigurdsson, S., Anindya, R., Reid, J., Somesh, B., Kong, S.E., Banks,
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157. Lin, C., Smith, E.R., Takahashi, H., Lai, K.C., Martin-Brown, S., Florens, L., Washburn, M.P.,
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158. Capotosti, F., Guernier, S., Lammers, F., Wairdel, P., Cai, Y., Jin, J., Conaway, J.W.,
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160. Mushegian, A. and Conaway, J.W. (2011) Thematic minireview series: computational
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161. Conaway, R.C. and Conaway, J.W. (2011) Function and regulation of the Mediator complex. Curr.
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163. Conaway, R.C. and Conaway, J.W. (2011) Origins and activity of the Mediator Complex (2011)
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164. Costessi, A., Mahrour, N., Tijchon, E., Stunnenberg, R., Stoel, M.A., Jansen, P.W., Sela, D.,
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165. Conaway, R.C. and Conaway, J.W. Von Hippel-Lindau (VHL) Protein. In Encyclopedia of
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168. Conaway, R.C. and Conaway, J.W. (2012) The Mediator complex and transcription elongation.
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170. Gottschalk, A.J., Trivedi, R.D., Conaway, J.W., and Conaway, R.C. (2012) Activation of the SNF2
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171. Tomomori-Sato, C., Sato, S., Conaway, R.C., and Conaway, J.W. (2013) Immunoaffinity
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174. Sela, D., Conkright, J.J., Chen, L., Gilmore, J., Washburn, M.P., Florens, L., Conaway, R.C.,
Conaway, J.W. (2013) Role for human Mediator subunit MED25 in recruitment of mediator to
promoters by endoplasmic reticulum stress-responsive transcription factor ATF6α. J. Biol. Chem.
288, 26179-26187.
175. Chen, L., Conaway, R.C., Conaway, J.W. (2013) Multiple modes of regulation of the human Ino80
Snf2 ATPase by subunits of the INO80 chromatin remodeling complex. Proc. Natl. Acad. Sci.,
USA, in press.
Invited Presentations
Meetings
1990 Invited speaker, Gordon Conference on Nuclear Proteins, Gene Regulation, and
Chromatin Structure, Tilton, New Hampshire.
1993 Invited speaker, Second Annual Austin Spring Meeting, "The Transcription Machine:
Assembly and Function," Austin, Texas.
1993 Invited speaker, Gordon Conference on Nucleic Acids, New Hampton, New
Hampshire.
1994 Invited speaker, Keystone Symposium on "Basic Aspects of Transcription," Keystone,
Colorado.
1995 Invited speaker, Gordon Conference on Nucleic Acids, New Hampton, New
Hampshire.
1995 Special Lecturer, 10th Asagiri Symposium, Asagiri, Japan.
1996 Invited speaker, Keystone Symposium on Transcription, Taos, New Mexico.
88
1996 Invited speaker, 1996 Gordon Conference on Molecular Genetics, Newport, Rhode
Island
1996 Invited speaker, FASEB Meeting on Transcription, Snowmass, Colorado
1997 Invited speaker, Cold Spring Harbor Meeting on "Mechanisms of Eukaryotic
Transcription," Cold Spring Harbor Laboratories, Cold Spring Harbor, New York.
1997 Invited speaker, Symposium on Basic Mechanisms of Transcription Initiation,
Elongation, and Termination, 17th International Congress of Biochemistry and
Molecular Biology and 1997 Annual Meeting of the American Society for
Biochemistry and Molecular Biology, San Francisco, California.
1997 Invited speaker, American Association for Cancer Research Special Conference on
Transcriptional Control of Proliferation, Differentiation, and Development, Bolton's
Landing, New York
1997 Invited speaker, American Society for Nephrology Symposium on RNA Processing,
San Antonio, Texas
1998 Invited speaker, Keystone Symposium on Transcriptional Mechanisms, Taos, New
Mexico.
1998 Invited speaker, Ben May Cancer Biology Symposium, "Regulation of Gene
Expression and Profileration", Chicago, Illinois
1998 Invited speaker, 63rd Cold Spring Harbor Symposium on Quantitative Biology,
"Mechanisms of Transcription", Cold Spring Harbor, New York
1998 Invited speaker, FASEB Summer Research Conference on "Transcriptional
Regulation during Cell Growth, Differentiation and Development",
Snowmass Colorado.
1998 Invited speaker, EMBL Transcription Meeting, Heidelberg, Germany.
1999 Invited speaker, Keystone Symposium on the Molecular Basis of Cancer
1999 Invited speaker, Sixth Cold Spring Harbor Meeting on Mechanisms of
Eukaryotic Transcription, Cold Spring Harbor, New York
1999 Invited speaker, Jaques Monod Conference on Transcription and Development,
Roscoff, France.
2000 Invited Speaker, FASEB Summer Research Conference on "Transcriptional
Regulation during Cell Growth, Differentiation and Development",
Snowmass Colorado.
2000 Invited Speaker, Molecular Genetics Gordon Conference, Connecticut College, New
London, CT.
2000 Invited speaker, Keystone Symposium on the Molecular Basis of Cancer
2001 Invited speaker, Keystone Symposium on Transcription Mechanisms, Santa Fe, NM
2002 Invited speaker, Seventh Cold Spring Harbor Meeting on Mechanisms of Eukaryotic
Transcription, Cold Spring Harbor, NY
2002 Invited speaker, FASEB Summer Research Conference on “Transcriptional Regulation
during Cell Growth, Differentiation, and Development”, Saxton’s River, VT
2003 Invited speaker, Keystone Symposium on the Enzymology of Chromatin and
Transcription, Santa Fe, NM
89
2003 Invited speaker, NIDDK workshop on “Ubiquitin and Ubiquitin-like Modifications in
Health and Disease. National Institute of Diabetes and Digestive and Kidney
Diseases, Bethesda, MD.
2004 Invited speaker, Workshop on “Ubiquitin in Cancer and in Chronic Diseases.” The
Hebrew University of Jerusalem and the Institute for Advanced Studies, Giv’at Ram,
Jerusalem.
2004 Lecturer on “Regulation of Transcription Initiation and Elongation,” 2004-2007 Cold
Spring Harbor Gene Expression Course, Cold Spring Harbor Laboratory.
During Cell Growth, Differentiation, and Development,” Saxton’s River, VT.
2004 Invited speaker, 6th EMBL Transcription Meeting, Heidelberg, Germany.
2004 Invited speaker, 130th Nobel Symposium (Chemistry) on “Molecular Mechanisms in
Biological Systems,” Tällberg, Dalarna, Sweden.
2004 Keynote speaker, ASBMB Fall Symposium on “Transcriptional Regulation by
Chromatin and RNA Polymerase II,” Granlibakken, Lake Tahoe, CA.
2005 Invited speaker and Discussion Leader, Nucleic Acids Gordon Conference, Newport,
R. I.
2005 Plenary speaker, 7th International Symposium on Mass Spectrometry in the Health and
Life Sciences
2006 Invited speaker, Keystone Symposium on “Nucleic Acid Enzymes,” Taos, NM
2006 Invited speaker, Keystone Symposium on “Regulation of Eukaryotic Transcription:
From Chromatin to mRNA,” Taos, NM
2006 Plenary speaker, Midwest Meeting on Chromatin, Transcription, and Nuclear
Dynamics, Iowas City, IA
During Cell Growth, Differentiation, and Development,” Saxton’s River, VT.
2006 Invited speaker, ASBMB Special Symposium, “Transcriptional Regulation by
Chromatin and RNA Polymerase II,” Kiawah Island, SC.
2007 Invited speaker, Salk Institute, Fondation IPSEN, and Nature Symposium on
Biological Complexity, “Diseases of Transcription,” La Jolla, CA
2007 Speaker, Cold Spring Harbor Meeting on Mechanisms of Eukaryotic Transcription,
Cold Spring Harbor, NY
2007 Invited speaker, Joint Annual Meetings of the Molecular Biology Society of Japan and
Japanese Biochemical Society, Yokohama, Japan
2008 Invited speaker, Keystone Symposium on “Regulatory Mechanisms in Eukaryotic
Transcription,” Keystone, CO.
During Cell Growth, Differentiation, and Development,” Snowmass, CO.
2008 Invited speaker, Benzon Symposium No. 55, “Transcription, chromatin, and disease,”
Copenhagen, Denmark
2008 Invited speaker, 8th EMBL Transcription Meeting, Heidelberg, Germany
90
2009 Invited speaker, “Proteomic characterization of macromolecular complexes involved
in DNA metabolism,” Trieste, Italy
2009 Invited speaker, Keystone Symposium on “Deregulation of transcription in cancer:
Controlling cell fate decisions,” Killarney, Co. Kerry, Ireland.
2009 Invited speaker, Cold Spring Harbor Meeting on Mechanisms of Eukaryotic
Transcription, Cold Spring Harbor, NY
2010 Invited speaker, Keystone Symposium "Dynamics of Eukaryotic Transcription During
Development," Big Sky, Montana
2012 Invited speaker, ASBMB Annual Meeting, San Diego, CA.
2012 Invited speaker, ASBMB Special Symposium "Transcriptional Regulation: Chromatin
and RNA Polymerase II," Snowbird, Utah.
2013 Invited speaker, Japanese Biochemical Society Hokkaido Division, Sapporo, Japan.
2013 Keynote speaker, Transcription Cycle Symposium, Hakone, Japan.
Seminars
1989 DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California.
1989 Department of Biology, Haverford College, Haverford, Pennsylvania.
1991 Department of Microbiology and Immunology, University of Oklahoma Health
Sciences Center, Oklahoma City, Oklahoma.
1992 Department of Molecular Biology and Microbiology, Case Western Reserve
University, Cleveland, Ohio.
1992 Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale,
Illinois.
1992 Department of Botany and Microbiology, University of Oklahoma, Norman,
Oklahoma.
1992 University of Colorado Health Science Center, Denver, Colorado.
1993 Department of Biochemistry, University of Texas Southwestern Medical School,
Dallas, Texas.
1993 Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia.
1993 Department of Molecular and Medical Genetics, University of Toronto, Toronto,
Ontario, Canada.
1994 Department of Biochemistry and Molecular Biology, Texas Tech Health Sciences
Center, Lubbock, Texas
1994 Fred Hutchinson Center for Cancer Research, Seattle, Washington.
1994 DNAX Research Institute for Molecular and Cell Biology, Palo Alto, CA.
1994 Tumor Biology Seminar Series, Stanford University School of Medicine, Stanford,
CA.
1995 Department of Molecular and Cell Biology, University of California, Berkeley, CA.
91
1995 Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston,
TX.
1995 Department of Medicine, Louisiana State University Medical Center, Shreveport, LA.
1995 National Institute of Genetics, Mishima, Japan.
1995 Institute of Medical Sciences, University of Tokyo, Japan.
1995 Dana-Farber Cancer Institute, Boston, MA.
1995 Division of Immunology, Department of Medicine, Cornell Medical Center, New
York, NY.
1996 Cancer Therapy and Research Center, San Antonio, Texas.
1996 Department of Chemistry and Biochemistry, University of Texas, Austin, Texas.
1996 Department of Biochemistry, School of Public Health, Johns Hopkins University, Baltimore, MD.
1996 Laboratory of Molecular Embryology, NICHD, NIH, Bethesda, MD.
1996 Department of Molecular Genetics, Biochemistry, and Microbiology, University of
Cincinnati College of Medicine, Cincinnati, OH.
1996 Department of Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh,
PA.
1996 Eukaryotic Gene Expression Course, Cold Spring Harbor Laboratories, Cold Spring
Harbor, NY.
1996 Tumor Biology Seminar Series, Stanford University School of Medicine
1997 ACCESS Seminar Series, University of California, Los Angeles
1997 Department of Molecular Genetics, M. D. Anderson Center for Cancer Research,
Houston, TX
1997 Huntsman Cancer Institute, University of Utah, Salt Lake City, UT
1997 Department of Pathology, University of Oklahoma Health Science Center, Oklahoma
City, OK
1997 Cleveland Clinic, Cleveland OH.
1997 Banting and Best Institute, University of Toronto, Ontario, Canada
1997 ASBMB-Amgen Award Lecture, San Francisco, CA.
1997 Department of Medicine, Medical College of Georgia, Augusta, GA.
1997 Department of Microbiology, University of Texas-Southwestern Medical Center,
Dallas, TX.
1997 Department of Biochemistry, Texas Tech University, Lubbock, TX.
1997 Department of Biochemistry, University of Kansas Medical Center, Kansas City,
Kansas.
1998 Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva
University, Bronx, NY.
1998 Department of Biochemistry and Molecular Biology, University of Oklahoma Health
Science Center, Oklahoma City, OK.
1998
Dana-Farber Cancer Institute, Boston, MA.
1998 Department of Biochemistry, Duke University School of Medicine, Durham, NC.
92
1999 Noble Foundation, Ardmore, OK.
1999 Department of Biochemistry, Louisiana State University Medical Center, New
Orleans, LA.
1999 Department of Biochemistry, Michigan State University, East Lansing, MI.
1999 Department of Biochemistry, Tufts University School of Medicine, Boston, MA
1999 Department of Biological Sciences, Stanford University, Stanford, CA
1999 Department of Biochemistry, Saint Louis University School of Medicine, Saint Louis,
MO
1999 Department of Tumor Cell Biology, St. Jude Children’s Research Hospital, Memphis,
TN
2000 Department of Biochemistry, Oklahoma State University, Stillwater, OK
2000 Dean’s Distinguished Lecture and Department of Molecular and Cellular Physiology
Seminar Series, University of Cincinnati School of Medicine, Cincinnati, OH
2000 The Salk Institute for Biological Studies, San Diego, CA
2000 Medical Scientist Lecture Series and Department of Biological Chemistry, University
of California, Irvine, CA.
2000 National Cancer Institute, Bethesda, MD
2000 Stowers Institute for Medical Research, Kansas City, MO
2001 Huntsman Cancer Institute, University of Utah, Salt Lake City, UT
2001 Department of Biochemistry, Emory University School of Medicine, Atlanta, GA
2001 Department of Biochemistry, New Jersey Medical School, Newark, NJ
2001 Dean’s Distinguished Lecturer, Medical College of Ohio, Toledo, OH
2001 Burroughs-Wellcome Visting Professorship Lecture, Department of Biochemistry, Saint Louis
University School of Medicine, St. Louis, MO
2001 Distinguished Lecturer, University of Buffalo, Buffalo, NY
2001 Department of Biochemistry and Molecular Biology, University of Kansas Medical
Center, Kansas City, KS
2002 Ontario Cancer Institute, Toronto, Ontario, Canada
2002 Institut de Recherches Cliniques de Montreal, Montreal, Quebec, Canada
2002 Tumor Biology Seminar Series, Stanford University School of Medicine
2002 Department of Biological Sciences, University of Missouri, Columbia, MO
2003 Milton S. Hershey Medical Center College of Medicine, Hershey, PA
2003 Cold Spring Harbor Laboratories, Cold Spring Harbor, NY
2003 University of California, San Francisco, CA
2003 Department of Molecular Biology, Princeton University, Princeton, NJ
2003 Department of Biochemistry, Case Western Reserve University School of Medicine,
Cleveland, OH
2003 Graduate Program in Molecular, Cellular, and Developmental Biology, Ohio State
University, Columbus, OH
93
2004 Department of Biochemistry and Molecular Genetics, University of Illinois at
Chicago, Chicago, IL
2004 Department of Molecular Biosciences, University of Kansas, KS
2004 Cancer Center, St. Louis University, St. Louis, MO.
2005 Eppley Cancer Institute, Universty of Nebraska Medical Center, Omaha, NE
2005 Biochemistry and Molecular Biology Seminar Series, Mayo Clinic College of
Medicine, Rochester, MN
2005 Pennington Biomedical Research Center, Baton Rouge, LA
2005 University of North Carolina Lineberger Comprehensive Cancer Center, Chapel Hill,
NC
2005 Department of Biochemistry and Molecular Biology, University of Missouri,
Columbia, MO
2005 Department of Pharmacology, University of Michigan, Ann Arbor, MI
2006 Department of Biochemistry, Dartmouth Medical School, Hanover, NH
2006 Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, MD
2006 Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago,
IL
2006 Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO
2006 Department of Biochemistry, Vanderbilt University, Nashville, TN
2006 Frontiers in Biology Seminar Series, Biochemistry Department, Stanford University,
Stanford, CA
2006 Institute of Molecular Biology and Biotechnology, Foundation for Research and
Technology, Iraklion, Crete.
2007 Department of Biochemistry and Molecular Biology, University of Kentucky,
Lexington, KY
2007 Osaka University, Osaka, Japan
2007 Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
2008 Department of Biochemistry and Molecular Genetics, University of Virginia
2008 Department of Chemistry and Biotechnology/Life Sciences Seminar, Beadle Center,
University of Nebraska.
2008 Department of Biochemistry and Molecular Biology, University of Oklahoma Health
Sciences Center, Oklahoma City, OK.
2009 Cancer Research UK, Clare Hall Laboratories, South Mimms, United Kingdom
2009 NIH Transcription Factor Interest Group, National Insitutes of Health, Bethesda, MD.
2010 Department of Structural Biology, University of Pittsburgh, Pittsburgh, PA
2010 Department of Biochemistry and Molecular Biology, Colorado State University, Fort
Collins, CO.
2011 "Science at the Edge Seminar Series," Michigan State University, East Lansing, MI
2011 School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO
94
2011 Department of Pharmacology, New York University, New York, NY
2011 Cancer Center, Massachusetts General Hospital, Charlestown, MA
2011 Department of Biochemistry & Molecular Biology, Johns Hopkins Bloomberg School
of Public Health, Baltimore, MD
2012 Department of Biochemistry & Molecular Biology, Oklahoma State University,
Stillwater, OK.
2013 Department of Biochemistry, Duke University School of Medicine, Durham, NC
2013 Tokyo Medical and Dental University, Tokyo, Japan
2013 Department of Developmental and Molecular Biology, Albert Einstein College of
Medicine, Bronx, NY
95
Updated February 15, 2013
MS 3030, 3901 Rainbow Boulevard
Kansas City, Kansas 66160
(913)-588-7033
e-mail: [email protected]
Aron W. Fenton
Education
B.S. Biochemistry and Molecular Biology, Oklahoma State University,
Stillwater, OK, 1993
Ph.D. Biochemistry and Molecular Biology, Oklahoma State University,
Stillwater, OK, 1999 (Dr. James B. Blair, advisor)
Postdoctorate, Biochemistry and Biophysics, Texas A&M University,
College Station, TX (Dr. Gregory D. Reinhart, advisor)
Major Areas of
Interest
Allosteric Regulation of Enzymes
Enzymatic Control of Metabolic Pathways
Protein Structure/Function in Signal Transduction Pathways
Metabolic Engineering of Cyanobacteria to Optimize Alkane (Diesel)
Production
Research
Experience
2010-present: Associate Professor, Department of Biochemistry and
Molecular Biology, The University of Kansas Medical Center, Kansas
City, KS. The Mechanism of allosteric regulation in pyruvate kinase.
2004-2010: Assistant Professor, Department of Biochemistry and
Molecular Biology, The University of Kansas Medical Center, Kansas
City, KS. Thermodynamics of allosteric regulation of pyruvate kinase.
2006: Visiting Scholar, School of Molecular and Microbial Biosciences,
The University of Sydney, Sydney, Australia.
Small angle x-ray
scattering of pyruvate kinase (Dr. Jill Trewhella, host).
1999-2003:
Postdoctoral Research Associate, Department of
Biochemistry and Biophysics, Texas A&M University, College Station,
TX.
Thermodynamics
of
allosteric
regulation
of
E.
coli
phosphofructokinase (Dr. Gregory D. Reinhart, advisor).
96
1994–1999: Graduate Research Associate, Department of Biochemistry
and Molecular Biology, Oklahoma State University, Stillwater, OK.
Allosteric regulation of yeast pyruvate kinase (Dr. James B. Blair,
advisor).
1994: Research Technician, Department of Biochemistry and Molecular
Biology, Oklahoma State University, Stillwater, OK. Hormonal regulation
of mammalian carbohydrate metabolism (Dr. James B. Blair, principle
investigator).
1993: Undergraduate Research Technician, Department of Animal
Science, Oklahoma State University, Stillwater, OK. Satiety factors in
swine (Dr. Archie Clutter, principle investigator).
1992-1993: Special Research Student, Department of Biochemistry and
Molecular Biology, Oklahoma State University, Stillwater, OK.
Components of venom (Dr. George Odell, advisor).
Professional
Memberships and
Honors
Selected to co-organize
Biothermodynamics
the
2013
Gibbs
Conference
in
ACS-Kansas City chapter guest speaker to class of 2012 student
honorees
Outstanding Instructor 2010-2011, The University of Kansas Medical
Center, by graduate student vote
American Chemical Society
Biophysical Society
Protein Society
Oklahoma State University Alumni, Lifetime member
Phi Lambda Upsilon, National Honorary Chemical Society, Lifetime
member
Gamma Sigma Delta, Honor Society of Agriculture, Lifetime member
American Institute of Chemists Award
College of Agriculture Fleming Scholarship
Teaching
Experience
2012: Instructor in Interdisciplinary Graduate Program in Biomedical
Sciences covering topics of ligand binding and enzyme kinetics, The
2009-present: Medical School lecturer covering topics of Energy
Metabolism, Medical Biochemistry, The University of Kansas Medical
Center
2009-present:
Instructor in Interdisciplinary Graduate Program in
Biomedical Sciences covering topics of Energy Metabolism, The
2008: Direct graduate course covering the thermodynamic aspects of
97
allosteric and cooperative regulations. This course was taught as an
independent study.
2006-2009: Develop and maintain an e-learning activity on enzymology
for the use of medical student education, The University of Kansas
Medical Center
2006-present:
Invited topic lecture, “Allosteric regulation.”
University of Kansas-Edwards campus, Kansas City, KS
The
2005-present: Discussion Group Leader, Medical Biochemistry 801/802,
2004-2008:
Instructor and Course Director, Module II Bchm892:
Selected Topics in Cell Metabolism, Interdisciplinary Graduate Program
in Biomedical Sciences, The University of Kansas Medical Center
1994-1999:
Teaching Assistant, Department of Biochemistry and
Molecular Biology, Oklahoma State University, Stillwater, OK
Visiting Scientists
Host
Postdoctoral
Fellow Advisor
Graduate Student
Advisor
David Pedergrass,
2005-2007
The University of Kansas-Edwards Campus
KUMC
Charulata Prasannan
2010-2012
Ph.D.
KUMC
Aileen Alontaga
2008-2010
Ph.D.
KUMC
Lu Chen
2008-Present
Ph.D.
KUMC/Stowers
Ph.D.
KUMC/Stowers
Co-Advisor
Rushi Trivedi
2011-Present
Co-Advisor
Graduate Student
Committee
Member
Mary Ashley Rimmer
2012-Present
Ph.D.
KUMC
John “J.P.” McGinnis
2012-Present
Ph.D.
KUMC/Stowers
Danny Miller
2012-Present
Ph.D.
KUMC/Stowers
Ram Kannan
2012-Present
Ph.D.
KUMC/Stowers
Evan Janzen
2011-Present
Ph.D.
KUMC/Stowers
Dan Parente
2010-Present
Ph.D.
KUMC
Mauricio Vargas-Uribe 2010-Present
Ph.D.
KUMC
2009-2011
Ph.D.
KUMC
Subhashchandra Naik 2008-2013
Ben Deng
Ph.D.
KUMC
Troy Johnson
2008-2012
Ph.D.
KUMC
Ozan Kumru
2008
Ph.D.
KUMC
Qualifying committee only
98
Hiroo Katayama
2006-2012
Ph.D.
Viktor Matskevich
2006
Ph.D.
KUMC
Qualifying committee only
Alex Dajković
2005
Ph.D.
KUMC
Greg Bomhoff
2004-2005
M.S.
KUMC
2012
M.S.
KUMC
Graduate Student
Research Rotation Arjun Ishwar
Advisor
Undergraduate
Student
Researcher
Advisor
KUMC
Nancy Stiles
2011
Ph.D.
KUMC
Cassandra Field
2011
Ph.D.
KUMC
Jason Barnett
2010
Ph.D.
KUMC
Mitchell McGill
2008
Ph.D.
KUMC
Rachel Williams
2005
Ph.D.
KUMC
Coleen Flynn
2005
Ph.D.
KUMC
Andrew Bigley
2003
Ph.D.
Texas A&M
Anand Venkatraman
2001
Ph.D.
Texas A&M
Carrie Lagace
2000
Ph.D.
Texas A&M
Peter Cornish
2000
Ph.D.
Texas A&M
Jun-Sung Lim
1999
M.S.
OSU
Gene Harris
1998
Ph.D.
OSU
Bobby Gramling
1998
Ph.D.
OSU
SoYeon Park
1995
M.S.
OSU
Mellisa Sims
1994
Ph.D.
OSU
Nick Domann
2012- KU-Edwards Research Student, KUMC
present
Wendy Alvarez
2012-
Rockhurst Research Student, KUMC
present
Lin Lau
2012
Creighton University Student, KUMC
Michael Gagliano
2012
Kansas State University Student, KUMC
James Urness
20092012
Jena Skillet
2009
KU-Edwards Research Student, KUMC
Danny Miller
20082011
Kristen Morphew
2008
Johnson County Community College,
KUMC
Julie Nguyen
2008
Gilbert Ako
2008
Tyler Marquart
2007
99
High School
Student
Researcher
Advisor
Khedidja Beldjilali
20062007
Kelly Thuet
20062007
Cynthia Costa
2006
Natasha Kaur
2006
John VanNice
2005
Mathews Athiyal
2005
Melinda Stump
2004
Visiting High School Teacher, KUMC
Gissel McDonald
2004
Visiting High School Teacher, KUMC
Ann Menefee
2002
Research Student, Texas A&M
Keisha Hardeman
2000
Anakletic Laboratory Program in
Sciences, Texas A&M
Justin Turner
2000
Office of Naval Research Program,
Texas A&M
Angela Krueger
1999
Oklahoma Partners in Biological
Science, OSU
George Wiland
1998
Research Student, OSU
Catie Howard
1998
Jeff Frazier
1997
Science, OSU
Colby Fuser
1997
Science, OSU
Caleb Lack
1997
Freshman Scholar, OSU
Amber Coulter
1997
Lori Davis
1996
Wentz Scholar, OSU
Philip Morton
1996
Wentz Scholar, OSU
Windy Ives
1995
Wentz Scholar, OSU
Punsasi Rajakaiuna
1995
John Carment
1994
Sang Lau
2012
ACS Seed High School Student,
KUMC
Christine Shultz
2012
Olathe Biotech High School Student
SMART team protein modeling,
KUMC
Nehal Patel
2012
KUMC
Jennifer Wuelzer
2012
KUMC
100
Bridgette Begot
2012
KUMC
Karla Torres
2010
KUMC
Anndrea Fenton
2010
Olathe Biotech High School Student,
KUMC
Michael Chopade
2009
KUMC
Josh Smith
2009
Spring Hill High School Honors Student,
KUMC
Holly Hrabik
2008
KUMC
Kristen Morphew
2008
KUMC
Irene Wang
20062007
KUMC
Jing Huang
2006
KUMC
Doanh Tran
20042005
KUMC
Publications
1. Ishwar, A., Tang, Q., and Fenton, A.W. (2013) Distinguishing the
interactions in the fructose-1,6-bisphosphate binding site of human liver
pyruvate kinase that contribute to allostery. Submitted.
2. Prasannan, C.B., Villar, M.T., Artigues, A., and Fenton, A.W. (2013)
Identification of regions of rabbit muscle pyruvate kinase important for
allosteric regulation by phenylalanine, detected by H/D exchange mass
spectrometry. Submitted.
3. Holyoak, T., Zhang, B., Deng, J., Tang, Q., Prasannan, C.B., Fenton,
A.W. (2013) Energetic coupling between an oxidizable cysteine and the
phosphorylatable N-terminus of human liver pyruvate kinase.
Biochemistry 52, 466-467. –Featured article on Biochemistry web site.
4. Urness, J.M., Clapp, K.M., Timmons, J.C., Bai, X., Chandrasoma, N.,
Buszek, K.R., and Fenton, A.W. (2013) Distinguishing the chemical
moiety of phosphoenolpyruvate that contributes to allostery in muscle
pyruvate kinase. Biochemistry 52, 1-3.
5. Fenton, A.W. (2012) Identification of allosteric activating drug leads
for human liver pyruvate kinase. Book chapter in Allostery: Methods and
Protocol 796, 369-382 in the Methods in Molecular Biology series.
101
6. Prasannan, C.B., Tang, Q., and Fenton, A.W. (2012) Allosteric
regulation of human liver pyruvate kinase by peptides that mimic the
phosphorylated/dephosphorylated N-terminus. Book chapter in Allostery:
Methods and Protocol 796, 335-349 in the Methods in Molecular Biology
series.
7. Miller, D.E., Prasannan, C.B., Villar, M.T., Fenton, A.W., and
Artigues, A. (2012) HDXAnalyzer: Automated analysis and data
reporting of deuterium/hydrogen exchange mass spectrometry. J Am
Soc Mass Spectrom. 23, 425-429.
8. Prasannan, C.B. Artigues, A. and Fenton, A.W. (2011) Monitoring
allostery in D 2 O: a necessary control in studies using
hydrogen/deuterium-exchange to characterize allosteric regulation Anal
Bioanal Chem, 401, 1083-1086..
9. Alontaga, A.Y., and Fenton, A.W. (2011) Effector Analogues Detect
Varied Allosteric Roles for Conserved protein-effector interactions in
pyruvate kinase isozymes. Biochemistry, 50, 1934-1939.
10. Villar, M.T., Miller, D.E., Fenton, A.W. and Artigues, A. (2010)
SAIDE: A Semi-Automated Interface for Hydrogen/Deuterium Exchange
Mass Spectrometry. Proteomica, 6, 63-69.
11. Fenton, A.W., Johnson, T.A., and Holyoak, T. (2010) The pyruvate
kinase model system, a cautionary tale for the use of osmolyte
perturbations to support conformational equilibria in allostery. Protein
Sci. 19, 1796-800.
12. Fenton, A.W., Williams, R., and Trewhella, J. (2010) Changes in
small-angle X-ray scattering parameters observed upon binding of ligand
to rabbit muscle pyruvate kinase are not correlated with allosteric
transitions. Biochemistry, 49, 7202-7209.
13. Fenton, A.W. and Alontaga, A.Y. (2009) The Impact of Ions on
Allosteric Functions in Human Liver Pyruvate Kinase. Methods in
Enzymology, 466, 83-106.
14. Fenton, A. W., and Reinhart, G. D. (2009) Disentangling the Web of
Allosteric Communication:
Quantification of the Four Unique
Heterotropic Inhibiting Interactions in E. coli Phosphofructokinase.
Biochemistry, 48, 12323-12328.
15. Fenton, A.W. and Tang, Q. (2009) An activating interaction between
the unphosphorylated N-terminus of human liver pyruvate kinase and the
main body of the protein is interrupted by phosphorylation.
102
16. Fenton, A.W. and Hutchinson, M. (2009) The pH dependence of the
allosteric response of human liver pyruvate kinase to fructose-1,6bisphosphate, ATP, and alanine. Archives of Biochem. And Biophys.
484, 16-23.
17. Fenton, A. W. (2008) Allostery: an illustrated definition for the
‘second secret of life’, Trends in Biochem. Sci. 33, 420-425.
18. Williams, R., Holyoak, T., McDonald, G., Gui, C., and Fenton, A. W.
(2006) Differentiating a Ligand’s Chemical Requirements for Allosteric
Interactions from Those for Protein Binding; Phenylalanine Inhibition of
Pyruvate Kinase. Biochemistry 45, 5421-5429
19. Pendergrass, D. C., Williams, R., Blair, J. B., and Fenton, A. W.
Natural Mutations and
(2006) Mining for Allosteric Information:
Positional Sequence Conservation in Pyruvate Kinase. IUMBM Life 58,
31-38.
20. Fenton, A. W., Paricharttanakul, N. M., and Reinhart, G. D. (2004)
Disentangling the Web of Allosteric Communication in a Homotetramer:
Heterotropic Activation in Phosphofructokinase from Escherichia coli.
21. Fenton, A. W. and Reinhart, G. D. (2003) Mechanism of Substrate
Inhibition in Escherichia coli Phosphofructokinase. Biochemistry 42,
12676-12681.
Identification of Substrate Contact Residues Important for the Allosteric
Regulation of Phosphofructokinase from E. coli. Biochemistry 42, 64536459.
Allosteric Inhibition and Activation in E. coli Phosphofructokinase use the
Same Binding Sites but Different Pathways. Biophysical Journal 84,
169a.
24. Fenton, A. W., and Reinhart, G. D. (2002) Isolation of a Single
Activating Interaction in E. coli Phosphofructokinase. Biochemistry 41,
13410-13416.
25. Fenton, A. W., and Reinhart, G. D. (2002) Binding Energy is Not
Conferred by Binding Site Residues Alone; the Binding Site of E. Coli
Phosphofructokinase. Biophysical Journal 82, 329a.
26. Fenton, A. W., and Blair, J. B. (2002) Kinetic and Allosteric
Consequences of Mutations in the Subunit and Domain Interfaces and
the Allosteric Site of Yeast Pyruvate Kinase. Archives of Biochemistry
and Biophysics 397, 28-39. -Cover Art.
103
27. Fenton, A. W., and Reinhart, G. D. (2001) Quantification of Isolated
Heterotropic
Interactions
Contributing
to
Allostery
in
Phosphofructokinase from E. coli. Biophysical Journal 80, 309a.
28. Fenton, A. W., and Reinhart, G. D. (2000) Formation of a Modified
Tetramer of E. coli Phosphofructokinase with Only One Native Active
Site and One Native Allosteric Site. Protein Science 9, 129.
29. Lambdin, J. M., Howell, D. E., Kocan, K. M., Murphey, D. R., Arnold,
D. C., Fenton, A. W., Odell, G. V., and Ownby, C. L. (2000) The
Venomous Hair Structure, Venom and Life Cycle of Lagoa Crispata, A
Puss Caterpillar of Oklahoma. Toxicon 38, 1163-1189.
30. Odell, G. V., Fenton, A. W., Ownby, C. L., Doss, m. P., and Schmidt,
J. O. (1999) The Role of Venom Citrate. Toxicon 37, 407-409.
31. Odell, G. V., Ferry, P. C., Vick, L. M., Fenton, A. W., Decker, L. S.,
Cowell, R. L., Ownby, C. L., and Gutierrez, J. M. (1998) Citrate Inhibition
of Snake Venom Proteases. Toxicon 36, 1801-1806.
32. Fenton, A. W., West, P. R., Odell, G. V., Hudiburg, S. M., Ownby, C.
L., Mills, J. N., Scroggins, B. T., and Shannon, S. D. (1995) Arthropod
Venom Citrate Inhibits Phospholipase A 2 . Toxicon 33, 763-770.
Books Edited
1. Fenton, A.W. ed. (2012) Allostery: Methods and Protocol in the
Methods in Molecular Biology series.
Poster
Presentations
1. Aron W. Fenton, Todd Holyoak, Bing Zhang, Junpeng Deng,
Charulata B. Prasannan and Qingling Tang (2013) Inhibition of human
liver pyruvate kinase by phosphorylation and cysteine oxidation share a
regulatory mechanism, removal of an activating interaction between the
rd
N-terminus and the main body of the protein. 23 Enzyme Mechanisms
Conference, Coronado, CA.
2. Aron W. Fenton, Todd Holyoak, Bing Zhang, Junpeng Deng,
Charulata B. Prasannan and Qingling Tang (2012) Inhibition of human
liver pyruvate kinase by phosphorylation and cysteine oxidation share a
regulatory mechanism, removal of an activating interaction between the
N-terminus and the main body of the protein. The Sixth Peptide
Engineering Meeting (PEM6), Atlanta, GA.
3. Aron W. Fenton, Todd Holyoak, Charulata B. Prasannan and Qingling
Tang (2012) Inhibition of human liver pyruvate kinase by phosphorylation
at Ser12 and oxidation at Cys436 share a regulatory mechanism,
removal of an activating interaction between the N-terminus and the
main body of the protein. Southeastern Enzyme Conference, Atlanta,
GA.
104
4. Prasannan, C.B. Villar, M.T., Artigues, A. and Fenton, A.W. (2012)
Hydrogen/deuterium exchange mass spectrometry identification of
allosterically relevant changes in rabbit muscle pyruvate kinase Gordon
Conference on Enzymes, Coenzymes and Metabolic Pathways,
Waterville Valley, NH.
5. Fenton, A.W. and Tang, Q. (2011) Mapping allostery in liver pyruvate
kinase using a whole protein, alanine-scan. Gordon Conference on
Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH.
6. Fenton, A.W. Urness, J., Alontaga, A.Y., Thuet, K., Barnett, J. and
Timmons, C. (2011) Identification of the allosteric response element(s) in
nd
the active site of mammalian pyruvate kinase isozymes. 22 Enzyme
Mechanisms Conference, St. Pete Beach, FL.
7. Fenton, A.W. and Tang, Q. (2009) Mapping Individual residues within
the N-terminus that are energetically coupled to allosteric and active site
functions of human liver pyruvate kinase. Keystone Symposia, Keystone,
CO.
8. Fenton, A. W., Thuet, K., Williams, R. E., and Trewhella, J. (2007)
Identifying Allosteric Specific Structural Changes in Rabbit Muscle
Pyruvate Kinase. Midwest Regional ACS Meeting, Kansas City, MO.
9. Fenton, A. W., Thuet, K., Williams, R. E., and Trewhella, J. (2007)
Identifying Allosteric Specific Structural Changes in Pyruvate Kinase.
st
21 Annual Gibolbs Conference on Biothermodynamics, Carbondale, Il.
10. Fenton, A. W. and Hutchinson, M. (2007) The Ser12 to Asp
Mutation of Human Liver Pyruvate Kinase Mimics Regulatory Effects of
st
Phosphorylation at Ser12. The 21 Symposium of the Protein Society,
Boston MA.
11. Thuet, K., Williams, R. E., Trewhella, J., and Fenton, A. W. (2007)
Non-allosteric Effector Analogs, Unique Tools for Identifying Allosteric
st
Specific Structural Changes in Pyruvate Kinase. 51 Annual Biophysical
Society Meeting, Baltimore, MD.
12. Hutchinson, M., Fan, L. and Fenton, A. W. (2006) Purification of
Human Liver Pyruvate Kinase from a Newly Constructed E. coli Strain
Deficient in Endogenous Pyruvate Kinase Activity. Midwest Enzyme
Conference, Chicago, Il.
13. Thuet, K., Williams, R. E., Trewhella, J., and Fenton, A. W. (2006)
Non-allosteric Analogs, Unique Tools for Identifying Allosteric Specific
Structural Changes in Pyruvate Kinase. Midwest Enzyme Conference,
Chicago, Il.
14. Dajkovic, Alex, Fenton, A., and Lutkenhaus, J. (2006) A model for
th
cooperative polymerization in a linear polymer. 20 Annual Gibbs
105
Conference on Biothermodynamics, Carbondale, Il.
15. Fenton, A. W., Williams, R., and Holyoak, T. (2006) Identification of
the Allosteric Amino Acid Binding Site in Mammalian Pyruvate Kinase at
th
1.6Å. 50 Annual Biophysical Society Meeting, Salt Lake City, UT.
16. Williams, R., Pendergrass, D. C., Blair, J. B., and Fenton, A. W.
(2005) Data Mining for Information on Allostery in Pyruvate Kinase
Isozymes. Kansas City Area Life Sciences Research Day, Kansas City,
KS.
17. Fenton, A. W. (2005) Differentiating the Chemical Requirements for
Ligand-Induced Allosteric Inhibition from those of Ligand Binding:
Phenylalanine Inhibition of Pyruvate Kinase in Phenylketonuria. Kansas
City Area Life Sciences Research Day, Kansas City, KS.
18. Fenton, A. W. and McDonald, G. (2005) Differentiating the Chemical
Requirements for Ligand-Induced Allosteric Inhibition from those of
th
Ligand Binding; Phenylalanine Inhibition of Pyruvate Kinase. 19
Annual Gibbs Conference on Biothermodynamics, Carbondale, Il.
19. Fenton, A. W. (2005) Differentiating the Chemical Requirements for
Ligand-Induced Allosteric Inhibition from those of Ligand Binding;
th
Phenylalanine Inhibition of Pyruvate Kinase. 49 Annual Biophysical
Society Meeting, Long Beach, CA.
Allosteric Inhibition and Activation in E. coli Phosphofructokinase use the
th
Annual
Same Binding Sites but Different Pathways. 47
Biophysical Society Meeting, San Antonio, TX.
21. Fenton, A. W. and Reinhart, G. D. (2003) Hybrid Tetramers Reveal
the Substrate Inhibition of E. coli Phosphofructokinase to be an Allosteric
th
Mechanism, NOT a Kinetic Mechanism. 18 Enzyme Mechanisms
Conference, Galveston Island, TX.
22. Fenton, A. W. and Reinhart, G. D. (2002) Binding Energy is Not
Conferred by Binding Site Residues Alone; the Binding Site of E. Coli
th
Phosphofructokinase. 46 Annual Biophysical Society Meeting, San
Francisco, CA.
23. Fenton, A. W. and Reinhart, G. D. (2001) Quantification of Isolated
Heterotropic
Interactions
Contributing
to
Allostery
of
th
Phosphofructokinase from E. coli.
6 Annual Structural Biology
Symposium, Sealy Center for Structural Biology, Galveston, TX.
24. Fenton, A. W. and Reinhart, G. D. (2001) Quantification of Isolated
Heterotropic
Interactions
Contributing
to
Allostery
in
th
Phosphofructokinase from E. coli. 45 Annual Biophysical Society
Meeting, Boston, MA.
25. Fenton, A. W. and Reinhart, G. D. (2000) Formation of a Modified
106
Tetramer of E. coli Phosphofructokinase with Only One Native Active
th
Site and One Native Allosteric Site. 14 Protein Society Symposium,
San Diego, CA.
Invited Scientific
Presentations
ACS-Kansas City chapter guest speaker to class of 2012 student
honorees
St. Marys University
Allosteric Regulation of Human Liver Pyruvate Kinase, Spring
2012
University of North Carolina-Charlotte, Department of Bioinformatics and
Genomics
Allosteric Regulation of Human Liver Pyruvate Kinase, Fall
2011
University of Missouri, Department of Chemistry and Biochemistry
Allosteric Regulation of Human Liver Pyruvate Kinase, Spring
2011
Pittsburg State University, Department of Chemistry
Regulation of human liver pyruvate kinase by N-terminal
modification, Fall 2010
Oklahoma State University, Department of Biochemistry and Molecular
Biology
Allosteric Regulation of Pyruvate Kinase, Fall 2009
Notre Dame, Department of Chemistry
The University of Minnesota Duluth, Department of Chemistry and
Biochemistry
Western Missouri State, Chemistry Department,
Kansas University, Department of Medicinal Chemistry,
Allosteric Regulation of Pyruvate Kinase, Spring 2008
Southwestern Oklahoma State University, Chemistry Department,
107
Allosteric Regulation of Pyruvate Kinase, Spring 2008
Pittsburg State University, Department of Chemistry,
Differentiating a Ligand’s Chemical Requirements for Allosteric
Interactions from Those for Protein Binding: Phenylalalnine
Inhibition of Pyruvate Kinase, Fall 2006
Missouri State University, Department of Biomedical Sciences,
Differentiating a Ligand’s Chemical Requirements for Allosteric
Interactions from Those for Protein Binding: Phenylalalnine
Inhibition of Pyruvate Kinase, Spring 2006
Wichita State University, Biology Department, “Characterizing the
Allosteric Amino Acid Effector of Pyruvate Kinase, Spring 2005
Kansas University, Chemistry Department, “Characterizing the Allosteric
Amino Acid Effector of Pyruvate Kinase, Spring 2005
University of Missouri-Kansas City, School of Biological Sciences,
Allosteric Regulation in Glycolytic Enzymes, Fall 2004
Talks at Scientific
Meetings
Biophysical Society Meeting 2013, Philadelphia, PA
Energetic coupling between an oxidizable cysteine and the
phosphorylatable N-terminus of human liver pyruvate kinase
Keystone Symposia on Allosteric Regulation 2009, Keystone, CO
Mapping Individual residues within the N-terminus that are
energetically coupled to allosteric and active site functions of
human liver pyruvate kinase
Reviewing for
Scientific
Journals
Grant Peer
Review
Meetings -
Science, Reviewer
PNAS, Reviewer
Journal of Molecular Biology, Reviewer
Journal of Biological Chemistry, Reviewer
Biochemistry, Reviewer
Biophysical Journal, Reviewer
Journal of the American Society for Mass Spectrometry, Reviewer
Biochemistry Journal, Reviewer
Archives of Biochemistry and Biophysics, Reviewer
Proteins: Structure, Function and Bioinformatics, Reviewer
NSF Outside Reviewer
2013
(scheduled
108
for
Oct.
2013)
Gibbs
Conference
in
Organized
Biothermodynamics, an invitation only meeting
2013 (scheduled for Nov. 2013) The Heartland Undergraduate
Biochemistry (HUB) Forum, Kansas City Kansas, guest lecturer Michael
Marletta
2012 The Heartland Undergraduate Biochemistry (HUB) Forum,
Kansas City Kansas, guest lecturer Heidi Hamm
2011 The Heartland Undergraduate Biochemistry (HUB) Forum,
Kansas City Kansas, guest lecturer Richard Hanson
Meetings –
assigned duties
2013 Biophysical Society Meeting, Philadelphia, PA – Session Chair
2012 Gordon Conference on Enzymes, Coenzymes and Metabolic
Pathways, Waterville Valley, NH. – Session Chair
Funding
Current:
RO1 DK78076
2/19/08-1/30/14
NIH/NIDDK
Role: P.I.
The goal of this project is to characterize the mechanism of allosteric
regulation in human liver pyruvate kinase, the regulation of which is vital
to controlling blood glucose.
Current:
NIH vis KUMC
7/1/12-6/30/13
University of Kansas Alzheimer’s Disease Center – Pilot Project
Role: P.I. of Pilot Project
The goal of this project is to determine if the cancer associated changes
in isozyme expression of pyruvate kinase and covalent modifications of
this protein that are associated with cell cycle re-entry are also present in
Alzheimer’s brain.
Past:
P20 RR16475 (PI: Hunt, J.S.)
1/1/04-6/30/04
NIH/NCRR BRIN
Role: K-BRIN recruitment package recipient
The goal of this project was to characterize what regions of Phe are
important for eliciting allosteric inhibition in pyruvate kinase from rabbit
muscle.
Continued
Education
Grant Writers’ Seminars and Workshops, LLC., 2004
COBRE Symposium on Protein Structure and Function (University of
Kansas, Lawrence, KS), 2004
109
Curriculum Vitae
HARVEY F. FISHER
Professor of Biochemistry
Director, Laboratory of
University of Kansas
and
School of Medicine
Molecular Biochemistry
Department of Veterans
Affairs Medical Center
4801 Linwood Blvd.
Kansas City, MO 64128-2295
EDUCATION:
1940-1943
Major - Chemistry, Western Reserve University, Cleveland,
Ohio
1946-1947
B.S. - Western Reserve University, Cleveland, Ohio
1947-1952
Ph.D. - University of Chicago, Chicago, Illinois
PRECEPTORS:
Professor Frank H. Westheimer
Professor Birgit Vennesland
THESIS TITLE:
The Enzymatic Transfer of Hydrogen
POST GRADUATE POSITIONS:
1952-1954
Postdoctoral Fellow, Coll. Phys. and Surg., Columbia
1954-1956
Project Associate, Department of Chemistry, University
University, New York, New York (Prof. David Rittenberg)
of Wisconsin, Madison, Wisconsin (Prof. Robert Alberty)
1956-1957
Instructor of Chemistry, Department of Chemistry,
University of Massachusetts, Amherst, Massachusetts
1957-1963
Senior Associate, Edsel B. Ford Institute for Medical
1963-1965
Associate Professor, University of Kansas Medical Center
Research, Detroit, Michigan
Kansas City, Kansas
110
1963-present
Director, Laboratory of Molecular Biochemistry, Department of
Veterans Affairs Medical Center, Kansas City, Missouri
1965-present
Professor of Biochemistry, University of Kansas Medical Center
1997-present
Adjunct Professor, School of Life Sciences, U. Missouri, Kansas
Kansas City, Kansas
City, Missouri
RESEARCH INTERESTS:
1. Mechanism of Enzymatic Catalysis
2. Structure-function Relationships in Macromolecules
3. Thermodynamics of Biological Interactions, including Steady State-System and
Cooperative Effects
AWARDS AND FELLOWSHIPS:
1951-1952
Atomic Energy Commission Predoctoral Fellow
1955
(Declined) Damon Runyon Cancer Fellow
1961
Recipient of National Science Foundation Travel Award from
American Society of Biological Chemists to present paper
before the Fifth International Biochemical Congress,
Moscow, USSR
1967
Recipient of an award from the American Society of Biological
Chemists for travel to the Seventh International Congress
of Biochemists, Tokyo, Japan
1978
Veterans Administration Career Scientist Award
GRANT SUPPORT:
1962-1986
NSF (The Energetics of Enzyme Catalyzed Reactions)
1968-1993
NIH (Mechanisms of Enzymatic Catalysis)
1964-1997
VA National Peer Reviewed Support (Structure-Function
Relationships in Bacterial Glutamate Dehydrogenase - $133,100
support this year)
1987
BRSG (Energetics of Enzyme-Ligand Interactions)
1990-1992
NSF (Support for Symposium on "The Thermodynamic Basis of
Protein Structure & Function)
1992-1995
NIH (Binding Energy Transduction in Enzymatic
111
Catalysis)
1993-1996
NSF (Mechanisms of Enzymatic Catalysis)
1996-1999
NSF (Mechanisms of Enzymatic Catalysis - $71,429 support this
1996
NSF (Supplement to Mechanisms of Enzymatic Catalysis,
year)
Research Experience for Undergraduates - $3,260 support)
1997-1999
NSF (Supplement to Mechanisms of Enzymatic Catalysis,
Research Experience for Undergraduates - $4,000 support)
MEMBERSHIP IN PROFESSIONAL SOCIETIES:
American Society of Biological Chemists
Sigma Xi
AAAS
Biophysical Society
MEMBERSHIP ON NATIONAL & INTERNATIONAL COMMITTEES AND EDITORIAL BOARDS:
Veterans Administration Liaison Representative to NIH Biochemistry Study Section
(1965-1968)
Member, Veterans Administration National Basic Science Advisory Panel (1966-1968)
Member, Committee of Isoenzymes and Enzyme Subunits Established by the
Commission of Editors of Biochemical Journals of the International Union of
Biochemistry.
Member, Veterans Administration Biochemistry-Biophysics Research Evaluation
Committee (1969-1971)
Member, Veterans Administration National Merit Review Board for Basic Sciences
(1981-1984)
National Correspondent, Public Policy Committee, American Society of Biological
Chemists, Division of Biological Chemistry, American Chemical Society
Chairman, Subcommittee on Extramural Grants for VA Research Established by the
Research and Education Council of the Veterans Administration
Member, Editorial Board, The Journal of Biological Chemistry (1973-1980)
Member, National Science Foundation Molecular Biology Advisory Panel (1974-1977)
Member, Editorial Board, Archives of Biochemistry and Biophysics (1981- )
Invited Expert, Editorial Board, Chemtracts (1992 - )
NSF Multi-User Instrument Panel (1996-1997)
Ad Hoc Member, FASEB Public Affairs Committee (1997)
112
SYMPOSIA PARTICIPATION:
Symposium on the Mechanism of Action of the Pyridine Nucleotide-Dependent
Dehydrogenases in Honor of Hugo Theorell, University of Kentucky, (Invited
Speaker), March 16-19, 1965.
Seventh International Congress of Biochemistry, Tokyo, Japan (Invited Speaker),
August 19-25, 1967.
Symposium on Molecular Conformation of Proteins in Solution at the meeting of the
Division of Biological Chemistry of the American Chemical Society, Atlantic City,
New Jersey, (Chairman, Sept. 8-13, 1968).
Second Conference on Enzyme Mechanisms, Santa Barbara, California, (Invited
Speaker), December 16-29, 1970.
Intra-Science Symposium on the mechanisms of Enzyme Action, Santa Monica,
California (Invited Speaker), December 2-3, 1971.
Conference on Techniques of Microcalorimetric Investigations of Cellular Systems
with Special Reference to the Clinical Field, Lund, Sweden, (Session Chairman),
July 9-11, 1973.
International Symposium on New Applications of Microcalorimetry as Applied in
Biomedical Research, Bethesda, Maryland, (Invited Speaker), January 28-29, 1976.
Frank H. Westheimer Symposium on Enzymes, Cincinnati, Ohio, (Invited Speaker),
October 22, 1976.
Biochemistry Present and Future, Twelfth Midwest American Chemical Society
Meeting, Kansas City, Missouri, (Chairman), October 28-29, 1976.
International Symposium on Mechanisms of Oxidizing Enzymes, La Paz, Baja,
California Sur, Mexico, (Invited Speaker), December 5-7, 1977.
Symposium on Electron Transfer and Interfacial Behavior of Molecules by
Biological Importance, Electrochemical Society Meeting, St. Louis, Missouri
(Invited Speaker), May 13-14, 1980.
113
IUPAC Conference on Chemical Thermodynamics/The 39th Annual Calorimetry
Conference, Hamilton, Canada, (Invited Speaker), August 13-17, 1984.
Watkins Midwest Biophysics and Biophysical Chemistry Conference, Wichita,
Kansas (Invited Speaker), October 3-4, 1986.
International Symposium on Thermodynamics Applied to Biological Systems,
Santa Margherita Ligure, Italy, September 11-17, 1988.
Molecular Mechanisms of Protein Recognition, Kyoto, Japan (Invited Speaker),
March 12-16, 1989.
International Symposium in Honor of Rufus Lumry, "The Thermodynamic Basis of Protein
Structure and Function", Kansas City, Missouri (Local Chairman),
October 4-6, 1990.
Workshop on Transmembrane Electric Fields, Elkridge, Maryland (Invited Speaker)
October 23-26, 1990.
National Meeting of the Biophysical Society, February 1998, Kansas City (Local
Chairman)
Co-Chairman and speaker, International Symposium on Mechanisms of Enzymatic Catalysis,
Guilin, China (May 1998)
UNDERGRADUATE SCIENCE MENTORING
Science Pioneers (1991 - )
Ryan McCue, Summer Research Project (1990)
Peter Meyerdirk, “Unique Academic Project” and visit to lab (1995)
Chad Brocker, Shadow-ship Program (1995)
UMKC High School Science, Mathematics and Technology Institute, Phase II, tour of
laboratory (1997)
Robert Pierce, David Weiss, Lawrence Indyk, David Kristensen NSF Research
Experience for Undergraduates (1996-1998)
MAJOR PUBLICATIONS
114
1.
F. H. Westheimer, H.F. Fisher, E.E. Conn and B. Vennesland. The Enzymatic Transfer of
Hydrogen from Alcohol to DPN. J. Am. Chem. Soc., 73:2403, 1951.
2.
H.F. Fisher, E.E. Conn, B. Vennesland and F.H. Westheimer. The Enzymatic Transfer of
Hydrogen. I. The Reaction Catalyzed by Alcohol Dehydrogenase. J. Biol. Chem., 202:687,
1953.
3.
F.A. Loewus, P. Ofner, H.F. Fisher, F.H. Westheimer and B. Vennesland. The Enzymatic
Transfer to Hydrogen. II. The Reaction Catalyzed by Lactic Dehydrogenase. J. Biol. Chem.,
202:699, 1953.
4.
H.F. Fisher, A.I. Krasna and D. Rittenberg. The Interaction of Hydrogenase with Oxygen. J.
Biol. Chem., 209:569, 1954.
5.
H.F. Fisher, C. Frieden, J.S. McKinley McKee and R.A. Alberty. Concerning the
Stereospecificity of the Fumarase Reaction and the Demonstration of a New intermediate. J. Am.
Chem. Soc., 77:4436, 1955.
6.
R.A. Alberty, W.G. Miller and H.F. Fisher. Studies on the Enzyme Fumarase. VI. The
Incorporation of Deuterium into L-Malate During the Reaction in Deuterium Oxide. J. Am. Chem.
Soc., 79:3973, 1957.
7.
H.F. Fisher and L.L. McGregor. On the Nature of the Fluorescence of Enzyme-DPNH
Complexes. Biochim. Biophys. Acta, 38:562, 1960.
8.
H.F. Fisher. The Mechanism of the Glutamic Dehydrogenase Reaction. I. The Molecularity of
the First Complex Formed. J. Biol. Chem., 235:1830, 1960.
9.
H.F. Fisher and L.L. McGregor. The Nature of the Fluorescence of an Enzyme-Reduced
Coenzyme-Reduced Substrate Complex. Biochim. Biophys Acta, 43:557, 1960.
10.
H.F. Fisher and L.L. McGregor. The Role of the Ammonium Moiety in the Glutamic
Dehydrogenase Reaction. Biochem. Biophys. Res. Commun., 3:629, 1960.
11.
H.F. Fisher and L.L. McGregor. The Mechanism of the Glutamic Dehydrogenase Reaction. II.
Substrate Specificity of the Enzyme. J. Biol. Chem., 236:791, 1961.
12.
H.F. Fisher. The Appearance of Substrate Binding Terms in the V max Expression of the Enzyme
Reactions. J. Theoret. Biol., 3:484, 1962.
13.
H.F. Fisher, L.L. McGregor and U. Power. The Nature of the Alkaline Dissociation of the
Glutamic Dehydrogenase Molecule. Biochem. Biophys. Res. Commun., 8:402, 1962.
115
14.
H.F. Fisher, L.L. McGregor land D.G. Cross. The Role of Tyrosyl-Hydrogen Bonds in the
Quaternary Structure of the Glutamic Dehydrogenase Molecule. Biochim. Biophys. Acta, 65:175,
1962.
15.
H.F. Fisher, D.G. Cross and L.L. McGregor. Catalytic Activity of Subunits of Glutamic
Dehydrogenase. Nature, 196:895, 1962.
16.
