SkyScan 1278
Transcription
SkyScan 1278
\X) BRUKER TECHNICAL SPECIFICATIONS X-ray sou rce 20-65kV, 50W, 501Jm spot size, 4-position filter changer X-ray detector flat-panel CMOS sensor, 1944 x 1536 pixels, 14-bit Nominalresolutio n (pix el size on th e object) <521Jm at any location in the scanned volume Scanning space 80mm in diameter, 200mm in length (80mm in diameter, 80mm in length in a single scan) Reco nst ructed vo Ium e up to 1536 x 1536 x 1566 pixels (after a singl e scan) lntegrated physiological monitoring real-time motion detection (5Mp co lour CMOS camera}, ECG, breat hing detection, temperatu re stabi lizatio n, all signals digitized in 16bit with up to 150 samples/sec Radi ation saf ety <1uSv/h at any point on the instrum ent surface Dim ensions, w eight 850W X 12400 X 960 H mm, 240kg Power supply 100-240V AC, 50-60Hz, <150W (exc luding w o rkst ation) LX.J Control workstation: Proces so rs Dual 8-core Intel XEO N Memory (RAM) 64G B I 1866MHz Di sk space (HDD) 6TB (2 x 3TB, RAIDO) + 512GB Solid State Drive Monitor 24"UitraSharp LED LCD (1920x1200 native resolution) 850mm 1240mm e - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - -- - SkyScan 1278 .z7 960mm www. bruker.com 1620mm • Ultra Low-Dose, High Throughput ln-Vivo X-Ray Microtomograph Bruker microCT Ksrtuizersweg 3B, 2SSO Kontid1, Belgium phone; +32 3 877 5705 fax: +32 3 877 5769 www.bruker-microct.com info@bruker-microclcom sales@bru ke•microclcom • Ci tothe Bruker n->tcroCTwebs!e please ., "''"'"'' ;can t " OR- :c I w«h your rroM> pto,... or oPad .- QR-reaci;o ""' a"'obble 011 tl"e AWSIO<IO Innovation with lntegrity MICROTOMOGRAPHY e WORLD'S FASTEST LOW-DOSE HIGH RESOLUTION IN-VIVO MICRO-CT • Shortest full-body animal scan ta kes 7.2 seconds with ty):ical dose less than 6mGy. • 50 11m nominal resdution throughout 80 mm scanning diametElf and 200 mm scanning length. • Fast and very sensitive flat-panel detector resdving dynsmies of mouse heart and lungs activities even without using a oontrast agent. • MicrofocusX-ray source with adjustable energy from 20 to 65kV and automatic filtEif changEif allo>Ns optimized scanning protoool with minimized applied dose. • Spatial beam shaper invented by Bruke r microCT reduces dose 2-5 tim es without affecting reoonstructed image quality. • lntegrated physiological monitoring with breathing sensor. ECG. temperature s tabilization and body movement detection. provides 4D (time-resolvedl cardiac and respiratory microtomography. lt can deliver more than 100 measuremen ts per second for all parameters and images. • Easily replaceable mouse and rat cassettewith anaes theti c gas ma sk, elec trical Connections to all physiological sensors can be inserted and removed by a single click. • On-screen dose meter indicates applied dose to the animal based on information from i nd ivi dua I ana Iysis of X-ray i ma ges. • lnteg rated toud1screen allows control of main system func tions by gloved hand s. • Reconstructed image data saved in DICOM and o ther conventiona l formatssuch as TIFF. JPG. BMP, PNG as weil as movies of 3D rend erin gs in AV I-format. • Results of reconstruction can be directly sent to m obi le devi ces, such as iPad, iPh one or Android phones/tablets for volume rendered 3D v iewing using supplied software. • The system is suppli ed with GLP (Good Laboratory Pra cticel software. e WORLD'S FASTEST LOW-DOSE HIGH RESOLUTION IN-VIVO MICRO-CT • Shortest full-body animal scan ta kes 7.2 seconds with ty):ical dose less than 6mGy. • 50 11m nominal resdution throughout 80 mm scanning diametElf and 200 mm scanning length. • Fast and very sensitive flat-panel detector resdving dynsmies of mouse heart and lungs activities even without using a oontrast agent. • MicrofocusX-ray source with adjustable energy from 20 to 65kV and automatic filtEif changEif allo>Ns optimized scanning protoool with minimized applied dose. • Spatial beam shaper invented by Bruke