accessories handbook

Unlimited possibilities
Accessories Handbook
for NanoWizard, ForceRobot and CellHesion systems
®
®
®
valid from 2015
Unmatched flexibility & modularity
More than 100 accessories
More than 30 operating modes
the
NanoWizard , ForceRobot
®
®
and CellHesion family
®
CellHesion®® 200
Optional heads
NanoWizard®® ULTRA Speed A
for fast-scanning and super-resolution AFM
ForceRobot®® 300
NanoWizard
®
®
NanoScience and BioScience versions
Heads
Vortis™ A
Combi controller
Control
electronics
options
Software
Vortis A SPMControl
Can also drive the
JPK NanoTracker™
Experiment
Control™
Experiment
Planner™
System options
QI™
Advanced
3-axes
Manipulation
& lithography
Advanced
Force
Spectroscopy
Advanced
AC modes
CellHesion®
Module
TAO™
Module
BioMAT™
Motorized
precision stage
SPM Control &
DATA Processing
Direct
Overlay ™
PetriDishHeater™ for BioMAT™
Stages
Vortis™ A Advanced
SPMControl
™
™
TopView
Optics™
2-axes
Life sciences stage
HCS™
Standard stage
HybridStage™
Heating cooling stage
ECCell™ – Electrochemisty cell
Temperature
control
Fluid cells &
cantilever holders
Electrical modes
& application
modules
Extras & OEM
PetriDishHeater™
SmallCell™
ECCell™
BioCell™
Small volume
SmallCell™
Electrochemistry cell
Standard
Cantilever
Holder
HTHS™
HCM™
Heating cooling module
Supercut
Cantilever
Holder
Side-view
Cantilever
Holder
High temperature
heating stage
Cantilever
Holder with
electrical
tip connection
PetriDish
Holder
High Voltage
Sample Bias
Amplifier
Conductive
AFM
module
Kelvin
Probe
module
HyperDrive™
fluid imaging
package
CoverslipHolder
with electrical
sample
connection
Fast
scanning
package
8x
FluidicsModule™
Acoustic
enclosure
Vibration
isolation
Micromanipulator
Fiber coupled
detection module
Cameras
Setups and accessories for BioScience applications
highlighted with LIGHT BLUE BOXES
Setups and accessories for NanoScience applications
highlighted with GRAY BOXES
Typical BioScience applications and research fields:
Typical NanoScience applications and research fields:
High resolution imaging in air and liquid of single molecules such as proteins, DNA/
RNA, carbohydrates, lipids, Live Cell Imaging, bacteria and virus characterization,
cellular adhesion and cytomechanics, molecular interactions, receptor-ligand,
antibody-antigen, molecular unfolding, molecular recognition, biomaterials, compound and implant characterization, material properties such as adhesion, elasticity,
hardness; drug formulation, encapsulation and aging studies and many more
High resolution imaging in air and liquid of single molecules, thin films,
nanoparticles or nanowires, surface properties characterization such as adhesion,
stiffness, friction, conductivity, charges, hardness; polymer science, single
molecule force measurements, smart organic materials, surface properties of
conjugated polymers, biosensors, degradable materials, electrochemistry, colloidal
probe, surface forces and interface phenomena; and many more
For more detailed information about all options see the brochures and product notes or visit www.jpk.com.
2
CoverslipHolder
accessories handbook
Contents
1
2
3
4
Control electronics options
Vortis™ A SPMControl Station
Vortis™ A Advanced SPMControl Station
Vortis™ A Combi controller
4
7
System options
5
NanoWizard® 4a for high resolution and fast scanning
NanoWizard® ULTRA Speed A for fast-scanning and
super-resolution AFM
ForceRobot® 300
CellHesion® 200
TAO™ Tip Assisted Optics module
BioMaterials Workstation BioMAT™
CellHesion® module
Software modules
ExperimentControll™
ExperimentPlanner™ software module
QI™ Advanced software module
Advanced AC modes module
DirectOverlay™ module
NanoLithography/NanoManipulation module
MicroRheology module
Advanced Force Spectroscopy module
7
8
9
Applications modules and modes
8
Fast scanning package
HyperDrive™ fluid imaging package
OEM micropipettes
Electrical sample connection module
CoverslipHolder with electrical sample connection
Kelvin Probe (KPM) and Scanning Capacitance (SCM)
Microscopy module
Conductive AFM module
Conductive AFM module – Enclosed Volume
Tunneling Current Conductive AFM (TC-CAFM) module
High Voltage Sample Bias Amplifier
JPK 8x FluidicsModule™
ForceWheel™
5
Sample stages
HybridStage™
Motorized precision stage
Manual precision stage
Sample holder for large samples
Standard stage
11
6
Cantilever holder options
Fixed-spring cantilever holder
Standard cantilever holder
Supercut version cantilever holder
Cantilever holder with electrical tip connection
Side-view cantilever holder
12
Environmental control options –
fluid cells and temperature controls
BioCell™
CoverslipHolder
PetriDishHeater™
PetriDishHeater™ for BioMAT™
PetriDishHolder™
OEM Humidifier from LIS
SmallCell™ for harsh environments
3-port small volume SmallCell™
High Temperature Heating Stage (HTHS™)
Heating Cooling Module (HCM™)
Heating Cooling Stage (HCS™)
Cryostage
Electrochemistry solutions
Temperature controlled ECCell™
Heating Cooling Stage (HCS™) with
electrochemistry cell
OEM Potentiostats from IPS
Vibration and acoustic isolation
Active vibration isolation from Accurion
Active vibration isolation from Table Stable
Active air damping tables from TMC
JPK acoustic enclosure
JPK base for acoustic enclosure
14
17
18
10 Optics accessories
19
TopViewOptics™
TopViewOptics™ with boom stand
TopViewOptics™ for use with inverted optical microscopes
or the JPK BioMAT™
Raman reflector kit
Upright Fluorescence Microscope (UFM) Kit
Fiber coupled detection module
Inverted optical microscope configurations
11 OEM solutions and small parts
Stand for AFM head
JPK sample holder for small samples
JPK cable anchorage pillow
JPK cantilever changing tool
JPK biocompatible glue
JPK spare parts
OEM syringe pumps
CCD, EMCCD and sCMOS cameras
OEM indentation solutions
OEM materials testing modules for mechanical
properties
OEM incubators for Live Cell experiments under
controlled environments
OEM glove box systems for NanoWizard® AFMs
20
3
1 Control electronics options
Vortis™ A SPMControl Station
State-of-the-art digital controller with lowest noise levels.
