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MSC Spring Brochure - Medical Supply Company
MSC Promotion Q1 2016 Please reference MSCQ12016€ to avail of offers Valid until 15/04/2016 NEW JetSeq DNA Library Prep kits from Bioline JetSe DNA Prepa End-repair, A-tailing and ligation all in 1 Tube! • 3-4-2 on Bioline SensiFast QPCR mastermixes • New Mirus TransIT-PRO transfection reagent for CHO cells • Biolegend Mojosort magnetic cell separation system • New Glycan, Lectin and other pathway arrays from Raybiotech • Low input: end-repair, A-tailing and ligation combined in the same tube, thereby eliminating cleanup steps and improving sample yield The JetSeq™DNA Library Preparation Kit is designe quality next generation sequencing (NGS) librarie on Illumina® instruments. • New Transomic CRISPR technology The success of next-generation sequencing is partly depe accurate processing of the input DNA. High-quality librar confidence: simpler requires efficient processing during a series of mole •• Improved Clontech Guide-ITDNA Mutation protocol with fewer steps for reduced good recovery during the intermediate purification steps risk of sample loss and offering Detection greater peace-of-mind Kit for CRISPR/Cas9 FASTER LIBRARY CONSTRUCTION • Increased speed: library preparation By combining end repair, A-tailing and ligation in a single Library Preparation • inTakara PCR andtimeQPCR offersKit protocol offers a faster turnaround less than 3 hours for reduced manual effort, thereby enabling complete library construc to results and increased risk sample elimination of purification steps minimizes sample lo • throughput Over 9000’s ELISAsThe thefrom of the JetSeq buffer system ensures high-yield of seq • Highly efficient: reaction buffer pre-optimized to provide maximum ELISAKitassays reaction efficiency and highest DNA Shearing 5’ conversion rates 5’ Overhangs 3’ Overhangs Single Tube 5’ 5’ P Blunt Product 3’ 3’ 3’ Fragmented DNA 3’ 5’ P 5’ P Medical Supply Company, Damastown, Mulhuddart, D. 15 T: 01 822 4222 F: 01• 822 4100 quality: E: [email protected] W: www.medical-supply.ie Improved reliable library preparation from even very challenging samples, providing maximum coverage OH OH HD OH HD P HD P 5’ 5’ 5’ P 5’ End Repair A-Tailing A A P P 5’ JetSeq™ DNA Library Preparation Kit Powering NGS • Low input: end-repair, A-tailing and ligation combined in the same tube, thereby eliminating cleanup steps and improving sample yield • Improved confidence: simpler protocol with fewer steps for reduced risk of sample loss and offering greater peace-of-mind • Increased speed: library preparation in less than 3 hours for reduced time to results and increased risk sample throughput The JetSeq™DNA Library Preparation Kit is designed to generate highquality next generation sequencing (NGS) libraries suitable for sequencing on Illumina® instruments. The success of next-generation sequencing is partly dependent upon the precise and accurate processing of the input DNA. High-quality library preparation from sheared DNA requires efficient processing during a series of molecular biology reactions and good recovery during the intermediate purification steps. FASTER LIBRARY CONSTRUCTION By combining end repair, A-tailing and ligation in a single tube, the JetSeq™ DNA Library Preparation Kit protocol offers a faster turnaround time (Fig. 1), with minimal manual effort, thereby enabling complete library construction in less than 3 hours. The elimination of purification steps minimizes sample loss, while optimization of the of the JetSeq buffer system ensures high-yield of sequence-ready libraries. • Highly efficient: reaction buffer DNA Shearing pre-optimized to provide maximum reaction efficiency and highest conversion rates preparation from even very challenging samples, providing maximum coverage 5’ Overhangs 3’ Overhangs 5’ Single Tube • Improved quality: reliable library 5’ 5’ P 5’ P adaptors, enabling indexing of up to 16 samples, already included in the kit Fragmented DNA 5’ P OH OH HD P 3’ 3’ OH HD • Maximum convenience: sequencing Blunt Product 3’ 3’ HD P 5’ 5’ 5’ P T A 5’ End Repair A-Tailing A A P P 5’ A T Adapter Ligation Cleanup Adapter Extention Cleanup (Optional) Indexing