r microCT reduces dose 2-5 tim es without affecting reoonstructed image quality. • lntegrated physiological monitoring with breathing sensor. ECG. temperature s tabilization and body movement detection. provides 4D (time-resolvedl cardiac and respiratory microtomography. lt can deliver more than 100 measuremen ts per second for all parameters and images. • Easily replaceable mouse and rat cassettewith anaes theti c gas ma sk, elec trical Connections to all physiological sensors can be inserted and removed by a single click. • On-screen dose meter indicates applied dose to the animal based on information from i nd ivi dua I ana Iysis of X-ray i ma ges. • lnteg rated toud1screen allows control of main system func tions by gloved hand s. • Reconstructed image data saved in DICOM and o ther conventiona l formatssuch as TIFF. JPG. BMP, PNG as weil as movies of 3D rend erin gs in AV I-format. • Results of reconstruction can be directly sent to m obi le devi ces, such as iPad, iPh one or Android phones/tablets for volume rendered 3D v iewing using supplied software. • The system is suppli ed with GLP (Good Laboratory Pra cticel software. • Best results in any application FULL BODY MOUSE SCAf\1 A reconstructed coronal virtual slice through a mouse body. No contrast agent Scanning protocol: 65k\l, Al1mm filter, 501-Jm isotropic pixel size, 680x680x1732 pixels. USII\JG COI\JTRAST AGEI\JT Volume rendering froma full body mouse scanwith contrastagentinjection. Scanning protocol: 65kV, "low dose" filter, 501-Jm isotropic pixel size, 860x860x1944pixels SPII\JE MICROSTRUCTURE Volume rendering with virtual opening of the spine of a rat Scanning protocol: 65 kV, "low dose" filter, 501-Jm isotropic pixel size, 992x992x1566pixels FAT DISTRIBUTIOf\1 Af\JALYSIS Volume rendering ofthe distribution of fatshown in orange inside the mouse body. No contrast agent Scanning protocol: 60kV, Al 0.5mm filter, 501-Jm isotropic pixels. SCAI\Jf\Jif\JG OF MOUSE LUI\JGS Two identical sagittel slices reconstructedwithoutsynchronization with breathing !leftl and by 4D sorting using time marks from breathing sensor lrightl. No contrast agent TIME-RESOLVED HEART BEAT Coronal ltopl and sagittel lbottoml reconstructed slices from four phases of the cardiac cycle. 4D scanning using contrastagent with data sorting according to ECG time marks. • Best results in any application FULL BODY MOUSE SCAf\1 A reconstructed coronal virtual slice through a mouse body. No contrast agent Scanning protocol: 65k\l, Al1mm filter, 501-Jm isotropic pixel size, 680x680x1732 pixels. USII\JG COI\JTRAST AGEI\JT Volume rendering froma full body mouse scanwith contrastagentinjection. Scanning protocol: 65kV, "low dose" filter, 501-Jm isotropic pixel size, 860x860x1944pixels SPII\JE MICROSTRUCTURE Volume rendering with virtual opening of the spine of a rat Scanning protocol: 65 kV, "low dose" filter, 501-Jm isotropic pixel size, 992x992x1566pixels FAT DISTRIBUTIOf\1 Af\JALYSIS Volume rendering ofthe distribution of fatshown in orange inside the mouse body. No contrast agent Scanning protocol: 60kV, Al 0.5mm filter, 501-Jm isotropic pixels. SCAI\Jf\Jif\JG OF MOUSE LUI\JGS Two identical sagittel slices reconstructedwithoutsynchronization with breathing !leftl and by 4D sorting using time marks from breathing sensor lrightl. No contrast agent TIME-RESOLVED HEART BEAT Coronal ltopl and sagittel lbottoml reconstructed slices from four phases of the cardiac cycle. 