ProcartaPlex Brochure

ProcartaPlex™
Multiplex Immunoassays
Table of Contents
1.ProcartaPlex Multiplex
Immunoassays1
TM
eBioscience, an Affymetrix company, is committed to
developing and manufacturing high-quality, innovative
reagents using an ISO certified process. As a provider of
more than 11,000 products, we empower our customers
2.Technology Overview
2
worldwide to obtain exceptional results by using reagents
xMAP® Technology . . . . . . . . . . . . . . . . . . . . . 2
that offer a new standard of excellence in the areas of
Luminex® Instrumentation . . . . . . . . . . . . . . . . 2
innovation, quality and value.
ProcartaPlex™ Immunoassay Workflow . . . . . . . 3
3.Product Focus: ProcartaPlex 4
TM
Th1/Th2 T Helper Cell Cytokine Panels��������������4
Tfh/Th9/Th17/Th22/TReg T Helper Cell
Cytokine Panels . . . . . . . . . . . . . . . . . . . . . . . . . . 6
4. ProcartaPlex Multiplexing
Immunoassay Products
TM
8
Human . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Mouse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5. Procarta Mix&Match
®
11
Human . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Mouse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Non-Human Primate . . . . . . . . . . . . . . . . . . . . 12
Rat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Canine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Porcine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Unless indicated otherwise, all products are For Research Use Only.
Not for use in diagnostic or therapeutic procedures.
All designated trademarks used in this publication are the property of their
respective owners.
©Affymetrix, Inc. All rights reserved. Affymetrix®, Axiom®, Command Console®,
CytoScan®, DMET ™, GeneAtlas®, GeneChip®, GeneChip-compatible™, GeneTitan®,
Genotyping Console™, myDesign™, NetAffx®, OncoScan™, Powered by Affymetrix™,
PrimeView®, Procarta® and QuantiGene® are trademarks or registered trademarks of
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InstantOne ELISA™, OneComp eBeads™, ProcartaPlex™, Ready-SET-Go!®, SAFE™ Super
AquaBlue®, The New Standard of Excellence® and UltraComp eBeads™ are trademarks
or registered trademarks of eBioscience, Inc. Instant ELISA® is a registered trademark of
Bender MedSystems, GmbH. FlowLogic® is a registered trademark of Inivai Technologies.
ProcartaPlex Multiplex
Immunoassays
TM
1
What would the ability to simultaneously quantitate 50 cytokines or other soluble
biomarkers from a single 25 μL sample do for your research? The use of multiplex
immunoassays for multiple analyte detection has proven to be an invaluable tool for the
comprehensive study of biological systems. As these systems are comprised of networks
of secreted proteins, including cytokines, chemokines, growth factors and other proteins,
multiplex assays are an efficient method for biomarker profiling of a large set of proteins
from a small sample.
ProcartaPlex Multiplex Immunoassays use the Luminex® xMAP (multi-analyte profiling)
technology that enables the simultaneous detection and quantitation of up to 50 protein
targets in a single 25-50μL sample. The technology employs the use of differentially dyed
capture beads for each target to allow for a multiplex “ELISA-like”assay. ProcartaPlex
allows you to profile up to 50 times more analytes using significantly less sample in the
same time that it takes to perform an ELISA. Refer to pages 2-3 for a technology overview
and assay workflow principles.
ProcartaPlex Multiplex Immunoassays are available in multiple formats across six species (Human,
Mouse, Rat, Non-Human Primate, Porcine and Canine) to meet the needs of your research.
ProcartaPlex Panels—Pre-configured biologically-defined and disease-defined

panels using magnetic beads for the quantitative multiplex analysis of 6 to 45 analytes
in a single sample of plasma, serum, cell culture supernatant, or other bodily fluids.
ProcartaPlex Simplex—Magnetic bead sets to detect individual analytes that are

designed to be added to ProcartaPlex panels to increase customization. Alternatively,
multiple ProcartaPlex Simplex sets can be combined and run using the ProcartaPlex
Basic Kit which includes all non-target specific reagents needed to perform the
ProcartaPlex assay.
Procarta® Mix&Match Assays—Custom blended and optimized panels to deliver

results that are tailored to the panel design of YOUR choice and your sample type.
Simply select the desired analytes from a Procarta Mix&Match list, then select sample
type, bead type, and instrument type. A custom assay kit will be built and optimized
for your requirements.
*Antibody content may be different between Procarta and ProcartaPlex
Visit www.eBioscience.com/multiplex for information regarding characteristic assay
details and to find a complete listing of multiplex panels and individual analytes.
1
2
Technology Overview
xMAP® Technology
The xMAP technology utilizes two bead types—magnetic and polystyrene. The magnetic
MagplexTM bead is a superparamagnetic 6.5 micron microsphere with a magnetic core and
polystyrene surface. The polystyrene MicroplexTM bead is a 5.6 micron polystyrene microsphere.