H.F. Fisher. A Nomenclature for the Quaternary Structure of an Enzyme Molecule. Nature,
198:665, 1963.
17.
H.F. Fisher. A Limiting Law Relating the Size and Shape of Protein Molecules to Their
Composition. Proc. Nat. Acad. Sci., 51:1285-1291, 1964.
18.
H.F. Fisher and D.G. Cross. Spectrophotometric Studies of the Quaternary Structure of Proteins.
I. The Method of Concentration-Difference Spectra. Archives of Biochem. and Biophys.,
110:217-221, 1965.
19.
D.G. Cross and H.F. Fisher. Spectrophotometric Studies of the Quaternary Structure of Proteins.
II. The
f,
Glutamic
 Split Dehydrogenase.
o
Archives of Biochem. and Biophys., 110:222-226,
1965.
20.
H.F. Fisher, D.G. Cross and L.L. McGregor. The Independence of the Substrate Specificity of
Glutamic Dehydrogenase on its State of Aggregation. Biochim. Biophys. Acta, 99:165-167, 1965.
21.
H.F. Fisher and D.G. Cross. The Involvement of Tryptophan Residue of Glutamate
Dehydrogenase in the Binding of L-Glutamate. Biochem. Biophys. Res. Commun., 20:120-123,
1965.
22.
H.F. Fisher, An Upper Limit to the Amount of Hydration of a Protein Molecule. A Corollary to
the "Limiting Law of Protein Structure". Biochim. Biophys. Acta, 109:544-550, 1965.
23.
W.C. McCabe and H.F. Fisher. Measurement of the Excluded Volume of Protein Molecules by
Differential Spectroscopy in the Near Intra-Red. Nature, 207:1274-1276, 1965.
24.
D.G. Cross and H.F. Fisher. The Spatial Location of Chromophoric Residues in L-Glutamate
Dehydrogenase. Biochemistry, 5:880-885, 1966.
25.
H.F. Fisher and D.G. Cross. Enzyme-Coenzyme Complexes of Pyridine Nucleotide-Linked
Dehydrogenases. Science, 153:414-415, 1966.
26.
H.F. Fisher. Geometrical Considerations for the Theory of Protein Structure - Symposium on
Higher Order Structures in Biopolymers - Seventh International Congress of Biochemistry, Tokyo,
1967.
116
27.
H.F. Fisher and L.L. McGregor. The Ability of Reduced Nicotinamide Mononucleotide to
Function as a Hydrogen Donor in the Glutamic Dehydrogenase Reaction. Biochem. Biophys. Res.
Commun., 34:627-632, 1969.
28.
D.G. Cross, and H.F. Fisher. Conformation and Conformational Changes of Reduced
Diphosphospyridine Nucleotide in Solution. Biochemistry, 8:1147-1155, 1969.
29.
H.F. Fisher and J.R. Bard. The Kinetic Measurement of the 
- Dissociation of Glutamate
Dehydrogenase. Biochim. Biophys. Acta, 188:168-170, 1969.
30.
H.F. Fisher, D.L. Adija and D.G. Cross. Dehydrogenase-Reduced Coenzyme Difference Spectra,
Their Resolution and Relationship to the Stereospecificity of Hydrogen Transfer. Biochemistry,
8:4424-4431, 1969.
31.
H.F. Fisher and J.R. Bard. An Intermediate in the pH-Induced Dissociation of Glutamate
Dehydrogenase. Biochem. Biophys. Res. Commun., 37:581-586, 1969.
32.
H.F. Fisher. "The Structure of the Glutamic Dehydrogenase Molecule and Its Subunits." in The
Mechanism of Action of Dehydrogenases, eds., G.W.Schwert and A.D. Winer. The University
Press of Kentucky, 223-253, 1970.
33.
W.C. McCabe and H.F. Fisher. A Near-Infrared Spectroscopic Method for Investigating the
Hydration of a Solute in Aqueous Solution. J. Phys. Chem., 74:2990-2998, 1970.
34.
D.G. Cross and H.F. Fisher. The Mechanism of Glutamate Dehydrogenase Reaction. III. The
Binding of Ligands at Multiple Subsites and Resulting Kinetics Effects. J. Biol. Chem., 245:26122621, 1970.
35.
W.C. McCabe, S. Subramanian and H.F. Fisher. A Near-Infrared Spectroscopic Investigation of
the Effect of Temperature on the Structure of Water. J. Phys. Chem., 74:4360-4369, 1970.
36.
H.F. Fisher, R.E. Gates and D.G. Cross. A Ligand Exclusion Theory of Allosteric Effects.
Nature, 228:247-249, 1970.
37.
H.F. Fisher, J.R. Bard and R.A. Prough. Transient-State Intermediates Involved in the Hydride
Transfer Step of the Glutamate Dehydrogenase Reaction. Biochem. Biophys. Res. Commun.,
41:601-607, 1970.
38.
S. Subramanian and H.F. Fisher. Splitting of Water Absorption Band by Perchlorate Anion Assignment of High Frequency Band. Roczniki Chemii, 45:933-935, 1971.
39.
A.M. Fiskin, E.F.J. van Bruggen and H.F. Fisher. Structure and Function of Oligomeric
Dehydrogenases. Electron Microscopic Studies of Beef Liver Glutamate Dehydrogenase
Quaternary Structure. Biochemistry, 10:2396-2408, 1971.
117
40.
R.E. Gates and H.F. Fisher. The Restrictions of Sequence on the Thickness of Globular Protein
Molecules. Proc. Nat. Acad. Sci., 68:2928-2931, 1971.
41.
S. Subramanian and H.F. Fisher. Detection of Polar Side Chain Hydration in Polypeptides by
Near-Infrared Spectroscopy. Biopolymers, II:1305-1309, 1972.
42.
S. Subramanian and H.F. Fisher. Near-Infrared Spectral Studies on the Effects of Perchlorate and
Tetrafluoroborate Ions on Water Structure. J. Phys. Chem. 76:84-89, 1972.
43.
H.F.Fisher, J.M. Culver and R.A. Prough. The Independence of Adenosine-5 
-Diphosphate
Binding and the State of Association of L-Glutamate Dehydrogenase. Biochem. Biophys. Res.
Commun., 461:462-1468, 1972.
44.
R. A. Prough, J.M. Culver and H.F. Fisher. Spectrophotometric Evidence for a Glutamate
Dehydrogenase--L-Leucine Complex. Arch. Biochem. Biophys., 149:414-418, 1972.
45.
R.A. Prough and H.F. Fisher. Specificity and Spectral Resolution of an L-Glutamate
Dehydrogenase-Nonocarboxylic Amino Acid Complex. Biochemistry, 11:2479-2482, 1972.
46.
A.H. Colen, R.A. Prough and H.F. Fisher. The Mechanism of Glutamate Dehydrogenase
Reaction. IV. Evidence for Random and Rapid Binding of Substrate and Coenzyme in the Burst
Phase. J. Biol. Chem., 247:7905-7909, 1972.
47.
D.G. Cross, L.L. McGregor and H.F. Fisher. The Binding of 
-ketoglutarate in a Binary
Complex and in a Ternary Complex with NADP+ by L-Glutamate Dehydrogenase. Biochim.
Biophys. Acta, 289:28-36, 1972.
48.
H.F. Fisher. "Glutamate Dehydrogenase--Ligand Complexes and Their Relationship to the
Mechanism of the Reaction," in Advances in Enzymology, ed., A. Meister, Interscience Publishers,
New York, 369-417, 1973.
49.
H.F.Fisher and D.G. Cross. "Ligand Exclusion As An Alternate Theory of Allosteric Effects," in
Intra-Science Chemistry Reports, ed., N. Kharasch, Intra-Science Research Foundation, Vol. 6,
pp. 61-71, 1972.
50.
R.A. Prough, A.H. Colen and H.F. Fisher. Spectrophotometric Observation of a Glutamate
Dehydrogenase-L-Glutamate Complex. Biochim. Biophys. Acta, 284:16-19, 1972.
51.
A. Brown, J.M. Culver and H.F.Fisher. Mechanism of Inactivation of L-Glutamate
Dehydrogenase by Pyridoxal and Pyridoxal Phosphate. Biochemistry, 12:4367-4373, 1973.
118
52.
R.A. Prough, J.M. Culver and H.F. Fisher. The Mechanism of Activation of Glutamate
Dehydrogenase-Catalyzed Reactions by Two Different Cooperatively Bound Activators. J.
Biol.Chem., 248:8528-8533, 1973.
53.
A.H. Colen, D.G. Cross and H.F.Fisher. Two-Step Binding of Adenosine Diphosphate to LGlutamate Dehydrogenase. Effect on Binding of Reduced Nicotinamide Adenine Diphosphate
and on Enzymatic Catalysis. Biochemistry, 13:2341-2347, 1974.
54.
A.H. Colen, R.R. Wilkinson and H.F.Fisher. Location of Deuterium Oxide Solvent Isotope
Effects in the Glutamate Dehydrogenase Reaction. J. Biol. Chem., 250:5243-5246, 1975.
55.
D.G. Cross, A. Brown, and H.F. Fisher. Hydrogen-Deuterium Exchange in Nucleosides and
Nucleotides. A Mechanism for Exchange of the Exocyclic Amino Hydrogens of Adenosine.
Biochemistry, 14:2745-2749, 1975.
56.
S. Subramanian, D.C. Stickel and H.F. Fisher. Thermodynamics of Complex Formation Between
Bovine Liver Glutamate Dehydrogenase and Analogs of ADP. J. Biol. Chem., 250:5885-5889,
1975
57.
D.G. Cross, A. Brown and H.F. Fisher. Hydrogen Exchange at the Amide Group of Reduced
Pyridine Nucleotides and the Inhibition of that Reaction by Dehydrogenases. J. Biol. Chem.,
251:1785-1788, 1976.
58.
A. Brown and H.F. Fisher. A Comparison of the Glutamate Dehydrogenase Catalyzed Oxidation
of NADPH by Trinitrobenzene Sulfonate with the Uncatalyzed Reaction. J. Amer. Chem. Soc.,
98:5682-5688, 1976.
59.
A.H. Colen, R.R. Wilkinson and H.F. Fisher. The Transient-State Kinetics of L-Glutamate
Dehydrogenase: pH Dependence of the Burst Rate Parameters. Biochim. Biophys. Acta, 481:377383, 1977.
60.
H.F. Fisher, D.C.Stickel, A. Brown and D. Cerretti. Determination of the Thermodynamic
Parameters of Individual Steps of the Pyruvate-Oxime Formation by Rapid, Continuous Flow
Microcalorimetry, J. Amer. Chem.Soc., 99:8180-8182, 1977.
61.
A. Brown, A.H. Colen and H.F. Fisher. Effect of Ammonia on the Glutamate Dehydrogenase
Catalyzed Oxidative Deamination of L-Glutamate: I. Production of an Ammonia-Containing
Intermediate in the "Burst" Phase. Biochemistry, 17:2031-2034, 1978.
62.
H.F. Fisher and A.H. Colen. "The Mechanism and Energetics of the Glutamate Dehydrogenase
Reaction," in Developments in Biochemistry: II. Mechanisms of Oxidizing Enzymes. eds., T.P.
Singer and R.N. Ondarza, Elsevier North Holland, Inc., 95-108, 1978.
63.
S. Subramanian, D. Stickel, A.H. Colen and Harvey F. Fisher. Thermodynamics of Heterotropic
Interactions. The Glutamate Dehydrogenase-NADPH-Glutamate Complex. J. Biol. Chem.,
253:8369-8374, 1978.
119
64.
B.A. Bradley, A.H. Colen and H.F. Fisher. The Effects of Methanol on the Glutamate
Dehydrogenase Reaction at 0
Biophys. J., 25:555-561, 1979.
C.
65.
A. Brown, A.H. Colen and H.F. Fisher. Effect of Ammonia on the Glutamate Dehydrogenase
Catalyzed Oxidative Deamination of L-Glutamate. II. The Steady State. Biochemistry, 18:59245928, 1979.
66.
H.F. Fisher, S. Subramanian, D. Stickel, and A.H. Colen. The Thermodynamics of a Negatively
Interacting Allosteric Effector System: The Glutamate Dehydrogenase-NADPH-ADP Complexes.
J. Biol. Chem., 255:2509-2513, 1980.
67.
H.F. Fisher and D.C. Stickel. Thermodynamic Interaction Parameters of a Reactive Enzyme
Ternary Complex: Glutamate Dehydrogenase--NADPH--Ketoglutarate. FEBS Letters, 113:1114, 1980.
68.
H.F. Fisher, D.C. Stickel and A.H.Colen. pH Dependent Thermodynamic Parameters of the
Glutamate Dehydrogenase--Ketoglutarate-NADPH Complex. Biochim. Biophys. Acta, 615:2733, 1980.
69.
R.E. Johnson, P.J. Andree and H.F. Fisher. Cryoenzymological Studies of the Oxidative
Deamination of L-Glutamate by Glutamate Dehydrogenase: Spectral Resolution of Transient and
Product Complexes. J. Biol. Chem., 256:3817-3821, 1981.
70.
R.E. Johnson, P.J. Andree and H.F. Fisher. Evidence for a Change in the Structure of Glutamate
Dehydrogenase During its Catalytic Cycle Under Cryogenic Conditions. J. Biol. Chem.,
256:6381-6384, 1981.
71.
A.H. Colen, R.T. Medary and H.F. Fisher. Transient Phase Energetics of the Oxidative
Deamination of L-Glutamate by L-Glutamate Dehydrogenase and NADP: A Reaction with a
Large Negative Heat Capacity of Activation. Biopolymers, 20:879-889, 1981.
72.
H.F. Fisher, A.H. Colen and R.T. Medary. Temperature-Dependent
in Equilibrium Between Macrostates of an Enzyme. Nature, 292:271-272, 1981.
73.
R. Srinivasan, R.T. Medary, H.F. Fisher, D.J. Norris and R. Stewart. The PyridiniumDihydropyridine System. Reduction Potentials and the Mechanism of Oxidation of 1,4Dihydropyridines by a Schiff Base. J. Am.Chem. Soc., 104:807-812, 1982.
74.
T.S. Viswanathan, R.E. Johnson and H.F. Fisher. -Ketoglutaric Acid-Solution Structure and the
Active Form for Reductive Amination by Bovine Liver Glutamate Dehydrogenase. Biochemistry,
21:339-345, 1982.
120
Cp
by
a Shift
Generated
75.
T.S. Viswanathan, C.E. Hignite and H.F. Fisher. Determination of Carbonyl Oxygen Exchange
Rates in 
-Ketoacids by Gas Chromatography-Mass Spectrometry. Analytical Biochemistry,
123:295-302, 1982.
76.
H.F. Fisher, R. Srinivasan and A.E. Rougvie. Glutamate Dehydrogenase Catalyzes the Reduction
of A Schiff Base,
-Pyrroline-2-Carboxylic Acid, by Reduced Pyridine Dinucleotide. J. Biol.

Chem., 257:13208-13210, 1982.
77.
A.H. Colen, R.E. Johnson and H.F. Fisher. The Effect of Cryosolvent on the Transient Kinetics of
the Glutamate Dehydrogenase Reaction. Biochemistry, 21:6695-6699, 1982.
78.
R. Srinivasan and H.F. Fisher. Configurational, Conformational and Solvent Effects on the
Reduction of a Schiff Base by Reduced Pyridine Nucleotide Analogs. Arch. Biochem. Biophys.,
223:453-457, 1983.
79.
H.F. Fisher. "L-Glutamate Dehydrogenase from Bovine Liver" in Methods in Enzymology, ed., A.
Meister, Academic Press, New York, 16-27, 1985.
80.
H.F. Fisher, R.T. Medary, E.J. Wykes and C.S. Wolfe. Thermodynamic Interactions in the
Glutamate Dehydrogenase-NADPH-Oxalylglycine Complex. J. Biol. Chem., 259:4105-4110,
1984.
81.
T.S. Viswanathan and H.F. Fisher. Carbonyl Oxygen Exchange Evidence of Imine Formation in
Glutamate Dehydrogenase Reaction and Identification of the "Occult Role" of NADPH. Proc.
Natl. Acad. Sci., 81:2747-2751, 1984.
82.
R. Srinivasan and H.F. Fisher. The Reversible Reduction of an 
-imino Acid to 
-amino Acid
Catalyzed by Glutamate Dehydrogenase: The Effect of Ionizable Function Groups. Biochemistry,
24:618-622, 1985.
83.
R. Srinivasan and H.F. Fisher. A Comparison of the Energetics of the Spontaneous and the
Glutamate Dehydrogenase Catalyzed 
-Imino Acid---Amino Acid Interconversion.
Biochemistry, 24:5356-5360, 1985.
84.
R. Srinivasan and H.F. Fisher. Deuterium Isotope Effects for the Nonenzymatic and Glutamate
Dehydrogenase-Catalyzed Reduction of an 
-imino Acid by NADH. J. Am. Chem. Soc.,
107:4801-4805, 1985.
85.
H.F. Fisher, S. Maniscalco, C. Wolfe, land R. Srinivasan. NADPH-Binding-Induced Proton
Ionization as a Cause of Non-Linear Heat Capacity Changes in Glutamate Dehydrogenase.
Biochemistry, 25:2910-2915, 1986.
86.
P. Chalabi, S. Maniscalco, L.E. Cohn, and H.F. Fisher. The Effects of an Acetate Sensitive Anion
Binding Site on NADPH Binding in Glutamate Dehydrogenase. Biochim. Biophys. Acta,
913:103-110, 1987.
121
87.
R. Srinivasan and H.F. Fisher. Structural Features Facilitating the Glutamate Dehydrogenase
Catalyzed -imino Acid 
-amino Acid Interconversion. Arch. Biochem. Biophys., 246:743-750,
1986.
88.
H.F. Fisher. "A Unifying Model of the Thermodynamics of Formation of Dehydrogenase-ligand
Complexes" in Advances in Enzymology, Vol. 61, ed. A. Meister, John Wiley and Sons, 1-46,
1988.
89.
H.F.Fisher, S. Pazhanisamy, R.T. Medary. The Anomalous Properties of the Glutamate
Dehydrogenase--NADPH---Ketoglutarate Complex are not Ascribable to Carbonyl Addition
Reactions. J. Biol. Chem., 262:11684-11687, 1987.
90.
R. Srinivasan, T.S. Viswanathan, and H.F. Fisher. Mechanism of Formation of Bound 
Iminoglutarate from 
-Ketoglutarate in the Glutamate Dehydrogenase Reaction. J.Biol. Chem.,
263:2304-2308, 1988.
91.
H.F. Fisher, S. Maniscalco, N. Singh, S.A. Adediran. Proton/Product Time Course Ratios: A
New Approach to Transient-State Kinetic Analysis. J. Biol. Chem., 263:11704-11710, 1988.
92.
H.F. Fisher and N. Singh. The Meaning of Interaction Parameters in Two-State Protein
Complexes. J. Biol. Phys., 17:213-220, 1990.
93.
H.F. Fisher and N. Singh. Transduction of Enzyme-Ligand Binding Energy into Catalytic Driving
Force. FEBS Letters, 294:1-5, 1991.
94.
H.F. Fisher, S. Maniscalco, N. Singh, R.N. Mehrotra, and R. Srinivasan. A Slow Obligatory
Proton Release Step Precedes Hydride Transfer in the Liver Glutamate Dehydrogenase Catalytic
Mechanism. Biochim. Biophys. Acta, 1119:52-56, 1992.
95.
N. Singh, H.F. Fisher, and R.N. Mehrotra. Resolution of Rate Constants in a two-Step Binding
Process with a Single Observable Relaxation. J. Phys. Chem., 96:5688-5689, 1992.
96.
H.F. Fisher. (J.M. Sanchez-Ruiz, Theoretical Analysis of Lumry-Eyring Models in Differential
Scanning Calorimetry, Biophys. J., 61:921, 1992) Reviewed in Chemtracts 3:313-318 (1992).
97.
N. Singh, S. Maniscalco, and H.F. Fisher. The Real-Time Resolution of Proton-Related
Transient-State Steps in an Enzymatic Reaction. J. Biol. Chem., 268:21-28 (1993)
98.
H.F. Fisher and N. Singh. "Calorimetric Methods for Interpreting Protein-Ligand Interactions." in
Methods in Enzymology, Vol. 259, eds. G.K. Ackers and M.L. Johnson, Academic Press, Inc.,
194-221, 1995.
122
99.
N. Singh and H.F. Fisher. Protein-ligand Interactions as a Driving Force for a High-enthalpy
Two-state Transition in Glutamate Dehydrogenase: The Opposing Roles of Phosphate and
Acetate Ions. Arch. Biochem. Biophys, 312:38-44 (1994).
100.
S. Pazhanisamy, S.J. Maniscalco, N. Singh, and H.F. Fisher. A Kinetic Mechanism of the
Allosteric Control of Enzyme-coenzyme Binding: Glutamate Dehydrogenase-NADPHPhosphate-Acetate-Hydrogen Ion Interactions. Biochemistry , 33:10381-10385 (1994).
101.
S.K. Saha, S.J. Maniscalco, N. Singh, H.F. Fisher. The Demonstration of a Glutamate
Dehydrognease--NADP-L-Glutamate Charge-transfer Complex and Its Location on the Reaction
Pathway. J. Biol. Chem. 269:29592-29597 (1994).
102.
5
-3H.F. Fisher. (L. Xue, P. Talalay, and A.S. Mildvan, Studies of the Mechanism of the 
Ketosteroid Isomerase Reaction by Substrate, Solvent, and Combined Kinetic Deuterium Isotope
Effects on Wild-Type and Mutant Enzymes, Biochemistry, 29:7491, 1990; L Xue, A. Kuliopulos,
5
-3A.S. Mildvan, and P. Talalay, Catalytic Mechanism of an Active-Site Mutant (D38N) of 
Ketosteroid Isomerase. Direct Spectroscopic Evidence for Dienol Intermediates, Biochemistry,
30:4991, 1991; and L.Xue, P. Talalay, and A.S. Mildvan, Studies of the Catalytic Mechanism of
5
-3-Ketosteroid Isomerase by Kinetic Deuterium Isotope
an Active-Site Mutant (Y14F) of 
Effects, Biochemistry, 30:10858, 1991) Critical Reviews in Chemtracts, 6, 69-75 (1996).
103.
H.F. Fisher and S.K. Saha. The Interpretation of Transient-State Kinetic Isotope Effects.
Biochemistry, 35, 83-88 (1996).
104.
S.J. Maniscalco, S.K. Saha, P. Vicedomine, and H.F. Fisher. A Difference in the Sequence of
Steps in the Reactions Catalyzed by Two Closely Homologous Forms of Glutamate
Dehydrogenase. Biochemistry, 35, 89-94 (1996).
105.
N. Singh, Z. Liu, and H.F. Fisher. The Existence of a Hexameric Intermediate with
Molten-Globule-Like Properties in the Thermal Denaturation of Bovine-Liver Glutamate
Dehydrogenase. Biophysical Chemistry, 63, 27-36 (1996) .
106.
S.K. Saha, S.J. Maniscalco, and H.F. Fisher. Mechanistic Interpretation of Tryptophan
Fluorescence Quenching in the Time Courses of Glutamate Dehydrogenase Catalyzed Reaction.
Biochemistry, 35, 16483-16488 (1996).
107.
H.F. Fisher and J. Tally. Isoergonic Cooperativity in Glutamate Dehydrogenase
Complexes: A New Form of Allostery. Biochemistry, 36 #36, 10807-10810 (1997).
108.
S.K. Saha, S.J. Maniscalco, and H.F. Fisher. The Use of Multiwavelength Kinetic Analysis
Approach to Identify and Characterize Intermediate Complexes in the Reductive Amination
Reaction Catalyzed by Bovine Liver Glutamate Dehydrogenase. Biochim. Biophys. Acta, 1382, 812 (1998).
109.
H.F. Fisher and J. Tally. Isoergonic Cooperativity—A Novel Form of Allostery. Methods in
Enzymology, 295, 331-349, (1998).
123
110.
L. Indyk and H.F. Fisher. Theoretical Aspects of Isothermal Titration Calorimetry. Methods in
Enzymology, 295, 350-364 (1998).
111. H.F. Fisher, S.K. Saha, and S.J. Maniscalco, Identification and Characterization of Kinetically
Competent Carbinolamine and α-Iminoglutarate Complexes in the Glutamate DehydrogenaseCatalyzed Oxidation of L-Glutamate Using a Multiwavelength Transient State Approach,
Biochemistry, 37, 14585-14590 (1998).
112. H.F. Fisher, Transient State Kinetic Approaches to the Resolution of Enzyme Mechanisms, in
Enzymatic Mechanisms, eds. P.A. Frey and D.B. Northup IOS Press 264-277 (1998).
113. S.K. Saha, and H.F. Fisher, The Location of Active Site Opening and Closing Events in the
Prehydride Transfer Phase of the Oxidative Deamination Reaction Catalyzed by Bovine Liver
Glutamate Dehydrogenase Using a Novel pH Jump Approach. BBA 1431, 261-265 (1999).
114. H.F. Fisher, Protein Ligand Interactions Molecular Basis, Encyclopedia of Life Sciences, (In Press,
2000).
115. H.F. Fisher, Glutamate Dehydrogenase as a Molecular Machine, Advances in Enzymology, (In Press,
2000).
116. H.F. Fisher, Transient State Enzyme Kinetics (Accounts of Chemical Research,
Invited Paper, In Press)
124
125
CURRICULUM VITAE
Mark Thomas Fisher
HOME ADDRESS:
WORK ADDRESS:
4300 West 71st Terrace
Prairie Village, KS 66208
Biology
Department of Biochemistry and Molecular
(913) 432-1668
Kansas City, KS 66160-7421
Tel: (913) 588-6940
Fax: (913) 588-7440
DATE AND PLACE OF BIRTH: June 21, 1954; Spokane, WA
Research interests:
Roles of molecular chaperones in protein-folding and
oligomer assembly, Thermodynamics and kinetics of protein
folding, second derivative UV spectroscopy, electron transfer
in proteins
EDUCATION: 1976 - B.S.
Microbiology, Purdue University, Lafayette, IN
1982 - B.S.
Chemistry, Purdue University, Hammond, IN
1987 - Ph.D.Biochemistry, University of Illinois, Urbana, IL
PROFESSIONAL EXPERIENCE:
2008-present
Professor, Department of Biochemistry and Molecular
Biology, University of Kansas Medical Center, Kansas
City, KS
1997- 2008
Associate Professor, Department of Biochemistry and
Molecular Biology, University of Kansas Medical Center,
Kansas City, KS
1995-Present
Adjunct Member of University of Missouri Doctoral
Faculty
1992 - 1997
Assistant Professor, Department of Biochemistry and
Molecular Biology, University of Kansas Medical Center,
126
Kansas City, KS
1987 - 1992
Staff Fellow, Laboratory of Biochemistry, National Heart,
Lung, and Blood Institute, National Institutes of Health,
Bethesda, MD
Jan-May 1987
Research Associate, Department of Biochemistry,
University of Illinois
1983 - 1987
Research Assistant, Department of Biochemistry,
1982 - 1983
Teaching Assistant, Department of Biochemistry,
1981 - 1982
Undergraduate Research Assistant, Department of
Chemistry, Purdue University
1981 - 1982
Laboratory Technician III, Department of Biochemistry,
Rush-St. Luke's Presbyterian Hospital, Chicago, IL
Grant Study Sections
Member NIH Special Study Section SPECIAL EMPHASIS PANEL
ZRG1 BST-L (50) Targeting Disease Caused by Protein Misfolding or
Misprocessing
February 13-14, 2006
Member NSF Study Section SBIR/STTR Bioprocess I. Division of Industrial
Innovation and Partnerships (IIP) January 31, 2007
Member NIH Special Study Section SPECIAL EMPHASIS PANEL
ZRG1 BST-Q(52) Targeting Disease Caused by Protein Misfolding or
Misprocessing
June 8, 2007.
Member NSF Study Section SBIR/STTR Bioprocess, Phase II. Division of
Industrial Innovation and Partnerships (IIP) November 1, 2007, October 21,
2008.
PATENTS
Fisher Mark .T. and Voziyan Paul A. “Chaperonin and osmolyte protein folding
and related screening methods” Patent # 6,887,682 United States Patent and
Trademark Office issued December 2004 – Published May 6 2005.
127
Submitted a continuation in Part (CIP) (additional claims to commercialized array
– will be issued as another patent) Mark T. Fisher and Paul A Voziyan
“Chaperonin and osmolyte protein folding and related screening methods”
Attorney Docket No. 506275-0025. In process.
Mark T. Fisher and Hiroo Katayama UNITED STATES Pending PATENT :
OSMOLYTE MIXTURE FOR PROTEIN STABILIZATION, Attorney Docket
No.: 506275-0044
Mark T. Fisher and Subhashchandra Naik UNITED STATES Pending PATENT:
SYSTEMS AND METHODS FOR IDENTIFYING PROTEIN STABILIZERS
Attorney Docket No
Current Funding:
D. Research Support (Current)
•
NIH T32A1070089 NIH Training Grant- Biotechnology Grant, (Mentor Graduate student Training Fellowship)
•
NIH R01- CryoEM analysis of Anthrax Toxin Pore Complexes , March 1,
2011- Feb 29th 2014. 775,000 direct and indirect
•
Leid Grant – Detection/ Prevention of Preaggregate Formation in
Concentrated Therapeutics February 15, 2013-Feb 14, 2014. 30 K
Direct costs D. Volkin coPI.
•
KUCTC Proof of Concept grant “Designing Broad-Based HTS Platforms to
Detect and Stabilize Partially Folded/Misfolded Protein Products.” April 1,
2013-March 31, 2014. 50 K D. Volkin coPI.
Completed (last 4 years)
Institute for Advancing Medical Innovation. - “Lead Compound Identification of
Direct Chemical Correctors of the Misfolding of Cystic Fibrosis Transmembrane
Regulator using a Novel Chaperonin Screening System” $100,000 K Total. June
1, 2010- December 31, 2011.
Oncimmune Industrial Refolding Contract – Successful Cancer Detection Product
Development. Refolded 11 Oncogenic proteins. Sept 2008-Sept 2010
MCB 0445936 Fisher (PI)
Foundation (5 publications)
07/15/05-07/14/09 National Science
128
“Structural and Energetic Effects of Protein Substrate Binding on GroEL
Chaperonin”
Kansas City Life Sciences initiative – Hookworm Antigen development and
Production July 2009-June 2010. $45,000
Courses taught:
Biochem 801 Medical Biochemistry discussion groups
IGPBS 891 Module 1
Biochem 923 Protein Structure and function
Biochem 802 – Formal Seminar Course
Biochem 805 - independent study biochemistry course
Biochem 808 – Physical Biochemistry… renamed Methods for Analyzing
Biomolecules
Biochem 862 – Literature series – all Biochemistry Graduate students
IGPBS Introductory lecture series
GSMC 859- Research Rotations (2-4 credit hours)
GSMC 852 -Introduction to Biomedical Research (2 credit hours)
GSMC 858 - Introduction to Faculty Research (1 credit hour)
GSMC 850 - Proteins and Metabolism (2 credit hours)
Biochem Foundations in Medicine, Genetics and Neoplasia
KU Course Molecular Biosciences - Protein Structure function course Chaperone Proteins
KU Mini College Course (KU Alumni) - Protein stability and disease.
Outside teaching lectures
UMKC Premedical Scholars Lectureship – two lectures
William Jewell College – Invited teaching lecture – Protein folding and disease.
Missouri State – Protein Folding – Current state of the art
Missouri Western- Bacterial Toxin Lecture.
Students Trained
Past Ph.D. Student Direct Advisor (supervised thesis work directly)
Chee Ming Low – MS – 1999 – Current Senior Scientist position at Pzifer US.
Bryan Tieman – MS – 2001 Current Senior Scientist at Abbott Laboratory
Scott Falke – Ph.D.- 2004 –Current Assoc. Professor in Biology at William
129
Jewell.
Greg Bomhoff – MS- 2007- Current High School Teacher- St Joseph
Hiroo Kayatama – 6 papers 3 first author – Dropped out of Ph.D. programmedical reasons Summer 2012.
Subhashchandra Naik –Ph.D. - 3 first author papers – plus 2 in preparation.
Defended Dec 2012- Current Postdoctoral Fellow in My laboratory
C) Ph.D. Supervisory and Final Dissertation Examination Committee
(Member or Chairperson* for the following Students)
Name
Date Completed on
Campus
Program
Stephen Clum Ph.D.
Fall 1997
Biochemistry and
Molecular Biology
Chee Ming Low* MS
Fall 1997
Biochemistry and
Molecular Biology
LiLin Wang Ph.D.
Fall 1998
Microbiology and
Immunology
Bryan Tieman* MS
Fall 1999
Biochemistry and Molecular
Biology
Fall 2000
Biochemistry and
Molecular Biology
Stephen Parnell Ph.D.
Spring 2001
Biochemistry and
Molecular Biology
Brian Lobo Ph.D.
Fall 2002
KU Pharmaceutical
Chemistry Department
Scott Falke*
Ph.D.
Fall 2003
Biochemistry and
Molecular Biology
Huimin Jiang
Ph.D.
Spring 2004
Biochemistry and
Molecular Biology
Roberto Vanacore* Ph.D.
Fall 2005
Biochemistry and
Molecular Biology
Melissa Khor Ph.D.
Spring 2005
KU Pharmaceutical
Chemistry Department
Alex Dajkovic Ph.D.
Fall 2005
Microbiology and
Immunology
Greg Bomhoff MS.
Spring 2006
Biochemistry and
Molecular Biology
Tim Priddy Ph.D.
Fall 2006
UMKC Biochemistry
Department
Shihyun You
Ph.D.
130
Hiroo Katayama
left due to Medical
reasons 2012
Biochemistry and
Molecular Biology (6
papers)
Subhashchandra Naik
Ph.D.
Spring 2013
Biochemistry and
Molecular Biology
Maurico Uribe-Vargas
2008 - Present
Biochemistry and
Molecular Biology
Alex McDowell
2009- Present
Microbiology and
Immunology
Stephanie Bishop
2011- present
Pharmacology and
Toxicology
Comprehensive examinations (Oral and written) – predoctoral program.*
chair
Name
Date of Comp.
Department
Steven Parnell
Spring 1998
Biochemistry and
Molecular Biology
Shihyun You
Spring 1998
Biochemistry and
Molecular Biology
Susanna Harju*
Spring 1999
Biochemistry and
Molecular Biology
Scott Falke
Spring 2000
Biochemistry and
Molecular Biology
Daniel Kim
Spring 2000
Biochemistry and
Molecular Biology
Roberto Vanacore*
Spring 2001
Biochemistry and
Molecular Biology
Ryan Shultze
Spring 2005
Microbiology and
Immunology
Hiroo Katayama
July 2007
Biochemistry and
Molecular Biology
Shuari Lu
Spring 2008
Biochemistry and
Molecular Biology
131
Subhashchandra Naik
Spring 2009
Biochemistry and
Molecular Biology
Mauricio Vauge Uribe
Spring 2010
Biochemistry and
Molecular Biology
Alex McDowell
Fall 2010
Molecular Biology and
Immunology
Spring 2014
Molecular Biology and
Immunology
Susan Brock
Past Ph.D. Candidate – IGPBS Rotation Students
6 week rotations and lab instruction with Presentations (starting October
2009)
Fall 1992 Stephen Clum presented research results at the Student research
forum in spring 1993,
Fall 1992 one student Xuesong Yuan, included in Journal of Biological Chemistry
article; Fisher, M.T., and Yuan, X. (1994) The Rates of Commitment to
Renaturation of Rhodanese and Glutamine Synthetase in the Presence of the
GroE Chaperonins. The Journal of Biological Chemistry 269, 29598-29601.
Hitao Li Fall 1993;
Simon Low Fall 1994, Joined my laboratory 1 paper
Steven Parnell - Spring 1996, Joined Jim Calvets Laboratory
Byran Tieman Fall 1997 Joined my laboratory – 2 papers,
Scott Falke Fall - 1998
Joined my laboratory 3 papers
Spring 2002 Nick Mueller, Greg Bomhoff. Joined my laboratory 2 papers
Spring 2005 Aron Gottshalk – Joined Jon Conaway Laboratory
Spring 2006 Hiroo Katayama Joined my laboratory 6 papers
Fall, 2006 Jill Morris, Joined Page Gieger laboratory1 paper
Spring 2007 Paul Welch, switched to MD student
Summer 2007 Subhashchandran Naik, -Joined as Graduate student 3 papers 1
in preparation
Summer 2007- MD/Ph.D. Student Bliss Hartnett Publication in Biopolymers,
2010,
Fall 2007 – Mitchell Mcgill, Joined Pharm/tox Publication in J. Functional
132
Genomics 2009.
Fall 2009 Maurico Vega-Uribe Joined Alexey Ladokhin Spring 2010
2010 Julie Mitchell Joined Micro/Immunology dept Publication in Protein Science
2013,
Cassandra Field November 2011, Joined Alexey Ladokhin laboratory
Susan Brock Spring 2012, Joined Micro/Immunology Publication in Biochemistry
2013
Jackie Thompson October – December 2012. Presented at National Meeting
Midwest stress Response, Joined Gerald Carlson Laboratory.
(Additional Note: Not only advising but instruct student to present research at the
end of their rotations 20 min presentation).
Undergraduate students Advised. (summer and/or Fall internships).
Cory Mclain – KU Lawrence Pharmacy School - KBRIN Grant – testing anti
amyloid compounds and examining GroEL effects on amyloid formation. (June
2004).
Ara Azad-Khorassani – KU Edwards campus (January 2005- 2 years) – testing
chaperonin partitioning assay.
Stephen Glaser – University of Kansas - Osmolytes and protein folding
Josh Hamilton – University of Kansas- Osmolytes and protein folding
Kate Prather – William Jewell College- 2 years- completed single ring
purification.
Marek Brzozowski – University of Kansas - Single particle analysis – Network
specialist
Jordan Jurcyk – Washington USingle particle analysis
Andrew Kerns – K- State- GST-PEPCK folding
Derek Low – K- State- GST-PKD purification and folding project.
Nick Degner- University of Kansas - Chaperonin/Osmolyte Screen. 2010-2011
Jack Walker – Washington U.- Nanodisc preparation and protein insertion 2
summers 2011- 2012
Elizabeth Lindboe- Reed College -GroEL detection of antibody Preaggregates 2
summers 2011- 2012
Medical Scholar Advisor 2 summer rotations.
Kirk Sloan – Medical Scholars Grant – testing anti amyloid compounds (July
2004) 1 paper coauthorship published.
Jacob Hodges, Medical Scholars Grant – Chaperonin sink with TTR (July 2006)1 paper coauthorship published.
Outside Research Activities High School students scholars
Research Advisor - Chung Lee (Shawnee-Mission South High School) 12/94133
5/98
Two time Grand Award Winner (as a freshman and Senior) - Kansas City
Science and Engineering Fair
Third Place - National competition
Projects: "The Probing of ATP-Dependent Conformational Changes in GroEL
Using Proteases"
“The role of ATP in the Chaperonin mechanism - A four year study”
Research Advisor – Katie George- High School Teacher - JCCC Tech Prep
Grant 2 weeks July –concentrating folded proteins from chaperonin osmolyte
system.
Research advisor – Richard Kaufman – High School teacher – Normal mode
flexible fitting (See NSF RET funding).
Research Advisor Current – Roshan Bisarya - Olathe North High School –
Osmolyte mixture studies with GFP rescue with GroEL.
Past Postdoctoral Candidates trained
Kirk Smith – Current Assistant Professor
Paul Voziyan – Current Research Associate Professor at Vanderbilt- 6 papers.
Inam Haque – Current research Assistant professor at VA Hospital Kansas City
KS 1 paper
Boris Kornilayev – Current Research Associate at UMKC Biochemistry 1 paper.
Narahari Akkaladevi – July 2011- Sept 2013 - 3 papers published (2 in
preparation)
Srayanta Mukherjee - Dec. 2011 May 2013– 2 papers published 2 separate
papers in preparation.
Current Postdoctoral fellows:
Wendy Lea – April 2013-Current
Divya Amin – September 2013
Subhashchandra Naik- March 2013
PUBLICATIONS:
Naik, S., Brock S., Akkaladevi N., Tally J., Gao P., Zhang N., Pentelute, B. L.,
Collier R. J., Fisher, M. T. (2013) “Monitoring the kinetics of the pH driven
transition of the anthrax toxin prepore to the pore by biolayer inferferometry
and surface plasmon resonance ” Biochemistry Sep 17;52(37):6335-47.
(Biochemistry Journal Website Highlights)
Gogol EP, Akkaladevi N, Szerszen L, Mukherjee S, Chollet-Hinton L, Katayama
H, Pentelute BL, Collier RJ, Fisher MT.(2013) “Three dimensional structure
134
of the anthrax toxin translocon-lethal factor complex by cryo-electron
microscopy.” Protein Sci. 2013 22:586-94 (Cover & Highlights)
Akkaladevi N, Hinton-Chollet L, Katayama H, Mitchell J, Szerszen L, Mukherjee
S, Gogol EP, Pentelute BL, Collier RJ, Fisher MT. (2013) “Assembly of
anthrax toxin pore: lethal-factor complexes into lipid nanodiscs.” Protein Sci.
22:492-501. (in new Methods and Applications paper)
Naik S., Zhang N, Phillip Gao P., and Fisher M.T. (2012) “On the Design of
Broad Based Screening Assays to Identify Potential Pharmacological
Chaperones of Protein Misfolding Diseases.” Current Opinions in Medicinal
Chemistry vol 12:2504-22.
Henriques B.J., Fisher M.T., Bross P., Gomes C.M. (2011) “A polymorphic
position in electron transfer flavoprotein modulates kinetic stability as
evidenced by thermal stress.” FEBS Lett. 585(3):505-10.
Katayama H., Wang J., Tama F., Chollet L., Gogol E.P., Collier R.J., and Fisher
M.T. (2010) “Three-Dimensional Structure of the Anthrax Toxin Pore Inserted
into Lipid Nanodiscs and Lipid Vesicles” Proc. Natl. Acad. Sci. USA. 107,
3453-3457.
Naik S, Haque I, Degner N, Kornilayev B, Bomhoff G, Hodges J, Khorassani AA,
Katayama H, Morris J, Kelly J, Seed J, Fisher MT. (2010) Identifying protein
stabilizing ligands using GroEL. Biopolymers. 93, 237-51.
Katayama H., McGill M., Kearns A., Brzozowski M., Degner N., Harnett B.,
Kornilayev B., Matkovic-Calogovic D., Holyoak T., Calvet J.P., Gogol E.P.,
Seed J., and Fisher MT., (2009) “Strategies for folding of affinity tagged
proteins using GroEL and osmolytes” J. Structural and Functional Genomics.
10, 57-66. epub Dec 2008.
Katayama, H. Janowiak, B.E., Brzozowski, M., Juryck, J., Falke, S., Gogol E.P.,
Collier, R.J., and Fisher, M.T. (2008) “GroEL as a molecular scaffold for
structural analysis of the anthrax toxin pore” Nat. Struc. and Mol. Biol. 15,
754-60. (Featured in Highlights).
Fisher, M.T. (2006) “Proline to the Rescue” Proc. Natl. Acad. Sci. 103, 1326513266.
Bomhoff G., Sloan K., McLain C., Gogol E.P., and Fisher M.T. (2006) "The
effects of the flavonoid Baicalein and osmolytes on the Mg 2+ Accelerated
Aggregation/Fibrillation of Carboxymethylated Bovine 1SS-α-Lactalbumin"
Archives of Biochemistry and Biophysics 453 75-86.
Fisher, M.T. (2006) "Molecular Roles of Chaperones in Assisted Folding and
Assembly of Proteins" Genetic Engineering, Principles and Methods 27, 183220.
Braun CS, Fisher MT, Tomalia DA, Koe GS, Koe JG, Middaugh CR (2005) “A
stopped-flow kinetic study of the assembly of nonviral gene delivery
complexes.” Biophys J. 88, 4146-58.
Falke. S.R., Tama F. Gogol. E.G. and Fisher M.T. (2005) “Structural Changes in
135
GroEL Induced by Protein Substrate Binding J. Mol. Biol.. 348, 219-230.
Voziyan P.A , Johnston M., Chao A., Bomhoff G. and Fisher, M.T. (2005)
“Designing a high throughput refolding array using a combination of the
GroEL chaperonin and osmolytes. J. of Structural and Functional Genomics
6, 183-188 .
George, R., Kelly S.M., Price, N.C., Erbse A., Fisher, M.T. and Lund. P.A. (2004)
“Three GroEL homologues from Rhizobium leguminosarum have distinct in
vitro properties. Biochem. Biophys. Res. Comm. 324, 822-828.
Hui Koon Khor, H.K., Fisher, M.T., and Schoneich C. (2004) “Potential role of
methionine sulfoxide in the inactivation of the chaperone GroEL by
hypochlorous acid (HOCl) and peroxynitrite (ONOO-)” J. Biol. Chem., 279,
19486-19493.
Voziyan Paul and Fisher M.T. (2002) Polyols induce folding of GroEL-bound
bacterial glutamine synthetase without ATP. Archives of Biochemistry and
Biophysics 397, 293-297.
Tieman, B.C., Johnston M. and Fisher, M.T. (2001) “Differences in Chaperonin
Assisted Renaturation of Structurally and Sequentially Homologous Forms of
Malate Dehydrogenase”. J. Biol. Chem. 276 44541-44550.
Liegh-Edwards K, Kueltzo, L.A., Fisher M.T. and Middaugh C.R. (2001)
“Complex Effects of Molecular Chaperones on the Aggregation and Refolding
of Fibroblast Growth Factor-1 ” Archives of Biochemistry and Biophysics 393,
14-21.
Falke S.F., Fisher M.T. and Gogol E.G. (2001) “Classification and
reconstruction of a heterogeneous set of electron microscopic images: A case
study of GroEL-substrate complexes” J. Struct. Biol.133, 203-213.
Falke S.F., Fisher M.T. and Gogol E.G. (2001) “Analysis of GroEL- Glutamine
synthetase complexes by Electron Microscopy” J. Mol. Biol.(cover) 308, 569577
Voziyan P.A. and Fisher M.T. (2000) “GroE-mediated folding of glutamine
synthetase under non-permissive conditions; off-pathway aggregation
propensity does not determine the co-chaperonin requirement” Protein
Science 9, 2405-2415.
Voziyan P.A. Jadhav, L and Fisher M.T. (2000) “ Refolding a glutamine
synthetase truncation mutant in vitro: identifying superior conditions using a
combination of chaperonins and osmolytes.” The Journal of Pharmaceutical
Sciences 89, 1036-1045.
Smith K.E., Voziyan P.A., and Fisher M.T. (1998) “Partitioning of rhodanese onto
GroEL: Chaperonin binds a reversibly oxidized form derived from the native
protein” The Journal of Biological Chemistry 273 28677-28681.
Voziyan P., Tieman B. Low C. M, and Fisher, M.T. (1998) “Initial interactions
between GroE chaperonin complexes with Protein folding intermediates:
Comparison of Different Substrates The Journal of Biological Chemistry
136
273, 25073-25078.
Fisher, M.T. (1998) “GroE chaperonin-assisted folding and assembly of
dodecameric glutamine synthetase Biochemistry (Moscow) Review 63, n4
453-472.
E. Eisenstein, P. Reddy and M. T. Fisher Overexpression, Purification and
Properties of GroES from Escherichia coli in Methods. Enz. Protein Folding:
Catalysts, Accessory Proteins and Chaperones eds George E. Lorimer and
Thomas O. Baldwin (1997) Volume 290 p119- 135.
Smith, K.E. and Fisher, M.T. (1995): Interactions between the groE chaperonins
and Rhodanese: Multiple intermediates and Release and Rebinding. The
Journal of Biological Chemistry 270, 21517-21523.
Fisher, M.T., and Yuan, X. (1994) The Rates of Commitment to Renaturation of
Rhodanese and Glutamine Synthetase in the Presence of the GroE
Chaperonins. The Journal of Biological Chemistry 269, 29598-29601.
Phadtare, S., Fisher, M.T., and Yarbrough, L.R. (1994) Refolding and Release of
Tubulins by a Functional Immobilized groEL Column. Biochem. Biophys.
Acta. 1208, 189-192.
Fisher, M.T., (1994) The Effect of GroES on the GroEL-Dependent Assembly of
Dodecameric Glutamine Synthetase in the Presence of ATP and ADP. The
Fisher, M.T. (1993): On the assembly of dodecameric glutamine synthetase from
stable chaperonin complexes. The Journal of Biological Chemistry 268,
13777-13779.
Fisher, M.T. (1992): Promotion of the in vitro renaturation of dodecameric
glutamine synthetase from Escherichia coli in the presence of GroEL
(Chaperonin-60) and ATP. Biochemistry 31, 3955-3963.
Fisher, M.T. and Stadtman, E.R. (1992): Oxidative modification of E. coli
glutamine synthetase: Decreases in the thermodynamic stability of protein
structure and specific changes in the active site conformation. The Journal of
Biological Chemistry 267, 1872-1880.
Fisher, M.T. (1991): Differences in thermal stability between reduced and
oxidized cytochrome b 562 from Escherichia coli. Biochemistry 30, 1001210018.
Han, M.K., Cyran, F.P., Fisher, M.T., Kim, S.H. and Ginsburg, A (1990):
Xenopus transcription factor IIIA: Evidence for heterogeneity of Zn2+ binding
affinities and specific labeling of cysteine 287. The Journal of Biological
Chemistry 265, 13792-13799.
Shrake, A., Fisher, M.T., McFarland, P.J. and Ginsburg, A (1989): Partial
unfolding of dodecameric glutamine synthetase from Escherichia coli:
Temperature-induced, reversible transitions of two domains. Biochemistry
28, 6281-6294.
Stayton, P.S., Fisher, M.T. and Sligar, S.G. (1988): Determination of
137
cytochrome b 5 association reactions: Characterization of metmyoglobin and
cytochrome P-450 cam binding to genetically engineered cytochrome b 5 . The
Fisher, M.T. and Sligar, S.G. (1987): Temperature jump relaxation kinetics to
the P-450 cam spin equilibrium. Biochemistry 26, 4797-4803.
Fisher, M.T., White, R.E. and Sligar, S.G. (1986): Pressure dissociation of a
protein-protein electron transfer complex. Journal of the American Chemical
Society 108, 6835-6837.
Fisher, M.T. and Sligar, S.G (1985): Control of heme protein redox potential and
reduction rate: Linear free energy relation between potential and ferric spin
state equilibrium. Journal of the American Chemical Society 107, 50185019.
Fisher, M.T. and Sligar, S.G. (1985): Tyrosine motions in relation to the ferric
spin equilibrium of cytochrome P-450 cam . Biochemistry 24, 6696-6701.
Fisher, M.T., Scarlata, S.F. and Sligar, S.G (1985): High pressure investigations
of cytochrome P-450 spin and substrate binding equilibria. Archives of
Biochemistry and Biophysics 240, 456-466.
Book Chapters (not peer reviewed)
Sligar, S., Fisher, M.T., and Carraway, K.(1986): Structure, function, and
fluctuations during P-450 electron transfer. In Cytochrome P-450:
Biochemistry, Biophysics and Induction (Verezkey, L. and Magyar, K., eds.),
Elsevier Developments in Biochemistry, Vol. 27, p. 49.
Murray, R., Fisher, M.T., Debrunner, P. and Sligar, S.(1985): Structure and
chemistry of cytochrome P-450. In Metalloproteins (Harrison, P., ed.), Vol. I,
Macmillan, London, pp. 157-206, 1985.
Fisher, M.T., Scarlata, S. and Sligar, S.(1985): Conformational changes of
cytochrome P-450 cam in relation to substrate binding and ferrin spin equilibria.
In Microsomes and Drug Oxidations (Boobis, A., et al., eds.), Taylor and
Francis, London, pp. 23-32, 1985.
138
UNIVERSITY OF KANSAS SCHOOL OF MEDICINE
Faculty Curriculum Vitae
I.
PERSONAL DATA
First Name
Joseph
Last name
Fontes
Current Academic Rank
Associate Professor
Department(s)
Biochemistry and Molecular Biology
Office Address
KLSIC 1075
Phone
913-588-9848
Fax
913-588-9896
Email
[email protected]
PROFESSIONAL DEVELOPMENT
Undergraduate and Graduate Education
Years (Inclusive)
Degree
Institution
1983-1987
B.S. Pharmacology
University of California,
Santa Barbara
1987-1992 (December)
Ph.D. Pharmacology and
Toxicology
Davis
Postgraduate Education
Years (Inclusive)
Degree
Institution
January 1993 – July 1998
Post-doctoral Fellow
University of California San
Francisco/HHMI
Academic and Professional Appointments and Activities
(List in chronological order. Please explain any discontinuity in professional experience)
Month and Year
Position
Institution
August 1998 – July 2004
Assistant Professor
Cleveland State University
139
August 2004 – December
2006
Associate Professor
Cleveland State University
August 1998 – December
2006
Adjunct Investigator
Lerner Research Institute of
the Cleveland Clinic
Foundation
January 2007 – Present
Associate Professor
University of Kansas Medical
Center
July 2009 – Present
Associate Professor, with
Tenure
Center
Professional Registration/Licensure
Year
Number
State
N/A
Professional Certification(s)
Date
Board
N/A
Professional Societies and Affiliations
Date
Organization (including offices held)
1987-Present
American Association for the Advancement of
Science
1991-Present
American Society of Biochemistry and Molecular
Biology
Honors and Awards (honorary societies, research awards, teaching and other awards)
Year
Award
2009
Student Voice Award, Best Lecturer, Year 1, KUMC
2011
Student Voice Award, Best Lecturer, Year 1, KUMC
2012
Ruth Bohan Teaching Professorship
II. TEACHING ACTIVITIES
(Evaluations and other evidence of quality teaching must be attached; this represents the
140
teacher’s portfolio and should be accurately summarize ALL of your teaching activities.)
See appendix 1
Brief statement of areas of teaching interest:
Though I found the mechanics of teaching at a medical school very different than
my previous experience at a comprehensive university, I have maintained my
general teaching philosophy. Simply stated, I believe my job is to help students see
the world in a new way. That is, my goal is for a student to encounter the world as a
biologist. When approaching a problem, he or she should think like a scientist,
applying the rules of scientific inquiry and his/her fund of knowledge to address that
problem. If a student leaves a course (or series of classes) I have taught with a
collection of facts but without a new way of seeing the world, I have not succeeded.
My philosophy can be illustrated by describing my approach to lecturing in
Foundations of Medicine. My teaching responsibility in Foundations is the “central
dogma” of biology, i.e. DNA structure, repair, replication, transcription and protein
translation. The over-arching theme of these lectures, which I state explicitly in my
very first slide, is that life is “information made animate”. This is simply another way
of saying that genotype is the basis of phenotype, certainly not a revolutionary idea.
But getting students to view human beings as three-dimensional representation of a
digital genetic code is challenging. I strictly adhere to this construct, returning to it
repeatedly as the three-letter acronyms of molecular biology spool out during my
lectures. I want students to view the molecular machinery that goes into maintaining
and expressing DNA as serving the one purpose of creating a proxy (living being) to
test the fitness of information in a given environment. If the information makes a
successful being, it will be passed on. I firmly believe that this goes to the core of
being a physician, that if one is to repair a human being, one needs to truly
understand its biological reason for their existence. Of course this also applies to the
microorganisms with which we interact. Many years after a student forgets what a
given protein complex does, I hope they will retain the idea that the expression and
maintenance of DNA is, on the biological level, the meaning of life.
1. Instruction:
Didactic (e.g.: lectures and formal presentations at KUMC)
Instruction
Student
Academic
Year
Course
Title
2008-2009
Core 800
Foundations
of Medicine
Lecture
10
175
M1
2008-2009
IGPBS
893
Module 3,
Molecular
Biology
Lecture
6
25
Graduate
2008-2009
BCHM
892
Adv
Molecular
Genetics
Lecture
8
5
Graduate
2009-2010
Core 800
Foundations
of Medicine
Lecture
10
175
M1
2009-2010
IGPBS
Module 2,
Molecular
Lecture
6
24
Graduate
141
Type
Hours
No
Type
Biology
2010-2011
Core 800
Foundations
of Medicine
Lecture
10
175
M1
2010-2011
BCHM
892
Adv
Molecular
Genetics
Lecture
6
5
Graduate
2011-2012
Core 800
Foundations
of Medicine
Lecture
10
191
M1
2012-2013
BCHM
892
Adv
Molecular
Genetics
Lecture
6
5
Graduate
2012-2013
Core 800
Foundations
of Medicine
Lecture
15
211
M1
Nondidactic (e.g.: workshops, labs, and discussion groups)
Instruction
Academic
Year
1998-2006
Course
Title
Type
Hours
Student
No
Type
Bio
688/888
Seminar in
Molecular
Biology
Discussion
group
15
3-16
Graduate
Bio 435
Molecular
Biology
Techniques
Laboratory
Laboratory
90
15
Undergrad
2007-2008
Core 800
Foundations
of Medicine
Small
Group
24
22
M1
2007-2008
Core 805
Genetics
and
Neoplasia
Small
Group
12
22
M1
2008-2009
IGPBS
893
Module 3,
Molecular
Biology
Discussion
group
4
22
Graduate
2008-2009
Core 800
Foundations
of Medicine
Small
Group
12
20
M1
2008-2009
IGPBS
893
Module 3,
Molecular
Biology
Discussion
group
4
25
Graduate
(6
semesters)
2000-2001
(2
semesters)
142
2009-2010
Core 800
Foundations
of Medicine
Small
Group
12
24
M1
2009-2010
Core 805
Genetics
and
Neoplasia
Small
Group
12
24
M1
2010-2011
Core 800
Foundations
of Medicine
Small
Group
12
20
M1
2010-2011
Core 805
Genetics
and
Neoplasia
Small
Group
12
20
M1
2011-2012
Core 800
Foundations
of Medicine
Small
Group
12
20
M1
2012-2013
Core 800
Foundations
of Medicine
Small
Group
12
20
M1
2012-2013
Core 805
Genetics
and
Neoplasia
Small
Group
6
20
M1
D.