4D scanning using contrastagent with data sorting according to ECG time marks. • Animal Handling • lntegrated Physiological Monitaring MOUSE AND RAT CASSETIES PHYS IOL OGI CAL M ONITO Rl NG The SkyScan 1278 system is supplied with exchangeable animal cassettes that can be used in all Bruker in-vivo imaging instruments such as MRI. micro-PET, micro-SPECl bio-luminescence, bioHuarescence, etc. to col lect multimodal information. lt allows co-registration of functional and morphological infarmation from the same animal. The physiological monitaring sub-system includes video monitaring of an animal with real-time movement detection, ECG and brea thing detection, and temperature stabilization . A 5 megapixel colour lV-camera is mounted above the animal bed and equipped with white LED illumination to produce a real-time image of the animal duri ng the scan. The sottware analyses the video stream from a user-selected area of the image, which th e operator can position on a part of the animal body where breathing movement is vi sible. These movements are converted into a movement waveform to provide brea thing timemarks for time-resolved micro-CT reconstructi on. Th e ca ssettes are equi pped with fa ce mask and tubes for anaesthetic gas as weil as w ith connectors for ECG electrodes and a temperature sensor. All tubes and contacts are com bin ed to a si ngl e conn ector, wh ich can be attached to or deta ched from the ani mal tran sport system by simple turn of a small slider. To prevent leakage of anaesthetic gas, corresponding connections have valves which stay closed if the animal casse tte is disconnected from the animal tra nsport sys tem . The face ma sk on the animal bed is connec ted to an air/gas flow sensor for direct brea thin g detecti on. The ECG el ectrodes in the anima I cassette are connected to a sensitive ECG amplifier. Both breathing and ECG signals are digitized, sent to computer and displayed as rea I-time profil es on-screen. An opera tor can select individual gain and threshold for each signal to optimise generati on of tim emarks. The monitaring also includes tempera ture stabilization by heated airflow, which maintains the scanned animal at a selected temperature, to prevent cooling of the animal under anaesthesia. ALL-CARBON ECG ELECTRODES The anim al cassettes in clu de special cablas an d cl ip electrodes to detect ECG signals by a sensi tive amplifier integrated in the physiological monitaring sub-system. The ECG connections use ECG wiring and electrodes specially developed by Bruker microCT which contain no metaI parts. The wires and electrodes employ advanced carbon-fiber conductive parts with X-ray absorption similar to that of animal tissues far uncompromised i mage quality. Shadow prOj&cbons (topland reconstructed slrces (bottom) throu~ a mous& body w1th attached meta I electrodes Oeft) and carbon alectrod&s developed by Bruker m 1croCT lnght) 4DTIME-RESOLVED MICROTOMOGRAPHY Physiological moni tonng creates reference ti memarks for time-resolved reconstru ction of heart and lung dynamics, invented by Bruker m icroCT. ln this scanning mode, multiple projecti on images ta ken at each gan try angular position are sorted pos t-scan in to brea thin g or heart tim e bi ns using recorded physiological monitaring tim emarks. Such sorting creates pseudo-static se ts of projections, which are reconstructed as separate datasets and produce 3D sets of results corresponding to different phases of the card iac or respiratory cycle. Our vi su ali za tion program Ioad s aII recons tru cted datasets and allows scrolling in XYZ dimension across the recons tru cted volum e as w eil as in the time-di men sion to dernon stra te dyna mies of heart or lung movements in sharp reconstructed images minimally affected by movement artifa cts. Because all acquired data are sorted after the acquisition process, respira tary and ca rdia c cycles can be visualized by sorting according to timemarks from physiological monitaring without rescanning theanimal. unages from camera IOI1 12131415161718191Ro 01112131415161?181 J~l timenallt I i ll II I I t I I I I I I I I I I I I I I I '- ~ I( f ./ " ' ICl Dl A B c IDIAIB CIDIA BICI DIAIB r\ 0~ breathing ' trm bins ~... r -1\0 -..,.., BO ., . .. CO (i > ö ++ + ~ g 00 + 1 ~J~1 J ~ ßft I I(~ 1 I ID1 - + -- + I- - + + --- - ,_ • Animal Handling • lntegrated Physiological Monitaring MOUSE AND RAT