Both bead types are internally dyed with precise proportions of red and infrared fluorophores.
The differing proportions of the red and infrared fluorophores result in 100 unique spectral
signature microspheres which are identified by the Luminex® xMAP detection systems. The
conjugation of a distinct monoclonal antibody to a distinct bead allows for analysis of multiple
analytes in a single well.
The polystyrene bead requires expensive, and sometimes difficult to use filter plates. This bead
type also makes automating the assay more difficult. The magnetic bead provides significant
advantages over the polystyrene bead because it allows for greater ease and flexibility. This
ease is realized in the ability to use a flat-bottom plate with either a hand-held magnetic plate
washer (cat. no. EPX-55555-000) or an automated magnetic plate washer. In addition, a filter
plate/vacuum manifold system, commonly used with polystyrene beads, can also be used with
magnetic beads.
6.5 Microns
Bead Set 26
The bead is
impregnated
with the dye
mixture
Bead Set 21
10
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iqu
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red
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on
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Luminex® Instrumentation
Luminex instrumentation is comprised of three instrument models—MAGPIX, LX200 and
FlexMAP 3D. These instruments are based on either the principles of quantitative fluorescent
microscopy or fluorescent flow cytometry. The multiplex quantitation capability of an
assay is dependent upon instrument model, and ranges from 50 analytes with use of the
MAGPIX, to 500 analytes with the FlexMAP 3D. Used in combination with ProcartaPlex, the
Luminex platform delivers fast and cost-effective immunoassay profiling analysis.
Visit www.ebioscience.com/multiplex for more information about xMAP Technology,
Luminex Instruments, and ProcartaPlex Assays
2
ProcartaPlex™ Immunoassay Workflow
Figure 1
Capture Target Analytes

IL-6
IL-8
IFNγ
Capture Beads
IL-6
Figure
IL-62
IL-6
IL-6
IL-8
IFNγ
added to prepared sample. The
unique, differentially dyed capture
beads are coated with target-specific
capture antibodies. This allows for
the unique identification of
the analyte.
IL-6
IL-8
IFNγ
Incubate 1-2 hours and wash
IL-6
IL-8
IFNγ
IL-6
IL-8
IFNγ
Analyte
IL-8
IFNγ
IL-8
IFNγ
IL-8
IFNγ
Incubation
/ Wash
IL-6
IL-8
IL-6
IL-8
Detect Captured Analyte

IL-6
IFNγ
Captured bead/analytes are
incubated with biotinylated analytespecific detection antibody to form
an immuno (sandwich) complex.
IFNγ
Detection Antibody
Analyte-specific capture beads are
IL-8
IFNγ
Immuno (sandwich) Complex
IL-6
IL-8
IFNγ
Incubate 30 min and wash
Figure 3
IL-6
IL-8
IFNγ
IL-6
IL-8
IFNγ

Incubation
/ Wash
IL-6
IL-8
IL-8
IL-8
IFNγ
For analyte quantitation, the immuno
(sandwich) complex is incubated with
a fluorescent detection label (SA-PE)
IFNγ
IL-6
IL-6
Label Detection

The sample is then washed to remove
excess SA-PE
IFNγ
SA-PE
IL-6
IL-8
IFNγ

Multiplex sample is ready for
quantitation
Incubate 30 min and wash
Figure 4
IL-6 (25)
100000
IL-10/CSIF (28)
IL-12/IL-23p40 (33)
MFI
Read & Analyze
IL-5 (21)
10000
MIG/CXCL9 (57)
1000

100/200, Magpix, or
Bio-Plex instrument
100

10
Read with Luminex
Analyze results
1
1
10
100
1000
10000
100000
Concentration (pg/mL)
Secreted proteins simultaneously measured using
ProcartaPlex Cytokine Assay.
3
Product Focus: ProcartaPlex
3
Human Th1/Th2 Panels
ProcartaPlex Human Th1/
Th2 Cytokine Panel
11 plex
EPX110-10810-901
GM-CSF
IL-6
IFNγ
IL-12p70
IL-1ß
IL-13
IL-2
IL-18
IL-4
TNFα
IL-5
TM
Th1/Th2 T Helper Cell Cytokine Panels
Th1 and Th2 lymphocytes are critical mediators of cellular and humoral (antibodymediated) immune responses, respectively. Th1 cells play an important role in host
defense systems for intracellular microbial agents and viruses. Th1 cell promoting factors
include IFNγ and IL-12(p70) and are the primary source for the inflammatory cytokines
lFNγ, IL-2, and TNFβ (LTα) that stimulate other defense cells for the destruction of bacterial
pathogens. These cytokines also help foster the development of cytotoxic lymphocytes
that are responsible for the cell-mediated immune response against viruses and tumor
cells. Alternatively, Th2 cells mediate the humoral response by producing various cytokines
such as IL-4, IL-5, IL-6, IL-9, IL-13 and IL-17E (IL-25). Some of these cytokines counteract the
Th1 responses. Functionally, Th2 cells stimulate and recruit specialized subsets of immune
ProcartaPlex Human
Essential Th1/Th2
Cytokine Panel 6 plex
EPX060-10009-901
IFNγ
cells to the site of infection or in response to allergens or toxins.