Key features
High speed 16bit AD conversion with 60 MHz for
the photodetector signals
24 bit ultra precise ADC with 2.5 MHz
High speed lock-in amplifier technology for
precise amplitude and phase detection
High speed data capture with optional
burst mode
Modular hybrid analog/digital design with latest PPC technology (PowerPC @ 660 MHz)
Gigabit Ethernet interface for fast data link
Number of data points that can be captured
continuously: restricted only by HDD
Thermal noise acquisition up to 3.25 MHz
Vortis™ A Advanced SPMControl Station
The most advanced SPM controller. (see product note)
Key features
State-of-the-art digital controller with
lowest noise levels and highest number of
signal channels
4 high speed 16 bit ADC channels with 60 MHz
12 ADCs with 18 bit with 800 kHz
24 bit ultra precise ADC with 2.5 MHz
3 channels 14 bit DAC with 120 MHz
8 channels 24 bit DAC with 400 kHz
2 high-speed and 1 mid-speed lock-in amplifiers for precise amplitude and phase detection
High speed data capture
Modular hybrid analog/digital design with latest
PPC technology (PowerPC @ 660 MHz)
Gigabit Ethernet interface for fast data link
Number of data points that can be captured
continuously: restricted only by HDD
Thermal noise acquisition up to 3.25 MHz
2 gated photon counters
Vortis™ A Combi controller
The combined high-end solution for JPK Scanning Probe Microscopes
and Optical Tweezers systems. (see product note)
Key features
One system for all nano-force applications
Simple conversion from SPM to OT control
4 high speed 16 bit ADC channels with 60 MHz
12 ADCs with 18 bit and 800 kHz
3 ADCs with 18 bit and 100 kHz (for scanning)
24 bit ultra precise ADC with 2.5 MHz
3 high speed channels 14 bit DAC and 120 MHz
8 channels 24 bit DAC with 400 kHz
2 channels 16 bit DAC and 1.6 MHz
1 × 14 bit DDS with 240 MHz +/- 1 V @ 50 Ω
3 × 16 bit reference output up to 15 MHz
2 high speed lock-in amplifiers running with
60 MHz, frequency range from 1kHz to 10MHz
CellHesion®
4
1 mid speed lock-in amplifiers with 800 kHz, frequency range up to 300 kHz
Multiple feedback loops are implemented with fully
configurable transfer function
Phase-locked-loop (PLL) integrated for Frequency
Modulation
Thermal noise acquisition up to 3.25 MHz
Software controlled digital filters
High speed data capture
3 channels closed-loop control via capacitive
sensors
3 channels -20 to +120 V scanner drive
(4. channel optional)
NanoWizard®
ForceRobot®
NanoTracker™
2 System options
NanoWizard® 4a for high resolution and fast scanning
The new NanoWizard® 4a AFM head combines atomic resolution and fast-scanning
possibility with line rates up to 70Hz in a system with a large
scan range of 100 µm. (see NanoWizard® 4a brochure)
Key features
Tip-scanner technology for safe and easy operation
in air, gases, and fluids
Full transmission optical capabilities with standard
condenser; e.g. bright field, phase contrast,
Hoffman modulation or DIC simultaneous to AFM
imaging with inverted research microscopes
Advanced optical techniques simultaneous to AFM
like FRET, TIRF, FLIM, FCS, single molecule detection,
epi-fluorescence or confocal laser scanning (CLSM)
with optional optical microscopes
Simultaneous operation with optical super resolution techniques like STED, STORM/PALM
and SIM
JPK´s DirectOverlay™ feature for perfect
combination of AFM and optical microscopy
Open hard and software architecture
Optimum performance by active vibration
isolation table
Comprehensive set of AFM modes like JPKs
easy-to-use QI™ mode, contact mode with lateral
force, advanced AC modes like non-contact, phase
detection, electrical and magnetic imaging modes
NanoWizard® ULTRA Speed A
for fast-scanning and super-resolution AFM
The NanoWizard® ULTRA Speed A AFM head combines true atomic resolution and fast
scanning with line rates up to 300Hz. (see NanoWizard® ULTRA Speed A brochure)
Key features
Fast-scanning up to 300Hz line rate for tracking
dynamic processes
Comes with the easy-to-use QI™ mode for
quantitative material property mapping
Atomic resolution in closed-loop mode by lowest
scanner, position-sensor and detection-system
noise level
Unique integration with optical microscopy by
tip-scanning design and DirectOverlay™ feature
for most precise correlative microscopy
Fully flexible design with the widest range of
modes and accessories
ForceRobot® 300
The innovative force spectroscope with fully automated work flow. Take more than
80,000 force curves within 24 h while varying parameters such
as temperature or loading rate. (see ForceRobot® 300 brochure)
Key features
Fully automated force spectroscope with
highest flexibility
Cutting edge force spectroscopy and force mapping
in combination with single molecule fluorescence
Automated laser and detector alignment
CellHesion®
NanoWizard®
ForceRobot®
Automated cantilever drift compensation
Advanced and dedicated software with
ExperimentPlanner™, RampDesigner™ and
built-in data batch processing
Fits to the Vortis™ A controller
NanoTracker™
5
CellHesion® 200
The single cell force testing solution for cell adhesion and elasticity studies. Specialized
platform for cell adhesion and cell mechanics measurements from
single molecules to entire cells. (see CellHesion® 200 brochure)
Key features
Cantilever sensor lifting system with >110 µm travel
range with closed-loop control through high-speed
capacitive sensor feedback
Integrates with advanced optical imaging
(DIC, CLSM, TIRF, FRET...)