CASSETIES PHYS IOL OGI CAL M ONITO Rl NG The SkyScan 1278 system is supplied with exchangeable animal cassettes that can be used in all Bruker in-vivo imaging instruments such as MRI. micro-PET, micro-SPECl bio-luminescence, bioHuarescence, etc. to col lect multimodal information. lt allows co-registration of functional and morphological infarmation from the same animal. The physiological monitaring sub-system includes video monitaring of an animal with real-time movement detection, ECG and brea thing detection, and temperature stabilization . A 5 megapixel colour lV-camera is mounted above the animal bed and equipped with white LED illumination to produce a real-time image of the animal duri ng the scan. The sottware analyses the video stream from a user-selected area of the image, which th e operator can position on a part of the animal body where breathing movement is vi sible. These movements are converted into a movement waveform to provide brea thing timemarks for time-resolved micro-CT reconstructi on. Th e ca ssettes are equi pped with fa ce mask and tubes for anaesthetic gas as weil as w ith connectors for ECG electrodes and a temperature sensor. All tubes and contacts are com bin ed to a si ngl e conn ector, wh ich can be attached to or deta ched from the ani mal tran sport system by simple turn of a small slider. To prevent leakage of anaesthetic gas, corresponding connections have valves which stay closed if the animal casse tte is disconnected from the animal tra nsport sys tem . The face ma sk on the animal bed is connec ted to an air/gas flow sensor for direct brea thin g detecti on. The ECG el ectrodes in the anima I cassette are connected to a sensitive ECG amplifier. Both breathing and ECG signals are digitized, sent to computer and displayed as rea I-time profil es on-screen. An opera tor can select individual gain and threshold for each signal to optimise generati on of tim emarks. The monitaring also includes tempera ture stabilization by heated airflow, which maintains the scanned animal at a selected temperature, to prevent cooling of the animal under anaesthesia. ALL-CARBON ECG ELECTRODES The anim al cassettes in clu de special cablas an d cl ip electrodes to detect ECG signals by a sensi tive amplifier integrated in the physiological monitaring sub-system. The ECG connections use ECG wiring and electrodes specially developed by Bruker microCT which contain no metaI parts. The wires and electrodes employ advanced carbon-fiber conductive parts with X-ray absorption similar to that of animal tissues far uncompromised i mage quality. Shadow prOj&cbons (topland reconstructed slrces (bottom) throu~ a mous& body w1th attached meta I electrodes Oeft) and carbon alectrod&s developed by Bruker m 1croCT lnght) 4DTIME-RESOLVED MICROTOMOGRAPHY Physiological moni tonng creates reference ti memarks for time-resolved reconstru ction of heart and lung dynamics, invented by Bruker m icroCT. ln this scanning mode, multiple projecti on images ta ken at each gan try angular position are sorted pos t-scan in to brea thin g or heart tim e bi ns using recorded physiological monitaring tim emarks. Such sorting creates pseudo-static se ts of projections, which are reconstructed as separate datasets and produce 3D sets of results corresponding to different phases of the card iac or respiratory cycle. Our vi su ali za tion program Ioad s aII recons tru cted datasets and allows scrolling in XYZ dimension across the recons tru cted volum e as w eil as in the time-di men sion to dernon stra te dyna mies of heart or lung movements in sharp reconstructed images minimally affected by movement artifa cts. Because all acquired data are sorted after the acquisition process, respira tary and ca rdia c cycles can be visualized by sorting according to timemarks from physiological monitaring without rescanning theanimal. unages from camera IOI1 12131415161718191Ro 01112131415161?181 