Manual Liquid Handling - John Morris Scientific
Transcription
Manual Liquid Handling - John Morris Scientific
Distributed by Manual Liquid Handling Catalog 2007/2008 Mexico Guadeloupe Martinique Venezuela Guyana Colombia Ecuador Peru Brazil Bolivia Chili Uruguay Japon South Korea China Taiwan Hong kong India Thailand Vietnam Malaysia Singapore Indonesia Australia Gilson Manual Liquid Handling USA Russia New Caledonia Argentina www.gilson.com I [email protected] I [email protected] I [email protected] Gilson, Inc. World Headquarters I 3000 Parmenter Street I P.O. Box 620027 I Middleton, WI 53562-0027 USA Tel: (1) 800-445-7661 or (1) 608-836-1551 I Fax: (1) 608-831-4451 Gilson S.A.S. I 19, avenue des Entrepreneurs I BP 145, F-95400 Villiers-le-Bel, France Tel: (33-1) 34 29 50 00 I Fax: (33-1) 34 29 50 20 LT800486E - © 2007 Gilson S.A.S. - All rights reserved - Printed in France, January 2007. Specifications subject to change without notice. Gilson, Inc. and Gilson S.A.S are ISO 9001 certified. oxygenemultimedia.fr New Zealand 2007/2008 Canada 14 1 Austria 10 2 Belgium 26 8 3 Bosnia and Herzegovina 19 7 18 Belarus 4 Bulgaria 15 31 21 23 5 Czech Republic 2 12 5 25 32 17 6 Croatia 9 30 1 13 24 27 6 3 29 7 Denmark 16 20 4 8 Estonia 22 28 11 Turkey 9 France Malta Cyprus 10 Finland Kuwait Tunis Israel 11 Greece Morocco Jordan Bahrain Pakistan 12 Germany Qatar Egypt 13 Hungary Saudia UAE Oman 14 Iceland Arabia 15 Ireland 16 Italy Senegal 17 Luxembourg 18 Lithuania Nigeria 19 Latvia 20 Macedonia Cameroon 21 Netherlands Kenya 22 Portugal 23 Poland Tanzania 24 Romania 25 Slovakia 26 Sweden 27 Slovenia Iles Maurice 28 Spain Reunion 29 Serbia & Montenegro 30 Switzerland South Africa 31 United Kingdom 32 Ukraine Microman® positive displacement pipette is now Hydrochloric acid Pipetman® Neo is an evolution of the classic available with a calibration certificate issued by the Gilson ISO 17025 accredited laboratory. The Gilson ISO 17025 accredited laboratory in France provides the undisputable guarantee and confidence that our Quality System and Standard Operating Procedures have been thoroughly evaluated and approved by a legitimate third party. Hydrofluoric acid Formic acid Nitric acid Phosphoric acid Propionic acid Gilson Safe Aspiration Station® is a stand-alone Sulfuric acid vacuum system that incorporates all the essential characteristics to aspirate quickly and safely all kinds of liquids and collect them in a safe container. Trifluoroacetic acid GmCLab is a compact and highly dependable ISO9001 Certified personal mini-centrifuge that quickly separates particles from the supernatant in a solid-liquid mixture or spin down liquids to the bottom of a tube. Gilson Reagent Reservoirs are ideal for dispensing reagent with multichannel pipettes. Two different types of reservoirs are available, 25 mL and 50 mL, standard or sterilized. Minipuls Evolution® laboratory peristaltic pump is the newest innovation from the makers of the Minipuls™3, offering unmatched flexibility and performance for liquid transfer applications. ISO17025 Accredited Benzyl alcohol Aniline Butanol / Butyl alcohol Chloroform Cyclohexane Diacetone alcohol Methylene chloride Diethylene glycol Dimethylformamide (DMF) Dimethylsulfoxide (DMSO) Dioxane (1,4) Ethanol Ether Formaldehyde Hexane Hydrogen peroxide Ammonium hydroxide Sodium hydroxide Sodium hypochlorite Methanol Methyl ethyl ketone Pentane Tetrahydrofuran (TH-) Urea 20% 50% 100% 10% 20% 37% 20% 40% 100% 10% 30% 65% 20% 85% 50% 100% 20% 50% 95% 20% 80% 100% 50% 20% 10% 40% 15% Cl PET = Polyethylene Terephthalate Nitril = Nitril EPDM = Ethylene Propylene LCP = Liquid Cristal Polymer PA = Polyamide PBT = Polybutylene Terephthalate Steel ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ PET N/A + + N/A ++ ++ ++ ++ + + + N/A ++ + N/A N/A ++ ++ + N/A N/A N/A ++ ++ ++ ++ + N/A ++ N/A ++ ++ ++ ++ N/A N/A ++ + N/A ++ ++ N/A ++ ++ Nitril ++ + + + ++ + + + + + + ++ ++ + ++ ++ ++ ++ ++ + ++ ++ + + ++ ++ + N/A EPDM ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ N/A N/A + + N/A N/A N/A N/A ++ ++ N/A ++ + N/A + ++ + ++ ++ ++ ++ ++ ++ ++ + + N/A PC = Polycarbonate PE = Polyethylene PVDF = Polyvinylidene fluoride TPX = Polymethylpentene POM = Polyoxymethylene PP = Polypropylene LCP N/A ++ ++ + ++ ++ + ++ ++ ++ + ++ ++ ++ + N/A N/A N/A N/A ++ ++ N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A ++ ++ N/A N/A ++ N/A N/A + N/A N/A ++ ++ ++ + ++ N/A + - PA ++ ++ ++ N/A ++ ++ + + + + ++ ++ + ++ N/A N/A ++ + ++ ++ ++ ++ ++ ++ N/A ++ ++ ++ ++ ++ N/A ++ ++ PBT N/A ++ ++ N/A N/A N/A N/A ++ + + + ++ + ++ ++ ++ ++ ++ + N/A N/A N/A N/A N/A ++ N/A N/A N/A ++ N/A ++ ++ ++ N/A ++ ++ + + + ++ ++ ++ N/A + ++ PC N/A ++ ++ + ++ ++ + ++ + ++ ++ + ++ ++ + ++ ++ + ++ + ++ ++ N/A N/A ++ ++ ++ ++ ++ ++ + ++ N/A PE ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ + ++ + ++ N/A + ++ ++ ++ ++ ++ + ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ PVDF N/A ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ + ++ ++ N/A + ++ ++ ++ ++ ++ N/A ++ ++ ++ ++ ++ + ++ ++ No chemical degradation + M edium resistance to chemical agents - L ow resistance to chemical agents N/A No data available TPX N/A + + N/A ++ ++ + ++ ++ ++ ++ ++ N/A ++ ++ ++ ++ ++ N/A N/A ++ ++ ++ N/A N/A N/A ++ N/A N/A + + N/A ++ ++ ++ N/A N/A ++ + ++ + ++ ++ ++ ++ ++ ++ + + + N/A POM ++ N/A + N/A ++ ++ + ++ + + + + + + + ++ + + ++ + ++ N/A ++ ++ ++ N/A ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ N/A ++ PP ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + ++ + ++ ++ N/A + ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ N/A + ++ Chemical compatibility table Pipetman P that addresses the growing susceptibility of pipette users to repetitive strain injuries. Product Acetamide Ethyl acetate Acetone Acetonitrile Acetic acid 47 MOTORIZED ELECTRONIC PIPETTES PIPETMAN Concept® (single and multichannel) Gilson Application notes The Gilson application notes collection is included in this new catalog edition for the first time, spanning the world of pipetting and providing pipette users with keys to a better understanding of even the most mundane aspects of pipetting. See the application notes insert Total commitment to our customers’ satisfaction In accordance with the ISO 9001:2000, our company’s organization is based on processes addressing customers’ needs. ADJUSTABLE AND FIXED VOLUME PIPETTES PIPETMAN® Ultra (single and multichannel) PIPETMAN® P PIPETMAN® Neo PIPETMAN® F PIPETTE KITS PIPETMAN® Ultra Starter Kit PIPETMAN® Micro-Volume Kit PIPETMAN® Starter Kit PIPETMAN® Large-Volume Kit ISO 17025 and Gilson pipettes 21 PIPETTE TIPS Diamond® Tips: Standard tips, Filter tips, Sterilized tips Packaging: TipackTM, Tower PackTM, SterilPackTM, EcoPackTM, EasyPackTM, Empty Racks POSITIVE DISPLACEMENT PIPETTES MICROMAN® and Capillary Pistons PIPETTE REPLACEMENT PARTS Diagnostics Medical Devices. High technical and metrological performances of our calibration laboratory Our independent pipette calibration laboratory is accredited by a national accreditation organization: - For its ability to calibrate pipettes in accordance with the ISO 8655. - For its capacity to reproduce reliable results. - For the reliability of its quality system. 18-19 MOTORIZED PIPETTING AIDS Gilson Pipetting Aid Our pipettes are designed, manufactured and calibrated in compliance All our pipettes comply with the IVD Directive (98/79/EC) for In Vitro 17 17 17 17 20 20 20 20 20 20 20 MANUAL AND ELECTRONIC REPEATER PIPETTES DISTRIMAN® and DistriTips® REPETMAN® and Repet-Tips Guarantee of our pipettes’ safety for In Vitro Diagnostics 8-11 12-13 14-15 16 PIPETTE ACCESSORIES CarrouselTM Gilson Reagent Reservoirs Name Tags SingleTM Pipette Holder TrioTM Pipette Stand ColorisTM Identification Clips Pipetman Concept® Rapid-Charge Stand High quality of conception and calibration of our products with the specifications and methods of the ISO 8655 standard. 4-7 ELECTRONIC MULTI-FUNCTION DILUTOR DISPENSERS 402 DILUTOR - DISPENSER BENCH-TOP SOLUTIONS MINIPULS Evolution® MINIPULSTM 3 Peristaltic Pump MINIPULS Applications MINIPULS Accessories Gilson Safe Aspiration Station GmCLab mini centrifuge LabStir magnetic stirrer GVLab vortex mixer 22-27 28-29 30 31 32-34 35 36-37 38 39 40 41 42 43 43 SERVICE Gilson Service network of trained distributors Gilson Preventive Maintenance On-Site Service The “Center of Excellence” Label Gilson training Pipetman® P Service Pack Do-it-yourself Performance Check: Weighing Kit 44 44 45 45 46 46 46 CHEMICAL COMPATIBILITY TABLE 47 PIPETMAN Concept ® Simply a Click Away From Perfection The new Pipetman Concept motorized electronic pipette is even more finely tuned to your needs, a combination of tangible quality with high usability and comfort. Conceived with simplicity and flexibility in mind, it features exclusive multifunction characteristics housed in a traditional Gilson quality design. Twelve models cover the full range of volumes from 0.5 μL to 10 mL with single and multichannel pipettes for higher productivity and greater comfort. Multifunction Concept Unique PC connections via USB interface Create your own pipetting modes with the PC based Utility Software that lets you create and store any number of modes on your PC and transfer them to your pipette (PC connection kit, Ref. F30755). Modes are easily created from pre-programmed tasks and can be exchanged on the Internet within a community of users. Five standard pipetting modes cover more than 95% of current application modes: Automatic pipetting, Automatic pipetting + Mixing, Manual, Repetitive, Reverse pipetting. Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Amazingly comfortable operation Real ergonomic design of handle and keyboard addresses the users’ concern for Repetitive Strain Injuries, backed up by extensive end-user trials. Adjustable tip-ejector button GLP Concept, make no compromise on quality • ISO 8655 and CE / IVD (98/79/EC) compliant. • Keypad disabled during pipetting. • The Utility Software allows locking of the system menu from your PC. • Pipette status always on screen. • Color coding to identify the model and corresponding Diamond® tip range. • Color-coded ID Tags to write your name or application. • User-defined stand-by screen text. For more details, see the application notes insert Calibration is easy and adaptable to your needs Adjustable hook Exclusive G-F.I.T. SystemTM. The exclusive Gilson Forceless Integrated Tip-ejection System provides instant reliable seals and low ejection forces for Large Volume (C5000, C10mL) and Multichannel models. G-F.I.T. System is compatible with most standard tips. Exceeds ISO 8655 Specifications. Pipetman Concept’s specifications are more stringent than those required to be ISO 8655 compliant. 