Fits to the Vortis™ A controller
TAO™ Tip Assisted Optics module
Specialized sample stage for advanced experiments combining AFM
and optical spectroscopy based on proven NanoWizard® technology.
2 axis and 3 axis version available. (see TAO™ module product note)
Key features
Ultimate platform for TERS, scatter type SNOM,
single molecule imaging/spectroscopy experiments
NanoOptical studies such as quenching, field
enhancement or bleaching
Integrated sample scanning confocal imaging
Integrates with conventional and advanced optical
imaging (DIC, Phase contrast, CLSM, TIRF, FRET...)
Flexible software system solution for experimental
freedom and remote operation
Ultimate flexibility through simultaneous control
of up to 6 scan axes with closed loop precision
(linearized with capacitive sensors):
AFM tip scanner: 100 × 100 × 15 µm3
Sample scanner:
100 × 100 × 10 µm3
100 × 100 µm2
30 × 30 × 10 µm3
Fits to the Vortis™ A Advanced controller
BioMaterials Workstation BioMAT™
Completely integrated system comprising upright optical microscope and AFM. The specialist
for investigations of opaque samples in life and material sciences. (see BioMAT™ brochure)
Key features
Studies on non-transparent substrates or samples
with AFM and optics
Outstanding reproducibility of the focussed
position (ROI) with both systems
Unique capability to investigate the ROI precisely
with optics and AFM
Also perfect during operation in liquids
Wide range of applications:
Biochips, cell chips or patterned substrates for cell
adhesion, plant cell applications, microbiology,
tissue engineering, ...
Flexible concept with shuttle stage
Both techniques can be operated even in different
laboratory rooms
CellHesion® module
As an add-on to the NanoWizard®, the CellHesion® module combines the capabilities
of the BioAFM with precise adhesion force measurements and all optical microscopy
features simultaneously. (see CellHesion® module product note)
Key features
Simultaneous focus tracking optional
Integrates with advanced optical imaging
100 µm additional z-travel
Perfect hardware linearization through
(DIC, Confocal microscopy, FRET...)
capacitive sensors
CellHesion®
6
NanoWizard®
ForceRobot®
NanoTracker™
3 Software modules
ExperimentControl™
Remote control and monitoring of complex and long-term experiments
Key features
Remote control and monitoring of complex and
long-term experiments via the internet by a PC,
tablet or smartphone
Simplifies setting up the instrument especially
inside an acoustic hood
Continuous status update of the instrument
Tablet with holder is included
ExperimentPlanner™ software module
For comprehensive planning of an experiment with all of the external parameters.
Key features
Scanners, motors, light sources, detectors,
environmental control options etc can be master
controlled and timed
Delivers full control over all system options
Design of advanced experiments
QI™ Advanced software module
For NanoWizard® systems; delivers quantitative mechanical properties. (see QI™ brochure)
Key features
Depending on application, it can also deliver
electrical conductivity or molecular recognition
in a single scan
Contact Point Imaging (CPI) mode for extremely
soft and inhomogeneous surfaces
QI™ Advanced imaging mode for conductive measu rements in combination with the JPK CAFM module
Any kind of sample can be imaged: samples with
steep edges, loosely attached samples, soft, sticky
and brittle samples
Works under ambient conditions and in fluid
Quick to learn and easy to operate
Provides additionally adhesion, stiffness and
dissipation data while scanning
Advanced AC modes module
Software module for NanoWizard® systems
Key features
Comprehensive range of advanced dynamic
feedback modes
Phase Modulation (PM), Frequency Modulation
(FM) or self-excitation FM
CellHesion®
NanoWizard®
ForceRobot®
Modes can be combined with
Amplitude Gain Control (AGC)
Higher harmonics with extra lock-in amplifier
Requires cantilever holder with DirectDrive™ capability
NanoTracker™
7
DirectOverlay™ module
Perfect integration of optical and AFM data – JPK‘s proprietary and
patented solution for perfect overlay of optical and AFM information.
Key features
Perfect overlay of optical and AFM data with
sub-diffraction limit precision
Direct “in optical image” selection of AFM
measurements (imaging and force curves)
Dramatic reduction of overview image scanning in
AFM, giving faster results & lower tip contamination
Optical image navigation to specific regions of
interest, even without AFM scanning. This protects
functionalized tips for molecular recognition,
avoiding tip passivation from image scanning before the force measurements.
NanoLithography/NanoManipulation module
Software module for NanoWizard® systems.
Key features
350nm
Free-hand or vector-based patterns are possible
Moving objects or scratching the substrate with
controlled force are possible
Pattern creation by electrical current such as
anodic oxidation
MicroRheology module
For visco-elastic properties of living cells and other samples such as gels or foams.