J~l timenallt I i ll II I I t I I I I I I I I I I I I I I I '- ~ I( f ./ " ' ICl Dl A B c IDIAIB CIDIA BICI DIAIB r\ 0~ breathing ' trm bins ~... r -1\0 -..,.., BO ., . .. CO (i > ö ++ + ~ g 00 + 1 ~J~1 J ~ ßft I I(~ 1 I ID1 - + -- + I- - + + --- - ,_ • Simple control, enjoyable experience • Software for reconstruction, analysis and realistic visualization TOUCHSCREEN CONTROL All mi cro-CT scanners from 8 ru ke r m icroCT, includi ng the SkyScan 12 78, are suppli ed wi 1h a comprehen sive softwa re suite and supported by unlimited free access to software updates available for download from the Bruke r microCTwebsite. The user interface of the SkyScan1278 system i s simple and intuitive. Th e in strum ent can be controlled from the computer screen an d aIso from the embedded force-sensitive touchscreen, which ca n be operated by gl oved ha nds. Th e tou eh screen allows sei ecti on of sca nni ng protocol, adju stin g anim al bed position and control of imaging and sca nni ng. Where multiple ani ma I sca ns are s tarted from the tou eh screen, the softwarewill automatically save acquired da ta to separate subfol ders with in cremen tally assi gned fol der n am es and dataset file prefixes. GPU-ACCELERATED RECONSTRUCTION The reconstruction softwareN Recon uses a unique parallel izati on alg orith m for GPU-a ccel erati on on sin gle or multiple gra ph ical processors. lt supports beamha rdenin g correction, mi sa lig nment compensati on, ring artifact removal, volum e of interes t recons tru ction, au tomatic merging partial sca ns and ma ny other options. VISUALIZATION OF RECONSTRUCTED RESULTS ......., ... ' ' lttt The visualization program DataViewer displays results as a sli ce-by-slice m o.tie or as three adja cently displayed orth ogona I section s, centred at any selected poin t. One can rota te and resa mple recons tructed da tasets in any di rection. Ad diti ona I fea tu res includ e th e 4th di men si on for time-resolved cardiac and respiratory tomography. lt aIso i ncl udes automa tic 2D an d 3D co-regi stra tion. Full flexibili ty of sca nner tun ctiona lity i s aeh ieved by a stra igh tforwa rd stan dard Mi erosoft Win dows user i nterfa ce on the control works tati on. lt allows flexible ad justment of all X-ray sou rce and detector setti ngs as weil as con trol of aII sca nn ing parameters. Any particular set of all in strum ent setting s can be saved as a user configuration, allowing reload of all setti ngs by one cl ick for future sca ns. 2D /3D IMAGE PROCESSING AND ANALYSIS VARIABLE X-RAY ENERGY, FILTERSAND BEAM SHAPER FOR LOW-DOSE SCANNING The SkyScan 1278 uses an X-rav source with adjustable energy end power. ln combination with a motorized fouf1)0sition filter changer it provides optimal scanning protocols for in-vivo studies taking into eocount size of animals. scanning site. gating end dose requirementB. One of the positions in the filterchangar is occupied b'( a so-called Nlow-doseN filter. which is e uniqua X-n1Y beem shaper invented b'( Bruker microCT (patent pendingl. lt createa variable filtering conditions ecross the scenning area end reduces dose to the enimal 2-5 times without compromising image qualitv of 1he reconstructed results. ON-SCREEN REAL-TIME DOSE METER The SkyS ca n1278 control software includ es a real-time on-screen dose meter. lt i ndi ca tes an es tima tion of th e dose absorbed by anim al body duri ng sca nn ing. Th e measurement is based on the absorption calculations from X-ray im ag es of the pa rt of th e anim al irra dia ted by X-rays durin g sca nni ng cross-ca librated with electron ic dosimeter measurements. The dose meter ca n show accu mul ated dose or d ose rate. Th e color of th e d ose di splay eh an ges from green to yell ow- orange - red to ind ica te i ncreasin g of accumu lated d ose or d ose rate, to help th e u ser Iim it the dose to the an ima I to an accepta bl e Ievei. The dose meter is calibrated