4 3 Systematic error (µL) Adjustable tip-ejector stroke ISO 8655 PIPETMANConcept C300 specifications 2 1 0 -1 -2 -3 -4 0 50 100 150 200 250 300 Volume (µL) Learn more about Gilson’s exclusive G-F.I.T. System on page 9 Sy ste m 10% G - F. I . T. 50% 100% Powerful and easy user’s adjustment. Readjustment is performed at the 3 points of calibration recommended by the ISO 8655. A fourth calibration point enables you to maximize performance at a specific volume. PIPETMAN Concept ®® Sophisticated, Simply a Click Away Yet Simple Fromto Perfection Operate Manage your maintenance program Pipetman Concept’s internal software allows you to set the maintenance interval (weeks or cycles) and set the alarm that prompts you when it is time to service your pipette. PC based Utility Software gives you control of your maintenance program. You can store and organize maintenance reports, read data from, export data to your Pipetman Concept. Data compilation, simply essential for Q.A. Maintenance, calibration and system settings can be displayed on screen or on a PC when using the connection kit. Sophisticated, yet simple to operate Simple “One-step” command buttons. Just click on any of the Mode buttons to access one of five pipetting modes. Click and scroll with the thumbwheel to set the volume, to navigate or to select a menu option. Rapidly change the aspirate or dispense speeds (1 to 5) at any moment with the +/- buttons. Intuitive user interface. Sophisticated electronics driving a user-friendly display with selectable menus and straightforward navigation. Easy-change battery block. Optional rapid-charge stand charges Pipetman Concept pipette 80% in one hour, fully in two. Optional second battery block is kept charged and available for continuous operation. For more details, see the application notes insert Pipetman Concept always ready-to-use... Keeping track, Pipetman Concept does it for you All factory settings, calibration data and other useful information are stored in the internal memory and can be accessed at any time. On-screen indicator shows the number of times your pipette has been readjusted. ...on the rapid charge stand... using the power supply... using the additional Li-ion battery block Accessories AC-adapter Europe AC-adapter USA/Japan AC-adapter UK AC-adapter Australia Battery block Rapid Charge Stand PC connection Kit (including Utility Software) Pipetman Concept Utility Software Name Tags Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Ref. Number F30701 F30702 F30703 F30704 F3071165 F30750 F30755 F3075502 F3071107 Pipetman Concept Single-channel Models Currently not all models are available. Consult your nearest local distributor for availability. Model Diamond Tips* w/o AC-adapter Reference Number Volume (μL) Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Systematic Random error (μL) error (μL) Systematic Random error (μL) error (μL) C10 • D10 F31012 • DL10 0.5 - 10 μL 0.5 1 5 10 ± 0.040 ± 0.025 ± 0.060 ± 0.080 ≤ 0.013 ≤ 0.012 ≤ 0.020 ≤ 0.025 ± 0.120 ± 0.120 ± 0.120 ± 0.120 ≤ 0.080 ≤ 0.080 ≤ 0.080 ≤ 0.080 C100 • D200 F31013 5 - 100 μL 5 10 50 100 ± 0.35 ± 0.30 ± 0.38 ± 0.40 ≤ 0.10 ≤ 0.10 ≤ 0.12 ≤ 0.15 ± 0.8 ± 0.8 ± 0.8 ± 0.8 ≤ 0.30 ≤ 0.30 ≤ 0.30 ≤ 0.30 C300 • D300 F31014 20 - 300 μL 20 30 150 300 ± 0.80 ± 0.70 ± 0.90 ± 1.05 ≤ 0.16 ≤ 0.20 ≤ 0.23 ≤ 0.30 ± 4.00 ± 4.00 ± 4.00 ± 4.00 ≤ 1.50 ≤ 1.50 ≤ 1.50 ≤ 1.50 C1200 • D1200 F31015 100 - 1200 μL 100 120 600 1200 ± 2.5 ± 2.4 ± 3.6 ± 6.0 ≤ 0.4 ≤ 0.4 ≤ 0.8 ≤ 1.2 ± 16.0 ± 16.0 ± 16.0 ± 16.0 ≤ 6.0 ≤ 6.0 ≤ 6.0 ≤ 6.0 C5000 • D5000 F31016 500 - 5000 μL 500 2500 5000 ± 10 ± 15 ± 25 ≤ 2 ≤ 4 ≤ 7 ± 40 ± 40 ± 40 ≤ 15 ≤ 15 ≤ 15 C10ml • D10ml F31017 1 - 10 mL 1 mL 5 mL 10 mL ± 25 ± 30 ± 50 ≤ 4 ≤ 8 ≤ 12 ± 60 ± 60 ± 60 ≤ 30 ≤ 30 ≤ 30 * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available. (See pages 22-27) Pipetman Concept Multichannel Models Currently not all models are available. Consult your nearest local distributor for availability. Model Diamond Tips* w/o AC-adapter 8x10 • DL10 F31032 0.5 - 10 μL F31042 12x10 0.5 - 10 μL 1 5 10 ± 0.04 ± 0.08 ± 0.10 ≤ 0.02 ≤ 0.04 ≤ 0.06 ± 0.24 ± 0.24 ± 0.24 ≤ 0.16 ≤ 0.16 ≤ 0.16 8x100 • D200 F31033 5 - 100 μL F31043 12x100 5 - 100 μL 10 50 100 ± 0.25 ± 0.50 ± 0.80 ≤ 0.14 ≤ 0.20 ≤ 0.25 ± 1.60 ± 1.60 ± 1.60 ≤ 0.60 ≤ 0.60 ≤ 0.60 8x300 • D300 F31034 20 - 300μL F31044 12x300 20 - 300 μL 30 150 300 ± 1.00 ± 1.50 ± 2.40 ≤ 0.18 ≤ 0.38 ≤ 0.60 ± 8.00 ± 8.00 ± 8.00 ≤ 3.00 ≤ 3.00 ≤ 3.00 Reference Number Volume (μL) Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Systematic Random error (μL) error (μL) Systematic Random error (μL) error (μL) * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available. (See pages 22-27) PIPETMAN Ultra ® Advanced Pipetting at Your Fingertips Pipetman Ultra is a fully adjustable, air-displacement pipette with a patented multifunctional electronic display. Designed with robustness and precision in mind, it features exclusive enhancements and innovations that address the users concern for Repetitive Strain Injuries. Twelve models cover the full range of volumes from 0.2 µL to 10 mL with single and multichannel pipettes. For more details on ergonomics, see the application notes insert Probably the Most Comfortable Pipette Ever Whether you choose the 8 or 12 channel version, only two models of Pipetman Ultra Multichannel are needed to cover the entire volume range from 1 µL to 300 µL. Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Ultra Accuracy and Precision Improved comfort when fitting and ejecting tips Exclusive G-F.I.T. System™ provides instant reliable seals and low ejection forces for Pipetman Ultra and Pipetman Concept Large Volume (U5000; U10mL) and Multichannel models. G-F.I.T. System is compatible with most standard tips. Exclusive patented multifunctional electronic liquid crystal display with greater readability and zero risk of parallax errors. The force required to fit and eject Diamond tips using the G-F.I.T. System can be reduced by as much as half of that of regular tip-holders. Above 40 N, the ejection force remains at a steady level around 17 N (example for Pipetman Concept Multichannel 12x300). Reliable seal Stop & Guide Ejection force - Newton 35 Ultra comfort in daily use Ergonomic design fits naturally in your hand and requires less effort to use. Less force is needed to pipette for a smoother pipetting on a daily basis. 30 25 20 G-F.I.T. System Standard system 15 10 5 0 10 20 30 40 50 60 Fitting force (Newton) 2nd ridge: works as a guide and a stop Small contact areas Tip-holder Small contact areas = instant reliable seals + Quick volume setting Only ten turns are needed to change from the minimum to the nominal volume setting. 1st ridge: provides instant reliable seal Easy volume adjustment The thumbwheel’s rotating friction forces are reduced to a minimum. Adjustable patented tip-ejector works as a stop-block preventing over-insertion of the tip-holder into the tip (all models except U10mL). Natural movement Three-position tip-ejector button (patented) may be set for left- or right-handed users providing a natural accessibility. The lateral position of the button respects the hand’s anatomy, requiring less force to eject the tips. Maximum compatibility. The position of the tip-ejector can be adjusted to adapt most standard tips. Single-channel system low ejection forces Multichannel system PIPETMAN Ultra ® Higher Productivity Through Greater Comfort Ultra GLP and security features Lockable volume with on screen display for no risk of volume change during pipetting. Ultra easy maintenance Electronic adjustment is simple, only one tool is needed. Cycle counters in each Pipetman Ultra record piston strokes for quality control and ease of servicing. Channel numbers embossed on Multichannel heads reduce the chance of accidentally reversing sample order or cross-contaminating samples in plates. Color-coded thumbwheel and push button to identify the model (volume range) and the corresponding Diamond® Tips range. Two-part tip holder for easy maintenance and cleaning. Removable tip-ejector for easy access to the user-serviceable parts. Tip-ejector is made of PVDF to avoid corrosion. Color-coded stickers protected by a plastic window to personalize your Pipetman Ultra with your name or application. 10 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Pipetman Ultra Single-channel Models Model Diamond Tips* Reference Number Volume (μL) Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Systematic Random error (μL) error (μL) Systematic Random error (μL) error (μL) U2 • D10 F21021 • DL10 0.2 0.5 2 ± 0.024 ± 0.025 ± 0.030 ≤ 0.012 ≤ 0.012 ≤ 0.014 ± 0.08 ± 0.08 ± 0.08 ≤ 0.04 ≤ 0.04 ≤ 0.04 U10 • D10 F21022 • DL10 1 5 10 ± 0.025 ± 0.075 ± 0.100 ≤ 0.012 ≤ 0.030 ≤ 0.040 ± 0.12 ± 0.12 ± 0.12 ≤ 0.08 ≤ 0.08 ≤ 0.08 U20 • D200 F21023 2 5 10 20 ± 0.10 ± 0.10 ± 0.10 ± 0.20 ≤ 0.03 ≤ 0.04 ≤ 0.05 ≤ 0.06 ± 0.20 ± 0.20 ± 0.20 ± 0.20 ≤ 0.10 ≤ 0.10 ≤ 0.10 ≤ 0.10 U100 • D200 F21024 20 50 100 ± 0.35 ± 0.40 ± 0.80 ≤ 0.10 ≤ 0.12 ≤ 0.15 ± 0.80 ± 0.80 ± 0.80 ≤ 0.30 ≤ 0.30 ≤ 0.30 U200 • D200 F21025 20 50 100 200 ± 0.50 ± 0.50 ± 0.80 ± 1.60 ≤ 0.20 ≤ 0.20 ≤ 0.25 ≤ 0.30 ± 1.60 ± 1.60 ± 1.60 ± 1.60 ≤ 0.60 ≤ 0.60 ≤ 0.60 ≤ 0.60 U1000 • D1000 F21026 200 500 1000 ± 3 ± 4 ± 8 ≤ 0.6 ≤ 1 ≤ 1.5 ± 8 ± 8 ± 8 ≤3 ≤3 ≤3 U5000 • D5000 F21027 1000 2000 5000 ± 12 ± 12 ± 30 ≤ 3 ≤ 5 ≤ 8 ± 40 ± 40 ± 40 ≤ 15 ≤ 15 ≤ 15 U10ml • D10ml F21028 1mL 2mL 5mL 10mL ± 30 ± 30 ± 40 ± 60 ≤ 6 ≤ 6 ≤ 10 ≤ 16 ± 60 ± 60 ± 60 ± 60 ≤ 30 ≤ 30 ≤ 30 ≤ 30 * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available. (See pages 22-27). • P ipetman Ultra single-channel pipettes are available with a calibration certificate issued by an ISO 17025 accredited laboratory. (See pages 18-19). • A Pipetman Ultra Starter kit is available for an attractive price. (See page 17). Pipetman Ultra Multichannel Models Model Diamond Tips Reference Number Volume (μL) Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Systematic Random error (μL) error (μL) Systematic Random error (μL) error (μL) 8x20• DL10* F21040 12x20• D200 F21041 1 2 10 20 ± 0.10 ± 0.10 ± 0.20 ± 0.40 ≤ 0.08 ≤ 0.08 ≤ 0.10 ≤ 0.20 ± 0.40 ± 0.40 ± 0.40 ± 0.40 ≤ 0.20 ≤ 0.20 ≤ 0.20 ≤ 0.20 8x300 • D200 F21042 12x300• D300* F21043 20 30 150 300 ± 1.00 ± 1.00 ± 1.50 ± 3.00 ≤ 0.35 ≤ 0.35 ≤ 0.60 ≤ 1.00 ± 8.00 ± 8.00 ± 8.00 ± 8.00 ≤ 3.00 ≤ 3.00 ≤ 3.00 ≤ 3.00 * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available. (See pages 22-27) • Pipetman Ultra Multichannel pipettes are available with a calibration certificate issued by an ISO 17025 accredited laboratory. (See pages 18-19). 11 PIPETMAN P ® The Pipetting Standard Pipetman P is a fully adjustable, air-displacement pipette with the selected volume shown on a digital indicator (volumeter). Designed and manufactured to provide you with a range of robust, accurate and precise pipettes, eight single-channel models cover the full range of volumes from 0.2 µL to 10 mL. Easy Maintenance Routine cleaning is all that is required to keep Pipetman in top condition. No lubrication is required. Parts that may come in contact with liquid samples are easy to clean or replace. The tip-holder and tip-ejector are fully autoclavable. For more details on spare parts, see the application notes insert 12 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com The standard for Robustness P2 and P10 equipped with dual position tip-ejector (Ref. F2070903), allowing the use of Diamond D10 (short) and DL10 (long) tips : a plus for protection and precision when using microtubes. Built to last, constructed of stainless-steel and PVDF (a plastic with very high-resistance to chemical attack). The standard for Accuracy and Precision Unique “dry seal” technology avoids using grease to obtain adequate air-tightness, and guarantees long-term performances. P5000 and P10ml are safer and more accurate than glass pipettes. They are equipped with a polyethylene filter (Ref. F161241) that prolongs piston life expectancy and prevents contamination. Thick body (handle) in PVDF to minimize the hand-warming effect during pipetting. Rigorous quality control - each piston is checked using a microscope. Pipetman P Models Gilson Maximum Permissible Errors SO 8655 Maximum Permissible Errors Model Diamond Tips* Reference Number Volume (μL) Systematic error (μL) Random error (μL) Systematic error (μL) Random error (μL) P2 • D10 • DL10 F144801 ± 0.024 ± 0.025 ± 0.030 ≤ 0.012 ≤ 0.012 ≤ 0.014 ± 0.08 ± 0.08 ± 0.08 ≤ 0.04 ≤ 0.04 ≤ 0.04 P10 • D10 • DL10 0.2 0.5 2 F144802 ± 0.025 ± 0.075 ± 0.100 ≤ 0.012 ≤ 0.030 ≤ 0.040 ± 0.12 ± 0.12 ± 0.12 ≤ 0.08 ≤ 0.08 ≤ 0.08 P20 • D200 1 5 10 F123600 ± 0.10 ± 0.10 ± 0.10 ± 0.20 ≤ 0.03 ≤ 0.04 ≤ 0.05 ≤ 0.06 ± 0.20 ± 0.20 ± 0.20 ± 0.20 ≤ 0.10 ≤ 0.10 ≤ 0.10 ≤ 0.10 P100 • D200 2 5 10 20 F123615 ± 0.35 ± 0.40 ± 0.80 ≤ 0.10 ≤ 0.12 ≤ 0.15 ± 0.80 ± 0.80 ± 0.80 ≤ 0.30 ≤ 0.30 ≤ 0.30 P200 • D200 20 50 100 F123601 ± 0.50 ± 0.80 ± 1.60 ≤ 0.20 ≤ 0.25 ≤ 0.30 ± 1.60 ± 1.60 ± 1.60 ≤ 0.60 ≤ 0.60 ≤ 0.60 P1000 • D1000 50 100 200 F123602 ±3 ±4 ±8 ≤ 0.6 ≤1 ≤ 1.5 ±8 ±8 ±8 ≤3 ≤3 ≤3 P5000 • D5000 200 500 1000 F123603 ± 12 ± 12 ± 30 ≤3 ≤5 ≤8 ± 40 ± 40 ± 40 ≤ 15 ≤ 15 ≤ 15 P10ml • D10ml 1000 2000 5000 F161201 1mL 2mL 5mL 10mL ± 30 ± 30 ± 40 ± 60 ≤6 ≤6 ≤ 10 ≤ 16 ± 60 ± 60 ± 60 ± 60 ≤ 30 ≤ 30 ≤ 30 ≤ 30 * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available. (See pages 22-27). • Pipetman P pipettes are available with a calibration certificate issued by an ISO 17025 accredited laboratory. (See pages 18-19). • A large range of Pipetman P kits is available for an attractive price. (See page 17). • A special service offer is available with Pipetman P pipettes. (See page 46). 