Key features
Extension to RampDesigner™ offering sine
modulation segments
Perform oscillating measurements with settings
for amplitude and frequency
Force history can be defined by the user through
choice of preceding and following segments in
RampDesigner™
Sine modulation analysis in the Data Processing software fits amplitude and phase of response
Advanced Force Spectroscopy module
For advanced force measurement experiments from single protein unfolding,
DNA stretching to probing of cells and tissue.
Key features
Tuneable frequency sweep from 0.1 to 10 kHz
Setting of user-defined patterns for rheology
applications
Integrated RampDesigner™ for user defined
segmentation of force curves
Advanced force clamp
4 Applications modules and modes
Fast scanning package
Fast scanning option for NanoWizard® 4a
Key features
> 70 Hz line rate for scan ranges up to 2 µm
Fast z-scanner and mechanical adaptation
Ultrastable JPK DirectDrive™ cantilever excitation
for AC modes
CellHesion®
8
>70 kHz z-scanner resonance frequency
Software upgrade for fast scanning
Supports FM-AFM and self-excitation FM-AFM
Fast data acquisition
NanoWizard®
ForceRobot®
NanoTracker™
HyperDrive™ fluid imaging package
For highest resolution imaging of small structured samples in liquid.
(see jpk website for more information)
Key features
Optional for Nanowizard® Nanoscience and Bioscience version
Minimized tip-sample interaction by using smallest amplitudes
Consists of a special cantilever holder with direct drive functionality, and a control software module
OEM micropipettes
JPK offers a micropipette system, e.g. CellTram® models from Eppendorf,
together with NanoWizard® or CellHesion® 200 systems.
Electrical sample connection module
Key features
Accommodates electrical conductive samples such as AFM metal stubs by magnetic fixation or transparent samples such as ITO coated glass coverslips
Insert for Life Science stages
CoverslipHolder with electrical sample connection
For electrical measurements such as Conductive AFM on a coverslip in
combination with high NA optics.
Key features
Accommodates electrical conductive samples and transparent samples such as ITO coated glass coverslips
Insert for Life Science stages
Kelvin Probe (KPM) and
Scanning Capacitance (SCM) Microscopy module
Option for nanoscale mapping of surface potential distribution.
Key features
External bias electronics module (+/-10 V)
Software interface
Enclosed volume version also available
Surface Potential Mapping
Electrical tip connection
CellHesion®
NanoWizard®
ForceRobot®
NanoTracker™
9
Conductive AFM module
For conductivity and I/V measurements on the nanoscale.
Key features
High-performance conductive AFM experiments
Recording I/V curves
Bias voltage range ± 10 V
Noise limit 0.55 pA RMS
Sample holders for opaque samples
Sample holder for transparent samples
works with all optical microscopes
Conductive AFM module – Enclosed Volume
For experiments in inert atmosphere; for conductivity and I/V measurements
on the nanoscale under controlled environmental conditions.
Key features
High-performance conductive AFM experiments
under user defined gas atmosphere
Recording I/V curves
Bias voltage range ± 10 V
Noise limit 0.55 pA RMS
Sample holders for opaque samples
Sample holder for transparent samples
works with all optical microscopes
Tunneling Current Conductive AFM (TC-CAFM) module
For low-conducting samples.
Key features
Highest-performance, lowest current conductive AFM experiments
Records local I/V curves
Bias voltage range ± 10 V
Current range with a gain 1*109 up to ± 10 nA
with a noise limit 100fA RMS in imaging bandwidth
Other current ranges on request
(see JPK Conductive AFM module product note)
Optional version with closed sample volume for
experiments in defined atmosphere with a volume
of 140 µL
In combination with QI™ Advanced mode and
NanoWizard® mechanical and electrical data can be correlated on the nanoscale in a single scan
With QI™ Advanced high resolution and low noise,
current mapping on fragile, brittle and loosely
attached samples with virtually no tip wear due to
eliminated lateral forces
High Voltage Sample Bias Amplifier
Can be used for biasing a sample, e.g., in electro-optical
experiments or in Piezo-Response Force Microscopy (PFM).
Key features
+/- 100 V can be applied between sample and tip
CellHesion®
10
Software controlled
NanoWizard®
ForceRobot®
NanoTracker™
JPK 8x FluidicsModule™
Key features
JPK FluidicsModule™ with up to 8 different liquids
such as buffer solutions
Software-controlled flow rate
Automated incubation or adding of chemicals
Comprehensive safety package
ForceWheel™
JPK‘s ForceWheel™ handheld accessory for most sensitive
experiment control, e.g., for force spectroscopy.
Key features
Continuous setting of gains and setpoints
Easy attachment of cells or beads with
cantilever tip
Connected via USB
Manual force curve acquisition
5 Sample stages
HybridStage™
Modular piezo based sample scanner stage combined with motorized coarse XY
sample movement. With the HybridStage™, accurate and highly reproducible Z-stacks,
important for image reconstruction, can be performed while the AFM stays in place.
Key features
Combined piezo and motorized stages for
automated mapping or scanning of large areas
Maps large objects such as cell scaffolds, printed
3D structures, micro-spheres or capsules and micro/
nano structured surfaces over millimeter distances
Cell/cell or cell/substrate adhesion in 3D with a
large pulling range up to 200 x 200 x 200 µm³
Mechanical properties of plant cells, cell layers, cartilage, parasites, scaffolds, bones or tissue typical
with rough surfaces over a mm range
Optional XY or XYZ piezo flexure scanners with a
large travel range (depending on the application)
can be implemented
Automated and streamlined workflow with the
motorized HybridStage™
Motorized precision stage
Offers automatic motion control for precise positioning
of the sample relative to optical axis and AFM probe.
