BOUGAINVILLEA (Bougainvillea Comm. ex Juss.)
Transcription
BOUGAINVILLEA (Bougainvillea Comm. ex Juss.)
Guidelines for conducting test for Distinctiveness, Uniformity and Stability on Bougainvillea (Bougainvillea Comm. ex Juss.) Protection of Plant Varieties and Farmers’ Rights Authority (PPV & FRA) Government of India 1 Contents 1. Subject 34 2. Planting Material Required for Testing 34 3. Procedure for Conducting of Test 35 4. Methods and Observations 35 5. Grouping of Varieties 36 6. Characteristics and Symbols 37 7. Table of Characteristics 38 8. Data Dictionary 44 9. Explanations for the Table of Characteristics 46 10. Literature 64 11. Working Group Details 64 12. Name of DUS Test Center 65 2 x 3 2m x 2m 7 % 4 1% a) b) दह ु रा c) d) श्वेत नील-लोहहत चटक लाल रं ग अन्य 5 7 * + 7 QL, QN PQ QL QN PQ (a) - (c) : MG MS VG VS 1. PQ VG अन्य VG 2. PQ 6 (a) अन्य 3. (*) QN (b) VG/ MS 4. QL (b) VG 5. QN (c) स्तम्भ : शूल विरल मध्यम सघन विशाखा, 3 5 7 स््लें डन् े स विक्सी िेररगेटा 6. (*) (+) QN (b) VG 7. (*) QN (b) VG 8. QN (c) VG / MS 9. (*) QN (c) MG/ MS 10. (*) QN (c) MG/ MS 11. (*) PQ (c) (+) VG 7 अन्य VG 12. (+) PQ (c) अन्य VG 13. (+) PQ (c) 14. PQ (a) अन्य नई ित्ती : िर्ण निीन ित्र अन्य शुभ्रा 1 2 3 4 5 VG ग्लैब्रा जाककररयाना मनोहर चन्र िेररगेटा डा. िी. िी. साने VG 15. (+) PQ (c) अन्य VG 16. (*) (+) PQ (c) अन्य VG 17. (+) 8 (c) अन्य VG 18. (+) PQ (c) अन्य VG 19. QN (c) अन्य VG 20. QL अन्य 21. QN (c) MG/MS 22. QL VG 23. (*) (+) QN (c) 24. MG/MS MG/MS 9 (*) (+) QN (c) 25. (+) QN MG/MS 26. (+) QN MG/MS 27. (+) QL VG 28. (+) QN VG/ MG 29. (+) QN VG अन्य 30. (+) QL 31. (*) (+) QL VG VG दह ु रा अन्य 32. QN MG/MS 33. QN MG/MS 34. (*) (+) PQ VG अन्य VG 35. (+) 10 अन्य PQ VG 36. (*) (+) PQ अन्य VG 37. (*) (+) PQ 38. QL अन्य चचरलग्न / चचरलग्न 39. (+) PQ (c) अिाती आशुिाती 1 महारा, जजन्ना बरात 2 अरुर्ा, डा. िी. िी. साने श्वेत चिम्मा क्रीम िर्ण लेडी ररचडण हरा रं ग ललए ड्रीम हुये िीताभ िीताभ सि ु र्ाण VG VG िािाण, एलीज़ाबेि एग्नस लाल नारं गी गुलाबी अन्य 40. QN िष्ु ि: रं ग लघु ग्लैडी हे िबनण मध्यम ड्िार्ण जेम चौडा रोज़ क्िीन, रॉयल डौ्लीने 41. QN िुष्ि प्रधान लमसेस बट सामान्य सुरेखा, डा. राि 42. (+) PQ (c) िुष्ि नललका: हरा स्िेता नारं गी अरुर्ा नील-लोहहत विशाखा लाल िालेकर बैगनी जल ु ु क्िीन रं ग अन्य 43. (+) PQ िुष्ि नललका: आकार ितला/दब ण ु ल ग्लैब्रा िराग-केसर (िुँु- VG VG VG मध्य में हल्का सा संकीर्णन आधार िर 44. QN VG िालेकर र्ूला हुआ अन्तस्िाणवित लमसेस बट बाहस्िाणवित इननड लंकास्टर, सुरेखा, 11 VG केसर) 45. (*) (+) PQ श्वेत VG स्िेता हरा सुिर्ाण िीला नारं गी नील-लोहहत गुलाबी लाल चटक लाल रं ग कारलमललयों र्ीस्टा बैगनी अन्य 46. (*) (+) PQ श्वेत शुभ्रा िीला सि ु र्ाण नारं गी अरुर्ा नील-लोहहत एलशया गुलाबी महात्मा गांधी लाल कारलमललयों र्ीस्टा चटक लाल रं ग ड्रीम बैगनी जल ु ु क्िीन अन्य 47. (*) (+) PQ VG श्वेत सोिा िीला इननड लंकास्टर नारं गी जाककररयाना नील-लोहहत गोिाल गुलाबी िॉलटोनी स्िेशल लाल फ्लेम चटक लाल रं ग डॉ॰ हरभजन लसंह बैगनी स््लें डन् े स VG अन्य 48. PQ श्वेत महरा व्हाइट िीला VG रोजविलीस डडलाइट नारं गी महरा नील-लोहहत लॉसबनास ब्युटी गुलाबी लाल चटक लाल रं ग बैगनी अन्य 49. PQ श्वेत महारा व्हाइट िीला 12 VG नारं गी अचणना नील-लोहहत महारा गुलाबी लॉसबनास ब्युटी िेररगेटा लाल चटक लाल रं ग बैगनी अन्य 50. (+) PQ श्वेत मेरी िामर स्िेशल िीला VG नारं गी नील-लोहहत गुलाबी चचत्रा लाल मेरी िामर स्िेशल चटक लाल रं ग बैगनी अन्य 51. (+) PQ श्वेत VG िीला नारं गी नील-लोहहत गुलाबी लाल चटक लाल रं ग बैगनी अन्य 52. (+) PQ श्वेत स्िेता िीला लेडी मेरी बररंग नारं गी लाउस बािन नील-लोहहत रानी गल ु ाबी कैसकेड लाल डॉ आर॰ आर॰ िाल चटक लाल रं ग िेनांग बैगनी ग्लैब्रा सेंडरे रयाना अन्य 8. 13 VG 14 (a), (b) (c) (a) (b) 15 (c) 16 17 18 19 20 21 22 23 24 25 26 27 28 29 श्वेत हरा िीला नारं गी नील-लोहहत गल ु ाबी लाल चटक लाल रं ग बैगनी अन्य 30 श्वेत हरा िीला नारं गी नील-लोहहत गुलाबी लाल चटक लाल रं ग बैगनी अन्य श्वेत हरा िीला नारं गी नील-लोहहत गुलाबी लाल चटक लाल रं ग बैगनी अन्य श्वेत हरा िीला नारं गी नील-लोहहत गल ु ाबी लाल चटक लाल रं ग बैगनी अन्य श्वेत हरा 31 िीला नारं गी नील-लोहहत गुलाबी लाल चटक लाल रं ग बैगनी अन्य श्वेत हरा िीला नारं गी नील-लोहहत गुलाबी लाल चटक लाल रं ग बैगनी अन्य 32 33 www.nbri.res.in 34 BOUGAINVILLEA (Bougainvillea Comm. ex Juss.) 1. Subject: These Test Guidelines will apply to all varieties, especially under the species Bougainvillea glabra Choisy, B. spectabilis Willd., B. peruviana Humboldt & Bonpl., B. x buttiana Holttum & Standl. etc., besides their hybrids and mutants. 