June 24–30, 2016 - American Foundation for Donation

Affiliation:_________________________________________________
Business Address:___________________________________________
_________________________________________________________
City, State, Zip:_____________________________________________
Phone:____________________________________________________
Email:____________________________________________________
Emergency Contact (Name & Phone): _________________________
_________________________________________________________
Check one:
h Director
h New Laboratory Director
h Laboratory Manager
h Laboratory Supervisor
h Technologist
h Other: _____________________
A check must accompany this form to ensure registration.
Make checks payable to American Foundation for Donation and
Transplantation. Credit cards are accepted.
Mail check & form to: AFDT, 8154 Forest Hill Avenue, Suite 3
Richmond, VA 23235-3255
Registration Fees (in US dollars)
Before May 15
h$1,600.00
After May 16
h$1,800.00
Optional Review Session
h $150.00
(Mon., June 27, 12-2:30 pm)
Refunds will be made when requested in writing before April 30,
2016. A $100 fee will be charged on all refunds. There will be no
refunds after May 15, 2016.
For information and registration, contact:
Arlene M. Skinner, Manager
Continuing Education & Conference Planning
8154 Forest Hill Avenue, Suite 3,Richmond, VA 23235-3255
Tel: 804-323-9893; Fax: 804-323-1300; Email: [email protected]
Note: Hotel and travel arrangements and their costs will be the
responsibility of course participants. Registration will be Friday,
June 24, 2016 and course will end on Thursday, June 30 at
12 noon. Breakfast, lunch and breaks will be provided each day
except lunch on Monday, June 27, UNLESS you are participating
in the optional review session. Box lunches will be provided for
these participants. On Wednesday, June 29, dinner will be
provided during a Farewell Gathering.
Telephone: 1-210-227-3241 – Reference your attendance at the
AFDT Histocompatibility Specialist Course. Cutoff for group rate:
June 3, 2016.
Room rates: $159 single or double occupancy, plus 16.75% tax.
Presorted
First-Class Postage
Paid
Richmond, VA
Permit No. 681
Name: ____________________________________________________
Highly recommended review:
1. Reviews in Immunogenetics, Vol. 1 and Vol. 3, 1999, Munksgaard,
Copenhagen, Denmark.
2. Handbook of Human Immunology. 1997 Leffell, Donnenberg and Rose,
CRC Press.
3. Manual of Clinical Laboratory Immunology, Fifth Edition, 1997, eds:
Rose, deMacario, Fold, Lane and Nakamura, ASM Press, Section R:
“Transplantation Immunology and Immunogenetics” and Zachary, AA and
Steinberg, AG: Statistical Analysis and Applications of HLA Population Data,
pp. 132-140.
4. The HLA Facts Book, 2000, Steven G.E. Marsh, Peter Parham and
Linda D. Barber, Academic Press, San Diego, CA.
5. HIPAA (http://hhs.gov/news/press/2001/01privacy.html) pp. 1-50.
Reading for DNA & Stem Cell Lectures:
1. Hurley, C. DNA methods for HLA typing. http://www.dodmarrow.org under
scientific services/educational material. 2008.
2. Hurley, C. Acquisition and use of DNA-based HLA typing data in bone
marrow registries. Tissue Antigens 49:323, 1997.
3. Holdsworth, R. et al. The HLA Dictionary 2008: a summary of HLA-A, -B,
-C, -DRB1/3/4/5, -DQB1 alleles and their association with serologically
defined HLA-A, -B, -C, -DR and -DQ antigens. Tissue Antigens 73:95, 2009.
4. Marsh, S. et al. Nomenclature for factors of the HLA system, 2010. Tissue
Antigens, in press.
5. Robinson, J. et al. IMGT/HLA and IMGT/MHC: sequence databases for
the study of the major histocompatibility complex. Nucleic Acids Research
31:311, 2005.
6. ASHI Standards related to DNA-based HLA testing.
7. Cutler, C. and J. Antin. An overview of hematopoietic stem cell transplantation. Clinics in Chest Medicine 26:517, 2005
8. Copelan, E. Hematopoietic stem-cell transplantation. New England Journal
Medicine 354:1813, 2006
Eighteenth
Histocompatibility
Specialists Course
ABHI Approved
Recommended review:
1. ASHI Laboratory Manual, 2001 4th Edition, Editors: Hahn, A., et al.,
(Pub. ASHI, 17000 Commerce Parkway, Suite C, Mount Laurel, NJ 08054).
2. Flow Cytometry First Principles, 1992, Givan, Alice, (Pub. Wiley-Liss,
New York).