Due to the central role of Th1 cells in the immune system, over activation or misdirected
activation also makes them key players in Th1-dominant autoimmune diseases such as multiple
sclerosis, type-1 diabetes, rheumatoid arthritis and delayed-type hypersensitivity responses.
IL-4
IL-5
Over activation of Th2 cells appears to be responsible for the exacerbation of allergies
IL-6
(Type-1, immediate hypersensitivity reactions), autoimmune reactions such as chronic
IL-12p70
graft-versus host disease, progressive systemic sclerosis and systemic lupus erythematosus.
TNFα
Additionally, Th2 response is also implicated in the development of asthma and other
allergic inflammatory diseases
Mouse Th1/Th2 Panels
ProcartaPlex Mouse Th1/
Th2 Cytokine Panel
11 plex
EPX110-20820-901
IL-12p70
IL-1ß
IL-13
IL-2
IL-18
IL-4
TNFα
TH0
IL-27 (early)
IL-12p70 (+IL-15) (m)
RANKL
L)
ANK
IL-5
TCR
R
54 (
IL-6
IFNγ
CD2
GM-CSF
IL-2
IL-4
IL-15 (h)
IL-17E/IL-25
IL-31
IL-33
Tim
3
IL-4
IL-5
IL-6
IL-12p70
TNFα
4
IL-12RB2
IL-18R
CD4
ICO
S
6
CD2
4
IFNγ
Tim1
TH1
NOTCH3
CD9
EPX060-20831-901
TCR
FasL
CD195
ProcartaPlex Mouse
Essential Th1/Th2
Cytokine Panel 6 plex
IL-2
IFNγ
TNFα
TNFß
T-bet
RUNX3
STAT1
STAT4
CD4
TH2
NOTCH1
CH2
NOT
CXC
R4
RB
7
L-1
I
CD30
CCR8
Gata3
STAT6
IL-1
IL-3
IL-4
IL-5
IL-6
IL-10
IL-13
IL-17E/IL-25
IL-31
GM-CSF
ProcartaPlex Multiplex Immunoassays provide scalability with constancy of data. Whether your research drives the
demand for a large 34 plex, a simple 10 plex, or possibly a single target ELISA, you can be confident the ProcartaPlex
assays deliver consistent data.
Multiplex with Elisa Correlation and Sensitivity
Multiplex with ELISA Correlation and Sensitivity
Human
IL-1β ProcartaPlex [pg/ml]
Human IL-1β ProcartaPlex [pg/ml]
250.0
200.0
R2 = 0.98654
150.0
100.0
50.0
0.0
0.0
50.0
100.0
150.0
200.0
250.0
300.0
Human
Platinum
ELISA[pg/ml]
[pg/ml]
Human
IL-1βIL-1β
Platinum
ELISA
Figure 1: Regression analysis compares IL-1β quantitation in either LPS or PMA/Ionomycin stimulated PBMC cell
culture supernatants diluted in normal human serum samples (various concentrations) using a ProcartaPlex Multiplex
Immunoassay and Platinum ELISA, a traditional sandwich ELISA.
Scalability with Consistency
14.0
12.0
Scalability with
with Consistency
Scalability
Consistency
10.0
14.0
8.0
12.0
10.0
6.0
8.0
4.0
6.0
2.0
4.0
18
e
17
pl
e
m
e
pl
ru
m
Sa
m
pl
Sa
m
ru
IL-6 34 plex
IL-6 34 plex
Se
e
er m
2
um
S
SSee Samam
rruum pl pl
mS e 1 e
3
aSm
Se
Se rum apmle
ru S p2le
Se m am
4
ru S pl
Se m Sa ame 3
Seru m pl
ru m ple e
m
4 5
SSe SaSmam
err
p
uum
mS lep5le
6
aSm
Se
r
Se um apmle
ru Sa p6le
Se m m
7
ru S ple
Se m S am 7
a
Seru m p
ru m pl le
e
m
8 8
SS
SSeer amapm
p
l
u
e
rum
mSa 9le 9
Se
Smap
ru
Se m mle 1
pl0
Seru Sam
e
ru m p
10
m S le
Se Sa am 11
m
Ser
ruum plepl
m
12e 1
SaS
1
Se
Se ru mapml
epl
rum
13e
S
m am
12
Se
Sp
Se rum amle 1
r S pl4
Se um am
p e
ru
m S le 1 13
SSe Samam 5
err
p p
uum
mS le 1le 1
6 4
aSm
S
Seeru apmle
m
p
ru S
1
m am l7e 1
5
Saple
Se
m 18
pl
S ru
m
Se
m
m
Sa
m
ru
Se
ru
Se
Sa
pl
e
1
0.0
16
2.0
0.0
IL-6 Th1/Th2 11 plex
IL-6 Th1/Th2 11 plex
Figure 2: To illustrate ProcartaPlex scalability 18 diseased human serum samples were run in parallel using both the ProcartaPlex
Human Cytokine & Chemokine Panel 1A (34 plex) and the ProcartaPlex Human Th1/Th2 Cytokine Panel (11 plex). The data in
this experiment demonstrates the consistency achieved in both a very large and smaller scale multiplex panel.
