TT2005 - International Society for Transgenic Technologies

TT2005
6th Transgenic Technology Meeting
11-13 September 2005, Barcelona, Spain
Auditorium of Sant Joan de Déu / Hotel Abba Garden
TT2005
6th Transgenic Technology Meeting
TT2005 - Barcelona
Barcelona, 11 September 2005
Dear participant,
Welcome to Barcelona! On behalf of the TT2005 Organising Committee, I thank you for coming
to Barcelona and I wish you a pleasant and fruitful stay in Barcelona during these days. This will
be the sixth Transgenic Technology (TT) meeting organised to date, and the first one going to
Southern Europe. In the next pages you can find some information regarding the past and
current TT meetings.
We have carefully prepared a scientific and technical program that we hope will be enjoyable for
all of you, scientists and technicians. We have invited a mixture of leading scientists and
experienced professionals within the animal transgenesis field, aiming to discuss the latest
advances and developments and the current state-of-the-art in the area.
The TT2005 meeting in Barcelona has been possible thanks to the commitment of the two
organising institutions: the Barcelona Science Park (Parc Científic de Barcelona, PCB, Barcelona)
and the National Centre of Biotechnology (Centro Nacional de Biotecnología, CNB, Madrid). In
addition, we have obtained the support of 8 public institutions and 23 private companies, all of
which have kindly agreed to sponsor the TT2005 meeting.
In this participant’s meeting booklet, you will find the latest information, the final TT2005
program, the full list of participants and the collection of additional abstracts that will be
presented at this meeting, which could not be included in the distributed August issue of the
scientific journal Transgenic Research.
Lastly, I would like to recommend you to spare some time, before or after the meeting, to visit
the beautiful city of Barcelona. Please, spend some time looking around and enjoying some of
the wide tourist offer, including: the “Eixample”, the original square-shape distribution of
buildings genuinely devised by I. Cerdà in the XIX century, the modernist architecture buildings
and parks designed by Antoni Gaudi, the old (gothic) district, the beach(es) and all the
promenades by the Mediterranean sea, museums (Picasso, Miro, Modern Art, History, …), the
Olympic areas from Barcelona-92, the fountains of Montjuïc….
We thank you once again for coming to Barcelona and for your interest in the TT2005 meeting.
Warmest regards,
Lluís Montoliu
TT2005 Organising Committee, Chair
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TT2005
6th Transgenic Technology Meeting (TT2005)
11-13 September 2005, Barcelona, Spain
Auditorium of Sant Joan de Déu / Hotel Abba Garden
(Hospital Sant Joan de Déu, HSJD, Barcelona)
TT2005 Organising Committee:
Lluís Montoliu (CNB-CSIC, Madrid)
Johannes Wilbertz (Karolinska Institutet, KCTT, Stockholm)
Nelson Khoo (Umeå University)
Sagrario Ortega (CNIO, Madrid)
Belén Pintado (INIA, Madrid)
Anna Pujol (CBATEG, UAB, Barcelona)
Mariona Arbonés (CRG, Barcelona)
MªCarmen Muñoz (PCB, Barcelona)
TT2005 Meeting Secretariat:
Stella Grimpa (PCB, Barcelona)
Susana Ayerdi (CNB-CSIC, Madrid)
TT2005 Organising Institutions:
Parc Científic de Barcelona (Barcelona Science Park, PCB) [www.pcb.es]
Centro Nacional de Biotecnología (CNB-CSIC, Madrid)
[www.cnb.uam.es]
www.cnb.uam.es/~tt2005/
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TT2005 Sponsoring Institutions:
Ministerio de Educación y Ciencia (MEC)
[www.mec.es]
Consejo Superior de Investigaciones Científicas (CSIC)
[www.csic.es]
Fundación Genoma España
[www.gen-es.org]
Universitat Autònoma de Barcelona (UAB)
[www.uab.es]
Centro Nacional de Investigaciones Oncológicas (CNIO, Madrid)
[www.cnio.es]
Centre de Regulació Genòmica (CRG, Barcelona)
[www.crg.es]
Sociedad Española de Bioquímica y Biología Molecular (SEBBM) [sebbm.bq.ub.es]
Sociedad Española para las Ciencias del Animal de Laboratorio (SECAL)
[www.secal.es]
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TT2005 Sponsoring Companies:
Springer / Transgenic Research
[www.springeronline.com]
Charles River Laboratories España S.A.
[www.criver.com]
Ozgene Pty Ltd.
[www.ozgene.com]
Harlan Interfauna Iberica S.L.
[www.harlan.com]
Leica Microsystems AG
[www.leica-microsistemas.com]
genOway
[www.genoway.com]
B&K Universal Ltd.
[www.bku.com]
Eppendorf AG
[www.eppendorf.com]
Nucleis SA
[www.nucleis.com]
Sigma-Aldrich Química,S.A.
