CK40-RFL

INSTRUCTIONS
CK40-RFL
REFLECTED LIGHT ATTACHMENT
This instruction manual is for the Olympus CK40-RFL Reflected Light Attachment.
To ensure the safety, obtain optimum performance and familiarize yourself fully
with the use of this product, we recommend that you study this manual thoroughly
before operating the equipment. Retain this instruction manual in an easily accessible place near the work desk for future reference.
AX7170
IMPORTANT
This attachment employs an LB optical design, and should be used only with equipment using
the LB optical design, such as the CK40 microscope frame and its eyepiece, objective and condenser. Less than optimal performance may result if inappropriate accessories are used.
SAFETY PRECAUTIONS
1. Make sure that the main switch on the power supply unit is set to “\” (OFF) before connecting the
power cord to the AC mains.
2. The power supply unit contains high voltage components. Never attempt to disassemble the unit, or
you may run the risk of electric shock.
3. Always be sure to ground (earth) the equipment. Otherwise Olympus will no longer warrant the electrical
safety of the equipment.
4. The attachment should be installed on a flat surface so that the ventilation opening on the bottom
panel is not blocked. Do not install the attachment on a surface which is soft or which may collapse
under the weight of the microscope, for the subsequent blockage of the bottom panel ventilation
opening could create a fire hazard.
5. The high pressure mercury burner (mercury arc lamp) used should be a HBO50W/AC (OSRAM) or
CS50W4 (PHILIPS) lamp.
6. Before opening the lamp housing for replacement of the burner or any other internal part, set the main
switch to “\” (OFF), unplug the lamp housing´s connecting cord plug from the output connector on the
power supply unit, and wait 10 minutes or more until the lamp housing cools down.
7. Do not open the lamp housing while it is turned on or for at least 10 minutes after it has been turned off.
Lamp housing parts are extremely hot and cause burns if touched. (See page 9.)
8. Do not remove the lamp housing while the burner is lit. Also, do not turn the burner on while the lamp
housing is removed.
9. Do not install the lamp housing upside down or at an angle. The lamp housing surface becomes very
hot during operation, so ensure that there is ample free space around the lamp housing, especially
above and below.
10. If the microscope power cord comes in contact with the lamp housing or its surroundings, the cord
may melt and subsequently cause electric shock. Be sure to position the power cord at a safe distance
from the lamp housing.
11. A used mercury burner should be disposed of in compliance with the ordinances or regulations of your
national or local government.
12. To replace the lithium battery (for the hour counter) built into the power supply unit, consult your local
Olympus representative. As the power supply unit incorporates a lithium battery, it should be disposed
of in the same way as a used mercury burner, i.e., in compliance with the ordinances or regulations of
your national or local government.
13. Before placement of the dust cover for protection after operation, wait until the lamp housing cools
down sufficiently and unplug the power cord.
i
Safety Symbols
The following symbols are found on the equipment. Study the meaning of the symbols, and always use the
equipment in the safest possible manner.
Symbol
Explanation
Indicates that the surface becomes hot, and should not be touched with
bare hands.
Before use, carefully read the instruction manual. Improper use could
result in personal injury to the user and/or damage to the equipment.
Indicates a potential fire hazard; when replacing fuses, be sure replacement fuses are of the specified rating.
I
\
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Warning Indications
Warning indications are placed at parts where special precaution is required when handling and using the
equipment. Always heed the warnings.
Warning indication position
1
Lamp housing [Warning against high temperature]
Getting Ready
1. This attachment is a precision instrument. Handle it with care and avoid subjecting it to sudden or
severe impact.
2. Do not use the attachment where it is subjected to direct sunlight, high temperature and humidity, dust
or vibrations. (For operating environment conditions, see Section 7, “SPECIFICATIONS” on page 20.)
3. Make certain that the burner is installed correctly and that all cords are correctly connected.
4. Do not apply excessive force to the limiting mechanism provided with all functions.
ii
2
Maintenance and Storage
1. Be careful to avoid leaving dirt or fingerprints on the lenses, filters or high pressure mercury burner, for
contamination of these parts may cast the shadow of a foreign object in the field of view or compromise
burner performance. If contaminated, clean by wiping gently with a piece of gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol
(30%), EE System Cleaner (Olympus EE-6310), alcohol or xylene.
Since ether, alcohol and EE System Cleaner are highly flammable, be careful to keep these
chemicals away from open fire and potential sources of electrical sparks, such as sparks from
main switches switching ON or OFF.
2. The mercury burner has an average service life of 100 hours. When the hour counter on the power
supply unit indicates 100 hours, replace the burner with a new one (see page 14). Using a burner after its
service life expires could lead to the burner exploding, though this occurs very rarely.