13 PIPETMAN Neo ® The Pipetting Standard with Lighter Spring Forces Pipetman Neo is an evolution of the classic Pipetman P that addresses the growing susceptibility of pipette users to repetitive strain injuries. Pipetman Neo exhibits a distinct enhancement in comfort a wide useful volume-range with the same legendary robustness, accuracy and precision as Pipetman P. Six single-channel models cover a range of volumes from 0.2µL to 1000µL. Wide Useful Volume Range Wide useful volume range: 10-100% of the nominal volume. Specifications provided at 10%, 50% and 100% of the nominal volume, as recommended by the ISO8655 standard. As for all Gilson pipettes, Pipetman Neo specifications are more stringent than those required for ISO8655 compliance. 14 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Color-coded push-buttons Up to 50% reduction of pipetting forces Color-coded push-buttons facilitate identification of the different models and corresponding quality Diamond tips. Newly engineered springs are wound to provide a significant reduction of pipetting and purge forces. Up to 50% reduction of pipetting and purge forces on certain models. Pipetman Neo was elaborated on the Pipetman P, maintaining its legendary robustness, accuracy and precision. 45 Dispensing Plunger force (N) 40 Purge 35 P200 30 New P200N Helps differentiate Pipetman Neo pipettes from the classic Pipetman P models. 25 20 15 10 5 0 0 5 10 15 20 25 Piston stroke (mm) For more details on ergonomics, see the application notes insert Pipetman Neo Models Model Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Diamond Tips* Reference Number Volume (μL) Systematic error (μL) Random error (μL) Systematic error (μL) Random error (μL) P2N • D10 • DL10 F144561 ± 0.024 ± 0.025 ± 0.030 ≤ 0.012 ≤ 0.012 ≤ 0.014 ± 0.08 ± 0.08 ± 0.08 ≤ 0.04 ≤ 0.04 ≤ 0.04 P10N • D10 • DL10 0.2 0.5 2 F144562 ± 0.025 ± 0.075 ± 0.100 ≤ 0.012 ≤ 0.030 ≤ 0.040 ± 0.12 ± 0.12 ± 0.12 ≤ 0.08 ≤ 0.08 ≤ 0.08 P20N • D200 1 5 10 F144563 ± 0.10 ± 0.10 ± 0.20 ≤ 0.03 ≤ 0.05 ≤ 0.06 ± 0.20 ± 0.20 ± 0.20 ≤ 0.10 ≤ 0.10 ≤ 0.10 P100N • D200 2 10 20 F144564 ± 0.35 ± 0.40 ± 0.80 ≤ 0.10 ≤ 0.12 ≤ 0.15 ± 0.80 ± 0.80 ± 0.80 ≤ 0.30 ≤ 0.30 ≤ 0.30 P200N • D200 10 50 100 F144565 ± 0.50 ± 0.80 ± 1.60 ≤ 0.20 ≤ 0.25 ≤ 0.30 ± 1.60 ± 1.60 ± 1.60 ≤ 0.60 ≤ 0.60 ≤ 0.60 P1000N • D1000 20 100 200 F144566 100 500 1000 ±3 ±4 ±8 ≤ 0.6 ≤1 ≤ 1.5 ±8 ±8 ±8 ≤3 ≤3 ≤3 * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available. (See pages 22-27). 15 PIPETMAN FConcept ® ® Sophisticated, Right Pipette! Right Yet Simple Volume! to Operate Right Price! Right volume Pipetman F is a fixed-volume, air-displacement pipette. Fourteen robust models cover a large volume range from 2 µL to 5000 µL, with legendary Pipetman accuracy and precision. Can be readjusted by users in the laboratory, for dense or viscous fluids (once reset the new volume cannot be changed accidentally). Right pipette Right price GLP compliance - no risk of volume selection errors. Using a dedicated fixed-volume Gilson pipette for a specific test assures reliable and consistent results. An economical choice for clinical diagnostics, quality control, and any routine testing. Pipetman F Models Model F2 F5 F10 F20 F25 F50 F100 F200 F250 F300 F400 F500 F1000 F5000 Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Systematic Random error (μL) error (μL) Systematic Random error (μL) error (μL) Diamond Tips* Reference Number Volume (μL) • D200 • D200 • D200 • D200 • D200 • D200 • D200 • D200 • D1000 • D1000 • D1000 • D1000 • D1000 • D5000 F123770 2 ± 0.08 ≤ 0.03 ± 0.08 ≤ 0.04 F123771 5 ± 0.10 ≤ 0.04 ± 0.125 ≤ 0.075 F123772 10 ± 0.10 ≤ 0.05 ± 0.12 ≤ 0.08 F123604 20 ± 0.20 ≤ 0.06 ± 0.20 ≤ 0.10 F123775 25 ± 0.25 ≤ 0.07 ± 0.50 ≤ 0.20 F123778 50 ± 0.40 ≤ 0.15 ± 0.50 ≤ 0.20 F123784 100 ± 0.80 ≤ 0.25 ± 0.80 ≤ 0.30 F123605 200 ± 1.60 ≤ 0.30 ± 1.60 ≤ 0.60 F123787 250 ± 3.00 ≤ 0.75 ± 4.00 ≤ 1.50 F123788 300 ± 3.50 ≤ 0.75 ± 4.00 ≤ 1.50 F123789 400 ± 3.60 ≤ 0.80 ± 4.00 ≤ 1.50 F123790 500 ± 4.00 ≤ 1.00 ± 4.00 ≤ 1.50 F123606 1000 ± 8.00 ≤ 1.30 ± 8.00 ≤ 3.00 F123607 5000 ± 30.00 ≤ 8.00 ± 40.00 ≤ 15.00 Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available. (See pages 22-27) • Pipetman F pipettes are available with a calibration certificate issued by an ISO 17025 accredited Laboratory. (See pages 18-19). 16 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com & Pipetman Ultra ranges for all of your pipetting needs. All in One Each kit contains two or three classic Pipetman P or Pipetman Ultra models plus samples of certified-quality Diamond Tips and useful accessories, such as Single Pipette Holders (except Large Volume Kit). Economical offer Save money when compared to pipettes and accessories bought separately. Pipetman Kits contents Model Reference Number Pipetman models Diamond Tips (racks of 96 tips) Accessories Pipetman Ultra Starter Kit F267630 U20 U200 U1000 D200 and D1000 • 3 Single Pipette Holders • 3 x 5 colored Name Tags • 1 Calibration key • 3 Lubricant tubes Pipetman Micro-Volume Kit Pipetman Starter Kit F167500 F167300 P2 P10 P100 P20 P200 P1000 Sterile FilterTips DFL10ST and DF100ST • 3 Single Pipette Holders D200 and D1000 • 3 Single Pipette Holders • 10 Coloris identification clips • 1 Gilson Guide to Pipetting • 10 Coloris identification clips • 1 Gilson Guide to Pipetting Pipetman Large-Volume Kit F167400 P5000 P10ml 5 x D5000 tips 2 x D10ml tips (no racks) • 2 x 10 protection filters for P5000 and P10ml ® Our Pipette Kits now include the Pipetman P PIPETMAN Pipette kits All You Need to Get Started, in a Single Box! 17 ISO 17025 and Gilson Pipettes Gilson Pipette with a Calibration Certificate Issued by ISO 17025 Accredited Laboratory Around the world, accreditation of pipette testing and calibration laboratories is based on the ISO 17025 international standard. ISO 17025 accreditation enables test data to be internationally validated and accepted. This system of international mutual recognition allows test data to be equivalent throughout the world because they are linked to the same metrological chain of international standard weight. Hence, the measure of the uncertainties derived from equipment, technicians, pipettes and tips which indicates the error range attributable to the measured quantities allows the accredited laboratory to attest to the pipette’s reliability and ensures that it is within • Gilson ISO 17025 accredited laboratory in France provides the undisputable guarantee and confidence that our Quality System and Standard Operating Procedures have been thoroughly evaluated and approved by a legitimate third party. • Gilson offers accredited calibration for new and serviced pipettes in full compliance with the ISO 17025 & ISO 8655 requirements. A certificate of calibration, approved by the French organization of accreditation (COFRAC), is delivered with each pipette. The certificate is recognized by international organizations of accreditation. In an on-going effort to address the growing demands for enhanced traceability and affordable ISO 17025 solutions, Gilson has been granted accreditation by the COFRAC for a special Gilson 3 vol. x 4 measurements method shortly after being accredited for the standardized ISO 8655 3 vol. x 10 measurements method. This enables us to offer ISO 17025 calibration at an attractive price. acceptable limits after calibration. • The standardized ISO 8655 method (3 vol. X 10 measurements). • The special Gilson method (3 vol. X 4 measurements). Gilson successfully demonstrated the absolute equivalence between the results obtained with both methods. Gilson’s pipette Calibration Laboratory in France This certificate includes the uncertainty budget defining the uncertainties for the involved measurements. Mass accreditation for pipette calibration n° 2-1630 The calibration certificate with the uncertainty measurement is approved by the French Organization of Accreditation (COFRAC). 18 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com New Pipettes with a Calibration Certificate Issued by the Gilson ISO 17025 Accredited Laboratory Serviced Pipettes with a Calibration Certificate Issued by ISO 17025 Accredited Laboratory Some Gilson service centers in the world are accredited (ISO 17025) for pipette calibration when using the gravimetric method (ISO 8655). Model Reference Reference ISO 8655 Method Gilson Method 3x10 measurements 3x4 measurements Pipetman F2 F123770C F123770CG Pipetman F5 F123771C F123771CG Pipetman F10 F123772C F123772CG Pipetman F20 F123774C F123774CG Pipetman F25 F123775C F123775CG Pipetman F50 F123778C F123778CG Pipetman F100 F123784C F123784CG Pipetman F200 F123605C F123605CG F123787CG If your nearest service center is accredited, you can ask for a certificate of calibration including a certificate of conformity for your pipette. This certificate is recognized throughout the world and linked to the International System of Units. If your local Gilson service center is not yet accredited, Gilson’s Accredited Laboratory of calibration based in France can provide you with two service offers : Pipetman F250 F123787C Pipetman F300 F123788C F123788CG Pipetman F400 F123789C F123789CG 1 - Accredited Cofrac calibration without repair ISO 8655 3 X 10 MEASUREMENTS METHOD (no tip change) Pipetman F500 F123790C F123790CG Pipetman F1000 F123606C F123606CG Reference Description Fixed volume Pipettes Pipetman F5000 F123607C F123607CG PCI 001 Pipetman P2 F144801C F144801CG PCI 002 Adjustable volume Pipettes PCI 003 8 channel Pipettes PCI 004 12 channel Pipettes Pipetman P10 F144802C F144802CG Pipetman P20 F123600C F123600CG Pipetman P100 F123615C F123615CG Pipetman P200 F123601C F123601CG Pipetman P1000 F123602C F123602CG Pipetman P5000 F123603C F123603CG Pipetman P10ml F161201C F161201CG Pipetman U2 F21021C F21021CG Pipetman U10 F21022C F21022CG Pipetman U20 F21023C F21023CG Pipetman U100 F21024C F21024CG Pipetman U200 F21025C F21025CG Pipetman U1000 F21026C F21026CG Pipetman U5000 F21027C F21027CG Pipetman U10ml F21028C F21028CG Pipetman Ultra 8x20 F21040C F21040CG Pipetman Ultra 12x20 F21041C F21041CG Reference Description Pipetman Ultra 8x300 F21042C F21042CG PGCM 001 Fixed volume Pipettes GILSON 3 X 4 MEASUREMENTS METHOD (no tip change) Reference Description PGCI 001 Fixed volume Pipettes PGCI 002 Adjustable volume Pipettes PGCI 003 8 channel Pipettes PGCI 004 12 channel Pipettes 2 - Repair* and accredited “Cofrac calibration” ISO 8655 3 X 10 MEASUREMENTS METHOD (no tip change) Reference Description PCM 001 Fixed volume Pipettes PCM 002 Adjustable volume Pipettes PCM 003 8 channel Pipettes PCM 004 12 channel Pipettes GILSON 3 X 4 MEASUREMENTS METHOD (no tip change) Pipetman Ultra 12x300 F21043C F21043CG PGCM 002 Adjustable volume Pipettes Microman M10 F148501C F148501CG PGCM 003 8 channel Pipettes Microman M25 F148502C F148502CG PGCM 004 12 channel Pipettes Microman M50 F148503C F148503CG * Spare parts not included Microman M100 F148504C F148504CG Microman M250 F148505C F148505CG Microman M1000 F148506C F148506CG This offer (2) includes the systematic replacement of the seal and o-ring. If Gilson determines that some parts need to be changed, Gilson will ask for your approval of a revised estimate. 