QPix Systems - Labmate (Asia)

QPix Systems
An industry standard for microbial colony picking
www.moleculardevices.com/genetix
Genetix
Now part of Molecular Devices
Setting an industry standard
Although an essential step in many
different workflows, picking microbial
colonies can be a time-consuming, errorprone task.
Genetix systems replace tedious manual processes
with proven, objective and accurate solutions.
Imaging
Genetix colony pickers are
used worldwide with over 600
installations in research institutes,
sequencing facilities, biotech and
pharmaceutical companies.
Ensure high level performance at any scale
• High speed operation
• Objective image analysis
• Accurate colony picking
Analysis
Shorten timelines – increase productivity
• Plate and spread samples
• Accurately pick up to 30000 colonies/day
• Cherry-pick wells of interest after off-line analysis
• Replicate or re-array plates
Picking
Eliminate unnecessary work and expense
downstream
• Ensure clonality and high viability
• Eliminate risk of double picks, blank wells,
cross-contamination and manual picking errors
• Track plates, wells and clone location
02
Dedicated solutions tailored to workload
and applications
QPExpression: The greatest flexibility in multi-application laboratories
– fully automated from plating to picking.
• Plates and spreads samples into bioassay QTrays
• Images and picks up to 30000 microbial colonies
per day
• Cherry-picks selected clones after off-line analysis
QPix2 XT: Ideal for clone management, including library screening
• Images and picks up to 30000 microbial colonies
per day
• Cherry-picks selected clones after off-line analysis
• Sets up screening assays on filters or agar
03
The essentials of accurate colony picking
Since QPExpression offers the greatest flexibility in choice of application and
experimental design, the system is used here to illustrate the many details that
combine to ensure peak performance in colony picking.
Destination plate
when picking
Transformation/
sample source plate
for spreading
96 pipette tips for
plating onto QTrays
48-sectored bioassay QTrays
From plating to picking
Plates and spreads samples evenly
(QPExpression only)
Overnight incubation
04
Images
Normal view
Imaged view
nate
Elimsik of
ri over
carry
Pin sterilization process suitable
for any organisms -proven through
years of use in high throughput
sequencing centers.
Track identity of sample plates, wells
and picked clones via bar-code reader
Ensure adequate transfer of material
using organism-specific pin heads
Analyzes objectively – identifies and classifies colonies
based on size, shape, color and proximity to neighbors
Picks accurately - high precision
robotics pick only single colonies
User selection
Pick
Too small
Select predefined number
of colonies per
region for picking
Too close to
neighbor
05
Reliably supporting a wide range of applications
QPEx
press
ion
Protein engineering, protein evolution,
directed or enzyme evolution – shorten
timelines
Protein expression, transformation and
sub-cloning management – maintain
clonal integrity
• Objectively identify colonies according to pre-defined
• Automatically plate up to 96 transformations every
criteria, facilitating selection of the best colonies
• Control colony destination to optimize downstream
processing
• Accurately cherry-pick selected colonies and array
into wells for screening assays
30min.
• Automate and manage cloning and expression steps
-P
ick colonies into 96-well plates, duplicate as
required
- Accurately cherry-pick for further screening
QPExpression ensures controlled management
throughout screening and selection of large, diverse
populations.
• Improve handling efficiency e.g. only one manual
pipette step after ligation set-up
Successful screening and selection, requires
maintenance of clonal integrity and minimal errors when
handling serial reaction steps, multiple clones and Petri
dishes over a multi-day workflow.
ion
press
QPEx or
XT
QPix2
Phage display - increase productivity and
efficiency
• High capacity for larger screens
• High flexibility in experimental design
• Single colonies obtained by plating enriched library
selections
• Array on to filters for hybridization (QPix2 XT only)
QPix solutions are recommended by library providers
such as MorphoSys. They form an integral part
of antibody fragment discovery programs
of pharmaceutical suppliers.
06
ion
press
QPEx or
XT
QPix2
DNA sequencing –increase
productivity and reliability,
facilitate colony retrieval
• Eliminate risk of manual errors and DNA
carryover
• Track from sequence to well,
• Check ambiguous sequences
(match picker log data with sequencer
output)
• Track deep well plates used for colony outgrowth
prior to sequencing.