3. If a dichroic mirror cube is not going to be used for a while, place it in its container and store it in a safe
place.
4. The dichroic mirror cube surface is very delicate. When it becomes necessary to clean it, please consult
us or your local Olympus representative.
5. Never disassemble any part of the attachment, for this may degrade performance.
3
Caution
If the equipment is used in a manner not specified by this manual, the safety of the user may be imperiled.
In addition, the equipment may also be damaged. Always operate the equipment as outlined in this
instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily
harm to the user and/or damage to equipment (including objects in the vicinity of the
equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).
iii
CK40-RFL
CONTENTS
1
PRINCIPLE
1
2
NOMENCLATURE
2
3
ASSEMBLY
3
3-1 Assembly Diagram ............................................................................................................................. 3
3-2 Detailed Assembly Procedure ........................................................................................... 4
4
CONTROLS OF EACH MODULE
12
5
PREPARATION BEFORE OBSERVATION
14
6
OBSERVATION
19
7
SPECIFICATIONS
20
8
TROUBLESHOOTING GUIDE
21
■ PROPER SELECTION OF POWER SUPPLY CORD ..................................... 23
PRINCIPLE
The design of the reflected light fluorescent microscope features a dichroic mirror* which directs the excitation
light through the objective to illuminate the specimen and provide efficient fluorescent light observation.
Specimen
Emitted light
Objective
Excitation light
Dichroic mirror
Collector
Light source
Exciter filter
Barrier
filter
Eyepiece
* The dichroic mirror enables efficient fluorescent light microscopy by making use of its characteristics of short
wavelength reflection and long wavelength transmission.
Reflectance
Absorbance
50%
1
The spectral characteristics of the dichroic mirror when it is
positioned at an inclination of 45° to the optical axis of incident light is shown in the figure on the left. Because crossover
exists between transmittance and reflectance, it is necessary
Transmittance
to employ an appropriate combination of exciter and barrier
filters in conjunction with the dichroic mirror to obtain a wellcontrasted image.
Fluorescence
When the dichroic mirror is inclined at 45° to the optical axis
of the incident excitation light, the excitation light is reflected
towards the objective and other unwanted wavelengths pass
through the mirror, practically in their entirety.
When the specimen is irradiated by the excitation wavelength,
it emits a visible, longer wavelength in accordance with Stokes´
λM
Wavelength Law. The dichroic mirror passes almost all of this light to the
eyepiece. The barrier filter mounted between the dichroic mirCrossover
ror and the eyepiece blocks out unwanted wavelengths to
provide a black background.
CK40-RFL
NOMENCLATURE
Excitation light
protective shield
Fluorescent vertical illuminator
CK40-RFA
Lamp housing
U-LH50HG
Microscope
CK40
Fluorescent mirror cubes
CX-DMB-2
CX-DMG-2
Power supply unit
U-RFLT50
2
ASSEMBLY
3-1 Assembly Diagram
The diagram below shows how to assemble the various modules for reflected light fluorescence observation.
The numbers in the diagram indicate the assembly step order.
# When assembling the microscope system, make sure that all parts are free of dust and dirt, and avoid
scratching any parts or touching the glass surfaces.
Lamp housing
U-LH50HG
Mercury
burner
Fluorescent vertical
illuminator
CK40-RFA
Cover
Power cord
Microscope
CK40
Excitation light
protective shield
Power supply unit
U-RFLT50
RCUBE indication
Light shield cap*
Right light excluding plate
Cube
Removal lever
Right cover
Left cover
Required Tool
Removal lever
Cube
LCUBE indication
Allen wrench
Left light
excluding plate
* The light shield cap is provided only with the left cover.
3
CK40-RFL
3-2 Detailed Assembly Procedure
1
|
²
³
@
Fig. 1
…
Mounting the Fluorescent Vertical
Illuminator (CK40-RFA)
(Figs. 1 - 3)
1. Set the main switch of the microscope (CK40) to “\” (OFF).
For safety, unplug the power cord @ until the fluorescent
vertical illuminator has been mounted completely. (Fig. 1)
2. Unplug the lamp cord socket ² of the illumination support
because it gets in the way of removing the cover ³. (Fig. 1)
3. Move the revolving nosepiece to the upper limit by turning
the coarse and fine focus adjustment knobs.
4. Pull out the cover ³ by hooking a finger into the removal
hole |. (Fig. 1)
5. Loosen one of the filter slider knobs ƒ and pull out the filter
slider …. (Fig. 2)
ƒ
Fig. 2
6. Insert the fluorescent vertical illuminator † gently into the
cover removed above, and tighten the provided clamping
screws (x 3) securely using the Allen wrench. (Fig. 3)
7. Mount the filter slider to the original position and attach the
filter slider knob.