3. Flow Cytometry Principles for Clinical Laboratory Practice: Quality
Assurance for Quantitative Immunophenotyping, 1995, Marilyn Owensand,
Michael Loken (Pub.Wiley-Liss, New York).
4. “Flow Cytometric Crossmatching in Solid Organ Transplantation”, 1994, RA
Bray, In Methods of Cell Biology: Flow Cytometry, Vol 41, Darzynkiewics,
Z., Crissman, HZ, and Robinson, JP eds. Chapter 7, pp 103-119.
5. Bray R., Nickerson P., Kerman R., and Gebel H. Evolution of HLA Antibody
Detection: Technology Emulating Biology. Immunologic Research 29:41-53, 2004.
6. Bray R.A. and Gebel H.M. Clinical Utility of Flow Cytometry in Allogeneic
Transplantation. in; Flow Cytometry in Clinical Diagnosis, 4th Ed.; Carey J.
McCoy J. and Keren D. eds. pgs. 275-294. ASCP Press, Chicago,IL. 2007
June 24–30, 2016
Sheraton Gunter Hotel
SAN ANTONIO, TX
American Foundation for Donation and Transplantation
8154 Forest Hill Avenue, Suite 3
Richmond, VA 23235-3255
American Foundation for Donation and Transplantation
Histocompatibility Specialist Course Registration
Please print or type.
Sponsored by
American Foundation for Donation and Transplantation
(formerly SEOPF)
PURPOSE: The curriculum is designed for senior technologists and
new laboratory directors involved in the day to day management
and development of the histocompatibility laboratory. Applicants
should have a background in immunology, immunogenetics and
histocompatibility.
OBJECTIVE: Program objective is to establish a structured
curriculum leading to:
• Raising the standards of practice in clinical
histocompatibility testing.
• Providing a fundamental educational program leading to
ABHI Histocompatibility Specialist Certification (CHS).
• Promoting excellence in clinical laboratory medicine.
ORGANIZING COMMITTEE
Chair
Jean Heneghan, MS, CHS
Telephone: (504) 988-5259
[email protected]
Tulane HLA Laboratory
New Orleans, LA
Deborah Crowe, Ph.D.
Director
DCI Laboratory, Inc.
Nashville, TN
Inquiries about the program should be addressed to Arlene Skinner,
Director of Operations, 1-800-KIDNEY9, [email protected]
SYLLABUS: Each student will receive a comprehensive
course syllabus containing:
• Lecture summaries
• References and bibliography
• Procedures with applications
CURRICULUM: Course presentation will be in didactic and
interactive discussion format. The curriculum focus is on
histocompatibility testing:
• Theory and principle
• Application
• Procedure
• Management
APPLICANT REQUIREMENTS: Technologist in
Histocompatibility Testing, with a minimum of 3 years working
experience and/or a formal course in immunology or genetics; or
a new laboratory director; active ASHI membership (recommended);
and pre-requisite reading completed.
This course will have a limited number of registrants; early registration
is recommended.
HISTOCOMPATIBILITY SPECIALIST CURRICULUM 2016
FRIDAY, JUNE 24
Registration 5:00 - 8:00 pm
SATURDAY, JUNE 25
Basic Concepts of
Transplantation Immunology
•“Laws” of Transplantation
•History & Its Impact
•Adaptive Immunity & Molecular
Basis of Allorecognition
• Innate Immunity
Innate Immunity
• Pattern Recognition Receptors
•Inflammation
• Cytokines and Chemokines
• NK Cells and NK Receptors
Adaptive Immunity
•Immunoglobulin & TCR Gene
Rearrangement
•TCR, MHC & Accessory
Molecule Interactions
•T Cell Activation & Effector
T Cells
•B Cell Activation & Antibody
Production
Molecular Biology Techniques –
HLA Typing
•DNA Structure and Preparation,
Denaturation/Annealing,
•Oligonucleotide Probe
Hybridization
•Labeling/Detection
•DNA Synthesis; PCR; DNA
Sequencing
DNA-Based HLA Typing Methods
• SSOPH; SSP; SBT
•Techniques to Identify
HLA Alleles
HLA Informatics
•Accessing the Tools on the
IMGT/HLA and Other Websites
• Matching within a Family
•Resolution of Typing for
Unrelated Search
•Results of Unrelated Donor /
Cord Blood Search
•Mismatched Donor Based on
DP Epitope Matching
•Review Homework on Web
Tools
Quality Assurance and Quality