Master’s Theses and PhD Dissertations directed
Year
Completed
2003
2006
2006
2005
2009
Student Name
Thesis Title
Degree
(Completed/In
process)
Rajini Mudhasani
Regulation of MHC
Class II Gene
Transcription by
BRG-1
Ph.D., completed
(Cleveland State
Univ.)
Wafa Al-Kandari
Regulation of MHC
class II Gene
Transcription by
ZXDC
Ph.D. completed
(Cleveland State
Univ.)
Srikarthika
Jambunathan
Post-translational
modification of the
MHC II gene
regulator, ZXDC
Ph.D. completed
(Cleveland State
Univ.)
The Role of ZXDA in
MHC Class II Gene
Transcription
M.S. completed
(Cleveland State
Univ.)
Vandana Navalgund
Rupa Koneni
143
Regulation of
hematopoietic
transcription factors
Ph.D. completed
by ZXDC
(Cleveland State
Univ.)
Supervision of Postdoctoral Fellows
Year
Fellow Name
Area of Study
2005-2006
Margot Freer, Ph.D.
Regulation of MHC class II gene transcription
2008-2009
Soo-Dong Park, Ph.D.
ZXDC interacting factors and function
2009-
Jon Ramsey, Ph.D.
ZXDC in myelopoiesis
Advising (Thesis or dissertation committees: student academic group/individual) only
KUMC listed
Date
Student or group
name
Type of Student/group
2008-2011
Won-Mee Park
Individual, dissertation committee
2009-2012
Anastasiia
Aleksandrova
2009-
Lu Chen
2009-2011
Aaron Chen
2009-2011
Kyung-Tae Park
2009-2010
Yun Chen
2010-2012
Yi Feng
2010-
Xing Ma
2012-
Allen Chazelle
2012-
Zhen Zhang
2012-
Nairita Roy
2012-
Wahid Mulla
2012-
Min Yao
Other teaching activities
144
Date
Teaching Function
Student
8/21/2009
PhD Comprehensive Exam
Lu Chen
4/8/2009
Yun Chen
and 4/21/2010
Won Mee Park
11/09/2009
Kyung-Tae Park
12/17/2009
Anastasiia Aleksandrova
11/15/2010
Yi Feng
06/15/2011
Xing Ma
08/31/2012
Rachel Grau
10/06/2009
2. Development of Educational Materials
(Course materials e.g. syllabi, educational software packages, web sites, films,
educational tapes and evaluation tools)
Year
Title Description
Intended Audience
2012
Case of the Week Conferences
M1 Medical Students
3. Educational Leadership
(Responsibility for courses and other leadership activities including mentoring of junior
faculty. Please list faculty members mentored – letters from mentorees may be provided.)
Director, CORE 800, Foundations of Medicine. 2008-2008
Member and Vice Chair, Phase I Committee
Faculty Mentor for Liskin Swint-Kruse, Ph.D., Department of Biochemistry and
Molecular Biology
SERVICE ACTIVITIES
See guidelines and instructions to applicants for definitions and suggested documentation of
professional and academic service.
Professional Service:
The diverse area of professional service includes patient care. Applicants should select measures
that most clearly and concisely document their accomplishments and the value of these activities
to the Medical School and University. Measures of both quantity and quality of activities are
required and if necessary, applicants should provide brief descriptions to assist reviewers.
Measures of patient care activities include numbers of patients, time allocation in clinical
activity, procedures completed, Relative Value Units (RVUs), and value to the School of
145
the clinical service. If the primary quality evaluation is the subjective assessment of peers, this
should be available in letters from departmental colleagues, chair, or referees.
The significance of professional service in the forms of task forces, committees and similar
groups should be explained and the specific role of the applicant clarified.
Professional consulting services must have academic credibility and clear service intent and not
be performed primarily for personal profit.
Grant Review Panels:
1. Chair, NIH Special Emphasis Panel (2013/01 ZRG1 GGG-E (90) A Gene Expression
and Regulation Area October 2012
2. Regular Member, NIH study Section Molecular Genetics B (MGB), 2007-2011
3. Ad Hoc Member, NIH Special Emphasis Panel, Academic Research Enhancement
Awards (R15), October 2007
4. American Heart Association, Ohio Valley Affiliate, 2005-2007
5. Environmental Protection Agency, Molecular Biology and Biochemistry, March 2007
Ad hoc manuscript reviewer:
1. Molecular and Cellular Biology
2. Molecular and Cellular Biochemistry
3. Journal of Molecular Biology
4. Journal of Biological Chemistry
5. Biochemical and Biophysical Research Communications
6. BBA-Gene Regulatory Mechanisms
7. Oncogene
Ad hoc external tenure/promotion review
1.
Virginia Commonwealth University, 2012
2.
University of Wisconsin Parkside, 2012
August 2012, Johns Hopkins Press, reviewer biochemistry text book proposal
Academic Service:
In academic service the contribution of the candidate to the academic community should
be clearly documented. Names and dates of committees, task forces, or working groups
should be provided. A concise description of the significance of the group and
explanation of the role of the applicant should be provided. Activities related to
Academic Societies should be documented in this section.
Active:
1. July 2008-Present, Member, Phase I Committee
2. July 2011-Present, Vice-Chair, Phase I Committee
3. Oct 2012-Present, Member, Phase I Curriculum Content Working Group
4. August 2012-Present, Member, LCME Self Study Committee, Student Affairs
5. August 2012-Present, Member, LCME Medical Students Self Study Committee
6. September 2012- Present, Member, Academic and Professionalism Committee
7. September 2012-Present, Member Admissions and MD Student Selection
Committees
8. May 2012-Present, Faculty Advisor, Phi Delta Epsilon Medical Fraternity
9. 2011-Present, Elected At-Large Member, Faculty Assembly
10. 2011-Present, Elected At-Large Member, Faculty Assembly Executive Committee
11. 2010-Present, Faculty Advisor, Delp Society and Career Advising Program
146
Past:
1. January-April 2012, Developed graduation competencies for KU SOM (with Dr.
Anne Walling)
2. August-October 2011, Chair, Renal-Endocrine Module Review Committee
3. 2010-2011, Interviewer, medical student admissions
REARCH and SCHOLARLY ACTIVITIES:
Brief statement of areas of research and scholarly interest, including current projects:
My research interests lie in the regulation of gene transcription, primarily of major histocompatibility complex class II (MHC II) genes. The long term
chromatin modifying complexes involved in activating MHC II gene transcription.
Three years ago we identified a family of novel transcription factors (ZXDA, ZXDB and
ZXDC) involved in the regulation of MHC II genes. Subsequent and current work has
focused on (1) the mechanism by which the ZXD family regulates gene transcription (2)
additional target genes regulated by the ZXD family and (3) the role of ZXDC in
development, particularly hematopoiesis. We now have data indicating that ZXD family
members are involved in macrophage lineage determination, playing an important role in the
development of the immune system. The mechanisms by which the ZXD family regulates
macrophage development, including any potential role in leukemogenesis will be the focus
of my lab in the future. We will also continue to use the ZXD family to study basic aspects of
transcriptional regulation of genes.
1. Grants and contracts
(Information must include whether the nominee is the principal investigator or a coinvestigator, names of all investigators, title of grant, funding source, dollar amount in
direct costs, and years during which grant applies. Co-investigators must specify role).
Provide four copies of abstracts and award notice or renewal for all grants or contracts
awarded in last five years (See Appendix II)
Grants and contracts awarded:
(List in chronological order)
Principal
Investigator
Title of Grant
Funding
Source
Direct
Costs
NIH (NIAID)
$50,000/yr
Fontes
Chromatin
Remodeling and
MHC class II gene
transcription
NIH (NIAID)
Fontes
Regulators and
regulation of MHC
class II gene
transcription
The Role of ZXDC
in Myelopoiesis
NIH (NIAID)
Fontes
NOVEL
MECHANISM FOR
GLUTAMATEDEPENDENT
EXCITOTOXICITY
NIH
(NINDS)
Belousov
Fontes Coinvestigator
(10%)
Grants and contracts submitted:
147
Years
Status
9/20028/2004
Complete
4/200512/2009
Complete
3/20112/2012
Active
7/20126/2014
Active
$175,000/yr
$50,000/yr
$226,500
Principal
Investigator
Investigators
Title of Grant
Funding
Source
Direct
Costs
Years
Status
5
Submitted
Oct
2012/Status
pending
Fontes
Mechanisms of
inflammatory
gene regulation
by the
transcription
factor ZXDC
NIH
(R01)
Fontes
The Role of
Zinc Finger XLinked
Duplicated
(ZXD) Family
Members in
Myelopoiesis
NIH
(R01)
Fontes
Non-coding
RNAs, CTCF
occupancy and
transcriptional
start site
selection
NIH
(R21)
$175,000/yr
2
Fontes
The Role of the
ZXD
Transcription
Factor Family in
Myelopoiesis
KUMC
(Lied)
$35,000
1
Not funded
Fontes
Expression of
the transcription
factor ZXDC in
Acute Myeloid
Leukemia
KUMC
(Cancer
Center)
$35,000
1
Not funded
Fontes
Mechanisms of
Connexin-36
gene
expression and
the
pathophysiology
of diabetes
NIH
(R21)
$175,000/yr
2
Not funded/
Resubmission
pending
Fontes
Inflammatory
gene regulation
by the
transcription
factor ZXDC
AHA
Grantin-Aid
$75,000/yr
2
Scored, not
funded
Fontes
Regulation of
Inflammatory
Gene
Transcription by
ZXDC
NIH
(R01)
$250,000/yr
5
Pending
L. StehnoBittel,
Andrei
Belousov
148
$250,000/yr
Not funded
$250,000/yr
5
Not funded
2.
Scholarly Publications
Full length, peer-reviewed articles in scientific journals: (Provide names of all authors,
year, title, journal, volume, and inclusive pages. List in chronological order.)
Articles published: (Provide 4 copies of each peer-reviewed article published within
the last five years. Other articles may be provided at the applicant's discretion.)
1. Fontes JD, Strawhecker JM, Bills ND, Lewis RE, Hinrichs SH.
Phorbol esters modulate the phosphorylation of human T-cell leukemia virus type I Tax.
J Virol. 1993 Jul;67(7):4436-41.
2. Chang CH, Fontes JD, Peterlin M, Flavell RA.
Class II transactivator (CIITA) is sufficient for the inducible expression of major
histocompatibility complex class II genes.
J Exp Med. 1994 Oct 1;180(4):1367-74.
3. Fontes JD, Jabrane-Ferrat N, Toth CR, Peterlin BM.
Binding and cooperative interactions between two B cell-specific transcriptional
coactivators.
J Exp Med. 1996 Jun 1;183(6):2517-21.
4. Jabrane-Ferrat N, Fontes JD, Boss JM, Peterlin BM.
Complex architecture of major histocompatibility complex class II promoters: reiterated
motifs and conserved protein-protein interactions.
Mol Cell Biol. 1996 Sep;16(9):4683-90.
5. Lim CS, Jabrane-Ferrat N, Fontes JD, Okamoto H, Garovoy MR, Peterlin BM, Hunt
CA.
Sequence-independent inhibition of RNA transcription by DNA dumbbells and other
decoys.
Nucleic Acids Res. 1997 Feb 1;25(3):575-81.
6. Fontes JD, Jiang B, Peterlin BM.
The class II trans-activator CIITA interacts with the TBP-associated factor TAFII32.
Nucleic Acids Res. 1997 Jun 15;25(12):2522-8.
7. Fontes JD, Jabrane-Ferrat N, Peterlin BM.
Assembly of functional regulatory complexes on MHC class II promoters in vivo.
J Mol Biol. 1997 Jul 18;270(3):336-45.
8. Fontes JD, Kanazawa S, Jean D, Peterlin BM.
149
Interactions between the class II transactivator and CREB binding protein increase
transcription of major histocompatibility complex class II genes.
Mol Cell Biol. 1999 Jan;19(1):941-7.
9. Mudhasani R, Fontes JD.
Inhibition of class II trans-activator function by HIV-1 tat in mouse cells is independent of
competition for binding to cyclin T1.
Mol Immunol. 2002 Jan;38(7):539-46.
The class II transactivator requires brahma-related gene 1 to activate transcription of
major histocompatibility complex class II genes.
Mol Cell Biol. 2002 Jul;22(14):5019-26.
Multiple interactions between BRG1 and MHC class II promoter binding proteins.
Mol Immunol. 2005 Apr;42(6):673-82.
12. Al-Kandari W, Jambunathan S, Navalgund V, Koneni R, Freer M, Parimi N,
Mudhasani R, Fontes JD.
ZXDC, a novel zinc finger protein that binds CIITA and activates MHC gene
transcription.
Mol Immunol. 2007 Jan;44(4):311-21.
13. Al-Kandari W, Koneni R, Navalgund V, Aleksandrova A, Jambunathan S, Fontes JD.
The zinc finger proteins ZXDA and ZXDC form a complex that binds CIITA and regulates
MHC II gene transcription.
J Mol Biol. 2007 Jun 22;369(5):1175-87.
14. Jambunathan S, Fontes JD.
Sumoylation of the zinc finger protein ZXDC enhances the function of its transcriptional
activation domain.
Biol Chem. 2007 Sep;388(9):965-72.
15. Aleksandrova A, Galkin O, Koneni R, Fontes JD.
An N- and C-terminal truncated isoform of zinc finger X-linked duplicated C protein
represses MHC class II transcription.
Mol Cell Biochem. 2010 Apr;337(1-2):1-7.
16. Lu J, Wang K, Rodova M, Esteves R, Berry D, Lezi E, Crafter A, Barrett M, Cardoso
S, Onyango I, Parker W, Fontes J, Burns J, Swerdlow R,
Polymorphic Variation in Cytochrome Oxidase Subunit Genes.
150
J Alzheimers Dis. 2010 Jan;21(1):141-54.
17. Wang Y, Denisova JV, Kang KS, Fontes JD, Zhu BT, Belousov AB. Neuronal gap
junctions are required for NMDA receptor-mediated excitotoxicity: implications in
ischemic stroke.
J Neurophysiol. 2010 Dec;104(6):3551-6.
18. Park WM, Wang Y, Park SD, Denisova JV, Fontes JD, Belousov AB. Interplay of
Chemical Neurotransmitters Regulates Developmental Increase in Electrical Synapses
Journal of Neuroscience 2011 Apr 20;31(16):5909-20.
19. Wang Y, Song JH, Denisova JV, Park WM, Fontes JD, Belousov AB. Neuronal gap
junction coupling is regulated by glutamate and plays critical role in cell death during
neuronal injury.
J Neurosci. 2012 Jan 11;32(2):713-25.
20. Song JH, Wang Y, Fontes JD, Belousov AB.
Regulation of connexin 36 expression during development
Neurosci Lett. 2012 Mar 28; 513(1):17-9
21. Belousov AB, Wang Y, Song JH, Denisova JV, Berman NE, Fontes JD.
Neuronal gap junctions play a role in the secondary neuronal death following controlled
cortical impact.
Neurosci Lett. 2012 Aug 22;524(1):16-9.
Manuscripts in press: (Provide names of all authors, title, journal, and 4 photocopies of
manuscript plus evidence of acceptance by journal)
N/A
Manuscripts submitted but not yet accepted: (Provide names of all authors, title, journal,
and 4 photocopies plus evidence of receipt of manuscript by journal.)
Invited or non-peer-reviewed articles or reviews: (Provide names of all authors, year,
title, journal, volume, and pages. If in press, provide documentation and 4
photocopies if published within the last five years.)
1. Fontes, JD, Kanazawa S, Nekrep N, Peterlin BM. The class II transactivator CIITA is
a transcriptional integrator. Microbes Infect. 1999 Sept; 1(11):836-9
2. Nekrep N, Fontes JD, Geyer M, Peterlin BM.
When the lymphocyte loses its clothes.
Immunity. 2003 Apr;18(4):453-7. Review.
3. Fontes, JD and Belousov, AB.
Neuronal gap junctions: making and breaking connections during development and
injury
Trends in Neurosciences 2012 in press
Books and book chapters: (Provide names of all authors, year, book title, chapter
title, edition, publisher, and pages. If in press, provide documentation and 4
photocopies if published the last five years.)
151
1. Hinrichs, S.H., L Chen, J.D. Fontes and G. Jay. Human T-Lymphotropic Virus Type I
(HTLV-I): Studies of disease mechanisms in a transgenic mouse. In: The Human
Retroviruses. Jay, G. and Gallo, R., eds. Academic Press, Washington D.C., 1991
2. Hinrichs SH, J.D. Fontes, N.D. Bills, P.D. Schneider (1993) Transgenic models of
human cancer. Princess Takamatsu Symposia, 1991, 22:259-74.
3.
Presentations and posters
Oral paper presentations: (Provide names of all authors, title, sponsoring organization,
extent of peer-review, and location and date of presentation.)
PLEASE NOTE: Most of my academic career was spent at Cleveland State
University, which is an excellent institution but generally not well funded. As such,
there were essentially no institutional funds for travel. My grant resources were so
precious that I chose to use them entirely to support my lab, rather than on travel.
As such, my participation at scientific meetings was nil.
Invited seminars at other universities: (Provide title, sponsoring organization or
institution, and date of presentation.)
February 2000 Cleveland Clinic Foundation, Dept of Cell Biology
September 2002 University of Toledo, Dept of Biological Sciences
November 2007 Dept of Immunology, Micro and Molec. Genetics, KUMC
September 2008 Rockhurst University, Kansas City, MO
November 2008 Stowers Institute for Medical Research, Kansas City, MO
March 2009 Dept of Anatomy and Cell Biology, KUMC
May 2009 University of North Dakota, Dept of Chemistry and Biochemistry,
Fargo, ND
April 2010 Case Western Reserve University, Dept of Genetics, Cleveland OH
Nov 2010 Northwest Missouri State Univeristy, Dept of Chemistry, Maryville, MO
March 2011 Southern Illinois University SOM, Department of Biochemistry,
Carbondale, IL
November 2011 Cleveland State University, Department of Biological Science,
Cleveland OH
152
CURRICULUM VITAE
Jennifer Gerton
Associate Investigator
Stowers Institute for Medical Research
1000 E. 50th Street
[email protected]
Education
1997
Stanford University, Stanford, CA, Ph.D. Microbiology and Immunology
1990
Stanford University, Stanford, CA, B.A. Human Biology
Positions Held
1/14-present
Investigator, Stowers Institute for Medical Research
1/09-12/13
Associate Investigator, Stowers Institute for Medical Research
7/09-present
Associate Professor, Department of Molecular Biology and Biochemistry,
University of Kansas Medical School
7/02-12/08
Assistant Investigator, Stowers Institute for Medical Research
3/03-6/09
Assistant Professor, Department of Molecular Biology and Biochemistry,
University of Kansas Medical School
8/00-6/02
UCSF Postdoctoral Fellow, topic: chromosome dynamics in S. cerevisiae
Advisor: Joseph DeRisi, Ph.D.
6/97-7/00
UNC Postdoctoral Fellow, topic: DNA recombination and repair in S. cerevisiae
Advisor: Thomas D. Petes, Ph.D.
9/90-6/97
Graduate student, topic: HIV-1 integrase
Advisor: Patrick O. Brown, M.D., Ph.D.
Teaching Experience
2013
SIMR courses, Genomics Module, Transcription and Chromatin Module
2011-2012
SIMR course, Cell division and Chromosome Biology
2009-2012
KU Med IGPBS core curriculum
2004, 2005
Biochemistry 922, Molecular Genetics, KUMed
2003
QB3 Microarray Course Instructor
2001, 2002
Assistant Instructor, Cold Spring Harbor Course “Making and using DNA
microarrays”
153
Research Support
2013
CdLS Foundation grant
2008-2013
NIH R01: Molecular Mechanisms of Chromosome Segregation in Yeast
2007-2009
Hudson Prize
2003-2005
Basil O’Connor March of Dimes Starter Scholar Research Award
2002
1999-2001
Herb Boyer Postdoctoral Fellowship
American Cancer Society Postdoctoral Fellowship
2000
1.
1997-1998
1995
1991-1995
1989-1990
Gordon Conference Travel Scholarship
Juan March Travel Scholarship
UNC Lineberger Cancer Center Postdoctoral Fellowship
Helena Anna Henzl Gabor Travel Scholarship
NIH Training Grant recipient
Howard Hughes Undergraduate Researcher
Professional Associations and Service
2013-2018
Editorial Board, JBC
2012-present
SIMR Travel Award committee
2011-present
CdLS Foundation Research Committee, CdLS Clinical Advisory Board
2011-present
MGA, GCAT NIH study sections, ad hoc
2009
Genetic Mechanisms of Inheritance NSF Panel
2005-present
member, American Society for Cell Biology
2003-present
member, Genetics Society of America
2007-2010
SIMR Microarray Advisory Committee
2004-2010
SIMR Supergroup
2007-2008
Advisory Board for Allen Press
2005-2008
SIMR IACUC
2002-2005
UMKC yeast/fungal group
Workshop Organizer
2013
Ribosome Function and Ribosomopathies, held at SIMR
2011
Ribosome Function and Ribosomopathies, held at SIMR
2004
The Structural Biology of Chromosomes, held at SIMR
Students advised
KU Med students-Ph.D.
graduation
Ray Camahort
2008
154
Matthew Goering
2010
Shuai Lu
2013
Open University students-Ph.D.
graduation
Manjunatha Shivaraju
2011
Karthik Dhatchinomoorthy
expected 2016
Paris VI-Ph.D.
graduation
Musinu Zakari
expected 2014
Stowers graduate school-Ph.D.
graduation
Kobe Yuen
expected 2017
Dissertation committees
KU Med students-Ph.D.
Kristin Watt
Ashleigh Fritz
Lili Pan
Evan Janzen
Aaron Gottschalk
Rushi Trivedi
Open University students-Ph.D.
Guangbo Chen
Dominic Heinecke
Jin Zhu
Stowers graduate schoo-Ph.D.l
Soon-Keat Ooi
Graduate classes taught
2013
SIMR course, Genomics Module
2013
SIMR course, Transcription and Chromatin Module
2011-2012
SIMR course, Cell division and Chromosome Biology
2009-2012
KU Med IGPBS core curriculum
2004, 2005
KU Med Biochemistry 922, Molecular Genetics
Publications
155
Xu B., Lee K.K., Zhang L., Gerton J.L. 2013 Stimulation of mTORC1 with L-leucine rescues
defects associated with Roberts syndrome, PLoS Genet, 9(10):e1003857
Gerton J.L. 2012 Translational mechanisms at work in the cohesinopathies, Nucleus, 3(6), 520-5.
Chen Z., McCroskey S., Guo W., Li H., and Gerton J.L. 2012 A genetic screen to discover
pathways affecting cohesin function in S. pombe identifies chromatin effectors, G3, 2(10), 1161-8.
Shivaraju M., Unruh J.R., Slaughter B.D., Mattingly M., Berman J., Gerton J.L. 2012 Cell cycle
coupled oscillation of centromeric nucleosomes in yeast, Cell, 150 (2), 304-316.
Preview in Cell, 150(2), 245-247.
See Research Highlights in Nature Reviews Genetics and Nature Reviews Molecular Cell
Biology, Aug 2012
Bose T., Lee K.K., Lu S., Xu B., Harris B., Slaughter B.D., Unruh J.R., Garrett A., McDowell W.,
Box A., Li H., Peak A., Ramachandran S., Seidel C., Gerton J.L. 2012 Cohesin proteins promote
ribosomal RNA production and protein translation in yeast and human cells, PLoS Genet,
8(6):e1002749
Shivaraju M. and Gerton J.L. 2011 The dynamics of the Cse4 chaperone Scm3, Cell Cycle
10(22):3823-4.
Shivaraju M., Camahort R., Mattingly M., and Gerton J.L. 2011 Scm3 is a centromeric nucleosome
assembly factor, J Biol Chem, 286 (14):12016-23.
Reviewed in Faculty of 1000
Hewawasam G. and Gerton J.L. 2011. Cse4 gets a kiss-of-death from Psh1, Cell Cycle 10(4):566-7
Hewawasam G., Shivaraju, M., Mattingly M., Venkatesh S., Martin-Brown, S., Florens, L., Workman
J.L. and Gerton J.L. 2010. Psh1 is an E3 ubiquitin ligase that targets the centromeric histone variant
Cse4, Mol Cell, 40(3):444-454.
Preview in Mol Cell, 40(3):351-352
Xiong B., Lu S., and Gerton, J.L. 2010 Hos1 is a lysine deacetylase for the Smc3 subunit of
cohesin, Curr Biology, 20(18):1660-1665.
Lu, S., Goering, M., Gard S., Xiong B., McNairn, A.J., Jaspersen S., and Gerton J.L. 2010
Eco1 is important for DNA damage repair in S. cerevisiae, Cell Cycle 9(16): 3315-3327.
156
Bose T., and Gerton, J.L. 2010 Cohesinopathies, gene expression and chromatin organization, J.
Cell Biol, 189(2): 201-210.
Xiong B., and Gerton, J.L. 2010 Regulators of the cohesin network, Annual Review Biochemistry,
79: 131-153.
Gard S., Light W., Xiong B., Bose T., McNairn, A.J., Harris, B., Fleharty B., Seidel C., Brickner J.,
and Gerton J.L. 2009. Cohesinopathy mutations disrupt the subnuclear organization of chromatin, J.
Cell Biol, 187(4): 455-462.
Featured as Editor’s Choice, Science, 326(5959), Dec 11, 2009
Camahort R., Shivaraju, M., Mattingly M., Li B., Florens L., Nakanishi S., Zhu D., Shilatifard A.,
Workman J. and Gerton J.L. 2009. Cse4 is part of an octameric nucleosome in budding yeast,
Mol Cell 35: 794-805.
McNairn, A.J., and Gerton J.L. 2009. Intersection of ChIP and FLIP: genomic methods to study the
dynamics of the cohesin pathway, Chromosome Research, 17(2):155-63.
McNairn, A.J., and Gerton J.L. 2008. Cohesinopathies: one ring, many obligations, Mutation
Research, 647(1-2):103-11.
McNairn, A.J., and Gerton J.L. 2008. The chromosome glue gets a little stickier, Trends in
Genetics 24(8): 382-389.
Bausch C., Noone S., Henry J.M., Gaudenz K., Sanderson B., Seidel C., and Gerton J.L. 2007.
Transcription alters chromosomal locations of cohesin in S. cerevisiae, Mol Cell Biol
27(24):8522-32.
Camahort, R., Li B., Florens L., Swanson S.K., Washburn M. and Gerton, J.L. 2007. Scm3 is
essential to recruit the histone H3 variant Cse4 to centromeres and to maintain a functional
kinetochore, Mol Cell 26: 1-13.
See Preview in Cell, 129, 1047-1049, 2007
See Research Highlights in Nature Reviews Molecular Cell Biology, Aug 2007
Gerton, J.L. 2007. Enhancing togetherness:Kinetochores and cohesion, Gene Dev 21(3):238-41.
157
Collins K.A., Camahort R., Seidel C., Gerton J.L., and Biggins, S. 2007. The overexpression of
a Saccharomyces cerevisiae centromeric histone H3 variant mutant protein leads to a defect in
kinetochore biorientation, Genetics 175:513-525.
Henry, J.M., Camhort, R., Rice D. Florens, L., Washburn, M. and Gerton, J.L. 2006. Mnd1/Hop2
facilitates Dmc1-dependent interhomolog crossover formation in meiosis of budding yeast, Mol Cell
Biol 26(8): 2913-2923.
Mieczkowski, P. A., Dominska, M., Buck, M. J., Gerton, J.L., Lieb, J. D., Petes, T. D. 2006. A
global analysis of the relationship between the binding of the Bas1p transcription factor and meiotic
recombination activity in Saccharomyces cerevisiae, Mol Cell Biol 26(3):1014-27.
Li, B. Pattenden, S.G., Lee, D., Gutierrez, J., Chen, J., Seidel, C., Gerton, J.L., and Workman, J.L.
2005. Preferential occupancy of histone variant H2AZ at inactive promoters influence local histone
modifications and chromatin remodeling, Proc Natl Acad Sci 102(51):18385-90.
Gerton, J.L. and Hawley, R.S. 2005. Homologous chromosome interactions in meiosis: diversity
amidst conservation, Nat Rev Genet, 6(6):477-87.
Gerton, J.L. 2005. Chromosome cohesion: a cycle of holding together and falling apart, PLoS
Biol, 3(3):e94.
Glynn, E.F., Megee, P.C., Yu, H.G., Mistrot, C., Unal, E., Koshland, D.E., DeRisi, J.L., Gerton, J.L.
2004. Genome-wide mapping of the cohesin complex in the yeast Saccharomyces cerevisiae, PLoS
Biol 2(9):e259.
see News and Views in Nature, 430: 520-521, 2004.
see comment in Journal of Cell Biology, July 2004.
Weber, S.A., Gerton, J.L., Polancic, J.E., DeRisi, J.L., Koshland, D., and Megee, P.C. 2004.
The kinetochore is an enhancer of pericentric cohesin binding, PLoS Biol 2(9):e260.
Gerton, J.L. and DeRisi, J. 2002. Mnd1p: a spliced evolutionarily conserved protein required for
meiotic recombination, Proc Natl Acad Sci 99:6895-6900.
Kearney, H., Kirkpatrick, D., Gerton, J.L., and Petes, T.D. 2001. Meiotic recombination involving
heterozygous large insertions in S. cerevisiae: Formation and repair of large, unpaired DNA loops,
Genetics 158:1457-1476.
158
Gerton, J.L., DeRisi, J., Shroff, R., Lichten, M., Brown, P.O., and Petes, T.D. 2000. Global
mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae.
Proc Natl Acad Sci 97:11383-11390.
Gerton, J.L., Herschlag, D., and Brown, P.O. 1999. Stereospecificity of reactions catalyzed by HIV1 integrase. J Biol Chem 274:33480-33487.
Gerton, J.L., Ohgi, S., Olsen, M., DeRisi, J., and Brown, P.O. 1998. Effects of mutations in residues
near the active site of HIV-1 integrase on specific enzyme-substrate interactions. J Virol 72:50465055.
Gerton, J.L. and Brown, P.O. 1997. The core domain of HIV-1 integrase recognizes key features of
its DNA substrates. J Biol Chem 272:25809-25815.
Ellison, V., Gerton, J., Vincent, K. and Brown, P.O. 1995. An essential interaction between distinct
domains of HIV-1 integrase mediates assembly of the active multimer. J Biol Chem 270: 3320-3326.
Harrington, J., Hsieh, C., Gerton, J., Bosma, G., and Lieber, M. 1992. Analysis of the defect in
DNA end joining in the murine scid mutation. Mol Cell Biol 12: 4758-4768.
Seminar Presentations
2014
Queenstown Research Week, New Zealand
2014
FASEB-Yeast Chromosome structure, replication, and segregation
2014
CdLS National Scientific symposium
2013
Duke University, University Program in Genetics and Genomics
2013
2013
Cohesin Biology and the Cohesinopathies, Certosa di Pontignano, Italy
The Dynamic Nucleus of the Cell: Chromatin, Chromosomes, and Disease, Northwestern
2013
University of Texas, San Antonio, Department of Molecular Medicine
2013
University of Kansas, Edwards, Molecular Biology lab course
2012
University of Virginia, Department of Microbiology, Immunology, and Cancer Biology
2012
EMBO Centromere and Kinetochore workshop
2012
Sanjay Gupta’s visit to SIMR
2012
2012
Cell Cycle, Cold Spring Harbor
2011
NIH, NCI
2011
Memorial Sloan Kettering Cancer Center
2011
Cohesin Biology and the Cohesinopathies, Il Ciocco, Italy
2010
Midwest Yeast Meeting, Northwestern University
159
2010
FASEB Yeast chromosome structure, replication, and segregation
2010
2010
University of Virginia, Symposium on Mechanisms of genome stability
2009
University of Iowa, Department of Biology
2009
2009
2008
2008
Cohesin Biology and the Cohesinopathies, Certosa di Pontignano, Italy
Blaffer Seminar Series, MD Anderson Cancer Center
Dynamic Organization of Nuclear Function, Cold Spring Harbor
Department of Microbiology and Molecular Genetics, UMDNJ
2008
American Society for Biochemistry and Molecular Biology Annual Meeting
2007
American Society for Human Genetics, Cohesinopathies
2007
Transcription, Chromatin, and Cancer, Stowers Institute for Medical Research
2007
Midwest Yeast Meeting, Northwestern University
2007
AWIS meeting, William Jewell College
2006
Dartmouth College, Department of Biological Sciences
2006
FASEB Research Conference on Yeast Chromosome structure, replication, and segregation
2006
University of Rochester, Department of Biology
2006
KUMed, Workshop on Open Access publishing
2006
Bioinformatics 3640, Rockhurst University
2005
Cold Spring Harbor, Eukaryotic Mechanisms of Transcription
2005
International Conference of Yeast Genetics and Molecular Biology
2005
American Society for Microbiology Annual Meeting
2005
William Jewell College
2004
St. Louis University Cancer Center
2004
FASEB Research Conference on Yeast Chromosome structure, replication, and segregation
2004
Gordon Research Conference on Meiosis
2004
Stowers Institute, Structural Biology of Chromosomes Symposium
2004
University of Kentucky, Department of Molecular and Biomedical Pharmacology
2003
University of Kansas Medical School, Department of Biochemistry and Biophysics
2003
University of Missouri, Kansas City
2002
University of Kansas, Lawrence
2002
University of North Carolina, Department of Biology
160
February 2, 2015
III. PERSONAL DATA
First Name
Liskin
Last name
Swint-Kruse
Associate Professor
Department(s)
Office Address
1077 KLSIC
Phone
913-588-0399
Fax
913-588-9896
Email
[email protected]
Undergraduate Education
Years (Inclusive)
Degree
Institution
1986-1990
B.S., Chemistry
1990-1995
Ph.D., Biochemistry
Baylor University, Waco
TX
The University of Iowa,
Iowa
City, IA
Years (Inclusive)
Degree
Institution
1995-1999
Postdoctoral
interdisciplinary studies
in Computational Biology
W. M. Keck Center for
Computational
Biology, Rice
University (Houston
TX) and The
University of Houston
(Houston, TX)
161
2000-2002
Postdoctoral studies in
Biochemistry
Rice University,
Houston, TX
Month and Year
Position
Institution
July 2002 – May 2004
Research scientist
Rice University,
Houston, TX
May 2004 – Sept 2004
Moving to KC;
Sept 2004 – Jun 2009
Advance work for
KUMC
Assistant professor
The University of
Kansas School
of Medicine,
Department of
July 2009 - present
Biochemistry
Courtesy appointment
July 2009 - present
Associate Professor
July 2009 – present
Graduate Director
The University of
Kansas Lawrence,
Department of
Molecular
Biosciences
The University of
Kansas School
of Medicine,
Department of
Biochemistry
Oct 2011 - present
Associate Director,
Interdisciplinary
Graduate Program in
Biomedical Sciences
162
The University of
Kansas School
of Medicine,
Department of
Biochemistry
The University of
Kansas School of
Medicine
Date
1990 – present
2004 – present
American Association for the Advancement of Science
1994 – present
Gibbs Society in Biothermodynamics
2004 – present
Biophysical Society
2004 – present
Protein Society
Year
Award
1986-1990
Welch Scholar (for exceptional students majoring in
Chemistry in the state of TX)
1986-1990
National Merit Scholar
1987
CRC Freshman Chemistry Award (Baylor
University)
1987
National Mathematical Modeling Contest,
Honorable Mention (Baylor University)
1988
Chemistry Department Scholarship (Baylor
University)
1988, 1989
Baylor University Undergraduate Research
Symposium, Winner
1989
Top Scholar, University School (Baylor University)
1990
Phi Beta Kappa (Baylor University)
163
1990
American Institute of Chemists Top Senior
Chemistry Major (Baylor University)
1990
B.S. with Honors (Honors Research Program) and
Magna cum laude (Baylor University)
1990-1994
Iowa Fellow (awarded by The University of Iowa
Graduate College to outstanding Ph.D. applicants)
1991
National Science Foundation Graduate Research
Fellowship, Honorable Mention (The University of
Iowa)
1995-1999
W. M. Keck Computational Biology NLM
Postdoctoral Fellow, Rice University and The
University of Houston
1996
Professor Clarence P. Berg Award for Outstanding
Thesis, Department of Biochemistry, The University
of Iowa
5/2001
6th Annual Structural Biology Symposium (Sealy
Center, UTMB Galveston) Award for best
postdoctoral poster
2002
Gulf Coast Consortium for Bioinformatics/Keck
Center for Computational and Structural Biology
2002 Bioinformatics Symposium. 3rd place poster
award, Rice University
2005-2006
Who's Who in Science and Engineering
2005
KUMC Searle Scholar nominee
164
IV. TEACHING ACTIVITIES
(Evaluations and other evidence of quality teaching must be attached)
I teach basic biochemical principles to M1 students. My goal is for these students
to learn and apply basic principles and reasoning techniques, rather than memorizing long
lists of details. For example, when introducing enzymes, I show the students how they can
(1) learn the 6 basic chemistries carried out by enzymes (and indicated by each enzyme
name) and co-enzymes, and then (2) use that information to rationalize what the reactants
and products of a given reaction should be. I point out that this is not just useful for passing
the STEP exam, but in an era of individualized patient medicine, MDs might receive a list of
altered metabolites and a list of altered proteins for each patient; physicians will need to
make educated correlations between the two lists and rationale hypotheses as to whether
changes could be related to the presenting symptoms. In another example from lectures on
membrane transport, I have these students correlate changes in water, sodium, and glucose
uptake from the gut, in order to understand various causes and remedies for some diarrheas.
In teaching laboratory research skills, such reasoning is equally important. In
general, my strategy has been to allow each student to work as independently as possible,
coming to me when they are “stuck” and giving me their interpretation of results before
hearing mine. This of course depends on their individual progress, and summer
undergraduate students require more guidance (from me or other lab members) than do postdoctoral associates. At all levels, I expect the student to place his or her work in the context
of the larger goal of my lab’s research. Opportunities to show this expertise are given in lab
meetings and summary reports (i.e. that of the American Chemical Society Project SEED
program for economically disadvantaged high school students.) While I design experiments
for high school and undergraduate students, I expect graduate students to assume more
responsibility for experimental design over the course of their training, and post-doctoral
associates even more so. I expect graduate students to write first drafts of their results for
publications, and I expect post-doctoral associates to be full participants in the writing
process.
Student honors:
Dan Parente: Grisolia Award for Excellence in Biomedical Research by a Medical
Student, 2011
165
4. Instruction:
Didactic (e.g.: lectures and formal presentations)
Instruction
Academic
Year
Course
Title
Type
Hours
Student
No
Type
2006-2007
Foundations
of Medicine
Lecturer
Lecture
9
~180
M1
2007-2008
Foundations
of Medicine
Lecturer
Lecture
13
186
M1
2008-2009
Foundations
of Medicine
Lecturer
Lecture
14
181
M1
2008-2009
Foundations
of Medicine
Colecturer
Lecture
“Integration
of concepts”
3
181
M1
2009-2010
Foundations
of Medicine
Lecturer
Lecture
14
177
M1
2009-2010
Foundations
of Medicine
Colecturer
Lecture
“Integration
of concepts”
3
177
M1
2010-2011
Foundations
of Medicine
Lecturer
Lecture
14
179
M1
2010-2011
Foundations
of Medicine
Colecturer
Lecture
“Integration
of concepts”
3
179
M1
2011-2012
Foundations
of Medicine
Lecturer
Lecture
14
198
M1
2011-2012
Foundations
of Medicine
Colecturer
Lecture
“Integration
of concepts”
3
198
M1
2012-2013
Foundations
of Medicine
Lecturer
Lecture
13
216
M1
2012-2013
Foundations
of Medicine
Colecturer
Lecture
“Integration
of concepts”
3
216
M1
166
Instruction
Academic
Year
2005-06
2005-06
2006-07
2006-07
2006-07
Course
Title
Med Biochem
Med Biochem
facilitator
facilitator
Foundations
of Medicine
facilitator
Foundations
of Medicine
facilitator
Genetics/Neo.
facilitator
Type
problembased
learning
4
large group
discussion
4
2007-08
2008-09
2009-10
Genetics/Neo.
facilitator
3
Foundations
of Medicine
facilitator
Foundations
of Medicine
facilitator
167
small group
discussions
M1
M1
~90
M1
Group
1=9
4
2007-08
9
Group
2=9
small group
discussions
small group
discussions
M1
Group
1=9
12
Foundations
of Medicine
Type
Group
2=7
small group
discussions
one-on-one
discussion
No
Group
1=6
20
Foundations
of Medicine
remediation
facilitator
Hours
small group
discussions
2006-07
Student
M1
Group
2=9
1
M1
Group
1=9
16
M1
Group
2=9
Group
1=9
4
M1
Group
2=9
Group
1=9
small group
discussions
8
small group
discussions
8
M1
Group
2=9
Group
1=9
M1
Group
2=9
2009-10
2010-11
2010-11
2011-12
2011-12
2011, fall
2012-13
2012-13
2012, fall
Genetics/Neo.
Foundations
of Medicine
facilitator
facilitator
Genetics/Neo.
Foundations
of Medicine
facilitator
facilitator
Genetics/Neo.
Special
Topics in
Biochemistry
facilitator
Codirector
Foundations
of Medicine
facilitator
Genetics/Neo.
Special
Topics in
Biochemistry
facilitator
Codirector
168
small group
discussions
small group
discussions
small group
discussions
small group
discussions
small group
discussions
Supervise
independent
study for Jason Barnett
small group
discussions
small group
discussions
Supervise
independent
study for
Mary Ashley
Rimmer
Group
1=9
4
M1
Group
2=9
Group
1=8
8
M1
Group
2 = 11
Group
1=8
4
M1
Group
2 = 11
Group
1 = 10
8
M1
Group
2 = 11
Grp 1 =
~10
4
M1
Grp 2 =
~10
6
1
Graduate
Group
1 = 12
8
M1
Group
2 = 12
Group
1 = 12
4
M1
Group
2 = 12
2
1
Graduate
2013,
spring
Special
Topics in
Biochemistry
Director
Supervise
independent
study for
Allen
Chazelle
1
1
Graduate
Year
Student Name
Thesis Title
Degree
(Complet
ed/In
process)
20052006
Cole Stephens
Binding of regulatory
molecules to transcription
regulatory proteins
Student withdrew
2010 present
Daniel Parente
TBD
MD/PhD In progress
Year
2005-2007,
2009
Fellow Name
Hongli Zhan
Area of Study
protein structure/function relationship for
transcription repressors
Advising (Thesis or dissertation committees: student academic group/individual)
Date
Student or group name
2004
Stephanie Fiedler
IGPBS first year rotation
2005
Cole Stephens
KU-Edwards Undergraduate research
2005-2009
Benjamin Weaver
Comprehensive and Ph.D. thesis
committees (BMB, Andrews lab)
2006-2008
Christina Newport
Hester
Ph.D. thesis committee (Micro Immunol,
Lutkenhaus lab)
2006
Melissa Ruhlman
Rockhurst University undergraduate summer
student
2006
Jennifer Lamoureux
student
2006
Tamara Jimenez
2007, 2008
Linh Thai
American Chemical Society Project SEED
169
Summer Research Internship Program for
Economically Disadvantaged High School
Students
2008
Reggie Nguyen
student
2008 - 2012
Kristina Bridges
Zueckert lab)
2008 - 2011
Kendall Smith
committees (MD/PhD in Mol Physiol, Hawley
lab)
2009 - present
Bria Wilkins
committees (Microbiology, KU-Lawrence,
Egan lab)
2009
Mark Wooley
student
2009
Daniel Parente
MD/PhD summer rotation
2009 fall
Renae Springe
Practicum for MS in Molecular
Biotechnology, Dept. of Clinical Laboratory
Sciences
2009 fall
Scott Tan
High school volunteer student
2010 - present
Shuai Lu
Comprehensive and PhD thesis committees
(BMB, Gerton lab)
2010 - present
Veerendra Koppolu
Comprehensive and Ph.D. thesis committee
(Microbiology, KU-Lawrence, Egan lab)
2010 - 2011
Joshua Riepe
Rockhurst University undergraduate student
2010 fall
Evan Janzen
2010 - present
Jacob Hessman
KU-Edwards Undergraduate research
2011 – present
Shishen Du
Lutkenhaus lab)
2012 winter
Jessica Shoop
2012 Feb
Mary Ashley Rimmer
IGPBS extra rotation
2012 spring
Cassandra Field
2012 spring
Allen Chazelle
2012 summer
Bharadwaj Thirumalai
High school student
2012 fall
Jimmy Budjiardo
KU-Lawrence, Program in Bioinformatics,
170
rotation
2012 fall
Beth Jaskiewicz
2013 spring
Matthew Stroh
2013 - present
Saswati Biswas
Comp. exam committee (Micro Immunol,
Yankee lab)
2013 spring
Murial Eaton
Blue Valley ISD High school CAPS program,
independent Biosciences research, outside
mentor (teacher JP Whalen)
Date
Title
Place
Teaching Function
2004-present
presenter
The University of
Kansas Medical
School
Present opportunities
for research in my lab
to 1st year IGPBS
students each fall
semester
Summer 2005
advisor
The University of
Kansas Medical
School
Advise Wyandotte HS
science teacher Mr.
Tyrone Bates on
summer project,
develop adaptations to
use in his classroom
Sept 21, 23,
2005
advisor
The University of
Kansas Medical
School
Met with 9 Wyandotte
HS students to teach
them procedures to be
adapted for their HS
classroom; with
science teacher Mr.
Bates
May 19, 2006
organizer
The University of
Kansas Medical
School
SOM graduate student
meeting with Dr.
Andrew Robertson to
discuss career
opportunities (~15
students)
2006-2007
collaborator
Missouri State
University
Developed content for
a research-based
course with Dr. Colette
Witkowski; lectures
(2006, 2007) to her
class over-viewing the
research project
171
2009-present
Graduate Director
Dept. of Biochemistry
and Molecular
Biology, KUMC
Advise and monitor
progress of BMB
graduate students
Spring 2011
Mentor, grant writing
class
The University of
Kansas Medical
School
Comment on
presentations and
grant application by
graduate student
Subhashchandra Naik
Year
Title Description
Intended Audience
2006
Foundations of Medicine web activity “ELISA”
M1
2007
M1
2007
Foundations of Medicine small group discussion
“Cardiac Biomarkers”
M1
2008
Foundations of Medicine web activity “Weak
acids”
M1
2008
Foundations of Medicine web activity
“Erythrocyte membranes”
M1
2008
M1
2009
acids”
M1
2009
M1
2009
M1
2010
acids”
M1
2010
M1
2010
M1
2010
“Ischemia/Reperfusion”
M1
2010
“Enzymology”
M1
172
2011
acids”
M1
2011
M1
2011
M1
2011
M1
2011
“Enzymology”
M1
2012
Foundations of Medicine web activity “Amino
acids”
M1
2012
acids”
M1
2012
M1
2012
M1
2012
M1
2012
“Enzymology Review”
M1
2012
Foundations of Medicine web activity “Fluid
Mosaic Model”
M1
6. Educational Leadership (Responsibility for courses and other leadership activities
including mentoring of junior faculty. Please list faculty members mentored – letters from
mentorees may be provided.)
Peer teaching evaluation for Charlotte Vines, Ph. D. (KUMC, Dept. of Microbiology and
Immunology), May 2009
Consulting with Sarah Bondos, Ph.D. (Texas A&M Health Science Center) about lecture
materials for teaching first year medical students, summer 2009.
2009-2010 As BMB Graduate Director, led reform of BMB graduate student curriculum in
response to changes in first year IGPBS curriculum.
173
V. SERVICE ACTIVITIES
Academic Service:
National and regional service
Biophysical Society, Committee for Professional Opportunities for Women (2013-present)
Ad hoc manuscript reviewer for the following journals:
Archives of Biochemistry and Biophysics 2009, 2011
Biochemistry 2004, 2005, 2006, 2009, 2011 (2 reviews), 2012 (2 reviews)
Biochimica et Biophysica Acta, 2011
Biophys J 2010. 2012
BMC Microbiology 2008
FEBS Letters (Federation of European Biochemical Societies) 2005
J. Amer. Chem. Soc. 2010
J. Biomolecular Structure and Dynamics 2008
J. Molecular Biology 2007, 2009 (4 reviews), 2010, 2011, 2012 (2 reviews)
Molecular Microbiology 2011, 2012 (2 reviews)
Nucleic Acids Research 2012
PLoS1 2009
pLoS Computation Biology 2013
Protein Engineering, Design, and Selection 2008, 2009
Protein Science 2007, 2010
Proteins: Structure, Function, Bioinformatics 2006, 2008
Trends in Biochemical Sciences 2008
NIH, Macromolecular Structure and Function B study section. 6/23-24/2005. Ad hoc
reviewer (12 proposals)
NSF ad hoc reviewer
2006, Microbial Genetics (2 proposals)
2009, Molecular and Cellular Biophysics (1 proposal)
Cellular Organization (1 proposal)
2011, Molecular and Cellular Biophysics (2 proposals)
174
Netherlands Organisation for Scientific Research, ad hoc reviewer
2009, Division for Chemical Sciences (1 proposal)
Johnson County Community College, Biotechnology Advisory Committee; 5/2006-2009
Twice-yearly committee meetings to advise JCCC on areas related to their
biotech education program
COBRE in Protein Structure and Function (KU-Lawrence)
Protein Purification Core Steering Committee, 2008-present
Hire and oversee director and staff for a facility that provides protein purification
services to the Kansas research community
Conference Organizer
rd
The 23 Annual Gibbs Conference in Biothermodynamics, Oct 3-6, 2009,
Carbondale, IL
Reviewer, National Research Council, Research Associateship Program
May 2011 – 3 proposals
Sept 2011 – 7 proposals
May 2012 – 3 proposals
Sept 2012 – 6 proposals
Dec 2012 – 1 proposal
Outside reviewer for promotion and tenure:
Northern Illinois University, Department of Chemistry and Biochemistry, 2011
Session organizer: “Saturday Night Thermo” for graduate students and post-docs
th
The 25 Annual Gibbs Conference in Biothermodynamics, Sep 17, 2011
th
The 26 Annual Gibbs Conference in Biothermodynamics, Sep 22, 2012
th
The 27 Annual Gibbs Conference in Biothermodynamics, Oct 5, 2013
Judge, student poster presentations.
th
2012 Biochemistry and Cell Biology 40 Anniversary Reunion Symposium, Rice
University, Houston, TX
175
The University of Kansas Medical School service
st
Presentation of BMB research opportunities to 1 year IGPBS students
2004, 2005, 2006, 2007, 2008, 2009, 2010, 2011, 2012
Wrote justifications and participated in purchasing equipment for the Proteomics Center,
2004
Chair, Graduate recruiting committee, 2005-2009
Developed departmental procedure for contacting and tracking prospective
graduate students
Updates to departmental website
Updates to departmental brochure
Recruiting booth at regional American Chemical Society meeting (KC, MO
November, 2007)
Graduate Education Committee, 2005-present
Kimmel Award for Graduate Research, award committee, 2005
Internal grant reviews, 2005-2006
Promotion and Tenure Committee, 2009 - present
Departmental website coordinator, 2010 - present
School of Medicine
Member, junior faculty panel, LCME accreditation visit, 2005
LCME self-study, Medical Student Services subcommittee, 2012
Judge, Student Research Forum
April 2006, 1 section
April 2007, 2 sections
April 2008, 2 sections
176
IGPBS student recruiting weekend.
Presentation (overview of proteomics and structural biology at KUMC) and interviews,
2005
Presentation (overview of proteomics and structural biology at KUMC) and poster,
2007
Presentation (overview of proteomics and structural biology at KUMC), interviews, and
poster, 2008
Recruiting and interviews, 2010
Organized 2012 weekend as IGPBS Assistant Director
Organized 2013 weekend as IGPBS Assistant Director
IGPBS Admissions Committee and interviews, 2009, 2011
IGPBS Advisory Board, fall 2009 - present
MD/PhD student recruiting visits
3 presentations overviewing proteomics and structural biology at KUMC, 2007
3 presentations overviewing proteomics and structural biology at KUMC, student
interviews, 2008
MD/PhD Admissions Committee, fall 2008-present
Guide for KLSIC building tours, Jan 26, 2007
Selection committee for Ruth T Bohan teaching award, 2007
Wahl Society mentor for medical students, 2006-present
Biomedical Research Training Program, application review panel, May 2009, May 2010
WIMS (Women in Medicine and Science) departmental mentoring delegate, 2011present
Department of Pharmacology and Toxicology, Search Committee for Departmental Chair,
2011
177
Academic and Professionalism Committee:
Student Promotions and Special Programs Subcommittee, 2011 – present
Vice chair, 2012-2013
MD/PhD Annual Retreat, July 13, 2012, Presentation on experiences with patents to
students
School of Allied Health
Dept of Clinical Laboratory Sciences, Faculty Search Committee, winter 2007-2008
KUMC
Discussion with BIRCHW* scholars on grant budgeting, June 29, 2012
(*”Building Interdisciplinary Research Careers in Women's Health”)
Panelist for the discussion on Open Access Publishing, Oct 25, 2012
Community service
KC, KS Saturday science club, “Meet the mentor” with high school students, Feb 26, 2005
Westwood View Elementary School Science Night; 2005, 2006, 2007, 2008, 2009, 2010,
2011, 2012, 2013
Develop, supervise facilitators, and lead 8 hands-on activities for ~100 students
Presentations to PEO (a women’s philanthropic educational organization)
Give scientific background for current scientific/ethical issues and lead discussion
April 11, 2006; August 11, 2007; Mar 10, 2009; Apr 2011; March 2012; spring 2013
Blue Valley ISD, Center for Advanced Professional Studies Bioscience Program, speaker
Mar 24, 2011; Oct 10, 2011; Oct 18, 2012
Rockhurst University, Biotechnology course, guest lecturer
Nov 8, 2011
178
VI. RESEARCH and SCHOLARLY ACTIVITIES:
In sequence alignments of protein families, amino acid residues fall into three groups.
Conserved residues convey a common function (e.g. DNA-binding). Nonconserved residues
either fine-tune the distinct functions of homologous family members (e.g. recognition of
different DNA sequences) or are “silent” (mutagenesis has no impact). Understanding the
link between amino acid variation of nonconserved residues and variation in protein function
is important for protein engineering and developing individualized patient therapies. These
applications are impeded by difficulties in discriminating important nonconserved residues
from silent residues. To differentiate these groups, myriad bioinformatics algorithms have
been recently developed. The algorithms rely upon the untested assumption that a given site
falls into a given group for every homologue. Further, the algorithms cannot predict which
aspect of function is altered by each nonconserved position. For example, amino acid
variation in a transcription repressor might alter affinity or selectivity for DNA or allosteric
response to regulatory ligand.
My research bridges bioinformatics, biochemistry, and biophysics to identify important
nonconserved residues. Using transcription repressors, we characterize in vivo function of
multiple variants for multiple homologues. Chimeric proteins are used to simultaneously
facilitate comparisons between homologues and to investigate contributions from residues
that do not directly contact DNA. Results from mutagenesis test predicted locations, discover
trends among homologues, and suggest improved strategies for sequence analyses.
Biophysical experiments delineate which aspect of function is linked to each nonconserved
residue and determine whether these roles are conserved among homologues. Future
studies will extend to other protein families.
A second project is involved in determining whether a protein complex between the Cra
transcription regulator and the FruK enzyme is a novel antibiotic target against γproteobacteria.
In addition to students, my lab has included a number of research scientists: Sarah
Meinhardt, BS (2005-2013); Sudheer Tungtur, MS/MS (2006 - 2012); Ara Azad, BS (2007);
Michael Manley, BS (2009 - present); Renae Springe, BS (2010).
179
4. Grants and contracts
(Information must include whether the nominee is the principal investigator or a coinvestigator, names of all investigators, title of grant, funding source, dollar amount in
direct costs, and years during which grant applies. Co-investigators must specify role).
Provide four copies of abstracts and award notice or renewal for all grants or contracts
awarded in last five years.
Grants and contracts awarded:
(List in chronological order)
Principal
Investigator
Hanzlik,
Robert
(KULawrence)
Investigators
Title of Grant
Michaelis, M
Co-PI (KULawrence)
COBRE:
Protein
Structure and
Function
Swint-Kruse
(Investigator
Subaward)
Funding
Source
Direct
Costs
Years
Status
NIH/NCRR
$300,000
to LSK
9/1/046/30/07
Completed
Jan 2005
Completed
8/1/077/31/12
Funded
Subaward:
Allosteric
determinants
across the
LacI/GalR
family
SwintKruse
KUMC travel
award
KUMC
SwintKruse (PI)
Functional
effects of
exchanging
domains and
linkers in
transcription
regulators
NIH/NIGMS
SwintKruse
KUMC travel
award
KUMC
SwintKruse
ARRA
Administrative
Supplement
to:
Functional
effects of
exchanging
domains and
linkers in
transcription
regulators
NIH/NIGMS
KUMC travel
award
KUMC
(PI)
SwintKruse
180
$350
$900,000
n.c.e. to
7/31/13
$250
Jul
2007
Completed
$179,626
8/1/20097/31/2011
Completed
$300
Feb 2013
Completed
Principal
Investigator
Investigators
Title of Grant
Funding
Source
Direct
Costs
Years
Status
SwintKruse
Intraprotein
signal
transmission in
Venus-flytrap
domains
NIH
$1,125,000
4/1/063/3/11
Not
funded
SwintKruse
Identifying
determinants of
unique function
in a family of
transcription
regulatory
proteins
Searle
Scholars
Program
$240,000
6/1/065/31/08
Not
funded
SwintKruse
Functional
contributions of
domain linkers
in engineered
transcription
regulatory
proteins.