[www.sigma-aldrich.com]
Dietex International Limited t/a Special Diets Services
[[email protected]]
BioMedical Instruments
[[email protected]]
Hamilton Thorne Biosciences, Inc.
[www.hamiltonthorne.com]
Ingenium Pharmaceuticals AG
[www.ingenium-ag.com]
Scanbur BK A/S
[www.scanbur-bk.com]
Xenogen Biosciences
[www.xenogen.com]
Polygene Inc.
[www.polygene.ch]
The Glassworx Company
[www.theglassworx.com]
Roche Diagnostics, S.L.
[www.roche-applied-science.com]
Artemis Pharmaceuticals GmbH
[www.artemispharma.de]
Olympus Optical España
[www.olympus-europa.com]
Hamamatsu Photonics
[www.hamamatsu.com]
BLS-Biomedical Laboratory Equipment, Maintenance and Service Ltd.
[www.bls-ltd.com]
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TT2005 Meeting Program
Sunday, 11st September 2005
08:00 –13:00
Setting up stands at the Auditorium of Sant Joan de Déu
Setting up stands at the Hotel Abba Garden
13:00 – 15:00
Registration at the Auditorium of Sant Joan de Déu
Setting up posters and stands
Poster will be shown at the Hotel Abba Garden
15:00 - 15:10
Welcome address. Director of PCB, TT2005 Coordinators
15:10 - 15:20
Presentation of the meeting. TT2005 Coordinators
Session I: Vertebrate Non-mammalian transgenic animals
Co-chairs: Lluis Montoliu (CNB, Madrid) – Nelson Khoo (Umeå Univ., Sweden)
15:20 – 15:50
Transgenic Fish: Complementary models to mammalian systems for
studying gene function
Robert N. KELSH
Centre for Regenerative Medicine, Univeristy of Bath, UK
15: 50 – 16:20
Mammalian germ-line transgenesis by transposition, sleeping beauty
transposon system. from fish to mice
Junji TAKEDA
Osaka University, School of Medicine, Osaka, Japan
Session II: genOway Prize for Transgene Technologies
16:20 – 16:30
Presentation of genOway Prize for Transgene Technologies
Kader THIAM
genOway, Lyon, France
16:30 – 17:15
Talk given by the TT2005 genOway Prize Winner
Andras NAGY
Mount Sinai Hospital, Samuel Lunenfeld Research Instiute, Toronto,
Canada
17:15 – 17:45
Coffee break, Visiting Posters and Stands
Hotel Abba Garden
Session III: New technologies in Transgenesis
Co-chairs: Lluis Montoliu (CNB, Madrid) - Nelson Khoo (Umea Univ., Sweden)
17:45 – 18:15
The ET-cloning system and beyond
A. Francis STEWART
Technische Universität Dresden, BioInnovationZentrum, Dresden,
Germany
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18:15 – 18:45
Use of lentiviral-type vectors in transgenic animals
Bruce WHITELAW
Roslin Institute (Edinburgh), Roslin, UK
18:45 – 19:15
RNAi in expression vectors
Michaela SCHERR
Medizinische Hochschule, Hannover, Germany
19:15 - 19:45
Monitoring CRE recombination at cellular level, FLEX transgenesis
Norbert B. GHYSELINCK
Institut de Génétique et de Biologie Molléculaire et Cellulaire
(IGBMC), Illkirch (Strasbourg), France
20:00 – 21:30
Get-together Buffet offered to participants
Visiting Posters and Stands
Hotel Abba Garden
21:30
Barcelona sightseeing at night:
The fountains of Montjuïc, The Magic Fountain
Bus departure at 21:30 in front of Hotel ABBA GARDEN
Monday 12nd September 2005
Session IV: Generation and Breeding of Transgenic and Knockout Mice
Chair: Johannes Wilbertz (Karolinska I., Sweden)
9:00-9:30
Choice of Strains/Strain Genetic-Differences/Modifiers
David WOLFER
Institut of Anatomy, University of Zürich, Zürich, Switzerland
9:30-10:00
Welfare and characterization of genetically modified mice
Rikke THON
Harlan, Denmark
10:00-10:30
Mouse Colony Management (Databases)
Marie-Noelle MARTINEZ
Charles River Laboratories, France
10:30-11:00
Refinement techniques in Transgenesis
Dominic J. WELLS
Department of Cellular and Molecular Neuroscience, Imperial College
Faculty of Medicine, London, UK
11:00 – 11:30
Coffee-Break
Visiting Posters and Stands
Hotel Abba Garden
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Session V: Funtional genomics. Using mice/transgenic mice to understand
Complex Mammalian Genomes
Chair: Mariona Arbones (CRG, Barcelona)