Key features
Transmission illumination capability for
inverted optical microscopes like
Olympus IX line
Zeiss Axio Observer/Axiovert 100/135/200
Nikon TE 2000 or Ti series
Leica DMI/DMi lines
Please specify model upon order
Travel range of the sample 20 × 20 mm²
Step size (Resolution): ≤ 1 µm
Repeatability (unidirectional): ≤ 2 µm
CellHesion®
NanoWizard®
ForceRobot®
Maximum velocity: 1 mm/s
With joystick or software control
For automated tiling or mapping applications
Manual precision positioning of the AFM tip
Stand alone use or for inverted optical microscopes
Compatible with all JPK add-ons, glass slides,
Petri dishes etc.
Compatible with NanoWizard®, ForceRobot® 300
and CellHesion® 200 heads
NanoTracker™
11
Manual precision stage
Offers sub-micron resolution & fine motion control for precise
positioning of the AFM tip relative to optical axis and the sample.
Key features
Transmission illumination capability for
inverted optical microscopes like
Olympus IX line
Zeiss Axio Observer/Axiovert 100/135/200
Nikon TE 2000 or Ti series
Leica DMI/DMi lines
Please specify model upon order
Travel range of the sample 20 × 20 mm²
Precise positioning of the sample holder with
thermally decoupled, magnetic fixation
Flexible design for fluid cells, temperature control
options, and custom sample holders
Detachable from optical microscope for stand alone operation
Drift compensated design
Compatible with NanoWizard®, ForceRobot® 300
and CellHesion® 200 heads
Sample holder for large samples
The holder module fits directly to the manual or motorized precision stage.
Key features
The holder can accommodate larger samples such as microchips or wafers and is equipped with spring clips
which can be varied
Standard stage
Offers fine motion control for precise positioning of the AFM tip relative to the sample.
Key features
Flexible design for fluid cells and different sample
holders
Rigid, chemical resistive surface
Maximum flexibility for customized sample
mounting
Travel range of the AFM head
(tip positioning) 10 × 10 mm²
Stand alone use in combination
with JPK TopViewOptics™
Magnetic sample holder
6 Cantilever holder options
Fixed-spring cantilever holder
The standard holder for all-round applications.
Key features
For air or aqueous solutions
Compatible with JPK BioCell™, CoverslipHolder™
or PetriDishHeater ™ for easier navigation
Robust design using glass and medical steel
CellHesion®
12
Measurements in fluid droplets or in fluid baths like
Petri dishes or home made fluid cells
Immersible to 8 mm fluid depth
Easy to clean
Easy handling even with gloves
NanoWizard®
ForceRobot®
NanoTracker™
Standard cantilever holder
For all-round applications with a large optical field of view.
Key features
Chemically inert cantilever holder
Optimized for the use with top-view optics
Robust design using glass and medical
steel or gold spring only
Immersible to 6 mm fluid depth
Easy to clean by detergents, ultrasonic bath or autoclaving
Easy handling, even with gloves
Measurements in droplets or in fluid baths like
Petri dishes or home made fluid cells
Works in air and liquid, including aggressive media
Supercut version cantilever holder
The holder is designed for use in air or liquids with removable cantilever
spring for maximum cleaning results.
Key features
Chemically inert cantilever holder
For use with JPK BioCell™, CoverslipHolder™ or
PetriDishHeater ™ for easier navigation
Robust design using glass and medical steel or gold
spring only
Immersible to 6 mm fluid depth
Easy to clean by detergents, ultrasonic bath or
autoclaving
Easy handling, even with gloves
Measurements in droplets or in fluid baths like
Petri dishes or home made fluid cells
Works in air and liquid, including aggressive media
Cantilever holder with electrical tip connection
The holder is designed for use in air or liquids with a fixed
cantilever spring and an electrical tip connection.
Key features
For air or aqueous solutions
Compatible with JPK BioCell™, CoverslipHolder™ or
PetriDishHeater ™ for easier navigation
Measurements in fluid droplets or in fluid baths
like Petri dishes or home made fluid cells
Robust design using glass and medical steel
Immersible to 8 mm fluid depth
Easy to clean
Electrical tip connection
Side-view cantilever holder
This holder is designed for use in combination with inverted
optical microscopes to observe the cantilever region from the side.
Key features
For use with JPK BioCell™, CoverslipHolder™ or
PetriDishHeater ™ for easier navigation
Measurements in fluid droplets or in fluid baths like
Petri dishes or home made fluid cells
CellHesion®
NanoWizard®
ForceRobot®
Immersible to 8 mm fluid depth
Easy to clean
Easy handling, even with gloves
NanoTracker™
13
7 Environmental control options – fluid cells and temperature controls
BioCell™
The BioCell™ allows for the temperature-dependent study of living cells or single
molecules with the NanoWizard® AFM, CellHesion® 200 and ForceRobot® 300 in
their native environment. It is therefore particularly suited for applications in cell
biology and biomedical research. As an add-on for inverted optical microscopes the
NanoWizard® AFM in combination with the BioCell™ offers the unique possibility to
simultaneously study temperature-dependent processes like cell motility and metabolism with the AFM and simultaneous advanced optical imaging. During AFM measurement all the optical microscope‘s operation modes, in particular contrast techniques, remain constantly available. Even high-resolution fluorescence confocal
microscopy and single-molecule fluorescence techniques are possible since objective
lenses with highest numerical aperture can be used.