NSF
$552,000
2/1/071/31/12
Not
funded
SwintKruse
Young
Investigator
Award
Howard
Hughes
Medical
Institute
$2,000,000
2009-2015
Not
funded
Transcription
Activation at
the rhaBAD
operon
NIH
$21,000 to
LSK
2011-2015
Oct
2009:
30%ile
Egan,
Susan (KULawrence)
Co-PI
Swint-Kruse
Design and
guide
experiments
of Aim 3
June
2010:
22%ile
Not
funded
SwintKruse
Identification of
functional
properties in
common to
members of a
protein family
NIH
$1,359,507
8/1/20127/31/2017
A0:
Not
funded
A1:
Not
181
funded
SwintKruse and
Aron
Fenton
Swint-Kruse
and Fenton
SwintKruse
Susan
Egan
PI: Joe
Lutkenhaus
Swint-Kruse
Protein
polymorphisms:
Distinguishing
unimportant
from diseasecausing
mutations
NIH
$1,000,000
9/1/20128/31/2016
Not
funded
Experimental
characterization
of
nonconserved
protein
positions: Key
data for
advancing
bioinformatics
analyses
NSF
$720,000
1/1/2013 –
12/31/2016
Not
funded
COBRE:
Novel
Approaches
for Control of
Microbial
Pathogens
NIH
Cost to
LSK
01/01/2013–
06/30/2013
Not
funded
NIH
$1,250,000
9/1/20138/31/2018
In
review
collaborator
Pilot
project:title
SwintKruse
The Cra-FruK
complex: A new
antibiotic target
in gammaproteobacteria
182
5.
year, title, journal, volume, and inclusive pages. List in chronological order.)
Articles published:
Authors
Year
Title
Journal
Vol
Pages
Swint, L. and A. D.
Robertson
1993
"Thermodynamics of unfolding for turkey
ovomucoid third domain: thermal and
chemical denaturation."
Protein Sci
2
2037-49
Swint-Kruse, L. and A.
D. Robertson
1995
"Hydrogen bonds and the pH
dependence of ovomucoid third domain
stability."
Biochemistry
34
4724-32
Swint-Kruse, L. and A.
D. Robertson
1996
"Temperature and pH dependences of
hydrogen exchange and global stability
for ovomucoid third domain."
Biochemistry
35
171-80
Falcon, C. M., SwintKruse, L. and K. S.
Matthews
1997
"Designed disulfide between N-terminal
domains of lactose repressor disrupts
allosteric linkage."
J Biol Chem
272
2681821
Swint-Kruse, L., K. S.
Matthews, P. E. Smith
and B. M. Pettitt
1998
"Comparison of simulated and
experimentally determined dynamics for
a variant of the Lacl DNA-binding
domain, Nlac-P."
Biophys J
74
413-21
Swint-Kruse, L.*, C. R.
Elam, J. W. Lin, D. R.
Wycuff and K. Shive
Matthews
2001
"Plasticity of quaternary structure:
twenty-two ways to form a LacI dimer."
Protein Sci
10
262-76
2002
"Fine-tuning function: correlation of
hinge domain interactions with functional
distinctions between LacI and PurR."
Protein Sci
11
778-94
2003
"Allosteric transition pathways in the
lactose repressor protein core domains:
asymmetric motions in a homodimer."
Protein Sci
12
2523-41
Swint-Kruse, L., H.
Zhan, B. M. Fairbanks,
A. Maheshwari and K.
S. Matthews
2003
"Perturbation from a distance: mutations
that alter LacI function through longrange effects."
Biochemistry
42
1400416
Swint-Kruse, L.
2004
"Using networks to identify fine structural
differences between functionally distinct
Biochemistry
43
10886-
*Corresponding author
Swint-Kruse, L.*, C.
Larson, B. M. Pettitt and
K. S. Matthews
Flynn, T. C.*, SwintKruse, L.*, Y. Kong, C.
Booth, K. S. Matthews
and J. Ma
*Co-first authors
183
protein states."
95
Swint-Kruse, L. and K.
S. Matthews
2004
"Thermodynamics, protein modification,
and molecular dynamics in
characterizing lactose repressor protein:
strategies for complex analyses of
protein structure-function."
Methods
Enzymol
379
188-209
Swint-Kruse, L.* and C.
S. Brown
2005
"Resmap: automated representation of
macromolecular interfaces as twodimensional networks."
Bioinformatics
21
3327-8
*Corresponding author.
Program download has been requested 72
times as of Feb 15, 2011.
2005
"Integrated insights from simulation,
experiment, and mutational analysis
yield new details of LacI function."
Biochemistry
44
1120113
Zhan, H., Swint-Kruse,
L. and K. S. Matthews
2006
"Extrinsic interactions dominate helical
propensity in coupled binding and
folding of the lactose repressor protein
hinge helix."
Biochemistry
45
5896906
Kolin, A., V. Jevtic,
Swint-Kruse, L. and S.
M. Egan
2007
"Linker regions of the RhaS and RhaR
proteins."
J Bacteriol
189
269-71
Tungtur, S., S. M. Egan
and L. Swint-Kruse
2007
"Functional consequences of
exchanging domains between LacI and
PurR are mediated by the intervening
linker sequence."
Proteins
68
375-88
Wilson, C. J., H. Zhan,
L. Swint-Kruse and K.
S. Matthews
2007
"Ligand interactions with lactose
repressor protein and the repressoroperator complex: the effects of
ionization and oligomerization on
binding."
Biophys Chem
126
94-105
Swint-Kruse, L. and H.
F. Fisher
2008
"Enzymatic reaction sequences as
coupled multiple traces on a
multidimensional landscape."
Trends
Biochem Sci
33
104-12
Taraban, M., H. Zhan,
A. E. Whitten, D. B.
Langley, K. S.
Matthews, L. SwintKruse* and J.
Trewhella*
2008
"Ligand-induced conformational changes
and conformational dynamics in the
solution structure of the lactose
repressor protein."
J Mol Biol
376
466-81
Swint-Kruse, L.*, H.
Zhan* and K. S.
Matthews
*Co-first authors
*Co-corresponding
authors
184
Zhan, H., M. Taraban, J.
Trewhella and L. SwintKruse
2008
"Subdividing repressor function: DNA
binding affinity, selectivity, and allostery
can be altered by amino acid
substitution of nonconserved residues in
a LacI/GalR homologue."
Biochemistry
47
8058-69
Meinhardt, S. and L.
Swint-Kruse
2008
"Experimental identification of specificity
determinants in the domain linker of a
LacI/GalR protein: Bioinformatics-based
predictions generate true positives and
false negatives."
Proteins
73
941-57
Tungtur, S., Meinhardt,
S., and Swint-Kruse, L.
2010
“Comparing the functional roles of
nonconserved sequence positions in
homologous transcription repressors:
Implications for sequence/function
analyses.”
J. Mol Biol
395
785-802
Tungtur, S., Parente,
D.J., and Swint-Kruse,
L.
2011
“Functionally Important Positions Can
Comprise the Majority of a Protein’s
Architecture.”
Proteins
79
15891608
Tungtur, S., Skinner, H.,
Zhan, H.L., SwintKruse*, L., Beckett, D.*
2011
In vivo tests of thermodynamic models of
transcription repressor function.
Biophysical
Chemistry
159
142-151
2012
Novel insights from hybrid LacI/GalR
proteins: Family-wide functional
attributes and biologically significant
variation in transcription repression.
Nucleic Acids
Research
40
11139-54
*Co-corresponding
authors
Meinhardt, S., Manley,
M.W. Jr., Becker, N.A.,
Hessman, J.A., Maher,
J.L. III, and SwintKruse, L.,
Manuscripts in press: (Provide names of all authors, title, journal, and 4 photocopies of
Authors
Title
Journal
Manuscripts submitted but not yet accepted: (Provide names of all authors, title, journal,
and 4 photocopies plus evidence of receipt of manuscript by journal.)
Authors
Title
185
Journal
Invited or non-peer-reviewed articles or reviews: (Provide names of all authors, year,
title, journal, volume, and pages. If in press, provide documentation and 4
photocopies if published within the last five years.)
Authors
Year
Matthews, K. S., C. M.
Falcon and SwintKruse, L.
2000
Wilson, C. J., H. Zhan,
L. Swint-Kruse and K.
S. Matthews
2007
Swint-Kruse, L.* and
Matthews, K. S.
2009
Title
Journal
"Relieving
repression."
Vol
Nature Structural Biology
"The lactose
repressor system:
paradigms for
regulation, allosteric
behavior and protein
folding."
Allostery in the
LacI/GalR Family:
Variations on a
Theme
Cell Mol Life Sci
Current Opinion in
Microbiology
Pages
7
184-7
64
3-16
12
129137
title, edition, publisher, and pages. If in press, provide documentation and 4
photocopies if published the last five years.)
Authors
Year
Book Title
Bondos, S.,
Swint-Kruse,
L., &
Matthews, K.
2000
McGraw-Hill
Yearbook of
Science and
Technology
2000
“Protein
Folding”
Swint-Kruse,
L. and
Matthews K.
S.
2004
Encyclopedia
of Biological
Chemistry
“Gene
expression
in bacterial
systems: lac
operon.”
Swint-Kruse,
L in: Krebs,
JE,
Goldstein,
ES. and
Kilpatrick,
ST, eds
2011
Lewin's Genes
X
Chapter
Editor for
Chapter 26:
“The
Operon”
186
Chapter Title
Edition
Publisher
Pages
Licker, M.,
publisher,
McGraw-Hill,
New York
361363
2
W.J. Lennarz
and
M.D.Lane,
eds.,
Academic
Press, San
Diego, CA.
529535
10
Jones and
Bartlett,
publishers,
Sudbury,
MA
pp
735766.
Swint-Kruse,
L. and
Matthews K.
S.
2012
Encyclopedia
of Biological
Chemistry
Revisions to:
“Gene
expression
in bacterial
systems: lac
operon.”
Volume
2
W.J. Lennarz
and
M.D.Lane,
eds.,
Academic
Press, San
Diego, CA.
pp
529534
Published abstracts: (Provide names of all authors, year, title, where published, volume,
and pages.)
Authors
Year
Title
Where published
Volume
Pages
Swint, L. and A. D.
Robertson
1994
"Correlation of global and local
rd
stabilities of ovomucoid 3
domain."
Biophysical Journal
66
A181
SwintKruse, L., B. M.
Pettitt and K. S.
Matthews
1997
"Design and tests of
conformational change for a
transcriptional repressor protein."
Biophysical Journal
72
TH270
Zhan, H. L., L. SwintKruse, B. Fairbanks
and K. S. Matthews
2003
"Mutations that affect allosteric
transition in lactose repressor."
Biophysical Journal
84
501A
Matthews, K. S., L.
Swint-Kruse, J.
Barry, C. Falcon, J.
P. Ma and H. L. Zhan
2004
"Lactose repressor protein
allosteric behavior: Role of
flexibility."
Biophysical Journal
86
354A
Zhan, H. L., L. SwintKruse and K. S.
Matthews
2004
"Mutations that affect allosteric
regulation in LacI."
Biophysical Journal
86
81A
Zhan, H. L., L. SwintKruse and K. S.
Matthews
2005
"Blocking the path: Hydrophobic
mutations at position 84 impair
inducibility in the lactose
repressor protein."
Biophysical Journal
88
220A221A
Swint-Kruse, L., H. L.
Zhan, S. Tungtur and
S. Meinhardt
2007
"Functional effects of exchanging
domains between homologous
proteins are mediated by the
linker."
Biophysical Journal
Supplement S
219A-
Taraban, M., H. L.
Zhan, K. Matthews,
L. Swint-Kruse and J.
Trewhella
2007
"Small angle scattering studies of
LacI repressor."
Biophysical Journal
Supplement S
52A
187
Swint-Kruse, L.,
Zhan, H., and
Tungtur, S
2008
“Determinants of unique
repressor function”
Biophysical Journal
94
Swint-Kruse, L.,
Manley, M., Tungtur,
S., and Zhan, H.L
2011
Correlating in vitro measurements
of protein-DNA binding affinities
with in vivo repression and impact
on the growth rate of the host
organism.
Biophysical Journal
100(3),
S1
321a
Swint-Kruse, L., and
Parente, D.J.
2012
Multiple co-evolutionary networks
have evolved on the common
tertiary scaffold of the LacI/GalR
proteins.
Biophysical Journal
102(3),
S1
184a
Swint-Kruse,
Tungtur, Zhan,
Becker, Maher, and
Riepe
2013
In vitro thermodynamics of DNA
binding correlate with in vivo
transcription repression by a
synthetic LacI/GalR paralog
Biophysical Journal
102(4)
xx
Supplement
S1
Other scholarly publications
N/A
6.
Pos 2722
Presentations and posters
Oral paper presentations: (Provide names of all authors, title, sponsoring organization,
extent of peer-review, and location and date of presentation.)
Scientific papers presented at national and international meetings:
Since September, 2003
Asymmetric Allosteric Transition Pathway in the LacI Core Domain Homodimer
th
17 Annual Gibbs Conference in Biothermodynamics, Carbondale, IL,
Sept 29, 2003.
Invited by organizers.
Subdividing repressor function: DNA binding affinity, selectivity,
and allostery can be altered by amino acid substitution of nonconserved
residues in a LacI/GalR homologue
22
nd
Annual Gibbs Conference in Biothermodynamics, Carbondale, IL,
October 5, 2008
Invited by organizers.
Subdividing repressor function.
th
64 Calorimetry Conference, Santa Fe, NM, Jun 28-Jul 2, 2009. Invited
by organizers.
188
Comparing the functional roles of nonconserved sequence positions in
homologous transcription
repressors: Implications for sequence/function analyses
Keystone symposium, Structural Genomics, Breckenridge, CO. Jan 10,
2010. Invited by
organizers from submitted abstract.
Bringing Science to the Layperson.
th
09/27/10 The 24 Annual Gibbs Conference on Biothermodynamics,
Carbondale, IL.
Chosen from submitted abstracts.
Experimental Description of a Protein Family: Critical experiments for the postgenomic era
th
Carbondale, IL.
Invited by the organizers.
Scientific papers presented at local and regional meetings:
N/A
Poster presentations:
At national and international meetings:
From April 2004
2/12/05-2/16/05 Biophysical Society 49th Annual Meeting, Long Beach, CA.
Peer-reviewed.
1. “Blocking the path: Hydrophobic mutations at position 84 impair
inducibility in the lactose
repressor protein.” Zhan, Swint-Kruse,
and Matthews. (1076-Pos)
2. “Using networks to identify functionally relevant differences
between similar protein
structures.” Swint-Kruse, (posL37)
7/30/05-8/03/05 19th Symposium of the Protein Society, Boston, MA. Peerreviewed.
“RESMAP: Automated Representation of Protein Interfaces as TwoDimensional Networks Aids
Detailed Comparison of Multiple
Structures.” Swint-Kruse and Brown. (abstract #560).
189
th
2/18/06-2/22/06 Biophysical Society 50 Annual Meeting, Salt Lake City, UT.
Peer-reviewed.
1. Functional consequences of domain swapping between the LacI/GalR
transcription regulatory
proteins. Swint-Kruse, Zhan, Meinhardt, and Tungtur
(1671-Pos)
2. Extrinsic interactions dominate helical propensity in coupled binding
and folding of lactose
repressor protein hinge helix. Zhan, SwintKruse, and Matthews. (2434-Pos)
th
10-10/06 20 Annual Gibbs Conference in Biothermodynamics, Carbondale, IL.
Peer reviewed.
“Functional Consequences of Exchanging domains between LacI and
PurR: The linker sequence
modulates DNA-binding in a manner
that is context-dependent upon the regulatory domain.”
Swint-Kruse,
Zhan, Tungtur, and Meinhardt.
st
3/2/2007-3/7/2007 Biophysical Society 51 Annual Meeting, Baltimore, MD.
Peer reviewed.
1. Functional effects of exchanging domains between homologous
proteins are mediated
by the linker; Swint-Kruse, Zhan, Tungtur,
Meinhardt (1027-Pos).
2. Small Angle Scattering Studies of LacI Repressor; Taraban, Zhan,
Woodward, Swint-Kruse,
Matthews, and Trewhella (238-Pos).
7/21/2007 - 7/26/2007. American Crystallographic Association Annual Meeting,
Salt Lake City, UT.
Peer-reviewed. Small Angle Scattering Studies of LacI Repressor.
Taraban, Zhan, Matthews,
Swint-Kruse, and Trewhella
st
7/21/2007-7/25/2007 21 Symposium of the Protein Society, Boston, MA. Peer
reviewed.
Modifying function in engineered transcription repressors. Swint-Kruse
and Meinhardt.
st
9/29/2007 - 10/2/2007 21 Annual Gibbs Conference in Biothermodynamics,
Carbondale, IL.
Peer reviewed. Determinants of Unique Repressor Function. SwintKruse, Zhan, Tungtur,
Taraban, and Trewhella.
nd
2/6/2008 Biophysical Society 52 Annual meeting, Long Beach, CA. Peer
reviewed.
Determinants of Unique Repressor Function. Swint-Kruse, Zhan, and
Tungtur (2722-Pos).
06/11/08 – 06/12/08 Gordon Conference in Biopolymers, Newport, RI. Peer
reviewed.
Determinants of Unique Repressor Function. Swint-Kruse.
01/08/10 Keystone Symposium: Structural Genomics. Peer reviewed.
Comparing the functional roles
of nonconserved sequence positions in
homologous transcription repressors: Implications for
sequence/function analyses. Tungtur, Meinhardt, and Swint-Kruse.
th
Carbondale, IL. Peer reviewed.
Correlating in vitro
190
measurements of protein-DNA binding affinities with in vivo repression
and impact on the growth rate of the host organism. Manley, Tungtur, and Swint-Kruse.
th
Carbondale, IL. Peer reviewed.
Comparison of the coevolution networks between subfamilies of the LacI/GalR transcription
repressor family. Parente, Tungtur, and Swint-Kruse.
th
3/7/2011 Biophysical Society 55 Annual meeting, Baltimore, MD. Correlating in
vitro measurements of
protein-DNA binding affinities with in vivo repression and impact on
the growth rate of the
host organism. Manley, Tungtur, Zhan, and Swint-Kruse.
th
Comparison of co-evolutionary
network structure between subfamilies of the LacI/GalR protein
family. Parente and SwintKruse.
th
09/18/11 The 25 Annual Gibbs Conference on Biothermodynamics, Functional
contributions of
nonconserved amino acids to homologs. Swint-Kruse and Tungtur.
01/15/12 – 1/20/12 Gordon Conference on Biomolecular Interactions and Meth.,
Galveston, TX,
Protein polymorphisms at nonconserved positions give rise to
changes in in vitro protein-DNA
binding affinities that correlate with altered in vivo repression and
organism growth rates.
Swint-Kruse, Manley, Zhan, and Tungtur
th
02/26/12 Biophysical Society 56 Annual meeting, Multiple co-evolutionary
networks have evolved on
the common tertiary scaffold of the LacI/GalR proteins. Swint-Kruse
and Parente
th
Carbondale, IL. Experimental tests
for the existence of neutral positions in the LacI/GalR family. Parente
and Swint-Kruse
02/06/13 Biophysical Society 57th Annual meeting, Philadelphia, PA. In vitro
thermodynamics of DNA binding correlate with in vivo transcription
repression by a synthetic
LacI/GalR paralog. Swint-Kruse, Tungtur, Zhan, Becker, Maher, and
Riepe.
191
06/16/13 – 06/21/13 Gordon Conference on Proteins, Holderness, NH.
Nonconserved amino acids as rheostats for modifying transcription
repressor proteins. Swint-Kruse, Meinhart, Manley, and Tungtur.
Poster presentations at local and regional meetings:
From April 2004
11/18/04 Faculty Research Day, KUMC, Kansas City, KS. Not peer reviewed.
“Tools to identify functionally relevant fine details in protein structures.”
Swint-Kruse
Fall, 2005 Faculty Research Day, KUMC, Kansas City, KS. Not peer reviewed.
“RESMAP: Automated Representation of Protein Interfaces as TwoDimensional Networks Aids
Detailed Comparison of Multiple
Structures.” Swint-Kruse and Brown
9/30/2006. Midwest Enzyme Chemistry Conference. Chicago, IL. Peer
reviewed.
“Enzymatic reaction sequences as coupled multiple traces on a multidimensional landscape”
Fisher and Swint-Kruse
11/7/2007 - 11/9/2007. Midwest Regional American Chemical Society meeting.
Kansas City, MO
Peer reviewed. Determinants of Unique Repressor Function. SwintKruse, Zhan, Tungtur,
Taraban, and Trewhella
11/06/2008 Faculty Research Day, KUMC, Kansas City, KS. Not peer reviewed.
Determinants of Unique
Repressor Function. Swint-Kruse, Zhan,
Meinhardt, and Tungtur
11/03/2009 Faculty research day, KUMC. Not peer reviewed. Comparing the
functional roles of
nonconserved sequence positions in homologous transcription
repressors: Implications for
sequence/function analyses. Tungtur,
Meinhardt, and Swint-Kruse
10/12/10 KUMC, Faculty Research Day. Correlating in vitro measurements of
protein-DNA binding
affinities with in vivo repression and impact on the growth rate of the host
organism. Manley,
Tungtur, and Swint-Kruse
10/07/12 Symposium on Protein Structure and Function, in conjunction with the
10th anniversary of
the COBRE in Protein Structure and Function, KU-Lawrence.
Multiple co-evolutionary
networks have evolved on the common tertiary scaffold of the
LacI/GalR proteins. Parente
192
and Swint-Kruse.
10/12/12 Biochemistry and Cell Biology 40th Anniversary Reunion Symposium,
Rice University,
Houston, TX. Protein polymorphisms at nonconserved positions give
rise to changes in in
vitro protein-DNA binding affinities that correlate with altered in vivo
repression and
organism growth rates. Swint-Kruse, Meinhardt, Tungtur, Zhan, and
Manley
Invited seminars at other universities:
Since October, 2003
Tools to identify functionally relevant fine details in protein structure.
W. M. Keck Center for Computational Biology Seminar Series, Houston,
TX, Spring 2004
KU Med. School, Department of Biochem. Mol. Biol. Seminar Series,
Sept 9, 2004
Using Networks to Identify Relevant Differences in Related Protein Structures.
KU-Lawrence COBRE in Protein Structure and Function, Jan 12, 2005
Understanding Allostery in Transcription Regulatory Proteins: Integrating
Simulation, Experiment, and
Mutational Analyses.
University of Missouri – Kansas City, School of Biological Sciences, Oct
27, 2005
Post-genomic Challenges in Understanding the Protein Structure/Function
Relationship.
Missouri State University, Biomedical Sciences Departmental Seminar
Series, Apr 28, 2006
Functional effects of exchanging domains and linkers in transcription regulators
KU-Lawrence COBRE in Protein Structure and Function, Nov 15, 2006
Identifying Functional Specificity Determinants for a Family of Transcription
Regulators
Kansas State University, Biochemistry Departmental Seminar Series,
Feb 14, 2007
Bridging Bioinformatics and Protein Engineering: The Functional Effects of Amino
Acid Polymorphisms
Pittsburg State University, Chemistry Departmental Seminar Series, Nov
30, 2007
193
Bridging Bioinformatics and Biochemistry to Engineer Unique Repressor
Functions
Texas A&M Health Science Center, College Station, TX, Department of
Molecular and Cellular
Medicine, Dec 2, 2008
University of Kansas, Lawrence, KS, Department of Molecular
Biosciences, Jan 26, 2009
University of Texas Medical Branch, Galveston, TX, Department of
Biochemistry and Molecular
Biology, Jan 29, 2009
UT Health Science Center, San Antonio, TX, Department of
Biochemistry, Sep 11, 2009
Using bioinformatics and protein engineering to elucidate the functional impact of
protein polymorphisms
Kansas State University, Manhattan, KS, Department of Biochemistry,
Feb 10, 2010
Functional Effects of Amino Acid Polymorphisms: Implications for Individualized
Patient Therapies and Protein Engineering
Notre Dame University; Department of Chemistry and Biochemistry, Nov
5, 2010
Park University; Chemistry and Biology clubs, Nov 9, 2010
Wichita State University, Department of Chemistry, Nov 16, 2011
Experimental Description of a Protein Family: Critical experiments for the postgenomic era
Rice University, Department of Biochemistry and Cell Biology, May 14,
2012
Louisiana State University, Department of Biological Sciences, Nov 5,
2012
The LacI/GalR bacterial transcription regulators:
surprises from experimental bioinformatics
Metabolic
The University of Kansas Medical Center, Department of Microbiology,
Molecular Genetics,
and Immunology, Feb 12, 2013
194
7.
Other evidence of scholarship
(includes clinical guidelines, policy documents, contributions to significant position
statements by professional organizations, and development of national examinations)
Patent: Matthews, K. S., Falcon, C. M., and Swint-Kruse, L. Lactose repressor proteins
with increased operator DNA binding affinity. Filed 7/2002 and approved 4/16/2008.
Application number 10/197,053.
195
Date: May 13, 2013
I.
PERSONAL DATA: Instructions available online.
1. Applicant Information:
First Name: Alexey
Middle Initial: S
Last Name: Ladokhin
Suffix:
Degree(s): Ph.D., D.Sc.
Current Academic Rank: Associate Professor
Primary Department: Biochemistry and Molecular Biology
Secondary Department:
Office Address: 1073 KLSIC
Phone: 913-588-0490
Fax: 913-588-9896
Email: [email protected]
2. Professional Development: Instructions available online.
Undergraduate and Graduate Education:
Years (inclusive)
Degree
BS (Physics)
1979-1984
Ph.D. (Biophysics)
1984-1989
Institution
Shevchenko National Univ.,
Kiev
Inst. of Biochemistry,
NANU, Kiev
Postgraduate Education:
Years (inclusive)
Degree
1990-1992
Postdoctoral Research
Associate
1992-1994
Postdoctoral Fellow
1994-2000
Post-Graduate Researcher
Institution
University of Virginia,
Charlottesville, VA
Johns Hopkins University,
Baltimore, MD
Irvine, CA
Academic and Professional Appointments and Activities:
196
Month and Year
Position
Institution
Asst. Biophysicist
(Equivalent of Research
Assistant Professor)
2000-2004
2004-2008
Irvine, CA
Assistant Professor
2008-present
Univ. of Kansas School of
Medicine, Kansas City, KS
Associate Professor
Univ. of Kansas School of
Professional Registration/Licensure:
Year
Number
Professional Certification(s):
Date
State
Board
Professional Societies and Affiliations: List by national and local level; include
offices held
Date
Organization
1989 – present
Ukrainian Biochemical Society
2011 - present
1990 – present
American Biophysical Society
Honors and Awards:
Year
1984
Award
Diploma with Honors in Molecular Physics, Shevchenko
National University, Ukraine
Best Young Scientist Award, Institute of Biochemistry,
Kiev, Ukraine
1988
1992
McCollum-Pratt Institute Fellowship, The Johns
Hopkins Univ., Baltimore, MD
2002
Doctor of Science Degree, National Academy of
Sciences of Ukraine, Kiev, Ukraine
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II.
TEACHING ACTIVITIES: Instructions available online.
Teaching evaluations and other evidence of quality teaching must be provided and
should accurately summarize ALL of your teaching activities. The absence of teaching
evaluations may adversely affect your application for Promotion and/or Tenure.
Philosophy of Teaching:
Briefly describe your philosophy of teaching and how you apply it to different types of
learners, e.g. medical students, graduate students, residents, fellows.
Although I define my field as molecular biophysics, my research is interdisciplinary
and draws upon my knowledge of biochemistry and molecular biology, as well as
chemistry and physics. So my teaching has to be multidisciplinary too. My
expertise in these fields makes me confidently eager to teach courses as diverse as
biochemistry, biophysics, protein structure and thermodynamics, physical methods
in biomedical sciences, and membrane protein structure and function. I believe that
students should be taught the very latest scientific concepts and that they should be
exposed to an historical perspective on current theories (I implement these aspects
in my graduate courses). The most important and difficult challenge in teaching is
not so much imparting knowledge as demonstrating effectively how that knowledge
is obtained from observation, experiment, and analysis.
1. Instruction:
Didactic: e.g. lectures and formal presentations
Instruction
Academic
Year
2003
2005 2009
2006 present
2012 present
Title and
Course
Number
Hours
(actual
instruction)
Number
of
Learners
6
8
Lecture
10-14
23-27
Protein
StructureFunction
Lecture
12
3
Methods for
Analyzing
Biomolecules
Lecture
12
5
Physiology
204
IGPBS
Module 1
BCHM 923
198
Lecture or
Presentation
Title
Type (e.g.
lecture,
grand
rounds,
formal
presentation)
Learner
Concepts of
Biophysics
Lecture
Type of
Learner(s)
(e.g.
medical
students,
graduate
students,
residents)
Graduate
Students
Graduate
Students
Graduate
Students
Graduate
Students
2012 present
BCHM 808
Biochemistry
Res-Lit
Seminar
Seminar
15
8
Graduate
Students
BCHM 862
Nondidactic: e.g. workshops, labs, and discussion groups
Instruction
Academic
Year
Title and
Course
Number
Presentation
Title
2005
BCHM 801
Medical
Biochemistry
2006
BCHM 802
Medical
Biochemistry
2006 present
Foundations
of Medicine
Medical
Biochemistry
2006 present
Genetics &
Neoplasia
Medical
Biochemistry
Clinical:
Instruction
Academic
Year
Type (e.g.
clerkship,
rounding,
clinic, daily
supervision,
teaching)
199
Hours
Type (e.g.
small
group,
laboratory,
seminar,
workshop,
journal
club)
Learner
Type of
Learner(s)
(e.g.
medical
students,
graduate
students,
residents)
Hours
(actual
instruction)
Number
of
Learners
Small
group
discussions
8
7
Medical
Students
Small
group
discussions
10
7
Medical
Students
Small
group
discussions
16
10
Medical
Students
Small
group
discussions
4
10
Medical
Students
Learner
Number of
Learners
Type of
Learner
(e.g.
medical
students,
residents,
fellows)
Length of
Service (e.g.
8 weeks x 6
clerkships;
one month
per resident;
40
hours/week x
6 weeks)
Master’s Theses and PhD Dissertations Directed: List only those for which you
are/were primarily responsible.
Degree
Year
2010 –
present
2012 –
present
Student Name
(completed/in
progress)
Thesis Title
Mauricio VargasUribe
TBD
In progress
TBD
Cassandra Field
In progress
Supervision of Postdoctoral Fellows: List only those for which you are/were
primary supervisor.
Year
Fellow Name
Area of Study
2004 - 2006
Sergiy S. Palchevckyy
Biochemistry
2005 - 2006
Somes K. Das
Biochemistry
2004 - 2008
2006 - present
2007 - present
2012 - present
Yevgen O. Posokhov
Mykola V. Rodnin
Biochemistry
Alexander Kyrychenko
Chiranjib Ghatak
Biochemistry
Biochemistry
Biochemistry
Advising: Thesis or dissertation committees; student academic group/individual
Date
Student or Group Name
Type of Student/Group
2006 - 2012
Hiroo Katayama
PhD thesis committee
2009 –
present
Daniel Parente
Ph.D. thesis committee (SwintKruse lab)
2005 – 2011
Ozan Kumru
Ph.D. thesis committee
Subhaschandra Naik
Damien Pechak, Anna Thoma &
Joshua Brettmann
Ph.D. thesis committee (M.
Fisher lab)
Summer students from William
Jewell College
Other Teaching Activities: CME and Faculty Department Seminars
Date
Title of
Presentation
200
Location
Teaching
Function
Type of
Learner
2. Development of Educational Materials:
Describe any process of peer-review or expert assessment to which materials have
been subjected. Specifically document if the materials have been used or referenced
by colleagues, especially at other institutions or otherwise disseminated. Examples
of course materials include syllabi, educational software packages, web sites, films,
educational tapes, and evaluation tools.
Year
Title Description
2006 - present
Properties of Amino Acids
(eLearning web-based
activity)
Intended Audience
Medical Students
3. Educational Leadership:
Describe administrative responsibility for courses and other leadership activities,
including formal mentoring of junior faculty. Please list faculty members mentored
and your role as mentor- letters from mentorees may be included.
III.
I am a Course Director for BCHM 808 “Methods for Analyzing Biomolecules”
and BCHM 862 “Literature Seminar”
SERVICE ACTIVITIES: Instructions available online.
See Guidelines for Promotion and Tenure, available online, for definitions and suggested
documentation of professional and academic service.
1. Professional Service (Patient Care):
The diverse area of Professional Service includes patient care. Select measures that
most clearly and concisely document your accomplishments and the value of these
activities to the School of Medicine and the University. Measure of both quantity
and quality of activities are required. If necessary, applicants should provide brief
descriptions to assist reviewers. Measures of patient care activities include: number
of patients, time allocation in clinical activity, procedures completed, Relative Value
Units (RVUs), and value to the School of clinical service. If the primary quality
evaluation is the subjective assessment of peers, this should be demonstrated in
letters from departmental colleagues, chair, or referees.
The significance of professional service in the forms of task forces, committees (e.g.
University of Kansas Hospital and University of Kansas Physicians) and similar
groups should be explained and your specific role clarified.
Professional consulting services must have academic credibility and clear service
intent and not be performed primarily for personal profit.
201
2. Academic Service:
In Academic Service, your contribution to the academic community should be
clearly documented. Names and dates of committees, task forces, or working
groups should be provided. A brief description of the significance of the group and
explanation your role should be provided.
Activities related to University of Kansas School of Medicine Academic Societies
should be documented in this section.
Editorial Board Member:
Journal of Membrane Biology
Biopolymers and Cell Journal, Ukraine
External grant reviewer for:
Centre National de la Recherche Scientifique, CNRS, France
Agence Nationale de la Recherche, ANR, France
Natural Sciences and Engineering Research Council of Canada
Reviewer for the following journals:
Biochemistry, Biophysical Journal, JMB, BBA, Biopolymers and Cell, Analytical
Biochemistry, PNAS,
Nature Communications, JBC, JACS, Langmuir, Physical Chemistry, J. of
Pharmacology
Reviewed advanced chapters of the book “Structural Principles of Membrane
Protein Function by Englemann, White and von Hanije for Garland Science
Publishing
Reviewer for the KUMC Biomedical Research Training Program
In charge of Departmental Research Retreat since 2006
Organizer of Telluride Research Workshop “Membrane Protein Folding and
Functioning”, Telluride CO, August 5-9. 2013
Co-Chair of Platform Sessions on Protein-Lipid Interactions at Biophysical Society
Meetings in New Orleans, LA, 2000, Baltimore, MD 2007, and San Francisco, CA,
2010
Co-Chair of Platform Sessions on Membrane Proteins at Biophysical Society
Meetings in Salt Lake City, UT, 2006 and Long Beach, CA 2008
202
Session Chair and Discussion Leader for Lipid and Lipid Protein Interactions,
Gordon Conference on Protons and Membrane Reactions, Ventura, CA, 2012.
Session Chair, American Chemical Society Symposium on “Interfacially Actrive
Peptides, New Orleans, LA, 2013.
IV.
RESEARCH AND SCHOLARLY ACTIVITIES: Instructions available online.
Opening Statement:
Provide a brief opening statement to orient the reviewers to the principal areas of
scholarly activity and provide your perspective. A description of your most
significant contributions to the literature is often helpful to the reviewers.
My research is devoted to understanding of physical principles of proteinmembrane interactions and of the nature of conformational switching during
physiological functioning, such as cellular entry by bacterial toxins and host-defence
peptides, and lately, the regulation of apoptosis.
The highlights of my
accomplishments are devided into the following three categories:
A. Protein-Membrane Interactions: Physical Principles.
My past and present research has been aimed at deciphering the physical principles
that determine the structure, assembly and functioning of membrane proteins.
Specifically, we explored the formative interactions of membrane interfaces on
protein structure. Our main accomplishments are: (1) quantitative characterization
of the interplay of electrostatic and hydrophobic interactions on membrane
interfaces, (2) quantitative characterization of the energetics of secondary structure
formation on membranes, (3) introduction and development of the interfacedirected membrane insertion concept for non-constitutive proteins, (4)
determination of mechanism of action of antimicrobial and other host-defense
peptides. Recently, in collaboration with Jean-Luc Popot (CNRS, Paris, France), we
have developed a number of approaches for measurements of membrane protein
folding and stability, based on application of novel fluorinated surfactants and
amphipols. By combining FCS-based partitioning measurements and a novel
protocol for chaperoned insertion, we have determined the free energy for bilayer
insertion of bacterial toxin and that of a single transmembrane helix and calculated
the free energy of transfer of helical backbone into the hydrocarbon core of the lipid
bilayer. These are the first thermodynamically sound measurement of this kind
which establish a thermodynamic scale of the molecular processes involved in
membrane insertion/refolding transitions.
B. pH-Triggered Conformational Switching and Membrane Insertion of
Diphtheria Toxin T-Domain.
In the past several years the works coming from our lab had made a breakthrough
in understanding the mechanism of conformational switching between soluble and
membrane-inserted forms of Diphtheria Toxin T-Domain (DTT) achived by
203
protonation. We have identified several insertion/refolding intermediates of DTT
and determined free energy differences and transition rates for the transitions along
the refolding/insertion pathway. We have identified a critical histidine residue,
H257, involved in conformational switching of the T-domain in solution and
demonstrated that C-terminal histidines (especially 322) are critical for the later
stages of translocation. Our most recent work combines experiment and molecular
dynamics simulations to reveal the molecular mechanism behind the
conformational switch in DTT. The importance of these findings are not limited to
the field of bacterial action, but is also related to our understanding of similar
proteins, such as those of the BCL-2 family, regulating apoptosis. The initial
characterization of conformational switching during membrane insertion of the prosurvival BCL-XL protein is on currently on the way in my lab.
C. Development of Spectroscopic Methods for Studies of Membrane Proteins.
Over the years we have developed of a series of spectroscopic techniques for
following structural dynamics of macromolecules and for structural and
thermodynamic studies of membrane proteins and peptides. We have developed or
advanced the following spectroscopic techniques utilized to obtain the results
presented above: distribution analysis methodology for depth-dependent
fluorescence quenching analysis of membrane penetration; the lifetime-based
membrane topology method, quenching-enhanced titration method for membrane
binding measurements; lifetime/steady-state FRET protocol for protein
oligomerization on membranes; fluorescence lifetime quenching with transition ion
metals as short-range conformational probes; chaperoned insertion protocol by
fluorinated surfactants for thermodynamic analysis of stability and insertion and an
FCS-based protocol for thermodynamic analysis of pH-dependent membrane
interactions. Our lab has also participated in the development of a highperformance time-resolved fluorescence spectrometer. Currently we are developing
a concerted approach for structural characterization of membrane proteins using
fluorescence spectroscopy and Molecular Dynamics simulations.
1. Grants and Contracts:
Information must include whether you are the principal investigator or a coinvestigator, names of all investigators, title of grant, funding source, dollar amount
in direct costs, and years during which grant applies. Co-investigators must specify
role. Provide the cover sheet, abstract, and Notice of Award in PDF for all grants or
contracts awarded in last five years.
Previous Grants and Contracts Awarded: List in chronological order; oldest
first, newest last; list the applicant’s name in BOLD.
Principal
Title of
Funding
Direct
Investigator Investigators Grant/Contract
Source
Costs
204
Inclusive
Years of
Status
Award
Narayan
Ladokin, A.
Ladokhin
Ladokhin
Effect of
NIH/NCRRR 100,000
Hemifluorinated
Surfactants on
Membrane
Insertion/Folding
of Diphtheria
Toxin T-Domain
20042006
5RO1GM069783
Competing
supplement to:
pH-Triggered
Membrane
Insertion of
Proteins
NIH
$422,228 20072009
Completed
NIH
$62,699
20092010
Completed
KUMC Research
Institute bridging
grant
KUMC
$30,000
20102011
Completed
3RO1GM06978305S1 ARRA
administrative
supplement to:
pH-Triggered
Membrane
Insertion of
Proteins
Ladokhin
Current Grants and Contracts Awarded: List in chronological order; oldest
first, newest last; list the applicant’s name in BOLD.
Inclusive
Principal
Title of
Funding Direct
Years of
Investigator Investigators Grant/Contract Source
Costs
Award
Ladokhin
Tobias,
Kurnikova,
Cocco
pH-Triggered
Membrane
Insertion of
Proteins
NIH
988,000 20112015
Grants and Contracts Submitted: List in chronological order; oldest first, newest
last; list the applicant’s name in BOLD.
Investigators
Principal
Title of
Funding
Direct
Inclusive
205
Comment
[AL1]: Wendy, I need your help
Completed
completing this column.
Status
Funded
Status
Investigator
Goldberg
Grant/Contract
Ladokhin,
Ritter,
Khattree,
Dang
Molecular
scaffolding in
photoreceptor
renewal and
retinal disease
Source
NIH
Costs
Years of
Award
1,250,000 2013 –
2018
2. Scholarly Publications:
Publications must be numbered in chronological order, oldest publication first, most
recent publications last. In cases where there are multiple publications within a
year, they should be listed in alphabetical order.
Articles published: Include full-length, peer-reviewed articles in scientific
journals; this list should not include papers in preparation, submitted, or under
revisions, nor should it include conference proceedings, published abstracts, and
book reviews (which are listed separately). Provide names of all authors
(applicant’s name in BOLD), year, title, journal, volume, and inclusive pages.
Provide a PDF of each peer-review article published within the last five years.
Other articles may be provided at the applicant’s discretion.
1. Demchenko, A. P., Ladokhin, A. S., Kostrzevska, E. G. & Dibrova, T. L.,
1987, Structural dynamics in the environment of the tryptophan residue
in melittin. Molecular Biology 21, 553 560.
2. Kamalov, V. F., Ladokhin, A. S. & Toleutaev, B. N., 1987, Intramolecular
nanosecond dynamics of melittin. Proc. Acad. Sci. USSR 296, 742 745.
3. Demchenko, A. P. & Ladokhin, A. S., 1988, Red-edge excitation
fluorescence spectroscopy of indole and tryptophan. Eur. Biophys. J. 15,
369 379.
4. Demchenko A. P. & Ladokhin, A. S., 1988, Temperature-dependent shift
of fluorescence spectra without conformational changes in protein:
Studies of dipole relaxation in the melittin molecule. Biochim. et Biophys.
Acta. 955, 352 360
5. Ladokhin, A.S., Kostrzevska, E.G. & Demchenko, A.P., 1988, Interaction
of melittin with phospholipid bilayer, Proc. Acad. Sci Ukraine, 11(C), 6558
6. Hershman, P., Ladokhin, A.S., Lebedeva, N.V. & Chikishev, A. Yu, 1989,
Nanosecond dynamics of protein: Time-resolved fluorescent
spectroscopy. Bull. Moscow State University, Ser. 3 30, 47-52.
7. Ladokhin, A. S., Lebedeva, N.V. & Chikishev, A. Uy., 1989, Study of
melittin membrane complex by time-resolved fluorescent spectroscopy.
Biopolymers and Cell, 5, 100-102.
206
Pending
Review
8. Chikishev, A. Yu., Ladokhin, A.S., Lebedeva, N.B. & Toleutaev, B.N., 1990,
Picosecond fluorescence spectrochronography of protein melittin and
melittin-membrane complexes. J. of Molecular Structure, 291, 347-352.
9. Ladokhin, A.S., 1990, Equilibrium dynamics in protein. Melittin intrinsic
fluorescence study. Biopolymers and Cell, 6, 84-90.
10. Ladokhin, A. S., Wang, L., Steggles, A. W. & Holloway, P. W., 1991,
Fluorescence study of a mutant cytochrome b5 with a single tryptophan
in the membrane binding domain. Biochemistry 30, 10200–10206.
11. Ladokhin, A. S., Holloway, P. W. & Kostrzhevska, E. G., 1993, Distribution
analysis of membrane penetration by depth-dependent fluorescence
quenching. J. of Fluorescence 3, 195–197.
12. Ladokhin, A. S., Wang, L., Steggles, A. W., Malak, H. & Holloway, P. W.,
1993, Fluorescence study of a temperature induced conversion from the
“loose” to the “tight” binding form of membrane-bound cytochrome b5.
Biochemistry 32, 6951–6956.
13. Tretyachenko-Ladokhina, V. G., Ladokhin, A. S., Wang, L., Steggles, A. W.
& Holloway, P. W., 1993, Amino acid substitutions in the membranebinding domain of cytochrome b5 alter its membrane-binding
properties. Biochim. et Biophys. Acta 1153, 163–169.
14. Ladokhin, A. S. & Brand, L., 1995, Evidence for an excited-state reaction
contributing to NADH fluorescence. J. of Fluorescence 5, 99–106.
15. Ladokhin, A. S. & Holloway, P. W., 1995, Fluorescence of membranebound tryptophan octyl ester. A model for studying intrinsic
fluorescence of protein–membrane interactions. Biophys. J. 69, 506–517.
16. Ladokhin, A. S. & Holloway, P. W., 1995, Fluorescence quenching study
of melittin-membrane interactions. Ukrainian Biochem. J. 67, 34–40.
17. Ladokhin, A. S., Wimley, W. C. & White, S. H., 1995, Leakage of
membrane vesicle contents: Determination of mechanism using
fluorescence requenching. Biophys. J. 69, 1964–1971.
18. Ladokhin, A. S., Selsted, M. E. & White, S. H., 1997, Bilayer interactions
of indolicidin, a small antimicrobial peptide rich in tryptophan, proline,
and basic amino acids. Biophys. J. 72, 794–805.
19. Ladokhin, A. S., Selsted, M. E. & White, S. H., 1997, Sizing membrane
pores in lipid vesicles by leakage of co-encapsulated markers: Pore
formation by melittin. Biophys. J. 72, 1762–1766.
20. Petrushenko, Z. M., Negrutskii, B. S., Ladokhin, A. S., Budkevich, T. V.,
Shalak, V. F. & El’skaya, A. V., 1997, Evidence for the formation of an
unusual ternary complex of rabbit liver EF-1
 w ith GDP and deacylated
tRNA. FEBS Letters 407, 13–17.
21. Wimley, W. C., Hristova, K., Ladokhin, A. S., Silvestro, L., Axelsen, P. H. &
White, S. H., 1998, Folding of 
-sheet membrane proteins: A
hydrophobic hexapeptide model. J. Mol. Biol. 277, 1091–1110 .
207
22. Ladokhin, A. S., 1999, Analysis of protein and peptide penetration into
membranes by depth-dependent fluorescence quenching: Theoretical
considerations. Biophys. J. 76, 946–955.
23. Ladokhin, A. S., 1999, Evaluation of lipid exposure of tryptophan
residues in membrane peptides and proteins. Analytical Biochem. 276,
65–71.
24. Ladokhin, A. S., 1999, Red-edge excitation study of non-exponential
fluorescence decay of indole in solution and in a protein. J. of
Fluorescence 9, 1–10.
25. Ladokhin, A. S., 1999, Selsted, M. E. & White, S. H. CD spectra of
indolicidin antimicrobial peptides suggest turns, not polyproline helix.
Biochemistry 38, 12313–12319.
26. Ladokhin, A. S. & White, S. H., 1999, Folding of amphipathic 
-helices
on membranes: Energetics of helix formation by melittin. J. Mol. Biol.
285, 1363–1369.
27. Ladokhin, A. S., Jayasinghe, S. & White, S. H., 2000, How to measure and
analyze tryptophan fluorescence in membranes properly, and why
bother? Analytical Biochem. 285, 235–245.
28. Osapay, K., Tran, D., Ladokhin, A. S., White, S. H., Henschen, A. H. &
Selsted, M. E., 2000, Formation and characterization of a single Trp-Trp
crosslink in indolicidin that confers protease stability without altering
antimicrobial activity. J. Biol. Chem. 275, 12017–12022.
29. Ladokhin, A. S., 2001, On the interpretation of decay-associated
fluorescence spectra in proteins. Biopolymers & Cell 17, 221–224.
30. Ladokhin, A. S. & White, S. H., 2001, Alphas and taus of tryptophan
fluorescence in membranes. Biophys. J. 81, 99055–99058.
31. Ladokhin, A. S. & White, S. H., 2001, ‘Detergent-like’ permeabilization of
anionic lipid vesicles by melittin. Biochim. et Biophys. Acta, 1514, 253–
260.
32. Ladokhin, A. S. & White, S. H., 2001, Protein chemistry at membrane
interfaces: Non-additivity of electrostatic and hydrophobic interactions.
J. Mol. Biol. 309, 543–552.
33. Ladokhin, A. S., Isas, J. M., Haigler, H. T. & White, S. H., 2002,
Determining the membrane topology of proteins: Insertion pathway of a
transmembrane helix of annexin 12. Biochemistry 41, 13617-13626.
34. Ladokhin, A. S., Legmann, R., Collier, R. J. & White, S. H., 2004,
Reversible refolding of the diphtheria toxin T-domain on lipid
membranes. Biochemistry 43, 7451-7458.
35. Ladokhin, A. S. & White, S. H., 2004, Interfacial folding and membrane
insertion of a designed helical peptide. Biochemistry 43, 5782-5791.
36. Ladokhin, A. S. and Haigler, H. T., 2005, Reversible Transition between
the Surface Trimer and Membrane-Inserted Monomer of Annexin 12.
208
37. Patel, D. R., Isas, J. M., Ladokhin, A. S., Jao, C. C., Kim, Y. E., Kirsch, T.,
Langen. R. and Haigler, H. T., 2005, The Conserved Core Domains of
Annexins A1, A2, A5, and B12 Can Be Divided into Two Groups with
Different Ca2+ -Dependent Membrane-Binding Properties. Biochemistry
44, 2833-2844.
38. Palchevskyy, S.S, Posokhov, Y.O., Olivier, B., Popot, J-L, Pucci, B. and
Ladokhin, A. S., 2006 Chaperoning of insertion of membrane proteins
into lipid bilayers by hemifluorinated surfactants: application to
diphtheria toxin Biochemistry 45, 2629-2635.
39. Posokhov, Y.O. and Ladokhin, A. S., 2006, Lifetime Fluorescence Method
for Determining Membrane Topology of Proteins, Analytical Biochem.
348, 87-93.
40. Fernandez-Vidal M., Jayasinghe S., Ladokhin A.S., White S.H., 2007,
Folding Amphipathic Helices Into Membranes: Amphiphilicity Trumps
Hydrophobicity, J. Mol. Biol. 370(3):459-70.
41. Posokhov, Y.O., Gottlieb, P.A. and Ladokhin, A. S., 2007, Quenchingenhanced fluorescence titration protocol for accurate determination of
free energy of membrane binding, Analytical Biochem. 362, 290-292.
42. Posokhov, Y.O., Gottlieb, P.A., Morales, M.J., Sachs, F. and Ladokhin, A. S.
,2007, Is Lipid Bilayer Binding a Common Property of Inhibitor Cysteine
Knot Ion-Channel Blockers? Biophysical Journal 93(4):L20-2.
43. Ladokhin, A. S., 2008, Insertion Intermediate of Annexin B12 is Prone to
Aggregation on Membrane Interfaces. Biopolymers and Cell, 24(2):100104.
44. Posokhov, Y.O., Merzlyakov, M., Hristova, K. and Ladokhin, A. S., 2008, A
simple "proximity" correction for Förster resonance energy transfer
efficiency determination in membranes using lifetime measurements,
Analytical Biochem., 380, 134-136.
45. Posokhov, Y.O., Rodnin M.V., Das S.K., Pucci B., Ladokhin A.S., 2008, FCS
study of the thermodynamics of membrane protein insertion into the
lipid bilayer chaperoned by fluorinated surfactants. Biophysical Journal,
95, L54-6..
46. Posokhov, Y.O., Rodnin, M.V., Lu, L. and Ladokhin, A. S., 2008, Membrane
Insertion Pathway of Annexin B12: Thermodynamic and Kinetic
Characterization by FCS and Fluorescence Quenching. Biochemistry 47,
5078-87.
47. Rodnin, M.V., Posokhov, Y.O., Contino-Pepin, C., Brettmann, J.,
Kyrychenko, A., Palchevskyy, S.S.. Pucci, B., and Ladokhin, A. S., 2008,
Interactions of fluorinated surfactants with diphtheria toxin T-domain:
Testing new media for studies of membrane proteins. Biophysical
Journal, 94, 4348-57.
48. Kyrychenko, A., Posokhov, Y.O., Rodnin M.V., Ladokhin A.S., 2009,
Kinetic intermediate reveals staggered pH-dependent transitions along
209
49.
50.
51.
52.
53.
54.
55.
56.
57.
58.
59.
60.
the membrane insertion pathway of the diphtheria toxin T-domain.
Kyrychenko A., Wu F., Thummel R.P., Waluk J., Ladokhin A.S., 2010,
Partitioning and Localization of Environment-Sensitive 2-(2′-Pyridyl)and 2-(2′-Pyrimidyl)-Indoles in Lipid Membranes: A Joint Refinement
Using Fluorescence Measurements and Molecular Dynamics Simulations
J. Phys. Chem. B 114, 13574-13584.
Ladokhin A.S., Fernandez-Vidal M., White S.H., 2010, CD Spectroscopy of
Peptides and Proteins Bound to Large Unilamellar Vesicles, J. Membrane
Biol. 236, 247-253.
Muretta J.M., Kyrychenko A., Ladokhin A.S., Kast D.J., Gillispie G.D., and
Thomas D.D., 2010, High-performance time-resolved fluorescence by
direct waveform recording, Rev. Sci. Instrum. 81, 103101.
Posokhov, Y.O., Kyrychenko, A., Ladokhin A.S., 2010, Steady-state and
time-resolved fluorescence quenching with transition metal ions as
short-distance probes for protein conformation. Analytical Biochem.,
407, 284-286.
Rodnin M.V., Kyrychenko A., Kienker P., Sharma O., Posokhov Y.O., Collier
R.J., Finkelstein A., Ladokhin A.S., 2010, Conformational Switching of the
Diphtheria Toxin T Domain. J. Mol. Biol, 402, 1-7.
Fernandez-Vidal M., White S.H., Ladokhin A.S., 2011, Membrane
partitioning: "classical" and "nonclassical" hydrophobic effects. J.
Membrane Biol., 239, 5-14.
Kyrychenko A., Sevriukov I.Y, Syzova Z.A, Ladokhin A.S., 2011,
Doroshenko A.O. Partitioning of 2,6-Bis(1H-Benzimidazol-2-yl)pyridine
fluorophore into a phospholipid bilayer: Complementary use of
fluorescence quenching studies and molecular dynamics simulations.
Biophys. Chem, 154, 8-17.
Kumru OS, Schulze RJ, Rodnin MV, Ladokhin A.S., Zückert W.R., 2011,
Surface localization determinants of Borrelia OspC/Vsp family
lipoproteins J. Bacteriol. 193:2814-25.
Rodnin M.V., Kyrychenko A., Kienker P., Sharma O., Vargas-Uribe M.,
Collier R.J., Finkelstein A., Ladokhin A.S. Replacement of C-Terminal
Histidines Uncouples Membrane Insertion and Translocation in
Diphtheria Toxin T-Domain. Biophysical Journal, 101:L41-L43.
Almeida P., Ladokhin A.S., 2012, White S.H. Hydrogen-bond energetics
drive helix formation in membrane interfaces. BBA, Special Issue
“Membrane protein structure and function”, 1818:178-182.
Kyrychenko A., Rodnin M.V., Posokhov Y.O., Halt A., Pucci B., Killian J.A.,
Ladokhin A.S., 2012, Thermodynamic measurements of bilayer
insertion of a single transmembrane helix chaperonned by fluorinated
surfactants. J. Mol. Biol, 416:328-334.
Kyrychenko A., Rodnin M.V., Vargas-Uribe M., Sharma S.K., Durand G.,
Pucci B., Popot J.-L., Ladokhin A.S., 2012, Folding of diphtheria toxin T210
domain in the presence of amphipols and fluorinated surfactants:
Toward Thermodynamic measurements of membrane protein folding.
BBA, Special Issue “Protein folding in membranes”, 1818:1006-12.
61. Kyrychenko A., Tobias D.J., Ladokhin A.S., 2013, Validation of DepthDependent Fluorescence Quenching in Membranes by Molecular
Dynamics Simulation of Tryptophan Octyl Ester in POPC Bilayer. J. Phys.
Chem. B., 117, 4770-8.
Manuscripts in press: Provide names of all authors (applicant’s name in
BOLD), title, journal, and PDF of manuscript, plus evidence of acceptance by
journal.
1. Kurnikov I., Kyrychenko A., Flores-Canales J., Rodnin M.V., Simakov N.,
Vargas-Uribe M., Posokhov Y.O., Kurnikova M., Ladokhin A.S. pHTriggered conformational switching of the diphtheria toxin T-domain: The
roles of N-terminal histidines. JMB.
2. Kyrychenko A., Ladokhin A.S. Molecular Dynamics Simulation of Depth
Distribution of Spin-Labeled Phospholipids within Lipid Bilayer. J. Phys.
Chem. B.
3. Vargas-Uribe M., Rodnin M.V., Kienker P., Finkelstein A., Ladokhin A.S.
Crucial Role of H322 in the Folding of Diphtheria Toxin T-Domain into the
Open-Channel State. Biochemistry.
Manuscripts submitted by not yet accepted: Provide names of all authors
(applicant’s name in BOLD), title, journal, and PDF of manuscript, plus evidence
of acceptance by journal.
1.
Invited or non-peer –reviewed articles or reviews: Provide names of all
authors (applicant’s name in BOLD), year, title, journal, volume, and pages. If in
press, provide documentation and PDF of article/review if published within the
last five years.
1. Ladokhin, A. S., 1997, Distribution analysis of depth-dependent
fluorescence quenching in membranes: A practical guide. Methods in
Enzymology 278, 462–473.
2. Ladokhin, A. S., Wimley, W. C., Hristova, K., & White, S. H., 1997,
Mechanism of leakage of contents of membrane vesicles determined by
fluorescence requenching. Methods in Enzymology 278, 474–486.
3. White, S. H., Wimley, W. C., Ladokhin, A. S, & Hristova, K., 1998,
Methods for determining the energetics of peptide–bilayer interactions.
Methods in Enzymology 295, 62–87.
211
4. White, S. H., Wimley, W. C., Ladokhin, A. S, & Hristova, K., 1998, Protein
folding in membranes: Pondering the nature of the bilayer milieu. Biol.
Skr. Dan. Selsk. 49, 99–106.
5. White, S. H., Ladokhin, A. S, Jayasinghe, S. & Hristova, K., 2001, How
membranes shape protein structure. J. Biol. Chem. 276, 32395–32398.
6. Ladokhin, A. S., 2009, Fluorescence spectroscopy in thermodynamic
and kinetic analysis of pH-dependent membrane protein insertion.
Methods in Enzymology 466, 19-42.
Books and book chapters: Provide names of all authors (applicant’s name in
BOLD), year, book title, chapter title, edition, publisher, and pages. If in press,
provide documentation and PDF of book/book chapter if published in last five
years.