bioline.com/jetseq 2 Code Product BIO-68025 JetSeq DNA Library Preparation Kit Fig. 1 Quality score across all bases The JetSeq DNA Library Preparation Kit protocol incorporates fewer steps. This simpler, shorter protocol reduces both hands-on time and the total time required for preparation of library DNA. Competition Price € €800 Medical Supply Company, Damastown, Mulhuddart, D. 15 T: 01 822 4222 F: 01 822 4100 E: [email protected] W: www.medical-supply.ie strand synthesis over a broad range of RNA template concentrations, making SensiFAST cDNA Synthesis Kit the • Unbiased cDNA synthesis –real-time complete 5’ to®in3’ RNA sequence representation Rapid – cDNA and PCR less than 60 minutes, ideal for high throughput applications ideal partner for synthesis SensiFAST Probe and SYBR real-time PCR kits. • Rapid – cDNA synthesis and real-time PCR in less than 60 minutes, ideal for high throughput applications Code Product Pack Size 2-4-1 Price € cDNA€296 • Reproducible – Unique reverse transcriptase new TransAmp High Sensitivity BIO-65053 SensiFAST cDNA Synthesis Kit withand TransAMP buffer* ™ buffer generate consistent 50 reactionsfirst-strand x2 SensiFAST ™ cDNA Synthesis Kit provides a High Sensitivity • Broad dynamic range – ideal for dilute and low-copy samples *An optimised blend of random and oligo(dT) other components! Just add RNA and SensiFAST RTase and go! rapid and sensitive method for firstand strand SensiFAST ™ cDNA Synthesis Kit provides a cDNA synthesis for use in real-time PCR • Sensitive – real-time analysis from as little as 4pg of starting total RNA rapid and sensitive methodRT-PCR for first strand studies (fig. 1). SensiFAST cDNA Synthesis cDNA synthesis for use in real-time PCR • Unbiased cDNA synthesis – complete 5’ to 3’ RNA sequence representation Kit also displays excellent linearity across studies (fig. 1). SensiFAST cDNA Synthesis a wide range of starting material, revealing Kit also displays excellent linearity Fig.throughput 1b Speed and sensitivity against supplier B Fig. 1ain Speed andthan sensitivity supplierideal Q • Rapid – cDNA synthesis andacross real-time PCR less 60 against minutes, for high applications the same relative representation in your SensiFAST cDNA Synthesis Kit and a kit from supplier B were SensiFAST cDNA Synthesis Kit and a kit from supplier Q a wide range of starting material, revealing also used in a first-strand reactions of total RNA. The results were used in first-strand reactions of total RNA, following the cDNA template, regardless of gene Fig. 1b Speed sensitivity B also Fig. 1a Speedstandard and sensitivity against supplier illustrate that theand SensiFAST cDNAagainst Synthesissupplier Kit (red) is manufacturer’s reaction conditions. A 10-foldQdilution the same relative representation in your Synthesis Kit and athan kit from supplier B were SensiFAST SensiFAST a kit from supplier Q using much fastercDNA and even more sensitive supplier B (blue). series of thecDNA cDNASynthesis was usedKit in and a real-time PCR reaction abundance. Furthermore, SensiFAST also used in a first-strand reactions of total RNA. The results were used in first-strand reactions of total RNA, following the SensiFAST SYBR No-ROX. The results illustrate that the cDNA template, regardless of gene illustrate that the SensiFAST cDNA Synthesis Kit (red) is also manufacturer’s standard reaction conditions. A 10-fold dilution SensiFAST cDNA Synthesis Kit (red) is faster and more sensitive High Sensitivity cDNA Synthesis Kit will produce reliable, much faster and even more sensitive than supplier B (blue). series of the cDNA was used in a real-time PCR reaction using than suppliers Q (blue). abundance.