2. Planting Material Required for Testing: 2.1 The Protection of Plant Varieties and Farmers’ Rights Authority (PPV&FRA) shall decide when, where and in what quantity and quality of plant material is required for testing of the new variety denomination for registration under Protection of Plant Varieties and Farmers’ Rights (PPV&FR) Act, 2001. Applicant submitting such plant material from country other than India shall make sure that all customs and quarantine requirements stipulated under relevant national legislations and regulations are fulfilled and complied with. 2.2 For all varieties, the minimum quantity of plant material, to be provided by the applicant, should be: 10 well rooted and established plants. Any specific requirement for the expression of characters pertaining to DUS test shall have to be specified by the applicant. 2.3 The plant material provided for DUS test should be healthy, vigorous and free from pests or diseases. 2.4 The plant material should NOT be treated with any chemicals and bio-physical agents. If it has been treated, full details of the treatment must be provided along with the application. 2.5 The applicant should submit plant material having genetic purity and uniformity besides data on method of propagation / multiplication for raising population. 3. Procedure for Conducting Test: 3.1 The minimum duration of the DUS test should be one complete growing cycle (minimum one year). However, in case of any inconsistency of some characters, the test is to be extended further for another complete growing cycles. 3.2 The test should be conducted at one test location with one replication planted at field and other in pots for better expression of the phenotypic characters. In case of non-expression of any diagnostic character at that specific location, the test is to be shifted to other suitable location for further visual examination. Otherwise, the applicant must provide details about the specific requirement of the character. 35 3.3 The field test should be carried out at a sunny location for expression of all test characteristics. The experimental site should be large enough to accommodate plants in rows having spacing 2x2 sqm. for proper vegetative growth and flowering. 3.4 Any kind of growth promoting hormones should NOT be used throughout the vegetative growth period and flowering. 3.5 In case of pot-varieties, testing shall be done only in pots of prescribed size. 3.6 In case of any discrepancy, additional test protocols for special purpose shall be recommended by the PPV&FR Authority. 4. Methods and Observations: 4.1 The morphological characteristics described in the Table of characteristics should be used for the DUS testing of varieties (Section-7). 4.2 Unless otherwise indicated, all observations of vegetative characteristics shall be made during the first flush of growth and bracts / flower arising in the central third of a flowering shoot. Colour of vegetative organs / bracts / flower shall be observed on plants exposed to maximum light. 4.3 For the assessment of colour characteristics, the latest Royal Horticultural Society (RHS) colour chart shall be used. Because daylight varies, colour determinations made against colour chart shall be made either in a suitable cabinet providing artificial daylight or in the middle of the day in a room without direct sunlight. The special distribution of illuminant for artificial daylight shall conform to the CIE Standard of Preferred daylight D 6500 and should fall within the tolerance set out in the British Standard 950, Part 1. These determinations shall be made with the plant part placed against a white background. The colour chart and version used should be specified in the variety description. 