†
Fig. 3
4
2 Mounting the Fluorescent Mirror Cubes
(Figs. 4 - 8)
} 2-wavelength observation using excitations B and G or transmitted-light brightfield observation using one excitation can
be selected according to mounted cubes. Mount cubes to
meet the desired observation type.
# While the CX-DMB-2 and CX-DMG-2 fluorescent mirror
cubes can be mounted either on the left or right, the
light excluding plates and cube covers for the left and
right are provided with different designs. Check the assembly diagram to ensure design and orientation before using these parts.
# The cube covers must always be mounted, for they are
important for glare protection during cube switching and
cube positioning.
Right cube
1. Place a cube @ as shown in Fig. 4.
2. Orient the right light excluding plate as shown in Fig. 4, and
using the Allen wrench, fit the stopper pin ³ into the mounting threaded hole | on the cube.
³
²
@
|
Fig. 4
3. Place the cube with the right light excluding plate in the
normal position shown in Fig. 5, and insert it gently into the
dovetail ƒ on the illuminator so that it is not inclined.
# Be careful that the cube is oriented right side up. The
cube cannot be inserted if it is held upside down.
ƒ
Fig. 5
5
CK40-RFL
4. Mount the right cover … by aligning the clamping screws †
with the threaded holes ‡ and tightening using the Allen
wrench. (Fig. 6)
…
‡
†
Fig. 6
Š
‰
Œ
5. Insert the cube installation/removal lever Š, which is marked
for use with the cube mounted on the right, into the lever
hole ‰, and tighten the lever in the mounting threaded hole
on the cube. (Fig. 7)
6. Orient the indication plate ‹ of the cube installation/removal
lever in the forward direction by loosening the indication
plate clamping knob Œ, then tightening it.
‹
Fig. 7
<<Removing the cube installation/removal lever>>
If the cube installation/removal lever cannot be removed by
turning it counterclockwise, remove the indication plate
clamping knob and turn the internal screw counterclockwise using a flat-blade screwdriver.
Left cube
1. In the same way as for the right cube, place the left cube as
shown in Fig. 8 and mount the left light excluding plate onto
it.
2. Remove the light shield cap on the left cover by pushing it
outward.
# When only the left cube is mounted, re-attach the light
shield cap onto the right cover.
3. In the same way as for the right cube, mount the left cube on
the side opposite to the right cube and attach the left cover
and cube installation/removal lever.
Fig. 8
6
3
²
Mounting the Excitation Light Protective
Shield
(Fig. 9)
Insert the excitation light protective shield @ from the bottom of the condenser ² until it stops, then tighten the clamping screw ³.
The wider end of the protective shield should be on the
front side of the microscope.
@
³
Fig. 9
4 Mounting the U-LH50HG Lamp Housing
²
³
ƒ
@
Fig. 10
7
|
(Fig. 10)
1. Insert the mercury burner housing collector @ into the
vertical illuminator until it reaches the click-stop.
2. Turn the lamp housing so that its top panel ² becomes
horizontal, then tighten the clamping screw ³ (x 1) on the
illuminator using the Allen wrench.
As the lamp housing surface becomes very hot during
operation, ensure that there is ample free space around
the lamp housing, especially above and below.
3. Plug the CK40 power cord | and the lamp cord socket ƒ
(removed as above) into the original positions.
If the CK40 power cord comes in contact with the lamp
housing or its surroundings, the cord could melt and
cause electric shock. Be sure to position the power cord
at a safe distance from the lamp housing.
CK40-RFL
5
Mounting the High Pressure
Mercury Burner
(Figs. 11 - 14)
<<Applicable burners>>
· HBO50W/AC (OSRAM)
· CS50W4 (PHILIPS)
@
Fig. 11
²
|
³
Fig. 12
# Burner type information must be set in the power supply unit, so check the burner box or burner base to confirm whether it is of type L1 or L2.
1. Using the Allen wrench, loosen the burner socket clamping
screw @ and flap open the burner socket section by about
90° in the direction of the arrow. (Fig. 11)
2. Loosen the burner clamping screws ² and ³ and remove
the transport post attached to the burner socket.
}To replace the burner, remove the used burner. (Fig. 12)
3. Loosen the clamping screw ² and attach the extremity without the “UP” marking of the mercury burner | so that it
comes on the lower side. Then, loosen the clamping screw
³ and attach the extremity marked “UP”. (Fig. 12)
# Do not mount the mercury burner upside down. Otherwise the light may become dim, the original performance
will not be achievable, and burner service life may be
shortened.
# Contamination of the burner surface with dirt may cause
its shadow to be cast in the field of view. Be sure to
remove any dirt from the burner surface.