™ Furthermore, SensiFAST SensiFAST SYBR No-ROX. The results illustrate that the SensiFAST cDNAresults, Synthesis Kit provides high performance in both real-timea SensiFAST cDNA Synthesis Kit (red) is faster and more sensitive cDNA Synthesis Kit will produce reliable, rapidconventional and sensitive method for first strand than suppliers Q (blue). and RT-PCR. high performance results, in both real-time cDNA synthesis for use in real-time PCR and conventional RT-PCR. Key features studies (fig. 1). SensiFAST cDNA Synthesis Buy 3 Get 1 Free on Bioline SensiFAST QPCR Reagents ® ® Still using first generation qPCR reagents? Key features Excellent, high sensitivity kits BIO-65053 • Ultra-sensitive for low-copy templates • Fast results on all qPCR instruments BIO-65053 for• Ultra-sensitive use with anytemplates QPCR Synthesis cycler for low-copy • Simple and reproducible, just add primers and template SensiFAST cDNA Kit Kit also displays excellent linearity across a wide range of starting material, revealing the same relative representation in your Fig. 1b Speed and sensitivity against supplier B SensiFAST cDNA Synthesis Kit and a kit from supplier B were also used in a first-strand reactions of total RNA. The results illustrate that the SensiFAST cDNA Synthesis Kit (red) is also much faster and even more sensitive than supplier B (blue). Fig. 1a Speed and sensitivity against supplier Q SensiFAST cDNA Synthesis Kit and a kit from supplier Q were used in first-strand reactions of total RNA, following the manufacturer’s standard reaction conditions. A 10-fold dilution series of the cDNA was used in a real-time PCR reaction using SensiFAST SYBR® No-ROX. The results illustrate that the SensiFAST cDNA Synthesis Kit (red) is faster and more sensitive than suppliers Q (blue). SensiFAST 50 reactionscDNA Synthesis Kit Size BIO-92002 B3G1F SensiFAST SYBR Hi-ROX Kit 4 x 200 rxns 50 reactions Code Product Special Price €282 / £240 BIO-94002 B3G1F SensiFAST SYBR Lo-ROX Kit 4 x 200 rxns BIO-92002 B3G1F SensiFAST SYBR Hi-ROX Kit Special Price €282 / £240 BIO-98002 B3G1F SYBRSYBR No-ROX Kit Kit 4 x 200 rxns BIO-94002 B3G1F SensiFAST SensiFAST Lo-ROX BIO-72001 B3G1F SensiFAST SYBR No-ROX One-Step Kit 4 x 100 rxns BIO-65053 BIO-98002 B3G1F SensiFAST SYBR No-ROX Kit BIO-73001 B3G1F SYBRSYBR Hi-ROX One-Step Kit Kit 4 x 100 rxns BIO-72001 B3G1F SensiFAST SensiFAST No-ROX One-Step SensiFAST cDNA Synthesis Kit BIO-74001 B3G1F SYBRSYBR Lo-ROX One-Step Kit Kit 4 x 100 rxns BIO-73001 B3G1F SensiFAST SensiFAST Hi-ROX One-Step BIO-82002 B3G1F SensiFAST Probe Hi-ROX Kit 4 x 200 rxns BIO-74001 B3G1F SensiFAST SYBR Lo-ROX One-Step Kit 50 reactions BIO-84002 B3G1F SensiFAST Probe Lo-ROX Kit 4 x 200 rxns BIO-82002 B3G1F SensiFAST Probe Hi-ROX Kit BIO-86002 B3G1F SensiFAST Probe No-ROX Kit Special Price €282 / £240 4 x 200 rxns BIO-84002 B3G1F SensiFAST Probe Lo-ROX Kit template, regardless of gene • FastcDNA results on all qPCR instruments abundance. Furthermore,just SensiFAST • Simple and reproducible, add primers and template Code cDNA Synthesis Kit Product will produce reliable, high performance results, in both real-time Size and conventional RT-PCR. BIO-86002 B3G1F X4 List Price € B3G1F Price € List Per €572 Unit B3G1F Price€429 € www.bioline.com/sensifast €572 4 x 200 rxnswww.bioline.com/sensifast €105 €315 €429 €572 4 x 200 rxns €105 €315 €429 4 x 200 rxns 4 x 100 rxns 4 x 100 rxns 4 x 100 rxns €740 €105 €740 €127 €315 €555 €381 €555 €740 €127 €576 €127 €381 €555 €381 €432 €576 4 x 200 rxns €432 €105 €315 www.bioline.com/sensifast €576 €432 SensiFAST Probe No-ROX Kit 4 x 200 rxns €105 €315 4 x 200 rxns €105 €315 NEW! CHOgro® Expression System from Mirus The Transfection Experts High titer transient transfection system for suspension CHO cells 3 Medical Supply Company, Damastown, Mulhuddart, D. 15 T: 01 822 4222 F: 01 822 4100 E: [email protected] W: www.medical-supply.ie The CHOgro® Expression System was developed through systematic optimization of transfection protocol parameters including: cell density, transfection reagent, media formulation and culture temperature 200 Code Product MIR 6260 CHOgro® Expression System CHOgro® Expression System 180 CHOgro® Expression Medium Freestyle™ CHO Expression Medium 160 140 IgG (mg/l) • Efficient - Enables high protein titers with simple workflow • Convenient - Quick adaptation to CHO cell lineages • Optimized - High density growth with minimal cell clumping post transfection • Worry-free – No commercial license required; animal origin free 120 100 Freestyle™ System 80 60 40 20 0 Cells/ml at the time of Transfection 1x106 2x106 TransIT-PRO®(1:1) 4x106 1x106 2x106 4x106 Freestyle™Max (1.25:1.25) Price € €890 Includes: • CHOgro® Expression Medium • CHOgro® Complex