Pelargonium - Agritrop

Expression of an Arabidopsis
Aspartic protease
in Pelargonium
Mattheu Chabannes
CIRAD Département BIOS
Eva Marazuela
Fundación PROMIVA
Marisé Borja
Universidad Complutense de Madrid/Fundación PROMIVA
CRISP Project HPRN-CT-2000-00093
Phenotypic alterations of over-expressing
CDS10 Arabidopsis plants
Wt plants
CDS10 plants
Smaller rosette
Smaller and
asymmetric leaves
Two independent CDS10 flowering plants
Bushy, multi-branching phenotype
Plants are always dwarf (10 cm high maximum)
Pelargonium Boda Gitana Salmon biolistic
transformation
Embryogenesis regeneration
Pelargonium Boda Gitana Salmon biolistic
transformation
NPTII selection
CDS10-Boda Gitana Salmon
Bushy, multi-branching phenotype is observed in vitro
Boda Gitana Salmon plants
Wt
CDS10 over-expression
Bushy, multi-branching phenotype is observed ex vitro
Boda Gitana Salmon leaves
- No variations are
observed in the shape
and size of the leaves
- Peduncles are longer
and thinner
Wt
CDS10 over-expression
Boda Gitana Salmon flowers
Wt
CDS10 over-expression
- Number of petals is reduced to 5 like in simple varieties
Pelargonium biolistic transformation
Mirada Pink
Mirada Violet
Pelargonium biolistic transformation
Mirada Pink
Mirada Violet
Pelargonium Mirada violet biolistic
transformation
NPTII selection
Pelargonium Mirada violet
Wt
CDS10 over-expression
Pelargonium Mirada Pink
Wt
CDS10 over-expression
Pelargonium Mirada Pink-Wild Type
Pelargonium Mirada PinkCDS10 over-expression
Pelargonium Mirada Violet
CDS10 over-expression
Mirada Pink and Violet flowers
Mirada Pink
Wt
CDS10 over-expression
Mirada Violet
Wt
CDS10 over-expression
- Number of petals is reduced to 5 in both varieties
Wild type vs CDS10 overexpressing lines
Number of
branches
Number
of petals
height (cm)
Wt
5.57
21.66
71.3
T-131
13.00
5
56.8
T-129
15.57
5
59.1
T-147
12.10
5
63.4
T-138
10.9
5
65.2
T-140
9.6
13.39
67.2
Wt
4.36
12.32
53.2
T-22
11.71
5
49.6
Wt
4.86
5
51.3
T-7
12.31
5
49.8
Line
Boda Gitana
Salmon
Mirada Violet
Mirada Pink
Conclusions
+ The over expression of At -CDS10 in Pelargonium transgenic
lines induces an increase in the number of branches, longer
pedicels and a reduction in plant height.
+ At-CDS-10 over expression also induces the decrease in the
number of petals in two double flowered varieties transgenic
plants .
+ Even if the original A. thaliana busy phenotype was of interest
for getting a higher number of cuttings/plant and a compact
phenotype, the pleiotropic effects of the over-expression of the
CDS 10 gene on the flowers are non favourable in the case of
Pelargonium.
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