4. Close the burner socket with burner into the original position and tighten the socket clamping screw @. (Fig. 11)
}A click sound is generated when the socket clamping screw
@ is tightened. This sound indicates the normal action of
an interlock switch safety mechanism. (Fig. 11)
8
ƒ
Fig. 13
5. Set the burner type switch ƒ on the rear of the power
supply unit according to the type of the mounted burner
(L1 or L2). (Fig. 13)
# If the clamping screw @ is accidentally loosened while
the burner is operating, the interlock switch turns off the
burner. (Fig. 11)
To restart the burner, first set the main switch on the power
supply unit to “\” (OFF) and wait about 10 minutes*. Then,
after firmly tightening the clamping screw @, set the main
switch to “ l ” (ON) again.
* The burner cannot be re-ignited for about 10 minutes; that
is, until the mercury vapor inside it has cooled down and
condensed to liquid.
Caution for burner replacement during observation
The burner, lamp housing and its surroundings are very
hot during operation.
Before replacing the burner, set the main switch to “\”
(OFF), unplug the power cord and wait until the burner,
lamp housing and its surroundings cool down completely.
…
Fig. 14
9
6. Press the reset button … on the front of the power supply
unit to reset the hour counter display to “0.00”. (Fig. 14)
} The hour counter display registers hour units. Replace the
lamp when the displayed time reaches “100.00” hours.
Note that the service life of a mercury burner may expire
before the above-mentioned average depending on how
frequently the “ l ” (ON) and “\” (OFF) switch is used. When
start-up of the burner degrades or flickering becomes noticeable, replace the burner regardless of the hour counter
indication.
# Be sure to reset to “0.00”. Otherwise the burner may
not be ignitable.
Clear more than 10 cm of space around the power
supply unit to assure proper heat radiation.
The power supply unit should be installed so that the
main switch can easily be accessed and set to “\” (OFF)
in case of emergency.
CK40-RFL
6 Setting Input Power Voltage
²
@
(Fig. 15)
1. Using a flat-blade screwdriver, set the voltage selector switch
@ on the rear of the power supply unit to your local power
voltage.
2. Set the frequency switch ² to your local power frequency.
# Improper setting of the above items may degrade burner
performance, or in the worst (although very rare) case,
cause the burner to explode.
Fig. 15
7 Connecting the Cords
|
²
³
@
Fig. 16
(Figs. 16 - 18)
Do not forcefully bend or twist the cords. They are sensitive to such application of force, which can cause internal wire disconnection and short-circuiting.
Be sure to set the main switch on the power supply unit
to “\” (OFF) before connecting the cords.
1. Plug the connector @ from the burner socket securely into
the connector ² on the power supply unit. (Fig. 16)
Always use the U-LH50HG Lamp Housing.
Always use the power cord provided by Olympus. If no
power cord is provided, please select the proper power
cord by referring to the section “PROPER SELECTION
OF THE POWER SUPPLY CORD” at the end of this instruction manual.
2. Plug the connector ³ of the power cord securely into the
connector | on the power supply unit. (Fig. 16)
10
ƒ
Fig. 17
…
3. Insert the power cord plug ƒ into the wall outlet ….
(Fig. 17)
Connect the power cord correctly and ensure that the
ground terminal of the power supply and that of the wall
outlet are properly connected. If the equipment is not
grounded, Olympus can no longer warrant the electrical
safety and performance of the equipment.
If the power cord or burner socket cord comes in contact with the lamp housing or its surroundings, the cord
may melt and cause electric shock. Be sure to position
the power cord and burner socket cord at a safe distance from the lamp housing.
Fuse replacement
†
Fig. 18
# Set the main switch to “\” (OFF) and unplug the power
cord before replacing fuses.
1. Remove each of the fuse holders † by turning it counterclockwise using a flat-blade screwdriver and pulling
out. (Fig. 18)
2. Replace both fuses with new ones.
# Make sure that the replacement fuses are of the properly
specified rating. Otherwise a fire hazard may result.
U-RFLT50-100: T4A(H)250V
(LITTELFUSE 215004), 2 pieces
Applicable fuses
U-RFLT50-200: T2.5A(H)250V
(LITTELFUSE 21502.5), 2 pieces
11
CK40-RFL
CONTROLS OF EACH MODULE
Fluorescent Vertical Illuminator CK40-RFA
Field iris diaphragm lever
Open: Clockwise
Close: Counterclockwise
Field iris centering knob
Filter slider
Shutter/Open aperture/Filter pocket
· Accommodates the ND filter.
Cube installation/removal lever
· B excitation mirror cube lever
· G excitation mirror cube lever
Index groove
Fluorescent mirror cubes
· B excitation: CX-DMB-2
· G excitation: CX-DMG-2
ND Filter (32ND25)
12
Lamp Housing U-LH50HG
Collector focusing
knob
Burner up/down
centering knob
Rear
Mirror left/right
centering screw
Mirror focusing
screw
Mirror up/down
centering screw
Burner left/right
centering knob
Power Supply U-RFLT50
# The depth dimensions of the 100 V and 200 V models
differ from each other.