1. Ladokhin, A. S., 2000, Encyclopedia of Analytical Chemistry:
Instrumentation and Applications, “Fluorescence spectroscopy in
peptide and protein analysis”, 15 volume set editions, Wiley, 5762-5779.
2. London, E. & Ladokhin, A. S., 2002, Current Topics in Membranes:
Peptide–Lipid Interactions, “Measuring the depth of amino acid residues
in membrane-inserted peptides by fluorescence quenching”, 1 edition,
Academic Press, 89-115.
Published Abstracts: Provide names of all authors (applicant’s name in BOLD),
year, title, where published, volume and pages. If the same work is reflected in a
published abstract and a presentation or poster, the work must only be listed once.
1.
Other Scholarly Publications:
1.
3. Presentations and Posters:
Presentations and posters must be number in chronological order, oldest first, most
recent last.
Oral paper presentation:
Provide names of all authors (applicant’s name in BOLD), title, sponsoring
organization, extent of peer-review, and location and date of presentation.
212
1. Ladokhin, A.S., ‘Fluorescence approaches for studying membrane
structure and dynamics.’ Biophysical Society Meeting, Baltimore, MD,
1999. Fluorescence Subgroup Panel Discussion
2. Ladokhin, A.S., Biophysical Society Meeting, New Orleans, LA (2000).
Co-Chair of Platform Session on Protein–Lipid Interactions.
3. Ladokhin, A.S., “Reversible Refolding of the Diphtheria Toxin T-domain
on Membranes”ASBMB Meeting, San Diego, CA, 2003
4. Ladokhin, A.S., Gordon Conference on Antimicrobial Peptides, Ventura,
CA, 2005.
5. Ladokhin, A.S., American Chemical Society Meeting, San-Francisco, CA,
2006. , speaker
6. Ladokhin, A.S., Biophysical Society Meeting, Salt Lake City, UA, 2006.
Co-Chair of Platform Session on Membrane Proteins.
7. Ladokhin, A.S., American Chemical Society Meeting, Atlanta, GA, 2006,
speaker
8. Ladokhin, A.S., “pH-triggered membrane protein folding and insertion:
Annexin B12 as a model” VI International Annexin Conference, Avalon,
CA, 2007. Invited Speaker
9. Ladokhin, A.S., “Using novel fluorinated surfactants to chaperone
membrane protein insertion” NIH/NIAAA/Lab. Mem. Biochem &
Biophys., Bethesda, MD, 2007. (Lecture)
10. Ladokhin, A.S., Biophysical Society Meeting, Baltimore, MD, 2007. CoChair of Platform Session on Lipid-Protein Interactions.
11. Ladokhin, A.S., Biophysical Society Meeting, Long Beach, CA, 2008. CoChair of Platform Session on Membrane Proteins.
12. Ladokhin, A.S., 23rd Annual Gibbs Conference in Biothermodynamics,
Carbondale, IL, 2009, invited speaker
13. Ladokhin, A.S., Gordon Conference on Biophysics of Membrane
Transport, Waterville, ME, 2009, invited speaker
14. Ladokhin, A.S., Workshop on Biophysics of Ion Channels, Telluride, CO,
2009, invited speaker
15. Ladokhin, A.S., “Frontiers in Membrane and Membrane Protein
Biophysics: Experiment and Theory” Symposium, Irvine, CA, 2010,
speaker
16. Ladokhin, A.S., FASEB Meeting on Biophysics of Cellular Membrane,
Saxtons River, Vermont 2010. Oral presentation selected from the
abstracts
17. Ladokhin, A.S., Biophysical Society Meeting, San Francisco, CA, 2010.
Co-Chair of Platform Session on Lipid-Protein Interactions.
18. Ladokhin, A.S., Biophysical Society Meeting, Baltimore, MD, 2011.
Membrane Structure and Assembly Subgroup Symposium, Invited
Speaker.
213
19. Ladokhin, A.S., “Conformational switching during pH-triggered
membrane protein insertion” FASEB Meeting on Biophysics of Cellular
Membrane, Snowmass, CO, 2012
20. Ladokhin, A.S., Gordon Conference on Protons and Membrane
Reactions, Ventura, CA, 2012, Session Chair and Discussion Leader for
Lipid and Lipid Protein Interactions
21. Ladokhin, A.S., Membrane Protein Folding Symposium of the
Biophysical Society, Seoul, South Korea, 2013
22. Ladokhin, A.S., “Interfacially Active Peptides”, American Chemical
Society Symposium on New Orleans, LA 2013, Session Chair and invited
speaker
1.
Poster Presentations:
Provide names of all authors (applicant’s name in BOLD), title, sponsoring
organization, extent of peer-review, and location and date of presentation.
Poster presentation at national and international meetings:
1.
Poster presentation at local and regional meetings:
1.
Invited seminars at other universities:
Provide title, sponsoring organization or institution, and date of presentation.
1. “Excited-state reactions and NADH fluorescence”, Agricultural
University, Wageningen, The Netherlands, 1993
2. “Leakage of membrane vesicle contents: Determination of mechanism
using fluorescence requenching”, Beckman Laser Institute, Irvine, CA,
1995
3. “Membrane permeabilization: Pieces of the puzzle”, Rutgers Univ., New
Brunswick, NJ, 1998
4. “Membrane protein folding: Thermodynamic and kinetic control”,
Institute of Molecular Biology and Genetics, Kiev, Ukraine, 2001
5. Lecture, SNRS, Institut de Biologie Physico-Chimique, Paris, 2005
6. “Membrane Protein Folding: from Basic Principles to Insertion
Pathways”, UMKC, Kansas City, MO, 2005
214
7. “Membrane Protein Folding: from Basic Principles to Insertion
Pathways”, University of Aalborg, Department of Life Sciences, Aalborg,
Denmark, 2006
8. “Membrane Protein Folding: from Basic Principles to Chaperoned
Insertion”, Tulane University, New Orleans, LA, 2007
9. “Why study single biomolecules?”, Institute of Molecular Biology and
Genetics, Kiev, Ukraine, 2007
10. “pH-Triggered Membrane Protein Insertion: Thermodynamic and
Kinetic Aspects”, Carnegie Mellon University, Pittsburgh, PA, 2009
11. “Thermodynamics of pH-Triggered Membrane Protein Insertion: what
can we learn about membrane protein stability?” Boston University,
Boston, MA, 2009
12. “Kinetic and thermodynamic studies of membrane insertion of
diphtheris toxin T-domain “, SNRS, Institut de Biologie PhysicoChimique, Paris, France, 2010
13. “Fluorinated surfactants as chaperons for thermodynamic studies of
membrane proteins“, Université de Avignon, Avignon, France, 2010
14. “Kinetic and thermodynamic studies of membrane insertion of diptheris
toxin T-domain”, Institute of Physical Chemistry, Polish Academy of
Sciences, Warsaw, Poland, 2010
15. “Why study single biomolecules: One lab perspective”, Institute for
Biotechnology, Shevchenko National University, Kiev, Ukraine, 2010
16. “pH-Triggered Conformational Switching and Membrane Insertion of
Diphtheria Toxin T-Domain”, Department of Chemistry, UC-Irvine, 2013
17. “Deciphering pH-Triggered Membrane Protein Insertion with
Experiment and Simulation”, Department of Chemistry, Washington
Univ. St. Louis, 2013
18. “Deciphering pH-Triggered Membrane Protein Insertion with
Experiment and Simulation”, Department of Chemistry, Univ. of
Missouri-Columbia, 2013
4. Other Evidence of Scholarship:
Includes clinical guidelines, policy documents, contributions to significant position
statements by professional organizations, and development of national
examinations.
1.
VII.
VIII.
IX.
215
First Name
Owen
Last name
Nadeau
Research Assistant Professor
Department(s)
Dept. of Biochemistry and Molecular Biology
Office Address
1061 Hemenway Building
Phone
913-588-3486
Fax
913-588-9896
Email
[email protected]
Years (Inclusive)
Degree
Institution
1977 – 1981
B.S., Chemistry
Indiana University of
Pennsylvania, Indiana, PA
1981-1984
M.S., Chemistry
Pennsylvania, Indiana, PA
1984 – 1991
Ph.D., Biochemistry
University of Vermont,
Burlington, VT
Years (Inclusive)
Degree
Institution
1991-1997
Postdoctoral Fellow
(Biochemistry)
University of Tennessee,
Memphis, TN
1997
Postdoctoral Fellow
(Pathology)
Memphis, TN
Month and Year
Position
Institution
1982-1984
Teaching Assistant
Pennsylvania, PA
216
1984-1988
Teaching Fellow (Chemistry)
Burlington, VT
1988-1991
Research Fellow
(Biochemistry
Burlington, VT
1991 – 1997
Postdoctoral Fellow
(Biochemistry and Molecular
Biology)
Memphis, TN
1997-1999
Senior Scientist, Group
Leader
Hexos Inc, Bothell, WA
1999-2002
University of Missouri –
Kansas City
20030- present
Center
Professional Registration/Licensure
Year
Number
State
Date
1989-1995
Sigma Xi
1990- present
Protein Society
2004 – present
American Society for Mass Spectrometry
1995 – present
American Society for Biochemistry and Molecular
Biology
1982 - present
Year
Award
1984
Academic Achievement Award, Indiana
University of Pennsylvania
1984
Graduate Research Award, Indiana University of
Pennsylvania
217
1993-1995
American Heart Associate, Tennessee Affiliate –
Postdoctoral Research Fellowship
au
X. TEACHING ACTIVITIES
(Teaching evaluations and other evidence of quality teaching must be attached; this
represents the teacher’s portfolio and should be accurately summarize ALL of your teaching
activities.)
7. Instruction:
Instruction
Academic
Year
Course
Title
Type
Student
Hours
No
Type
2004
BCHM
850
ProteinProtein
Interactions
Lecture
2009
BCHM
923
Adv. Protein
Structure and
Function
Lecture
2
Graduate
2011
BCHM
923
Adv. Protein
Structure and
Function
Lecture
2
Graduate
Graduate
Instruction
Academic
Year
Course
Title
Type
Hours
Student
No
Type
2006
Foundations
st
Medicine (1
Yr SOM)
Facilitator
Small
Group
Discussions
M1
2007
Foundations
st
Medicine (1
Yr SOM)
Facilitator: Cancer
Screening
Small
Group
Discussions
M1
218
2008
Genetics &
Neoplasia
st
(1 Yr SOM)
Facilitator:
Genetics/Neoplasia
Small
Group
Discussions
M1
2009
Foundations
st
Medicine (1
Yr SOM)
Facilitator: Cancer
Screening
Small
Group
Discussions
M1
2009
Foundations
st
Medicine (1
Yr SOM)
Facilitator:
Huntingtins
Small
Group
Discussions
M1
2010
Genetics &
Neoplasia
st
(1 Yr SOM)
Facilitator:
Genetics/Neoplasia
Small
Group
Discussions
M1
Clinical
Length of
Service
Student
Year
Hours
No
Type
Year
Student Name
Thesis Title
Degree
(Completed/In process)
Year
Fellow Name
Area of Study
Advising (Thesis or dissertation committees: student academic group/individual)
Date
219
Date
Title
Place
Teaching Function
Year
Title Description
Intended Audience
2007
Post-translational modification of Proteins
Web resource for Medical
Curriculum
9. Educational Leadership
(Responsibility for courses and other leadership activities including mentoring of junior
faculty. Please list faculty members mentored – letters from mentorees may be provided.)
SERVICE ACTIVITIES
Professional Service:
The diverse area of professional service includes patient care. Applicants should select
measures that most clearly and concisely document their accomplishments and the value of
these activities to the Medical School and University. Measures of both quantity and quality of
activities are required and if necessary, applicants should provide brief descriptions to assist
reviewers. Measures of patient care activities include numbers of patients, time allocation
in clinical activity, procedures completed, Relative Value Units (RVUs), and value to the
School of the clinical service. If the primary quality evaluation is the subjective assessment of
peers, this should be available in letters from departmental colleagues, chair, or referees.
The significance of professional service in the forms of task forces, committees and similar
groups should be explained and the specific role of the applicant clarified.
Professional consulting services must have academic credibility and clear service intent and not
be performed primarily for personal profit.
Academic Service:
In academic service the contribution of the candidate to the academic community
should be clearly documented. Names and dates of committees, task forces, or working
groups should be provided. A concise description of the significance of the group and
explanation of the role of the applicant should be provided.
Activities related to Academic Societies should be documented in this section.
220
XI. RESEARCH and SCHOLARLY ACTIVITIES:
Our primary goal and continuing efforts are centered on determining how subunit
interactions affect the activation state of phosphorylase kinase (PhK), a 1.3 MDa
hexadecameric complex comprising four copies of four distinct subunits, termed α, β, γ
and δ, which is endogenous calmodulin. In the PhK complex, the catalytic γ subunit is
activated by neural (Ca2+), hormonal (cAMP and Ca2+) and metabolic (ADP) stimuli,
which are integrated through allosteric sites on the regulatory α, β and δ subunits. This
activation of PhK by diverse physiological signals allows for tight control of
glycogenolysis. Activation of PhK by Ca2+ in skeletal muscle directly links muscle
contraction with energy production in the cascade activation of glycogen utilization.
To analyze the subunit interactions of PhK we have developed a data-based approach to
detect protein-protein interactions using a combination of chemical cross-linking and
mass spectrometric (MS) methodologies. Recent developments in MS technologies have
significantly reduced the amount of starting material required to detect specific contact
regions between interacting proteins, leading to a resurgence in classical protein
chemical approaches, including chemical cross-linking. Despite the resolving power of
MS, considerable computational analyses of digests from conjugates of interacting
proteins are required to predict all the possible masses that can arise from
monoderivatization, intramolecular cross-linking, incomplete digestion of the target
proteins, hydrolysis and other side reactions associated with chemical cross-linking.
Considering the complexity of the products formed, we have developed a search engine
that predicts both conjugates and side-products of such reactions. The search engine
automatically eliminates false positives arising from known competing side reactions,
thus allowing for rapid screening of MS peptide maps of protein conjugates for potential
cross-linked peptides, which are then ranked hierarchically by theoretical best match.
Since several combinations of cross-linking can lead to identical mass assignments
within the error limits of state-of-the-art MS technologies, all cross-linked peptides must
be verified by fragmentation (MS/MS) analyses, which in turn generate even more
complex forms of data output that often must be annotated by hand. To eliminate this
‘second’ rate limiting step in the method, we are currently expanding the search engine’s
capability to generate potential fragment ion patterns for those cross-linked peptides that
are predicted in the first round of the analysis. A web-based version of the engine is
under construction, and the initial results can be viewed at
http://funnybase.umkc.edu/prot_cross3/.
Grants and contracts
(Information must include whether the nominee (name bolded) is the principal
investigator or a co-investigator, names of all investigators, title of grant, funding source,
dollar amount in direct costs, and years during which grant applies. Co-investigators
must specify role). Provide the cover sheet, abstract and Notice of Award
in PDF for all grants or contracts awarded in last five years (submit
online).
8.
Previous Grants and contracts awarded:
(List in chronological order – oldest first, newest last)
221
Principal
Investigator
Title of
Grant
Investigators
Funding
Source
Direct
Costs
Years
Status
Direct
Costs
Years
Status
Funding
Source
Direct
Costs
Years
Status
NIH
123,000
1-4
pending
Current Grants and contracts awarded:
(List in chronological order – oldest first, newest last)
Principal
Investigator
Title of
Grant
Investigators
Funding
Source
Principal
Investigator
Investigators
XlinkID: An
Automated Data
Analysis Platform
for Structural
Studies of
Proteins Using
Chemical CrossLinking and
Owen
Nadeau and
Xiuxia Du
9.
Title of Grant
Tandem Mass
Spectrometry
year, title, journal, volume, and inclusive pages. The articles must be numbered in
chronological order (oldest publications first, most recent publications last). In cases
where there are multiple publications within a year, they should be listed by alphabetical
order. The list should not include papers "in preparation," "submitted," or "under
revisions", nor should it include conference proceedings, published abstracts, and book
reviews (which need to be listed separately).
Articles published: (Provide a PDF of each peer-reviewed article published within
1.
Hubbard, J.L., Morneau, A., Burns, R.M. and Nadeau, O.W. (1991) Carbon-carbon
Double Bond Formation from a cis-bis (chloromethyl) Complex, J. Am. Chem. Soc.
113:9180-9184.
2. Nadeau, O.W., Gump, D.W., Hendricks, G.M. and Meyer, D.H. (1992) Deposition of
Bismuth by Yersinia nterocolitica, Med. Microbiol. Immunol. 181:145-152.
222
3. Gump, D.W., Nadeau, O.W., Hendricks, G.M. and Meyer, D.H. (1992)“Evidence that
Bismuth Salts Reduce Invasion of Epithelial Cells by Enteroinvasive Bacteria, Med.
Microbiol. Immunol. 181:131-143.
4. Nadeau, O.W. and Carlson, G.M. (1994) Zero-length Conformation-dependent Crosslinking of Phosphorylase Kinase Subunits by Transglutaminase, J. Biol. Chem.
269:29670-2967.
5. Nadeau, O.W., Falick, A. and Woodworth, R.C. (1996) Structural Evidence For An AnionDirecting Track in the Hen Ovotransferrin N-Lobe: Implications for Transferrin Synergistic
Anion-Binding, Biochemistry 35:14294-14303.
6. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1997)“Differential Affinity Cross-Linking of
Phosphorylase Kinase
Conformers by the Geometric Isomers of Phenylenedimaleimide, J. Biol. Chem.
272:26196-26201.
7. Domanski, P., Fish, E., Nadeau, O.W., Witte, M., Platanias, L.C., Yan, H., Krolewski, J.,
Pitha, P. and Colamonici,
O.R. (1997) A Region of the β Subunit of the Interferon a Receptor Different from Box 1
Interacts With Jak1 and Is Sufficient To Activate the Jak-Stat Pathway and Induce an
Antiviral State, J. Biol. Chem. 272:26388-26393.
8. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1997) The Structural Effects of
Endogenous and Exogenous
2+
Ca /Calmodulin on Phosphorylase Kinase, J. Biol. Chem. 272:26202-26209.
9. Domanski, P., Nadeau, O.W., Platanias, L.C., Fish, E., Kellum, M., Pitha, P. and
Colamonici, O.R. (1998) Differential Use of the β L Subunit of the Type I Interferon
Receptor Determines Signaling Specificity for IFNα2 and IFNβ, J. Biol. Chem. 273:31443147.
10. Platanias, L.C., Domanski, P., Nadeau, O.W., Yi, T., Uddin, S., Fish, E., Neel, B.G. and
Colamonici, O.R. (1998)
Identification of a Domain in the β Subunit of the Type I Interferon (IFN) Receptor that
Exhibits a Negative
Regulatory Effect in the Growth Inhibitory Action of Type I IFNs, J. Biol. Chem. 273:55775581.
11. Ayers, N.A., Nadeau, O.W., Read, M.W., Ray, P. and Carlson, G.M. (1998)“Effectorsensitive Cross-linking of
Phosphorylase-b Kinase by the Novel Cross-linker 4-phenyl-1,2,4-triazoline-3,5-dione,
Biochem. J. 331:137-141.
12. Nadeau, O.W., Traxler, K.W. and Carlson, G.M. (1998) Zero-length Cross-linking of the β
Subunit of Phosphorylase Kinase to the N-terminal Half of its Regulatory α Subunit,
Biochem. Biophys. Res. Commun. 251:637-641.
223
13. Nadeau, O.W., Traxler, K.W., Fee, L.R., Baldwin, B.B. and Carlson, G.M. (1999)
Activators of Phosphorylase
th
Kinase Alter the Cross-linking of its Catalytic Subunit to the C-terminal 1/6 of its
Regulatory α Subunit, Biochemistry 38:2551-2559.
14. Carlson, G.M. and Nadeau, O.W.(1999) Bifunctional Crosslinking Reagents, in The
Encyclopedia of Molecular
Biology, Ed. T.E. Creighton, Wiley, New York, pp 279-28.
15. Carlson, G.M. and Nadeau, O.W.(1999) Crosslinking, in The Encyclopedia of Molecular
Biology, Ed. T.E. Creighton, Wiley, New York, pp 582-584.
16. Carlson, G.M. and Nadeau, O.W. (1999) Enzyme Immobilization and Conjugation, in The
Encyclopedia of Molecular Biology, Ed. T.E. Creighton, Wiley, New York, pp 829.
17. Carlson, G.M. and Nadeau, O.W.(1999) “Gluteraldehyde,” in The Encyclopedia of
Molecular Biology, Ed. T.E.
Creighton, Wiley, New York, pp 1019-1020.
18. Nadeau, O.W., Domanski, P., Usacheva, A., Uddin, S., Platanias, L.C., Pitha, P., Raz, R.,
Levy, D., Majchrzak,
B., Fish, E. and Colamonici, O.R. (1999) The Proximal Tyrosines of the Cytoplasmic
Domain of the β Chain of
the Type I Interferon Receptor are Essential for Signal Transducer and Activator of
Transcription (Stat) 2 Activation, J. Biol. Chem. 274:4045-4052.
2+
19. Nadeau O. W., Carlson, G.M. and Gogol, E.P. (2002) A Ca -Dependent Global
Conformational Change in the
3D Structure of Phosphorylase Kinase Obtained from Electron Microscopy, Structure
10:23-32.
20. Rice, N.A., Nadeau, O.W., Yang, Q. and Carlson, G. M. (2002) The Calmodulin-binding
Domain of the Catalytic
γ Subunit of Phosphorylase Kinase Interacts with Its Inhibitory α Subunit, J. Biol. Chem.
277:14681-14687.
21. Nadeau, O.W. and Carlson, G.M. (2002) Chemical Crosslinking in Studying Proteinprotein Interactions, in
Protein-Protein Interactions, Chap. 6, Ed. Erica Golemis, Cold Spring Harbor Laboratory
Press, New York, pp 75-91 .
22. Marshal, S, Nadeau, O.W. and Yamasaki, K. (2004) Dynamic Actions of Glucose and
Glucosamine on ,
Hexosamine Biosynthesis in Isolated Adipocytes. J. Biol. Chem. 279: 35313-35319.
23. Priddy, T.S., MacDonald. B.A., Heller, W.T., Nadeau, O.W., Trewhella, J., and Gerald M.
2+
Carlson, (2005) Ca 224
induced Structural Changes in Phosphorylase Kinase Detected by Small-angle X-ray
Scattering. Protein Sci 14,
1039-1048.
24. Nadeau, O. W., Gogol E. and Carlson, G. M. (2005) Cryoelectron Microscopy Reveals
New Features in the
Three-dimensional Structure of Phosphorylase Kinase. Protein Sci 14, 914-920.
25. Marshall, S, Nadeau, O and Yamasaki, K, (2005) Glucosamine-induced Activation of
Glycogen Biosynthesis in
Isolated Adipocytes: Evidence for a Rapid Allosteric Control Mechanism within the
Hexoseamine Biosynthesis
Pathway, J. Biol. Chem. 280, 11018-11024.
26. Nadeau, O. W. and Carlson, G. M. (2005) Protein Interactions Captured by Chemical
Cross-linking, in ProteinProtein Interactions, 2nd ed, Chapter 7, Eds. Erica Golemis and Peter Adams, Cold
Spring Harbor Laboratory
Press, New York pp 105-127.
27. Nadeau, O. W. (2006) Protein-protein interaction analysis: chemical cross-linking, in
Encyclopedic Reference
of Genomics and Proteomics in Molecular Medicine, Eds. K. Ruckpaul and D. Ganten,
Springer-Verlag,
Heidelberg, online version at
http://www.springerlink.com/content/n4425664628508x7/fulltext.html.
28. Nadeau, O.W., Anderson, D.W., Yang, Q., Artigues, A., Paschall, J.E., Wyckoff, G.J.,
McClintock J.L., and
Carlson, G. M. (2007) Evidence for the Location of the Allosteric Activation Switch in the
Multisubunit
Phosphorylase Kinase Complex from Mass Spectrometric Identification of Chemically
Cross-linked Peptides.
J. Mol. Biol. 365, 1429-1445.
29. Owen W. Nadeau, Gerald J. Wyckoff, Justin E. Paschall, Antonio Artigues, Jessica
Sage, Maria T. Villar and
Gerald M. Carlson, (2008) Cross Search, a User-friendly Search Engine for Detecting
Chemically Cross-linked
Peptides in Conjugated Proteins, Mol. Cell. Proteomics, 7, 739-749.
30. Igor G. Boulatnikov, Owen W. Nadeau, Patrick J. Daniels, Jessica M.
Sage, Marina D. Jeyasingham, Maria T.
225
Villar, Antonio Artigues, and Gerald M. Carlson, (2008) The Regulatory β
Subunit of Phosphorylase Kinase
Interacts with Glyceraldehyde-3-phosphate Dehydrogenase, Biochemistry 47, 7228
–
7236
31. Marina D. Jeyasingham, Antonio Artigues, Owen W. Nadeau and Gerald M. Carlson,
(2008) Structural Evidence
for Co-Evolution of the Regulation of Contraction and Energy Production in Skeletal
Muscle, J. Mol. Biol. 377,
623-629.
32. Tran, Q-K., Leonard, J., Black, D.J., Nadeau, O.W., Boulatnikov, I.G., and Persechini,
A., (2009) Effects of
combined phosphorylation at Ser-617 and Ser-1179 in endothelial nitric-oxide synthase
2+
on EC 50 (Ca ) values for
calmodulin binding and enzyme activation. J. Biol. Chem. 284, 11982-11899. PMID:
19251696
33. Boulatnikov, I.G., Peters, J.L., Nadeau, O.W., Sage, J.M., Daniels, P.J., Kumar, P.,
Walsh, D.A. and Carlson,
G.M. (2009) Expressed phosphorylase b kinase and its αγδ subcomplex as regulatory
models for the rabbit
skeletal muscle holoenzyme. Biochemistry 48, 10183-10191. PMID: 19764815.
34. Nadeau, O.W., Liu, W., Boulatnikov, I.G., Sage, J.M., Peters, J.L. and Carlson, G.M.
(2010) The glucoamylase
inhibitor acarbose is a direct activator of phosphorylase kinase. Biochemistry 49, 65056507.
Manuscripts in press: (Provide names of all authors, title, journal, and PDF of
Manuscripts submitted but not yet accepted: (Provide names of all authors, title,
journal, and PDF of manuscript plus evidence of receipt of manuscript by journal.)
Invited or non-peer-reviewed articles or reviews: (Provide names of all authors,
year, title, journal, volume, and pages. If in press, provide documentation and PDF
or article/review if published within the last five years.)
226
title, edition, publisher, and pages. If in press, provide documentation and PDF or
book/book chapter if published the last five years.)
Nadeau, O.W. and Carlson, G.M. (2012) Methods for Detecting Structural Changes in
Large Protein Complexes.
Methods in Molecular Biology, Vol. 796 (Allostery: Methods and Protocols; A.W. Fenton,
Ed.), pp. 117-132, Humana Press, Totowa, NJ.
Published abstracts: (Provide names of all authors (applicant’s name bolded), year,
title, where published, volume, and pages.) If the same work is reflected in a published
abstract and a presentation or poster, the work must only be listed only once.
1. Nadeau, O.W. and Woodworth, R.C. (1990) A Bromopyruvate Affinity Labeled Peptide
From The N-Terminal
Half-Molecule of Ovotransferrin, Poster session and program abstracts, Fourth
Symposium of the Protein
Society.
2. Nadeau, O.W., Meyer, D.H. and Gump, D.W. (1990) Invasion of Epithelial Cells by
Enteroinvasive Escherichia
coli is Inhibited by Bismuth Subsalycilate, Poster session, American Society of
Microbiology, Abstracts of annual
meeting.
3. Gump, D.W., Nadeau, O.W. and Meyer, D.H. (1990) Invasion of Epithelial Cells by
Yersinia enterocolitica is
th
Inhibited by Bismuth Subsalycilate, Program abstract of the 30 Interscience Conference
Antimicrobial Agents &
Chemotherapy, Abstract 269.
4. Nadeau, O.W. and Carlson, G.M. (1993) Intramolecular Cross-linking of Phosphorylase
Kinase with
Transglutaminase, FASEB Journal, 7:A638.
5. Nadeau, O.W. , Sacks, D.B. and Carlson, G.M. (1995) Differential Affinity Cross-linking of
Phosphorylase Kinase
by Phenylenedimaleimide Isomers, Protein Science 4:147.
6. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1995) Exogenous Calmodulin Promotes
Changes in the
Quaternary Structure of Phosphorylase Kinase, FASEB Journal 9:A1304
2+
7. Nadeau, O.W. and Carlson, G.M. (1996) Ca
the Catalytic Subunit of
and Calmodulin Alter Interactions Between
Phosphorylase Kinase and the C-terminal Domain of its Regulatory α-subunit, FASEB
Journal 10:A1268.
227
8. Ayers, N.A., Nadeau, O.W., Read, M.W., Ray, P. and Carlson, G.M. (1997) Chemical
Cross-linking of
Phosphorylase-b Kinase by the Novel Cross-linker 4-phenyl-1,2,4-triazoline-3,5-dione,
FASEB Journal 11:A1180.
9. Nadeau, O.W., Traxler, K.W. and Carlson, G.M. (1997) The β Subunit of Phosphorylase
Kinase Interacts with the
N-terminal Domain of its Regulatory α Subunit, Protein Science 6:146.
10. Nadeau, O.W., Carlson, G.M. and Gogol, E.P. (2001) Structural Evidence for a Global
2+
Ca -dependent
Conformational Change in Phosphorylase Kinase, Protein Science 10:449.
11. Jeyasingham, M., Nadeau, O.W. and Carlson, G. M. (2002) Zero-length cross-linking of
the intrinsic catalytic
gamma subunit of phosphorylase kinase with either of its regulatory α or δ subunits is
2+
oppositely affected by Ca ,
Protein Science 11:268.
12. Nadeau, O.W., Gogol, E.P. and Carlson, G. M. (2003) Cryo-electron Microscopy Reveals
New Features in the
3D-Structure of Phosphorylase kinase, Protein Science 12:156.
13. Nadeau, O.W., Anderson, D. B., Yang, Q., Artigues, A. and Carlson, G.M. (2004) The Nterminus of the
regulatory β subunit of phosphorylase kinase mediates self-association of this subunit in
the activated
holoenzyme complex, Protein Science 13, Abs. # 83, 77.
14. Nadeau, O.W., Paschall, J.E., Wyckoff, G.J., Artigues, A. and Carlson, G.M. (2005) A
data-based approach for
detecting protein-protein interactions by chemical cross-linking and mass spectrometry,
Protein Science 14,
Abs. # 448, 215.
15. Nadeau, O.W., Paschall, J.E., Wyckoff, G.J., Artigues, A. and Carlson, G. (2006)
Mapping a Phosphorylationdependent Subunit Communication Network in the Hexadecameric Phosphorylase kinase
Complex Using a
Data-based Search engine to Detect Chemically Cross-linked Peptides by Mass
Spectrometry. Protein Science
15, Abs. # 457, 224.
16. Boulatnikov, I.G., Daniels, P.J., Nadeau, O.W., Kumar, P., Walsh, D.A., and Carlson,
G.M. (2006) Éxpressed αγδ
228
Subcomplex of the Phosphorylase b Kinase (PhK) Complex as a Model for
Characterizing the PhK (αβγδ) 4
Holoenzyme from Rabbit Skeletal Muscle. Protein Science 15, Abs. # 576, 268.
Gerald M. Carlson, (2007) Detection of Intrasubunit Interactions in the Regulatory β
Subunit in the
Hexadecameric Phosphorylase Kinase Complex Using a Data-based Search Engine to
Detect Chemically Crosslinked Peptides by Mass Spectrometry, Protein Sci. 16 (Suppl. 1), 249
Gerald M. Carlson, (2007) Detection of Intrasubunit Cross-linking Interactions in the
Regulatory Beta Subunit of
Phosphorylase Kinase Suggest a Possible Flip-flop Mechanism of Activation by
Phosphorylation, Eighth
International Symposium on Mass Spectrometry in the Life Sciences, C.16
19. Owen W. Nadeau, Laura Lane, Carol V. Robinson and Gerald M. Carlson, (2010) Mass
spectrometry reveals a
subunit interaction map of the phosphorylase kinase complex, Protein Sci. 19 (Suppl. 1),
250.
20. Owen W. Nadeau, Weiya Liu, Dong Xu, Todd Funke, Jessica Sage, Yang Zhang and
Gerald M. Carlson, (2011)
The glucoamylase activity of the protein kinase phosphorylase kinase, Protein Sci. 20
(Suppl. 1), 180.
21. Presentations and posters in chronological order (oldest first, most recent last)
Oral paper presentations: (Provide names of all authors (applicant’s name bolded), title,
sponsoring organization, extent of peer-review, and location and date of presentation.)
Poster presentations in chronological order (oldest first, most recent last): (Provide
names of all authors (applicant’s name bolded), title, sponsoring organization, extent of
peer-review and location and date of presentation.)
229
CURRICULUM VITAE
Kenneth Richard Peterson
PERSONAL DATA
Position: Professor
Birthday: 5/15/56
Citizenship: United States
Married, two children
ADDRESS
Department of Biochemistry and
Molecular Biology
MSN 3030
School of Medicine
Kansas City, Kansas 66160-7421
Tel.: (913) 588-6907 (Office)
588-5447 (Lab)
588-9896 (FAX)
E-mail: [email protected]
EDUCATION
Ph.D. University of Arizona, 1987
Dissertation advisor: Dr. David W. Mount
230
Dissertation topic:
Escherichia coli K-12.
Characterization of mutations in the lexA gene of
M.S. Idaho State University, 1981
Thesis advisor: Dr. Larry D. Farrell
Thesis topic: Attempted indirect induction of prophage lambda in E. coli
by nicked or gapped non-operator DNA.
B.S.
Northern Arizona University, 1979
Majors: Microbiology, Chemistry
PROFESSIONAL APPOINTMENTS
2010
20032003-
1998-2003
1996-1998
Director, Center for Epigenetics and Stem Cell Biology, Institute for
Reproductive Health and Regenerative Medicine, University of Kansas
Medical Center, Kansas City, Kansas
Vice-Chairman, Department of Biochemistry and Molecular Biology,
University of Kansas Medical Center, Kansas City, Kansas
Professor, Department of Biochemistry and Molecular Biology and
Department of Anatomy and Cell Biology, University of Kansas Medical
Center, Kansas City, Kansas
Associate Professor, Department of Biochemistry and Molecular Biology
and Department of Anatomy and Cell Biology, University of Kansas
Medical Center, Kansas City, Kansas
Research Associate Professor of Medicine, Division of Medical Genetics,
Department of Medicine, University of Washington, Seattle, Washington.
1996-1998 Adjunct Research Associate Professor of Genetics, Department of
Genetics, University of Washington, Seattle, Washington.
1996
Adjunct Research Assistant Professor of Genetics, Department of
1992-1996 Research Assistant Professor of Medicine, Division of Medical
Genetics, Department of Medicine, University of Washington,
Seattle, Washington.
1991-1997 Director, Phosphorimager Analysis Facility, Markey Molecular
Medicine Center, University of Washington, Seattle, Washington.
1990-1992 Senior Fellow, Dr. George Stamatoyannopoulos, Division of Medical
1987-1990 Postdoctoral Research Associate, Dr. Margaret G. Kidwell,
Department of Ecology and Evolutionary Biology, University of
Arizona, Tucson, Arizona.
1981-1986 Graduate Research Assistant, Dr. David W. Mount, Department of
Molecular and Cellular Biology, University of Arizona, Tucson,
Arizona.
231
1979-1981 Graduate Teaching Assistant, Department of Microbiology and
Biochemistry, Idaho State University, Pocatello, Idaho.
MEMBERSHIP IN PROFESSIONAL SOCIETIES
American Society of Hematology, 2001American Association for Advancement of Science, 1993American Society for Microbiology, 1980American Society of Nephrology, 2004-2007
Genetics Society of America, 1988International Society for Stem Cell Research, 2004Sigma Xi, 1981-
HONORS AND AWARDS
Self Faculty Scholar award for research and mentoring, University of Kansas,
2001.
Investigator Research Award, University of Kansas Medical Center, 2003.
Fulbright Scholar grantee to Brazil, 2009-2010.
SERVICE ACTIVITIES
Departmental
Member, Graduate Committee, Department of Biochemistry and Molecular Biology,
University of Kansas Medical Center, 1998-present.
Member, Search Committee for Fluorescence Biochemist, Department of Biochemistry
and Molecular Biology, University of Kansas Medical Center, 2003.
Member, Search Committee for Regulatory Biochemist , Department of Biochemistry and
Molecular Biology, University of Kansas Medical Center, 2003.
Chairman, Search Committees for Nucleic Acids Biochemist, Department of Biochemistry
and Molecular Biology, University of Kansas Medical Center, 2003-2006.
232
Chairman, Graduate Committee, Department of Biochemistry and Molecular Biology,
University of Kansas Medical Center, 2003-2004.
Member, Biochemistry and Molecular Biology Study Section, Department of Biochemistry
and Molecular Biology, University of Kansas Medical Center, 2005-present.
Chairman, Appointments, Promotions and Tenure Committee, Department of
Biochemistry and Molecular Biology, University of Kansas Medical Center, 2005-present.
Member, Search Committee for Senior Molecular Biologist, Department of Biochemistry
and Molecular Biology, University of Kansas Medical Center, 2006-2007.
Member, BMB KLSIC Space Advisory Committee, Department of Biochemistry and
Molecular Biology, University of Kansas Medical Center, 2007-present.
Participant, BMB Heartland Undergraduate Biochemistry forum, Department of
Biochemistry and Molecular Biology, University of Kansas Medical Center, 2011-2012.
Institutional
Member, Medical Thesis Committee, University of Washington, 1994-1998.
Faculty participant, Genetic Approaches to Aging Training Grant administered by Peter
Rabinovitch and George M. Martin, Department of Pathology, University of Washington,
1996-1998.
Member, Committees to develop Program in Molecular Genetics and Therapeutics and
Program in Developmental Biology, Task Force for Research, University of Kansas
Medical Center, 1998, 2000.
Member, Graduate Faculty, University of Kansas Medical Center, 1998-present.
Member, Smith Mental Retardation and Developmental Disabilities Research Center
(MRDDRC), University of Kansas Medical Center, 1998-2007.
Member, Advisory Board for the Transgenic and Genetic Technologies Support Facility,
University of Kansas Medical Center, 1998-2004, 2005-present.
Co-Chair, 2009.
Chair, 2009-present.
Participant, Annual IGPBS Recruitment Weekend, University of Kansas Medical Center,
1999-2006.
Member, Harry R. Wahl Academic Society, University of Kansas Medical Center, 19992005.
233
Judge, Student Research Forum, University of Kansas Medical Center, 1999, 2002-2004,
2006-2010.
Member, Subcommittee to examine microarray technology at KUMC, 2000.
Member, Graduate Travel Committee, University of Kansas Medical Center, 2000-2005.
Member, Laboratory Animal Resources Review Committee, University of Kansas Medical
Center, 2000.
Member, Laboratory Animal Resources Director Search Committee, University of Kansas
Medical Center, 2000-2001.
Elected member, School of Medicine Research Committee, University of Kansas Medical
Center, 2000-2003.
Vice-Chairman, 2001-2002.
Chairman, 2002-2003.
Member, Faculty Council, University of Kansas Medical Center, 2000-2003, 2004-2010.
Vice Chair-elect, 2008-2009.
Vice Chair, 2009-2010.
Member, Microarray Core Advisory Committee, University of Kansas Medical Center,
2001.
Member, Molecular Virologist Faculty Search Committee, Dept. Microbiology, Molec.
Genetics & Immunology, University of Kansas Medical Center, 2002.
Member, Internal Advisory/Planning Committee, KUMC Center for Molecular Informatics,
University of Kansas Medical Center, 2002.
Member, Medical Genetics Subcommittee of the Education Council, University of Kansas
Member, Faculty Assembly Research Committee, University of Kansas Medical Center,
2002-2003.
Member, Executive Committee of the Medical Faculty, University of Kansas Medical
Center, 2002-2003, 2004-2005, 2008-2010.
Member, Internal Advisory Committee, Institute of Maternal Fetal Biology, University of
Kansas Medical Center, 2002-2010.
Member, Self Graduate Fellowship Selection Committee, University of Kansas, 20022004.
234
Member, Research Advisory Group, University of Kansas Medical Center, 2003.
Member, Admissions/Prematriculation Working Group, Medical Education Review,
Chairman, W. S. Sutton and Reathea Mae Resco Scholarships Selection Committee,
Member, Interdepartmental Graduate Program in Biological Sciences Advisory Board,
Member, Medical Center Hearings Committee, University of Kansas Medical Center,
2004.
Member, Advisory Committee for the Molecular Resource Facility, University of Kansas
Medical Center, 2004.
Member, School of Medicine Space Committee, University of Kansas Medical Center,
2004.
Member, Viral Pathogenesis Faculty Search Committee, Dept. Microbiology, Molec.
Genetics & Immunology, University of Kansas Medical Center, 2005-2006.
Full member, Kansas Masonic Cancer Research Institute (KMCRI), Basic Science
Research Program/Risk Factors for Carcinogenesis, University of Kansas Medical
Center, 2005-2009.
Associate member, University of Kansas Cancer Center (KUCC), Risk Factors for
Carcinogenesis Research Program, University of Kansas Medical Center, 2009-present.
Member, Facility Director Search Committee, Transgenic and Gene Targeting Facility,
Member, Cancer/Developmental Biology Search Committee, Dept. Molecular &
Integrative Physiology, University of Kansas Medical Center, 2005-2006.
Member, Peter T. Bohan Lecture Selection Committee, University of Kansas Medical
Center, 2005.
Member, Cancer and Developmental Biology Seminar Series Committee, Institute of
Maternal-Fetal Biology, University of Kansas Medical Center, 2005.
Chair, Institutional Human Stem Cell Research Oversight Committee, University of
Kansas Medical Center, 2006-present.
Reviewer, Biomedical Research Training Program, University of Kansas Medical Center,
2007.
Primary Mentor, Department of Pediatrics Faculty Mentoring Program, University of
Kansas Medical Center, 2007.
235
Design Director, siRNA Library Core, University of Kansas Cancer Center (KUCC),
Member, Bioinformatics Search Committee, University of Kansas Medical Center, 2008.
Co-Director, IGPS Module 3: Molecular Biology course, University of Kansas Medical
Center, 2008-2009.
Member, KUMC Budget Advisory Committee, University of Kansas Medical Center, 2009.
Member, Furlough Plan Development Committee, University of Kansas Medical Center,
2009-2010.
Member, Executive Research Board, Institute for Reproductive Health and Regenerative
Medicine, University of Kansas Medical Center, 2010-present.
Member, High Throughput Genomics Facility Advisory Committee, University of Kansas
Medical Center, 2011-present.
Member, Kidney Institute Internal Executive Advisory Committee, University of Kansas
Member, LCME Educational Resources Self-Study Committee, University of Kansas
Professional
Extramural scientific reviews:
Grants:
Ad hoc reviewer, Wellcome Trust grants.
Ad hoc reviewer, National Science Foundation grants.
State of Louisiana Board of Regents Support Fund Research and Development Program,
2000.
External Advisory Committee, University of South Alabama Comprehensive Sickle Cell
Center, 2001.
Member, Hall Grant Annual Progress Report Review Committee, Kansas City
metropolitan area, 2002.
Ad hoc reviewer, NIH MBRS SCORE Program grant proposal, 2002-2003.
236
Ad hoc reviewer, Muscular Dystrophy Campaign grant proposal, 2003.
Ad hoc member, NIH Hematopoiesis (HP) Study Section, 2003.
Ad hoc member, NIH Erythrocyte and Leukocyte Biology (ELB) Study Section, 2004.
Ad hoc member, NIDDK Grant Review Board ZDK1 GRB-B (M1) and ZDK1 GRB-B (M2),
2004.
Reviewer, UK Biotechnology and Biological Sciences Research Council grant proposal,
2004.
Ad hoc reviewer, Special Emphasis Panel ZRG1 HEME-B 02 M, Globin Gene
Transcription, Hemostasis and Thrombosis (HT) Study Section, 2005.
Ad hoc reviewer, Special Emphasis Panel, ZRG1 HEME-B 02 M, Hemoglobin
Stabilizing Protein, Hemostasis and Thrombosis (HT) Study Section,
2005.
Reviewer, Special Emphasis Panel, Assay Development for High Throughput
Molecular Screening RFA, NINDS, 2006.
Ad hoc reviewer, Special Emphasis Panel, ZDK1 GRB-9 (J1), Hematopoietic
Stem Cells, NIDDK, 2006.
Ad hoc reviewer, Special Emphasis Panel, ZDK1 GRB-6 (M3) 1, A Search for
Genes that Regulate Stem Cells, NIDDK, 2008.
Reviewer, Netherlands Genomics Initiative Horizon Program grant proposal,
2008.
Ad hoc reviewer, PPG Special Emphasis Panel, HLBP 1 Workgroup 014,
Cryopreserved Erythroblast Products for Human Transfusion, NHLBI,
2009.
Ad hoc reviewer, Special Emphasis Panel, ZHL1 CSR-R (O5), Grand
Opportunity"(GO): Testing of Mechanistic Hypotheses Generated by
Findings from Genetic and Genomic Studies of Heart, Vascular, Lung, and
Blood Disorders, NHLBI, 2009.
Ad hoc reviewer, Special Emphasis Panel, ZHL1 CSR-R (O4), Grand
Opportunity"(GO): Next Steps in Gene Discovery: Building upon GWAS,
NHLBI, 2009.
Ad hoc reviewer, Special Emphasis Panel, ZHL CSR-R (F1), Patient Oriented
Research Career Enhancement Awards, NHLBI, 2009.
Ad hoc reviewer, Special Emphasis Panel, ZRG1 VH-F (03) M, Erythrocyte
Biology Teleconference, NIH CSR, 2010.
Ad hoc reviewer, Special Emphasis Panel, ZDK1 GRB-9 (M1), Hematology
Program Projects Teleconference, NIDDK, 2010.
237
Chair/Ad hoc reviewer, Special Emphasis Panel/Scientific Review Group 2011/01
ZRG1 CB-C (40) P, NIGMS P01 Internet Assisted Review, NIH CSR,
2010.
Ad hoc reviewer, Special Emphasis Panel/Scientific Review Group 2011/05
HLBP Workgroup 014, NHLBI P01 Review, NHLBI, 2011
Ad hoc reviewer, Special Emphasis Panel/Scientific Review Group 2011/05
ZDK1 GRB-6 (M3) 1, Hemoglobinopathies Program Projects
Teleconference, NIDDK, 2011.
Ad hoc member, NIH Molecular Genetics B (MGB) Study Section, 2012.
International Reviewer, Portuguese Foundation for Science and Technology,
2012.
Scientific Meetings:
Abstract reviewer, Thalassemia and Globin Gene Regulation Session, American Society
th
of Hematology 50 Anniversary Annual Meeting, 2008
Journals:
Guest Editor, Anemia, Sickle Cell Disease: Genetics, Cellular and Molecular
Mechanisms, and Therapies special issue, 2011-2012.
Ad hoc reviewer – American Journal of Hematology, Biochemical Genetics, Biochemical
Journal, BioTechniques, Biology of the Neonate, Blood, Blood Cells, Molecules and
Diseases, Cell, Cellular and Molecular Biology, Cellular and Molecular Biology Letters,
British Journal of Haematology, Cellular and Molecular Biology, Developmental
Dynamics, EMBO Journal, EMBO Reports, European Journal of Haematology,
Experimental Hematology, Genesis, Gene Therapy, Genetics, Genomics,
Haematologica, Human Molecular Genetics, Journal of Biological Chemistry, Journal of
Cellular Biochemistry, Journal of Molecular Medicine, Molecular and Cellular Biology,
Nature, Nature Protocols, Nucleic Acids Research, PLoS One, Proceedings of the
National Academy of Sciences USA, Transgenic Research.
Outside
Lectures on Hematology and Microbiology, Assumption-St. Bridget School, Seattle, WA,
1992-1998.
Co-founder and co-chairman, Assumption-St. Bridget School Science Parent
Club, 1997-1998. Organization received National Catholic Education
238
Association annual national award for Parents in Partnership with the
school, 1999-2000.
Scientist mentor, Science Education Partnership, grant-funded teacher-scientist program
of Fred Hutchinson Cancer Research Center, 1997, 1998.
Lectures on Molecular Biology and Genetics, Bentwood Elementary School, Overland
Park, KS., 1999, 2000, California Trail Jr. High, Overland Park, KS., 2000.
Judge, Kansas Junior Academy of Science District 3 and Shawnee Mission School
District Research and Development Forum, 1999, 2000, 2002-2004.
Judge, Shawnee Mission Research and Development Forum, 1999, 2000, 2002, 2003.
Judge, Science Pioneers Greater Kansas City Science & Engineering Fair, 2000-2004,
2006, 2009, 2012-2013.
Member, Proteomics Proposal Task Force, Kansas City Area Life Sciences Institute,
2001.
KUMC Representative, Kansas City Proteomics Consortium, Kansas City Area Life
Sciences Institute, 2001-2002.
Lecture, Science Pioneers’ Saturday Science Seminar program, 2003.
Co-Chairman, Stem Cell Symposium, University of Kansas Medical Center and Stowers
Institute for Medical Research, 2004.
Member, Program Committee, Stem Cells and Developmental Biology mini-symposium,
Life Sciences Research Day, Kansas City Area Life Sciences Institute, 2004-2005.
Teacher and Mentor, NHLBI Summer Institute Program to Increase Diversity (SIPID) –
Red Blood Cell Disorders, University of Texas at Dallas, 2007-2010.
Teacher and Mentor, PRIDE Summer Institute Programs to Increase Diversity among
Individuals Engaged in Health-Related Research, Georgia Health Sciences University,
2011-2012.
Lecture on Gene Regulation, Center for Advanced Professional Studies (CAPS), Blue
Valley School District, Overland Park, KS, 2012-2013.
RESEARCH SUPPORT
Extramural:
Current
239
PI:
1.
2.
1 R01 DK081290-A1, 09/01/08 – 08/31/12 (No cost extension to 08/31/13),
$212,500 (Annual direct costs), $850,000 (Total direct costs),
“Transactivation of Fetal Hemoglobin,” PI Kenneth Peterson.
1 X01 MH100830-01, 12/01/12-11/30/13, No annual direct costs, “HTS for
HbF Inducers in Human Beta-globin YAC Transgenic Mice Bone Marrow
Cells,” PI Kenneth Peterson
Co-PI, Co-I, Consultant, or Mentor:
1.
2 R01 HL069234-06A2, 09/01/08 – 05/31/12 (No cost extension to
05/31/13), $285,250 (Annual direct costs), $75,000 (Subcontract to Dr.
Peterson), “Gamma Globin Induction: Molecular and Cellular Based
Strategies,” Co-investigator with Betty S. Pace, University of Texas at
Dallas (5% effort).
2.
9 P20 GM104936-06, 09/01/12 – 06/30/17, $1,460,187 (Annual direct
costs), $7,263,467 (Total direct costs), “Molecular Regulation of Cell
Development and Differentiation,” Core C, Molecular Profiling Core Director
for Dale R. Abrahamson(10% effort).
3.
8 P20 GM103549-06, 07/01/11 – 06/30/16, $1,499,831 (Annual direct
costs), $7,499,155 (Total direct costs), “Nuclear Receptors in Liver Health
and Disease,” Consultant to Hartmut Jaeschke, mentor to Luciano
DiTacchio (4% effort).
Pending
PI:
1.
2.
1 R01 DK095448-01A1, 04/01/13 – 03/31/18, $250,000 (Annual direct
costs), $1,250,000 (Total direct costs), “Linkage Between Zinc Metabolism
and Fetal Hemoglobin Synthesis,” PI Kenneth Peterson.
1 R01 HL11126401-01A1, 07/01/13 – 06/30/18, $250,000 (Annual direct
costs), $1,250,000 (Total direct costs), “Mechanisms of HbF Activation by
Non-deletional HPFH,” PI Kenneth Peterson.
1.
1 R01 HL117993-01, 04/01/13 – 03/31/18, $250,000 (Annual direct costs),
$1,250,000 (Total direct costs), “Transcriptional Regulation of
Hematopoiesis by O-GlcNAcylation,” Multi-PI with Chad Slawson.
2.
AHA 13GRNT16910016, 07/01/13 – 06/30/15, $71,500 (Annual direct
costs), $143,000 (Total direct costs), “O-GlcNAcylation Regulates the
Mi2/NuRD Gene Repressor Complex,” Multi-PI with Chad Slawson.
3.
1 R01 DK100595-01, 09/01/13 – 08/31/16, $200,000 (Annual direct costs),
$600,000 (Total direct costs), “Regulation of Globin Gene Switching by OGlcNAc Post-Translational Modification,” Multi-PI with Chad Slawson.
Past
240
PI:
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
P01 HL053750, 09/30/94 – 09/29/99, $84,179 (Annual direct costs),
$405,532 (Total direct costs), “Gene Therapy for Sickle Cell Disease,”
Project 5, Production of a sickle cell mouse model, project PI Kenneth
Peterson.
R01 DK053510, 02/1/98 – 01/31/02 (No cost extension to 01/31/03),
$178,478 (Annual direct costs), $667,159 (Total direct costs), “Role of the
LCR in Human β-globin Gene Regulation,” PI Kenneth Peterson.
P50 DK057301, 09/30/99 – 08/31/01 (No cost extension to 08/31/02),
$50,000 (Annual direct costs), $100,000 (Total direct costs), “Kansas
Interdisciplinary Center for PKD Research,” Project 6, Test of the two-hit
hypothesis in a PKD mouse model, project PI Kenneth Peterson.
3 R01 HL067336-03S1 supplement, 09/22/03 – 05/31/05, $50,000 (Annual
direct costs), $100,000 (Total direct costs), “Regulation of Globin Gene
Switching in Human ES Cells,” PI Kenneth Peterson.
5 R01 HL067336, 06/15/01 – 05/31/05 (no-cost extension to 05/31/07),
$225,000 (Annual direct costs), $900,000 (Total direct costs), “Locus-linked
Regulatory Motifs of Globin Gene Switching,” PI Kenneth Peterson.
5 R01 DK061804, 09/30/01 – 05/31/05 (no-cost extension to 05/31/07),
$200,000 (Annual direct costs), $800,000 (Total direct costs), “Molecular
Control of Fetal γ-globin Gene Expression,” PI Kenneth Peterson.
Self Faculty Scholar Award, 07/1/01 – 06/30/04 (no cost extension to
06/30/07) $50,000 (Annual direct costs), $150,000 (Total direct costs),
"Studies of β-like Globin Gene Switching," PI Kenneth Peterson.
1 R56 DK081290, 09/24/07 – 08/31/08, $75,000 (Annual direct costs),
$75,000 (Total direct costs), “Transactivation of Fetal Hemoglobin,” PI
Kenneth Peterson.
AHA 0850193Z, 01/01/08 – 12/31/09, $71,500 (Annual direct costs),
$143,000 (Total direct costs), “TSPYL1: A Therapeutic Target for Treating
SCD-related Stroke,” PI Kenneth Peterson.
3 R01 DK081290-02S1, 01/18/10 – 12/31/10, $77,553 (Total direct costs),
“Transactivation of Fetal Hemoglobin, ARRA Administrative Supplement” PI
Kenneth Peterson.
2 R56 HL067336-05A2, 07/15/08-06/30/11, $250,000 (Annual direct costs),
$250,00 (Total direct costs), “Locus-linked Regulatory Motifs of Globin
Gene Switching,” PI Kenneth Peterson.
3 R01 DK081290-01A1S1, 07/01/09 – 10/31/11, $14,240 (Annual direct
costs), $24,480 (Total direct costs), “Transactivation of Fetal Hemoglobin,
ARRA Administrative Supplement for Students” PI Kenneth Peterson.
1.
R01 DK030852, 06/01/95 – 05/31/99, $328,700 (Annual direct costs),
$1,314,800 (Total direct costs), “Studies of Globin Gene Switching and
Erythroid Differentiation,” Co-investigator with Thalia Papayannopoulou.
2.
R01 HL046557, 12/01/95 – 11/30/99, $180,640 (Annual direct costs),
$722,560 (Total direct costs), “Biological Properties and Regulation of Stem
Cells,” Co-Investigator with Thalia Papayannopoulou.
241
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
R01 HL020899, 04/01/97 – 03/31/02, $281,508 (Annual direct costs),
$1,529,085 (Total direct costs), “Cellular Mechanisms of HbF Regulation,”
Co-Investigator with George Stamatoyannopoulos and Qiliang Li.
R01 DK045365, 08/01/97 – 07/31/02, $293,430 (Annual direct costs),
$1,589,309 (Total direct costs), “Molecular Control of Globin Gene
Switching,” Co-Investigator with George Stamatoyannopoulos and Qiliang
Li.
2 U54 HD033994, 04/01/01 – 03/31/06, $114,398 (Annual direct costs),
$607,355 (Total direct costs), "Center for Reproductive Sciences," Project
1, Regulation of SF1 in the Gonads, Co-investigator with Leslie L. Heckert
(5% effort).
3 P01 HD039878-03S1 supplement, 04/01/04 - 03/31/06, $75,000 (Annual
direct costs), $150,000 (Total direct costs), “Biology at the Maternal-Fetal
Interface,” Co-investigator with Michael J. Soares (5% effort).
FY2006-056 COBRE Center for Cancer Experimental Therapeutics Project
Award, 12/01/05 – 04/30/06, $52,000 (Annual direct costs), “Phenotypic
screens of the TGFβ tumor suppressor pathway in mouse ES cells,” Mentor
to Jay L. Vivian.
5 R01 AR047233, 08/01/00 – 07/31/05 (no-cost extension to 07/31/06),
$188,000 (Annual direct costs), $940,000 (Total direct costs), "Hoxc13 and
Hair Follicle Morphogenesis," Co-investigator with Alan R. Godwin (5%
effort).
P50 DK057301, 09/30/99 – 08/31/05 (no-cost extension to 08/31/06),
$144,567 (Annual direct costs), $675,356 (Total direct costs), “Kansas
Interdisciplinary Center for PKD Research,” Project 3, Polycystin G-protein
signal transduction, Co-investigator with James P, Calvet (5% effort).