Key features
Connections for liquid perfusion and gas flow
(e.g., CO2 mix)
Fully compatible with JPK‘s range of NanoWizard®
AFM, CellHesion® and ForceRobot® systems
Drift-minimized in all direction
Insert for Life Science stage
Temperature range: 15 to 60°C
Resolution: 0.1°C
Quick-exchange cover slip bottom
CoverslipHolder
With the same design as the BioCell™ but without temperature control,
the CoverslipHolder is the ultimate high resolution liquid cell with coverslip bottom.
Key features
Insert for Life Science stage
Closed liquid cell with standard coverslip bottom
High-N.A. immersion optics possible by exchange able cover slip bottom (0.17 mm thickness)
Ports for perfusion & gas flow, e.g., CO2 or nitrogen
Superior design for combining AFM and confocal
laser scanning
Made of glass, steel or gold, and silicon only
Easy to clean, also in ultrasonic bath
Autoclavable inlet
PetriDishHeater™
The heater system is fully compatible with JPK‘s range of NanoWizard® AFM and CellHesion®
systems. This means all the standard imaging and measurement techniques may be applied
at temperatures up to 60 ˚C with 0.1 ˚C precision. These include fluorescence, brightfield,
phase contrast and DIC, AFM imaging and cell mechanics study. The system also offers perfusion control employing standard syringe pumps and gas flow of carbon dioxide possible
for long term cell studies. Glass bottomed dishes are important for high numerical aperture
fluorescence experiments such as confocal laser scanning used in combination with AFM.
Key features
Insert for Life Science stage
Temperature range: room temperature up to 60 °C
Compatible with 35 × 10 mm Petri dishes from WPI
and TPP - plastic or plastic with glass bottom
Adaptors for other suppliers such as Matek, Willco,
IWAKI, Ibidi, and BD upon request
CellHesion®
14
Ports for perfusion tubes and gas supply
(only available for certain types of Petri dishes)
Software control
Temperature stability 0.1 K
Fully compatible with JPK‘s range of NanoWizard®
AFM and CellHesion® systems
Fits to all inverted microscope brands in
combination with a JPK sample stage
NanoWizard®
ForceRobot®
NanoTracker™
PetriDishHeater™ for BioMAT™
Shuttle stage with integrated Petri dish heater for JPK BioMaterials workstation.
Key features
Temperature range: room temperature up to 60°C
Compatible with 35 × 10 mm Petri dishes from WPI
and TPP – plastic or plastic with glass bottom
Dipping lenses need to be compatible with type
of Petri dish
Temperature stability 0.1 K
Software control
PetriDishHolder™
Designed like the PetriDishHeater™, but without temperature control, the
PetriDishHolder™ is the ultimate liquid cell for high resolution experiments in
a Petri dish.
Key features
Insert for Life Science stage
Compatible with 35x10mm Petri dishes from WPI
and TPP (plastic or plastic with glass bottom)
Adaptors for other suppliers such as Matek, Willco, IWAKI, Ibidi, and BC upon request
Ports for perfusion tubes and gas supply (only available for certain types of Petri dishes)
Openings for immersion medium application
Silicone anti-evaporation seal
OEM Humidifier from LIS
Together with the BioCell™ and the PetriDishHeater™, JPK offers an external
humidifier from LIFE IMAGING SERVICE (LIS), consisting of:
Key features
Digital gas flow sensor (< 3 bar in; < 1 bar out)
Tubing (4 × 1 mm, 6 m)
Humidity sensor
Humidity sensor capsule
(from JPK, with 2 screws M 2.5×6 mm)
Humidity controller
Set of tubings
Insulating tubing (OD/ID - 4×2 mm)
Transfer tubing to fluid chamber
© LIFE IMAGING SERVICES
SmallCell™ for harsh environments
The perfect solution for smallest volume experiments in a hermetically sealed environment, the SmallCell™ is perfect for fluid or gas exchange. The SmallCell™ is compatible
with the JPK temperature control systems like the HTHS™, HCM™ and the HCS™.
Key features
Solvent, acid, chloroform and acetone resistant
Multi-purpose closed fluid cell for small
volumes (< 150 µl)
Inlet and outlet ports for perfusion experiments
CellHesion®
NanoWizard®
ForceRobot®
Glass, steel or gold, and resistive seal only
Easy to clean, also in ultrasonic bath
Autoclavable
Temperature range up to 100 °C
NanoTracker™
15
3-port small volume SmallCell™
The perfect solution for smallest volume experiments in a hermetically sealed environment,
the small volume SmallCell™ is perfect for aqueous solutions exchange. The SmallCell™ is
compatible with the JPK temperature control systems like the HTHS™, HCM™ and the HCS™.
Key features
Closed fluid cell for minimized volumes (< 60 µl)
Optimized for aqueous solutions
Inlet and outlet ports for perfusion experiments
with Luer connectors
Temperature range up to 80 °C
Extra port for small amounts of liquids such as drugs, proteins etc.
Polycarbonate, steel or gold, and seal only
Easy to clean, also in ultrasonic bath
Autoclavable
High Temperature Heating Stage (HTHS™)
This high performance heating stage is designed for demanding polymer applications
as well as for stable long time temperature studies of single molecules or nanoparticles.
Key features
Temperature range from ambient to 300 °C in air
and ambient to 80 °C in fluid when used together
with the JPK SmallCell™
Temperature resolution 0.1 °C
Drift minimized in all directions for full tempera ture ramps simultaneously with AFM imaging
No transmission illumination possible
Heating Cooling Module (HCM™)
Designed for heating and cooling experiments in gas or liquids with
minimized drift in all dimensions.