5
Tfh/Th9/Th17/Th22/TReg T Helper Cell
Cytokine Panels
In addition to the Th1/Th2 cytokine expression profiles, there are many other CD4+ T Helper
cell types that also have distinctive differentiation and secretion cytokine profiles. Each of
these different cell types have very important roles in the body’s immune response. Th17 and
TReg cell types have gained the greatest amount of interest over the last decade with Tfh now
gaining considerable interest. Other T helper cells, such as Th9 and Th22, also exist.
Th17 cells act as a bridge between adaptive and innate immunity. Cell differentiation
is driven by TGFβ and IL-6 in vitro, with IL-1β and IL-23 also being necessary in vivo for
Th17 development. Th17 cells require IL-23 for expansion and stabilization while the
cytokines IFNγ, IL-27 and IL-4 inhibit differentiation. Activated Th17 cells produce the
cytokines IL-17A, IL-17F, IL-17AF, IL-21, IL-22, IL-26 (human), GM-CSF, MIP-3α and TNFα.
Functionally, Th17 cells play a key role in host defense against extracellular microbes and in
autoimmune disease. Its inflammatory responses have been implicated in a broad array of
inflammatory and autoimmune diseases.
Regulatory T (TReg) cells function to maintain self-tolerance and immune homeostasis
by suppressing the activation, proliferation and effector functions of various immune
cells. One mechanism for this involves the secretion of IL-10 which inhibits effector T cell
responses. TReg cells also secrete IL-35 and TGFβ that induces conventional CD4+ T cells
to differentiate into TReg cells, thereby skewing the ratio of TReg to T helper cells during
an immune response. Understanding the mechanisms by which TReg cells exert their
suppressive function has broad implications for drug development strategies aimed at
treating cancer, diabetes and other autoimmune diseases.
T follicular helper (Tfh) cells are essential in the development of antigen-specific
effector and memory B cell responses. They regulate humoral immunity and play a role
in the development of long-term antibody responses. They require IL-6, IL-21 and B cell
interaction for complete development. Upon antigen-specific stimulation, Tfh cells release
IL-4, IL-10, IFNγ and IL-21 to stimulate mature B cells into forming germinal centers and
undergoing antibody class-switching.
Th9 cells are characterized by the secretion of IL-9. Th9 cells differentiate with the
combination of TGFβ and IL-4, while the cytokines IL-1β, IL-6, IL-10, IL-21, IFNα and
IFNβ enhance IL-9 expression of cultured Th9 cells. Conversely, IFNγ and IL-27 inhibit its
production in human Th9 cells making it possible that Th1 response may suppress Th9 cell
differentiation. Th9 cells are also known to be capable of inducing tissue inflammation,
Experimental Allergic Encephalomyelitis (EAE), and play a role in allergic asthma.
Th22 cells are a subset dedicated to the production of IL-22, an IL-10 family member.
Maturation of Th22 cells requires TNFα and IL-6. Th22 cells are characterized by the
expression of IL-22, IL-13 and other factors including FGF isoforms involved in tissue
remodeling. Th22 cells appear to provide a protective role in regulating wound repair and
healing in the skin, gut and lungs.