4.4 All observations on single character should be made on the three randomly selected plants. 4.5 For the assessment of uniformity, a population standard of 1% and an acceptance probability of at least 95% should be applied. In the case of a sample size of 10 plants, 1 off-type is allowed. 4.6 For the assessment of Distinctiveness and Stability, all observations on single plants should be made on 9 plants or parts taken from each of 9 plants and any other observations made on all plants in the test, disregarding any off-type plants. 36 4.7 In practice, it is not usual to perform tests of stability that produce results as certain as those of the testing of distinctness and uniformity. However, experience has demonstrated that, for many types of varieties, when a variety has been shown to be uniform, it can also be considered to be stable. Where appropriate, or in cases of doubt, stability may be tested, either by growing a further generation, or by testing a new plant stock to ensure that it exhibits the same characteristics as those shown by the previous material supplied. 5. Grouping of Varieties: 5.1 The candidate varieties for DUS testing shall be divided into groups to facilitate the assessment of Distinctiveness. Characteristics which are known from experience usually do not vary or to vary slightly with in a variety and which in their various states are fairly evenly distributed across all varieties in the collection are suitable for grouping purpose. 5.2 The following characteristics shall be used for grouping of Bougainvillea varieties: 6. 6.1 (a) (b) Plant Habit Leaf Blade : : (c) Inflorescence : (d) Young Bract : Erect, Semi-erect, Drooping, Spreading and Climbing Secondary colour (Characteristic 16)/ Variegated- Non variegated Types of bract (characteristic 31) Single / Multi / Double / Others Main colour of inner side (Calyx lobe/ Star open) (Characteristic 50) with the following groups: Group 1: White Group 2: Yellow Group 3: Orange Group 4: Magenta Group 5: Pink Group 6: Red Group 7: Mauve Group 8: Purple Group 9: Others Characteristics and Symbols To assess Distinctiveness, Uniformity and Stability, the characteristics and their states as given in the Table of characteristics (Section 7) shall be used. 37 6.2 Notes 0-9 (except bract / flower colour group) shall be used to describe the state of each character for the purpose of digital data processing. 6.3 Legend : (*) Characteristics that shall be observed during every growing season on all varieties and shall always be included in the description of the variety, except when the state of expression of any of these characters is rendered impossible by a preceding phenological characteristic or by the environmental conditions of the testing region. Under such exceptional situation, adequate explanation shall be provided. (+) See explanations on the Table of characteristics 6.4 Characteristics containing the following key in the fourth column of the Table of characteristics shall be examined as indicated below: QL : Qualitative characteristic QN : Quantitative characteristic PQ : Pseudo-qualitative characteristic 6.5 (a) - (c) : See section 8 for explanation 6.6 Type of assessment of characteristics indicated in column six of the Table of characteristics is as follows: 7. MG : Measurement by a single observation of a group of plants or parts of plants MS : Measurement of a number of individual plants or parts of plants. VG : Visual assessment by a single observation of a group of plants or parts of plants. VS : Visual assessment by observations of individual plants or parts of plants Table of Characteristics: S. No. Characteristics States Note Example Varieties Type of assess ment 1. PQ Upright Semi-upright Spreading