for X-ray absorption in the s tan da rd mouse and rat cassettes. ln this way it only measures the X-ray dose absorbed in ani ma I body itsel f duri ng scann ing. The d ose absorbed by the ani ma I du ring a scan is documented in the scan log-fil e tog ether with all scan and reconstruction settings. CT-Ana lyser or "CTAn" allows accurate and detailed study of micro-CT results for morphometry and densitometry. Powerful, flexible and programmable ima ge processi ng tools allow a wi de rang e of segmenta tion, en ha ncemen t an d m ea suremen t functions for an alysi s i nsi de any sl ice or 3D vol ume. Versatile volume of interest selection tools allows free hand drawing, selection of standard shapes and editing regions of interests in key slices with au toma tic interpol ati on to full volum e. CTAn con ta ins hundreds of embedded functions with the possibility to execu te any seq uen ce of ta sks step by step, buil d tasklists for automated batch-analysis and execute user-crea ted plu g-i ns. -_ .·-..... l'I!IXIII- .·.,_ ·-.-,.., >J:ftrn"" lt:?l'"-"" 3DVISUALIZATION BY SURFACE RENDERING CT-Volume or "CTVol" uses surface-triangulated models from CTAn and provides a virtuai3D viewing environment, flexible and rich in features, to give you a wid e ra nge of options for 3D presenta tion of microCT results. Any surface- tri an gul ated volum e can be exported in STL-format for F EA analysis or for 3D printers to create a physical copy of the scan ned object. -- ~ · ,... : ~> ::.. ~> • • ." 3D VISUALIZATION BYVOLUME RENDERING The volume rendering program CTVox displays a set of recons tructed sli ces as a realis tic 3D obj ect wi 1h intuitive naviga tion and man ipu Ia tion of both object and ca mera, a flexible clippin g tool to produce cut-away views and an interactive tran sfer tun cti on con trol to ad jus t color and transparency. The lighting and shadowing with selection of properties of the material surfaces produces fully real isti c visua lizati on. A "flig ht record er" tu nction allows fast creation of "fly around" and "fly through" an ima tion s based on simple selection of several key frames with automatic interpolation in between. lma ging possibili ties in clu de stereo viewing an d displaying multiple datasets, obtained from single or different m oda liti es. " II'Diume rendetintl of a mocm: by CT\bx (ftoot ris;ht part vittually remcMd.l • Take your results anywhere • Comprehensive support and good practice for best results VOLUME RENDERING FOR MOBILES GOOD LABORATORY PRACTICE (GLP) SOFTWARE The volume rendEiring program supplied with the system, CTVox. has also its mobile versions. which can be downloaded for free from the AppStore for iPhonefiPadfiPod or from GooglePiay for Android devices. Any 30 results obtained ~ the system can be sent to a mobile device for realistic visualization ~ real-time volume rendering with 30 object manipulation. adjustments of opacity and colors. virtual cut. etc. The SkyScan1278 system is supplied with a GLP software. which when activated allows administration of user rights and implementing neoessary data protection according to GLP re quirements. Acoess to control software will be protected ~ a user's name · password combination . Two Ieveis of access can be granted: standard users and advanced users. Standard use(s rights allow scanning anim als with saving da ta. reading saved results and switching scanning protocols. but do not allow erasing or modifying scanned datasets or changing scanner settings. The users with advanced access rights have access to the full tu ncti ona Ii ty of the system . User pa ssword s and access righ ts are managed by a single system adminis trator. The results can be sent through a cable connection or wireless network . Th e exported rendered data and color sch emes are s tored in the local memory of the m obi le device and do not req uire any conn ecti on or downIoad ing du ring ma