6
TH0
TGFß
IL-4
CD
CD4
3
IL-1ß
TGFß+IL-6
IL-21
IL-23
CCR9
FR4 (m)
GARP
-4
CTLA
R/A
TCR
TNFα
IL-6
GIT
Foxp3
AhR
STAT5
(activ
ated)
ITR
12
CD
4
TCR
RB
1
IL-1
R1
ICOS
TGFß
IL-10
IL-35
R
IL-
IL-6
α1
R
IL-13
TH17
IL-21R
TFH
IL-21R
IL-23R
CD84
CD132
TCR
CXC
R5
CD
4
CCR1
0
30
61
IL-17A
IL-17F
IL-17AF
IL-21
IL-22
IL-24
IL-26
MIP-3α (CCL20)
GM-CSF
TNFα
gp1
4
CD
CCR6
BCL-6
STAT3
CD1
RUNX1
Batf
IRF4
RORγt
RORα
STAT3
AhR
c-maf
CD101
TReg
CXCL13
CCR4
IL-6
IL-21
B Cell Interaction
IL-2
IL-4
IL-27
IFNγ
IL-9
73
CD
CXCR
TCR
TH9
39
CD4
CD2
5
IL-2
TGFβ
R
TH22
PDGF
IL-2
IL-4
IL-10
IL-12
IL-21
IFNγ
TCR
IL-13
IL-22
FGF
TNFα
Additional Human T Helper Cell Panels
ProcartaPlex Human Th1/
Th2/Th9/Th17/Th22/Tfh/
Treg Cytokine Panel
(18 plex)
ProcartaPlex Human Th9/
Th22/Th17/Treg
Cytokine Panel
(7 plex)
EPX180-12165-901
GM-CSF
IL-12p70
IFNγ
IL-13
IL-1ß
IL-17A
IL-2
IL-18
IL-4
IL-21
IL-5
IL-22
IL-6
IL-23
IL-9
IL-27
IL-10
TNFα
EPX070-10817-901
IL-9
IL-10
IL-17A
IL-21
IL-22
IL-23
IL-27
Additional Mouse T Helper Cell Panels
ProcartaPlex Mouse Th1/
Th2/Th9/Th17/Th22/Treg
Cytokine Panel
(17 plex)
ProcartaPlex Mouse Th9/
Th22/Th17/Treg
Cytokine Panel
(6 plex)
EPX170-26087-901
EPX060-20822-901
GM-CSF
IFNγ
IL-1ß
IL-2
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12p70
IL-13
IL-17A
IL-18
IL-22
IL-23
IL-27
TNFα
IL-9
IL-10
IL-17A
IL-22
IL-23
IL-27
7
4
Human ProcartaPlex™ Multiplex Panels & Simplex Kits
Magnetic Bead Panels
ProcartaPlex Human Cytokine/
Chemokine/Growth Factor
Panel 1 (45 plex)
ProcartaPlex Human Cytokine
& Chemokine Panel 1A
(34 plex)
COMING SOON!
BDNF
CD40L/TNFSF5
EGF
Eotaxin/CCL11
FGF-2
GM-CSF
GROα/CXCL1
HGF
IFNα
IFNγ
IL-1RA
IL-1α
IL-1ß
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8/CXCL8
IL-9
IL-10
IL-12p70
IL-13
IL-15
IL-17A
IL-18
IL-21
IL-22
IL-23
IL-27
IL-31
IP-10/CXCL10
LIF
MCP-1/CCL2
MIP-1α/CCL3
MIP-1β/CCL4
βNGF
PDGF-BB
PLGF
RANTES/CCL5
SCF
SDF1α/CXCL12
TNFα
TNFß/LTA
VEGF-A
ProcartaPlex Human Th1/Th2/Th9/
Th17/Th22/Tfh/Treg
Cytokine Panel (18 plex)
EPX180-12165-901
GM-CSF
IFNγ
IL-1ß
IL-2
IL-4
IL-5
IL-6
IL-9
IL-10
8
IL-12p70
IL-13
IL-17A
IL-18
IL-21
IL-22
IL-23
IL-27
TNFα
ProcartaPlex Human
Cytokine Panel 1B
(25 plex)
EPX340-12167-901
Eotaxin/CCL11
GM-CSF
GROα/CXCL1
IFNα
IFNγ
IL-1α
IL-1ß
IL-1RA
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8/CXCL8
IL-9
IL-10
IL-12p70
IL-13
IL-15
IL-17A
IL-18
IL-21
IL-22
IL-23
IL-27
IL-31
IP-10/CXCL10
MCP-1/CCL2
MIP-1α/CCL3
MIP-1β/CCL4
RANTES/CCL5
SDF1α/CXCL12
TNFα
TNFß/LTA
EPX250-12166-901
GM-CSF
IL-12p70
IFNα
IL-13
IFNγ
IL-15
IL-1α
IL-17A
IL-1ß
IL-18
IL-1RA
IL-21
IL-2
IL-22
IL-4
IL-23
IL-5
IL-27
IL-6
IL-31
IL-7
TNFα
IL-9
TNFß/LTA
IL-10
ProcartaPlex Human
Th1/Th2 Cytokine Panel
(11 plex)
ProcartaPlex Human Essential
Th1/Th2 Cytokine Panel
(6 plex)
EPX110-10810-901
EPX060-10009-901
GM-CSF
IFNγ
IL-1ß
IL-2
IL-4
IL-5
IL-6
IL-12p70
IL-13
IL-18
TNFα
IFNγ
IL-4
IL-5
IL-6
IL-12p70
TNFα
ProcartaPlex Human
Th9/Th22/Th17/Treg Cytokine Panel
(7 plex)
ProcartaPlex Human
Cytokine Panel IC
(7 plex)
ProcartaPlex Human
Chemokine Panel I
(8 plex)
EPX070-10817-901
EPX070-10010-901
EPX080-10007-901
Eotaxin/CCL11
IFNα
IL-1α
IL-1RA
IL-7
IL-15
IL-31
TNFß
IL-9
IL-10
IL-17A
IL-21
IL-22
IL-23
IL-27
GROα/CXCL1
IL-8/CXCL8
IP-10/CXCL10
MCP-1/CCL2
MIP-1α/CCL3
MIP-1β/CCL4
SDF1α/CXCL12
Human SimplexTM Bead Set Reagent Kits
Add Simplex bead sets to your ProcartaPlex panel to fine tune your cytokine analysis, or combine
multiple Simplexes and run using the ProcartaPlex Basic kit.