Key features
Temperature range from -30 °C to 120 °C
~0-80 °C temperature range for experiments
in aqueous solutions
Temperature resolution 0.1 °C
Compatible with JPK
Manual precision stage and
Motorized precision stage
No transmission illumination possible
Heating Cooling Stage (HCS™)
Designed for AFM experiments in air or liquids from 0 °C up to
100 °C with minimized drift in all dimensions – HCS™ follows the
same design criteria as HTHS™.
Key features
Temperature range from 0 °C to 100 °C
Temperature resolution 0.1 °C
Twin stage thermoelectric element
Drift minimized in all directions for full
temperature ramps simultaneous to AFM imaging
CellHesion®
16
Heatpipe design – no water cooling or fan required
Can be used together with closed fluid cells such
as SmallCell™
NanoWizard®
ForceRobot®
NanoTracker™
Cryostage
JPK offers specialized cryostages together with NanoWizard® systems
for cooling applications up to -120°C.
Please ask the JPK applications team for more information.
8 Electrochemistry solutions
Temperature controlled ECCell™
Electrochemistry with temperature control and transmission optics such as phase contrast, DIC or fluorescence together with AFM. The unique combination of temperature
controlled electrochemical measurements with high-end optics makes the ECCell™ the
ideal tool for combined studies of temperature and potential sensitive biomolecules.
Key features
Works with transparent or non-transparent
substrates
Accommodates standard coverslips,
metal or silicon chips or other substrates
Temperature range from ambient to 60 °C
max. 1350 µl liquid volume with perfusion capability
Sealed design for inert gas filling
Wire electrodes and miniature reference electrode
Suitable for conductive films or substrates
(e.g., ITO coated glass)
Compatible with common potentiostats
Allows the use of high numerical aperture lenses for optimum fluorescence performance
Unrestricted high resolution AFM imaging
Heating Cooling Stage (HCS™) with electrochemistry cell
Designed for electrochemistry AFM experiments with controlled heating
and cooling of the sample.
Key features
For opaque samples
Temperature range from 0°C to 100°C
Temperature resolution 0.1°C
Twin stage thermoelectric element
Drift minimized in all directions for full
temperature ramps simultaneous to AFM imaging
Heatpipe design – no water cooling or fan required
Top-view optical access for easy navigation
max. 1350 µl liquid volume with perfusion
capability
Sealed design for inert gas filling
Wire electrodes and miniature reference electrode
OEM Potentiostats from IPS
JPK offers third party potentiostats for electrochemistry applications together with
NanoWizard systems.
© IPS GmbH & Co. KG
CellHesion®
NanoWizard®
ForceRobot®
NanoTracker™
17
9 Vibration and acoustic isolation
Active vibration isolation from Accurion
Key features
Active Workstation 780 and 900 – active vibration
isolation workstations
i4 Series – compact table-top vibration
isolation unit
© Accurion GmbH
Active vibration isolation from Table Stable
Key features
TS 150 Small size table-top vibration isolation
system for loads up to 150kg
TS 140 medium size table-top system for loads
up to 140kg
© Table Stable Ltd.
Active air damping tables from TMC
Key features
For large setups such as AFM/Raman combinations
or confocal microscopy
For less-critical vibration level environments
© Technical Manufacturing Corporation
JPK acoustic enclosure
Approved for high performance applications: acoustic hood from
JPK for utmost stability and isolation.
Key features
Bio-acoustic enclosure for S1 level applications,
S2 lab compatibility possible on demand
Divisible in two parts for easy transport and setup
Special wall design made by sandwich construction
and acoustic foam for best damping results
2 front doors with windows
Dimensions: 1100 mm × 1100 mm × 1250 mm
Demands table with 1100 mm × 1100 mm area
JPK base for acoustic enclosure
Approved for high performance applications: base frame with top plate
from JPK for utmost stability.
Key features
Dimensions: footprint 1100 mm × 1100 mm
Height adjustable
Welded steel base frame
100 mm MDF sandwich table top
CellHesion®
18
NanoWizard®
ForceRobot®
NanoTracker™
10 Optics accessories
TopViewOptics™
The optical system for non transparent samples or substrates such as
HOPG, mica, ceramics, metall etc.
Key features
For opaque samples
Long working distance zoom lens from Navitar™
2 mm - 400 µm field of view
Köhler illumination, fiber light illumination
Color CCD camera
Easy positioning and focus adjustment
TopViewOptics™ with boom stand
The optical system for non transparent samples or substrates such as HOPG,
mica, ceramics, implants etc. Can be used in combination with inverted microscopes.
Key features
For opaque samples
Long working distance zoom lens from Navitar™
2 mm - 400 µm field of view
Köhler illumination, fiber light illumination
Color CCD camera
Easy positioning and focus adjustment
TopViewOptics™ for use with
inverted optical microscopes or the JPK BioMAT™
The optical system for non transparent samples or substrates such as HOPG,
mica, ceramics, implants etc. Can be used in combination with inverted microscopes.
Key features
For opaque samples
Long working distance zoom lens from Navitar™
2 mm - 400 μm field of view
Köhler illumination, fiber light illumination
Color CCD camera
Easy positioning and focus adjustment
Raman reflector kit
The optical system for Tip-Enhanced Raman Scattering (upright-TERS) on
opaque samples.
Key features
For opaque samples
Compatible with common inverted microscope
Raman spectrometer setups
CellHesion®
NanoWizard®
ForceRobot®
Fits to the JPK Raman reflector kit sample holder
and JPK TAO™ modules
NanoTracker™
19
Upright Fluorescence Microscope (UFM) Kit
Enables the combined use of AFM and upright fluorescence zoom microscopy for
co-localization experiments on opaque substrates.