Frequency
switch
Reset button
Hour counter
Voltage
switch
Burner type
switch
Fuse
holders
Power cord
connector
Main switch
I : ON
\: OFF
13
Starter reset
switch
Lamp housing
connector
For exclusive use with
the U-LH50HG.
CK40-RFL
PREPARATION BEFORE OBSERVATION
1 Turning ON the Mercury Burner
@
Fig. 19
(Fig. 19)
# Confirm that the power supply unit has been set to the
proper power supply voltage, power supply frequency
and burner type. Improper setting of these items may
degrade burner service life considerably.
· Set the main switch on the power supply unit to “ I ” (ON).
After the burner is ignited, between 5 and 10 minutes are
required before the arc stabilizes.
# Some mercury burners may not ignite the first time the
power is turned ON, and the safety mechanism in the
starter of the power supply unit is activated to reset the
starter in such a case. If this occurs, set the main switch
to “\” (OFF) once, then insert a thin object such as a
ballpoint pen tip into the starter reset hole @ on the
right panel of the power supply unit to press the internal
switch, and set the main switch to “ I ” (ON) again.
# To avoid shortening the power supply unit´s operating
service life, avoid turning power on while the burner is
not mounted, or turning power on and off within short
time intervals.
# To avoid shortening burner life, do not turn the burner
off within 15 minutes after ignition. Use the shutter
Instead.
# After the burner is turned off, it cannot be re-ignited before the mercury vapor cools and condenses to liquid.
Wait about 10 minutes before restarting the burner.
If the lamp housing is opened while the burner is ignited, the safety interlock will activate and switch off the
power automatically. In this case, set the main switch to
“\” (OFF) and wait for more than 10 minutes before restarting the burner. The lamp housing can be opened
only after it has cooled down fully.
When resetting the hour counter, be sure to press the
reset button until “0.00” is displayed.
When the hour counter indicates “100.00”, set the main
switch to “\” (OFF) for safety, wait for more than 10 minutes, then replace the lamp burner after making sure
that the housing has cooled down.
A mercury burner seals high-pressure gas inside. If the
burner is used beyond its service life, stress may accumulate inside the burner, and in the worst (but very rare)
case, the burner could explode.
14
2 Centering the Mercury Burner
²
@
³
Fig. 20
|
ƒ
Fig. 21
15
(Figs. 20 - 24)
}Before attempting to center the burner, wait for the arc image to stabilize. To protect against glare during arc image
centering, it should be viewed across the excitation light
protective shield (see page 7).
1. Remove the cap (or objective) from the revolving nosepiece
and engage the open aperture into the light path.
2. Push the “B” cube installation/removal knob @ to engage it
into the light path. (Fig. 20)
3. Turn the field iris diaphragm lever ² clockwise to open the
iris diaphragm. (Fig. 20)
4. Place a piece of thin white paper, such as copy paper, on
the top of the stage, set the filter slider ³ to the central
position, open aperture position, and project the arc image.
(Fig. 20)
}The arc image can be confirmed more easily by using the
U-CST centering target screen.
# If the arc image is too bright, insert the ND filter | into
the filter pocket ƒ and engage the filter pocket into the
light path. (Fig. 21)
32ND25: Reduces transmittance to 25%.
# If the arc image focusing position cannot be identified
easily, close the iris diaphragm.
CK40-RFL
Š
‹
‰
…
‡
†
Fig. 22
Terminal
Arc image
Terminal
Fig. 23
¬
Mirror reflected
image
®
5. Project the arc image on the white paper, then center and
sharpen it by manipulating the collector focusing knob …,
burner left/right centering knob † and burner up/down centering knob ‡ on the lamp housing. (Figs. 22 & 23)
# There may be cases in which the arc image projected
on the white paper is a mirror reflected image. If the arc
image moves when the mirror left/right centering screw
Š or mirror up/down centering screw ‰ is turned, it is a
mirror reflected image; bring it out of the field and center the arc image as described above. (Fig. 22)
6. Manipulate the burner left/right centering knob † slightly to
move the arc image to the right (or left). (Figs. 22 & 24¬)
7. Using the Allen wrench provided with the vertical illuminator,
turn the mirror focusing screw ‹, mirror left/right centering
screw Š and mirror up/down centering screw ‰ to form the
mirror reflected arc image on the white paper. (Figs. 22 & 24®)
8. Adjust the mirror centering screws Š and ‰ to move the
direct and mirror reflected images in the symmetrical position between each other, and turn the mirror focusing screw
‹ to adjust the size of the mirror reflected image until it is
identical to that of the direct image. (Figs. 22 & 24¯)
9. Turn the burner left/right centering screw † to overlap the
direct image with the mirror reflected image. (Figs. 22 & 24¶)
10. Engage the 10X objective into the light path while continuing to use the “B” excitation mirror cube.
11. Place a specimen on the stage and bring it into approximate focus.
12. Look into the eyepiece and manipulate the collector focusing knob … to make the field as bright and regular as possible, then turn the knob clockwise to tighten. (Fig. 22)
}Maintain this condition until the next time the burner is replaced.
