QPix 400 systems

QPix 400 systems
Unmatched performance and productivity from
microbial colony pickers
with
Now ence
esc
Fluor ging
Ima
www.moleculardevices.com / genetix
Genetix
Now part of Molecular Devices
QPix 400 systems
- more than just colony pickers
The QPix 400 series of microbial colony pickers offer scientists far more than just accurate picking. As hundreds
to thousands of colonies are processed per day, significant advances in hardware and software ensure that the
right colonies are picked every time. Samples are tracked from spreading to picking, replicating and re-arraying
while the option to track fluorescent markers provides objective, quantitative information to facilitate colony selection.
Destination
plate when
picking
Stackers
for destination
plates
Bar-code reader
Agar height sensor
Picking tool
Transformation /
source plate
(1 x 96 wells)
Applicationdriven
software
96 pipette tips
for spreading
onto QTrays
48-sectored
bioassay
QTrays
QPix 460 offers greatest flexibility in application and experimental design
Now FLUORESCENCE offers unique information for colony selection when
combined with white light pre-screening
NEW!
Multiple fluorescent filters ensures compatibility with a wide range of fluorescent cloning vectors and enables
QPix 400 systems to reveal unique information about individual colonies when studying, for example, protein
folding, enzyme evolution and protein localization, to look for transformation markers or when screening
for mutations. Fluorescent imaging is available as an option on all systems.
Raw image shows differences in
expression (fluorescence) levels
02
Processed image of an entire plate in
a bioassay QTray highlights fluorescent
colonies
Meeting application and workload
requirements
The QPix 400 series have been developed from proven, world-class QPix robotics used in major sequencing
centers throughout the Human Genome Project. Routine applications such as phage display experiments
and synthetic biology are readily supported with a QPix 400 system.
From spreading to picking
Fully automate sampling and spreading (from a 96-well
source plate into bioassay QTrays) to grow colonies
prior to imaging and picking, makes the QPix 460 the
most flexible option in the QPix 400 range.
Choose a QPix 460 system for applications such as
protein engineering, protein evolution, directed
or enzyme evolution, protein expression,
transformation and sub-clone management.
QPix 460 prepares up to
48 samples per QTray
for overnight incubation
From imaging to picking
Capacity for a greater number of destination plates
makes the QPix 450 well-suited for applications
such as enzyme evolution, clone management,
library screening and biofuel development.
“QPix 450 outclasses rival
systems, handling fluorescent
picking with an unrivalled level
of ease and accuracy.”
Marc McCarthy, High Throughput Robotics Specialist,
Alimentary Pharmabiotic Centre, University College Cork, Ireland
03
Pick the right colony every time
– eliminate unnecessary work downstream
Unmatched performance ensures data quality, clonality and high viability. Advanced imaging and analysis,
together with precisely-controlled robotics, eliminate the risk of double picks or blank wells, manual picking errors
and cross-contamination, to ensure that the right colony is picked every time.
Image analysis software identifies individual colonies in white light
Colonies selected according
to user-defined parameters:
compactness, axis ratio, size
and proximity
White light
Yellow: Selected colonies
Red: Non-selected colonies
Optional pre-screening provides unique information to identify the best colonies
NEW!
White light
Colonies selected according
to user-defined parameters:
compactness, axis ratio, size,
proximity and fluorescent level
Fluorescence
Pre-defined number of colonies selected per region for picking
Yellow:
Colonies to be picked based on selection criteria
Green:
Desired number of colonies met
Red:
Desired number of colonies not met
04
Colonies picked with a typical efficiency >98%
a
A W
ith a sensor to detect agar
height, high precision robotics
pick single colonies gently and
accurately
b
B O
rganism-specific pin heads
ensure adequate transfer of
material
c
C A
proven pin sterilization
process is suitable for any
organism and specialist
sequencing
d
D A
bar-code reader tracks
sample plates, wells and
picked clones
Entire samples history tracked
Source Data
Colony Data
Destination data
Process and Receptacle Annotation
An entire history from sample spreading (QPix 460 only) to picking, replication and re-arraying is tracked. Important samples
can be tagged to enhance visibility of history and location and sample-specific data added.
