Typhidot Brochure - Reszon Diagnostics International

TYPHOID FEVER DIAGNOSTIC
Assay Procedure
Typhoid fever is an infectious disease caused by bacterium, Salmonella typhi.
It continues to be a major health problem especially in the Asia Pacific region,
the Indian subcontinent, Central Asia, Africa and South America. Definitive
clinical diagnosis of typhoid is unreliable because typhoid fever symptoms
mimic other diseases with fever that are common in this part of the world.
Clinical presentation very tremendously among patients and cover a wide
spectrume, hence the need for a good laboratory test.
Step 1
Add patient serum sample
to pre-dotted antigen strip.
Wash away unbound
patient antibody.
Step 2
Add HRP conjugated
secondry antibody. Wash
away unbond secondry
antibody.
Current methods to diagnose typhoid are by isolation of S. typhi from body
fluids and by detection of antibody using the Widal test. Both methods are
time-consuming with variable sensitivity. The culture method has a sensitivity
of 48-78% but among the pediatric age group the sensitivity could be lower.
Although the Widal test is widely used, its many limitations lead to difficulties in
its interpretation especially in areas of high endemicity.
Step 3
Add substatre and read
results.
We produce two types of diagnostics test kits for typhoid fever - Typhidot and
Typhidot Rapid test kits. Typhidot is using a more sensitive Enzyme
Immunoblot Assay; while Typhidot Rapid test kits are using a faster
Immunochromatography Assay.
Interpretation Of Result :
Typhidot
Typhidot is a revolutionary methos of diagnosis. Invented in Malaysia, it is the
first known qualitative antibody detection test design for rapid diagnosis of
typhoid fever. Using the Dot EIA technique, presence of IgM & IgG antibodies
against a specific antigen on the outer membrane of S. typhi are detected.
Typhidot has been evaluated by many endemic areas including Malaysia,
India, Indonesia, Pakistan and Philippines. A control clinical field trial among
febrile children admitted to a hospital in an endemic area showed that Typhidot
when compared to culture is sensitive (95%) and did not show any
cross-reactions.
RAPID, SENSITIVE AND SPECIFIC!!
Detects BOTH IgG and IgM - stages
of infection can be determined
simultaneously!!
Please refer to product insert for detailed instruction.
Features :
Platform
Format
Detection
: Enzyme Immunoassay
: Dot Blot Strip
: Human IgG and IgM against
specific S. typhi 50kD OMP
antigen
Specimen
: Serum
Specimen Volume : 5 µl (1 hour method)
20 µl (3 hours method)
Shelf Life
: 12 months
Assay Time
: 1 or 3 Hours
NO aaditional sample preparation
required!!
95% sensitivity and DID NOT
SHOW CROSS DETECTION
RTF-ED0101
Typhidot 1 hour
Salmonella typhi
28 T/ Kit
RTF-ED0102
Typhidot 3 hours
Salmonella typhi
28 T/ Kit
Typhidot Rapid Test Kits
Typhidot Rapid IgM
Assay Procedure
Step 1
Add serum/ plasma/
whole blood to square
well. For whole blood this
is followed by addition of
one drop buffer
Step 2
Up to area A, add 3 drops
buffer to oval well.
The test kits are designed for the qualitative detection and differentiation of
specific IgM or IgG/ IgM antibodies against specific S. typhi antigen.
Step 3
Pull clear plastic tab and
add one drop buffer to
square well
FAST!! Results within 15 minutes
Interpretation Of Result :
Results are EASY to INTERPRET
- test line appears means POSITIVE
RESULTS
MINIMAL sample volume is required
Features :
Platform
Format
Detection
Specimen
Specimen Volume
Shelf Life
Assay Time
Typhidot Rapid IgG & IgM (combo)
Assay Procedure
: Immunochromatography assay
: Cassette
: Human IgM or IgG/IgM against S.typhi OMP antigen
: Serum/ Plasma or Whole blood
: 30-35 µl
: 18 months
: 15 minutes
TYPHIDOT RAPID
IgM IgG
T
Step 1
Add serum/ plasma to
each sample well.
T
Step 2
Up to area C, add one
drop buffer to each well.
Read result within 15-20
minutes.
TYPHIDOT RAPID
IgM IgG
T
T
Interpretation Of Result :
Positive for
IgG & IgM
Positive for
IgG
IgM IgG
C
T
C
T
C
T
IgM IgG
C
T
C
T
Negative
C
T
Invalid
IgM IgG
IgM IgG
IgM IgG
C
T
Positive for
IgM
IgM IgG
C
T
C
T
C
T
C
T
C
T
Please refer to product insert for detailed instruction.
Ordering Information :
RTF-RD0101
Typhidot Rapid IgM
Salmonella typhi
25 T/ Kit
RTF-RD0202
Typhidot Rapid IgG IgM (combo)
Salmonella typhi
25 T/ Kit
Reszon Diagnostics International Sdn. Bhd.
917901-W
Revongen Corporation Center, No.12A, Jalan TP5, Taman Perindustrian UEP,
47600 Subang Jaya, Selangor Darul Ehsan, Malaysia.
T : +6 03 8025 1603
F : +6 03 8025 1637
E : [email protected]
W: www.reszonics.com