¯
¶
Fig. 24
16
3 Centering the Field Iris Diaphragm
@
²
Fig. 25
(Figs. 25 & 26)
1. Engage the 10X objective into the light path, push the “B”
cube installation/removal knob into the light path, and set
the filter slider to the central open aperture position or the
position of the filter pocket accommodating the ND filter.
2. Place a specimen on the stage and bring into approximate
focus.
3. Turn the field iris diaphragm lever @ counterclockwise,
little by little, and confirm the iris diaphragm position.
(Figs. 25 & 26)
4. Manipulate the 2 field iris centering knobs ² to move the
image of the diaphragm to the center. (Figs. 25 & 26)
5. After moving the image to the center, open the field iris diaphragm. As this makes slight deviation noticeable, adjust
the centering precisely.
6. After completion of centering, enlarge the iris diaphragm diameter until it just circumscribes the field of view.
Adjusting the field iris diaphragm
Fig. 26
17
To obtain good image contrast, adjust the diameter of the
illuminating beam, according to the objective in use. The
field iris diaphragm can be adjusted only within the required
area for observation, to prevent fading of specimen areas
outside the observed field.
According to the objective in use, adjust the diaphragm
image using the field iris diaphragm lever so that the field of
view is circumscribed by the field iris diaphragm to exclude
stray light.
CK40-RFL
4 Selecting Mirror Cubes
}Select the mirror cube which matches the purpose of observation.
Cube Dichroic Mirror/Filter Combinations
Excitation Mirror Cube
B
CX-DMB-2
G
CX-DMG-2
Dichroic Mirror
DM500
DM570
Exciter Filter
BP460-490
BP475-545
Barrier Filte
Applicable Reagents
OG515IF
FITC
Acridine orange
Auramine
GFP
OG590
Rhodamine B200
TRITC
Feulgen
Propidium iodide
Filter Characteristics
G excitation (Super wide band)
B excitation (Wide band)
Spectral Transmittance (%)
Spectral Transmittance (%)
100
DM500
BP460-490
OG515IF
50
0
300
400
500
600
Wavelength (mm)
700
800
100
BP475-545
DM570
BP475-545
50
OG590
0
300
400
500
600
700
800
Wavelength (mm)
18
OBSERVATION
}The excitation light from parts other than the excitation light protective shield may be glaring to your eyes.
However, this light is not hazardous because a UV cut filter is built into the lamp housing.
# The objectives provided with the CK40 microscope can be used for fluorescent observation. However, when the phase contrast objective or relief phase contrast objective is used, the image may
become slightly dark or the contrast may become degraded, due to the built-in phase plate.
ƒ
³
|
²
Fig. 27
@
1. Pull out the filter slider @ fully toward the right side to
engage the shutter into the light path. (Fig. 27)
2. Pull the left and right cube installation/removal levers ² fully
outward to remove the cubes from the reflected fluorescent
light path. (Fig. 27)
# Pull out each lever until the index groove ³ at its deeper
end is visible. (Fig. 27)
3. Perform transmitted light observation of the CK40 and move
the specimen section to be observed to the center of the
field of view.
}The transmitted light observation can be either phase contrast observation or brightfield observation. If a specimen is
hard to observe in brightfield observation, adjust the condenser aperture iris toward the closing direction.
4. Turn off the transmitted light, push in the installation/removal
lever of the fluorescent mirror cube to be used in observation
(until the index groove | on the lever end is aligned with the
side panel ƒ of the cover), slide the filter slider to disengage
the shutter from the light path, and perform fluorescent observation. (Fig. 27)
}Adjust the collector focusing knob on the lamp housing
to make the entire field of view uniform and as bright as
possible.
}Adjust the field iris diaphragm lever so that the field of view
is circumscribed by the field iris diaphragm.
}To interrupt observation for a short period, use the shutter
and keep the burner ignited.
Repeated switching ON and OFF of the burner will shorten
its service life.
Caution for fluorescence observation
This unit provides maximum excitation intensity to enable image observation even of specimens with low fluorescence. Therefore, during observation with a high-power objective, fading
may occur, resulting in loss of image brightness and contrast.