Fiber coupled detection module
Allows the precise capture of light emitted by the SPM tip region into a fiber coupled
detector. Suitable for home-build SNOM or other light emitting tip experiments.
Key features
Fiber coupler for 50 µm core multi-mode optical
fiber (FC connector)
Fits to c-mount equipped inverted optical microscopes
Precise manual xy positioning of the fiber end
Built-in CCD-camera system for the alignment of
emission spot to fiber center
Three way optical switch for viewing sample, fiber
end, and semi-transparent overlay
Inverted optical microscope configurations
Research lines of inverted optical microscopes from Zeiss, Olympus, Nikon, and Leica.
Ask the JPK applications team for more information.
11 OEM solutions and small parts
Stand for AFM head
Parking stand for all NanoWizard®, CellHesion® or ForceRobot® 300 heads.
JPK sample holder for small samples
Adaptor for standard sample holder.
Holds magnetically fixed AFM metal stubs.
CellHesion®
20
NanoWizard®
ForceRobot®
NanoTracker™
JPK cable anchorage pillow
Heavy weight pillow for fixation of cables to reduce noise coupling.
JPK cantilever changing tool
Probe loading station for convenient cantilever exchange.
JPK biocompatible glue
For cantilever or sample fixation, easy removable and biocompatible.
JPK spare parts
Available on request.
Springs for different cantilever holders
Fluid cell seals
Adapters
Coverslips
Tubing for fluid cells
OEM syringe pumps
JPK offers syringe pumps from World Precision Instruments (WPI)
Aladdin AL-1000
Accepts syringe sizes: 1-60mL
Programmable, economical, versatile
Single pump
Up to 100 pumps can be daisy-chained together via
RS232 network
© World Precision Instruments Ltd.
Aladdin AL-4000
Accepts two different syringe sizes: smallest-60mL
Programmable, economical, versatile
Infuses and withdraws
Pumping rates: 1.459mL/hr-127.2mL/min
© World Precision Instruments Ltd.
CellHesion®
NanoWizard®
ForceRobot®
NanoTracker™
21
CCD, EMCCD and CMOS cameras
The DirectOverlay™ optical integration can be used with any camera.
The following CCD cameras are preferred and can be offered directly by JPK.
Andor® Technology
iXon+ EMCCD
Luca EMCCD
Clara Interline CCD
Zyla sCMOS
© Andor Technology plc.
Jenoptik ProgRes® cameras series, such as
MFcool
CFcool
© Jenoptik AG
The Imaging Source
FireWire CCD color camera
© Imaging Source Europe GmbH
IDS Imaging Development Systems
Compact USB CCD and CMOS cameras of type µEye®
© IDS Imaging Development Systems GmbH
CellHesion®
22
NanoWizard®
ForceRobot®
NanoTracker™
Photometrics
Evolve­™ EMCCD
© Photometrics
Hamamatsu Photonics K.K.
ORCA CMOS
© Hamamatsu Photonics K.K.
OEM indentation solutions
JPK offers different third party solutions for indentation.
OEM materials testing modules for mechanical properties
JPK offers different third party modules for in-situ AFM experiments under tensile, compression, bending or torsion load.
OEM incubators for Live Cell experiments under controlled environments
JPK offers different incubator models for LIVE CELL applications together with NanoWizard® or CellHesion® systems.
OEM glove box systems for NanoWizard® AFMs
The AFM system can be used in a glove box under controlled atmosphere.
© GS Glovebox Systemtechnik GmbH
© M. Braun Inertgas-Systeme GmbH
Please see dedicated brochures or product notes for more detailed information on our website at www.jpk.com.
CellHesion®
NanoWizard®
ForceRobot®
NanoTracker™
23
be more than a customer
– be our partner
Providing the best service and support worldwide is an essential part of JPK‘s philosophy. To accelerate this, JPK founded
global offices in Singapore, Tokyo, Shanghai, Cambridge (UK),
Paris (France) and Carpinteria (USA). JPK has the largest applications and service team specialized in life science and nano
science applications to support your research.
Our representatives in different countries are hand-selected
to offer a service which meets the JPK quality standards.
We achieve this through long-term partnership and by
continuous training of applications scientists and service
engineers. For more detailed questions our JPK applications
team is just an email or phone call away from you.
JPK WORLDWIDE
JPK offers regular user trainings and courses for beginners‚
or experts in our imaging facilities worldwide. Here users
can learn to operate new instruments or get tips and tricks
ranging from experimental procedures to sample preparation.
BERLIN
HEADQUARTERS
UK
FRANCE
USA
SHANGHAI
JAPAN
SINGAPORE
To find a representative in your region visit our website or call us.
The handbook reflects a large variety of options and will grow further with latest developments. If you cannot find an accessory or
operating mode in this handbook ask us directly. JPK is able to deliver customized solutions even for advanced applications.
NanoWizard, CellHesion, TAO, BioMAT, NanoTracker, ForceRobot, Vortis, DirectOverlay, QI, HyperDrive, RampDesigner, ExperimentPlanner, ExperimentControl, ForceWatch, ForceWheel, FluidicsModule, HybridStage,
BioCell, SmallCell, ECCell, HTHS, HCS, HCM, TopViewOptics, PetriDishHeater are trademarks or registered trademarks of JPK Instruments USA, Inc. All information and specifications are subject to change without notice.
All rights reserved. © 2015, JPK Instruments USA, Inc.
JPK Instruments USA, Inc.
4189 Carpinteria Avenue
Suite 1
Carpinteria, CA 93013
tel.: +1 408 807 8878
usa.jpk.com
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be used for an offer in:
USA China Japan
Europe & other regions
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