Used by major sequencing centers in the Human Genome
Project. Today QPix solutions isolate clones derived from poor
quality, degraded DNA, small nuclear-encoded regulatory RNA and
for disease mapping.
Clone Management –cost-effective DNA library
generation and management
QPix2
XT
• Cherry-pick selected clones with high accuracy
Unique gridding module of QPix2 XT
arrays picked colonies onto filters for
hybridization/screening. Extra stacker
capacity for destination
plates facilitates
high throughput
demands.
- Eliminate risk of double sequences or no growth
• Pick single colonies from sectored plates into deep well plates
for DNA isolation and mammalian cell transfection
• Create duplicate libraries and generate sub-libraries to maintain
clone viability and remove redundancy
• Replicate master libraries into working libraries
• Screen and identify 10 positive clones in a library of 100,000 clones
using sufficient probe to cover only 4 filters
• Array up to 25000 duplicates on a single filter for hybridization
QPix2 XT is the solution of choice when creating BAC and DNA libraries,
especially when providing an efficient alternative to time-consuming, errorprone library screening techniques.
07
Unrivalled solutions based on excellent imaging and intelligent image analysis
Products from Molecular Devices offer scientists
unrivalled solutions that utilize imaging and intelligent
image analysis to support basic research, pharmaceutical and biotherapeutic development. The company’s
systems continue to establish industry standards
in areas such as picking microbial colonies for genomic
studies or screening and selection of mammalian cell
lines. Other systems use imaging platforms to monitor
cell growth, evaluate cellular responses and quantify
protein production. Through its expertise in robotics, cell
and molecular biology, image analysis and interpretation,
supported by a strong IP portfolio, the company is
committed to the continual development of innovative
solutions for life science applications.
For more information, visit
www.moleculardevices.com/genetix
Imaging
Software
Dedicated software pre-installed on high specification PC, Microsoft®Windows® XP (Professional SP2)
White light imaging
Trans-illumination
Tracking
1 x barcode reader for tracking of source and destination plates
Instrumentation
Containment
Optional Class 100-type HEPA filtration
Source plate for sampling and spreading
1 x 96 well plate (QPExpression only)
Destination plate when spreading
2 x 22 cm QTrays (QPExpression only)
Picking destination plate type
Various, 96 or 384 well, including deep well
Picking source plate type/capacity
2 x 15 cm Petri Dish; 10 x 9 cm Petri Dishes; 4 x OmniTrays; 2 x 22 cm bioassay QTrays
Destination plate capacity
QPix2 XT: up to 210 low profile plates, 70 per stacker lane, maximum 3 stacker lanes
QPExpression: up to 140 low profile plates, 70 per stacker lane, maximum 2 stacker lanes
Picking head
Fully pneumatic, 96 pin picking head. Interchangeable heads for other applications
Picking pin size
Range of organism-specific pins
Picking capacity
30000 colonies per day
Wash bath
3 x Static wash baths
Picking system fluids
Bath 1: 1% bleach; Bath 2: dH20; Bath 3: ethanol
Pin drying
Proprietary halogen pin drying station
Gridding kit (optional)
QPix2 XT: capacity 6 x 22 cm membranes. QPExpression: not available
Plate replication (optional)
Software license and additional head
Re-arraying (optional)
Software license and additional head
Instrument dimensions
2850 mm (width incl. monitor arm x 730 (depth) x 2050 mm (height)
Instrument weight
411.5 kg (including table and compressor)
Compressor
Compressor unit
Clean, oil-free compressor with sub-micron filtration
Weight: Min. operating pressure:
Min. operating volume
60 kg: 6 bar: 80 L/min
Regulatory approval
CE marked
Also available:QPix2 - a basic, low throughput system
Source plate capacity
1 x 15 cm Petri Dish;
5 x 9 cm Petri Dishes;
2 x OmniTrays; 1 x 22 cm QTrays
Destination plate capacity
up to 15 plates
Tracking
optional hand-held; bar-code reader
Other optional functions
Gridding kit, plate replication, re-arraying
For a listing of trademark owners, visit www.moleculardevices.com/genetix
www.moleculardevices.com/genetix
08LBL1062.A1
ClonePix, CloneSelect, CellReporter are trademarks of Molecular
Devices (New Milton) Ltd. All third party trademarks are the property
of their respective owners.