trygve edvardsen

MARPLAN: European integration of
marine microplankton research
Department of Biology, University of Oslo (UIO),
NORWAY
Bente Edvardsen and Wenche Eikrem
Kick-off meeting Napoli September 23, 2005
Department of Biology
6 Research Programs
1. CEES (Center for
evolutionary and
ecological synthesis)
2. Experimental behavioural
and population biology
3. Molecular ecology and
biosystematics
4. Marine biodiversity
5. Plankton biology
6. Toxicology and
ecophysiology
marine biology marked in blue
Program for Plankton Biology
MARPLAN participants
6 Professors and Associate
Professors
3 Researchers and Post
doctors
Wenche
Bente
Jahn
Tom
11 PhD students
10 Master students
4 Technical staff
Ingvild Riisberg
Kjetil Røberg
Søren Larsen
Technical infrastructure
•Flow cytometers
•Laser scanner cytometer
(Compucyte iCys )
Electron Microscopy
Laboratory (TEM, SEM, CM)
DNA-sequencing
service (2x ABI 3730)
Labs: plankton, chemistry, molecular
biology, particle analysis
F/F Trygve Braarud
Culture rooms with
culture collection
The Biological
Station in Drøbak
Research activities by MARPLAN
participants
A Phylogeny, taxonomy and biology
(JT, WE, BE, IR, KR)
B Biodiversity and genetic diversity
(JT, WE, BE, IR, KR)
C Harmful algae and toxin production
(BE, IR, WE, JH)
D Plankton dynamics and ecological stoichiometry
(TA, SL, BE)
E Planktonic food webs
(TA, SL)
Organisms - harmful algae
Chrysochromulina
Prymnesium
Planktothrix
bloom
dynamics
life cycle
toxin production
phylogeny
Dinophysis
Verrucophora
genetic variation
taxonomy
gene expression
probe development
Haptophyte SSU rDNA phylogeny
Pavlova aff. salina
Pavlova gyrans
Pavlova CCMP1416
99/100
Pavlova CCMP 1394
Phaeocystis sp. 1
100/100
OLI51004
Phaeocystis globosa
99/
100/100
Phaeocystis pouchetii
100
96/75
Phaeocystis antarctica
Phaeocystis sp. 2
100/100 OLI51059
OLI51033, OLI51056
Chrysochromulina hirta
99/67
Chrysochromulina kappa
97/100
Chrysochromulina polylepis
66/Prymnesium calathiferum
98/97
Prymnesium parvum
-/60
Prymnesium patelliferum
100/100 Prymnesium nemamethecum
75/86
Imantonia rotunda
100/100
Chrysochromulina acantha
100/100
Chrysochromulina throndsenii
Chrysochromulina scutellum
73/99
Chrysochromulina campanulifera
76/57
58/89
OLI16108
79/89
OLI26017
OLI51102
79/98
66/98
OLI16029
100/100
Cruciplacolithus neohelis
Coccolithus pelagicus
98/98
Reticulosphaera japonensis
Pleurochrysis sp. CCMP 875
99/99 100/100
Pleurochrysis elongata
Pleurochrysis carterae
93/74
99/99
CCMP 300
100/100
Isochrysis galbana
Emiliania huxleyi/Gephyrocapsa oceanica
65/89
CCMP 625
100/100
OLI51050
CLADE E
OLI26041
100/100
100/100
2%
87/57
100/100
100/100 OLI16010
76/65
60/60
OLI51080
OLI26047
OLI51076
}
CLADE A
}
}
CLADE B1
CLADE B2
}
CLADE C
}
}
CLADE D
Crypthecodinium
Fucus
Edvardsen et al. 2000
Verrucophora fascima (Dictyochophyceae)
sp. et gen. nov.
fl
mu
10 µm
ultrastructure
thin section of
Chrysochromulina leadbeateri
Photo: Wenche Eikrem
Dinoflagellate SSU
rDNA-tree with
probes for
Dinophysis
* *
**
**
(Modified from Edvardsen et al. 2003)
Pico and nanoplankton, identification and description of new species
C
h
l
o
r
o
p
h
y
t
a
Mamiellales
Flagellates of sandy shores
Biodiversity (microscopy)
Community studies in time and space (DGGE)
Statistical niche modeling
z Generalized Additive Models (GAM)
z Cross-validated thin-plate spline models
z R package mgcv (Simon Wood)
z Phytoplankton data for demonstration
z Finnish Coastal monitoring (SYKE)
z 1304 samples from 60 stations (1970-2001)
z Aggregated to 147 station-year averages
z 462 counting units, aggregated to 118 genera
z Presence = > 0.01% of average biomass
z Salinity and total N as explanatory variables