Drooping Climbing Others 1 2 3 4 5 6 Pixie Variegata Chitra Aruna, Mahara Dr. H.B.Singh, Palekar Royal Daupline, Shubhra VG Plant: Growth Habit 38 Light green Medium green Reddish green Reddish Others Short Medium Long 1 2 3 4 5 3 5 7 Arjuna, Lilac Puff Shweta, Mary Palmer President Flame, Glady's Heburn VG Pixie Variegata Zinna Barat, Blondie Chitra, Spring Festival VG/ MS Stem: Thorns Absent Present 1 5 9 Mrs. Alice Sova, Cascade Chitra VG 5. QN (c) Stem: Density of Thorns Sparse Medium Dense 3 5 7 Vishakha Splendens Pixie variegata VG/ MG 6. (*) (+) QN (b) Thorn: Length Short Medium Long 1 2 3 Pixie Variegata, Pallavi Zinna Barat, Margery Lloyed Chitra, Refulgens VG 7. (*) QN (b) Thorn: Curvature Straight Slightly curved Fully curved 1 3 5 VG 8. QN (c) Thorn: Strength Weak Medium 1 2 Strong 3 Short Medium Long 3 5 7 Elizabeth Splendens, Dr.P.V.Sane,Aida Variegata Pradhan Profusion Sova, Jubilee Enid Lancaster, Begum Sikander Splendens, Pradhan Profusion Sova, Stanza, Laiteritia Parthasarthy, Philip Narrow Medium Broad 3 5 7 2. PQ (a) Young Shoot: Colour 3. (*) QN (b) Plant: Length of internodes 4. QL (b) 9. (*) QN (c) Leaf Blade: Length 10. (*) QN (c) Leaf Blade: Width VG MG/ MS Turley’s Special, Rosea Fuchsia Golden Glory, Feathery Fantasy Glady’s Heburn, Happiness Mary Palmer, Los BanosVariegata 39 MG/ MS 11. (*) PQ (c) (+) Leaf Blade: Shape 12. (+) PQ (c) Leaf Blade: Shape of Apex 13. (+) PQ (c) Leaf Blade: Shape of Base 14. PQ (a) Young Leaf: Colour 15. (+) PQ (c) Leaf Blade: Main Colour 16. (*) (+) PQ (c) Leaf Blade: Secondary colour 17. (+) (c) Leaf Blade: Distribution of Secondary Colour Lanceolate Medium ovate Broad ovate 1 2 3 Elliptic Circular Others Acuminate 4 5 6 1 Acute Obtuse Others Attenuate Acute Obtuse 2 3 4 1 2 3 Others Light green Medium green Reddish green Reddish Others 4 1 2 3 4 5 Yellowish white Yellow Yellowish green Light green Medium green Dark green Very dark green Grey green Others None White Yellowish white Yellow Light green Medium green Dark green Very dark green Grey green Others Absent Narrow-marginal Broad -marginal Around midrib 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 0 1 2 3 4 Zinna Barat, Mrs. Alice VG Shubhra, Thimma Mrs. But, Los Banos Variegata Palekar, Dream Archana, Camarillo Fiesta Liliac Perfection, Scarlet VG Queen Variegata Archana, Blondie Marietta Dream Marietta, Zinna Barat Thimma, Scarlet Queen Variegata VG Shubhra Glabra Zakiriana Manohar Chandra Variegata Dr. P.V. Sane Scarlet Queen Variegata Marietta, Aida Nirmal, Pallavi VG VG Surekha, Dream, Liliac Queen Dr.H.B.Singh Chitra Zulu Queen 40 Asia, Aida, Dream Silverline Arjuna , Abhimanyu Pixie Variegata Thimma Nirmal Pallavi Louise Wathen Royal Daupline VG Chitra, Mrs. Alice Silverline, Abimanyu Royal Daupline Scarlet Queen Variegata VG Speckled Irregular Others None White Yellowish white Yellow Light green Medium green Dark green Very dark green Grey green Others Absent or weak Medium Strong Others Glabrous Hairy Slightly Hairy Tomentose Others Sparse Medium Dense 5 6 7 1 2 3 4 5 6 7 8 9 0 1 2 3 4 1 2 3 4 5 3 5 7 Archna Parthasarthy ZakirianaVariegata Persistent Non Persistent Short Medium Long Petiole: Attitude Silverline Hawaiian white Pixie Variegata ZakirianaVariegata Aida Variegata Manohar Chandra Variegated Archana Scarlet Queen Variegata Los Banos Variegata Chitra, Mrs. Alice Aruna, Killie Campbell Crispa, Dr.R.R.Pal VG Crispa Splendens, Red Triangle Mrs. Alice Red Glory VG Chitra Zinna Barat Pixie Variegata MG/MS 1 2 1 2 3 Mrs. Alice Zinna Barat Glabra Magnifica Zinna Barat Mary Palmer VG Upward Horizontal Downwards 1 2 3 Fantasy Palekar Mahara MG/MS Inflorescence: Length Short Medium Long 3 5 7 MG/MS Inflorescence: Peduncle Length Short Medium Long 3 5 7 Aida Parthasarthy, Partha Shweta, Shubhra, Dream Pixie Variegata Zulu Queen Isabell Green Smith, 18. (+) PQ (c) Leaf Blade: Tertiary Colour 19. QN (c) Leaf Blade: Undulation of Margin 