Formation Solution, L-Glutamine • Poloxamer 188 Solution and • 1ml TransIT-PRO® Transfection Reagent MIR 5700 TransIT-PRO® Transfection Kit, 1ml €591 Medical Supply Company, Damastown, Mulhuddart, D. 15 T: 01 822 4222 F: 01 822 4100 E: [email protected] W: www.medical-supply.ie 3 MojoSort™ is a magnetic bead-based system for effective cell isolation. Cells can be isolated through positive selection or negative selection using our antibody-conjugated magnetic nanobeads. Following separation with our magnet, both fractions of cells can be used for downstream applications. MojoSort™ Features • Tube-based magnetic separation. • Fast and easy isolation of pure, functional cells. • Outstanding pricing. • High yield. Targets Available CD3, CD4, CD8, CD14, CD45 Streptavidin and the Mouse Hematopoietic Progenitor Cell Isolation Kit (CD3E, CD45R/B220, Ly-6G/Ly-6C (Gr-1), CD11b, TER-119) Exclusive of any other Biolegend offers http://www.biolegend.com/mojosort New Glycan, Phopho PTK, Allergen, Glycome and Lectin pathway arrays! • • • • • • • • Product Highlights of Raybiotech Arrays More cost-effective than traditional ELISA Analyse from 8-1000 proteins per sample High specificity and system reproducibility Suitable for diverse sample types Low sample volume requirement: 50 µL or less Get results same day (6-hour processing time) Well-suited for high throughput assays Code Product Price € GA-Glycan-100-1 Glycan Array 100, simultaneous detection of binding partners for 100 different glycans. 4 sample kit €877 GAH-GCM-1000-2 Human Glycosylation Array 1000, simultaneous detection of the glycosylation protein profile of 1000 human proteins. 2 sample kit €2,724 GA-Lectin-40-8 Lectin Array 40, simultaneous detection of glycan profiles using 40 lectins. 8 sample kit €705 PAH-AGN-G1-4 Human Allergen Protein Array (glass slide) contains 48 common allergen proteins for simultaneous detection of human allergens from foods, animals, trees, grass, pollen, mites, fungi (molds), and insects. €452 Contact [email protected] for more details 4 Medical Supply Company, Damastown, Mulhuddart, D. 15 T: 01 822 4222 F: 01 822 4100 E: [email protected] W: www.medical-supply.ie 10% OFF ELISAassays Over 9,000 available on a huge number of species! Species Available: Human Rabbit Cat (Feline) Other Mammals Mouse Goat Guinea Pig Plant Rat Canine (Dog) Fish Bovine (Cow) Sheep (Ovine) Aquatic Porcine (Pig) Horse (Equine) Lizards Chicken Monkey Avian Visit www.ELISAassays.com today for more information. 2 for 1 Special Offer: Fluorofire-Blue ProViaTox kit Obtain dynamic proliferation, viability and cytotoxicity data with this new single reagent from Molecutools Using Alamar blue or CellTiter-Blue? Then switch and save with this ultra-sensitive reagent! What can I measure? Is it sensitive? How does it work? How do I measure? Measure Cell proliferation, cytotoxicity and viability in adherent and suspension cells Resazurin is reduced by metabolically active cells and converted into a coloured product (resorufin) Item No. A00038 A00038s Item Description FluoroFire-Blue ProViaTox Assay Free Sample: FluoroFire-Blue ProViaTox assay Yes. It can measure down to 100 cells/well in a 96 well plate Plate reader: fluoresence @ 530nm/590nm or colorimetric @950nm Pack Size 2 x 1000 Assays 100 Assays Special Price € 100 Free Fill out an enquiry form to request a free sample: www.molecutools.com/contact-details/ Medical Supply Company, Damastown, Mulhuddart, D. 15 T: 01 822 4222 F: 01 822 4100 E: [email protected] W: www.medical-supply.ie 5 New Clontech Guide-it™ products for CRISPR/Cas9 genome modification Features • Three kits for simple evaluation of the efficacy of sgRNA (single guide RNA) for Cas9-mediated gene editing studies • In vitro transcribe > 4μg of sgRNA per reaction for transfection or in vitro screening • Guide-it Mutation Detection Kit contains all reagents for PCR-based identification Using Guide-it technology to perform genome editing. A single guide RNA (sgRNA) consists of a crRNA sequence of insertions or deletions generated by zinc-finger nucleases (ZFNs), TALENs or that binds to a specific DNA target, and a tracRNA sequence that binds to Cas9 protein. When an sgRNA engineered to CRISPR/Cas9 