19 Pipette Accessories Quality Accessories for Quality Pipettes Gilson Reagent Reservoirs Gilson accessories are of the same high quality as Gilson pipettes. Gilson considers accessories as a part of the pipetting system. All pipettes should be stored vertically to avoid the possibility of liquid running back into, and contaminating or corroding the interior of the pipette. Carrousel™ Pipette Stand The carrousel holds up to seven Gilson pipettes, all models with tips fitted (except Pipetman Concept®). Its rotating head provides easy access to the desired unit. Carrousel is robust, stable and space saving, making your work bench look neat. Its heavy base prevents toppling over. Disposable reagent reservoirs are ideal for dispensing reagent with multichannel pipettes (constructed of white polystyrene), two different types are available, 25 mL and 50 mL, standard or sterilized (via Gamma irradiation and individually wrapped). The Gilson Reagent Reservoirs meet stringent criteria of reliability and quality. Name Tags Personalize your Pipetman Concept and Pipetman Ultra by writing your name and application on one of the colored stickers (protected by the plastic window). Single™ Pipette Holder Single is designed to conveniently hold all Gilson Pipettes except Pipetman Concept where space is limited (fume hoods, sterile hoods, etc.). Stuck to any smooth, dry surface or fastened with screws, it keeps your pipette within reach. Resistant to chemicals and UV light. Trio™ Pipette Stand The new Trio stand features enhanced quality and reliability. Its angled, transparent design provides a convenient and secure way to store your Gilson pipettes (any models except Pipetman Concept) with exceptional stability. Its transparent body allows you to see the objects placed behind. Pipetman Concept Rapid-Charge Stand This robust stand allows to store the Pipetman Concept in an upright position while charging 80% in one hour, fully in two. A second battery block can also be kept charged and available for continuous operation. 20 Coloris™ Identification Clips GLP-friendly, these brightly colored clips are used to identify or personalize your Pipetman pipettes. Just write your name or application to avoid mix-ups. Made of polyacetal, Coloris clips accept permanent ink and just snap into place. Description Ref. Number Carrousel for 7 pipettes F161401 Single pipette holder F161406 Trio, stand for 3 pipettes F161405 Rapid charge stand, PIPETMAN CONCEPT F30750 Reagent Reservoir 25ml, 10 packs of 10 reservoirs F267660 Reagent Reservoir 50ml, 10 packs of 10 reservoirs F267670 Sterilized Reagent Reservoir 25ml, 50 individually wrapped F267661 Sterilized Reagent Reservoir 50ml, 50 individually wrapped F267671 Set of 5 name tags, PIPETMAN ULTRA F2070901 Set of 5 name tags, PIPETMAN CONCEPT F3071107 10 Coloris clips, mixed colours, PIPETMAN F161301 10 Coloris clips, red, PIPETMAN F161302 10 Coloris clips, yellow, PIPETMAN F161303 10 Coloris clips, green, PIPETMAN F161304 10 Coloris clips, blue, PIPETMAN F161305 10 Coloris clips, white, PIPETMAN F161306 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Gilson Pipetting Aid Rapid and Controlled Pipetting with Precision and Safety Comfortable Ergonomic design for increased user comfort. Quiet motorized operation. Precise and smooth finger tip control of variable speed range. Safe to use Safety valve and filter prevent contamination. “Wings” to enable you to rest it on the bench with no risk of contamination. All parts in contact with liquid are autoclavable. CE certified. High performance Powerful battery, continuous work for 8 hours (Environmentally friendly NiMH). Works with all standard glass and plastic pipettes. Fills 25 mL pipette in 4 seconds. Selectable operation modes according to volume and viscosity. Tip adapter for D5000 and D10ml with filter. Ergonomic solution to handle liquid volumes from 1 mL to 10 mL. Gilson Pipetting Aid* Description Charging and storage stand. The Gilson Pipetting Aid is always fully charged and ready to use. Reference Number Gilson Pipetting Aid (220V/50Hz) with European standard plug F161250 Gilson Pipetting Aid (220V/50Hz) without power supply F161240 Charging stand F161288 Adapter for D5000 and D10ml Diamond Tips F161287 Pipette holder F161256 Nose-piece F161249 Nose-piece hanger F161253 PTFE autoclavable filter 0.2 µm (5 units) F161245 PTFE autoclavable filter 0.45 µm (5 units) F161246 * Currently not available in the US - If you want to order a Gilson Pipetting Aid with a US, UK, or other countries’ power supply, please contact your local Gilson distributor. 21 DIAMOND Tips ® High Quality Tips for High Quality Pipettes Genuine Gilson Diamond® tip Fully traceable Pure polypropylene Pipetman® specifications are valid from the mold to ensures no contamination only with the use of Diamond tips your laboratory bench and complete dispensing Pure Performance Tips Perfect airtight seal Best match between pipette and tip ensures no leak reduces the strain to fit & eject tips A large choice of packaging Your needs, budget and tip consumption vary according to your activity. Gilson offers one of the largest ranges of packaging from large quantity bulk tips to an innovative refill system to satisfy virtually all requirements. Bags, the Economical Solution Available in convenient small bags or in economical bulk quantities, bags are available in 2 formats: EcoPack = 10 bags of 1,000 tips EasyPack = 5 bags of 200 tips All bulk bags are sealed with a sealing strip at the top. This seal can be easily torn off the bag to access the resealable zipper which keeps the tips secure within the bag. 22 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com “ Your protocols and experiments require more than just generic pipette tips. Accuracy and precision of your pipetting system is intimately linked to the performance delivered by the pipette-tip couple“. Tips are essential components of your pipetting system. By not using recommended tips, you put your experiments at risk. A filter tip No contamination you can trust Sterilized Diamond® tips are free of biological Fully autoclavable contaminants with a SAL of 10-6 Volume graduations make it easy to visually Fine point check the volume pipetted Diamond® tip geometry delivers maximum accuracy TowerPack™, the Innovative Refill System Tipack™ Engineered to be functional, convenient and robust. • Discover a quick and easy rack refill at an affordable price. Use 60% less space on your bench and 70% less plastic. • Tipacks are securely sealed with a colorcoded sticker. It includes the batch number and the tip’s maximum volume. • Robust, designed to withstand autoclaving more than 100 times. The patented Rocky Rack™ system available on TowerPacks™ and Tipacks™ makes it extremely easy to fit tips on a multichannel pipette. Tips will not fall off nor will they have to be manually positioned. Place the tower on the Press down firmly reload box with your hand Tip-Pipette color-coded labels and stickers The rack snaps into place 23 DIAMOND Tips ® High Quality Tips for High Quality Pipettes Superior Accuracy and Precision guaranteed Best Match between Pipette and Tips Reduces the Strain to Fit & Eject Tips “The maximum permissible errors always apply to the total system of piston pipette and tip. When using pipette tips not delivered by the pipette supplier, the supplier’s declaration or certificate of conformity does not apply” (ISO 8655-2)*. Pure Polypropylene Ensures no Contamination and Complete Dispensing Diamond Tip Geometry Delivers Maximum Accuracy Diamond tips guarantee that Gilson pipettes meet our specified tolerances and deliver consistent results over time. Evaluation of the influence of different tip brands of comparable quality (A, B,....I) on the accuracy and precision of a Pipetman® P10 at 1µl. Tip brands: DL-10 A B C D E F G H I Gilson specifications 0,018 Precision (µl) 0,016 0,014 0,012 0,01 0,008 Diamond tips are made from the highest grade virgin polypropylene to prevent contamination of your experiments. This polypropylene has high hydrophobic properties to ensure the lowest possible retention no matter what liquid is pipetted. Prevention of Contamination Often overlooked as a source of sample contamination, the pipette tip comes into direct contact with the sample and may contaminate the solution. Designed to meet the most stringent demands in various fields and applications such as PCR, molecular biology or cell technology. A Filter Tip You Can Trust 0,006 0,004 0,002 0 -0,125 ith Diamond tips no need to jam them on “extra W tight” – use of excessive force that causes muscular stress and fatigue to the arm and shoulder is greatly reduced. As a result, thumb force to eject tips is minimal. -0,1 -0,075 -0,05 -0,025 0 0,025 Accuracy (µl) Results obtained in the Gilson ISO 17025 Cofrac accredited laboratory from 10 measurements of 1μL of water at 21°C using a Pipetman P10. Adding Comfort while Fitting and Ejecting Tips Loose tip fitting is a major cause of bad performance. Specifically designed for the Gilson pipettes, the diameter of the tip-collar and tip-holder of the Gilson Diamond tips are calculated to fit perfectly. Equipped with a high density polypropylene filter, Diamond tips contain no sealing additive which can be released in your sample. Filter porosity has been scientifically determined to constitute an effective barrier against aerosols generated by pipetting tasks. Proven high efficiency. Sterile and non sterile versions are autoclavable to ensure perfect sterility once the rack has been opened. *ISO 8655 is an international standard for “piston operated apparatus”. ISO 8655 defines, among other things, terms and definitions regarding piston operated pipettes and their testing, testing procedure and maximum permissible errors. 24 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com For more details on filter tips, see the application notes insert We performed an evaluation of different filter tip brands over a complete aerosol size range of 0.05, 0.1, 0.3, 0.5 and 1μm and found that these particles behave as the theory of filtration would predict. By further comparison of the results, we also concluded that although these filter tips are considered of the highest quality available on the market, they behave in a different manner. For example, for 1 μm particle, filter B has an efficiency of 81% as opposed to 98% for Gilson Diamond tip. Clean Production Environment To avoid any contamination from human contact, the manufacturing process is fully automated. Experienced personnel is equipped with protective clothing to diminish the contamination risks. All Diamond tips are manufactured and packaged in a clean room. Pipetting Systems, as for all of your laboratory instruments are part of your Quality management. Fully traceable from the mold to your laboratory bench Trace Metal Release Gilson logo means genuine Diamond tips. A certificate giving evidence of the low level of potential contamination is included with Gilson Diamond tips. Identification number guarantees the manufacturing quality of the tips. Sterilized tips Batch number on every pack. Sterility assurance level SAL 10 guaranteed, using gamma irradiation. Tip model and tip volume identification on each pack and rack. All packs are wrapped in medical device quality plastic which provides a complete shield against contamination. Quality certificate in every box -6 Indicator dots used on Gilson products undergo an irreversible color change upon sterilization. Yellow gamma dots turn red when products have been properly exposed. Free of detectable RNases, DNases, DNA, RNA and Proteases. -R egular analysis carried out by an independent laboratory. - Certificate of compliance with detailed protocols and detection levels for each batch. Filter Tip brands: Gilson A B C States that each batch of tips has been sampled and inspected with strict tolerances for straightness, uniformity and