20. QL Leaf Blade: Texture 21. QN (c) Number of Leafs on Primary Branch Leaf Blade: Persistence Petiole: Length 22. QL 23. (*) (+) QN (c) 24. (*) (+) QN (c) 25. (+) QN 26. (+) QN 41 VG MG/MS MG/MS Palekar 27. (+) QL 28. (+) QN 29. (+) QN Inflorescence: Arrangement of Bract Clusters Inflorescence: Number of Bract Clusters Inflorescence: Density of Bract Clusters Terminal Axillary Axillary and Terminal Few Medium Many Sparse Medium Dense Others Absent Present 1 2 3 Single Multiple Double Others Short Medium Long Narrow Medium Broad Narrowly Ovate Medium Ovate Broadly Ovate Circular Others Reflexed Normal/ Straight Others 1 3 5 7 3 5 7 3 5 7 1 2 3 4 5 1 9 0 3 5 7 3 5 7 9 1 9 Aida, Mahara Parthasarthy, Pixie Shweta, Dr. Har Bhajan Singh Fantasy Chitra Shweta Mrs. Alice Tetra Mrs. Maclean Shubhra VG Cherry Blossom Shweta, Tetra Mrs. Mc Clean Shweta, President Mahara, Pallavi VG Pixie, Cherry Blossom Suvarna Mrs. Alice Feathery Fantasy Mahara Chitra IsabellGreensmith, Dream Archana, Dr. H.B. Singh Zulu queen Tetra Mrs. Mc Clean Mrs. Alice Feathery Fantasy MG/MS VG/ MG VG 30. (+) QL 31. (*) (+) QL 32. QN Inflorescence: Presence of Flowers Inflorescence: Type of Bract 33. QN Bract: Width 34. (*) (+) PQ Bract: Shape 35. (+) PQ Bract: Reflection 36. (*) (+) PQ Bract: Shape at Tip Acute Obtuse Others 1 2 3 Feathery Fantasy, Mahara Mary Palmer Special VG 37. (*) (+) PQ Bract: Shape at Base Acute Obtuse Cordate Others 1 2 3 4 Feathery Fantasy Zinna Barat, Cherry Blossom Aida, Lady Mary Baring VG Bract: Length 42 VG MG/MS VG VG 38. QL Bract: Persistence Persistent Non Persistent 1 2 Mahara, Zinna Barat Aruna, Dr. P.V. Sane VG 39. (+) PQ (c) Only varieties with inflorescence type of Bract: Single: Calyx Lobes/ Star: Colour of upper side White Creamy Greenish yellow Yellow Red Pink Orange Others 1 2 3 4 5 6 7 8 Thimma Lady Richard Dream Suverna Partha, Elizabeth Agnus VG 40. QN Star: Diameter Short Medium Broad 1 2 3 Glady Hepburn Dwarf Gem Rose Queen, Royal Daupline VG 41. QN Star: Prominent Non-Prominent 1 9 Mrs. Butt Enid Lancaster, Surekha, Dr. Rao VG 42. (+) PQ (c) Floral tube: Colour Green Orange Magenta Red Purple Others 1 2 3 4 5 6 Shweta Aruna Vishakha Palekar Zulu Queen VG 43. (+) PQ Floral tube: Shape Slenderwith little constriction in the middle Swollen at base 1 Glabra VG 2 Palekar Lady Mary Baring, Mrs. Butt Dr. Rao, Enid Lancaster, Surekha 44. QN Stamen Inserted Exerted 1 9 45. (*) (+) PQ Small young Bract: Main solour of outer side White Greenish-White Yellow Orange Magenta Pink Red Mauve Purple Others 1 2 3 4 5 6 7 8 9 0 Isabell Greensmith VG VG Shweta Suverna Partha Carmilio Fiesta Dr. H.B. Singh 43 46. (*) (+) PQ Young Bract: Main colour of inner side (Calyx lobe/ Star not open) White Yellow Orange Magenta Pink Red Mauve Purple Others 1 2 3 4 5 6 7 8 9 Shubhra Suverna Aruna Asia Mahatma Gandhi Carmillio Feista Dream Zulu Queen VG 47. (*) (+) PQ Young Bract: Main colour of inner side White Yellow Orange Magenta Pink Red Mauve Purple Others 1 2 3 4 5 6 7 8 9 Sova Enid Lancaster Zakiriana Gopal Poultoni Special Flame Dr. Harbhajan Singh Splendens VG 48. PQ Only varieties with inflorescence type of Bract: Double: Young outer Bract: Main colour of inner side White Yellow Orange Magenta Pink Red Mauve Purple Others 1 2 3 4 5 6 7 8 9 Mahara White VG Only varieties with inflorescence type of Bract: Double: Young inner Bract: Main colour of inner side White Yellow Orange Magenta Pink Red Mauve Purple Others 1 2 3 4 5 6 7 8 9 Mahara White Young Bract: Secondary colour of inner side (Calyx lobe/ Star open) White Yellow Orange Magenta Pink 1 2 3 4 5 Mary Palmer Special 49. PQ 50. (+) PQ Roseville’s Delight Mahara Los Banos Beauty VG Archana Mahara Los Banos Beauty Variegata Chitra Mary Palmer Special 44 VG Red Mauve Purple Others 6 7 8 9 51. (+) PQ Young Bract: Tertiary colour of inner side (Calyx lobe/ Star open) White Yellow 1 2 Orange Magenta Pink Red Mauve Purple