5 R01 DK063975, 03/31/02 – 01/31/08, $189,636 (Annual direct costs),
$984,936 (Total direct costs), “A Mouse Model of Acrodermatitis
Enteropathica,” Co-investigator with Glen K. Andrews (10% effort).
5 P50 DK057301, 09/30/05 – 08/31/10, $137,500 (Annual direct costs),
$750,000 (Total direct costs), “Kansas Interdisciplinary Center for PKD
Research,” Project 2, Polycystin-1 mediated calcium and cAMP signaling,
Consultant to James P. Calvet (5% effort).
1 P20 RR021940, 06/01/06 – 04/30/11, $1,499,831 (Annual direct costs),
$7,499,155 (Total direct costs), “Nuclear Receptors in Liver Health and
Disease,” Consultant to Curtis D. Klaassen, mentor to Grace L. Guo and
Partha Krishnamurthy (8% effort).
1 P20 RR024214, 09/27/07 – 06/30/12, $1,500,000 (Annual direct costs),
$7,500,000 (Total direct costs), “Molecular Regulation of Cell Development
and Differentiation,” Core C, Molecular Biology Core Director for Dale R.
Abrahamson and mentor to Patrick E. Fields (20% effort).
Internal:
Current:
242
Past:
1.
Shared Use Biomedical Research Equipment, 11/22/99, $48,660,
“Purchase of a Qualitative and Quantitative PCR LightCycler System,”
University of Kansas Medical Center, Co-Applicant with Glen K. Andrews.
2.
Shared Use Biomedical Research Equipment, 11/20/03, $2,532, “Purchase
of Two Vertical Laminar Airflow Workstations in the Transgenic and Genetargeting Institutional Facility,” University of Kansas Medical Center, CoApplicant with Wenhao Xu and Alan R. Godwin.
3.
P20 Proposal Development, 08/05/04, $18,750, “Midwest Regional
Exploratory Center for Human Embryonic Stem Cell Research,” KUMC
Research Institute, University of Kansas Medical Center.
4.
Faculty Travel Award, 10/13/06, $779, 15th Conference on Hemoglobin
Switching, KUMC Research Institute, University of Kansas Medical Center.
5.
Bridging Grant Winter 2006 RFP, 04/01/06 – 03/31/07, $35,000, “Molecular
Control of Fetal γ-globin Gene Expression,” KUMC Research Institute,
University of Kansas Medical Center.
6.
Bridging Grant RFP, 04/18/06 – 04/30/07, $35,000, “Molecular Control of
Fetal γ-globin Gene Expression – Selection of Transactivators,” K-INBRE,
University of Kansas Medical Center.
7.
Faculty Travel Award, 09/24/07, $235, 49th American Society of
Hematology Annual Meeting, University of Kansas Medical Center.
8.
Faculty Travel Award, 10/10/08, $346, 50th American Society of
Hematology Annual Meeting, University of Kansas Medical Center.
9.
Bridging Grant Fall 2007 RFP, 01/01/08 – 12/31/08, $35,000,
“Transactivation of Fetal Hemoglobin,” KUMC Research Institute, University
of Kansas Medical Center.
10. Faculty Travel Award, 09/14/10,$700, 17th Hemoglobin Switching
Conference, KUMC Research Institute, University of Kansas Medical
Center.
11. Lied Endowed Basic Science Program, 02/01/10 – 01/31/11, $35,000,
“Transcriptional Regulation of the LAMA5 Gene,” University of Kansas
Medical Center, Co-investigator with Dale R. Abrahamson.
Pending:
PUBLICATIONS (88 total)
Papers in Peer Reviewed Journals
243
1.
Peterson, K. R., K. F. Wertman, D. W. Mount, and M. G. Marinus. 1985.
Viability of Escherichia coli DNA adenine methylase (dam) mutants requires
increased expression of specific genes in the SOS regulon. Mol. Gen.
Genet. 201:14-19.
2.
Peterson, K. R. and D. W. Mount. 1986. Differential repression of SOS
genes by unstable LexA41 (Tsl-1) protein causes a "split-phenotype" in
Escherichia coli K-12. J. Mol. Biol. 193:27-40.
3.
Peterson, K. R., N. Ossanna, and D. W. Mount. 1988. The Escherichia
coli K-12 lexA2 gene encodes a hypo-cleavable repressor. J. Bacteriol.
170:1975-1977.
4.
Ennis, D. G., K. R. Peterson, and D. W. Mount. 1988. Increased
expression of the Escherichia coli umuDC operon restores SOS
mutagenesis in lexA41 cells. Mol. Gen. Genet. 213:541-544.
5.
de Frutos, R., K. Kimura, and K. R. Peterson. 1989. In situ hybridization
of Drosophila polytene chromosomes with digoxigenin-dUTP labeled
probes. Trends in Genetics 5:336.
6.
Daniels, S. D., K. R. Peterson, L. D. Strausbaugh, M. G. Kidwell, and A.
Chovnick.
1990.
Evidence for horizontal transmission of the P
transposable element between Drosophila species. Genetics 124:339-355.
7.
de Frutos, R., K. Kimura, and K. R. Peterson. 1990. In situ hybridization
of Drosophila polytene chromosomes with digoxigenin-dUTP labeled
probes. Methods Mol. Cell. Biol. 2:32-36.
8.
Houck, M., J. Clark, K. R. Peterson, and M. G. Kidwell. 1991. Possible
horizontal transfer of Drosophila genes by the mite Proctolaelaps regalis.
Science 253:1125-1129.
9.
Wojciechowski, M. F., K. R. Peterson, and P. E. Love. 1991. Regulation
of the SOS response in Bacillus subtilis: Evidence for a LexA repressor
homolog. J. Bacteriol. 173:6489-6498.
10.
de Frutos, R., K. R. Peterson, and M. G. Kidwell. 1991. Distribution of
Drosophila melanogaster transposable element sequences in species of the
obscura group. Chromosoma 101:293-300.
244
11.
Gnirke, A., C. Huxley, K. Peterson, and M. V. Olson. 1993. Microinjection
of intact 200-500 kb fragments of YAC DNA into mammalian cells.
Genomics 15:659-667.
12.
Peterson, K. R., C. H. Clegg, C. Huxley, B. M. Josephson, H. S. Haugen,
T. Furukawa, and G. Stamatoyannopoulos. 1993. Transgenic mice
containing a 248 kb human β locus yeast artificial chromosome display
proper developmental control of human globin genes. Proc. Natl. Acad.
Sci. USA 90:7593-7597.
13.
Peterson, K. R. and G. Stamatoyannopoulos. 1993. Role of gene order in
the developmental control of human γ and β globin gene expression. Mol.
Cell. Biol. 13:4836-4843.
14.
Peterson, K. R. and D. W. Mount. 1993. Analysis of the genetic
requirements for viability of Escherichia coli K-12 DNA adenine methylase
(dam) mutants. J. Bacteriol. 175:7505-7508.
15.
Peterson, K. R., G. Zitnik, C. Huxley, C. H. Lowrey, A. Gnirke, K. A.
Leppig, T. Papayannopoulou, and G. Stamatoyannopoulos. 1993. Use of
YACs for studying control of gene expression: Correct regulation of the
genes of a human β-globin locus YAC following transfer to mouse
erythroleukemia cell lines. Proc. Natl. Acad. Sci. USA 90:11207-11211.
16.
Pace, B., Q. Li, K. Peterson, and G. Stamatoyannopoulos. 1994. α-amino
butyric acid fails to reactivate the totally silenced γ gene of the β locus YAC
transgenic mouse. Blood 84:4344-4353.
17.
Zitnik, G., K. R. Peterson, G. Stamatoyannopoulos, and T.
Papayannopoulou. 1995. Effects of butyrate and glucocorticoids on γ to β
globin gene switching in somatic cell hybrids. Mol. Cell. Biol. 15:790-795.
18.
Peterson, K. R., Q. Li, C. H. Clegg, T. Furukawa, P. A. Navas, E. J. Norton,
T. G. Kimbrough, and G. Stamatoyannopoulos. 1995. Use of yeast
artificial chromosomes (YACs) in studies of mammalian development:
Production of β-globin locus mice carrying human globin developmental
mutants. Proc. Natl. Acad. Sci. USA 92:5655-5659.
245
19.
Furukawa, T., P. A. Navas, B. M. Josephson, K. R. Peterson, T.
Papayannopoulou, and G. Stamatoyannopoulos. 1995. Coexpression of ε,
G
γ and Aγ globin mRNA in embryonic red blood cells from a single copy βYAC transgenic mouse. Blood Cells, Molecules, & Disease 21:168-178.
20.
Loring, J. F., C. Paszty, A. Rose, T. K. McIntosh, H. Murai, J. E. S. Pierce,
S. R. Schramm, E. M. Rubin, V. M.-Y. Lee, J. Q. Trojanowski, and K. R.
Peterson. 1996. Rational design of an animal model for Alzheimer’s
disease: Introduction of multiple human genomic transgenes to reproduce
AD pathology in a rodent. Neurobiol. Aging 17:173-182.
21.
Yang, Y., K. R. Peterson, T. Papayannopoulou, and G.
Stamatoyannopoulos. 1996. Human CD34+ cell EST database: Single
pass sequencing of 402 clones from a directional cDNA library. Exptl.
Hematology 24:605-612.
22.
Peterson, K. R., C. H. Clegg, P. A. Navas, E. J. Norton, T. G. Kimbrough,
and G. Stamatoyannopoulos. 1996. Effect of deletion of 5’HS3 or 5’HS2 of
the human β-globin locus control region on the developmental regulation of
globin gene expression in β-globin locus yeast artificial chromosome
transgenic mice. Proc. Natl. Acad. Sci. USA 93:6605-6609.
23.
Li, Q., C. Clegg, K. Peterson, N. Raich, and G. Stamatoyannopoulos.
1997. Regulation of the human β-globin locus by human GATA-1 as
measured in a binary transgenic mouse model. Proc. Natl. Acad. Sci. USA
94:2444-2448.
24.
Blau, C. A., K. R. Peterson, J. G. Drachman, and D. Spencer. 1997. A
proliferative switch for genetically modified cells. Proc. Natl. Acad. Sci.
USA 94:3076-3081.
25.
La Spada, A. R., K. R. Peterson, S. A. Meadows, M. E. McClain, G. Jeng,
R. S. Chmelar, H. A. Haugen, K. Chen, M. J. Singer, D. Moore, B. J. Trask,
K. H. Fischbeck, C. H. Clegg, and G. S. McKnight. 1998. Androgen
receptor YAC transgenic mice carrying CAG 45 alleles show trinucleotide
repeat instability. Human Mol. Genet. 7:959-967.
26.
Navas, P. A., K. R. Peterson, Q. Li, E. Skarpidi, A. Rohde, S. E. Shaw, C.
H. Clegg, H. Asano, and G. Stamatoyannopoulos. 1998. Developmental
specificity of the interaction between the locus control region and embryonic
or fetal globin genes in transgenic mice with an HS3 core deletion. Mol.
Cell. Biol. 18:4188-4196.
246
27.
Peterson, K. R. 1998. Rapid screening of recombinant DNA clones using
a single colony micro-lysate/restriction enzyme analysis protocol.
Biotechniques 25:26-28.
28.
Peterson, K. R., P. A. Navas, Q. Li, and G. Stamatoyannopoulos. 1998.
LCR-dependent gene expression in β-globin YAC transgenics: Detailed
structural studies validate functional analysis even in the presence of
fragmented YACs. Human Mol. Genet. 7:2079-2088.
29.
Vassilopoulos, G., P. A. Navas, E. Skarpidi, K. R. Peterson, C. H. Lowrey,
T. Papayannopoulou, and G. Stamatoyannopoulos. 1999. Correct function
of the locus control region may require passage through a nonerythroid
cellular environment. Blood 93:703-712.
30.
Papayannopoulou, T., G. V. Priestly, A. Rohde, K. R. Peterson, and B.
Nakamoto. 2000. Hemopoietic lineage commitment decisions: In vivo
evidence from a transgenic mouse model harboring µLCR-βpro-lacZ as a
transgene. Blood 95:1274-1282.
31.
Perkins, A. C., K. R. Peterson, G. Stamatoyannopoulos, H. E. Witkowska,
and S. H. Orkin. 2000. Fetal expression of a human Aγ globin transgene
rescues globin chain imbalance but not hemolysis in EKLF null mouse
embryos. Blood 95:1827-1833.
32.
Poorkaj, P., K. R. Peterson, and G. D. Schellenberg. 2000. Single-step
conversion of P1 and P1 artificial chromosome clones into yeast artificial
chromosomes. Genomics 68:106-110.
33.
Harju, S. J. and K. R. Peterson. 2001. Sensitive ribonuclease protection
assay
employing
glycogen
as
a
carrier
and
a
single
inactivation/precipitation step. BioTechniques 30:1198-1204.
34.
Navas, P. A., K. R. Peterson, Q. Li, M. McArthur, and G.
Stamatoyannopoulos. 2001. The 5'HS4 core element of the human βglobin locus control region is required for high level β-globin gene
expression in definitive erythroid cells. J. Mol. Biol. 312:17-26.
35.
Navas, P. A., Q. Li, K. R. Peterson, R. A. Swank, A. Rohde, J. Roy, and G.
Stamatoyannopoulos. 2002. Activation of the β-like globin genes is
dependent on the presence of the β-locus control region. Human Mol.
Genet. 11:893-903.
247
36.
Rodova, M., M. R. Islam, K. R. Peterson, and J. P. Calvet. 2003.
Remarkable sequence conservation of a small intron in the PKD1 gene.
Mol. Biol. Evol. 20:1669-1674.
37.
Navas, P. A., R. Swank, M. Yu, K. R. Peterson, and G.
Stamatoyannopoulos. 2003. Mutation of a transcriptional motif of a distant
regulatory element reduces the expression of embryonic and fetal globin
genes. Human Mol. Genet. 12:2941-2948.
38.
Peterson, K. R., H. Fedosyuk, L. Zelenchuk, B. Nakamoto, E. Yannaki, G.
Stamatoyannopoulos, S. Ciciotte, L. L. Peters, L. M. Scott, and T.
Papayannopoulou. 2004. Transgenic Cre expression mice for generation
of erythroid-specific gene alterations. Genesis 39:1-9.
39.
Harju, S. J., H. Fedosyuk, and K. R. Peterson. 2004. Rapid isolation of
yeast genomic DNA: Bust n’ Grab. MCB Biotechnol. 4:8-13.
40.
Fang, X., J. Sun, P. Xiang, M. Yu, P. A. Navas, K. R. Peterson, G.
Stamatoyannopoulos, and Q. Li. 2005. Synergistic and additive properties
of the β-globin LCR revealed by 5'HS3 deletion mutations: Implication on
LCR chromatin architecture. Mol. Cell. Biol. 25:7033-7041.
41.
Karpova, T., J. Presley, R. R. Manimaran, S. P. Scherrer, L. Tajeda, K. R.
Peterson, and L. L. Heckert. 2005. A Ftz-F1-containing yeast artificial
chromosome recapitulates expression of steroidogenic factor 1 in vivo.
Molec. Endocrinol. 19:2549-2563.
42.
Harju, S. J., P. A. Navas, G. Stamatoyannopoulos, and K. R. Peterson.
2005. Genome architecture of human β-globin locus affects developmental
regulation of gene expression. Mol. Cell. Biol. 25:8765-8778.
43.
Blau, C. A., C. F. Barbas, III, A. Bomhoff, R. Neades, J. Yan, P. A. Navas,
and K. R. Peterson. 2005. γ-globin gene expression in CID-dependent
multi-potential cells established from β-YAC transgenic mice. J. Biol.
Chem. 280:36642-36647.
44.
Navas, P. A., Q. Li, K. R. Peterson, and G. Stamatoyannopoulos. 2006.
Investigations of a human embryonic globin gene silencing element using
YAC transgenic mice. Exp. Biol. Med. 231:328-334.
45.
de Andrade, T. G., K. R. Peterson, A. F. Cunha, L. S. Moreira, A. Fattori,
S. T. O. Saad, and F. F. Costa. 2006. Identification of novel candidate
248
genes for globin regulation in erythroid cells containing large deletions of
the human β-globin gene cluster. Blood Cells, Mol. & Dis. 37:82-90.
46.
Fedosyuk, H. and K. R. Peterson. 2007. Deletion of the human β-globin
LCR 5’HS4 or 5’HS1 differentially affects β-like globin gene expression in βYAC transgenic mice. Blood Cells, Mol. Dis.39:44-55.
47.
Peterson, K. R. 2007. Preparation of YAC DNA for transgenesis of mice.
Nature Protocols 2:3009-3015.
48.
Harju-Baker, S.*, F. C. Costa*, H. Fedosyuk, R. Neades, and K. R.
Peterson. 2008. Silencing of Aγ-globin gene expression during adult
definitive erythropoiesis by GATA-1-FOG-1-Mi2 complex binding at the -566
GATA site. Mol. Cell. Biol. 28:3101-3113. PMCID: PMC2423176 *co-1st
authors
49.
Chin, J. Y., J. Y. Kuan, P. S. Lonkar, D. S. Krause, M. M. Seidman, K. R.
Peterson, P. E. Nielsen, R. Kole, and P. M. Glazer. 2008. Correction of a
splice-site mutation in the β-globin gene stimulated by triplex-forming
peptide nucleic acids. Proc. Natl. Acad. Sci. USA 105:13514-13519.
PMCID: PMC2533221.
50.
Desmarets, M., C. M. Cadwell, K. R. Peterson, R. Neades, and J. C.
Zimring.
2009.
Minor histocompatibility antigens on transfused
leukoreduced units of red blood cells induce bone marrow transplant
rejection. Blood 114:2315-2322. PMCID: PMC2745850
51.
Tschulena, U., K. R. Peterson, H. Fedosyuk, B. Gonzalez, and C. F.
Barbas III. 2009. Positive selection of DNA-protein interactions in
mammalian cells through phenotypic coupling with retrovirus production.
Nature Struct. Mol. Biol. 16:1195-1199. PMCID: PMC2880176
52.
Heruth, D. P., T. Hawkins, M. I. Gibson, D. P. Logsdon, I. V. Sokolovsky, N.
N. Nsumu, S. L. Major, B. Fegley, G. M. Woods, K. B. Lewing, K. A. Neville,
K. Cornetta, K. R. Peterson, and R. A. White. 2010. Mutation in erythroid
specific transcription factor KLF1 causes hereditary spherocytosis in the
Nan hemolytic anemia mouse model. Genomics 96:303-307.
53.
Chen, J., K. R. Peterson, C. Iancu-Rubin, and J. J. Bieker. 2010. Design
of embedded chimeric peptide nucleic acids that efficiently enter and
accurately target and reactivate gene expression in vivo. Proc. Natl. Acad.
Sci. USA 107:16846-16851. PMCID: PMC2947906
249
54.
Banzon, V., V. Ibanez, K. Vaitkus, M.-A. Ruiz, K. Peterson, J. DeSimone,
and D. Lavelle. 2011. siDNMT1 increases γ-globin expression in chemicalinducer-of-dimerization (CID)-dependent mouse β-YAC bone marrow cells
and in baboon erythroid progenitor cell cultures. Exptl. Hematol. 239:26-36.
55.
Giardine, B., J. Borg, D. R. Higgs, K. R. Peterson, S. Philipsen, D. Maglott,
B. K. Singleton, D. J. Anstee, A. N. Basak, B. Clark, F. C. Costa, P.
Faustino, H. Fedosyuk, A. E. Felice, A. Francina, M. V. E. Gallivan, M.
Georgitsi, R. J. Gibbons, P. C. Giordano, C. L. Harteveld, J. D. Hoyer, P.
Joly, E. Kanavakis, P. Kollia, S. Menzel, W. Miller, K. Moradkhani, J. Old, A.
Papachatzopoulou, M. N. Papadakis, P. Papadopoulos, S. Pavlovic, M.
Radmilovic, C. Riemer, I. Schrijver, M. Stojiljkovic, S. L. Thein, J. TraegerSynodinos, R. Tully, T. Wada, J. Waye, C. Wiemann, B. Zukic, D. H. K.
Chui, H. Wajcman, R. C. Hardison, and G. P. Patrinos. 2011. Systematic
documentation of human genetic variation using the microattribution
approach. Nature Genet. 43:295-301.
56.
Steenhard, B. M., A. Zelenchuk, L. Stroganova, K. Isom, P. L. St. John, G.
K. Andrews, K. R. Peterson, and D. R. Abrahamson. 2011. Transgenic
expression of human LAMA5 suppresses murine Lama5 mRNA and
Laminin α5 protein deposition.
PLoS ONE 6:e23926.
PMCID:
PMC3168496
57.
Costa, F. C., H. Fedosyuk, R. Neades, J. Bravo de los Rios, C. F. Barbas
III, and K. R. Peterson. 2012. Induction of fetal hemoglobin in vivo
mediated by a synthetic γ-globin zinc finger activator.
Anemia
2012:e507894. doi:10.1155/2012/507894. PMCID: PMC3384929
58.
Peterson, K. R., H. Fedosyuk, and S. Harju-Baker. 2012. LCR 5’
hypersensitive site specificity for globin gene activation within the active
chromatin
hub.
Nucleic
Acids
Res.
40:11256-11269.
doi:10.1093/nar/gks900. PMCID: PMC3526258
59.
Costa, F. C., H. Fedosyuk, A. M. Chazelle, R. Y. Neades, and K. R.
Peterson. 2012. Mi2β is required for γ-globin gene silencing: Temporal
assembly of a GATA-1-FOG-1-Mi2 repressor complex in β-YAC transgenic
mice.
PLoS Genet. 8:e1003155.
doi:10.1371/journal.pgen.1003155.
PMCID: PMC3527334
Reviews (Peer-reviewed)
1.
Ossanna, N., K. R. Peterson, and D. W. Mount. 1986. Genetics of DNA
repair in Bacteria. Trends in Genetics 2:55-58.
250
2.
Ossanna, N., K. R. Peterson, and D. W. Mount. 1987. UV-inducible SOS
response in Escherichia coli. Photochem. and Photobiol. 45:905-908.
3.
Peterson, K. R., N. Ossanna, A. T. Thliveris, D. G. Ennis, and D. W.
Mount. 1988. Derepression of specific SOS genes promotes DNA repair
and mutagenesis in Escherichia coli. J. Bacteriol. 170:1-4.
4.
McCormick, S. P. A., K. R. Peterson, R. E. Hammer, C. H. Clegg, and S.
G. Young. 1996. Generation of transgenic mice from yeast artificial
chromosome DNA modified by gene targeting. Trends Cardiovasc. Med.
6:16-24.
5.
Peterson, K. R., C. H. Clegg, Q. Li, and G. Stamatoyannopoulos. 1997.
Production of transgenic mice with yeast artificial chromosomes. Trends in
Genetics 13:61-66.
6.
Li, Q., S. Harju, and K. R. Peterson. 1999. Locus control regions: Coming
of age at a decade plus. Trends in Genetics 15:403-408.
7.
Harju, S. J., K. J. McQueen, and K. R. Peterson. 2002. Chromatin
structure and control of β-like globin gene switching. Exptl. Biol. Med.
227:683-700.
8.
Li, Q., K. R. Peterson, X. Fang, and G. Stamatoyannopoulos.
Locus control regions. Blood 100:3077-3086.
9.
Peterson, K. R.
2003.
Transgenic mice carrying yeast artificial
chromosomes. Expert Rev. Molec. Med. 5:1-25.
10.
Peterson, K. R.
2003.
Hemoglobin switching:
BloodMed.com December 24, 2003.
2002.
looping along.
Book Chapters and Invited Contributions
1.
Mount, D. W., K. F. Wertman, K. R. Peterson, J. W. Little, B. E. Markham,
and J. E. Harper. 1983. Regulation of the SOS response of Escherichia
coli by the lexA and recA genes. In: D. H. Hamer and M. J. Rosenberg
(ed.), Gene Expression, Alan R. Liss, Inc., New York, N.Y., pp. 135-143.
2.
Mount, D. W., K. F. Wertman, D. G. Ennis, K. R. Peterson, B. L. Fisher,
and G. Lyons. 1983. Genetic analysis of the SOS response of Escherichia
251
coli. In: E. Friedberg and B. A. Bridges (ed.), Cellular Responses to DNA
Damage, Alan R. Liss, Inc., New York, N.Y., pp. 343-352.
3.
Peterson, K. R., A. K. Ganesan, and D. W. Mount. 1986. Differential
expression of SOS genes in an E. coli mutant producing unstable LexA
protein enhances excision repair but inhibits mutagenesis.
In:
L.
Grossman and A. C. Upton (ed.), Mechanisms of DNA Damage and Repair,
Plenum Press, New York, N.Y., pp. 265-272.
4.
Kidwell, M. G., K. R. Peterson, and S. B. Daniels. 1990. Evolution of P
elements in the genus Drosophila. In: M. T. Clegg and S. J. O'Brian (ed.),
Molecular Evolution, Alan R. Liss, Inc., New York, N.Y., pp. 107-116.
5.
Kidwell, M. G. and K. R. Peterson. 1991. Evolution of transposable
elements in Drosophila. In: L. Warren and H. Koprowski (ed.), New
Perspectives on Evolution, Wiley-Liss, Inc., New York, N.Y., pp. 139-154.
252
6.
containing a 248-kilobase human β locus yeast artificial chromosome
display proper developmental control of human globin genes. Trans.
Assoc. Amer. Phys. 106:101-109.
7.
Castro, O. L., L. S. Chicoye, J. Greenberg, J. Haynes, Jr., and K. R.
Peterson. (S. Hart, ed.) 1994. Brighter horizons for sickle cell disease.
Patient Care 28:26-44.
8.
Stamatoyannopoulos, G., Q. Li., C. Clegg, and K. R. Peterson. 1995.
Molecular control of globin gene switching. In: Y. Beuzard, B. Lubin, and J.
Rosa (ed.), Sickle cell disease and thalassemias: New trends in therapy,
John Libbey Eurotext, Montrouge, France, pp. 3-8.
9.
Peterson, K. R., C. H. Clegg, Q. Li, P. A. Navas, E. J. Norton, K. A. Leppig,
and G. Stamatoyannopoulos. 1995. Analysis of hemoglobin switching in
transgenic mice using β-globin locus yeast artificial chromosomes. In: G.
Stamatoyannopoulos (ed.), Proceedings of the. Ninth Conference on
Hemoglobin Switching, Intercept Ltd., U.K., pp. 45-58.
10.
Papayannopoulou, Th., G. Zitnick, K. R. Peterson, and G.
Stamatoyannopoulos. 1995. Analysis of hemoglobin switching in somatic
cell hybrids. In: G. Stamatoyannopoulos (ed.), Proceedings of the. Ninth
Conference on Hemoglobin Switching, Intercept Ltd., U.K., pp. 291-298.
11.
Peterson, K. R. 1997. Production and analysis of transgenic mice containing
yeast artificial chromosomes. In: J. K. Setlow (ed.), Genetic Engineering,
Principles and Methods, Vol. 19, Plenum Press, N. Y., pp. 235-255.
12.
Li, Q., K. R. Peterson, and G. Stamatoyannopoulos. 1998. Developmental control
of ε- and γ-globin genes. Ann. N. Y. Sci. 850:10-17.
13.
Peterson, K. R., P. A. Navas, and G. Stamatoyannopoulos. 1998.
transgenic mice for studying LCR function. Ann. N. Y. Sci. 850:28-37.
14.
Peterson, K. R. 1999. Use of YACs to express genes in transgenic mice.
Methods Enzymol. 306:186-203.
15.
Jakobovits, A., B. T. Lamb, and K. R. Peterson. 1999. Production of transgenic
mice with yeast artificial chromosomes. In: R. S. Tuan and C. W. Lo (ed.),
Methods in Molecular Biology, Vol. 136: Developmental Biology Protocols, Vol.
II, Humana Press, Totowa, N. J., pp. 435-453.
253
β-YAC
16.
Peterson, K. R. 2003. Hemoglobin switching: New insights. Curr. Opin.
Hematol. 10:123-129.
17.
Blau, C. A. and K. R. Peterson. 2006. Establishment of cell lines that
exhibit correct ontogenic stage-specific gene expression profiles from
tissues of YAC transgenic mice using chemically induced growth signals.
In: A. MacKenzie (ed.), Methods in Molecular Biology, Vol. 349: YAC
Protocols, 2nd ed., Humana Press, Totowa, N.J., pp. 163-173.
18.
Paw, B. H., S.-K. Choe, F. C. Costa, S. V. Sundar, and K. R. Peterson.
2006. Vertebrate Models for Sickle Cell Disease Research. In: B. S. Pace
(ed.), Renaissance of Sickle Cell Disease Research in the Genomic Era,
World Scientific and Imperial College Press, London, U.K., pp. 237-257.
19.
Pace, B. S., S. F. Ofori-Acquah, and K. R. Peterson. 2012. Sickle cell
disease: Genetics, cellular and molecular mechanisms, and therapies.
Anemia 2012:e143594. doi:10.1155/2012/143594. PMCID: PMC3432324.
Manuscripts Submitted
1.
Debrand, E., L. Chakalova, J. Miles, Y. Dai, B. Goyenechea, S. Dye, C. S.
Osborne, A. Horton, S. Harju-Baker, K. R. Peterson, and P. Fraser. 2012.
An intergenic non-coding RNA promoter required for histone modifications
in the human beta-globin chromatin domain. PLoS One (Submitted).
Manuscripts in Preparation
1.
Peterson, K. R., F. C. Costa, H. Fedosyuk, R. Y. Neades, A. M. Chazelle,
L. Zelenchuk, A. H. Fonteles, P. Dalal, A. Roy, R. Chaguturu, B. Li, and B.
S. Pace. 2013. A cell-based high-throughput screen for novel inducers of
fetal hemoglobin for treatment of sickle cell disease, Cooley’s anemia and
β-thalassemias. Blood (In preparation).
Published Abstracts
1.
Mount, D. W., J. W. Little, K. F. Wertman, K. Peterson, G. Lyons, and H.
Ginsberg. 1983. Genetics of the SOS response of E. coli. J. Cell.
Biochem. supplement 7B, UCLA Symposia abstracts:163.
2.
Peterson, K. R. and D. W. Mount. 1987. Repair of DNA double-strand
breaks in Escherichia coli K-12 DNA adenine methylase (dam-) mutants
254
occurs via a recBC-dependent or a recN-dependent pathway. Genetics
116:s38. Annual Meeting of Genetics Society of America, San Francisco,
CA.
3.
Kidwell, M. G., K. R. Peterson, and S. B. Daniels. 1989. Evolution of P
elements in the genus Drosophila. J. Cell. Biochem., supplement 13C,
UCLA Symposia abstracts:88.
4.
Peterson, K. R. and G. Stamatoyannopoulos. 1992. The temporal order of
gene expression during development is not determined by the spatial order
of genes in a locus. Am. J. Hum. Genet. 51:A54. Presented at Annual
Meeting of the American Society of Human Genetics, San Francisco, CA.
5.
Peterson, K. R., B. M. Josephson, and G. Stamatoyannopoulos. 1992.
The temporal order of γ and β globin gene expression is independent of
their spatial order. Blood 80:243a. Presented at the Annual Meeting of the
American Society of Hematology, Anaheim, CA.
6.
Zitnik, G., K. Peterson, G. Stamatoyannopoulos, and Th.
Papayannapoulou. 1992. Dexamethasone accelerates switching in human
fetal erythroid x MEL hybrids. Blood 80:81a. Annual Meeting of the
American Society of Human Genetics, San Francisco, CA.
7.
containing a 248 kb human β locus YAC display proper developmental
control of human globin genes. Clin. Res. 41:258a. Presented at the
Plenary Session of the Annual Meeting of the Association of American
Physicians, Washington, D.C.
8.
Peterson, K. R., G. Zitnik, C. H. Clegg, A. Gnirke, C. Huxley, K. A. Leppig,
T. Papayannopoulou, and G. Stamatoyannopoulos. 1993. Use of YACs for
studies of development: Correct developmental regulation of a 248 kb β
globin locus YAC following transfer into mouse erythroleukemia (MEL) cells
and into transgenic mice. Am. J. Hum. Genet. 53:A5. Presented at the
Plenary Session of the Annual Meeting of the American Society of
Human Genetics, New Orleans, LA.
9.
Blau, C. A., K. R. Peterson, and G. Stamatoyannopoulos.
Functional redundancy of silencers in the ε globin promoter.
255
1993.
Blood
82:219a. Annual Meeting of the American Society of Hematology, St.
Louis, MO.
10.
Furukawa, T., G. Zitnik, K. Leppig, K. Peterson, B. Josephson, Th.
Papayannopoulou, and G. Stamatoyannopoulos. 1993. The LCR can
interact with two genes of a single β globin locus. Blood 82:435a. Annual
Meeting of the American Society of Hematology, St. Louis, MO.
11.
Pace, B., Q. Li, K. Peterson, and G. Stamatoyannopoulos. 1993. On the
mechanism of γ gene induction by butyrate: Role of the LCR and evidence
that butyrate cannot reactivate in vivo silenced γ genes. Blood 82:312a.
Annual Meeting of the American Society of Hematology, St. Louis, MO.
12.
Peterson, K. R., C. H. Clegg, P. A. Navas, T. Furukawa, and G.
Stamatoyannopoulos. 1994. Evidence that YACs can be used for
functional studies of regulatory elements of multigenic loci: Production of
YAC transgenic mice with -117 Aγ HPFH phenotype. Blood 84:218a.
Presented at the Annual Meeting of the American Society of Hematology,
Nashville, TN.
13.
Peterson, K. R., P. A. Navas, C. H. Clegg, and G. Stamatoyannopoulos.
1995. Use of β-YAC mice for studying LCR function: Effect of 5’ HS 2 or
HS 3 deletions on globin gene expression during development. Blood
86:586a. Presented at the Annual Meeting of the American Society of
Hematology, Seattle, WA.
14.
Zitnik, G., K. R. Peterson, G. Stamatoyannopoulos, and T.
Papayannopoulou. 1995. The silencing of γ-globin gene expression in a βglobin locus YAC can be arrested by α-aminobutyric acid. Blood 86:587a.
Annual Meeting of the American Society of Hematology, Seattle, WA.
15.
La Spada, A. R., K. Peterson, G. Jeng, K. Chen, K. Fischbeck, and G. S.
McKnight. 1996. Instability of CAG repeat expansions introduced into
yeast artificial chromosomes. Am. J. Hum. Genet. 59:A53. Annual Meeting
of the American Society of Human Genetics, San Francisco, CA.
16.
Blau, C. A., K. R. Peterson, J. G. Drachman, and D. Spencer. 1996. A
drug dependent proliferative switch for genetically modified cells. Blood
88:542a. Annual Meeting of the American Society of Hematology, Orlando,
FL.
256
17.
Navas, P. A., C. H. Clegg, E. Skarpidi, K. R. Peterson, and G.
Stamatoyannopoulos. 1996. β-YAC transgenic mice carrying a deletion of
234 bp core sequence of hypersensitive site 3: Relevance to function of the
LCR. Blood 88:462a. Annual Meeting of the American Society of
Hematology, Orlando, FL.
18.
La Spada, A. R., K. Peterson, M. E. McClain, G. Jeng, S. A. Meadows, K.
Chen, M. Singer, B. Trask, K. H. Fischbeck, and C. H. McKnight. 1997.
CAG repeat expansions in androgen receptor YAC transgenic mice are
moderately unstable. Am. J. Hum. Genet. 61:A312. Annual Meeting of the
American Society of Human Genetics, Baltimore, MD.
19.
Navas, P. A., K. R. Peterson, A. Rohde, H. Asano, C. Clegg, E. Skarpidi,
and G. Stamatoyannopoulos. 1997. Transgenic mice with HS3 core
deletion: Direct evidence that specific sequences of the LCR have specific
developmental roles. Blood 90:274a. Annual Meeting of the American
Society of Hematology, San Diego, CA.
20.
Papayannopoulou, Th., B. Nakamoto, K. Peterson, and G. Priestley. 1997.
Functional in vivo evidence that an erythroid specific enhancer can be
activated in all types of hemopoietic progenitors with extinction later in white
cells but consolidation of expression in erythroid and megakaryocytic cells.
Blood 90:428a. Annual Meeting of the American Society of Hematology,
San Diego, CA.
21.
Peterson, K. R., Y. Ji, C. Fernandez-Kodama, and T. Papayannopoulou. 1997.
Deletion of a loxP-flanked human βS gene in erythroid cells following erythroidspecific Cre recombinase expression in transgenic mice. Blood 90:132a. Annual
Meeting of the American Society of Hematology, San Diego, CA.
22.
Peterson, K. R., C. H. Clegg, P. A. Navas, T. G. Kimbrough, G. M. Alvino,
and G. Stamatoyannopoulos. 1997. Cis-control of globin gene switching
resides in gene-specific sequences, rather than gene order. Blood
90:275a. Annual Meeting of the American Society of Hematology, San
Diego, CA.
23.
Vassilopoulos, G., P. Navas, E. Skarpidi, K. Peterson, and G.
Stamatoyannopoulos. 1997. Normal function of the LCR may require
activation in a non erythroid cell environment. Blood 90:274a. Annual
Meeting of the American Society of Hematology, San Diego, CA.
24.
Jin, L., N. Siritanaratkul, D. W. Emery, R. E. Richard, G. V. Priestley, K. R.
Peterson, K. Kaushansky, T. Papayannopoulou, and C. A. Blau. 1998.
257
Reversible immortalization of multipotential, primary hemopoietic cells.
Blood Cells, Molecules, & Disease 24:465. Eleventh Conference on
Hemoglobin Switching, Orcas Island, WA.
25.
Navas, P. A., K. R. Peterson, Q. Li, E. Skarpidi, A. Rohde, S. E. Shaw, C.
H. Clegg, H. Asano, and G. Stamatoyannopoulos. 1998. Transgenic mice
with HS3 core deletion: Evidence for developmental specificity of the
interaction between the LCR and the embryonic or fetal globin genes.
Blood Cells, Molecules, & Disease 24:508. Eleventh Conference on
26.
Peterson, K. R., P. A. Navas, C. H. Clegg, T. G. Kimbrough, G. M. Alvino,
A. Rohde, and G. Stamatoyannopoulos. 1998. Cis-control of globin gene
switching resides in gene-specific sequences, as well as in gene order.
Blood Cells, Molecules, & Disease 24:480-481. Eleventh Conference on
27.
Peterson, K. R., P. A. Navas, Q. Li, and G. Stamatoyannopoulos. 1998.
LCR-dependent gene expression in 248 Kb and 155 Kb β-globin YAC
transgenics.
Blood Cells, Molecules, & Disease 24:511.
Eleventh
Conference on Hemoglobin Switching, Orcas Island, WA.
28.
Sabatino, D. E., K. Peterson, A. P. Cline, L. J. Garrett, P. G. Gallagher, B.
G. Forget, G. Stamatoyannopoulos, and D. M. Bodine. 1998. Substitution
of the β-spectrin promoter for the Aγ-globin promoter in transgenic mice in a
cosmid construct allows expression of human β-globin mRNA in transgenic
mouse yolk sac derived red cells. Blood Cells, Molecules, & Disease
24:513. Eleventh Conference on Hemoglobin Switching, Orcas Island, WA.
29.
Vassilopoulos, G., P. A. Navas, E. Skarpidi, K. R. Peterson, C. H. Lowrey,
T. Papayannopoulou, and G. Stamatoyannopoulos. 1998. Correct function
of the LCR may require passage through a non-erythroid cellular
environment. Blood Cells, Molecules, & Disease 24:519. Eleventh
Conference on Hemoglobin Switching, Orcas Island, WA.
30.
Sabatino, D. E., K. Peterson, A. P. Cline, L. J. Garrett, P. G. Gallagher,
and D. M. Bodine. 1998. Substitution of the β-spectrin promoter for the Aγglobin promoter in a YAC construct allows expression of human β-globin in
yolk sac derived red cells in transgenic mice. Blood. Annual Meeting of the
American Society of Hematology, Miami Beach, FL.
31.
Peterson, K. R., P. A. Navas, and G. Stamatoyannopoulos. 1999. Ciscontrol of globin gene switching resides in gene-specific sequences, as well
258
as in gene order. Blood 94:416a. Annual Meeting of the American Society
of Hematology, New Orleans, LA.
32.
Peterson, K.R. 2000. Role of gene-specific sequences and gene order in
cis-regulation of globin gene switching. Blood Cells, Molecules & Diseases
26:502. Twelfth Conference on Hemoglobin Switching, Orcas Island, WA.
33.
Harju, S., P. A. Navas, G. Stamatoyannopoulos, and K. R. Peterson.
2000. Cis-control of globin gene switching resides in gene-specific
sequences, as well as in gene order. Blood Cells, Molecules & Diseases
26:517. Twelfth Conference on Hemoglobin Switching, Orcas Island, WA.
34.
Peterson, K. R., S. Harju, P. A. Navas, and G. Stamatoyannopoulos.
2002. Role of γ-globin gene silencing and chromatin sub-domains in globin
gene switching. Blood 100:47a. Annual Meeting of the American Society
of Hematology, Philadelphia, PN.
35.
Peterson, K.R., S. Harju, P. A. Navas, and G. Stamatoyannopoulos. 2003.
Role of γ-globin gene silencing and chromatin sub-domains in globin gene
switching.
Blood Cells, Molecules & Diseases 31:161.
Thirteenth
Conference on Hemoglobin Switching, Oxford, UK.
36.
Harju, S., P. A. Navas, G. Stamtoyannopoulos, and K. R. Peterson. 2003.
Role of γ-globin gene silencing and chromatin sub-domains in globin gene
switching. Blood Cells, Molecules & Diseases 31:145-146. Thirteenth
37.
Harju, S., K. J. McQueen, H. Fedosyuk, and K. R. Peterson. 2003.
Deletion of LCR 5’HS4 does not markedly affect β-like globin gene
expression in β-YAC transgenic mice. Blood Cells, Molecules & Diseases
31:145. Thirteenth Conference on Hemoglobin Switching, Oxford, UK.
38.
Peterson, K. R., J. Yan, P. A. Navas, and C. A. Blau. 2003. Establishment
of CID-dependent progenitor cell lines from β-YAC transgenic mice. Blood
Cells, Molecules & Diseases 31:161.
Thirteenth Conference on
Hemoglobin Switching, Oxford, UK.
39.
Navas, P. A., R. Swank, K. R. Peterson, and G. Stamatoyannopoulos.
2003. Mutation of a GT motif in the HS3 core element in transgenic mice:
Evidence for stage-specific role of GT6 in ε- and γ-globin gene expression
during development.
Blood Cells, Molecules & Diseases 31:156.
Thirteenth Conference on Hemoglobin Switching, Oxford, UK.
259
40.
Peterson, K. R., J. Yan, P. A. Navas, and C. A. Blau. 2003. γ-globin gene
expression in CID-dependent multi-potential cell lines established from βYAC transgenic mice. Blood 102:266a. Annual Meeting of the American
Society of Hematology, San Diego, CA.
41.
Fang, X., J. Sun, G. Barkass, P. A. Navas, K. R. Peterson, G.
Stamatoyannopoulos, and Q. Li. 2003. The 5’HS3 core element of the βglobin LCR is required for formation of hyperacetylated histone domains in
the LCR and δ/β-globin gene region during adult erythropoiesis. Blood
Diego, CA.
42.
Harju, S., H. Fedosyuk, and K. R. Peterson. 2004. Effect of LCR 5’HS4
and 5’HS1 deletions on β-like globin gene expression in β-YAC transgenic
mice. Blood 104:342a. Annual Meeting of the American Society of
Hematology, San Diego, CA.
43.
Peterson, K. R., H. Fedosyuk, and S. Harju. 2004. LCR 5’HS3 displays
specificity for ε-globin gene activation during primitive erythropoiesis and γglobin gene activation during fetal definitive erythropoiesis.
Blood
Diego, CA.
44.
Harju, S. and K. R. Peterson. 2004. In vivo and in vitro analysis of an Aγglobin gene silencer in human β-YAC transgenic mice. Blood 104:345a.
Annual Meeting of the American Society of Hematology, San Diego, CA.
45.
Peterson, K. R., J. Yan, P. A. Navas, C. F. Barbas, III, and C. A. Blau.
2005. γ-globin gene expression in CID-dependent multipotential cells
established from β-YAC transgenic mice. Blood Cells, Molecules &
Diseases 34:116. Fourteenth Conference on Hemoglobin Switching, Orcas
Island, WA.
46.
Harju, S., H. Fedosyuk, and K. R. Peterson. 2005. Effect of LCR 5’HS4
and 5’HS1 deletions on β-like globin gene expression in β-YAC transgenic
mice. Blood Cells, Molecules & Diseases 34:92. Fourteenth Conference
on Hemoglobin Switching, Orcas Island, WA.
47.
Harju, S. and K. R. Peterson. 2005. In vivo and in vitro analysis of an Aγglobin gene silencer in human β-YAC transgenic mice. Blood Cells,
Molecules & Diseases 34:92-93. Fourteenth Conference on Hemoglobin
Switching, Orcas Island, WA.
260
48.
Peterson, K. R., H. Fedosyuk, and S. Harju. 2005. LCR 5’HS3 displays
specificity for ε-globin gene activation during primitive erythropoiesis and γglobin gene activation during fetal definitive erythropoiesis. Blood Cells,
Molecules & Diseases 34:115-116. Fourteenth Conference on Hemoglobin
Switching, Orcas Island, WA.
49.
Fraser, P., L. Chakalove, J. Miles, D. Carter, A. Horton, E. Debrand, B.
Goyenechea, J. A. Mitchell, C. Kattamis, S. Harju, K. Peterson, and C. S.
Osborne. 2005. β-globin locus transcription. Blood Cells, Molecules &
Diseases 34:87. Fourteenth Conference on Hemoglobin Switching, Orcas
Island, WA.
50.
Peterson, K. R., H. Fedosyuk, and S. Harju. 2005. Human β-globin locus
control region hypersensitive site specificity for globin gene activation
during erythropoiesis. Blood 106:1010a. Annual Meeting of the American
Society of Hematology, Atlanta, GA.
51.
Costa, F. C., S. Harju, H. Fedosyuk, L. Zelenchuk, R. Neades, and K. R.
Peterson. 2006. Silencing of Aγ-globin gene expression during adult
definitive erythropoiesis is mediated by GATA-1 binding. Blood Cells,
Molecules & Disease 38:170-171. Fifteenth Conference on Hemoglobin
Switching, Oxford, UK.
52.
Peterson, K. R., H. Fedosyuk, and S. Harju. 2006. Human β-globin locus
control region hypersensitive site specificity for globin gene activation
during erythropoiesis. Blood Cells, Molecules & Disease 38:170. Fifteenth
53.
Lavelle, D., V. Banzon, T. Kousnetzova, M. Hankewych, K. R. Peterson,
and J. DeSimone. 2007. RNAi targeting MBD2 increases γ-globin
expression in a CID-dependent human β-YAC murine bone marrow cell
line. Blood 110:525a. Annual Meeting of the American Society of
Hematology, Atlanta, GA.
54.
Peterson, K. R., F. C. Costa, and S. Harju-Baker. 2007. Silencing of γglobin gene expression during adult definitive erythropoiesis is mediated by
a GATA-1 repressor complex. Blood 110:87a. Annual Meeting of the
American Society of Hematology, Atlanta, GA.
55.
Costa, F. C., H. Fedosyuk, R. Neades, L. Zelenchuk, G. C. Maniscalco, and
K. R. Peterson. 2008. Transactivation of γ-globin gene expression by
novel transcription co-factors: TSPYL1, FGIF and MTF-1. Blood Cells,
Molecules & Disease, in press. Sixteenth Conference on Hemoglobin
Switching, Asilomar, CA.
261
56.
Costa, F. C., H. Fedosyuk, L. Zelenchuk, and K. R. Peterson. 2008.
Modulation of GATA-1 repressor activity at the Aγ-globin-566 GATA silencer
during fetal/adult erythropoiesis. Blood Cells, Molecules & Disease, in
press. Sixteenth Conference on Hemoglobin Switching, Asilomar, CA.
57.
Peterson, K. R., H. Fedosyuk, and F. C. Costa. 2008. Hereditary
persistence of fetal hemoglobin: old, new and future mutations in the Aγglobin gene-proximal region. Blood 112:492a. Annual Meeting of the
American Society of Hematology, San Francisco, CA.
58.
Ibanez, V., V. Banzon, T. Kousnetzova, K. Vaitkus, K. Peterson, J.
DeSimone, and D. Lavelle. 2008. The role of DNA damage/stress
response pathways in the mechanism of action of decitabine. Blood
Francisco, CA.
59.
Stavrou, E. F., E. Lagadinou, E. Papapetrou, N. Zoumbos, C. Barbas III, K.
Peterson, and A. Athanassiadou. 2008. S/MAR based episomal vector
mediates induction of gamma-globin expression by a specific zinc-finger
activator in K562 and murine progenitor cells. In Press. European
Hematology Association Congress, Copenhagen, Denmark.
60.
Stavrou, E. F., E. Lagadinou, E. Papapetrou, N. Zoumbos, C. Barbas III, K.
Peterson, and A. Athanassiadou. 2008. S/MAR based episomal vector
mediates induction of gamma-globin expression by a specific zinc-finger
activator in K562 and murine progenitor cells. Human Gene Therapy
19:1113. European Society of Gene and Cell Therapy Annual Congress,
Brugge, Belgium.
61.
Costa, F. C., H. Fedosyuk, and K. R. Peterson. 2009. The -566 Aγ-globin
HPFH mutation disrupts temporal repression of fetal hemoglobin synthesis
during fetal liver definitive erythropoiesis. Blood 114:797. Annual Meeting
of the American Society of Hematology, New Orleans, LA. Also published
on-line in F1000 Poster Bank (http://posters.f1000.com/).
62.
Banzon, B., V. Ibanez, K. Vaitkus, K. Peterson, J. DeSimone, and D.
Lavelle. 2009. siRNA targeting DNA Methyltransferase increases ε- and γglobin expression. Blood 114:402. Annual Meeting of the American
Society of Hematology, New Orleans, LA.
262
63.
Stavrou, E. F., E. Lagadinou, N. Zoumbos, A. Spyridonidis, C. Barbas III, K.
Peterson, and A. Athanassiadou. 2010. Development of an S/MAR based
episomal vector of a specific zinc-finger activator that mediates gammaglobin gene activation, for the gene therapy of hemoglobinopathies.
Molecular Therapy 18:S132. Annual Meeting of the American Society of
Gene and Cell Therapy, Washington, DC.
64.
White, R. A., D. P. Heruth, T. Hawkins, D. Logsdon, M. Gibson, I.
Sokolovsky, N. Nsumu, S. Major, B. Fegley, G. Woods, K. Lewing, K.
Neville, K. Cornetta, and K. Peterson. 2010. Mutation in erythroid specific
transcription factor KLF1 causes hereditary spherocytosis in the Nan
(neonatal anemia) hemolytic anemia mouse model. Blood 116:1320.
Annual Meeting of the American Society of Hematology, Orlando, FL.
65.
Peterson, K. R., F. C. Costa, H. Fedosyuk, R. Neades, J. Bravo de los
Rios, A. Fonteles, L. Zelenchuk, P. Dalal, and G. C. Maniscalco. 2011.
Induction of fetal hemoglobin by transcriptional co-activators MTF-1 and
TSPYL1. Blood (In press). Annual Meeting of the American Society of
Hematology, San Diego, CA.
Electronic submissions
1.
McQueen, K. J. and K. R. Peterson. 2001. Mus musculus polycystin 1
(Pkd1) gene, exons 39 through 46 and partial cds; and tuberous sclerosis 2
(Tcs2) gene, partial cds. GenBank AF333927.
INVITED TALKS
1992 DNX Symposium on Hemoglobin, Princeton, NJ.
hemoglobin switching in transgenic mice.
Regulation of
1992 Eighth Hemoglobin Switching Conference, Orcas Island, WA. Importance
of spatial order of γ and β globin genes in temporal order of expression.
1994 Gladstone Institute of Cardiovascular Disease, University of California San
Francisco, San Francisco, CA. Use of yeast artificial chromosomes
(YACs) in the production of transgenic mice.
1994 GenPharm International, Mountain View, CA. Generation of transgenic
mice using YACs.
1994 Ninth Conference on Hemoglobin Switching,
Transgenic Mice Using β Locus YACs.
263
Orcas
Island, WA.
1995 Gordon Conference on Red Cells, Plymouth, NH. Production of β-globin
locus YAC transgenic mice carrying human globin developmental mutants.
1996 Engineered Animal Models, Baltimore, MD. Production of β-globin locus
YAC transgenic mice carrying human globin developmental mutants.
1996 Tenth Conference on Hemoglobin Switching, Orcas Island, WA. Use of
YACs for studies of switching.
1997 Seventh Cooley’s Anemia Symposium, Cambridge, MA.
transgenic mice for studying LCR function.
β-YAC
1998 Keystone Symposium on Genomics and Drug Discovery, Santa Fe, NM.
Analysis of developmental regulation in YAC transgenic mice.
1998 Distinguished Scientist Seminar, University of South Alabama, Mobile, AL.
Analysis of human β-globin gene regulation in YAC transgenic mice.
1998 Eleventh Conference on Hemoglobin Switching, Orcas Island, WA. Ciscontrol of globin gene switching resides in gene-specific sequences, rather
than gene order.
1998 Department of Microbiology, Molecular Genetics and Immunology,
University of Kansas Medical Center, Kansas City, KS. Cis-control of
globin gene switching.
1999 Department of Molecular and Integrative Physiology, University of Kansas
Medical Center, Kansas City, KS. Role of cis-linked sequences and locus
organization in regulation of β-globin gene expression.
1999 Department of Veterans Affairs Medical Center, Kansas City, MO.
Developmental regulation of β-globin gene expression.
2000 School of Biological Sciences, University of Missouri-Kansas City, Kansas
City, MO. Developmental regulation of β-globin gene expression: Role of
cis-linked sequences and locus organization.
2000 Twelfth Conference on Hemoglobin Switching, Orcas Island, WA. Role of
gene-specific sequences and gene order in cis-regulation of globin gene
switching.
2001 Department of Pharmacology, Toxicology and Therapeutics, University of
Kansas Medical Center, Kansas City, KS. Locus-linked regulatory motifs
of human β-globin gene switching.
2002 Thirteenth Conference on Hemoglobin Switching, Oxford, UK. Role of γglobin gene silencing and chromatin sub-domains in globin gene
switching.
2002 Stowers Institute for Medical Research, Kansas City, MO.
control of β-globin gene switching.
Molecular
2003 Department of Anatomy and Cell Biology, University of Kansas Medical
Center, Kansas City, KS.
Long-range regulation of globin gene
expression by the locus control region.
264
2003 Division of Genetic Medicine, Vanderbilt University, Nashville, TN.
Molecular control of human β-globin gene switching.
2003 Department of Biology, University of Louisiana at Lafayette, Lafayette, LA.
Molecular control of human β-globin gene switching.
2003 Investigator Research Award, University of Kansas Medical Center,
Kansas City, KS. Long-range activation of β-globin gene expression by
the locus control region.
2004 Fourteenth Conference on Hemoglobin Switching, Orcas Island, WA.
LCR HS Specificity for globin gene activation.
2004 Institute of Biomedical Sciences and Technology, University of Texas at
Dallas, Richardson, TX.
Cis-acting determinants of γ-globin gene
activation.
2005 Greater Kansas City Association of Family & Consumer Sciences and
Business Professionals in Family & Consumer Sciences, Kansas City,
MO. Science and ethics: The intertwined debate on stem cells using
technology to enhance quality of life.
2005 Division of Genetic Medicine, Vanderbilt University, Nashville, TN.
Molecular control of fetal globin gene expression.
2005 Kansas City Metropolitan Bar Association Annual Bench-Bar and
Boardroom Conference, Lake of the Ozarks, MO. The embryonic stem
cell research debate – conflicts in law, science and ethics.
2005 Region Eight Conference of the American Medical Student Association,
Kansas City, MO. The embryonic stem cell research debate – conflicts in
science, medicine, ethics, law, and public policy.
2006 Fifteenth Conference on Hemoglobin Switching, Oxford, UK. Silencing of
A
γ-globin gene expression during adult definitive erythropoiesis is
mediated by GATA-1 binding.
2007 Transcription, Chromatin and Cancer, Stowers Institute for Medical
Research, Kansas City, MO.
GATA-1-mediated silencing of fetal
hemoglobin expression.
2008 Sixteenth Conference on Hemoglobin Switching, Asilomar, CA.
Transactivation of γ-globin gene expression by novel transcription cofactors: TSPYL1, FGIF AND MTF-1.
2008 Intercultural Center, Kansas City Kansas Community College, Kansas
City, KS. Human stem cells.
2009 Department of Molecular and Integrative Physiology, University of Kansas
Medical Center, Kansas City, KS. Transactivation and repression of
fetal hemoglobin expression: a bimodal approach to treat sickle cell
disease.
265
2009 Department of Molecular and Cell Biology, University of Texas at Dallas,
Richardson, TX. Transactivation and repression of fetal hemoglobin
expression: a bimodal approach to treat sickle cell disease.
2010 Department of Biological Sciences, Kansas City Kansas Community
College, Kansas City, KS. Human Stem Cell Research.
2010 Department of Biology, University of Louisiana at Lafayette, Lafayette, LA.
Activation of silent genes for the treatment of disease: A paradigm from
globin gene switching.
2010 Seventeenth Conference on Hemoglobin
Induction of fetal hemoglobin by MTF-1.
Switching,
Oxford,
UK.
2011 Department of Anatomy and Cell Biology, University of Kansas Medical
Center, Kansas City, KS. Transcriptional activation of γ-globin: The
therapeutic approach for treatment of sickle cell disease.
2012 Department of Biochemistry and Molecular Biology, University of Florida,
Gainesville, FL. Fetal hemoglobin: regulation, activation and treatment for
sickle cell disease.
