HERD project: STUDY OF THE MICROBIOLOGICAL FLORA OF

HERD project: STUDY OF THE MICROBIOLOGICAL FLORA OF

HERD project:
STUDY OF THE MICROBIOLOGICAL FLORA OF
MILK AND DAIRY PRODUCTS IN KOSOVO
The microbial flora (quality) of (i) milk and (ii)dairy
products in Kosovo: With emphasis on the dairy lactic acid
bacteria and the two pathogens Listeria monocytogenes
and Staphylococcus aureus
Research
Education
Information
Farmers
and
Company
Institutions and People Involved
•
•
•
•
University of Pristina:
Ibrahim Mehmeti, Post. Doc.
Skender Muji, Professor
Hysen Bytyqi, Professor
2 Master student (right now)
– Selami Salihu (Master)
– Ibadete Krasniqi (Master)
– Imer Haziri (PhD student)
3 Bachelor student (Diploma for Bachelor)
Norwegian University of Life Sciences:
Ingolf F. Nes, Professor
Dzung Bao Diep, Professor
Technical Support
EDUCATION
Master student
• Ibadete Krasniqi (UP- Pristina Kosovo)
• Selami Salihu (Agriculture University - Tirana
Albania)
Bachelor student
1. Remzi Jetullahu
1. Valdet Thaqi
2. Idriz Lalloshi
…
Work Training at LMG-UMB
1.Imer Haziri (PhD student)
1.Ibadete Krasniqi Master student
Laboratory Manuel
• Microbiology and food microbiology
– Agriculture Faculty and Veterinary used for
students, farmers and company
RESEARCH
RESULTS
I. Isolation and characterization of
bacteria in milk obtained from
producers
Sampling of milk
for bacterial analysis
Communities: 10
Farms: 237
Number of samples: 711 (3 tomes from each farm)
In December-February (237 samples)
In March- April (237 samples)
In May-June (237 samples)
Bacterial isolates: 5000
Samples collection
Podujeva
Skenderaj
Istogu
Skenderaj
Sharri
Lipjanë
Rahovec
Istogu
Rahoveci
Gjakova
Theranda
Ferizaji
Sharri
Sharri
Total Viable Count
TVC /mL
35
28% complyies
with the regulation
30
25
20
35,29
15
10
5
4,99
6,33
9,42
7,6
6,91
5,48
5,88
5,49
6,47
5,14
0
< 1… 100 000 < 100 001…
200 000
< 200 001300 000
< 300 001… < 400 001… < 500 001… < 600 001… < 700 001… < 800 001… < 900 001… < 1 000 000...
400 000
500 000
600 000
700 000
800 000
900 000
1000 000
Analyses
• 5000 different strains are collected
– 3000 Analyses in Norway
– 888 are analyzed for biochemical testes:
–
–
–
–
Growth at different pH (6.0 and 9.2)
Growth at different NaCl comcentration (2% and 6.4%)
Growth in two different mediums: MRS and GM17
Gas production(homo-fermentative and hetero-fermentative)
– 888 LAB for bacteriocin production
– Multi - Locus – Sequencing -Typing (MLST)?
Classification
Present status of 3000 isolates:
• Staphylococcus aureus: 235 isolates
• Listeria spp. 112 isolates
• Lactic acid bacteria: 2653
Meeting and visits in farms
• Farms
– Higher number (more contaminations)
– Between higher and normal
– Low number (according to regulation)
– Milk in company
Higher number of microorganisms
• Bad hygiene;
• No pipline desinfection;
• Hygiene of the farms;
Between extreme and limits
Good quality
 Good hygiene
 Good ventilation
 Pipeline in good conditions
Bacteriocins activity
• 576 samples are tested;
• 10 % are positive
• Indicators:
– Staphilococcus aureus
– Listeria innocua
– Bacillus cerus
– Lactococcus lactis
– Lactobacillus sakei
Presentations
• Week Science in Kosovo
– Two oral presentations:
– Ibrahim Mehmeti and
– Ibadete Krasniqi
– Conference in AlbShkenca organized in Macedonia
– Ibadete Krasniqi oral presentation and Poster
Future work
Samples collection
company
Skenderaj
Sharri
Lipjanë
Rahovec
Podujeva
Istogu
Skenderaj
Istogu
Rahoveci
Gjakova
Theranda
Ferizaji
Sharri
Sharri
Analysis of dairy products
Workshops
• Two workshops next summer organized with
farmers and company;
• Results presentation;
• Gust from Norway and Kosovo
Equipments
Thank you for your attention