performance. Batch number recorded on each certificate. States that all tips comply with the CE IVD directive. Color-coded packaging Volume recognition at a glance. Helps prevent errors in the stock room and in the laboratory. Environmental Policy Gilson is concerned about disposal of plastics into the environment and knows that most of our end users also feel responsible. The company has taken a number of initiatives to minimize the impact of tip-packaging waste and has notably designed an original reload system (Tower Pack™) which uses 70% less plastic than racks. D 100 Filter efficiency (% ) Take a step forward in meeting your quality requirements 95 All plastic parts and cardboard packaging are recyclable and marked with the recycling logo. 90 85 80 75 70 0 0,1 0,2 0,3 0,4 0,5 0,6 0,7 0,8 0,9 1 Aerosol diameter (µm) Filter tips efficiency 25 PIPETMAN Tips DIAMOND Concept ® ® Sophisticated, A large choice of Yetpackaging Simple to Operate to fit your activity and budget Diamond certified tips assure optimal performance of your Pipetman pipette. The only way to ensure precision and reproducibility across the full volume range is to use Diamond precision tips with Pipetman. Tip model Pipette model P = Pipetman P, F = Pipetman F, U = Pipetman Ultra, C = Pipetman Concept, N= Pipetman Neo Tips Volume range D10 P2, P10, , P2N, P10N, U2, U10, C10 0.1 - 10 µL DL10 P2, P10, P2N,P10N, U2, U10, U8x20, U12x20, C10, C8x10, C12x10 0.1 - 20 µL D200 P20 to P200, P8x200, F2 to F200, P20N to P200N U20 to U200, U8x20, U12x20, U8x300, U12x300, C100, C300, C8x100, C12x100, C8x300, C12x300 2 - 200 µL D300 P200, P200N, U200, U8x300, U12x300, C300, C8x300, C12x300 20 - 300 µL D1000 P1000, P1000N, F250 to F1000, U1000 200 - 1000 µL D1200 P1000, P1000N, F250 to F1000, U1000, C1200 100 - 1200 µL D5000 P5000, U5000, C5000 1 - 5 mL D10ml P10ml, U10ml, C10ml 1 - 10 mL DF10 P2, P10, P2N, P10N, U2, U10 0.1 - 10 µL DFL10 P2, P10, P2N, P10N, U2, U10, U8x20, U12x20, C10, C8x10, C12x10 0.1 - 10 µL DF30 P20, P20N, F2 to F25, U20, U8x20, U12x20 2 - 30 µL DF100 P100, P100N, F50 to F100, U100, C100, C8x100, C12x100 20 - 100 µL DF200 P200, P8x200, P200N, F200, U200, U8x300, U12x300, C300, C8x300, C12x300 20 - 200 µL DF300 P200, P200N, U200, U8x300, U12x300, C300, C8x300, C12x300 20 - 300 µL DF1000 P1000, P1000N, F250 to F1000, U1000 200 - 1000 µL Work safely with the Diamond Filter Tips 26 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Tipack Diamond Tips: 10 Tipacks of 96 racked tips Autoclavable Tower Pack: refill system for Diamond Tips 10 racks of 96 tips -Ray sterilized Autoclavable -Ray sterilized SterilPack: 400 tips individually wrapped EcoPack: box of 10,000 tips EasyPack: box of 1,000 tips (10 bags of 1,000) (5 bags of 200) Empty Racks (set of 2) F161632 F161905 F167101 F167201 - F161630 F161631 F161791 F161452 F161455 F167102 F167202 - F161450 F161451 F161393 F161932 F161937 F167103 F167203 F161330 F161930 F161931 F161391 F161732 F161735 F167105 F167205 - F161730 F161731 F161737 F161672 F161975 F161340 F161670 F161671 F161793 F161101 F106104 F161102 F161103 F161370 - (12 Tipacks of 50 tips) F161230 F167104 F167204 (7 racks of 96 tips) (7 racks of 96 tips) - - - F161100 - - - Item Reference Number Universal reload box F167100 - - - (8 Tipacks of 30 tips) F161710 F161633 F161454 F161453 F161935 F161933 F161939 F161938 F161571 F161360 (3 bags of 334) (1 rack) F161210 F161220 (200 tips) (1 rack) D1200 Diamond Tips 100 - 1200 µL New slender and long tip geometry prevents the tip holder from coming in contact with wells and tubes. 1000 µL Designed for use even in long, narrow tubes. Graduations at 100 µL and 1000 µL make it easy F161936 F161934 to visually check the volume pipetted. F161734 F161733 F161770 F161673 100 µL Specially designed for Gilson Pipetman Concept C1200. Compatible with Pipetman P1000 and Pipetman Ultra U1000. 27 MICROMAN ® The Positive Displacement Pipetting Reference Improve accuracy and reliability with problem liquids How does it work ? 1. No air-to-liquid interface Perfect solution for complete and rapid pipetting of viscous and dense liquids such as oil, syrup, cosmetic cream, liquefied food, paint, glycerol or buffers. 2. The desired volume is aspirated rapidly and completely Unique solution to avoid leakage when pipetting high vapor pressure liquids such as acetone, chloroform, alcohol, hexane or solvents. 3. The positive wiping action of the piston against the capillary wall assures accurate dispensing of even the most viscous sample and avoids any carry-over 1 2 % Random error 3 3.5 3 Regular Air displacement Pipette 2.5 2 1.5 1 0.5 MICROMAN ® 0 0 40 70 80 85 90 Viscosity/Density 95 5 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 100 % Glycerol % Random error % Systematic error 3.5 3 Regular Air displacement Pipette Regular Air displacement Pipette 2.5 2 1.5 1 MICROMAN ® 0 40 60 80 85 90 95 Viscosity/Density 100 % Glycerol 0.5 MICROMAN® 0 0 40 70 80 85 90 Viscosity/Density 95 100 % Glycerol Reference: Gilson S.A.S. Further details available at www.gilson.com 100% protection against contamination Disposable capillaries and pistons prevent contact of the pipette with corrosive vapors or liquids. Built-in tip ejector prevents user contamination. No air-to-liquid interface protects the sample from aerosol contamination. Contamination-free pipetting of liquids such as DNA fragments, PCR templates, enzyme solutions or radio labeled components. For more details on Microman, see the application notes insert Unprotected air space: risk of aerosol contamination Regular Air Displacement Pipette 28 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Protected air space, no aerosol contamination no vapors Microman Positive Displacement Pipette 5 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 %S Microman and Capillary Pistons (CP) Microman Capillary Pistons Capillary Piston Microman Reference Model number Quantity/ Sterilized Assembled Box Large volume range from 1 µL to 1000 µL with six different models. CP10 M10 F148412 192 CP10 M10 F148312 No need for calibration when you change the CP. CP10ST M10 F148413 CP10ST M10 Continuous volume adjustment and direct digital display. CP25 CP50 Disposable CP, capillaries made of pure polypropylene. Free of dyes, no risk of contamination from the CP’s components. Slim CP fits in even the narrowest and deepest tubes. Racked in Tipack No Yes Yes 960 No Yes Yes 192 Yes Yes Yes F148313 960 Yes Yes Yes M25 F148112 200 No No No M50 F148113 200 No No No CP100 M100 F148414 192 No Yes Yes CP100 M100 F148314 960 No Yes Yes CP100ST M100 F148415 192 Yes Yes Yes CP100ST M100 F148315 960 Yes Yes Yes CP250 M250 F148114 200 No No No CP1000 M1000 F148560 182 No Yes Yes CP1000ST M1000 F148180 182 Yes Yes Yes Microman Models Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Model Capillary Piston Reference Number Volume (μL) Systematic error (μL) Random error (μL) Systematic error (μL) Random error (μL) M10 CP10 F148501 1 5 10 ± 0.09 ± 0.10 ± 0.15 ≤ 0.03 ≤ 0.03 ≤ 0.06 ± 0.20 ± 0.20 ± 0.20 ≤ 0.10 ≤ 0.10 ≤ 0.10 M25 CP25 F148502 3 10 25 ± 0.25 ± 0.27 ± 0.30 ≤ 0.08 ≤ 0.08 ≤ 0.10 ± 0.70 ± 0.70 ± 0.70 ≤ 0.30 ≤ 0.30 ≤ 0.30 M50 CP50 F148503 20 50 ± 0.34 ± 0.70 ≤ 0.20 ≤ 0.30 ± 0.70 ± 0.70 ≤ 0.30 ≤ 0.30 M100 CP100 F148504 10 50 100 ± 0.50 ± 0.75 ± 1.00 ≤ 0.20 ≤ 0.30 ≤ 0.40 ± 1.50 ± 1.50 ± 1.50 ≤ 0.60 ≤ 0.60 ≤ 0.60 M250 CP250 F148505 50 100 250 ± 1.50 ± 1.70 ± 2.50 ≤ 0.30 ≤ 0.30 ≤ 0.50 ± 6.00 ± 6.00 ± 6.00 ≤ 2.00 ≤ 2.00 ≤ 2.00 M1000 CP1000 F148506 100 500 1000 ± 3.00 ± 5.00 ± 8.00 ≤ 1.60 ≤ 2.50 ≤ 4.00 ± 12.00 ± 12.00 ± 12.00 ≤ 4.00 ≤ 4.00 ≤ 4.00 • Microman Pipettes are available with a calibration certificate issued by an ISO 17025 accredited laboratory. (See pages 18-19). 29 DISTRIMAN and DistriTips ® ® Simplify Repetitive Pipetting Distriman is a positive-displacement, continuously adjustable repeater-pipette to simplify multiple dispensing when used with Gilson DistriTip syringes. Direct volume readout With the Distriman you can dispense the exact volume, eliminating tedious calculations. Only 3 Syringes are needed Micro 125 µL, Mini 1250 µL and Maxi 12.5 mL cover the entire volume range (aliquots from 1 µL to 1.25 mL). Distriman is the only repetitive pipette available capable of dispensing more than 100 aliquots. Wide selection of packaging options Each model of DistriTip is available in standard or sterilized (ST) version, in boxes of 50 syringes. Sterilized DistriTips are individually wrapped and certified free of RNase, DNase, DNA, RNA and protease. Distriman Reference Number F164001 DistriTips (box of 50 syringes) Model Aliquot Range 125 µL Micro Micro ST* 1 µL to 12.5 µL 1250 µL Mini Mini ST* 10 µL to 125 µL 12.5 mL Maxi Maxi ST* 100 µL to 1.25 mL Gilson Maximum Permissible Errors Reference Number F164100 F164130 F164110 F164140 F164120 F164150 Volume (µL) Min. Random error (µL) Systematic error (µL) Random error (µL) 2 ± 0.100 ≤ 0.080 ±0.20 ≤ 0.10 5 ±0.125 ≤ 0.075 ±0.20 ≤ 0.10 Max. 10 ±0.200 ≤ 0.100 ±0.20 ≤ 0.10 Min. 20 ±0.80 ≤ 0.20 ±1.50 ≤ 0.60 50 ±1.00 ≤ 0.40 ±1.50 ≤ 0.60 Max. 100 ±1.00 ≤ 0.60 ±1.50 ≤ 0.60 Min. 200 ±6.00 ≤ 1.00 ±12.00 ≤ 4.00 500 ±7.50 ≤ 1.50 ±12.00 ≤ 4.00 1000 ±10.0 ≤ 2.50 ±12.00 ≤ 4.00 Max. *ST means sterilized syringes (individually wrapped). 30 Systematic error (µL) ISO 8655 Maximum Permissible Errors Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com REPETMAN and Repet-Tips ® Rapid, Reliable, Repetitive, Motorized Dispensing High performance Comfortable Wide range of dispensed volume. From 1 µL to 50 mL including non-standard such as 7.46 µL, 156 µL or 12.54 mL. Simple and intuitive operation. Three operating modes: Dispensing, Automatic Dispensing and Pipetting. Compatible with most standard syringe tips. Brand PD-Tip™, Eppendorf® Combitips® plus, etc. Rechargeable battery pack. Automatic battery recharging by placing Repetman on the charging stand. A second battery pack can be charged directly on the stand. Ergonomic design. Fits naturally in either hand and increases operator comfort. Automatic tip identification. Coded syringe-plunger automatically identifies the tip size. Learn function. Digital pipetting-rate monitoring technology detects your true pipetting-rate and then automatically adjusts dispensing rhythm. Large choice of Repet-Tip packaging Eight standard or sterile Repet-Tip syringe sizes available from 0.1 mL to 50 mL. Sterile Repet-Tips are individually wrapped and certified free of endotoxins and pyrogens. Repetman* Description Ref. Number Repetman, EU plug (230V / 50Hz) F164501 Repetman, US plug (110V / 50-60Hz) F164502 Repetman, UK plug (230V / 50Hz) F164503 Repetman, Australian plug (240V / 50Hz) F164504 NiMH battery pack F164595 *Currently not available in the US and Canada Repet-Tips Model Non-sterile Sterile* Repet-Tip (0.1 ml, box of 100) F164510 F164515 Repet-Tip (0.5 ml, box of 100) F164520 F164525 Repet-Tip (1.25 ml, box of 100) F164530 F164535 Repet-Tip (2.5 ml, box of 100) F164540 F164545 Repet-Tip (5.0 ml, box of 100) F164550 F164555 Repet-Tip (12.5 ml, box of 100) F164560 F164565 Repet-Tip (25 ml, box of 50)** F164570 - Repet-Tip (25 ml, box of 25)** - F164575 Repet-Tip (50 ml, box of 25)** F164580 F164585 Adapter for 25/50 ml Repet-Tip (set of 10) F164591 - Adapter for 25/50 ml Repet-Tip (set of 5) - F164592 * Sterile / Endotoxin-free ** One autoclavable adapter is provided in each box 31 PIPETTE Replacement Parts Spare parts manufactured by Gilson are products of intensive research and stringent quality control, guaranteed to deliver value and assure reliability and precision over the long run. Having your pipettes regularly serviced by a Gilson certified and audited service center, staffed by qualified technicians and using genuine Gilson spare parts is the best way to guarantee high performance and trouble-free pipetting. Since 2003, all PIPETMAN tip-holders, pistons and tip ejector heads are