TEACHING ACTIVITIES (49 contact hours total)
Current Graduate Student Courses
GSMC 850, Proteins and Metabolism (2009-present), 6 contact hours
IGPBS 898, Introduction to Faculty Research (1998-present), 1 contact hour
BCHM 922, Advanced Molecular Genetics (2000-present), Course Coordinator
(2012), 17 contact hours
PATH 803, Stem Cell Biology, (2005-present), 2 contact hours
PATH 805, Epigenetics (2009-present), 3 contact hours
Current Medical Student Courses
Module 1, Foundations of Medicine, Small Group Author (2006-present), 2
contact hours
Module 1, Foundations of Medicine, Small Group Discussion (2006-present), 8
contact hours
Module 2, Genetics and Neoplasia, Lecture (2006-present), 4 contact hours
Module 2, Genetics and Neoplasia, Small Group Discussion (2006-present), 4
contact hours
266
Module 2, Genetics and Neoplasia, Homework Discussion (2006-present), 1
contact hour
Module 2, Genetics and Neoplasia, Review session (2006-present), 1 contact
hour
Previous Graduate Student Courses
BCHM 862, Literature Seminar (1999-2012), Course Coordinator, 10 contact
hours
BCHM 893, Module 3 of the IGPBS: Molecular Biology (2000-2008), 13 contact
hours; Co-Director of the course (2008)
BCHM 894, Module 4 of the IGPBS: Cell and Developmental Biology (20002005), 4 contact hours
BCHM 894, Module 4 of the IGPBS: Cell and Developmental Biology (20072008), 2 contact hours
Previous Medical Student Courses
BIOC 801/802 Medical Biochemistry Small Group Discussion (1999-2006), 21
contact hours
Medical Biochemistry 801/Medical Physiology 801/Introduction to Clinical
Medicine 801 PBL (2005), 4 contact hours (2 listed in Small Group
Discussion)
BIOC 802 Medical Biochemistry (1999-2006), 3 contact hours
Module 2, Genetics and Neoplasia, Large Group Discussion (2006-2011), 1
contact hour
Genetics lecture, Surgical Residents (2008), 1 contact hour
FELLOWS & STUDENTS DIRECTED/MENTORING
Postdoctoral Trainees
Patrick A. Navas, Division of Medical Genetics, University of Washington, 1993-1998.
267
Yuanen Ji, Division of Medical Genetics, University of Washington, 1995-1997.
Susan L. Harlocker, Division of Medical Genetics, University of Washington, 1998.
Auste Geddes, Department of Biochemistry and Molecular Biology, University of Kansas
Medical Center, 2001-2003. Biomedical Research Training Program Postdoctoral
Scholar, 2003.
Rita Lee, Department of Biochemistry and Molecular Biology, University of Kansas
Susanna Harju, Department of Biochemistry and Molecular Biology, University of Kansas
Shirin V. Sundar, Department of Biochemistry and Molecular Biology, University of
Flávia C. Costa, Department of Biochemistry and Molecular Biology, University of Kansas
Medical Center, 2005-2012. Biomedical Research Training Program Postdoctoral
Scholar, 2006-2008.
Doctoral Graduate Students
Susanna Harju, Department of Biochemistry and Molecular Biology, University of Kansas
Medical Center, 1998-2003. Ph.D., 10/03/03. Biomedical Research Training Program
Predoctoral Scholar.
Kellie J. McQueen, Department of Biochemistry and Molecular Biology, University of
Kansas Medical Center, 1999-2001. Biomedical Research Training Program Predoctoral
Scholar.
Rachel L. Grau, Department of Biochemistry and Molecular Biology, University of Kansas
Allen Chazelle, Department of Biochemistry and Molecular Biology, University of Kansas
Masters Graduate Students
Anna Nunn, Department of Biochemistry and Molecular Biology, University of Kansas
Medical Center, 2002-2007. M.S., 04/23/07.
268
Rotation Students
Yi Yang, Department of Genetics, University of Washington, 1997.
Huimin Jiang, Interdisciplinary Graduate Program in Biomedical Sciences (IGPBS),
nd
University of Kansas Medical Center, 2 rotation, 1998-1999.
Kellie J. McQueen, IGPBS, University of Kansas Medical Center, 2
nd
rotation, 1998-1999.
rd
Greg J. Bartelma, IGPBS, University of Kansas Medical Center, 3 rotation, 1999-2000.
rd
S. Joshua Langmade, IGPBS, University of Kansas Medical Center, 3 rotation, 19992000.
st
Irma Gresshoff, IGPBS, University of Kansas Medical Center, 1 rotation, 2000-2001.
st
Christina Newport, IGPBS, University of Kansas Medical Center, 1 rotation, 2001-2002.
Anna Nunn, IGPBS, University of Kansas Medical Center, 2
nd
Kara Wagoner, IGPBS, University of Kansas Medical Center, 2
nd
nd
Raymond Camahort, IGPBS, University of Kansas Medical Center, 2
2004.
rotation, 2003-
st
Andrew Ralya, IGPBS, University of Kansas Medical Center, 1 rotation, 2004-2005.
th
Nathan Bushue, IGPBS, University of Kansas Medical Center, 4 rotation, Fall 2011; comentored with Dr. Chad Slawson.
st
Allen Chazelle, IGPBS, University of Kansas Medical Center, 1 rotation, Fall 2011.
rd
Nancy Stiles, University of Kansas Medical Center, 3 rotation, Spring 2012.
Medical Student Trainees
Joel D. Ackerman, Summer Research Traineeship for Medical Students, University of
269
James M. Howard, Summer Research Traineeship for Medical Students, University of
Kansas Medical Center, 2001; Winner, Santiago Grisolia Award for Excellence in
Biochemistry Research by a Medical Student, 2002.
Bennett Berning, Summer Research Traineeship for Medical Students, University of
High School Students
David Nicholl, Mentorship Academy Program, Blue Valley School District, Overland Park,
KS, 1999-2000.
Parmita Dalal, Science Fair Project, Shawnee Mission Northwest High School, Lenexa,
st
KS, 2000-2002; Winner, Grand Award (and five other awards), 51 Greater Kansas City
Science and Engineering Fair, 2002; Fourth place (of 65) in Biology Division of
International Science Fair, 2002.
Maria Martinez, Volunteer, 2003.
Taras Zelenchuk, Volunteer and Employee, Shawnee Mission East High School, Prairie
Village, KS, 2003-2004.
Patrick Sturdivant, Volunteer and Employee, Olathe East High School, Olathe, KS, 2004.
Ali Heitmann, Employee, Liberty High School, Liberty, MO, 2004-2005.
Gabriella Maniscalco, Volunteer Internship and Employee, Oak Park High School,
Kansas City, MO, 2006-2008; Awarded Gold Medallion Diploma and Academic Honors.
Prarthana Dalal, Science Fair Project, Shawnee Mission East High School, Prairie
Village, KS, 2008-2011; First Place, Kansas-Nebraska-Oklahoma Regional Junior
Science and Humanities Symposium, 2009, 2010, 2011; Winner, Grand Award (and two
th
other awards), 58 Greater Kansas City Science and Engineering Fair, 2009; Winner,
th
Grand Award (and six other awards), 59 Greater Kansas City Science and Engineering
Fair, 2010; Technology Showcase Award, Award of Distinction, Vic Regnier Award,
Research and Development Forum, 2010; Special Award – AVASC Foundation, Fourth
place – Medicine and Health Sciences Category, International Science and Engineering
Fair, 2010; Regional Finalist, Siemens Competition, 2010; Winner, Grand Award (and two
th
other awards), 60 Greater Kansas City Science and Engineering Fair, 2011; First Place,
Junior Science and Humanities Symposium, 2011; Vic Regnier Award, Shawnee Mission
School District Research and Development Forum, 2011; Third place Grand Award –
Medical Research Category, Intel International Science and Engineering Fair, 2011; First
place, International BioGENEius Challenge, 2011.
Yuliya Matskevych, Employee, Shawnee Mission Northwest High School, Shawnee
Mission, KS, 2010-12.
270
Emily Binshtok, Volunteer, Blue Valley West High School, Overland Park, KS, 2011.
Gaspar Maisonet, Volunteer, Olathe Northwest High School, Overland Park, KS, 2012.
University Undergraduate Students
Hanluen Kao, Employee, University of Kansas, Lawrence, KS, 2003.
Taras Zelenchuk, Employee, Shawnee Mission East High School, Prairie Village, KS and
University of Kansas, Lawrence, KS, 2005-2006.
Patrick Sturdivant, Employee, University of Kansas, Lawrence, KS, 2005-2006; Recipient
of University of Kansas Cancer Center Summer Student Research Training Program
award, 2007.
Kelsey Simpson, Employee, University of Kansas, Lawrence, KS, 2008.
Kayleigh Peterson, Employee, University of Kansas, Lawrence, KS, 2008-2010.
Gabriella Maniscalco, Employee, St. Louis University, St. Louis, MO, 2009.
Johana, Bravo de los Rios, Employee, University of Kansas, Lawrence, KS, 2010.
Other
Julia Draper, Volunteer, Graduate of Rhodes College, Memphis, TN, 2011-2012.
Tyler J. Stephenson, Kidney Institute-funded, Laboratory-based Summer Research
Position, Graduate of Baker University, Baldwin City, KS, 2012.
Matt Parker, Graduate of University of Kansas, Lawrence, KS, 2013.
Dean’s Scholars Faculty Mentor
Trinh Le (Amy) Doan, University of Kansas Honors Program, Lawrence, KS, 2004-2006.
271
Visiting Scholars
Tiago de Andrade, Graduate Student, Molecular Biology and Gene Therapy
Laboratory, HEMOCENTRO (UNICAMP), Cidade Universitária Zeferino
Vaz, Campinas, Brazil, 2004.
Luciana Sarmento Moreira, Graduate Student, Molecular Biology and Gene Therapy
Laboratory, HEMOCENTRO (UNICAMP), Cidade Universitária Zeferino Vaz, Campinas,
Brazil, 2004.
Mentored Junior Faculty
Grace L. Guo, Ph.D., Department of Pharmacology, Toxicology and Therapeutics,
Aron W. Fenton, Ph.D., Department of Biochemistry and Molecular Biology, University of
Liskin Swint-Kruse, Ph.D., Department of Biochemistry and Molecular Biology, University
of Kansas Medical Center, 2004-2009.
Jay L. Vivian, Ph.D., Department of Pathology and Laboratory Medicine, University of
Mukta Sharma, M.D., M.P.H., Department of Pediatrics, University of Kansas Medical
Center, 2006-2009; Mentor, NHLBI Summer Institute Program to Increase Diversity
(SIPID) – Red Blood Cell Disorders, University of Texas at Dallas, 2007-2009; Primary
Mentor, Department of Pediatrics Faculty Mentoring Program, University of Kansas
Patrick E. Fields, Ph.D., Department of Pathology and Laboratory Medicine, University of
Partha Krishnamurthy, Ph.D., Department of Pharmacology,
Therapeutics, University of Kansas Medical Center, 2007-2011.
Toxicology
and
Xiaobo Zhong, Ph.D., Department of Pharmacology, Toxicology and Therapeutics,
Valerie Schroeder, M.D., Department of Pediatric Cardiology, University of Kansas
272
Jyoti Panicker, M.D., Department of Pediatric Hematology/Oncology, University of
Stephen Parnell, Ph.D. Department of Biochemistry and Molecular Biology, University of
Levi H. C. Makala, D.V.M., M.B.A., Ph.D., Department of Pediatrics,
Hematology/Oncology Section, Georgia Health Sciences University; PRIDE Summer
Institute Programs to Increase Diversity among Individuals Engaged in Health-Related
Research – Functional and Applied Genomics of Blood Disorders, Georgia Health
Sciences University, 2011-present.
Omar Aljitawi, M.D., Division of Hematology/Oncology, Department of Internal Medicine,
University of Kansas Medical Center, 2012-present.
Comprehensive Exam Committees
Student
Department
Mentor
Date
Susanna Harju
06/01/99
Biochem./Mol. Biol.
Kenneth R. Peterson
Huimin Jiang
05/08/00
Biochem./Mol. Biol.
Glen K. Andrews
Kellie J. McQueen
07/19/00
Biochem./Mol. Biol.
Kenneth R. Peterson
Julie A. Carlsten
10/24/00
Anatomy/Cell Biol.
Douglas E. Wright
Ryan Thummel
Molec./Integr. Physiol. Alan R. Godwin
11/30/01
Huaijin Zhou
01/17/02
Micro./Mol. Genet./Immun.
Brian P. Hermann
02/27/02
Molec./Integr. Physiol. Leslie L. Heckert
Jing Liu
Stowers Institute/
Arcady Mushegian
05/16/03
273
Joe Lutkenhaus
Peizhen Song
Withdrew
Molec./Integr. Physiol. Alan R. Godwin
Benjamin Weaver
04/19/05
Biochem./Mol. Biol.
Rachel Buckley
04/21/05
Stowers Institute/
Glen K. Andrews
Robb Krumlauf
Anatomy/Cell Biol.
Matthew Goering
05/17/05
Stowers Institute/
Jennifer Gerton
Biochem./Mol. Biol.
Laura Galinañes-García Stowers Institute/
Withdrew
Robb Krumlauf
Anatomy/Cell Biol.
Raymond Camahort
02/27/06
Stowers Institute/
Jennifer Gerton
Biochem./Mol. Biol.
Aaron Gottschalk
05/26/06
Stowers Institute/
Joan Conaway
Biochem./Mol. Biol.
Bang Shen
11/07/07
Joe Lutkenhaus
Subhashchandra Naik
05/08/09
Biochem./Mol. Biol.
Mark T. Fisher
Lu Chen
08/20/09
Stowers Institute/
Joan Conaway
Biochem./Mol. Biol.
Shuai Lu
11/03/10
Stowers Institute/
Jennifer Gerton
Biochem./Mol. Biol.
Valentine Agbor
11/08/10
274
Yi Feng
Pathology/Lab. Med.
Patrick E. Fields
11/15/10
Elizabeth Dille
12/09/10
Mauricio Vargas Uribe Biochem./Mol. Biol.
12/06/11
Alexey S. Ladokhin
Julie Mitchell
03/19/12
Thomas Yankee
Rushi Trivedi
05/11/12
Stowers Institute/
Joan Conaway
Biochem./Mol. Biol.
Allen Chazelle
Biochem./Mol. Biol.
Kenneth R. Peterson
Zhen Zhang
Biochem./Mol. Biol.
Chad Slawson
Nairita Roy
Molec./Integr. Physiol. Russell Swerdlow
Nehemiah Alvarez
Pathology/Lab. Med.
Patrick E. Fields
Dissertation/Thesis Committees
Student
Department
Mentor
Dates
Susanna Harju
10/01/98-10/03/03
Biochem./Mol. Biol.
Kenneth
R.
Peterson
Kellie J. McQueen
01/15/99-06/30/01
Biochem./Mol. Biol.
Kenneth
R.
Peterson
Huimin Jiang
02/19/01-04/02/04
Biochem./Mol. Biol.
Glen
K.
Andrews
S. Joshua Langmade
03/07/01-11/01/01
Biochem./Mol. Biol. (M.S.)
Glen
K.
Andrews
275
Huaijin Zhou
04/22/02-12/08/04
Joe
Anna Nunn
06/01/02-04/23/07
Biochem./Mol. Biol. (M.S.)
Kenneth
Ryan Thummel
Molec./Integr. Physiol.
06/24/02-06/25/04
Xinghao Wang
08/01/03
Stowers Institute/
Alan
Lutkenhaus
R.
R.
Peterson
Godwin
Robb Krumlauf 08/12/02-
Anatomy/Cell Biol. (M.S.)
Brian P. Hermann
02/24/03-10/04/05
Jing Liu
04/18/05
Molec./Integr. Physiol. Leslie
Stowers Institute/
L.
Arcady Mushegian
Heckert
05/16/03-
Dahzi Liu
05/24/04-12/05/05
Stowers Institute/
Ting Xie
Anatomy/Cell Biol. (M.S.)
Benjamin Weaver
04/19/05-04/03/09
Rachel Buckley
05/23/08
Biochem./Mol. Biol.
Stowers Institute/
Glen
K.
Andrews
Robb Krumlauf 04/21/05-
Anatomy/Cell Biol.
Matthew Goering
05/17/05-12/03/10
Stowers Institute/
Jennifer
Gerton
Biochem./Mol. Biol.
Daniel Kirilly
11/22/05-07/19/06
Stowers Institute/
Ting Xie
Anatomy/Cell Biol.
Raymond Camahort
02/27/06-12/14/08
Stowers Institute/
Biochem./Mol. Biol.
276
Jennifer
Gerton
Aaron Gottschalk
05/26/06-05/27/10
Stowers Institute/
Joan Conaway
Biochem./Mol. Biol.
Elizabeth Dille
present
Bang Shen
09/06/07-04/01/10
Joe
Subhashchandra Naik
03/31/08-11/29/12
Biochem./Mol. Biol.
Mark
Yi Feng
Pathology/Lab. Med.
11/12/08-09/18/12
Lu Chen
04/15/09-present
Patrick
Stowers Institute/
08/07/07-
Lutkenhaus
T.
Fisher
E.
Fields
Joan Conaway
Biochem./Mol. Biol.
Valentine Agbor
08/31/12
Shuai Lu
10/05/09-present
Stowers Institute/
04/17/09-
Jennifer
Gerton
S.
Ladokhin
Biochem./Mol. Biol.
Mauricio Vargas Uribe Biochem./Mol. Biol.
01/05/11-present
Rushi Trivedi
08/30/11-present
Alexey
Stowers Institute/
Joan Conaway
Biochem./Mol. Biol.
Julie Mitchell
10/03/11-present
Thomas
Yankee
Nairita Roy
10/19/11-04/02/12
Pathology/Lab. Med.
Soumen Paul
Molec./Integr. Physiol. Russell
Swerdlow
08/28/12-present
Allen Chazelle
05/11/12-present
Biochem./Mol. Biol.
277
Kenneth
R.
Peterson
Zhen Zhang
08/09/12-present
Biochem./Mol. Biol.
Chad Slawson
Nehemiah Alvarez
02/11/13-present
Pathology/Lab. Med.
Patrick
E.
Fields
International Dissertation/Thesis Examination Committees
2011 Yuen Tien Tan, PhD. dissertation, Mentor: Professor S. M. Jane, Department of
Medicine (RMH), University of Melbourne, Victoria, Australia
2013 Fiona Claire, Ph.D. dissertation, Co-mentors: Professor S. M. Jane and
Associate Professor D. J. Curtis, Department of Medicine (RMH), University of
Melbourne, Victoria, Australia
Educational Materials Development
Web materials for BIOC 802 lectures on developmental regulation, animal
models, gene therapy, and human stem cells, 2000-2006.
Syllabus for BCHM 893, IGPBS Module 3 lectures on RNA splicing, 2000-2008.
Syllabus for BCHM 894, IGPBS Module 4 lectures on gene regulation, 20012005.
Development and implementation of new advanced graduate curriculum,
Department of Biochemistry and Molecular Biology, 2003-2004.
Development and implementation of new BCHM 893, IGPBS Module 3
curriculum, 2004.
Syllabus for BCHM 893, IGPBS Module 3 lectures on DNA replication and repair,
2004-2008.
Sickle Cell Disease Small Group Discussion unit, Module 1, Foundations of
Medicine, 2006-2012.
Web materials for Module 2, Genetics and Neoplasia lectures and large group
discussions, 2006-2012.
Lectures and Laboratory Methods, NHLBI Summer Institute Program to Increase
Diversity (SIPID) – Red Blood Cell Disorders, University of Texas at
Dallas, 2007-2010.
Course schedule and paper discussion guidelines, BCHM 893, IGPBS Module 3,
2008.
278
Syllabus for GSMC 850, Proteins and Metabolism lectures on regulation of
chromatin structure, structure of chromosomes, genome evolution and
student literature discussion, 2009-2012.
Lectures and Laboratory Methods, PRIDE Summer Institute Programs to
Increase Diversity among Individuals Engaged in Health-Related
Research, Georgia Health Sciences University, 2011-2012.
Lecture, Center for Advanced Professional Studies (CAPS), Blue Valley School District,
Overland Park, KS, 2012-2013.
279
u
Curriculum Vitae
Chad Slawson
Office: (913) 945-6468
University of Kansas School of Medicine
Fax:
(913) 588-9896
3901 Rainbow Blvd. MS3030
Kansas City, KS 66160
Email:
[email protected]
Education
2007
Postdoctoral fellow in the laboratory of Dr. Gerald W. Hart, Department of Biological
Chemistry, The Johns Hopkins School of Medicine
2002
Ph.D. University of South Florida, Department of Chemistry
Chemistry
•
1994
Mentor: Robert Potter
Dissertation Title: Characterization of Novel Single Sugar Protein
Modifications in Proliferative Systems
B.S. Indiana University
Biochemistry
Professional Experience
•
November 2012 to present: Associate Member, Cancer Biology, The University of
Kansas Cancer Center
•
December 2011 to present: Member Institute for Reproductive Health and Regenerative
Medicine, The University of Kansas Medical Center
•
January 2011 to present: Assistant Professor in the Department of Biochemistry and
Molecular Biology, The University of Kansas Medical Center
•
August 2011 to June 2012: Consultant - Mediomics, LLC. St. Louis, MO
•
September 2007 to 2010:
Research Associate in the laboratory of Gerald W. Hart,
Department of Biological Chemistry, The Johns Hopkins School of Medicine
280
Awards and Honors
•
•
•
•
•
•
•
•
•
•
Best Thematic Poster: DNA Replication, Recombination, Repair presented by the JBC at
the 2012 Experimental Biology Meeting
Department of Biological Chemistry representative to Post-Doctorial Council, The Johns
Hopkins School of Medicine, Fall 2005
American Society for Biochemistry and Molecular Biology Travel Award, June 2004
st
Conference Chair, 1 Annual Raymond N. Castle Student Research Conference,
Department of Chemistry, University of South Florida, April 28, 2001
Graduate Representative to the Department of Chemistry, University of South Florida,
August 2000 – 2001
Tharpe Travel Award: Department of Chemistry, University of South Florida, November
2000
Tharpe Summer Fellowship: Department of Chemistry, University of South Florida, May
2000
Provost Teaching Award Honorable Mention: University of South Florida, April 2000
Tharpe Teaching Award: Department of Chemistry, University of South Florida, May 1998
Provost Teaching Award Nominee: University of South Florida, April 1998 and 1999
Professional Affiliation
•
•
•
•
•
American Heart Association – Member, 2013
American Society for the Advancement of Science – Member, 2011
Society for Glycobiology – Member, 2008
American Chemical Society – Member, 2007
American Society for Biochemistry and Molecular Biology – Member, 2006
Publications
1. Tan, E., Caro, S., Potnis, A., Lanza, C., and Slawson, C. (2013) O-GlcNAc
cycling regulates mitotic spindle organization. Sci. Signal (submitted).
2. McGreal, S., Bhushan, B., Walesky, C., Cleveland, J., Gallagher, T., Zhang, Z.,
Slawson, C., and Apte, A. (2013) Increased Hepatic O-GlcNAcylation Aggravates
Acetaminophen-induced Liver injury. Toxicological Sciences (In Review).
3. Gao, X., Wang, X., Pham, T.H., Feuerbacher, L.A., Lubos, M.L., Huang, M.,
Olsen, R., Mushegian, A., Slawson, C., and Hardwidge, P.R. (2013) NleB, a
bacterial effector with glycosyltransferase activity, targets GAPDH function to
inhibit NF-κB activation. Cell Host & Microbe 13:1-13. PMC3553500
281
4. Slawson, C., and Hart, GW. (2011) O-GlcNAc signaling: implications for
cancer cell biology. Nat. Rev. Cancer, 11: 678-684.
5. Hart, G.W., Slawson, C., Ramirez-Correa, G., and Lagerlof, O. (2011)
Crosstalk Between O-GlcNAcylation and Phosphorylation: Roles in
Signaling, Transcription, and Chronic Disease. Annual Reviews in
Biochemistry, 80: 825-858.
6. Wells, L.*, Slawson, C.*, and Hart, G.W. (2011) The E2F-1 Associated
Retinoblastoma-Susceptibility Gene Product is Modified by O-GlcNAc.
Amino Acids 40:877-883. * Each author contributed equally to the
manuscript.
7. Slawson, C., Copeland, R.J., and Hart, G.W. (2010) O-GlcNAc Siganling:
A Metabolic Link Between Diabetes and Cancer? Trends in Biochem.
Sci. 35:547-555.
8. Wang, Z.*, Udeshi, N.D.*, Slawson, C.*, Compton, P.D., Sakabe, K.,
Cheung, W.D., Shabanowitz, J., Hunt, D.F., and Hart G.W. (2010) OGlcNAcylation Regulates Mitotic Spindle/Midbody Phosphorylation. Sci
Signal 3, ra2. * Each author contributed equally to the manuscript.
Featured in a Science Signaling perspective, M. C. Hall, Proteomics
Modifies Our Understanding of Cell Cycle Complexity. Sci. Signal. 3, pe4
(2010).
9. Li, X., Molina, H., Huang, H., Zhang, Y-Y., Liu, M., Qian, S-W., Slawson,
C., Dias, W.B., Pandey, A., Hart, G.W., Lane, M.D., and Tang, Q-Q.
(2009) O-Linked N-acetylglucosamine modification on C/EBPβ: role during
adipocyte differentiation. J. Biol. Chem. 284:19248-19254.
10. Slawson, C. Lakshmanan, T., Knapp, S., and Hart, G.W. (2008) A mitotic
GlcNAcylation/Phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin. Mol. Biol. Cell
19:4130-4140. Selected for cover and previewed in ASCB InCyctes.
11. Hart, G.W., Housley, M.P., and Slawson, C. (2007) Cycling of O-Linked βN-acetylglucosamine on nucleocytoplasmic proteins. Nature 446:10171022.
12. Slawson, C., Housley, M.P., and Hart, G.W. (2006) O-GlcNAc cycling:
how a single sugar post-translational modification is changing how we
think about signaling networks. J. Cell. Biochem. 97:71-83. Selected for
cover.
13. Slawson, C., Zachara, N.E., Vosseller, K., Cheung, W.D., Lane, M.D., and
Hart, G.W. (2005) Perturbations in O-linked β-N-acetylglucosamine protein
282
modification cause severe defects in mitotic progression and cytokinesis.
J. Biol. Chem. 280:32944-32956.
14. Slawson, C., and Hart, G.W. (2003) Dynamic interplay between O-GlcNAc
and O-Phosphate: the sweet side of protein regulation. Curr. Opin.
Struct. Biol. 12:631-636.
15. Slawson, C., Shafii, S., Amburgey, J., and Potter, R.L.
(2002)
Characterization of the O-GlcNAc protein modification in Xenopus laevis
oocytes during oogenesis and progesterone stimulated maturation.
Biochim. Biophys. Acta 1573:121-129.
16. Slawson, C., Pidala, J., and Potter, R.L. (2001) Increased N-Acetyl-βglucosaminidase activity in primary breast carcinomas corresponds to a
decrease in N-acetylglucosamine containing proteins. Biochim Biophys
Acta 1537:147-57.
17. Slawson, C., Stewart, J., and Potter, R.L. (2001) Biocatalytic lactone
generation in genetically engineered E. coli and identification of products
by gas chromatography – mass spectroscopy. J. Chem. Edu. 78:15331544.
Research Funding
Ongoing Research Support:
1. P20 RR024214-04 (D. Abrahamson) Molecular Regulation of Cell Development and
Differentiation
This application establishes a multidisciplinary Center for Biomedical Research Excellence
(COBRE) with a developmental biology emphasis. Funds are requested to support 3 scientific
core facilities and an Administrative Core, 5 research projects, and provide for a strong junior
faculty recruiting and mentoring program. There are no funds in this grant that provide research
support for the Abrahamson laboratory.
Chad Slawson receives $147,000 (for up to 3 years) with 50% effort on the project “Targeting and
Regulation of O-GlcNAc Transferase at M Phase”.
2. P30AG035982 (R. Swedlow)
NIH/NIA
This application established the University of Kansas School of Medicine Alzheimer’s Center.
The goal of the KUADC is to support Alzheimer’s and brain aging research. Chad Slawson has
received a $30,000 pilot grant for initial research into how mitochondria disrupt O-GlcNAc
283
signaling in Alzheimer’s diseases. The title is the following: Alzheimer’s Mitochondria Defects
Alter O-GlcNAc Signaling
Invited Seminars
1. Slawson, Chad. “The O-GlcNAc Post-Translational Modification: New
Insights into Cellular Function”. KUMC Cancer Center, May 7, 2013.
2. Slawson, Chad. “The O-GlcNAc Post-Translational Modification: A Critical
Regulator of Cellular Function”. University of Kansas Biophysics Seminar,
Department of Physics and Astronomy, March 28, 2013.
3. Slawson, Chad. “O-GlcNAc Signaling Regulates Mitochondrial Function:
Implications for Alzheimer’s Disease”. KUMC Translational Research
Forum, March 22, 2013.
4. Slawson, Chad. “The O-GlcNAc Post-Translational Modification: A Critical
Regulator of Cellular Function”. Kansas State University, Department of
Diagnostic Medicine and Pathobiology, March 14, 2013.
5. Slawson, Chad. “O-GlcNAc Signaling Regulates Mitochondrial Function:
Implications for Alzheimer’s Disease.” KUMC Alzheimer’s Center
Research Colloquium, Dec. 14, 2012.
6. Slawson, Chad. “Novel Roles for O-GlcNAc Signaling in the Regulation of
Mitosis and Gene Transcription”. University of Lausanne, Switzerland,
Center for Integrative Genomics, Aug. 31, 2012.
7. Slawson, Chad. “The O-GlcNAc Post-Translational Modification is a Key
Regulator of Cellular Function”. University of Kansas School of Medicine,
Department of Pharmacology, Jan. 31, 2012.
8. Slawson, Chad. “Controlling Cell Growth and Development with Sugar!”
Northwest Missouri State University, Department of Chemistry, Nov. 17,
2011.
9. Slawson, Chad. “O-GlcNAc Signaling in Growth and Development”.
University of Kansas, Protein-Structure Core, Nov. 2, 2011.
10. Slawson, Chad. “O-GlcNAcylation: A New Way to Regulate Mitosis”.
Pittsburgh State University, Department of Chemistry, March 4, 2011.
11. Slawson, Chad and Hart, Gerald W. “Controlling M Phase Progression by
O-GlcNAcylation”. University of Kansas, Department of Biochemistry and
Molecular Biology, April 5, 2010.
284
12. Slawson, Chad; Wang, Zihao; Udeshi, Namrata; Compton, Philip;
Shabanowitz, Jeffery; Hunt, Donald F.; and Hart, Gerald. “Extensive
Crosstalk Between GlcNAcylation and Phosphorylation Regulates
Cytokinesis”. FASEB Summer Research Conference, July 29, 2009.
13. Slawson, Chad and Hart, Gerald W. “The Sweet Side of M Phase: The
Regulation of Mitosis by O-GlcNAc”. Texas A&M University, Department
of Biochemistry and Biophysics, February 9, 2009.
14. Slawson, Chad and Hart, Gerald W. “Dynamic Regulation of the Cell
Cycle by O-GlcNAc Post-translational Modifications”. University of Iowa,
Department Anatomy and Cell Biology, November 24, 2008.
15. Slawson, Chad and Hart, Gerald W. “The Emerging Role of O-GlcNAc in
Mitotic Regulation”. 2008 Society for Glycobiology Annual Meeting,
November 13, 2008.
16. Slawson, Chad and Hart, Gerald W. “O-GlcNAc Transferase and OGlcNAcase Form a Mitotic Complex with Aurora Kinase B at M Phase to
Regulate the Post-translational Modifications of the Intermediate Filament
Protein Vimentin”. National Institute of Health Glycoscience Day,
Bethesda, MD. May 9, 2008.
17. Slawson, Chad and Hart, Gerald W. “Cell Cycle Regulation by O-GlcNAc
Post-translational
Modifications”.
Oklahoma
Medical
Research
Foundation, Program in Cell Cycle and Cancer Biology, February 27,
2008.
18. Slawson, Chad and Hart, Gerald W. “The Evolving Role of O-GlcNAc in
Cell Cycle Control and mTOR Signaling.” Colorado State University,
Department of Biochemistry And Molecular Biology, February 21, 2008.
19. Slawson, Chad and Hart, Gerald W. “Regulation of the Cell Cycle and
mTOR Signaling by O-GlcNAcylation.” University of Memphis, Department
of Biology, February 7, 2008.
20. Slawson, Chad and Hart, Gerald W. “New Frontiers in Protein Regulation:
The Role of O-GlcNAc in Cell Cycle Control and mTOR Signaling.”
University of Missouri, Department of Biochemistry, Jan. 29, 2008.
21. Slawson, Chad and Gerald Hart. “A New Look at Growth and Proliferation:
The Role of O-GlcNAc in Cell Cycle Control and mTOR Signaling.”
Virginia Commonwealth University School of Medicine, Jan. 17, 2008.
22. Slawson, Chad and Hart, Gerald W. “The Emerging Role O-GlcNAc in Cell
Cycle Control and mTOR Signaling.” University of Akron, Department of
Chemistry, Dec. 3, 2007.
285
23. Slawson, Chad and Hart, Gerald W. “Vimentin is a Target of an OGlcNAc/O-Phosphate Signaling Complex at M phase.” 2007 American
Society for Biochemistry and Molecular Biology Meeting, Washington D.C.
April 30, 2007.
24. Slawson, Chad and Hart, Gerald W. “Dynamic Interplay Between OGlcNAc and O-Phosphate: Implications for Cancer, Diabetes, and
Cardiovascular Disease.” Opening Lecture of the 17th Annual University of
Alabama Birmingham Vascular Biology and Hypertension Symposium,
Birmingham, AL. September 20, 2006.
25. Slawson, Chad; Pidala, Joseph; and Potter, Robert. “Decreases in Nacetylglucosamine Modified Proteins Correlate with Increased N-acetyl-βglucosaminidase Activity in Primary Breast Tumors.” 2000 Suncoast
Biomolecular Science Conference, Tampa, FL. October 15, 2000.
26. Slawson, Chad and Potter, Robert. “Teaching the Undergraduate Science
Laboratory.” Graduate Teaching Workshop, Center for Teaching
Enhancement, University of South Florida, Tampa, FL. August 2000.
27. Slawson, Chad and Potter, Robert. “Human Breast Tumors Show
Increased Cytoplasmic Protein Glycosylation,” 1998 Suncoast
Biomolecular Science Conference, Tampa, FL. October 9, 1998.
28. Slawson, Chad and Potter, Robert. “N-acetylglucosamine, Novel
Cytoplasmic Sugar Modification at Potential Phosphorylation Sites:
Proliferative Induced Changes in Modification.” Moffitt Breast Cancer
Research Conference, Tampa, FL. July 23, 1996.
Poster Presentations
1. Antonio Artigues, Ee Phie Tan, Maria Villar, J. Lu, Eva Selfridge, Russell
Swerdlow, and Chad Slawson. O-GlcNAc Signaling Regulates
Mitochondrial Function: Implications for Alzheimer’s Disease. ASMBM
Special Symposia, Post-Translational Modifications: Detection and
Physiological Role, October 11-14, 2012
2. Ee Phie Tan, Anish Potnis, and Chad Slawson. The Regulation of Aurora
Kinase B by O-GlcNAcylation. Experimental Biology Meeting, April 21,
2012.
3. Melody Chambers and Chad Slawson. Identification of OGT Interacting
Proteins at M Phase. Experimental Biology Meeting, April 21, 2012. Won
best poster in the DNA Replication, Recombination, Repair thematic
session.
286
4. Stephen Whelan, Ee Phie Tan, Catherine Costello, Mark McComb, and
Chad Slawson. Quantitative Proteomic Analysis of Proteins and PostTranslational Modifications During the Metaphase-Anaphase Transition
after Altered O-GlcNAcylation. Experimental Biology Meeting, April 21,
2012
5. Xiaofei Gao, Rachel Olsen, Chad Slawson, Philip R. Hardwidge. A
bacterial virulence factor targets TRAF proteins to impair the host immune
response. Harnessing Immunity to Prevent & Treat Disease Cold Spring
Harbor Meeting 2011
6. Ramirez-Correa, Genraro; Slawson, Chad; Gong, Weidong: Hart, Gerald
W.; and Murphy, Anne M. Increased Cardiac O-GlcNAc Transferase and
O-GlcNAcase Association to Actin, Tropomysin and MLC-1in Diabetes: A
Mechanism for O-GlcNAc Mediated Myofilament Calcium Desensitization.
Annual Biophysics Society Meeting, March 7, 2011.
7. Slawson, Chad; Wang, Zihao; Udeshi, Namrata; Compton, Philip;
Shabanowitz, Jeffery; Hunt, Donald F.; and Hart, Gerald. “Extensive
Crosstalk Between GlcNAcylation and Phosphorylation Regulates
Cytokinesis”. FASEB Summer Research Conference, July 29, 2009.
8. Slawson, Chad; Wang, Zihao; Udeshi, Namrata; Compton, Philip; Shabanowitz, Jeffery;
Hunt, Donald F.; and Hart, Gerald. “Identification and Quantification of Phosphorylated
and O-GlcNAcylated Proteins Associated with Mitotic Spindles and Midbodies during
Cytokinesis”. 2009 American Society for Mass Spectrometry Annual Meeting, June 2,
2009.
9. Slawson, Chad and Hart, Gerald. “The Emerging Role of O-GlcNAc in Mitotic
Regulation”. 2008 Society for Glycobiology Annual Meeting, November 13, 2008.
10. Slawson, Chad and Gerald Hart. “O-GlcNAc Transferase and O-GlcNAcase Form a
Mitotic Complex with Aurora Kinase B at M Phase to Regulate the Post-translational
Modifications of the Intermediate Filament Protein Vimentin”. National Institute of Health
Glycoscience Day, Bethesda, MD. May 9, 2008.
11. Slawson, Chad and Hart, Gerald W. “Vimentin is a Target of an O-GlcNAc/O-Phosphate
Signaling Complex at M phase.” 2007 American Society for Biochemistry and Molecular
Biology Meeting, Washington D.C. April 30, 2007.
12. Slawson, Chad and Hart, Gerald W. “O-GlcNAc Transferase is a Critical Regulator of
Cytokinesis: 2006 American Society for Biochemistry and Molecular Biology Meeting,
San Francisco, CA. April 1, 2006.
13. Slawson, Chad; Zachara, Natasha; Cheung, Win D.; Vosseller, Keith.; Lane, Malcolm D.;
and Hart, Gerald W. “Dynamic Regulation of O-GlcNAc During the Mammalian Cell Cyle”
2004 American Society for Biochemistry and Molecular Biology Meeting, Boston, MA.
June 17, 2004.
287
14. Slawson, Chad; Whelan, Stephen; and Potter, Robert. “Relationship Between Nuclear
and Cytoplasmic Protein O-Glycosylation and Mitogen Activated Protein Kinase Activity
in Breast Cancer Cell Lines.” 2001 American Society for Biochemistry and Molecular
Biology Meeting, Orlando, FL. April 3, 2001.
15. Slawson, Chad; Pidala, Joseph; and Potter, Robert.
“Increased N-Acetyl-βglucosaminidase Activity in Primary Breast Tumors Corresponds to a Decrease in
Serine/Threonine N-Acetylglucosamine Modified Proteins.” 2000 American Society for
Cell Biology Meeting, San Francisco, CA. December 9, 2000.
16. Slawson, Chad; Pidala, Joseph; and Potter, Robert.
“Increased NAcetylglucosaminidase Activity in Breast Tumor Tissue.” 2000 Annual American
Chemical Society Meeting Florida Chapter, Orlando, FL. May 13, 2000.
17. Slawson, Chad; Beakey, Paul; and Potter, Robert. “Novel Cytoplasmic Sugar
Modification at Potential Protein Phosphorylation Sites: Proliferative Induced Changes in
N-acetylglucosamine Modification.” 1996 Joint Annual American Chemical Society
Meeting, Orlando, FL. August 26, 1996.
18. Slawson, Chad; Beakey, Paul; and Potter, Robert. “Proliferative Induced Changes in the
Level of N-acetylglucosamine Protein Modification.” 1996 Suncoast Biomolecular Science
Conference, Tampa, FL. November 4, 1996.
Student Presentations
1. Zhen Zhang, Ee Phie Tan, Anish Potnis, Nathan Bushue, Flavia Costa, Kenneth
Peterson, and Chad Slawson. “O-GlcNAcylation Regulates γ-Globin
Transcription”. ASMBM Special Symposia, Post-Translational Modifications:
Detection and Physiological Role, October 11-14, 2012
Teaching Experience
• Lecturer, University of Kansas School of Medicine, Biochemistry, BCHM 922, October 1524, 2012
• Co-director, University of Kansas School of Medicine, Biochemistry 862, August 2012 present
• Lecturer, University of Kansas School of Medicine, Cell Communication: Receptor Tyrosine
Kinases, March 2012 - present.
• Lecturer, University of Kansas School of Medicine, Foundations of Medicine, Apoptosis
Lecture, 2011-present; Cell Cycle Lecture, 2011 - present; and Mitosis & Meiosis Lecture,
2011- present
• Small Group Discussion Leader, University of Kansas School of Medicine, Foundations of
Medicine, Sickle Cell Disease, 2011 - present; Mitochondrial DNA & Disease, 2011 –
present, and Huntington’s Disease, 2012 - present
• Lecturer, University of Kansas School of Medicine, Pre-matriculation Course, Cell Cycle
Lecture, 2011 - present
• Instructor, University of South Florida, Biochemistry Laboratory, 1996 – 2002
288
• Instructor, University of South Florida, General Chemistry Laboratory, 1994-1996
Mentoring
Current Students
•
•
Ee Phie Tan, 1st year Interdisciplinary Graduate Program Biomedical Science
Student, University of Kansas School of Medicine, Project: O-GlcNAcylation
Regulates Mitoic Spindle Function, 2012-present
Zhen Zhang, 2nd year Interdisciplinary Graduate Program Biomedical Science
Graduate Student, University of Kansas School of Medicine, Project:
Transcriptional Regulation by O-GlcNAc, 2012-present
Past Students
• Chris Lanza, Undergraduate Researcher from Santa Cruz University, University of Kansas
School of Medicine, Project: Spindle Function is Regulated by O-GlcNAcase, 2012
• Melody Chambers, Undergraduate Researcher from Rockhurst University, University of
Kansas School of Medicine, Project: Determining the OGT Mitotic Interactome, 2011
• Anish Potnis, Undergraduate Researcher from Georgia Institute of Technology, University
of Kansas School of Medicine, Project: The Role of O-GlcNAcylation in Regulating the
Aurora Kinase B Phosphorylation Circuit, 2011 and 2012
• Olof Lagerlof, Visiting Medical Student from the University of Gothenburg Sweden, Johns
Hopkins Medical Institute, Project: The Effects on Neuronal Cell Differentiation by OGlcNAc, 2007
• Luna Alamar, Biochemistry, Cellular, and Molecular Biology Ph.D. Program Laboratory
Rotation, Johns Hopkins Medical Institute, Project: O-GlcNAcylation of Protein RB, 2007
• John Bullen, Biochemistry, Cellular, and Molecular Biology Ph.D. Program Laboratory
Rotation, Johns Hopkins Medical Institute, Project: The Role of O-GlcNAc on Glut-4 Vesicle
Transport, 2006
• Jeremy Rotty, Biochemistry, Cellular, and Molecular Biology Ph.D. Program Laboratory
Rotation, Johns Hopkins Medical Institute, Project: TATA-Binding Protein is Modified by OGlcNAc, 2005
• Michael Housley, Biochemistry, Cellular, and Molecular Biology Ph.D. Program Laboratory
Rotation, Johns Hopkins Medical Institute, Project: O-GlcNAcylation in Yeast, 2003
• Susan Shafii, Honors Chemistry, University of South Florida, Project: O-GlcNAcase Activity
in Progesterone Stimulated Xenopus laevis Oocytes, 2002
• James Amburgey, Honors Chemistry, University of South Florida, Project: Characterization
of O-GlcNAcylation During Xenopus laevis Oocyte Maturation, 2001
• Joseph Padila, Honors Chemistry, University of South Florida, Project: Increased NAcetylglucosaminidase Activity in Human Breast Tumors, 2000
• Pedro Troya, Honors Chemistry, University of South Florida, Project: Characterization of OGlcNAc Modified Proteins in Human Breast Tumor Tissue, 2000
• Timothy Ragan, Honors Chemistry, University of South Florida, Project: Novel Partial
Purification of Soluble Monoglycosylated Proteins, 1999
• Javier Lopez, High School Math and Science Camp Individual Honors, University of South
Florida, Project: Measurement of O-GlcNAc in Xenopus laevis Oocyte Stages, 1998
• Fay Yao, High School Math and Science Camp Individual Honors, University of South
Florida, Project: Measurement of O-GlcNAc in Xenopus laevis Oocyte Stages, 1998
289
• Chakree Tanjaroon, Honors Chemistry, University of South Florida, Project: Detection of
Protein Glycosylation in the Cytoplasm and Nucleoplasm of Xenopus laevis Oocytes, 1998
• William Munroe, Honors Chemistry, University of South Florida, Project: Investigating the
Role of Nucleoside Diphosphate Kinase in Xenopus laevis Oocyte Maturation, 1998
• Parul Khator, High School Math and Science Camp Individual Honors, University of South
Florida, Project: Measurement of O-GlcNAc in Stage 6 Xenopus laevis Oocytes, 1997
Student Committees
•
•
•
•
Tanja Bhuiyan, Dissertation Committee, Dr. Winship Herr Laboratory, Center for
Integrative Genomics, University of Lausanne, Switzerland, Spring 2012 present
Allen Chazelle, Dissertation Committee, Dr. Kenneth Peterson Laboratory,
University of Kansas School of Medicine, Spring 2012 – present
Eva Selfridge, Dissertation Committee, Dr. Russell Swerdlow Laboratory,
University of Kansas School of Medicine, Fall 2011- present
Jason Barnett, Orals Committee, Dr. Aron Fenton Laboratory, University of
Kansas School of Medicine, Fall 2011
Service
•
•
•
Academic and Professionalism Committee: Students Promotions and Special
Programs Sub-committee, University of Kansas School of Medicine, Fall 2012 –
present
Medical Student Interviews, University of Kansas School of Medicine, Fall 2012 present
Heartland Undergraduate Biochemistry Forum Committee, University of Kansas
School of Medicine, Spring 2011 – present
Scientific Service
•
•
•
Session Chair, PTM Identification Methodology and Tools: ASMBM Special
Symposia, Post-Translational Modifications: Detection and Physiological Role,
October 11-14, 2012
Ad Hoc reviewer for the Journal of Proteomics Research 2012 - present
Ad Hoc reviewer for the Journal of Biological Chemistry 2011 - present
290
Date: 11/15/2013
291
I.
PERSONAL DATA
First Name:
Jinxi
Last name:
Wang
Professor (tenured)
Current Academic Rank:
Department(s):
Orthopedic Surgery (Primary), Biochemistry and Molecular Biology (Joint appointment)
Office Address:
3901 Rainbow Boulevard, Smith East 4010A, Mail-Stop 3017, Kansas City, KS 66160
Phone:
913-588-0870
Fax:
913-495-7773
Email:
[email protected]
Years
Degree
Institution
1987
M.D. (Medicine)
Suzhou Medical College, Suzhou, China
1991
Ph.D. (Cell Biology)
Suzhou Medical College and Loma Linda
University, Loma Linda, CA
Years (Inclusive)
Degree
Institution
1987-1992
Orthopaedic Residency
Suzhou Medical College Hospital
1992-1995
Advanced Coursework in Bone Biology
Harvard Medical School, Boston, MA
1992-1996
Research Fellow in Molecular and
Cellular Biology of Skeletal Tissues
Harvard Medical School and
Children’s Hospital, Boston, MA
Month and Year
Position
Institution
July/1996 - Dec/2004
Instructor in Orthopaedics
Harvard Medical School, Boston, MA
July/1996 - Dec/2004
Staff Scientist, Department of Orthopaedic
Surgery
Children’s Hospital, Boston, MA
Dec/2004 - June/2012
Associate Professor of Orthopedic Surgery
(primary appointment)
University of Kansas, School of
Jan/2006 – June/2012
Associate Professor of Biochemistry & Molecular
Biology (secondary appointment)
Dec/2004 - present
Director, The Harrington Laboratory for Molecular
Orthopedics
Nov/2011 - present
Mary A. & Paul R. Harrington Distinguished
Professor in Molecular Orthopedics
292
July/2012 - present
Professor of Orthopedic Surgery (primary) and
Biochemistry & Molecular Biology (secondary)
Professional Registration/Licensure N/A (I am not practicing medicine)
Year
Number
State
N/A
Professional Certification(s) N/A
Date
Board
N/A
Date
1995- present
Member, American Society for Bone and Mineral Research
1996- present
Member, International Bone and Mineral Society
2002- present
Member, International Association for Biological & Medical Research
(IABMR)
2002- 2012
Board Director (for development and membership) of IABMR
2003- present
Active Member, Orthopedic Research Society (U.S.A.)
2007- 2008
President-Elect, Sigma Xi Scientific Research Society KUMC Chapter
2008- 2009
President, Sigma Xi Scientific Research Society KUMC Chapter
2009-2010
Past-Present, Sigma Xi Scientific Research Society KUMC Chapter
2012-present
Member, Osteoarthritis Research Society International
Year
Award
1991
Young Investigator Research Award, granted by the Department of Public
Health, Jiangsu Province, R. R. China
1994
Research Award for Outstanding Research Achievement in
Pathogenesis of Osteoarthritis Following Osteonecrosis of the
Femoral Head, granted by the Ministry of Health, P. R. China
1998
Young Investigator Award, granted by the Executive Board of the 6th
International Conference on Chemistry and Biology of Mineralized
Tissues, Vittel, France
National Institutes of Health (NIH, U.S.A.) Research Service Award
1998
2011
The Mary A. & Paul R. Harrington, MD, Distinguished Professorship
in Molecular Orthopedics
293
II. TEACHING ACTIVITIES
(Teaching evaluations and other evidence of quality teaching must be attached; this represents the teacher’s portfolio
and should be accurately summarize ALL of your teaching activities.)
I thoroughly enjoy my opportunities to teach and participate in the training of learners in musculoskeletal biology
and diseases. I have been teaching medical, dental and graduate students, orthopedic residents, and postdoctoral
research fellows since 1996 in the following areas: (a) bone and cartilage biology, (b) bone and cartilage
regeneration, (c) mechanisms for mineralization of bone and teeth, and (d) specific skeletal diseases such as
osteonecrosis and osteoarthritis.
During my own training and school courses, I found that teachers who focused on problem solving skills and
utilized multiple sources, such as textbooks, additional materials, and personal experiences, stimulated my
intellectual curiosity and ability to function as a lifetime learner. In my lectures and laboratory teaching, I use
heuristic educational styles to encourage my students, postdoctoral fellows, and residents not only to memorize
the basic knowledge from textbooks and lectures, but also to be creative, innovative problem solvers. I try to
stimulate higher learning beyond textbooks. I also use various methods and vary my expectations for different
learners at bachelor, graduate, and postgraduate levels to meet their specific needs.
At KU School of Medicine, one of my major teaching activities has been focused on teaching orthopedic
residents in the area of orthopedic basic sciences with a focus of skeletal biology. My M.D., Ph.D., and
orthopedic residency training background enables me to effectively integrate orthopedic basic sciences with
clinical application through both formal lectures and group discussion (e.g. Journal Club which is focused on
publications from the Journal of Bone & Joint Surgery and other orthopedic journals). As an instructor for the
Foundations of Medicine and Musculoskeletal Modules at KU School of Medicine, I have presented Bone &
st
Cartilage Histopathology to 1 year medical students and Bone
nd
& Joint Histopathology to 2
year medical students, respectively.
In addition, I teach histology, cell biology, biochemistry, and molecular biology of skeletal tissues, as well as
manuscript and grant writing, to pre-medical students, medical and graduate students, postdoctoral fellows, and
orthopedic residents through daily research activity in the orthopedic research laboratory.
Instruction:
Academic
Year
20012004
Instruction
Course
Oral Biology
604B
Title
Type
Hours
Student
No
Type
New trends in
mineralized tissue
Lecture
12/year
25-30
Dental Students,
Harvard Dental
School
regeneration
2005Present
Orthopedic
Basic Sciences
Bone and cartilage
development
Lecture
3-6/year
15-20
KU Orthopedic
Residents
2005Present
Orthopedic
Basic Sciences
Basic biology of
skeletal cells
Same
3-6/year
15-20
KU Orthopedic
Residents
2005Present
Orthopedic
Basic Sciences
Bone modeling and
remodeling
Same
3-6/year
15-20
KU Orthopedic
Residents
294
2005Present
Orthopedic
Basic Sciences
Function of the growth
plate
Same
3-6/year
15-20
KU Orthopedic
Residents
2005Present
Orthopedic
Basic Sciences
Cartilage composition
& metabolism
Same
3-6/year
15-20
KU Orthopedic
Residents
2005Present
Orthopedic
Basic Sciences
Articular cartilage
repair
Same
3-6/year
15-20
KU Orthopedic
Residents
2005Present
Orthopedic
Basic Sciences
Osteoarthritis (etiology
& biochemistry)
Same
3-6/year
15-20
KU Orthopedic
Residents
2005Present
Orthopedic
Basic Sciences
Fracture healing
Same
3-6/year
15-20
KU Orthopedic
Residents
2005Present
Orthopedic
Basic Sciences
Bone defect repair
Same
3-6/year
15-20
KU Orthopedic
Residents
2005Present
Orthopedic
Basic Sciences
Basics of molecular
biology
Same
3-6/year
15-20
KU Orthopedic
Residents
2005Present
Orthopedic
Basic Sciences
Bone biology lab
techniques
Same
3-6/year
15-20
KU Orthopedic
Residents
2011-
Foundations of
Medicine
Module
Bone & Cartilage
Histopathology
Presentation
3/year
25
1 year medical
students, KU School
of Medicine
2011-
Musculoskeletal
Module
Bone & Joint
Histopathology
Presentation
3/year
25
2 year medical
students, KU School
of Medicine
2012-
Advanced
Rehabilitation
Sciences
Structure of synovial
joints and osteoarthritis
Presentation
3/year
3
Graduate students,
KU School of Health
Professions
st
nd
Instruction
Academic
Year
Course
1996-2004
Bone Histology
Microstructure
of mouse joints
1999-2004
Bone Histology
2005-2006
Title
Student
Hours
No
Lab discussion
groups
60/year
1 - 10
Harvard graduate
and med students
Microstructure
of cortical bone
Lab discussion
groups
30/year
1-6
Harvard graduate
and med students
Bone Biology
Bone
mineralization
Research lab
training
80/year
1
Andy Anderson, KU
medical student
2006-2007
Bone Biology
Bone
regeneration
Research lab
training
80/year
1
John Yost, KU Premedical student
2008
Bone Biology
Bone
regeneration
Research lab
training
10/week
1
John Yost, KU
medical student
(May-July)
Type
Type
Cartilage
Biology
Osteoarthritis
Research lab
training
10/week
1
M. Kareem Shaath,
KU Pre-medical
student
2009-2010
Cartilage
Biology
Osteoarthritis
Research lab
training
10/week
1
Brian Gardner, KU
premedical student
2010
(May-July)
Cartilage
Biology
Osteoarthritis
Research lab
training
10/week
1
M. Kareem Shaath,
KU medical student
2010-2011
Cartilage
Biology
Human
osteoarthritis
Research lab
training
10/week
1
James Bernard, KU
premedical student
2011
(May-July)
Cartilage
Biology
chondrocyte
differentiation
Research lab
training
10/week
1
Clayton Theleman
KU medical student
2011
(May-July)
Bone Biology
BSP in
osteogenesis
Research lab
training
10/week
1
John Garlish, KU
medical student
2007Present
Journal Club
Clinical & basic
orthopedics
Discussion groups
8/year
15-20
KU orthopedic
residents
2009
(May-July)
Clinical: N/A
Student
Year
Hours
No
Length of Service
Type
N/A
Year
1996-1999
1998-2000
2000-2002
2001-2003
2001-2003
Student Name
Thesis Title
Degree
(Completed/In process)
John C. Huang
Protein kinases and physpho- proteins in
developing enamel
Doctor of Medical Sciences
Harvard School of Dental
Medicine - Completed
Susan So
Osteopontin and bone sialoprotein as a
function of turkey tendon mineralization
Master in Medical Sciences
Margo T. Kusienski
Immunohistological and biochemical
studies on dentin phosphoproteins
Joseph Hung
The efficacy of alloplastic bone implants
on the healing of rat calvarial defects
Robert J. Bruzzichesi
Characterization of extracellular matrix
phosphoproteins of cementum
296
2013-
Nathan Wilson
2013-
Sushma
Jadalannagari
SPECC1L modulation of adherens
junctions for neural crest cell function
during craniofacial morphogenesis
Decellularized Wharton’s Jelly Matrix to
accelerate bone Healing in a murine
calvarial bone defect model
PhD in Cell Biology
School of Medicine – In
process
PhD in Biomaterials and
Tissue Engineering
Supervision of Postdoctoral Fellows/Residents
Year
Fellow/Resident Name
Area of Study
2001-2002
Jun Yan, M.D.
Osteonecrosis and osteoarthritis
2002-2005
Jochen Hofstaetter, M.D.
Osteonecrosis and osteoarthritis
2002-2004
Lan Xu, Ph.D.
In vivo function of bone sialoprotein
2006-2007
Rama Garimella, Ph.D.
Cellular and molecular biology of skeletal tissues
2006-2010
Marianna Rodova, Ph.D.
Cellular and molecular biology of skeletal tissues
2008-2010
Brent Woodbury, M.D.
(Orthopedic Resident)
Molecular mechanisms of osteoarthritis
2009-2010
J. Paul Schroeppel, M.D.
Articular cartilage biology and its repair
2010-2013
William Kramer, M.D.
Mechanisms of post-traumatic osteoarthritis
2011-
Kenneth Caldwell, M.D.
Articular cartilage repair
2011-
Mingcai Zhang, Ph.D.
Bone sialoprotein and osteogenesis
2012-
Yi Feng, Ph.D.
NFAT1 deficiency and osteoarthritis
2013-
Paul Cowan, M.D.