To prevent this, reduce the intensity of the excitation light to reduce the speed of fading. It is
advisable that the intensity of the excitation light is reduced by using an ND filter. Also, use the
shutter so that the specimen will not be exposed to excitation light for longer than is necessary.
By using a commercially available anti-fading agent (e.g., DABCO), fading of the specimen can
be slowed. For high-magnification observation, in particular, use of an anti-fading agent is recommended.
# Note that anti-fading agents are not applicable to some specimens.
19
CK40-RFL
SPECIFICATIONS
Module
Specification
Fluorescent vertical illuminator
CK40-RFA
Fluorescent lighting method: Reflected fluorescent lighting based on
slide switching of dichroic mirror cubes.
Number of fields of view: 20 (when WHK10X is used).
Intermediate tube magnification: 1X.
Shutter: Switchable using filter slider (shutter/open aperture/filter
pocket).
Excitation protective shield: Fixed on condenser with a screw.
Possible observation modes: B excitation/G excitation.
Lamp housing
U-LH50HG
Burner centering: Up/down, left/right.
Mirror centering: Up/down, left/right.
Burner focusing: Collector focusing, mirror focus, screw system.
Safety mechanism: Interlock switch, UV cut filter built in.
<<Applicable high-pressure mercury burners>>
· HBO50W/AC (OSRAM)
· CS50W4 (PHILIPS)
Power supply
U-RFLT50-100
U-RFLT50-200
Ignition system: Auto ignition.
Hour counter: Displays accumulated hours of operation.
Input rating: Input voltage switchable.
U-RFLT50-100: 100/110/120 V AC
, 2.5/2.3/2.0 A, 50/60 Hz.
U-RFLT50-200: 220/230/240 V AC
, 1.7 A, 50/60 Hz.
Dimensions
U-RFLT50-100: 150(W) x 320(D) x 150(H) mm.
U-RFLT50-200: 150(W) x 210(D) x 150(H) mm.
Weight
U-RFLT50-100: 12 kg (26.4 lb.).
U-RFLT50-200: 7 kg (15.4 lb.).
Operating environment
Indoor use.
Altitude: Max. 2000 m.
Ambient temperature: 5°C to 40°C. (41°F to 104°F)
Maximum relative humidity: 80% for temperatures up to 31°C (88°F)
decreasing linearly through 70% at 34°C
(93°F), 60% at 37°C (99°F), to 50% relative
humidity at 40°C (104°F).
Supply voltage : Fluctuations not to exceed ±10% of nominal voltage.
Pollution degree: 2 (in accordance with IEC664).
Installation/overvoltage category: II (in accordance with IEC664).
20
TROUBLESHOOTING GUIDE
Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If
problems occur, please review the following list and take remedial action as needed. If you cannot solve the
problem after checking the entire list, please contact your local Olympus representative for assistance.
Problem
Cause
Remedy
Page
ND filter is engaged.
Set filter slider to open aperture
position.
15
Cube is not engaged properly
in light path.
Engage it properly in light path.
Shutter is engaged in light path.
Set filter slider to open aperture
position.
15
The wrong 40X objective is
mounted, or the objective’s correction collar has not been adjusted.
Optimum fluorescence performance cannot be achieved with
a 40X objective that does not
have a correction collar. Use the
LWDCDPlan40XPL-6 or
LWDCDPlan40X with correction
collar and adjust the collar.
–
Objectives and/or filters are dirty.
Clean them.
iii
Field iris diaphragm is not correctly opened.
Adjust field iris diaphragm so
that its image circumscribes the
field of view.
17
Objective is not engaged properly in light path.
Be sure to stop the revolving
nosepiese at a click position.
–
Cube is not engaged properly
in light path.
Engage it properly in light path.
Filter slider is located in the
middle of light path.
Set filter slider to open aperture
position.
ND filter is located in the middle
of light path.
Set it to proper position.
Mercury burner is not centered
properly.
Center mercury burner correctly.
Collector focus deviates from
correct position.
Adjust to correct position.
Power cord is not connected
properly.
Connect correctly.
A fuse is blown.
Replace it.
1. Optical System
a) Mercury burner lights, but field
of view remains dark.
b) Image is unclear, blurred or
has insufficient contrast.
c) Field of view is vignetted, or it
is not evenly illuminated.
19
19
15
15
15
16
2. Electrical System
a) Main switch indicator does
not light up.
21
10
11
CK40-RFL
Problem
Cause
b) Main switch indicator lights,
but the mercury burner does
not ignite.
Connectors are not connected
properly.
Connect correctly.
Mercury burner is not mounted.
Install a burner.
8
Lamp housing interlock switch
is activated.
Tighten the burner socket
clamping screw.
9
Auto ignition is malfunctioning.
Set main switch on the power
supply unit to “\” (OFF), then
set to “ l ” (ON) again. (Repeat
as necessary.)