embossed with the Gilson trident to protect users from counterfeit products. Gilson spare parts are only available from your local, authorized Gilson representative. Service Kits for Pipetman P Reference Number Item key P2 P10 P20 P100 P200 P1000 P5000 P10 mL Service kit 1st level (1 tip holder, 3 piston seals and 3 O-rings) C+D+E F144501 F144502 F144495 F144496 F144497 F144498 F144499 F144503 Service kit 2nd level (1 push-button, 1 connecting nut, 1 tip ejector and 1 tip ejector adapter only for Pipetman P2/P10) A+B+F F161970 F161971 F161972 F161973 F161974 F161978 * * * Not applicable with this model. Refer to the User’s Guide (LT80117) for details. D E C A B Pipetman P Replacement parts F Reference Number Item key P2 P10 P20 P100 P200 P1000 P5000 P10 mL Push-button assembly Seal & O-ring (5 sets) Tip-holder Adapter for Dual-position Tip ejector (plastic part only, 5 sets) Bags of 10 filters Bags of 100 filters A C+D E ** * F144861 * F144879 * * * F144862 * F144879 * * * F144863 * * * * * F144864 * * * * * F144865 * * * * * F144866 * * * * F144787 F144867 F123608 * F161280 F161241 F161281 F161829 F161263 * F161280 F161241 ** * Not applicable with this model. **Not shown. Refer to the User’s Guide (LT80117) for details. Pipetman F Reference Number Item Piston assembly Seal & O-ring (5 sets) Connecting Nut Tip ejector Tip holder Service kit 1st level (1 tip holder, 3 piston seals and 3 O-rings) Adjustment key Bags of 10 filters Bags of 100 filters F2 to F20 F25 to F100 F200 F250 to F1000 F5000 F123845 F144863 F144723 F144766 F123353 F144611 F144864 F144723 F144767 F144602 F123846 F144865 F144723 F144768 F123305 F123847 F144866 F144723 F144769 F123371 F123848 F144867 * * F123608 F144495 F144496 F144497 F144498 F144499 F123674 * * F123674 * * F123674 * * F123674 * * F123674 F161280 F161241 * Not applicable with this model. Refer to the User’s Guide (LT801118) for details Pipetman 8X200 Item Push-button assembly Nozzle & Piston assembly (8 sets) Silicon Grease Spring (internal) Cover liquid end box (6 screws) Distriman Reference Number F1861081 F161067 F1485012 F1861072 F1861073 Refer to the User’s Guide (LT801236) for details 32 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Item Push-button assembly Piston retaining assembly Red flag (set of 5) Retaining spring Reference Number F164040 F164070 F164042 F1864052 Refer to the User’s Guide (LT801285) for details L M K J G A B H R I E+D C Q P F N O Pipetman Ultra Reference Number Item key U2 U10 U20 U100 U200 U1000 U5000 U10 mL A B C D E F G H I J K L M ** ** ** ** ** F2070152 F2072102 F20751 F2070158 F20710 F2072107 F2072111 F2070160 F2070161 F2070159 F2070117 F161901 F2070118 F2070903 * * * F144875 F2070252 F2072102 F20751 F2070158 F20710 F2072107 F2072111 F2070260 F2070161 F2070159 F2070117 F161902 F2070218 F2070903 * * * F144875 F2070352 F2072102 F20751 F2070158 F20710 F2072107 F2072111 F2070360 F2070161 F2070359 F2070117 F161908 F2070318 * F2070902 * * F144875 F2070452 F2072102 F20751 F2070158 F20710 F2072107 F2072111 F2070460 F2070161 F2070459 F2070417 F2070401 F2070418 * F2070902 * * F144875 F2070552 F2072102 F20751 F2070158 F20710 F2072107 F2072111 F2070560 F2070161 F2070559 F2070517 F2070501 F2070518 * F2070902 * * F144875 F2070652 F2072102 F20751 F2070158 F20710 F2072107 F2072111 F2070664 * F2070659 F2070617 F2070601 F2070618 * F2070902 * * F144875 F2070752 F2072102 F20751 F2070158 F20710 F2072107 * F2070760 * F2070759 * F2070701 F2070719 * F2070902 F161280 F161241 F144875 F2070852 F2072102 F20751 F2070158 F20710 F2072107 * F2070860 * F2070859 * F2070801 F2070818 * F2070902 F161280 F161241 F144875 Push-button assembly Personalization window*** Recalibration tool*** Battery holder*** Battery, flat 1.5 V*** Battery compartment cover*** Connecting nut*** Piston assembly Spring & Guide Tip ejector assembly Tip holder, upper part O-ring, X5 (or Seal for U2-U10) Tip holder, lower part Tip ejector extension for U2-U10 Lubricant tube «End user version» *** Bags of 10 filters Bags of 100 filters Safety bags for single-channel, batch of 100 * Not applicable with this model. **Not shown *** Suitable for Pipetman Ultra Multi-Channel. Refer to the User’s Guide (LT801441) for details. Pipetman Ultra Multichannel Item Push-button assembly Piston assembly Airtightness Kit Tip holder assembly, G-FIT System Tip holder assembly standard Wire clip Ejector cover kit Reassembly tool Safety bags for multichannel, batch of 100 Reference Number key 8X20 12X20 8X300 12X300 A N O P P Q R ** ** F2073052 F2073060 F2073065 F207306306 F2073063 F207306407 F2073059 F2073001 F2073270 F2073152 F2073060 F2073065 F207306306 F2073063 F207306407 F2073159 F2073001 F2073270 F2073252 F2073260 F2073265 F207326306 F2073263 F207306407 F2073259 F2073201 F2073270 F2073352 F2073260 F2073265 F207326306 F2073263 F207306407 F2073359 F2073201 F2073270 **Not shown. Refer to the User’s Guide (LT801462) for details. Microman Item Button assembly, blue, M10 Button assembly, blue, M100 Button assembly, blue, M25 Button assembly, blue, M50 Button assembly, blue, M250 Button assembly, blue, M1000 Pipetting Aid Reference Number F148568 F148569 F148615 F148616 F148618 F148619 Refer to the User’s Guide (LT801502) for details Item PTFE Filter, 0.2 µm, autoclavable (package of 5) PTFE Filter, 0.45 µm, autoclavable (package of 5) Nosepiece Nosepiece Hanger Adaptor for D5000 and D10mL Diamond® Tips Reference Number F161245 F161246 F161249 F161253 F161287 Refer to the User’s Guide (LT801478) for details 33 PIPETTE Replacement Parts Pipetman Concept Reference Number Item Battery block*** Adjustable hook*** Personalization window*** Connecting nut Piston assembly Return spring Spring guide Tip ejector assembly Tip holder, upper part O-ring, X5 (or Seal for C10) Tip holder, lower part Tip ejector adapter for C10 Lubricant tube «End user version» *** Bags of 10 filters Bags of 100 filters key C10 C100 C300 C1200 C5000 A B C D E F G H I J K ** ** ** ** F3071165 F3071110 F3071103 F2072111 F3071252 F2070113 F307115451 F2070159 F2070117 F161902 F2070218 F2070903 F3070902 * * F3071165 F3071110 F3071103 F2072111 F3071352 F2070113 F307115451 F2070459 F2070417 F2070401 F2070418 * F3070902 * * F3071165 F3071110 F3071103 F2072111 F3071452 F2070113 F307115451 F2070559 F307145302 F307145351 F307145301 * F3070902 * * F3071165 F3071110 F3071103 F2072111 F3071552 F2070113 F307115451 F2070659 F2070617 F2070601 F2070618 * F3070902 * * F3071165 F3071110 F3071103 * F3071652 * * F2070759 * F2070701 F2070719 * F3070902 F161280 F161241 * Not applicable with this model. **Not shown *** Suitable for Pipetman Concept Multi-Channel. Refer to the User’s Guide (LT801489) for details. F G K J I E H A D C P B O N L M Pipetman Concept Multichannel Item Piston assembly Airtightness Kit Tip holder assembly, G-FIT System Wire clip Ejector cover kit Reassembly tool Reference Number key 8X10 12X10 8X100 12X100 8X300 12X300 L M N O P ** F3073254 * F3073255 F207306407 F307325051 * F3073254 * F3073255 F207306407 F307425051 * F3073354 F3073360 F3073355 F207306407 F307335051 F3073301 F3073354 F3073360 F3073355 F207306407 F307435051 F3073301 F3073454 F2073265 F307326306 F207306407 F2073259 F2073201 F3073454 F2073265 F307326306 F207306407 F2073359 F2073201 * Not applicable with this model. **Not shown. Refer to the User’s Guide (LT801489) for details. 34 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com 402 Dilutor-Dispenser Liquid Handling at the Touch of a Button High performance Nine pre-stored tasks that can be linked: dilute, dilute serially, dispense aliquots or variable volumes, pipette, rinse, mix, titrate, measure volume and prime. High volume range from 1µL to 99 mL. Provides the same high precision and accuracy as Gilson pipettes. Minimize costs Adjustable flow rate. Minimal cross-contamination with the adapter kit for tips. Improve productivity Maintenance-free - permanently calibrated. Reduces preparation time and consumption of expensive reagents. Faster than any other pipettor for repetitive liquid transfer. Highly versatile with upgrade possibilities and the tip-adapter kit. Fully programmable: ability to save and recall up to 99 methods. Comfort No need for calculation of dilution ratios. Lightweight probe (48g) and low activation forces make the 402DD even more comfortable than electronic motorized pipettes. Single instrument to perform all liquid transfer tasks. Increase reliability - GLP Menu-driven software. Eliminates technician to technician variability. Model (without syringes, which are sold separately) Coded protection of methods. 402 Dilutor-Dispenser, single-syringe, 110-220V F410514 402 Dilutor-Dispenser, single-syringe, 220V (French version) F410514F Calibration certificate, IQ/OQ procedure. LED indicator for error-free manipulation. No contamination : the probe may be fitted with Diamond Tips. Reference Number 402 Dilutor-Dispenser, dual-syringe, 110-220V F410515 402 Dilutor-Dispenser, dual-syringe, 220V (French version) F410515F Accessories Reference Number Additional Accessories Reference Number Adapter Kit for disposable tips F4015015 25025341 Transfer Tubing, 1 mL, FEP, 1.0 x 2.0 x 1300 mm F4015031 Syringe, 100 µL Syringe, 250 µL 25025342 Transfer Tubing, 2 mL, FEP, 1.5 x 2.0 x 1300 mm F4015032 Syringe, 500 µL 25025347 Syringe, 1 mL 25025343 Transfer Tubing, 5 mL, FEP, 1.5 x 2.1 x 3000 mm F4015033 Syringe, 5 mL 25025344 Transfer Tubing, 10 mL, FEP, 1.5 x 2.1 x 6000 mm F4015034 Syringe, 10 mL 25025345 Syringe, 25 mL 25025346 35 MINIPULS Evolution ® Laboratory Peristaltic pump The Minipuls evolution® is the newest innovation from the makers of the MinipulsTM3, offering unmatched flexibility and performance for liquid Unbeatable flow rate range from 0.2 μL/min to 340 mL/min transfer applications. Stackable pump heads for up to 16 channels Medium and high flow pump heads can be used simultaneously in the same module Reproducibility of flow rate on all channels with a variation ≤ 0.1% 0.01 rpm readability below 10 rpm, perfect for accurate flowrate adjustment Optimised 6-roller design limits pulsations Offers back pressure resistance up to 0.3MPa (42psi) 36 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com How to select the most appropriate configuration? 