Others 3 4 5 6 7 8 9 52. (+) PQ Bract: Main colour (Calyx lobe/ Star wilted / fading) White Yellow Orange Magenta Pink Red Mauve Purple Others 1 2 3 4 5 6 7 8 9 8. Shweta Lady Mary Baring Louise Wathen Ranee Cascade Dr. R.R. Pal Penang Glabra Sanderiana VG Data Dictionary: 1 2 Characteristics Absent Acuminate 3 4 Acute Attenuate 5 Axillary 6 7 8 Broad Circular Climbing 9 10 11 12 Cordate Curved Dark green Dense S. No. VG Description Not present. Tapering gradually to a sharp point Margins straight to convex forming a terminal angle 45-90. Elongate, tapering gradually to a narrow point, usually applied to base. Pertaining to or growing from the axil of plants; produced in the axil. Having ample extent from side to side or between limits. Having the form of a circle : round Plants which grow upward by means of tendrils, petioles, or adventitious roots. Heart-shaped (leaf base). Bent or formed into a curve. Colour type Having relatively high density. 45 13 14 15 Downwards Drooping Elliptic 16 17 18 19 20 Few Glabrous Grey green Hairy Horizontal 21 22 Irregular Lanceolate 23 24 Light green Long In a direction from higher to lower. Plants whose branches hang or incline downward. Broadening at or about the center and narrowing equally toward each end. Being more than one but indefinitely small in number. Lacking hairs, smooth surface, without pubescence of any kind. Colour type Covered with hair or hairlike projections. Parallel to, in the plane of, or operating in a plane parallel to the horizon or to a baseline. Dissimilar in shape and/ or size. Long and thin and broadest below the middle, tapering to a point like a lance; lance-shaped. Colour type A section or region of plant of longer length. 25 26 27 28 29 30 31 32 Many Marginal Medium Medium green Multiple Narrow Non Persistent Obtuse Being one of a large indefinite number; numerous. Pertaining to the border or edge. A section or region of stem of medium length. Colour type Having or involving more than one part, individual, etc. Not wide. Decomposed rapidly by environmental action. Margins straight to convex, forming a terminal angle more than 90. 33 34 Ovate Persistent 35 36 Reddish Reddish green The leaf is egg-shaped; the broadest part is below the middle. Leaves or flower petals that remain attached to the plant instead of dropping off. Colour type Colour type 37 Reflexed 38 39 Semi-upright Short 40 41 42 Single Sparse Speckled 43 44 45 Spreading Straight Strong Bent or recurved downward or backward; applied to bracts, petals, leaf-veins, etc. Plants which are partially errect. A section or region of plant of small length. Not accompanied by another or others; solitary. Not thickly grown or settled. Dotted or covered with speckles, especially flecked with small spots of contrasting colour. Plants whose branches grow in a more or less horizontal direction. Free from curves, bends, angles, or irregularities. Having strength or power greater than average or expected. 46 46 Terminal 47 48 Tomentose Upright 49 50 51 Upward Very dark green Weak 52 53 54 55 White Yellow Yellowish green Yellowish white Growing at the tip of a branch or stem, often applied to a bud, rosette, or flower. Covered with thickly matted, woolly hairs. Upright plants produce vertical branching which exceeds the length of their horizontal branching. In a direction from lower to higher. Colour type Lacking strength, not able to sustain or exert much weight, pressure, or strain. Colour type Colour type Colour type Colour type 9. Explanations for the Table of Characteristics: 9.1 Explanation Covering Several Characters The right stage of development for the assessment / evaluation of the characteristics is at the time of opening of one flower per bract in three inflorescences. In the case of double bracted varieties, observations should be made when one third of the bracts are fully developed and open. Characteristics indicated with (a), (b) and (c) in the first column of the Table of characteristics should be examined as indicated below: (a) Observations should be made on the upper third of the main shoot. (b) Observations should be made on the middle third of the main shoot. (c) Observations should be made on a developed leaf from the middle third of the main shoot. 