14
Push the switch inside the starter
reset hole on the right side of
power supply unit, then set main
switch to “ l ” (ON) again.
14
Wait for at least 10 minutes after
re-igniting the burner.
14
c) Mercury burner flickers or is
dark.
Auto ignition safety mechanism
is activated.
Insufficient time has elapsed
since the burner was turned on.
d) Mercury burner life is very
short.
Remedy
Page
10
Input voltage and frequency Set them correctly.
switches are not set properly.
10
Burner service life has expired.
Replace the mercury burner.
8
Input voltage is not set properly.
Set correctly.
10
This device complies with the requirements of both directive 89/336/EEC concerning
electromagnetic compatibility and directive 73/23/EEC concerning low voltage. The CE
marking indicates compliance with the above directives.
22
■ PROPER SELECTION OF THE POWER SUPPLY CORD
If no power supply cord is provided, please select the proper power supply cord for the eqipment by referring
to “ Specifications ” and “ Certified cord ” below:
CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can
no longer warrant the electrical safety of the eqipment.
Specifications
Voltage Rating
Current Rating
Temperature Rating
Length
Fitting Configuration
125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
6A minimum
60°C minimum
3.05 m maximum
Grounding type attachment pulg cap. Opposite terminates in molded-on
IEC configuration appliance coupling.
Table 1 Certified Cord
A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage
marked with an agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least
one of agencies listed in Table 1. In case you are unable to buy locally in your country the power supply cord
which is equivalent and authorized agencies in your country.
Country
23
Agency
Certification
Mark
Country
Agency
Australia
SAA
Italy
IMQ
Austria
ÖVE
Japan
MITI
Belgium
CEBEC
Netherlands
KEMA
Canada
CSA
Norway
NEMKO
Denmark
DEMKO
Spain
AEE
Finland
FEI
Sweden
SEMKO
France
UTE
Switzerland
SEV
Germany
VDE
United
Kingdom
ASTA
BSI
Ireland
NSAI
U.S.A.
UL
Certification
Mark
CK40-RFL
Table 2 HAR Flexible Cord
APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS
Approval Organization
Printed or embossed Har- Alternative Marking Utilizing
monization Marking (May be Black-Red-Yellow Thread (Length
located on jacket or insula- of color section in mm)
tion of internal wiring)
Black
Red
Yellow
Comite Electrotechnique Belge
(CEBEC)
CEVEC
<HAR>
10
30
10
Verband Deutscher Elektrotechniker
(VDE) e.V. Prüfstelle
<VDE>
<HAR>
30
10
10
Union Technique de d´Electricite´
(UTE)
USE
<HAR>
30
30
10
Instituto Italiano del Marchio di
Qualita´ (IMQ)
IEMMEQU
<HAR>
10
30
50
British Approvals Service for Electric
Cables (BASEC)
BASEC
<HAR>
10
10
30
N.V. KEMA
KEMA-KEUR
<HAR>
10
30
30
SEMKO AB Svenska Elektriska
Materielkontorollanstalter
SEMKO
<HAR>
10
10
50
Österreichischer Verband für
Elektrotechnik (ÖVK)
<ÖVE>
<HAR>
30
10
50
Danmarks Elektriske Materielkontrol
(DEMKO)
<DEMKO>
<HAR>
30
10
30
National Standards Authority of Ireland
(NSAI)
<NSAI>
<HAR>
30
30
50
Norges Elektriske Materiellkontroll
(NEMKO)
NEMKO
<HAR>
10
10
70
Asociacion Electrotecnica Y
Electronica Espanola (AEE)
<UNDE>
<HAR>
30
10
70
Hellenic Organization for
Standardization (ELOT)
ELOT
<HAR>
30
30
70
Instituto Portugues da Qualidade
(IPQ)
l np l
<HAR>
10
10
90
Schweizerischer Elektro
Technischer Verein (SEV)
SEV
<HAR>
10
30
90
Elektriska Inspektoratet
SETI
<HAR>
10
30
90
Underwriters Laboratories Inc. (UL)
Canadian Standards Association (CSA)
SV, SVT, SJ or SJT, 3 X 18AWG
SV, SVT, SJ or SJT, 3 X 18AWG
24
MEMO
43-2, Hatagaya 2-chome, Shibuya-ku, Tokyo Japan
(Premises/Goods delivery) Wendenstasse 14-16, D-20097 Hamburg, Germany
(Letters) Postfach 10 49 08, 20034 Hamburg, Germany
2 Corporate Center Drive, Melville, N.Y. 11747-3157, U.S.A.
2-8 Honduras Street, London EC1Y OTX, United Kingdom
This publication is printed on recycled paper.
Printed in Japan 9712 M 020–@