Control for DNA-Based Testing
•Various Steps in the DNA
Typing Process
• Problem Solving
Hematopoietic Stem Cell
Transplantation
•Fatal Blood Diseases Treated
with Transplantation
• HSC transplantation Procedure
• Sources of Stem Cells
• Donor Selection Criteria
• Donor Search Process
•Laboratory Tests-Patient
& Donor
Typing Case Studies
SUNDAY, JUNE 26
Immunoregulation & Tolerance
• Thymic Selection
•Activation vs. Inhibition:
Signals & Pathways
•Apoptosis and ActivationInduced Cell Death
• Clonal Deletion
• Regulatory Cells
HLA Disease Associations
•Characteristics of HLA
Associated Diseases
• Molecular Mechanisms
•Other susceptibility genes –
cytokine, KIR
•Commonly ordered tests for
Disease Association
•HLA Association with Drug
Hypersensitivity
Genetic Concepts Applicable
to Immunogenetics
• Gene Regulation and Expression
•Gene Transmission – Mendelian
Genetics
• Genetic Change
• Genomics and Proteomics
Population Genetics and Statistics
•Calculation of Gene (Allele)
Frequencies (GF) and
Phenotype Frequencies (PF)
• Hardy-Weinberg (HW) Testing
•Haplotype Estimation and
Linkage Disequilibrium
• Tests of Selection
• Genetic Distance
•Contingency table testing
and related statistics
• Logistic Regression
• Family-based association tests
MHC Genetics
•MHC Gene Complex – Loci,
Gene Classes, Non-MHC Genes,
Class I and Class II Genes
•Organization, ProductionStructure, Expression,
Regulation
•HLA Polymorphism, Variable
Sites, Generation, Maintenance,
Linkage Disequilibrium,
Cytokine Gene Polymorphism
MONDAY, JUNE 27
Solid Organ Transplant
Immunology
•Allograft Rejection: types and
diagnosis
•Clinical Approaches to
Tolerance Induction
•Immunomodulation
•Pathology
• Allograft pathology
•Methods of assessing graft
function
Clinical Intervention in
Transplantation
•Causes of ESRD, indications
and contraindications for
transplantation
•Immunosuppression Protocols:
standard and new drugs, steroid
sparing
• Targets of Immunosuppression
•Role of the laboratory in
supporting clinical practice
KPD Programs
Free Afternoon and Evening
Optional Tutorial for
Diplomate Exam
(12:00 pm – 2:30 pm)
TUESDAY, JUNE 28
Next Generation Sequencing
Basic Principles of Flow
Cytometry
Basic Concepts of the Flow
Advanced Flow Cytometry
•Application of Flow Crossmatch
Pre and Post Transplant
• Flow Specificity Beads
• Test Interpretation
• QC in Flow Cytometry
Complement-Dependent Assays
• CDC Assays for Crossmatch
•Interference with Extrinsic
Factors
• C’ QC
Solid Phase Immunoassays
• Interpretation Issues
• Problems and Pitfalls
• Defining Unacceptable Antigens
• Virtual Crossmatches
Patient Antibody Issues
•Relevance of Antibodies and
Antibody Characteristics
• Public, CREG, Epitopes
•Auto-Antibodies
• Non-HLA Abs
• Significance of C4d deposition
• C4d and C1q assays
Epitope Analysis
Antibody / XM Case Studies
WEDNESDAY, JUNE 29
Antibodies: Mechanism of Action
& Tools
• Antibody and Complement
•C’ Activation and
Accommodation
• Endothelial Crossmatch
•Tetramers for Quantifying
B Cells
Transplanting the Sensitized
Patient
•Desensitization
• Positive Crossmatch
• Plamapheresis, IVIg
• ABO Incompatible
•Risk Factors for Antibody
Mediated Rejection
• Donor Kidney Exchange
Pharmacogenetics
Chimerism Testing
•Methods using tandem repeats
(STR/VNTR)
•Determining chimerism in cell
lineages
• Other methods
Allocation of Organs from
Deceased Donors
•New / Update OPTN Policies
for HLA Typing/KPD
•Recent Advances in CPRA
and HLA Typing
•Impact of New Kidney
Allocation System
Emerging Technologies in the
HLA Laboratory – Sponsor
Presentations
THURSDAY, JUNE 30
Implementing an Effective QA
Program
• Designing a QA Program
•Quality Indicators and
Monitoring
• Root Cause Analysis
•QAPI
• Competency Assessment
Laboratory Accreditation
•Inspection Process – ASHI
and CAP
•Other Regulatory AgenciesCMS, JC, UNOS, NMDP,
OSHA, FDA
Laboratory Management/Cost
Report
Laboratory Safety
•OSHA
•Shipping
• Specimen Handling