Human ProcartaPlex Simplex – Bead Set Reagent Kits
Analyte
Cat. No.
Analyte
Cat. No.
Analyte
Cat. No.
Analyte
Cat. No.
CD40L
ENA-78
Eotaxin
Eotaxin-3
Fractalkine
GM-CSF
GROα
IFNα
IFNβ
IFNγ
IL-1α
IL-1β
EPX010-10239-901
EPX010-12119-901
EPX010-12120-901
EPX010-12140-901
EPX010-12121-901
EPX010-10283-901
EPX010-12122-901
EPX010-10216-901
EPX010-12088-901
EPX010-10228-901
EPX010-10243-901
EPX010-10224-901
IL-1RA
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8
IL-9
IL-10
IL-12p40
IL-12p70
IL-13
EPX010-12080-901
EPX010-10221-901
EPX010-10225-901
EPX010-10278-901
EPX010-10213-901
EPX010-10237-901
EPX010-10204-901
EPX010-12081-901
EPX010-10215-901
EPX010-12090-901
EPX010-10238-901
EPX010-10231-901
IL-15
IL-17A
IL-18
IL-22
IL-23p19
IL-27
IL-31
IP-10
LIF
M-CSF
MCP-1
MIP-1α
EPX010-12089-901
EPX010-12017-901
EPX010-10267-901
EPX010-12047-901
EPX010-12023-901
EPX010-12085-901
EPX010-10241-901
EPX010-10284-901
EPX010-10242-901
EPX010-12126-901
EPX010-10281-901
EPX010-12029-901
MIP-1β
MIP-3α
RANTES
SAA
SCF
SDF-1α
sFas-L
TGFα
TNFα
TNFβ
TRAIL
EPX010-12030-901
EPX010-12128-901
EPX010-10287-901
EPX010-12136-901
EPX010-12137-901
EPX010-12138-901
EPX010-10260-901
EPX010-12139-901
EPX010-10223-901
EPX010-10202-901
EPX010-12004-901
Human Basic Kit
Description
Catalog Number
Includes all non-target specific reagents – assay diluent, wash buffers,
SA-PE and plates – needed to perform the ProcartaPlex Simplex assays
EPX010-10420-901
9
5
Mouse ProcartaPlex™ Multiplex Panels & Simplex Kits
Magnetic Bead Panels
ProcartaPlex Mouse Cytokine &
Chemokine Panel 1 (26 plex)
EPX260-26088-901
Eotaxin/CCL11
GM-CSF
GROα/KC/CXCL1
IFNγ
IL-1ß
IL-2
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12p70
IL-13
IL-17A
IL-18
IL-22
IL-23
IL-27
IP-10/CXCL10
MCP-1/CCL2
MCP-3/CCL7
MIP-1α/CCL3
MIP-1β/CCL4
MIP-2/CCL8
RANTES/CCL5
TNFα
ProcartaPlex Mouse Th1/Th2/Th9/
Th17/Th22/Treg Cytokine Panel
(17 plex)
ProcartaPlex Mouse
Th1/Th2 Cytokine Panel
(11 plex)
EPX170-26087-901
EPX110-20820-901
GM-CSF
IFNγ
IL-1ß
IL-2
IL-4
IL-5
IL-6
IL-9
IL-10
ProcartaPlex Mouse
Essential Th1/Th2 Cytokine
Panel (6 plex)
ProcartaPlex Mouse
Th9/Th22/Th17/Treg
Cytokine Panel (6 plex)
EPX060-20831-901
EPX060-20822-901
IFNγ
IL-4
IL-5
IL-6
IL-12p70
TNFα
IL-9
IL-10
IL-17A
IL-22
IL-23
IL-27
IL-12p70
IL-13
IL-17A
IL-18
IL-22
IL-23
IL-27
TNFα
GM-CSF
IFNγ
IL-1ß
IL-2
IL-4
IL-5
IL-6
IL-12p70
IL-13
IL-18
TNFα
ProcartaPlex Mouse
Chemokine Panel 1
(9 plex)
Mouse Isotyping
(7 Plex)
EPX090-20821-901
EPX070-20815-901
GROα/KC/CXCL1
IP-10
MCP-1
MCP-3
MIP-1α
MIP-1β
MIP-2
RANTES
Eotaxin
IgG1
IgG2a
IgG2b
IgG3
IgA
IgE
IgM
Mouse SimplexTM Bead Set Reagent Kits
Add Simplex bead sets to your ProcartaPlex panel to fine tune your cytokine analysis,
or combine multiple Simplex sets and run using the ProcartaPlex Basic kit.
