Supplemental File S5. Cell Engineer

Pre-activity Assignment and Assessment
Activity: “Cell Engineers and Cell Detectives”
Post-activity Assessment
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Assessment
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Ask “detectives” to guess the function of the mystery cell, and explain why.
Ask “engineers” to respond.
Encourage discussions and clear up any misconceptions
Mini lecture
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Mesophyll cells (photosynthesis) vs. storage cells
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Goblet cells vs. neuronal cells
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Both have (different types of) plastids
Both have extensive ER/Golgi (one for secretion, one for making membrane)
Clicker activities
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Guess the functions of a series of cell types with specific organelle contents
and structural features
Conclusions and Perspectives
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Linking micro (organelles and cell function) and marco (organism level)
Inspire students to think about bigger questions
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How are cells different (differentiation)
How does one cell (zygote) make different cell types following a spatial and
temporal program (development)
How have all these cell types come into being (evolution)
What does a photosynthetic plant cell look like?
http://www.uri.edu/cels/bio/plant_an
atomy/images/01.gif
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https://faculty.unlv.edu/schulte/Anatomy/Leaves/PrivetLeaf.jpg
Individual cells: high chloroplast content and other features
Working together: difference between two major types of mesophyll cells
What do these cells do?
Avocado
Potato
http://www.emc.maricopa.edu/faculty/farabee/biobk/amyloplast.jpg
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They also contain numerous plastids
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http://nickrentlab.siu.edu/PLB400/Peterson_Images/
Persea_Lipid.jpg
Same type of organelle as chloroplasts
What do they do?
What does a goblet cell look like?
http://www.baileybio.com/plogger/images/anatomy___physiology/12.
_powerpoint_-_digestive_system/goblet_cell.jpg
http://www.bio.davidson.edu/people/kabernd/BerndCV/Lab/Ep
ithelialInfoWeb/goblet cells .jpg
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High abundance of ER and Golgi
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A trans Golgi apparatus cisterna is converted to mucinogen
granules every 3 to 4 minutes during active secretion
What do these cells do?
http://www.columbia.edu/itc/hs/medical/sbpm_histology_old/micrographs/15.jpg
http://cdn.head-fi.org/b/b0/200x200px-ZCb0c4ee5f_ChkNFH-neuron1.jpg
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High abundance of ER and Golgi
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What do they do?
Clicker Time!!
What do these cells do?
Fiber cell in Papyrus
http://emp.byui.edu/wellerg/Cell Types and
Tissues Lab/Images/Fiber.jpg
Stone cells in pears
http://emp.byui.edu/wellerg/Cell Types and
Tissues Lab/Images/Sclerids.jpg
Structural features: dead; very thick secondary cell wall
Function:
a)
b)
c)
d)
e)
Decoration?
Support / protection?
Enlarging surface area?
Water preservation?
Other?
What do these cells do?
http://www.cas.muohio.edu/~meicenrd/anatomy/Ch
7_Protective/pcep.jpg
http://www.unimuenster.de/GeoPalaeontologie/Palaeo/Palbot/c
ut1a.jpg
Structural features: Tightly packed together;
thick waterproof cuticle layer
Function:
a)
b)
c)
d)
e)
Decoration?
Support / protection?
Enlarging surface area?
Water preservation?
Other?
What do these cells do?
http://upload.wikimedia.org/wikipedia/c
ommons/7/75/Arabidopsis-epidermand-trichome.jpg
http://www.queensu.ca/biology/new
s/sneddentrichome/trichome.gif
http://t1.gstatic.com/images?q=tbn:ANd9G
cRrA2tze02y1uajzmDCObWYYXfhnL4Cu
FIe72KwKcEINNGW47wq
Structural features: sharp outgrowth
Function:
a)
b)
c)
d)
e)
Decoration?
Support / protection?
Enlarging surface area?
Water preservation?
Other?
http://www.namethatplant.net/Images/Ima
gesTN/tn_mam/tn_mam_roughleafdog06
0310dz.jpg
What do these cells do?
http://botit.botany.wisc.edu/Resources/Botany/
Root/Grass Root Tip/root hair.jpg
http://www.bio.miami.edu/d
ana/pix/roothairs.jpg
Structural features: more delicate outgrowth
Function:
a)
b)
c)
d)
e)
Decoration?
Support / protection?
Enlarging surface area?
Water preservation?
Other?
http://www.amjbot.org/co
ntent/90/6/823/F3.large.j
pg
What do these cells do?
http://lh5.ggpht.com/_wYv4UjyptOQ/TPMRfco0LpI/AAAA
AAAAAzA/uJMDI2XWDr0/image131_thumb.png?imgmax
=800
http://discoverysedge.mayo.edu/diabetesmetabolism/images/de10-4-diabetes-myofibril.jpg
Structural features: numerous mitochondria & myofibrils
Function:
a)
b)
c)
d)
e)
Decoration?
Support / protection?
Enlarging surface area?
Water preservation?
Other?
Summary
• What have you learned today?
– Relationship between organelle content, cell
structure and cellular functions
• What new questions do you have now?