contain a target sequence of interest binds to a recombinant • Guide-it Complete sgRNA Screening System (Cat. No. 631439) allows you to both form of Cas9 nuclease that has double-stranded DNA nicking activity, the resulting complex will produce target-specific produce sgRNA and test its ability to cleave its putative target in vitro double-stranded cleavage. Cellular repair, which is errorprone, will take place at the cleavage site, and may result in a • The Guide-it Indel Identification Kit provides a complete workflow for identifying mutation that can “knock out” a gene. the variety of insertions and deletions (indels) created using nuclease based genome editing technologies, including CRISPR/Cas9, TALENs, and zinc finger nucleases, in a cell population Item No. Item Description Price € 631438 Guide-it™ sgRNA In Vitro Transcription Kit (10 Rxns) 395 631439 Guide-it™ Complete sgRNA Screening System 705 631440 Guide-it™ sgRNA Screening Kit (30 Rxns) 442 631444 Guide-it™ Indel Identification Kit (10 Rxns) 421 631448 Guide-it™ Mutation Detection Kit (25 Rxns) 228 632607 Guide-it™ Cas9 Polyclonal Antibody (100 uL) 250 CRISPR Contact [email protected] for more details 6 Medical Supply Company, Damastown, Mulhuddart, D. 15 T: 01 822 4222 F: 01 822 4100 E: [email protected] W: www.medical-supply.ie Save 10% on Takara PCR Enzymes and Premixes Our suite of Takara PCR polymerase products is backed by 25 years of research and development, to ensure reliability and quality. This promotion includes many of our most popular enzymes. EmeraldAmp® and SapphireAmp® • Loading dye added • Optimized for great performance • Hot start premixes available • Convenient for both standard and high-throughput applications • Rapid results - no compromise on sensitivity, specificity, or efficiency Takara Taq™ Polymerases • Robust enzyme for standard PCR amplification • Proofreading activity • Perform routine applications without compromising specificity or efficiency • Amenable to high-throughput screening projects Cat. # Product Size List Price Special Price TaKaRa Taq Hot Start Version 250 units €143.00 R300A TaKaRa Taq HS Perfect Mix for fast reactions 100 reactions €105.00 €95.00 RR070A SpeedSTAR™ HS DNA Polymerase 250 units €228.00 €205.00 RR310A EmeraldAmp GT PCR Master Mix 160 rxns €84.00 €76.00 RR330A EmeraldAmp MAX HS PCR Master Mix 160 rxns €115.00 €104.00 RR350A SapphireAmp Fast PCR Master Mix 160 rxns €115.00 €104.00 PCR PRODUCTS R007A €129.00 Prices are subject to change without notice. www.clontech.com 06.14 US (633491) Medical Supply Company, Damastown, Mulhuddart, D. 15 T: 01 822 4222 F: 01 822 4100 E: [email protected] W: www.medical-supply.ie 7 Save 10% on qPCR Premixes and RT Kits PrimeScript™Reverse Transcriptase is a recombinant, RNase H Minus enzyme developed by Takara Bio, Inc. • • • • PrimeScript RTase is robust and versatile Strong strand displacement and extension capability Full-length cDNA molecules (up to 12 kb) are generated RT reaction at 42°C, allows high specificity, high yield, and reduced risk of RNA degradation • Fast: RT reaction is done in 15 minutes SYBR® qPCR premixes are recommended for most real-time PCR applications, including gene expression analyses and high-throughput studies. • Compatible with fast real-time protocols and instruments • Run times are less than half the time of other manufacturer’s products • Speed does not compromise sensitivity, specificity, or efficiency. Cat. # Product Size List Price Special Price RT - PCR & qPCR Products RR036A PrimeScript™ RT Master Mix (Perfect Reat Time) 200 rxns €365.00 €329.00 RR047A PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Reat Time) 100 rxns €367.00 €330.00 RR390A Premix Ex Taq (Probe qPCR) 200 rxns €240.00 €216.00 RR420A SYBR Premix Ex Taq (Tli RNaseH Plus) 200 rxns €287.00 €258.00 RR820A SYBR Premix Ex Taq II (Tli RNaseH Plus) 200 rxns €287.00 €258.00 638322 SYBR® Advantage® GC qPCR Premix 40 rxns €97.00 €87.00 638321 SYBR® Advantage® 50 rxns €66.00 €59.00 qPCR Premix Prices subject to change without notice. Only 1 order per lab. www.clontech.com 8 Medical Supply Company, Damastown, Mulhuddart, D. 15 T: 01 822 4222 F: 01 822 4100 E: [email protected] W: www.medical-supply.ie