Mouse ProcartaPlex Simplex bead set reagent kits
Analyte
Cat. No.
Analyte Cat. No.
Analyte
Cat. No.
Analyte
Cat. No.
Eotaxin/
CCL11
EPX010-26008-901
IL-4
EPX010-20613-901
IL-21
EPX010-26021-901
M-CSF
EPX010-26039-901
G-CSF/
CSF-3
EPX010-26034-901
IL-5
EPX010-20610-901
IL-22
EPX010-26022-901
MIP-1α/
CCL3
EPX010-26013-901
GM-CSF
EPX010-20612-901
IL-6
EPX010-20603-901
IL-23p19
EPX010-26017-901
MIP-1β/
CCL4
EPX010-26014-901
GROα
EPX010-26031-901
IL-9
EPX010-26041-901
IL-27
EPX010-26024-901
MIP-2/
CXCL2
EPX010-26032-901
IFNγ
EPX010-20606-901
IL-10
EPX010-20614-901
IP-10
EPX010-26018-901
RANKL
EPX010-26037-901
EPX010-26030-901
RANTES/
CCL5
EPX010-26009-901
IL-1β
10
EPX010-26002-901
IL-12p70 EPX010-26004-901
LAP (TGFβ)
IL-2
EPX010-20601-901
IL-13
EPX010-26015-901
MCP-1/CCL2 EPX010-26005-901
TNFα
EPX010-20607-901
IL-3
EPX010-26035-901
IL-17A
EPX010-26001-901
MCP-3
VEGF-A
EPX010-20619-901
EPX010-26006-901
Procarta® Mix&Match
Build custom-blended and optimized multiplex panels. Choose the appropriate analytes from each of the Procarta Mix&Match groups
listed below, then select your sample type and instrument. Your assay kit will be custom built and optimized to these requirements.
Human Mix&Match Analytes
Cytokine Group 1 (54 plex)
βNGF/NGFB
sCD40L (CD40 Ligand)
EGF
ENA-78 (CXCL5)
Eotaxin (CCL11)
FGF basic (FGF-2)
Fractalkine (CX3CL1)
G-CSF (CSF-3)
GM-CSF
GROα (CXCL1)
HGF
IFNα2
IFNβ
IFNγ
IL-1
IL-1β
IL-1RA
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8 (CXCL8)
IL-9
IL-10 (CSIF)
IL-12/IL-23 p40
IL-12 p70
IL-13
IL-15
IL-16
IL-17A (IL-17)
IL-17F
IL-20
IP-10 (CXCL10)
I-TAC (CXCL11)
Leptin
MCP-1 (CCL2)
MCP-3 (CCL7)
M-CSF (CSF-1)
MIG (CXCL9)
MIP-1α (CCL3)
MIP-1β (CCL4)
MIP-3α (CCL20)
PAI-1 (Serpin E1)
PDGF-BB
RANTES (CCL5)
Resistin
SAA (serum amyloid A1)
TGFα
TGFβ1*
TNFα
TNFβ (TNFSF1, LTA)
TRAIL (TNFSF10)
VEGF-A
Cytokine Group 2 (36 plex)
Adiponectin
BDNF
CXCL13 ( BCA-1, BLC)
C-TACK (CCL27)
E-selectin (CD62E)
Eotaxin-2 (CCL24)
Eotaxin-3 (CCL26, MIP-4 )
Fas (TNFRSF6)
Fas Ligand
Granzyme B
ICAM-1 (CD54)
IFNω
IL- 2RA (CD25)
IL- 3
IL-21
IL-22
IL-23 p19
IL-27
IL-29
MMP Group 1 (5 plex)
MMP-1
MMP-7
MMP-8
MMP-12
MMP-13
IL-31
LIF
MIF
MPO (Myeloperoxidase)
OPG (Osteoprotegerin)
OSM/Oncostatin M
PIGF-1
RANKL (TNFSF11)
SAP (Pentraxin2, APCS)
SCF (KIT Ligand)
SLPI
SCGFβ (CLEC11A)
SDF-1 (CXCL12)
Survivin (BIRC5)
TNFRI (TNFRSF1A)
TNFRII (TNFRSF1B)
TPO (Thrombopoietin)
VCAM-1 (CD106)
BAFF (BlyS, CD257)
TSLP
MMP Group 2 (3 plex)
MMP-2
MMP-3
MMP-9
Mouse Mix&Match Analytes
Cytokine Group 1 (37 plex)