11:30-12:00
The Eumorphia Project
Steve BROWN
MRC Mammalian Research Unit, Harwell, UK
12:00-12:30
European Mouse Mutant Archive (EMMA)
Martin HRABÉ DE ANGELIS
GSF National Research Center for Environment and Health, Institute
of Experimental Genetics, Neuherberg, Germany
12:30-13:00
Public Gene Trap Initiative
Harald VON MELCHNER
Laboratory for Molecular Hematology, University of Frankfurt Medical
School, Frankfurt am Main, Germany
13:30-15:30
Lunch
Visiting Posters and Stands
Hotel Abba Garden
Session VI: Running a Transgenic Unit. Round-table discussion
Co-chairs: Johannes Wilbertz (Karolinska I., Sweden) / Belen Pintado (INIA, Madrid,
Spain)
15:30-17:30
Frank ZIMMERMANN
Biotechnologie Labor (Transgenic Unit), University of Heidelberg,
Heidelberg, Germany
Francina LANGA
Centre d'Ingénierie Génétique Murine (CIGM), Institut Pasteur, Paris,
France
Sagrario ORTEGA
Transgenic Unit, Biotechnology Program, Centro Nacional de
Investigaciones Oncológicas (CNIO), Madrid, Spain
Thom SAUNDERS
Transgenic Animal Model Core, University of Michigan, Ann Arbor,
Michigan, USA
Shirley PEASE
Transgenic and Knockout Core Facility, California Institute of
Technology (Caltech), Pasadena, California, USA
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Session VII: Non-invasive methods: Imaging
Chair: Sagrario Ortega (CNIO, Madrid)
17:30-17:50
3D imaging
James SHARPE
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK
17:50-18:10
In vivo imaging
Marc JANIER
ANIMAGE – CREATIS / Hôpital Edouard Herriot, Lyon, France
18:10-18-30
Molecular Imaging: Structural and functional techniques
Manuel DESCO
Laboratorio de Imagen. Medicina Experimental. Hospital General
Universitario "Gregorio Marañón", Madrid, Spain
18:30-18:50
Use of tracers (luciferase) in vivo
Paul KRIMPENFORT
Division Molecular Genetics, The Netherlands Cancer Insitute,
Amsterdam, The Netherlands
Session VIII: Founding an European/International Transgenic Tech. Society?
19:00-20:00
Round-table/Debate:
Lluis Montoliu / Johannes Wilbertz / Nelson Khoo
21:30
TT2005 Dinner and Live Music
Restaurant “La Miranda” (Museum History of Catalonia, 4th floor)
Complimentary Bus departure at 21:00 in front of Hotel
ABBA GARDEN (please book in advance)
Tuesday 13rd September 2005
Session IX: Phenotypic Analysis of Genetically Modified Mice
Co-chairs: Anna Pujol (CBATEG, UAB, Barcelona), Belen Pintado (INIA, Madrid)
9:00-9:20
Clinical physiology of genetically modified mice
Raymond ROMAND
Institut de Génétique et de Biologie Molléculaire et Cellulaire
(IGBMC), Illkirch (Strasbourg), France
9:25-9:45
Pathology of genetically modified mice
Pierre DUBUS
Department of Histology and Molecular Pathology. University and
Hospital of Bordeaux. Bordeaux, France
9:50-10:10
SHIRPA/Behavioral analysis of genetically modified mice
Mara DIERSSEN
Genes and Disease Program, Genomic Regulation Center, Barcelona,
Spain
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10:15-10:35
Breeding schemes: Speed congenics / Standard congenics
Jean-Louis GUÉNET
Institut Pasteur, Paris, France
10:40-11:10
Phenotypic analysis of Metabolic Diseases: diabetes
Fàtima BOSCH
CBATEG, Barcelona, Spain
11:15-11:45
Coffee Break
Visiting Posters and Stands
Hotel Abba Garden
Session X: Hot topics in mouse genetics and development
Co-Chairs: Mariona Arbones (CRG, Barcelona), Lluis Montoliu (CNB, Madrid)
11:45-12:15
Genomic Imprinting
Gavin KELSEY
The Babraham Institute, Cambridge, UK
12:15-12:45
Cell cycle and mouse genetics
Marcos MALUMBRES
Molecular Oncology Program, Centro Nacional de Investigaciones
Oncológicas (CNIO), Madrid, Spain
12:45-13:15
Mouse genetics and CNS disorders
Xavier ESTIVILL
Genes and Disease Program, Genomic Regulation Center, Barcelona,
Spain
13:15-13:30
A new method to produce mice fully derived from embyonic stem
cells
David VALENZUELA
Regeneron Pharmaceuticals, Tarrytown, NY, USA
13:35-13:40
Concluding Remarks.