05
Eliminate labor-intensive screening and
picking in many different applications
Fast track development of constructs or target proteins during protein expression
All colonies detected in white light
Colonies expressing required
level of target protein identified
in fluorescent channel
Selected colonies ready for picking
• Pre-screen to pick the right colonies
• Identify colonies that fluoresce at specific intensities of interest
• Automate and track entire workflow from spreading
of transformed cells (QPix 460 only) to colony picking
Confidently select candidate clones in synthetic biology
• Automatically image and select clones
of engineered micro-organisms
• Pick with high accuracy
• Track all experimental data
White light imaging of an entire plate
Efficiently screen phage display libraries to identify antibody candidates
• Automatically select and pick
Bind
Amplify
in E. coli
Wash
Elute
Phage display cycle
06
phage-containing E. coli colonies
Select colonies
for analysis
NEW!
Efficiently manage large, diverse populations
Use application-specific software modules to replicate, grid and re-array
Cherry-pick wells of interest into new plates
Replicate plates
1
2
3
4
Identical replicates (96 or 384 well)
Compression (4 x 96 into 1 x 384)
Expansion (1 x 384 into 4 x 96)
Imaging
Camera (white light
only system)
CCD Camera. Image resolution: 22 pixels / mm. Field of view: 62 x 46 mm
Camera (white light and
fluorescence system)
CCD Camera. Image resolution 22 pixels / mm. Field of view: 32 x 24 mm
White light imaging
Trans-illumination.
Fluorescent imaging
(optional)
Epi fluorescence illumination. Four standard wavelengths included:
Ex / Em: 377 / 447 nm for DAPI / Hoechst
Ex / Em: 457 / 536 nm for FITC / GFP / CFP
Ex / Em: 520 / 624 nm for Cy3 / Texas Red
Ex / Em: 628 / 692 nm for Cy5
Colony statistics
Colonies selectable based on size, proximity, roundness. Selection performed on whole tray image.
Tracking
1 x barcode reader for tracking of source and destination plates. Data tracked through all applications
for plates with same I.D.
Instrumentation
Spreading capability
(QPix 460 only)
Sampling & spreading source plate: 1 x 96-well. Aspiration volume 10 -130 µL.
Destination plate: 2 x 22 cm 48 region QTrays. Samples spread from pre-set or customizable pattern
Destination plate capacity
QPix 450: Up to 210 low profile plates, 70 per stacker lane, maximum 3 stackers
QPix 460: Up to 140 low profile plates, 70 per stacker lane, maximum 2 stackers
Source plate capacity
Without manual intervention: 2 x 15 cm Petri dish; 10 x 9 cm Petri Dishes; 4 x OmniTrays; 2 x 22 cm QTrays
Picking destination plate type
Various, 96 or 384 well, including deep well
Picking height
Integrated ultrasonic agar height sensor to set agar height per plate for accurate picking
Picking head
Fully pneumatic, 96 pin picking head. Interchangeable heads for other applications
Picking pin size
Range of organism-specific pins
Picking capacity
3000 colonies per hour in white light, 2000 colonies per hour in fluorescent light
Fluorescent picking
Colonies images in white light for location identification and fluorescence for data analysis.
WL and FL image multiplexed
Fluorescence data
Multiple parameters available e.g. interior mean. Fluorescent intensity recorded for picked colonies
Wash bath
3 x Static wash baths
Pin drying
Proprietary halogen pin drying station
Plate replication (optional)
Software license and additional head
Re-arraying (optional)
Software license and additional head
Dimensions
2200 mm (width, excluding monitor arm) x 800 mm (depth) x 2140 mm (height on table)
Contact your local Molecular Devices representative to discuss your exact requirements.
07
About Molecular Devices
Molecular Devices is one of the world’s leading
providers of high-performance bioanalytical
measurement systems, software and consumables
for life science research, pharmaceutical and
biotherapeutic development. Included within a
broad product portfolio are platforms for highthroughput screening, genomic and cellular analysis,
colony selection and microplate detection.These
leading-edge products enable scientists to improve
productivity and effectiveness, ultimately accelerating
research and the discovery of new therapeutics.
Molecular Devices is committed to the continual
development of innovative solutions for life science
applications. The Company is headquartered in Silicon
Valley with offices around the globe.
For more information about the complete product
portfolio, visit www.moleculardevices.com / genetix
ClonePix, CloneSelect, CellReporter are trademarks of Molecular
Devices (New Milton) Ltd. All third party trademarks are the property
of their respective owners.
www.moleculardevices.com / genetix
08LBL1062.A2
For a listing of trademark owners, visit www.moleculardevices.com / genetix