9.2 Explanations for Individual Characteristics Characteristic - 1 Plant: Growth Habit Plant: Growth Habit Upright 47 Semi-upright Spreading Drooping Climbing Characteristics - 3 Plant: Length of Internodes 48 Plant: Length Short of Internodes Medium Long Characteristic - 4 Stem: Thorns Stem: Thorns Absent Present 49 Thorn: Curvature Characteristic - 6 Thorn: Length The natural length of thorn should be observed Characteristic - 7 Thorn: Curvature Straight Slightly Curved Fully Curved Characteristic - 11 Leaf Blade: Shape Leaf Blade: Shape Lanceolate 50 Medium Ovate Broad Ovate Elliptic Circular Characteristic - 12 Leaf Blade: Shape of Apex Leaf Blade: Shape of Apex Acuminate 51 Acute Obtuse Characteristic - 13 Leaf Blade: Shape of Base Leaf Blade: Attenuate Shape of Base Acute Obtuse Leaf Blade: Main Colour Characteristic - 15 Leaf Blade: Main Colour Yellowish white Yellow Yellowish green Light green Medium green Dark green Very dark green The main colour is the colour with the largest surface area. If the area of the colours is nearly half and half, the darker colour is the main colour. 52 Grey green Others Characteristic - 16 Leaf Blade: Secondary Colour None White Yellowish white Yellow Light green Medium green Leaf Blade: Secondary Colour The secondary colour is the colour with the second largest surface area. Dark green Very dark green Grey green Others Characteristic - 17 Leaf Blade: Distribution of Secondary Colour Leaf Blade: Distribution of Secondary Colour Absent Narrow Marginal Broad Marginal 53 Around Midrib Speckled Irregular Others Characteristic - 18 Leaf Blade: Tertiary Colour None White Leaf Blade: Tertiary Colour The tertiary colour is the colour with the third largest surface area. Yellowish white Yellow Light green Medium green Dark green Very dark green Grey green Others Characteristic - 19 Leaf Blade: Undulation of Margin 54 Leaf Blade: Absent or Weak Undulation of Margin Medium Strong Characteristic - 22 Leaf Blade: Persistence Persistent Non Persistent Leaf Blade: Persistence Tendency of leafs to fall together or one by one. Characteristic - 24 Petiole: Attitude Petiole: Attitude Upward 55 Horizontal Downwards Characteristic - 25 Inflorescence: Short Length Medium Long Inflorescence: Length The part of the shoot with coloured bracts is considered to be an inflorescence, irrespective of whether flowers are present or not. Characteristic - 26 Inflorescence: Peduncle Length Inflorescence: Short Peduncle Length Medium 56 Long Characteristic - 27 Inflorescence: Arrangement of Bract Clusters Inflorescence: Terminal Arrangement of Bract Clusters Axillary Axillary and Terminal Characteristic - 28 Inflorescence: Number of Bract Clusters 57 Inflorescence: Few Number of Bract Clusters Medium Many Characteristic - 29 Inflorescence: Density of Bract clusters Inflorescence: Sparse Density of Bract Clusters 58 Medium Dense Characteristic - 30 Inflorescence: Presence of Flowers Inflorescence: Absent Presence of Flowers Present Characteristic - 31 Inflorescence: Type of Bract Inflorescence: Single Type of Bract 59 Multiple Characteristic - 34 Bract: Shape Bract: Shape Narrowly Ovate Medium Ovate Broadly Ovate Circular Characteristic - 35 Bract: Reflection 60 Bract: Reflection Reflexed Normal/ Straight Characteristic - 36 Bract: Shape at Tip Bract: Shape at Tip Acute Obtuse Characteristic - 37 Bract: Shape at Base 61 Bract: Shape at Base Acute Obtuse Cordate Characteristic - 45 Small young White Bract: Main Greenish-White colour of Yellow outer side Small Young Bract: Main colour of outer side The main colour is the colour with the largest surface area. If the area of the colours is nearly half and half, the darker colour is the main colour. Orange Magenta Pink Red Mauve Purple Small young Bract Others Characteristic - 46 Young Bract: Main colour of inner side White Yellow Orange Young Bract: Main colour of inner side (Calyx lobe/ Star not open) The main colour is the colour with the largest surface area. If the area of the colours is nearly half and half, the darker colour is the main colour. 