Lluís Montoliu
13:40-13:45
Next TT meeting in Brisbane (Australia)
Elizabeth Williams
Transgenic Animal Service of Queensland. Institute for Molecular
Biosciences. University of Queensland, Australia.
14:00-16:00
Lunch.
Visiting Posters and Stands
Hotel Abba Garden
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Course on Basic Transgenic Techniques
16:00-16:30
Construct Design for transgenes and gene targeting experiments
Alfonso GUTIÉRREZ-ADÁN
Departamento de Reproducción Animal, INIA, Madrid, Spain
16:30-17:00
ES cell culturing (cells, methods, problems)
Sagrario ORTEGA
Transgenic Unit, Biotechnology Program, Centro Nacional de
Investigaciones Oncológicas (CNIO), Madrid, Spain
17:00-17:30
What strains are the best for transgenic/knock-out experiments?
Tom FIELDER
Transgenic Mouse Facility, UC-Irvine, Irvine, California, USA
17:30-18:00
Microbial agent impact on animal experiments
Jose Manuel SÁNCHEZ-MORGADO
The National Institute for Medical Research (MRC-NIMR), London,
UK
18:00-18:30
Surgery (post- and pre-operation case) and narcotics
Ignacio ÁLVAREZ
Hospital Universitario La Paz, Cirugía Experimental, Madrid, Spain
18:30-20:00
Dismantle and remove posters and stands
End of the TT2005 Meeting
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TT2005
The TT meetings
The TT2005 meeting is the sixth meeting opportunity for scientists, technicians, students
and commercial exhibitors to meet and discuss different aspects of genetic modification
of animals. These Transgenic Technology (TT) meetings originated in 1998, thanks to
the pioneering initiative of Johannes Wilbertz (Karolinska Center for Transgene
Technologies, Karolinska Institutet, Stockholm) who brought together a range of
important topics which are not usually addressed in other mouse meetings.
That Scandinavian initiative was very well received by the mouse-community, readily
becoming first a European and then finally evolving into one of the key International
meetings where all professionals interested in the production and the analysis of
transgenic animals can meet and openly discuss new methods, their specific problems,
offer solutions and propose imaginative and innovative strategies for the future. The
uniqueness focus of a balanced combination of excellent scientific presentations and
most valuable technical discussions by leading experts in the field are interesting both to
scientists responsible for the analysis of newly created animal models and to qualified
technicians directly engaged in the generation of transgenic animals.
These are the TT meetings organised so far:
TT1998
Karolinska Institute, Stockholm, Sweden
15-16 April 1998
Organisers: Urban Lendahl and Johannes Wilbertz (KI)
TT2000
Karolinska Institute, Stockholm, Sweden
17-19 May 2000
Organisers: Trevor Richards (Cambridge University) and Johannes Wilbertz (KI)
TT2001
Karolinska Institute, Stockholm, Sweden
18-20 June 2001
Organisers: Karolinska Center for Transgene Technologies (KCTT), Johannes Wilbertz
TT2002
Munich, Germany
13-15 October 2002
Organisers: Max-Planck Institute for Biochemistry – Munich, University of Munich - Gene
Center Karolinska Institute – KCTT. Ingrid Renner Müller, Reinhard Fässler, Eckhard Wolf
and Johannes Wilbertz
TT2004
Biomedical Center, Uppsala, Sweden
14-16 March 2004
Organisers: Karin Nilsson, Uppsala University/UUTF, Johannes Wilbertz, Karolinska
Institutet/KCTT, Nelson Khoo, Umeå University/UTCF
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TT2005 Meeting NUMBERS
374 persons are attending the TT2005 meeting (data on 1st September 2005)
297 Standard Participants (79.4%)
33 Invited Speakers (8.8 %)
34 Commercial Delegates (9.1%)
10 Meeting Organisers (2.7 %)
10 Organising or Sponsoring Institutions
23 Sponsoring Companies
37 Invited lectures
92 Posters presented
Country of Origin of TT2005 participants (27 different countries)
Spain
United Kingdom
Germany
USA
France
Sweden
Italy
Switzerland
Hungary
Norway
Netherlands
Canada
Austria
Finland
Japan
Australia
Denmark
Estonia
Greece
Belgium
Brazil
Chile
Cyprus
Taiwan
Israel
Iceland
Portugal
14
135
47
43
26
19
17
12
9
8
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
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Type of Job of TT2005 Participants
Responsible/Head of Transgenic Facility/Unit
Technician in Transgenic Facility/Unit
Postgraduate/PhD. Student
Head of Laboratory
Research Scientist
Postdoctoral Scientist
Head of Department/Unit
Other
Commercial Delegate
Laboratory Technician
Technician in the Animal House
Responsible/Head of the Animal House
Director of Research Unit/Centre
Undergraduate
68
68
52
32
28
22
22
20
18
17
11
9
6
1
Total
374
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