Biomaterials in cranial bone repair
Advising (Thesis or dissertation defense committees: student academic group/individual)
Date
John C. Huang’s dissertation
Ph.D. Student, Harvard School of Dental Medicine
04/23/1999
committee
04/05/2000
Susan So’s thesis committee
Master Student, Harvard School of Dental Medicine
05/02/2002
Dasha Ellezian’s thesis committee
Master Student, Harvard School of Dental Medicine
Nathan Wilson’s dissertation
TBD
committee
PhD Student, University of Kansas School of
Medicine
TBD
Sushma Jadalannagari’s dissertation
committee
PhD Student, University of Kansas School of
Engineering
297
Date
Title
Place
Teaching Function
June-July/2010
Bone biochemistry and
molecular biology
Harrington Laboratory for
Molecular Orthopedics, KUMC
Supervised Armand Heyns,
post-baccalaureate intern
Development of Educational Materials
(Course materials e.g. syllabi, educational software packages, web sites, films, educational tapes and
evaluation tools)
In addition to the development of PowerPoint and handout teaching materials for orthopedic resident
lectures, I have developed a number of laboratory protocols to help students and fellows/residents
understand basic principles and practical experimental procedures. Most of these procedures are
developed for my specific
projects and are technically difficult. My protocols have made these complex procedures easy to understand
and follow, which have significantly shortened students/trainees’ learning time and improved their research
performance. These protocols are listed below.
Year
Title Description
Intended Audience
2006
Purification of bone sialoprotein from bovine bones
Pre-med, med/grad students,
research fellow, residents
2007
Isolation of RNA from rodent articular cartilage tissue
Same
2008
RNA/DNA isolation from human cartilage tissue
Same
2009
RNA isolation from cultured articular chondrocytes
Same
2010
Purification of bone sialoprotein from rat bones
Same
Educational Leadership
(Responsibility for courses and other leadership activities including mentoring of junior faculty.
Please list faculty members mentored)
I joined the faculty of Orthopedic Surgery in 2004. My major mission was to establish and develop a new
bone biology laboratory for both research and education. As a lab director, I have developed and led a
new biology laboratory named “The Harrington Laboratory for Molecular Orthopedics.” This laboratory is
well organized and equipped with instruments not only for scientific research but also for education,
such as micro-dissection and presentation of bone and cartilage cells, bone microstructure, function of
various skeletal cells, and histopathology of skeletal tissues. This educational lab setting has provided a
learning environment to greatly help medical students and orthopedic residents better understand the
morphology and cellular/molecular biology of the skeletal system, which is an important part of their
required examinations. For example, Step 1 of USMLE (for medical students) and Orthopedic InTraining Examination (OITE, for orthopedic residents) contain basic sciences of the skeletal system.
At the national level, I have been serving as a member of the New Investigator Mentoring Committee of
the Orthopedic Research Society (ORS, USA) since February of 2012 to assist junior faculty members
and new scientists with grant writing and career development. I have pre-reviewed grant applications
from junior faculty members at other universities and provided my comments and suggestions to help
298
them improve their proposals prior to official submission. In addition, I have given lectures at the Grant
Writing Workshops sponsored by ORS.
III. SERVICE ACTIVITIES
Professional Service: N/A
The diverse area of professional service includes patient care. Applicants should select
measures that most clearly and concisely document their accomplishments and the value of these
activities to the Medical School and University. Measures of both quantity and quality of activities are
required and if necessary, applicants should provide brief descriptions to assist reviewers.
Measures of patient care activities include numbers of patients, time allocation in clinical
activity, procedures completed, Relative Value Units (RVUs), and value to the School of the
clinical service. If the primary quality evaluation is the subjective assessment of peers, this should
be available in letters from departmental colleagues, chair, or referees.
The significance of professional service in the forms of task forces, committees and similar groups
should be explained and the specific role of the applicant clarified.
Professional consulting services must have academic credibility and clear service intent and not be
performed primarily for personal profit.
Academic Service:
In academic service the contribution of the candidate to the academic community should be
clearly documented. Names and dates of committees, task forces, or working groups should be
provided. A concise description of the significance of the group and explanation of the role of the
applicant should be provided.
Activities related to Academic Societies should be documented in this section.
Intramural service.
(A) I have served on the Research Committee in the Orthopedic Surgery Department at KUMC to
assist the Committee with organization and supervision of student and resident research since
2005.
(B) I served on the KUMC Biomedical Research Training Grant Applications Review Committee and
reviewed grant applications from postdoctoral fellows and pre-doctoral students in 2007, 2008, and
2010.
(c) I have served as a regular voting member of the KUMC Institutional Animal Care and Use
Committee (IACUC), representing the Surgery Departments, since February 1, 2011 and will continue this
service until January 31, 2014. As an IACUC Committee member, I am responsible for reviewing animal
protocols, addenda, and standard of animal procedures. Also, I am required to attend IACUS meetings
and inspect animal facilities.
299
Extramural service.
(A) Committee member/officer of professional organizations
2002-2012
Board Director for development and membership of International Association for
Biological and Medical Research (IABMR)
2007-2008
President-Elect of Sigma Xi Research Society KUMC Chapter (Sigma Xi is an
international scientific research society with more than 60,000 active
members)
2008 - 2009 President of Sigma Xi Research Society KUMC Chapter
2012Member, the New Investigator Mentoring Committee, Orthopedic Research Society
(ORS, USA)
(B) Conference organizing committee/session chair
2007 - 2009 Member of Organizing Committee, Sigma Xi KUMC Chapter monthly seminar
May/2008
Program Chair, Sigma Xi KUMC Chapter Annual Symposium
Aug/2012
Organizing Committee Member and Scientific Advisor, 2012 International
Conference on Orthopedics and Rheumatology, August 13-15, 2012, Chicago,
IL
Aug/2012
Session Chair: Regenerative and Molecular Orthopedics Session, 2012 International
Conference on Orthopedics and Rheumatology, August 13-15, 2012, Chicago, IL
Jan/2013
Session Moderator: New Investigator Recognition Award Session 2 – Bone.
ORS 2013 Annual Meeting, San Antonio, TX, USA, January 26-29, 2013.
Oct/2013
Session Moderator: Oral Poster Presentations - Late-Breaking
ASBMR 2013 Annual Meeting, Baltimore, MD, USA, October 4-7, 2013.
(C) Abstract reviewer
2008
Reviewed 120 Abstracts in the area of “Disorders of Bone and Mineral Metabolism” for
the ASBMR (the American Society for Bone and Mineral Research) 30th Annual Meeting,
Montreal, Canada, September 12-16, 2008.
2009
Reviewed 94 abstracts in the same area mentioned above for the ASBMR 31st Annual
Meeting, Denver, CO, USA, September 11-15, 2009.
2011
Reviewed 51 abstracts in the areas of cartilage, synovium, meniscus, and osteoarthritis
for the ORS 2012 Annual Meeting.
(D) Manuscript reviewer for the following
journals/books: 1997 - Journal of Cellular
Engineering
300
2006 - Biomaterials, Biochemical Journal
2008 - A book chapter entitled “DNA Methylation and Osteoarthritis” in the Book Epigenetics of Aging
2009 - Acta Biomaterialia, Journal of Orthopaedic Research
2010 - Journal of Bone and Mineral Research, Calcified Tissue International
2012 - Arthritis & Rheumatism, Molecular Endocrinology, Journal of Orthopaedic Surgery and Research
2013 - Arthritis Research and Therapy
(E) Editorial Board
2011 - International Journal of Clinical and Experimental Medicine (Editor for bone
research) 2011 - Scientific World Journal (Editor for biomaterials and skeletal tissue
repair)
2011 - Frontiers in Craniofacial Biology
2012 - Journal of Bone and Mineral Research (JBMR)
(F) Grant Reviewer
2011- National Institutes of Health (NIH): Ad Hoc Member of NIH Skeletal Biology
Structure and Regeneration (SBSR) Study Section.
2012- Israel National Science Foundation: reviewer for grant applications in skeletal biology and
diseases. 2012- Netherlands Organization for Health Research and Development, Independent
Grant Reviewer 2012- NIH: Ad Hoc Member of Special Emphasis Panel, Skeletal Pathology and
Orthopedics
2012- US Department of Defense (DoD), Peer Reviewed Medical Research Program (PRMRP), Member
of the Peer Review Panel for Investigator Initiated Research Award (IIRA) in the topic area of
post-traumatic osteoarthritis.
2013- NIH: Ad Hoc Member of NIH Skeletal Biology Development and Disease (SBDD) Study
Section. 2013- NIH: Ad Hoc Reviewer for the NIH Director’s Early Independence Awards (DP5
grants).
IV. RESEARCH and SCHOLARLY ACTIVITIES:
1. Bone sialoprotein and bone regeneration. Bone sialoprotein (BSP) is one of the major noncollagenous extracellular matrix (ECM) proteins in bone and tooth. BSP is highly expressed in
developing and postnatal regenerating bone, but its precise function is largely unclear. My
research group was the first to report that implantation of BSP with reconstituted type-I collagen
stimulated osteoblast differentiation and bone formation during the repair of cranial bone
defects in rodents. In contrast, no bone formation was observed when BSP-collagen was
implanted into the thoracic subcutaneous soft tissue. The osteogenic bioactivity of BSP in
stimulating bone regeneration is an unprecedented result; the mechanisms of tissue-specific BSP
action remain unclear. The major goal of our studies is to explore the molecular and cellular
301
mechanisms of tissue- specific BSP action in osteogenesis and its application for enhancing bone
regeneration. Successful completion of this project will significantly advance our understanding of
the mechanisms of BSP-specific biological activities, which will possibly lead to improved methods for
the treatment of bone defects in humans. Moreover, understanding the cell type- and bone
environment-dependent effects of BSP on osteogenesis may provide new insights into the longstanding question of why metastasis of BSP-expressing tumors is preferential to bone. This research
project is currently supported by an NIH/NIDCR grant (R01 DE018713, PI: Jinxi Wang).
2. Pathogenetic mechanisms and therapeutics for osteoarthritis. Osteoarthritis (OA) is the most common form
of joint disease. No proven pharmacologic therapy is currently available to prevent the initiation or reverse
the progression of OA, largely because the root causes of OA remain unclear. My research interest in OA
was initiated during the early studies for my Ph.D. thesis on the pathogenesis of secondary OA following
osteonecrosis of the femoral head in humans. Recently, my research group discovered that mice lacking
transcription factor NFAT1 exhibit normal skeletal development but display most of the characteristics of OA
seen in humans. The objective of this project is to explore the molecular and cellular mechanisms underlying
the pathogenesis of NFAT1 deficiency-associated OA in mice and possibly in humans. Successful
completion of this project may provide a better understanding of the pathogenesis of OA and identify target
cells of NFAT1 in the joint, thereby establishing a rationale for development of preventive and therapeutic
strategies using NFAT1 as a molecular target for NFAT1 deficiency-induced OA. This project is currently
supported by an NIH R01 grant (R01 AR059088, PI: Jinxi Wang).
3. Post-traumatic osteoarthritis. The objective of this project is to examine whether NFAT1 expression level
in joint tissues affects the time of onset and severity of post-traumatic knee OA after surgical transection
of the medial meniscotibial ligament in mice. This project is currently supported by a DoD grant (PI: Jinxi
Wang).
4. Regulatory mechanisms of chondrocyte differentiation and articular cartilage regeneration. Currently,
efforts to repair damaged articular cartilage face major obstacles due to limited intrinsic repair capacity of
the tissue. Animal and human studies have demonstrated that a full thickness defect of articular cartilage
penetrated through the subchondral bone to the bone marrow spaces can be repaired morphologically
through the proliferation and differentiation of bone marrow mesenchymal stem cells into cartilage
cells which synthesize a cartilage matrix or by implanting chondrocyte-seeded biomaterials with or without
growth factors using tissue engineering technology. However, the repaired articular cartilage tissue
degenerates with reduced expression levels of cartilage markers after several months, and the joints
with articular cartilage lesions eventually develop osteoarthritis. The objective of this project is to
investigate the regulatory mechanisms of chondrocyte differentiation and articular cartilage regeneration
in mice and humans, thereby developing more effective therapeutic strategies for the healing of articular
cartilage defects.
Through these projects, I have developed research expertise in the areas of bone cell differentiation and
bone regeneration, chondrocyte function and articular cartilage regeneration, and pathogenesis of
osteoarthritis.
1 Grants and contracts
(Information must include whether the nominee (name bolded) is the principal investigator or a coinvestigator, names of all investigators, title of grant, funding source, dollar amount in direct costs, and
years during which grant applies. Co- investigators must specify role). Provide the cover sheet, abstract
and Notice of Award in PDF for all grants or contracts awarded in last five years (submit online).
Previous Grants and contracts awarded: (List in chronological order – oldest first, newest last)
302
Principal
Investigator
Yaotang
Wu
Jinxi Wang
Melvin
Glimcher
Jinxi Wang
Investigators
Jinxi Wang
(Co-PI)
(in vivo tissue
analyses)
Evaluation of solid
state MRI of bone
mineral and matrix
Funding
Source
Direct
Costs
Orthopaedic
Research &
Education
Foundation
(OREF)
$150,000
$116,000
Melvin
Glimcher
(Sponsor)
In vivo molecular and
cellular responses to
osteogenic factors
NIH Research
Fellowship
Jinxi Wang
(Co-PI, animal
surgery and
histopathology)
Use of alendronate to
prevent osteoarthritis
following osteonecrosis of the femoral head
Arthritis
Foundation
Osteogenic potential
of bone sialoprotein
OREF
$100,000
Nature of bone
mineral: inception,
maturation, aging
NIH/NIA R01
AG014701
$2,986,000
Jinxi Wang
Melvin
Glimcher
Title of Grant
Jinxi Wang
(Co-I, in vivo
mineralization)
Yaotang Wu
(MRI analysis)
Mechanism of SiteSpecific Bone
Differentiation
Pathways
Jinxi Wang
NIH/NIAMS
Years
Status
1997-2000
Completed
1998-2001
Completed
2000-2003
Completed
2001-2004
Completed
1997-2008
Completed
2006-2009
Completed
$150,000
$150,000
R03AR052088
Current Grants and contracts awarded: (List in chronological order – oldest first, newest last)
Principal
Investigator
Jinxi Wang
Jinxi Wang
Investigators
Paul Trainor
(signaling
study)
Title of Grant
Bone Sialoprotein in
Osteogenesis and
Bone Regeneration
Gerry Carlson
(protein chem)
H.C. Anderson
(bone pathol)
Xiaobo Zhong
Transcription Factor
NFAT1 Deficiency
and Osteoarthritis
Funding
Source
NIH/NIDCR
Direct Costs
Years
Status
2010-2015
Active
2011-2016
Active
2012-2016
Active
$1,032,000
R01
DE018713
NIH/NIAMS
R01
$1,125,000
AR059088
(epigenetics)
Cory Berkland
(KU Lawrence)
Jinxi Wang
(PI on
Subcontract )
Jinxi Wang
(implantation
of biomaterials
and bone
healing
analyses)
$1, 035,000
Integrative Colloidal
Gels for Cranial
Defect Repair
303
NIH/NIDCR
R01
DE022472
Ken Brandt
and A. Ahad
Haleem,
(clinical
consultation)
Jinxi Wang
Deficiency of
Transcription Factor
NFAT1 and
Posttraumatic
Osteoarthritis
Department
of Defense
(DoD)
$750,000
W81XWH1210304
2012-2015
Active
Principal
Investigator
Xinmai Yang
2
Funding
Source
Investigators
Title of Grant
Jinxi Wang
(subcontract PI)
Mitigate
Osteoarthritis by
Shear Wave
Direct
Costs
Years
Status
$500,000
DoD
2014-2017
Pending
Full length, peer-reviewed articles in scientific journals: (Provide names of all authors, year, title,
journal, volume, and inclusive pages. The articles must be numbered in chronological order
(oldest publications first, most recent publications last). In cases where there are multiple
publications within a year, they should be listed by alphabetical order. The list should not include
papers "in preparation," "submitted," or "under revisions", nor should it include conference
proceedings, published abstracts, and book reviews (which need to be listed separately).
Articles published: (Provide a PDF of each peer-reviewed article (PRA) published within
99
01PRA - Wang J, Dong T, Tang T, Zu G. m-Tc-MDP bone scintigraphy for diagnosis of femoral head
necrosis after femoral neck fractures. Jiangsu Med J 1989; 15:194-196.
02PRA - Wang J, Xu L, Dong T. Arthoroscopic examination and surgery of the knee. Jiangsu Med J 1989;
15:579-581.
03PRA - Wang J, Dong T, Liu Z. A histological study of avascular necrosis and repair of the femoral head
after femoral neck fractures. Acta Acad Suzhou 1990; 10:263-267.
04PRA - Wang J, Dong T, Tang T. A combination of clinical and research training: A new orthopaedic
residency program. Chin J Orthop 1990; 10:308-309.
05PRA - Wang J, Dong T, Liu Z. Pathologic observations of osteoarthritis following avascular necrosis of the
femoral head. J Clin Exp Pathol 1991; 7:111-114.
06PRA - Wang J, Dong T, Liu Z. Correlations of intramedullary haemorrhage and avascular necrosis of
the femoral head. J Bone Joint Injury 1992; 7:7-10.
07PRA -
Sang S, Jian Y, Wang J. The scintigraphic changes in the repair process of experimentally
induced avascular necrosis of the femoral head. Chin J Nucl Med 1993; 13:102-104.
304
08PRA - Wang J, Tong T, Chen X, Dong Q, Wong W, Dai M, Chen M. A biomechanical study on the
repairing process of avascular necrosis of the femoral head in dogs. Chin J Surg 1993;
31:374-377.
09PRA - Dong QR, Wang JX, Dong TH. Early diagnosis by scintigraphy of segmental collapse of the
femur head following femur neck fracture. J Radiol 1994; 75:423-425.
10PRA - Wang J, Dong Q, Dong T, Wu Y, Sang S. The changes in radionuclide bone imaging during the
repair process of the human femoral head necrosis after femoral neck fracture. Chin J Orthop
1994; 14:751-754.
11PRA - Dong Q, Wang J, Dong T. Prediction of segmental collapse of femoral head after femoral neck
fracture by scintimetry. Clin J Surg 1994; 32:520-522.
12PRA - Dong Q, Wang JX, Dong T. Early diagnosis by scintigraphy of femur head necrosis after femur neck
fracture. Chirurgie 1995; 120:194-197.
13PRA -
Wang J, Dong T, Tang T, Zu G. Results of vascularized (muscle) pedicle bone grafts for
femoral head necrosis after femoral neck fracture: Three to nine-year follow-up in 87 cases.
Acta Acad Med Suzhou 1995; 1:102-109.
14PRA - Dong T, Wang J. Implantation of hydroxyapatite-methyl-methyacrylate in the treatment of avascular
necrosis of the femoral head: Experimental study and preliminary clinical application. Acta Acad
Med Suzhou 1995; 1:1-8.
15PRA - Dong Q, Wang J, Zheng Z, Dong T, He G. Early diagnosis for segmental collapse of the femoral
head after femoral neck fracture by scintimetry. Acta Acad Med Suzhou 1995; 1:117-121.
16PRA - Wang J, Glimcher MJ, Mah J, Zhou HY,Salih E. Expression of bone microsomal casein kinase II,
bone sialoprotein, and osteopontin during the repair of calvarial defects. Bone 1998; 22:621-628.
17PRA - Wu Y, Ackerman JL, Chesler DA, Li J, Neer RM, Wang J, Glimcher MJ. Evaluation of bone mineral
density using three dimensional solid state phosphorus-31 NMR projection imaging. Calcif Tissue
Int 1998; 62:512-518.
18PRA - Wang J, Kennedy JG, Kasser JR, Glimcher MJ, Salih E. Novel biological property of purified
native bone sialoprotein in bone repair of a calvarial defect. Orthop Trans 1999; 22:951-952.
19PRA - Wu Y, Chesler DA, Glimcher MJ, Garrido L, Wang J, Jiang HJ, Ackerman JL. Multinuclear solidstate three-dimensional MRI of bone and synthetic calcium phosphates. Proc Natl Acad Sci USA
1999; 96:1574-1578.
20PRA -
21PRA defects.
Wang J, Mah J, Zhou HY, Glimcher MJ, Salih E. Interrelationship Between bone microsomal
casein kinase II, bone sialoprotein, osteopontin and mineral accumulation. Orthop Trans 1999;
22:832-833.
Wang J, Glimcher MJ. Characterization of matrix-induced osteogenesis in rat calvarial bone
Part I: Differences in the cellular response to demineralized bone matrix implanted in calvarial
defects and n subcutaneous sites. Calcif Tissue Int 1999; 65:156-165.
305
22PRA defects.
Wang J, Glimcher MJ. Characterization of matrix-induced osteogenesis in rat calvarial bone
Part II: Origins of bone forming cells. Calcif Tissue Int 1999; 65:486-493.
23PRA - Ranger AM, Gerstenfeld LC, Wang J, Kon T, Bae H, Gravallese EM, Glimcher MJ, Glimcher LH.
The Nuclear factor of activated T cells (NFAT) transcription factor NFATp (NFATc2) is a
repressor of chondrogenesis. J Exp Med 2000; 191:9-21.
24PRA - Wang J. Spatial orientation of the microscopic elements of cortical repair bone. Clin Orthop 2000;
374:265-277.
25PRA - Wang J, Yang R, Gerstenfeld LC, Glimcher MJ. Characterization of matrix-induced osteogenesis
in rat calvarial bone defects. Part III: Gene and protein expression. Calcif Tissue Int 2000;
67:314-320.
26PRA -
Dong T, Liu S, Zhu G, Wei W, Wang J. Implantation of hydroxyapatite methylmethacrylate
complex in the prevention of degenerative osteoarthritis following osteonecrosis of the femoral
head. Chin J Orthop 2002; 22:84-87.
27PRA -
Salih E, Wang J (Co-first-author), Mah J, Fluckiger R. Natural variation in the extent of
phosphorylation of bone phosphoproteins as a function of in vivo new bone formation induced by
demineralized bone matrix in soft tissue and bony environments. Biochem J 2002; 364:465-474.
28PRA - Wang Y, Wang J, Xu Z, Wu S, Lu X, Li G. An experimental study of cranial bone repair
elicited by implantation of bone-healing herb extract. Chin J Orthop Traum 2003; 16(6):346-348.
29PRA - Wang J, Glimcher MJ. Spatial differences in the cellular response to demineralized bone matrix
implanted in long bone defects of rabbits. Connect Tissue Res 2003; 44(suppl. 1):365-366.
30PRA -
MacLean HE, Kim JI, Glimcher MJ, Wang J, Kronenberg HM, Glimcher LH. Absense of
transcription
factor c-maf causes abnormal terminal differentiation of hypertrophic
chondrocytes during endochondral bone development. Dev Biol 2003; 262:51-63.
31PRA - Mah J, Hung J, Wang J, Salih E. The efficacy of various alloplastic bone grafts on the healing of
rat calvarial defects. European J Orthodontics 2004; 26: 475-482.
32PRA - Wang J , Zhou HY, Salih E, Xu L, Wunderlich L, Gu X, Hofstaetter JG, Torres M, Glimcher MJ.
Site- specific In vivo calcification and osteogenesis stimulated by bone sialoprotein. Calcif Tissue
Int 2006; 79:179-189.
33PRA - Hofstaetter JG, Wang J, Yan J, Glimcher MJ. Changes in bone microarchitecture and bone mineral
density following experimental osteonecrosis of the hip in rabbits. Cells Tissues Organs 2006;
184:138-147.
34PRA -
Xu L, Anderson AL, Q Lu, Wang J (corresponding author). Role of fibrillar structure of
collagenous carrier in bone sialoprotein-mediated matrix mineralization and osteoblast
differentiation. Biomaterials 2007; 28:750-761.
306
35PRA -
Garimella R, Kacena M, Tague S, Wang J, Horowitz M, Anderson HC. Expression of bone
low
morphogenetic proteins and their receptors in the bone marrow megakaryocytes of GATA-1
mice: A possible role in osteosclerosis. J Histochem Cytochem 2007; 55:745-52.
36PRA - Garimella R, Tague S, Zhang J, Belibi F, Nahar N, Sun B, Insogna K, Wang J, Anderson HC.
Expression and synthesis of bone morphogenetic proteins by osteoclasts: A possible path to
anabolic bone remodeling. J Histochem Cytochem 2008; 56:569-77.
37PRA -
Hofstaetter JG, Wang J, Yan J, Glimcher MJ. The effects of alendronate in the treatment of
experimental osteonecrosis of the hip in adult rabbits. Osteoarthritis Cartilage 2009; 17:362-70.
38PRA - Wang J, Gardner BM, Lu Q, Rodova M, Woodbury BG, Yost JG, Roby KF, Pinson DM, Tawfik O,
Anderson HC. Transcription factor NFAT1 deficiency causes osteoarthritis through dysfunction
of adult articular chondrocytes. J Pathol 2009; 219:163-72.
39PRA - Wang Q, Wang J, Lu Q, Detamore MS, Berkland C. Injectable PLGA based colloidal gels for
zero-order dexamethasone release in cranial defects. Biomaterials 2010; 31:4980-86.
40PRA - Schroeppel JP, Crist JD, Anderson HC, Wang J. Molecular regulation of articular chondrocyte
function and its significance in osteoarthritis. Histol Histopathol: Cell Mol Biol 2011; 26:377-94.
41PRA
Kramer WC , Hendricks KJ, Wang J. Pathogenetic mechanisms of posttraumatic
osteoarthritis: Opportunities for early intervention. Int J Clin Exp Med 2011; 4:285-98.
42PRA - Rodova M, Lu Q, Li Y, Woodbury BG, Crist JD, Gardner BM,Yost JG, Zhong XB, Anderson HC,
Wang J. Nfat1 regulates adult articular chondrocyte function through its age-dependent
expression mediated by epigenetic histone methylation. J Bone and Miner Res 2011; 26:197486.
43PRA-
Nahar NN, Tague SE, Wang J, Danley M, Garimella R, Anderson HC. Histological
characterization of bone marrow in ectopic bone, induced by devitalized Saos-2 human
osteosarcoma cells. Int J Clin Exp Med 2013; 6:119-25.
44PRA- Kruger TE, Miller AH, Wang J. Collagen scaffolds in bone sialoprotein-mediated bone regeneration.
Scientific World J 2013; Mar 31, E-Pub; doi:10.1155/2013/812718.
45PRA- Kruger TE, Miller AH, Godwin AK, Wang J. Bone sialoprotein and osteopontin in bone metastasis
of
osteotropic
cancers.
Crit
Rev
Oncol
Hematol
2013
Sep
7.
doi:
10.1016/j.critrevonc.2013.08.013. [Epub ahead of print]
Manuscripts in press: (MIP, provide names of all authors, title, journal, and PDF of manuscript plus
evidence of acceptance by journal.)
307
Manuscripts submitted but not yet accepted: (MS, provide names of all authors, title, journal,
and PDF of manuscript plus evidence of receipt of manuscript by journal.)
1MS-
Zhang M, Lu Q, Crist J, Theleman C, Zhong X, Brandt K, Wang J. Histone methylation-mediated
decrease in NFAT1 expression causes dysfunction of articular chondrocytes in aged mice.
Submitted to Arthritis & Rheumatism.
Invited or non-peer-reviewed articles or reviews: (Provide names of all authors, year, title,
journal, volume, and pages. If in press, provide documentation and PDF or article/review
if published within the last five years.)
Books and book chapters: (Provide names of all authors, year, book title, chapter title, edition,
publisher, and pages. If in press, provide documentation and PDF or book/book chapter if
published the last five years.)
1)
Wang J, Mah J, Glimcher MJ, Salih E. Biochemical changes during new bone formation in the
cranial and subcutaneous tissue environments. In: Davidovitch Z and Mah J editors. Biological
mechanisms of tooth eruption, resorption and replacement by implants. EBSCO Media,
Birmingham, Alabama, 1998. p. 123- 132.
2)
Wang J, Dong T. Pathophysiology and pathogenesis of osteonecrosis of the femoral head. In: Zu
CX, Chan ZG, Wong GT, and Liao YM editors. Diagnosis and treatment of avascular necrosis of
the femoral head: The advanced courses for clinical medicine. Chansao: Hunan Science and
Technology Publishing House; 1999. p. 81-88.
3)
Wang J, Dong T. Biomechanical changes during the repair process of avascular necrosis of the
femoral head. In: Zu CX, Chan ZG, Wong GT, and Liao YM editors. Diagnosis and treatment of
avascular necrosis of the femoral head: The advanced courses for clinical medicine. Chansao:
Hunan Science and Technology Publishing House; 1999. p. 88-93.
4)
Wang J, Dong T. Temporal changes in radionuclide bone imaging during the repair process of the
human femoral head necrosis after femoral neck fracture. In: Zu CX, Chan ZG, Wong GT, and Liao
YM editors. Diagnosis and treatment of avascular necrosis of the femoral head: The advanced
courses for clinical medicine. Chansao: Hunan Science and Technology Publishing House; 1999. p.
108-112.
5)
Zhou H-Y, Glimcher MJ, Wang J, Salih E. A novel glycosylated phosphoprotein, osteometrin. In:
Goldberg M, Boskey AL, Robinson C, editors. Proc of the Sixth International Conference on the
Chemistry and Biology of Mineralized Tissues. Rosemont, IL, Amer Acad Othop Surg, 2000.
p.185-188.
6)
Wang J, Glimcher MJ. Biomaterial-elicited bone repair and bone remodeling in cranial defects. In:
Davidovitch Z and Mah J, editors. Biological mechanisms of tooth eruption, resorption and
replacement by implants. EBSCO Media, Birmingham, 2000. p.9-16.
7)
Wang J, Zhou H-Y, Salih E, Gu X, Xu L, Hofstaetter JG, Glimcher MJ. Bone sialoprotein-collagen
complex elicits mineralization and ossification in a bone defect model. In: Landis W and Sodek J
(eds.), Proceeding of 8th International Conference on the Chemistry and Biology of Mineralized
Tissues. University of Toronto Press, Toronto, 2004; pp 139-142.
8)
Wang J, Dong T. Non-traumatic osteonecrosis of the femoral head. In: Dong T, Lu S, Jie S and Li
Z (eds), Surgery of Hip Joint. Zhengzhou University Press, Zhengzhou, 2005; pp391-403.
308
9)
Wang J, Kramer WC, Schroeppel JP. Transcriptional regulation of articular chondrocyte function and
its implication in osteoarthritis. In: Rothschild BM (ed), Principles of Osteoarthritis, ISBN 979-95351-0063-8. InTech, 2012; pp473-496.
Published abstracts: (Provide names of all authors (applicant’s name bolded), year, title, where
published, volume, and pages.) If the same work is reflected in a published abstract and a presentation
or poster, the work must only be listed only once.
01PA -
Wang J, Tong T, Tang T, Zu G. Results of vascularized (muscle) pedicle bone grafts for femoral
head necrosis after femoral neck fracture: Three to nine-year follow up in 87 cases.
Proceedings of the Seventh Annual Meeting of Orthopaedic Trauma Association, Seattle,
1991; pp115.
02PA -
Wang J, Maniwa S, Glimcher MJ. Temporal and spatial independence of bone and cartilage
induction by demineralized bone powder in cranial defects and subcutaneous tissue of rats. J
Bone Min Res 1994; 9(Supple 1):S181.
03PA -
Wu Y, Ackerman JL, Chesler DA, Neer R, Wang J, Glimcher MJ. Three dimensional bone
mineral density measurement by solid state phosphorus-31 magnetic resonance imaging. J
Bone Min Res 1995; 10(Supple 1):S477.
04PA -
Cao Y, Wang J, Perkins M, Vacanti CA. Injectable bone. Trans of 42nd Ann. Meet. Orthop
Res Soc 1996;21:619.
05PA -
Wang J, Glimcher MJ. Biochemical characterization of matrix-induced osteogenesis in rat
cranial bone defects. J Bone Min Res 1996; 11(Supple 1):S406.
06PA -
Salih E, Wang J, Glimcher MJ. Factor-independent protein kinase and its correlation with
calvarial defect repair induced by demineralized bone. J Bone Min Res 1996; 11(Supple
1):S407.
07PA -
Wang J, Mah J, Glimcher MJ, Salih E. Microsomal casein kinase II, bone
sialoprotein,
osteopontin and their correlation with ossification during calvarial bone repair. J Bone Min Res
1997; 12(Suppl 1):S426.
08PA -
Wang J, Yang R, Gerstenfeld L, Glimcher MJ. Molecular characteristics of bone differentiation
pathways during matrix-induced osteogenesis in rat cranial defects. J Bone Min Res 1997;
12(Suppl 1):S201.
09PA -
Wu Y, Chesler DA, Ackerman JL, Wang J, Glimcher MJ. Solid components of bone mtrix
measured by proton magnetic resonance imaging. J Bone Min Res 2001;16(Suppl 1):S463.
309
10PA - Wang J, Salih E, Zhou H-Y, Hofstaetter J, Glimcher MJ. Temporal and spatial characteristics of
th
bone sialoprotein-elicited in vivo biomineralization and ossification. Trans 49 Ann Meeting,
Orthop Res Soc 2003; 28:Paper #143.
11PA -
Wang J, Xu L, Wunderlich L, Glimcher LH, Glimcher MJ. Absence of nuclear factor of activated T
Cells (NFAT) transcription factor NFATp enhances endochondral ossification during healing of
th
long bone defects. Trans 50 Ann Meeting of Orthop Res Soc 2004; 29:Paper #66.
12PA -
Hofstaetter JG, Wang J, Yan J, Glimcher MJ. Changes in bone micro-architecture and mineral
density following experimental osteonecrosis of the femoral head in rabbits. Proceedings of the
th
5 Combined Meeting of the Orthopedic Research Societies of Canada, the U.S., Japan, and
Europe, Banff, Canada, 2004; 5:128.
13PA -
Wang J, Anderson AL, Lu Q. Cell-type-dependent effects of bone sialoprotein on
osteoblast differentiation. J Bone Miner Res 2006; 21(Suppl 1):S346.
14PA -
Yost JG, Lu Q, Rodova M, Wang J. Bone Sialoprotein-mediated osteoblast differentiation and
mineralization is principally dependent on the intrinsic characteristics of responding cells. J
Bone Miner Res 2007; 22(Suppl 1):S267.
15PA -
Rodova M, Gardner BM, Lu Q, Yost JG, Wang J. Runx2 and Canonical Wnt signaling
cooperatively regulate BMP-induced differentiation pathways of adult dural cells into osteoblasts
or chondrocytes. J Bone Miner Res 2008; 23 (Suppl 1):S384.
16PA -
Yost JG, Lu Q, Gardner BM, Rodova M, Wang J. Transcription factor NFATp regulates BMPinduced chondrocyte differentiation in a tissue-specific manner. J Bone Miner Res 2008; 23
(Suppl 1):S248.
17PA -
Wang J, Rodova M, Gardner BM, Lu Q, Trainor PA, Yost JD. Wnt/β-catenin signaling plays an essential
role in bone sialoprotein-elicited osteoblast differentiation and mineralization. Bone 2009;
44(Suppl 1):S158-9.
18PA -
Wang J, Gardner BM, Lu Q, Rodova M, Woodbury BG, Yost JG, Li Y, Zhong XB, Roby KF, Pinson
DM, Tawfik O, Anderson HC. Transcription factor NFAT1 deficiency causes osteoarthritis. J
Bone Miner Res 2009; 24(Suppl 1):S59.
19PA -
Rodova M, Lu Q, Li Y, Woodbury BG, Crist JD, Gardner BM, Yost JG, Zhong XB, Anderson HC,
Wang J. Dynamic Effects of Nfat1 and Sox9 on Articular Chondrocyte Function Associate with
Their Age- Dependent Expression and Epigenetic Histone Modifications. J Bone Miner Res
2010; 25(Suppl 1):S247.
20PA-
Wang J, Rodova M, Lu Q, Woodbury BG, Zhong XB, Anderson HC. Nfat1 regulates adult
articular chondrocyte function through its age-dependent expression mediated by
epigenetic histone methylation. Bone 2011; 48(Suppl 2):S142.
21PA-
Wang J, Lu Q, Rodova M, Crist JD. Bone sialoprotein stimulates intramembranous
ossification through Wnt/beta-catenin signaling pathway during bone repair. Bone 2011;
48(Suppl 2):S113.
310
22PA-
Lu Q, Rodova M, Bernard J, Wang J. Bone sialoprotein and Runx2 are interdependent
during osteogenic differentiation of adult mesenchymal stem cells. J Bone Miner Res
2011; 26(Suppl 1):S448.
23PA-
Wang J, Gardner B, Lu Q, Shaath M, Brandt K, Anderson H. Deficiency of NFAT1 transcription
factor causes osteoarthritis with alterations in articular cartilage and subchondral bone in adult
mice.
Osteoarthritis and Cartilage 2012; 20(Suppl 1):S60.
24PA
Lu Q, Furomoto B, Anderson HC, Wang J. Bone and muscle interations during the
progression of Nfat1 deficiency-mediated osteoarthritis. J Bone Miner Res 2012; 27(Suppl
1):S168.
25PA
Wang J, Lu Q, Shaath MK, Bernard J. Bone sialoprotein is essential for osteoblastic cell
differentiation and maturation. J Bone Miner Res 2012; 27(Suppl 1):S279.
26PA
Wang J, Lu Q, Yost J, Miller A, Garlich J. Bone sialoprotein stimulates the differentiation of
duraderived osteoprogenitor cells into osteoblasts during cranial bone repair. BoneKey
2013; 10 (Suppl1):S80.
27PA
Zhang M, Lu Q, Caldwell K, Crist J, Theleman C, Wang J. Decreased Nfat1 expression
contributes to dysfunction of articular chondrocytes in aging mice. J Bone Miner Res 2013;
28(Suppl 1):S195.
28PA
Zhang M, Lu Q, Bernard J, Miller A, Wang J. Epigenetic regulation of age-dependent Sox9
expression in mouse articular cartilage. J Bone Miner Res 2013; 28(Suppl 1):S362-363.
3
Presentations and posters in chronological order (oldest first, most recent last)
Oral paper presentations: (Provide names of all authors (applicant’s name bolded), title,
sponsoring organization, extent of peer-review, and location and date of presentation.)
1) Wang J, Glimcher MJ. Spatial independence of bone and cartilage induction by demineralized bone
matrix. International Conference on Bone Morphogenetic Proteins and Bone Reconstruction,
Bordeaux, France, June 15-16, 1995.
2) Wang J, Glimcher MJ. Biomaterial-elicited bone repair and bone remodeling in cranial defects. 3rd
International Conference on Biological Mechanisms of Tooth Movement and Craniofacial
Adaptation, Seoul, Korea, October 7-10, 1999.
3) Wang J, Salih E, Zhou H-Y, Hofstaetter J, Glimcher MJ. Temporal and spatial characteristics of
th
bone sialoprotein-elicited in vivo biomineralization and ossification. 49 Annual Meeting of
Orthopaedic Research Society, New Orleans, LA, USA, Feb. 2-5, 2003, Paper #143.
4) Wang J. Bone sialoprotein-elicited biomineralization and ossification. The 2nd Wittgenstein
311
Conference: Bone and Cartilage in Health and Disease, Vienna, Austria, October 17-19, 2003.
5) Wang J, Xu L, Wunderlich L, Glimcher LH, Glimcher MJ. Absence of nuclear factor of activated T Cells
(NFAT) transcription factor NFATp enhances endochondral ossification during healing of long bone
th
defects. 50 Annual Meeting of Orthopaedic Research Society, The Moscone Convention Center, San
Francisco, CA, USA, March 7-10, 2004.
6) Wang J, Zhou HY, Salih E, Gu X, Xu L, Hofstaetter JG, Glimcher MJ. Bone sialoprotein-collagen
th
complex elicits mineralization and ossification in a bone defect model. 8
Canada, Oct. 17- 22, 2004.
ICCBMT, Banff, Alberta,
7) Hofstaetter JG, Wang J, Samuel RE, Glimcher MJ. Alendronate in the treatment of experimental
osteonecrosis. 51th Annual Meeting of Orthopaedic Reseach Society, Washington DC, USA, Feb.
20-23, 2005.
8) Wang J, Rodova M, Lu Q, Gardner BM. Runx2 and canonical Wnt signaling in osteoblast or
chondrocyte differentiation. The 3rd International Congress of Chinese Orthopedic Association
Suzhou, China, Nov. 13- 16, 2008.
9) Wang J, Gardner BM, Lu Q, Rodova M, Woodbury BG, Yost JG, Roby KF, Pinson DM, Tawfik O,
Anderson HC. Transcription factor NFAT1 deficiency causes osteoarthritis through dysfunction of
st
adult articular chondrocytes. 31 Annual Meeting of the American Society for Bone and Mineral
Research, Denver, CO, USA, Mar 11-15, 2009.
10) Wang Q, Wang J, Lu Q, Detamore M, Berkland C. Injectable colloidal gels offer zero-order
dexamethasone release and cranial bone defect filling. The 37th Annual Meeting and Exposition of
the Controlled Release Society (CRS), Portland, Oregon, USA, July 10-14, 2010.
11) Wang J, Lu Q, Rodova M, Crist JD, Trainor PA. Bone sialoprotein stimulates intramembranous
ossification during cranial bone repair. 2011 Annual Meeting of the Orthopaedic Research Society,
Long Beach, CA, USA, January 13-16, 2011.
12) Wang J, Anderson HC, Lu Q, Zhang M, Furomoto B, Shaath MK. Deficiency of Nfat1 transcription
factor causes osteoarthritis through dysfunction of cells in multiple joint tissues. 2012 International
Conference on Orthopedics and Rheumatology, Chicago, IL, USA, August 13-15, 2012.
13) Zhang M, Lu Q, Caldwell KL, Crist JD, Theleman CL, Wang J. Histone Methylation-Mediated
Decrease in Nfat1 Expression Contributes to Dysfunction of Articular Chondrocytes in Aging Mice.
2013 Annual Meeting of the Orthopaedic Research Society, San Antonio, TX, USA, January 26-29,
2013.
14) Lu Q, Gardner B, Zhang M, Anderson HC, Wang J. Transcription factor Nfat1 regulates the expression of
nd
multiple catabolic and anabolic molecules in articular cartilage. 2 Joint Meeting of the International
Bone and Mineral Society and the Japanese Society for Bone and Mineral Research, Kobe, Japan,
May 28 - June 1, 2013.
15) Lu Q, Yost J, Miller A, Wang J. Bone sialoprotein-collagen implants promote cranial bone repair by
stimulating the differentiation of osteoprogenitior cells into mature osteoblasts. 8
of Orthopaedic Research Societies, Venice, Italy, October 13-16, 2013.
th
Combined Meeting
1) Wang J. Bone sialoprotein and bone regeneration. Orthopaedic Research Seminar Series for
312
Harvard Research Community, Boston, MA, USA, December 3, 2004.
2) Wang J, Gardner BM, Lu Q, Rodova M, Woodbury BG, Yost JG, Roby KF, Pinson DM, Tawfik O,
Anderson HC. Transcription factor NFAT1 deficiency causes osteoarthritis through dysfunction of
adult articular chondrocytes. Bone Day, University of Missouri at Kansas City School of Dentistry,
Sept. 4, 2009.
3) Shaath MK, Wang J. Nfat1 is a critical transcription factor for regulating the function of adult articular
rd
chondrocytes. 33
2011.
Annual Student Research Forum, University of Kansas Medical Center, March 31,
4) Kramer WC, Wang J. Transcription factor Nfat1 and posttraumatic osteoarthritis in mice. 2011 KU
Medical Center Resident Research Day, May 5, 2011.
5) Kramer, WC, Wang J. Nfat1 deficiency is a risk factor for the development of posttraumatic
osteoarthritis. 2011 Annual Meeting of Kansas Orthopedic Society, Kansas City, KS, Sept. 16, 2011.
6) Garlich J, Wang J. Role of Runx2 transcription factor in bone sialoprotein-mediated osteogenic
differentiation. 2012 Annual Student Research Forum, University of Kansas Medical Center, March
29, 2012.
7) Theleman C, Wang J. Nfat1 deficiency causes osteoarthritic subchondral bone changes through
pathological differentiation of bone marrow stem cells. 2012 Annual Student Research Forum,
University of Kansas Medical Center, March 29, 2012.
8) Caldwell K, Wang J. The role of Nfat1 in the healing of osteochondral defects. 2012 Annual Meeting
of Kansas State Orthopedic Society, Wichita, KS, Sept. 14, 2012.
9) Wang J. Epigenetic regulation of Nfat1 expression in articular chondrocytes and its implication in
osteoarthritis. Institute for Reproductive Health and Regenerative Medicine. Kansas City, KS, February
20, 2013.
10) Wang J. Molecular regulation of chondrocyte differentiation. Department of Anatomy and Cell
Biology Seminar, University of Kansas School of Medicine, Kansas City, KS, May 9, 2013.
Poster presentations in chronological order (oldest first, most recent last): (Provide names of all
authors (applicant’s name bolded), title, sponsoring organization, extent of peer-review and
location and date of presentation.)
Poster presentations at national and international meetings:
1) Wang J, Tong T, Tang T, Zu G. Results of vascularized (muscle) pedicle bone grafts for femoral head
necrosis after femoral neck fracture: Three to nine-year follow up in 87 cases. Seventh Annual Meeting
of Orthopaedic Trauma Association, Seattle, Washington, Oct. 31- Nov. 2, 1991.
2) Wang J, Maniwa S, Glimcher MJ. Temporal and spatial independence of bone and cartilage induction by
th
demineralized bone powder in cranial defects and subcutaneous tissue of rats. 16 Annual Meeting of
the American Society for Bone and Mineral Research, Kansas City, MO, Sept. 9-13, 1994.
3) Wu Y, Ackerman JL, Chesler DA, Neer R, Wang J, Glimcher MJ. Three dimensional bone mineral
th
density measurement by solid state phosphorus-31 magnetic resonance imaging. 17 Annual
Meeting of the American Society for Bone and Mineral Research, New York, Sept. 9-13, 1995.
313
4) Wang J, Glimcher MJ. Biochemical characterization of matrix-induced osteogenesis in rat cranial
th
bone defects. 18 Annual Meeting of the American Society for Bone and Mineral Research, Seattle,
Washington, Sept. 7-11, 1996.
5) Salih E, Wang J, Glimcher MJ. Factor-independent protein kinase and its correlation with calvarial
th
defect repair induced by demineralized bone. 18 Annual Meeting of the American Society for Bone
and Mineral Research, Seattle, Washington, Sept. 7-11, 1996.
6) Wang J, Mah J, Glimcher MJ, Salih E. Microsomal casein kinase II, bone sialoprotein, osteopontin and
th
their correlation with ossification during calvarial bone repair. 19 Annual Meeting of the American
th
Society for Bone and Mineral Research, Cincinnati, OH, 19 Annual Meeting of the American Society
for Bone and Mineral Research, Cincinnati, OH, USA, Sept. 10-14, 1997.
7) Wang J, Yang R, Gerstenfeld L, Glimcher MJ. Molecular characteristics of bone differentiation
th
pathways during matrix-induced osteogenesis in rat cranial defects (peer-reviewed). 19 Annual
Meeting of the American Society for Bone and Mineral Research, Cincinnati, OH, USA, Sept. 10-14,
1997.
8) Wu Y, Chesler DA, Ackerman JL, Wang J, Glimcher MJ. Solid components of bone mtrix measured by
proton magnetic resonance imaging. 23th Annual Meeting of the American Society for Bone and
Mineral Research, Phoenix, AZ, USA, Oct. 12-16, 2001.
9) Hofstaetter JG, Wang J, Yan J, Glimcher MJ. Changes in bone micro-architecture and mineral
th
density following experimental osteonecrosis of the femoral head in rabbits. 5 Combined
Meeting of the Orthopedic Research Societies of Canada, the U.S., Japan, and Europe, Banff,
Canada, Oct. 2-5, 2004.
10) Wang J, Anderson AL, Lu Q. Cell-type-dependent effects of bone sialoprotein on osteoblast
th
differentiation (peer-reviewed). 28 Annual Meeting of the American Society for Bone and
Mineral Research, Philadelphia, PA, USA, Sept. 15-19, 2006.
11) Yost JG, Lu Q, Rodova M, Wang J. Bone Sialoprotein-mediated osteoblast differentiation and
mineralization is principally dependent on the intrinsic characteristics of responding cells.
Honolulu, Hawaii, USA, Sept. 16-19, 2007.
12) Wang J, Rodova M, Gardner BM, Lu Q, Yost JG. Transcriptional regulation of articular chondrocyte
activity. Segal North American Osteoarthritis Workshop, Rush University, Chicago, IL, USA, May 2-4,
2008.
13) Rodova M, Gardner BM, Lu Q, Yost JG, Wang J. Runx2 and Canonical Wnt signaling cooperatively
regulate BMP-induced differentiation pathways of adult dural cells into osteoblasts or chondrocytes.
th
30 Annual Meeting of the American Society for Bone and Mineral Research, Montreal, Canada, Sept.
12-16, 2008.
14) Yost JG, Lu Q, Gardner BM, Rodova M, Wang J. transcription factor NFATp regulates BMP-induced
th
chondrocyte differentiation in a tissue-specific manner. 30 Annual Meeting of the American Society
for Bone and Mineral Research, Montreal, Canada, Sept. 12-16, 2008.
15) Wang J, Rodova M, Gardner BM, Lu Q, Trainor PA, Yost JD. Wnt/β-catenin signaling plays an essential
nd
role in bone sialoprotein-elicited osteoblast differentiation and mineralization. 2 Joint Meeting of the
International Bone and Mineral Society and The Australian & New Zealand Bone & Mineral Society,
Sydney, Australia, March 21-25, 2009.
314
16) Wang Q, Wang J, Detamore M, Berkland C. Injectable colloidal gels for drug delivery to bone defects.
2009 Annual Meeting of the American Association of Pharmaceutical Scientists (AAPS), Los Angeles,
California, November 11-14, 2009.
17) Woodbury BW, Rodova M, Lu Q, Gardner BM, Yost JG, Wang J. Runx2 and Wnt/β-catenin signaling
th
cooperatively suppress chondrogenic differentiation during BMP-induced cranial bone repair. 56
Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, March 6-9, 2010.
18) Rodova M, Lu Q, Li Y, Woodbury B, Crist J, Gardner B, Yost J, Zhong X, Anderson HC, Wang J.
Dynamic effects of Nfat1 and Sox9 on articular chondrocyte function associate with their ageth
dependent expression and epigenetic histone modifications. 32 Annual Meeting of the American
Society for Bone and Mineral Research, Toronto, Canada, Oct. 15-19, 2010.
19) Kramer WC, Lu Q, Wang J. Transcription factor Nfat1 deficiency: A risk factor for progression
th
of posttraumatic osteoarthritis in mice. 58
Society, San Francisco, CA, Feb. 4-7, 2012.
Annual Meeting of the Orthopedic Research
20) Lu Q, Furomoto B, Anderson HC, Wang J. Bone and muscle interactions during the progression of
Nfat1 deficiency-mediated osteoarthritis. American Society for Bone and Mineral Research Topical
Meeting: Bone and Skeletal Muscle Interactions, Kansas City, MO, July 17-18, 2012
21) Lu Q, Yost JG, Gardner BM, Wang J. Contribution of the dura to bone sialoprotein-mediated cranial
bone regeneration. 2012 International Conference on Orthopedics and Rheumatology, Chicago, IL,
USA, August 13-15, 2012.
22) Zhang M, Lu Q, Bernard J, Miller AH, Wang J. Epigenetic regulation of age-dependent Sox9
expression in mouse articular cartilage. 2013 Annual Meeting of the American Society for Bone and
Mineral Research, Baltimore, MD, USA, October 4-7, 2013.
23) Zhang M, Lu Q, Caldwell K, Crist J, Theleman C, Wang J. Decreased Nfat1 expression contributes
to dysfunction of articular chondrocytes in aging mice. 2013 Annual Meeting of the American
Society for Bone and Mineral Research, Baltimore, MD, USA, October 4-7, 2013.
Poster presentations at local and regional meetings:
1) Wang J, Anderson A, Lu Q. Cell-type-dependent effects of bone sialoprotein on osteoblast
differentiation. University of Kansas Medical Center Faculty Research Day, Kansas City, KS,
November 9, 2006.
2) Rodova M, Gardner BM, Lu Q, Yost JG, Wang J. Runx2 and Canonical Wnt signaling cooperatively
regulate BMP-induced differentiation pathways of adult dural cells into osteoblasts or chondrocytes.
University of Kansas Medical Center Faculty Research Day, Kansas City, KS, November 6, 2008.
3) Yost JG, Lu Q, Gardner BM, Rodova M, Wang J. Transcription factor NFATp regulates BMPinduced chondrocyte differentiation in a tissue-specific manner. University of Kansas Medical
Center Faculty Research Day, Kansas City, KS, November 6, 2008.
4) Wang J, Rodova M, Gardner BM, Lu Q, Trainor PA, Yost JG. Wnt/β-catenin signaling plays an essential role
in bone sialoprotein-elicited osteoblast differentiation and mineralization. University of Kansas Medical
Center Faculty Research Day, Kansas City, KS, November 3, 2009.
5) Wang J, Woodbury BW, Rodova M, Lu Q, Gardner BM, Yost JG. Runx2 and Wnt/β-catenin signaling
cooperatively suppress chondrogenic differentiation of adult mesenchymal stem cells. University of
315
Kansas Medical Center Faculty Research Day, Kansas City, KS, November 12, 2010.
6) Wang J, Lu Q, Yost JG, Miller AH, Garlich J. Bone Sialoprotein stimulates the differentiation of duraderived osteoprogenitor cells into osteoblasts during cranial bone repair. Bone and Muscle Day,
University of Missouri at Kansas City, School of Dentistry, Kansas City, MO, September 27, 2013.
7) Zhang M, Lu Q, Bernard J, Miller AH, Wang J. Epigenetic regulation of age-dependent Sox9 expression
in mouse articular cartilage. Bone and Muscle Day, University of Missouri at Kansas City, School of
Dentistry, Kansas City, MO, September 27, 2013.
8) Zhang M, Lu Q, Caldwell K, Crist J, Theleman C, Wang J. Decreased Nfat1 expression contributes to
dysfunction of articular chondrocytes in aging mice. Bone and Muscle Day, University of Missouri at
Kansas City, School of Dentistry, Kansas City, MO, September 27, 2013.
Invited seminars at other universities or institutions: (Provide title, sponsoring organization or institution,
and date of presentation.)
1) Cellular and molecular response to osteogenic morphogens implanted in bone defects.
Sponsored by School of Dentistry, Dankook University, Chunan City, South Korea, October 9,
1999.
2) Orthopedic tissue engineering. Sponsored by Soochow University Medical School, Suzhou, P. R. China,
June 20, 2002.
3) Cellular and molecular biology of bone repair. Sponsored by West Virginia University School of
Medicine, Morgantown, WV, May 13, 2004.
4) Genetic engineering in bone and cartilage regeneration. Sponsored by the Orthopedic Training
Program, the Ministry of Health, Suzhou, P.R. China, September 14, 2004.
5) In vivo function of bone sialoprotein in mineralization and osteogenesis. Sponsored by School of
Dentistry, University of Missouri at Kansas City, Kansas City, MO, June 21, 2006.
6) Advances in molecular orthopedics. Sponsored by the First Affiliated Hospital of Soochow
University, Suzhou, P. R. China, December 30, 2007.
7) Tissue-specific response to bone morphogenetic protein. Sponsored by Soochow University
Medical School, Suzhou, P. R. China, November 16, 2008.
8) Osteoarthritis: from mouse to man. Sponsored by the First Affiliated Hospital of Soochow
University, Suzhou, P. R. China, July 26, 2010.
9) Transcription factor NFAT1 Deficiency and osteoarthritis. Sponsored by Rush University Medical
Center, Chicago, IL, March 25, 2011.
10) The role of Wnt and BMP signaling pathways in bone sialoprotein-mediated osteogenesis.
Sponsored by Stowers Institute for Medical Research, Kansas City, MO, September 23, 2011.
11) Mechanisms of bone sialoprotein-specific osteogenic action. Sponsored by School of Dentistry,
University of Missouri at Kansas City, Kansas City, MO, November 30, 2011.
12) Grant applications for medical research: Opportunities and challenges. Sponsored by Soochow
University Medical School, Suzhou, P.R. China, March 9, 2012.
316
13) NFAT signaling in bone marrow stem Cells. Sponsored by Stowers Institute for Medical Research,
Kansas City, MO, May 7, 2012.
14) Posttraumatic osteoarthritis: A keynote lecture. Invited by the Organizing Committee of the
2012 International Conference on Orthopedics and Rheumatology, Chicago, IL, USA, August
13, 2012.
15) U.S Department of Defense (DoD) grant applications. Invited by the Orthopaedic Research Society
(ORS) and the Orthopaedic Research and Education Foundation (OREF) and presented at the 2013
ORS/OREF Grant Writing Workshop, San Antonio, TX, USA, January 25, 2013.
16) Posttraumatic osteoarthritis: Pathogenesis and novel therapeutic strategies. Invited by the University
of Southern California, Keck School of Medicine, Los Angeles, CA, USA, August 9, 2013.
4
Other evidence of scholarship
(includes clinical guidelines, policy documents, contributions to significant position statements by
professional organizations, and development of national examinations)
Patents:
Inventor
Title of Patent
Filing Institution
Jinxi Wang Prevention and Treatment
on 9/11/2012, of Osteoarthritis
8263546
KUMC
Date Filed
03/19/2009
Status
Issued by USPTO
US patent number:
Description of the Patent: Subjects lacking Nfat1 display osteoarthritis in weight-bearing joints. Osteoarthritic changes
associated with Nfat1 deficiency are characterized by articular cartilage degradation, articular chondrocyte proliferation
and clustering, progressive articular surface destruction, chondro-osteophyte formation, and exposure of thickened
subchondral bone. Methods for screening drug candidates that may be useful for prevention and treatment of Nfat1
deficiency-associated osteoarthritis are presented.
Inventor
Jinxi Wang
Title of Patent
Filing Institution
Prevention
KUMC
08/31/2012
Pending,
USPTO of Osteoarthritis: Divisional Application
317
Date Filed
and
Status
Treatment

Professional Resume - The University of Kansas

Transcription

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