1 Speed control module 2 Select the pump head(s): MF1 pump head, with 1 cartridge, 0.2µL/min to 70mL/min Ref. F110705 Gilson robustness and reliability MF4 pump head, with 4 cartridges, 0.2µL/min to 70mL/min Ref. F110706 Safe and durable design, can work 24/7. S tainless steel rollers for great durability and chemical resistance. Stack up to 4 MF pump heads together for up to 16 channels The stepper motor and the spring loaded cartridges assure a high precision of flow rate despite tubing stretching: less than 2% variation after 24h in operation. HF2 pump head, with 2 cartridges, 1mL/min to 340mL/min Ref. F110707* Quick, flexible & safe set up Cartridges snap in & out of the pump head for fast tubing change - no need to remove the pump head from the speed control module. Pump heads can be changed within seconds even while pump is operating - no tool required. djustable tubing occlusion for simple and A fine pressure adjustment. Stack up to 3 HF pump heads together for up to 6 channels ED NT PATE ED NT PATE NTED PATE I ntuitive software interface with large LCD screen. Clear setting display on the large LCD screen Start/Stop. Variable flow rate and direction. Prime mode : purge tubing at the touch of the button. * Consult your local distributor for details / availability. Ref. F110701 3 Select the appropriate tubing: • Check the appropriate material according to your sample’s chemical compatibility • 4 types of tubing are available to choose from: - PVC for standard use - Silicone for best biocompatibility - Pharmed® for increased lifetime - conforms to USP & FDA class 4 - Isoversinic® for high chemical compatibility • Check the maximum required flow-rate • Choose tubing internal diameter, connectors and extensions from the table on page 40 4 Select the footswitch pump control for increased comfort Ref. F1077081 Pharmed® and Isoversinic® are registered trademarks of Norton Performance Plastic Corp. 37 PIPETMAN3Concept MINIPULS ™ ® Sophisticated, Pulse-free FlowYet Peristaltic Simple to Pump Operate High performance and versatile Reliable and robust Pulse-free flow (10 stainless steel rollers!). Excellent flow-rate accuracy and reproducibility. High chemical resistance material (drive module and pump heads). Wide flow-rate range from 0.3 µL/min to 220 mL/min. Motor speed stability (high torque stepper motor). Operates at higher pressures than competitor products (up to 0.5 MPa). Built-in overflow system, in case of tubing leakage. Low noise operation. Adjustable cams allow fine flow-rate adjustment to minimize tubing wear. Two-stop calibrated tubing makes it easy to install tubing on the pump head. Max Flow Rate Item Number of channels Reference Number F155001 Pump Module Minipuls 3 R1 Pump Head up to 30 mL/min 1 F117604 R2 Pump Head up to 30 mL/min 2 F117800 R4 Pump Head up to 30 mL/min 4 F117606 R8 Pump Head up to 30 mL/min 8 F117608 R2/HF Pump Head (High Flow) up to 220 mL/min 2 F117830 R4/HF Pump Head (High Flow) up to 220 mL/min 4 F117831 A large choice of suitable tubing is available (see page 40) 38 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Electrophysiology of rat brain cells “ To measure electrical signals and intracellular calcium concentrations of neuronal cells at the same time, Mr. Kwang-Hyun Cho, PhD, a postdoctoral researcher from the College of Medicine at the Catholic University of Korea, uses a microscope focusing on a perfusion chamber drained by a Minipuls 3. The neuronal cells come from a rat brain slice bathed in a buffer inside the chamber. This buffer is an oxygenated saline solution that ensures the viability of the neuronal cells, which are very sensitive to oxygen. Chamber Applications MINIPULS 3 ™ Outlet tubing Inlet tubing The Minipuls 3 is very convenient for the re-circulation of solutions ” Minipuls 3 says Doctor Kwang-Hyun Cho when referring to a key benefit of the pump. Engineered with a 10-roller rotor, the Minipuls 3 produces smooth pulse-free flows, which eliminate siphoning or clogging phenomenon, a significant advantage when circulating sensitive neuronal cells. Chamber MINIPULS Evolution ® Study of bacterial biofilm In the Institute of Biology at the Leiden University, the molecular biology team seeks to identify Pseudomonas (Pseudomonas putida) genes and traits involved in efficient competitive root colonisation in order to understand how bacteria survive, proliferate, and compete in the rhizosphere after bacterisation of seeds. The Minipuls evolution is used to study bacterial biofilms in flowcell chambers. Bacteria grow in flowcell chambers fed by an oxygenated solution and media. The Minipuls evolution is used to bring this solution to the chambers and recycle it. Growth media Oxygenated solution waste tubing inlet tubing outlet bubble trap Flowcell chambers The growth media was delivered at 0.125 mL/min to the flowcell system. Elen Lagendijk, technical assistant at the molecular and biology department, says that one of the outright benefits of the Gilson Minipuls evolution peris- “ taltic pump is that it enables the operator to replace tubing, add or remove channels while the pump is running without interrupting the flow in the other chambers… ” 39 MINIPULS Accessories How to select the right tubing for your Minipuls 3 or Minipuls evolution? A collection of tables and charts is available at www.gilson.com to be used as a guide for selecting the appropriate tubing. Most suitable material according to your sample’s chemical compatibility. Large variety of Internal Diameters to reach desired flow rates. Appropriate connectors and extension tubing. 40 Internal diameter (mm) Reference tubing (calibrated tubing) Minipuls 3 Maximum Flow rate (mL/min) Minipuls evolution Maximum Flow rate (mL/min) 0,25 F117932 F117952 F117985 0,407 0,574 2,06 F117965 24 33 0,38 F117933 F117953 F117985 0,695 1,22 2,29 F117966 29 41 0,51 F117934 F117954 F117985 1,415 1,90 2,79 F117968 41 66 0,76 F117936 F117956 F117986 2,1 3,00 3,16 F117969 49 85 1,02 F117938 F117958 F117986 4,1 6,0 4,0 F117970 75 128 1,30 F117940 F117960 F1179941 6,2 9,0 5,0 F117980 113 179 1,52 F117942 F117962 F1179941 9 14 6,0 F117981 163 218 1,65 F117943 F117963 F1179941 10 16 8,0 F117982 213 340 2,06 F117945 F117965 F1179941 16 25 2,0 F117744 23 30 2,29 F117946 F117966 F1179941 20 29 3,0 F117746 48 67 2,79 F117948 F117968 F1179951 23 39 4,0 F117748 86 120 3,16 F117949 F117969 F1179951 29 47 6,0 F117750 181 246 0,5 F1817741 F117740 F117986 1,8 2,9 2,0 F1825133 25 31 1,0 F1817743 F117742 F117986 4 10 3,0 F117975 44 57 2,0 F1817745 F117744 F1179941 13 24 4,0 F117976 71 105 3,0 F117747 F117746 F1179951 27 55 5,0 F117977 115 148 4 F117749 F117748 - 33 70 6,0 F117978 150 207 0,6 F1825111 F1825131 F117986 1,5 3,2 7,0 F117979 220 300 1,0 F1825112 F1825132 F117986 4 7 2,0 F1825123 26 31 2,0 F1825113 F1825133 F1179951 15 21 3,2 F1825124 57 77 2,8 F1825114 F117975 F1179951 23 31 6,4 F1825125 171 240 0,5 F1825101 F1825121 F117986 1,4 2,5 1,0 F1825102 F1825122 F117986 5 7 2,0 F1825103 F1825123 F1179941 15 24 Color coded stops Minipuls evolution Maximum Flow rate (mL/min) PHARMED® Minipuls 3 Maximum Flow rate (mL/min) SILICONE Connectors ISOVERSINIC® Extension tubing (3m/118in) PVC Reference tubing (calibrated tubing) Material Tubing for R2HF, R4HF & HF2 high flow pump heads (length = 3m / 118in ) Internal diameter (mm) Tubing for R1, R2, R4, R8, MF1 & MF4 pump heads (length = approx 40 cm/ 16in) Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Material PVC ISOVERSINIC® SILICONE PHARMED® Pharmed® and Isoversinic® are registered trademarks of Norton Performance Plastic Corp. GILSON Safe Aspiration Station The Best Solution for Safe Liquid Aspiration Comfort & flexibility Safe & contamination free Quiet operation Motor starts only when vacuum pressure drops. Closed, stand-alone unit designed to prevent contamination hazards. Compact design and small footprint Allows for efficient use of space in laminar flow cabinets. All parts in contact with the liquid are autoclavable. Adjustable aspiration speed allows for quick and accurate aspiration. Level detection to prevent overflow. In-line hydrophobic filter traps aerosols. Handle accommodates different types of pipettes to aspirate from a variety of vessels. Always ready to use Just press the handle-button to aspirate. Convenient coupling for quick bottle emptying. Cell culture Cell culture Unbreakable bottle. Connect two handles to the Gilson Safe Aspiration Station with the Y-shape connector. Description Reference Number Gilson Safe Aspiration Station F110741 Gilson Safe Aspiration Kit F110742 Y-shape Connector F1077418 ELISA test Liquid aspiration (supernatant, etc.) Gilson Safe Aspiration Kit •1 handle + tubing set + 1 holder •1 stainless steel needle adaptor •1 tip* adaptor •1 tip adaptor with tip* ejector •8-needle stainless steel adaptor •8-tip adaptor with tip* ejector * Compatible with Diamond®tips : DL10, DFL10, DF30, DF100, D200, DF200, D300, DF300 41 PIPETMAN Concept GmCLab ® Sophisticated, Quick and SafeYet Spin Simple with Your to Operate Personal Mini-Centrifuge GmCLab is a fixed-speed personal mini-centrifuge that quickly separates particles from the supernatant in a solid-liquid mixture or spin down liquids to the bottom of a microtube. Compact design Small footprint allows for easy integration on a busy workbench. Accommodates 8 micro-tubes of 1.5 or 2.0 ml, 2 strips of 8-PCR (0.2 ml) tubes or 16 single PCR tubes. Simple to use Simple spin activation by closing the lid. Pinch-type connector to quickly exchange rotors - no tool needed. Lid opens up to an angle of 160° to allow easy access to the tubes. Dynamic brake for emergency stop. Robust Reinforced lid hinge cylinders. Maintenance-free. One-year warranty. GmCLab Description 42 Reference Number GmCLab, EU plug (230 V / 50-60 Hz) F110731 GmCLab, US plug (120 V / 50-60 Hz) F110732 GmCLab, UK plug (230 V / 50-60 Hz) F110733 GmCLab, AU plug (230 V / 50-60 Hz) F110734 GmCLab, JP plug (100 V / 50-60 Hz) F110735 Rotor for 1.5/2.0 ml tubes F1077303 Rotor for 8-PCR tube strips F1077304 Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com LabStir Quality Mixing with an Ultra-Flat Stirrer Ultra-flat design Quality Ultra flat: only 12 mm thick, use is only limited by the container height. Small footprint. Ultra light (0.3 Kg). Easy to move and to store where space is limited. High performance Quality mixing with the alternating stirring mode (forward / reverse every 30 sec.). Friction-free drive technology minimizes heat transfer to the solution. Compact design with no moving parts inside for a robust and reliable module. Maintenance-free. Three-year warranty. Safe to use Impervious to dust and splashes. Safe in Class II explosive environments. CE certified, cULus certified. Accommodates containers up to 800 mL and 90 mm (3.54 in.) in diameter. LabStir Model Variable speed control (15 - 1500 rpm) for efficient mixing, even for solutions of medium viscosity. Reference Number LabStir (100 to 240V / 50-60Hz), EU plug F110711 LabStir (100 to 240V / 50-60Hz), US plug F110712 LabStir (100 to 240V / 50-60Hz), UK plug F110713 GVLab Clearly Simple and Functional GVLab is a “touch-on” fixed speed vortex mixer that quickly mixes solutions or media Compact design. Just touch the container against the pad for instant vigorous and uniform vortexing action. Accommodates any size of tubes and vials up to 30 mm (1.18 in.) diameter. contained in various formats of tubes or vials. Quality GVLab Model Reference Number Sturdy base and housing for stability and minimal vibration during use. GVLab (100 to 240V / 50-60Hz) EU plug F110721 GVLab (100 to 240V / 50-60Hz) US plug F110722 Special tube pad design prevents liquids from damaging the electronics. GVLab (100 to 240V / 50-60Hz) UK plug F110723 Maintenance-free. Three-year warranty. GVLab (100 to 240V / 50-60Hz) AU plug F110724 CE certified, cULus certified. 43 Services Gilson Service network of trained distributors Pipettes are precision instruments used daily in routine laboratory work and often involved in critical laboratory applications. Like any other instrument they need to be serviced and calibrated regularly to ensure that they perform to the highest standards. In line with the ISO 8655, Gilson recommends All Gilson technicians are highly qualified after extensive training by Gilson at the factory and share Gilson’s experience. Their skills are continuously improved for new or upgraded products. Gilson Service Centers are routinely audited to check their abilities. disassembling, cleaning, replacement of worn parts Gilson preventive maintenance - repair & calibration services and checking of the calibration every 6 months or at least once a year. Gilson is a worldwide network of specialists present in more than 50 countries offering a breadth of services to address the demanding requirements of international standards, Good Laboratory Practices and internal procedures. Ultimately, we work to provide you with flexible solutions to extend pipette lifetime and assure you the highest level of continued accuracy and precision at a minimum cost. Pipettes are a staple of most laboratories and probably one of the most extensively used pieces of equipment. The pipette is made of a variety of mechanical and/or electronic parts moving against each other that are often exposed to corrosive or hazardous chemicals. This exposure may result in barely visible damage that may not be discovered unless regular service has been carried out. For example: • The pipette has been tested on the balance, and is imprecise and/or inaccurate. • The pipette leaks after a long period of use. • The pipette leaks after having been exposed to contaminating liquids. • The piston seal and/or O-ring are worn out. • The stainless steel piston is scratched. Gilson Service Center gives you the priority • No surprises, you pay for what is on the estimate • We propose various service offers adapted to your budget • We guarantee that Gilson genuine spare parts are used in all repairs • We guarantee that the personnel is regularly audited and the equipment checked daily 44 • The tip-holder or other parts (tip-ejector, push button, connecting nut, etc) are damaged, chemically attacked or worn. Servicing your pipette regularly will provide you peace of mind regarding its performance and reliability. Gilson offers flexible service solutions that suit your environment: • Diagnosis, repairs, and calibration. • Preventive maintenance contract to implement the level of service most appropriate to your needs (what, how often, number of pipettes, cycle time, etc.). • Calibration by gravimetric method to check the performance of your pipettes according to the ISO 8655 standard, including ISO17025 calibration with the Calibration certificate delivery, adapted to your budget (see page 18). Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com On-Site service Gilson’s qualified technicians are available to visit your facility for any of the following: • Diagnosis: Check that your Gilson pipettes function correctly and are mechanically sound. •R epairs: Replacement of genuine spare parts. • Upgrade of Pipetman: Systematic replacement of key components with upgraded parts. • Pipette Clinics: Servicing of all Gilson pipettes. Servicing may include cleaning, decontamination, repair, (adjustment if needed) and calibration. The “Center of Excellence” label With a clear focus on developing the quality of service to the Gilson pipette users, the company launched the “Center of Excellence” audit campaign in 2005. The “Center of Excellence” label rewards the centers that fully fulfill all Gilson’s requirements. While no detail is overlooked, the main objective of the audits is to accompany the entire Gilson network towards excellence of their service. Audits provide guidance to support the implementation of the necessary means to achieve excellence. 1. U ndergo regular Gilson audits on technical aspects, personnel skills, service quality and service offers. 2. Follow regular training. 3. Repair and calibrate in accordance with Gilson’s servicing procedures or procedures validated by Gilson. 4. Repair the entire Gilson pipette range. 5. Repair Gilson pipettes with genuine spare parts. 6. Monitor environmental conditions. 7. Use correct balances (including 6-digit balance to calibrate volumes < 20 µl). 8. Maintain a comprehensive Service offer that includes: a. D iagnosis, repairs, and calibration by gravimetric method (ISO 8655 Standard). b. Maintenance. c. On-site pipette service. 9. Comply with local standards and regulations. 10. Refuse sales of Gilson spare parts to unauthorized companies. Companies that have successfully completed the Gilson’s recommendation program may use the Center of Excellence label as a graphic symbol to let clients know they have demonstrated the expertise required to diagnose, repair and calibrate. The Center of Excellence Certificate and the use of the numbered label ensure the highest quality of Gilson service and guarantee that the Service Center performs repairs and calibration according to Gilson’s requirements: 45 Services Gilson training* Gilson’s distributors conduct comprehensive seminars to train you or your personnel. Training programs cover all aspects of service and operation of your pipettes. Gilson’s local representatives have completed extensive training in all aspects of service and operation of Gilson products and services. Your local distributor can offer you extensive pipette training covering topics such as: • Good handling practices, gesture and posture. • Use and applications. • GLP requirements, quality control, applicable standards, traceability, and record keeping. • Preventive maintenance and calibration. Do-it-yourself performance check: Weighing Kit The weighing kit is intended for end-users’ use to check the performance of their Gilson pipettes across the full range of volumes from 1 μL to 10 mL. It consists of : •4 different weighing containers, for receiving the dispensed liquid on the balance pan during calibration, • 3 reservoirs used to hold the distilled water during the calibration procedure, • filters, • tweezers, • and a User’s Guide, suitable for the gravimetric method according to ISO 8655 standard. Contact your nearest Gilson Service Center for availability of these offers. *Service offer listed above and offered by Gilson may differ country by country. Please contact your local distributor for details. Pipetman® P Service Pack* Pipetman Service pack* includes a Pipetman P pipette with a two year service offer. No paperwork, no time is wasted waiting for estimates or purchase order numbers. Just return the coupon with your pipette to your local Gilson Distributor to maintain your pipette performance at an economical price. In every Pipetman P Service Pack, you get the pipetting standard along with Gilson’s service expertise for two years. Each Service Pack contains: Description Reference Number Weighing Kit F144700 10 Filters for Weighing Kit F123854 • One Pipetman P pipette, accessories and User’s Guide. • Coupon for FREE service in the FIRST year after purchase. Reference Number Pipetman Service Pack P2 F144803 • Coupon for half-price servicing in the second year. Pipetman Service Pack P10 F144804 Pipetman Service Pack P20 F123610 • Extended two-year warranty from the date of purchase Pipetman Service Pack P100 F123625 Pipetman Service Pack P200 F123611 Pipetman Service Pack P1000 F123612 Pipetman Service Pack P5000 F123613 Pipetman Service Pack P10ml F161203 *Not available in all countries. Please contact your local Gilson distributor for more information. 46 Description Manual Liquid Handling Catalog 2007 / January - More information available at www.gilson.com Microman® positive displacement pipette is now Hydrochloric acid Pipetman® Neo is an evolution of the classic available with a calibration certificate issued by the Gilson ISO 17025 accredited laboratory. The Gilson ISO 17025 accredited laboratory in France provides the undisputable guarantee and confidence that our Quality System and Standard Operating Procedures have been thoroughly evaluated and approved by a legitimate third party. Hydrofluoric acid Formic acid Nitric acid Phosphoric acid Propionic acid Gilson Safe Aspiration Station® is a stand-alone Sulfuric acid vacuum system that incorporates all the essential characteristics to aspirate quickly and safely all kinds of liquids and collect them in a safe container. Trifluoroacetic acid GmCLab is a compact and highly dependable ISO9001 Certified personal mini-centrifuge that quickly separates particles from the supernatant in a solid-liquid mixture or spin down liquids to the bottom of a tube. Gilson Reagent Reservoirs are ideal for dispensing reagent with multichannel pipettes. Two different types of reservoirs are available, 25 mL and 50 mL, standard or sterilized. Minipuls Evolution® laboratory peristaltic pump is the newest innovation from the makers of the Minipuls™3, offering unmatched flexibility and performance for liquid transfer applications. ISO17025 Accredited Benzyl alcohol Aniline Butanol / Butyl alcohol Chloroform Cyclohexane Diacetone alcohol Methylene chloride Diethylene glycol Dimethylformamide (DMF) Dimethylsulfoxide (DMSO) Dioxane (1,4) Ethanol Ether Formaldehyde Hexane Hydrogen peroxide Ammonium hydroxide Sodium hydroxide Sodium hypochlorite Methanol Methyl ethyl ketone Pentane Tetrahydrofuran (TH-) Urea 20% 50% 100% 10% 20% 37% 20% 40% 100% 10% 30% 65% 20% 85% 50% 100% 20% 50% 95% 20% 80% 100% 50% 20% 10% 40% 15% Cl PET = Polyethylene Terephthalate Nitril = Nitril EPDM = Ethylene Propylene LCP = Liquid Cristal Polymer PA = Polyamide PBT = Polybutylene Terephthalate Steel ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ PET N/A + + N/A ++ ++ ++ ++ + + + N/A ++ + N/A N/A ++ ++ + N/A N/A N/A ++ ++ ++ ++ + N/A ++ N/A ++ ++ ++ ++ N/A N/A ++ + N/A ++ ++ N/A ++ ++ Nitril ++ + + + ++ + + + + + + ++ ++ + ++ ++ ++ ++ ++ + ++ ++ + + ++ ++ + N/A EPDM ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ N/A N/A + + N/A N/A N/A N/A ++ ++ N/A ++ + N/A + ++ + ++ ++ ++ ++ ++ ++ ++ + + N/A PC = Polycarbonate PE = Polyethylene PVDF = Polyvinylidene fluoride TPX = Polymethylpentene POM = Polyoxymethylene PP = Polypropylene LCP N/A ++ ++ + ++ ++ + ++ ++ ++ + ++ ++ ++ + N/A N/A N/A N/A ++ ++ N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A ++ ++ N/A N/A ++ N/A N/A + N/A N/A ++ ++ ++ + ++ N/A + - PA ++ ++ ++ N/A ++ ++ + + + + ++ ++ + ++ N/A N/A ++ + ++ ++ ++ ++ ++ ++ N/A ++ ++ ++ ++ ++ N/A ++ ++ PBT N/A ++ ++ N/A N/A N/A N/A ++ + + + ++ + ++ ++ ++ ++ ++ + N/A N/A N/A N/A N/A ++ N/A N/A N/A ++ N/A ++ ++ ++ N/A ++ ++ + + + ++ ++ ++ N/A + ++ PC N/A ++ ++ + ++ ++ + ++ + ++ ++ + ++ ++ + ++ ++ + ++ + ++ ++ N/A N/A ++ ++ ++ ++ ++ ++ + ++ N/A PE ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ + ++ + ++ N/A + ++ ++ ++ ++ ++ + ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ PVDF N/A ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ + ++ ++ N/A + ++ ++ ++ ++ ++ N/A ++ ++ ++ ++ ++ + ++ ++ No chemical degradation + M edium resistance to chemical agents - L ow resistance to chemical agents N/A No data available TPX N/A + + N/A ++ ++ + ++ ++ ++ ++ ++ N/A ++ ++ ++ ++ ++ N/A N/A ++ ++ ++ N/A N/A N/A ++ N/A N/A + + N/A ++ ++ ++ N/A N/A ++ + ++ + ++ ++ ++ ++ ++ ++ + + + N/A POM ++ N/A + N/A ++ ++ + ++ + + + + + + + ++ + + ++ + ++ N/A ++ ++ ++ N/A ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ N/A ++ PP ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + ++ + ++ ++ N/A + ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ N/A + ++ Chemical compatibility table Pipetman P that addresses the growing susceptibility of pipette users to repetitive strain injuries. Product Acetamide Ethyl acetate Acetone Acetonitrile Acetic acid 47 Distributed by Manual Liquid Handling Catalog 2007/2008 Mexico Guadeloupe Martinique Venezuela Guyana Colombia Ecuador Peru Brazil Bolivia Chili Uruguay Japon South Korea China Taiwan Hong kong India Thailand Vietnam Malaysia Singapore Indonesia Australia Gilson Manual Liquid Handling USA Russia New Caledonia Argentina www.gilson.com I [email protected] I [email protected] I [email protected] Gilson, Inc. World Headquarters I 3000 Parmenter Street I P.O. Box 620027 I Middleton, WI 53562-0027 USA Tel: (1) 800-445-7661 or (1) 608-836-1551 I Fax: (1) 608-831-4451 Gilson S.A.S. I 19, avenue des Entrepreneurs I BP 145, F-95400 Villiers-le-Bel, France Tel: (33-1) 34 29 50 00 I Fax: (33-1) 34 29 50 20 LT800486E - © 2007 Gilson S.A.S. - All rights reserved - Printed in France, January 2007. Specifications subject to change without notice. Gilson, Inc. and Gilson S.A.S are ISO 9001 certified. oxygenemultimedia.fr New Zealand 2007/2008 Canada 14 1 Austria 10 2 Belgium 26 8 3 Bosnia and Herzegovina 19 7 18 Belarus 4 Bulgaria 15 31 21 23 5 Czech Republic 2 12 5 25 32 17 6 Croatia 9 30 1 13 24 27 6 3 29 7 Denmark 16 20 4 8 Estonia 22 28 11 Turkey 9 France Malta Cyprus 10 Finland Kuwait Tunis Israel 11 Greece Morocco Jordan Bahrain Pakistan 12 Germany Qatar Egypt 13 Hungary Saudia UAE Oman 14 Iceland Arabia 15 Ireland 16 Italy Senegal 17 Luxembourg 18 Lithuania Nigeria 19 Latvia 20 Macedonia Cameroon 21 Netherlands Kenya 22 Portugal 23 Poland Tanzania 24 Romania 25 Slovakia 26 Sweden 27 Slovenia Iles Maurice 28 Spain Reunion 29 Serbia & Montenegro 30 Switzerland South Africa 31 United Kingdom 32 Ukraine