Adiponectin
BTC (Betacellulin)
Eotaxin (CCL11)
G-CSF (CSF-3)
GM-CSF (CSF-2)
GROα (KC/CXCL1)
IFNγ
IL-1α
IL-1β
IL-2
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10 (CSIF)
IL-12/IL-23 p40
IL-12 p70
IL-13
IL-15
IL-17A
IL-21
IL-23 p19
IP-10 (CXCL10)
Leptin
LIF
CXCL5 (LIX/GCP2)
MCP-1 (CCL2)
MCP-3 (MARC/CCL7)
M-CSF (CSF-1)
MIP-1α (CCL3)
MIP-2/GRO β/CINC3/CXCL2
RANTES (CCL5)
RANKL (TNFSF11)
TGFβ1*
TNFα
VEGF-A
Cytokine Group 2 (7 plex)
Single Plex Assays
BlyS/BAFF/CD257/TNFSF13B
IL-7
IL-17F
MIP-1β (CCL4)
sIL-2RA (CD25)
sIL-7RA (CD127)
TSLP
TGFβ1*
CRP/C-Reactive Protein
Antibody Isotyping Group
IFN alpha/beta
IgG1
IgG2a
IgG2b
IgG3
IgA
IgM
*Note: Please inquire for the multiplexing of analytes between different groups.
Visit www.ebioscience.com/product-line/procartaplex/mixandmatch for more information.
IFNα
IFNβ
11
Non-Human Primate Mix&Match Analytes
Cytokine Group 1 (45 plex)
Adiponectin
βNGF/NGFB
CXCL13 (BCA-1, BLC)
CD40L (CD40 ligand, TNFSF5)
Eotaxin (CCL11)
FGF Basic
G-CSF (CSF-3)
GM-CSF (CSF-2)
Granzyme B
ICAM-1 (CD54)
IFNγ
IL-1β
IL-2
IL-4
IL-5
Single Plex Assays
IL-6
IL-8 (CXCL8)
IL-10
IL-12/IL-23 p40
IL-12 p70
IL-13
IL-15
IL-17A (IL-17)
IL-17F
IL-1RA
IL-23 p19
IP-10 (CXCL10)
I-TAC (CXCL11)
MCP-1 (CCL2)
MCP-3 (CCL7)
MIF
MIG (CXCL9)
MIP-1α (CCL3)
MIP-1β (CCL4)
PAI-1 (Serpin E1)
PDGF-BB
RANTES/CCL5
Resistin/ADSF
TGFα
TGFβ1*
TNFα
TNFβ (LTA)
TRAIL
VCAM-1 (CD106)
VEGF-A
TGFβ1*
Rat Mix&Match Analytes
Cytokine Group 1 (30 plex)
βNGF/NGFB
Eotaxin/CCL11
G-CSF/CSF-3
GM-CSF/CSF-2
GRO /KC/CINC1/ CXCL1
ICAM-1/CD54
IFNγ
IL-1 /IL-1F1
IL-1 β/IL-1F2
IL-2
IL-4
IL-5
IL-6
IL-10/CSIF
IL-12/IL-23 p40
IL-12 p70
IL-13
IL-17A/CTLA-8
IP-10/CXCL10
Leptin/LEP
MCP-1/JE/CCL2
MCP-3/MARC/CCL7
MIP-1 /CCL3
MIP-2/GRO β/CINC3/CXCL2
RANTES/CCL5
RANKL/TNFSF11
TGFβ1*
TNF
VCAM-1/CD106
VEGF-A
Single Plex Assays
TGFβ1*
CRP (C-Reactive Protein)
Canine Mix&Match Analytes
Cytokine Group 2 (7 plex)
βNGF/NGFB
IFNγ
IL-2
IL-6
IL-8 (CXCL8)
IL-10
IL-12/IL-23 p40
MCP-1 (CCL2)
SCF (c-kit Ligand)
TGFβ1*
TNF
VEGF-A
Single Plex Assays
TGFβ1*
CRP (C-Reactive Protein)
Porcine Mix&Match Analytes
Cytokine Group 1 (10 plex)
IFNalpha
IFNγ
IL-1 β/IL-1F2
IL-4
IL-6
12
L-8/CXCL8
IL-10/CSIF
IL-12/IL-23 p40
TGFβ1*
TNFalpha
Single Plex Assays
TGFβ1*
* It is recommend to run TGFβ1 as a single plex assay because the sample should be acid-treated for proper detection
of the bioactive form. Not all products available in all regions. Please contact customer service for more information.
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