62 (Calyx lobe/ Star not open) Magenta Pink Red Mauve Purple Others Characteristic - 47 Young Bract: White Main colour Yellow of inner side Orange Young Bract – Calyx lobe/ Star not opened Young Bract: Main colour of inner side The main colour is the colour with the largest surface area. If the area of the colours is nearly half and half, the darker colour is the main colour Magenta Pink Red Mauve Purple Others Characteristic - 50 Young Bract: Secondary colour of inner side (Calyx lobe/ Star open) Young Bract: White Secondary Yellow colour of Orange inner side Magenta (Calyx lobe/ Pink Star open) Red The secondary colour is the colour with the second largest surface area. Mauve Purple Others Characteristic - 51 Young Bract – Calyx lobe/ Star opened Young Bract: Tertiary colour of inner side (Calyx lobe/ Star open) Young Bract: White Tertiary Yellow colour of Orange The tertiary colour is the colour with the third largest surface area. 63 inner side Magenta (Calyx lobe/ Pink Star open) Red Mauve Purple Others Characteristic - 52 Bract: Main colour of inner side (Calyx lobe/ Star wilted) Bract: Main colour of inner side (Calyx lobe/ Star wilted) White Yellow Orange Magenta Pink Red Mauve Mature Bract – Calyx lobe/ Star wilted Purple Others 64 10. 1. Literature: Pal, B. P. and Swarup, V. (1974) Bougainvilleas. Indian Agricultural Research institute, New Delhi. 2. Sharma, S.C. (1976) Bougainvilleas in India. Economic Botany Information Service, NBRI, Lucknow. 3. Choudhury, B. and Singh, B. (1981) The International Bougainvillea Check List. Indian Agricultural Research institute, New Delhi. 4. Singh, B., Panwar, R.S., Voleti, S.R., Sharma, V.K. and Thakur, S. (1999) The New International Bougainvillea Check List. Indian Agricultural Research institute, New Delhi. 5. Sharma, S.C. and Roy, R.K. (2000). Bougainvillea India’s Germplasm Collection. The National Plant Collections Directory 2000. National Council for the Conservation of Plants & Gardens (United Kingdom): 61-62 & 73. 6. Roy, R. K., Banerji, B.K. and Goel A.K. (2007). Bougainvillea – Germplasm collections at NBRI. Indian Bougainvillea Annual (20):10-13. 7. Sharma, S.C and Goel, A.K. (2006) Horto-taxonomy of Ornamentals: Case study of Bougainvillea. J Eco. Tax. Bot. 30(1): 71-78. 8. Roy, R.K. and Goel, A.K. (2008). Germplasm collection of Bougainvilleas at NBRI in Proc. National Conference on Bougainvillea, 2006, EBIS, NBRI, p 1-5. 11. Working Group Details: These test guidelines have been developed by a group of Scientists and technical staff members in consultation with other experts in the field and in commensurate with the international guidelines specially UPOV. 65 Scientific Team (4/2013): The scientific team consisted of Drs. R.K. Roy, Sr. Principal Scientist; Dr. S. Kumar, Principal Scientist; A.K. Goel, Chief Scientist and Dr. C.S. Nautiyal, Director, CSIR-NBRI as a Coordinator of the DUS test Centers at CSIR-NBRI, Lucknow. Supporting Staff: The team consisted of Project Assistants - Ms. Shilpi Singh, Dr. Ranjana, Sri Rameshwar Prasad and Dr. Satish Kumar, Tech. Asstt., Sri Girdhari Sharma, Sri Daya Shankar, Tech. Officer, Sri Shankar Verma, Senior Horticulture Officer and Shri N. C. Tewari, Sr. T.O. 12. Name of DUS test centre: Nodal DUS Centre CSIR-National Botanical Institute, Lucknow Other Test Centre Research Indian Agricultural Research Institute, New Delhi website:www.nbri.res.in 66
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