nipula tions. AI arge number of reconstructed datasets can be load ed to the mem ory o f a mobile dev ice. allowing you to study im ag e resul ts whil e travell ing. sha re them with colleagues and demoostra te at meetings. When the GLP software is activated, th e control Software dupli ca tes every scan Iog-fiie with all scan param eters and sys tem settings. in an encrypted copy, which canno t be directly accessed or m odified. Wh en n ecessary, encrypted Iog fi Ies can be restered to tex t for QA audit, to ensure the secure s toring and traceabili ty of criti cal sca n i nforma tion an d allow reprod ucti on of any sca n. AUTOMATIC E-MAIL REPORTING SOFTWARE UPDATES The SkyScan 1278 control softwa re can send you an ~ma il at the end of a scan . The ~mail indudes a direct link to the data telder containing the scan results . By a simply clidking this link you can open the dataset directly. lf the scanning process has been interrupted. the softwarewill also email you a report of the details. All u~~~era af Bruker mierocr instruments hsve unlimited free aeeess to all eoftwure updetes. New vera iona of control 1 nc1 1 pplieation aoftware ean be downloaded from the brulceHnieroet.com webaite. To go to the Bruk:er mieroCT webaite, juat eliek: on the link in the "About" box fn the control p.rogram. Using the other link in the eeme "About" boK, the operutor ee n ~~~end an 8-ffleil w ith questions or requesta to infotbrukeHnicroct.com. The ~mail notification can be flexibly configured according to local security rules for IT infrastructure. .._. -- ,................. __.,..,.._"_ ~.....t _ lA.O ..........,., ~- 0:<'.,~111!!1CWMn21.1C ~ kM-.....-~ TRAINING COURSES. MI CRO-CT ANNUAL MEETINGS, 'BRUKER MI CRO-CT ACADE MY' Bruker microCT offers e combinetion of both system end softv.11re trei ning thet COYBrs three mejor topics; imege acqu isition, image reconstruction end deta analysis/Yisualisation. These 5-day courses are held severel times per yeer at BrukBr microCT IMAGE FORMATS FOR RESULTS All supplied reconstruction and appli cation so ftware ca n produce and workwith DICOM format (compliantwi th the D ICOM 3 convention). wh ich can be consi dered as an ind ustry stand ard for medical ima ging instruments. The programs ca n also in terchange results in standard Windows-readable formats, such as BM P, JPG and TIFF for images and AVI for movie files. lf necessary, images can be converted from one format to another using the supplied Format Converter utility. This software can convert projection im ag es and recons tructed slices to another form at with modified size and intensity sca le. lt can convert separate image files or full da taset with rena mi ng, resizing. rescali ng an d ren umberin g for combining several sets of reconstructed slices together. Another useful supplied utility named DICOM-CT allows the export of datasets saved as JPG, BMP or TIFF files to standard OICOM format. headquerters in Belgium. The goel is to combine the basic theoretical baek:ground of microCT with es much hends-on experience es possible. After installetion of every system the new customers will raceive first initial training, end letar edvanced treining either on-site or by course attendence, et the usar's choica. Bruker microCT also organizes an ennual MicroCT maeting in the form of e 3-day scientific conhtrenoa combinad with treining wor1<shops. Intensive exchange of knowledge end experience helps new end skilled users to find the way to get the best results from their microCT imaging. lnvitation to the next MicroCT meeting and ebstracts from presentetions in the previous meetings ean be found at www.brukeF-microct.com .~. The "8 ru ke r m ic ro CT A cade my " is an efficien t edu ca tion al network for the hundreds of groups who are using SkyScan in struments. lt includ es a m onthly new sletter w ith appli ca tion and technical tips and keeps users updated on new methods. developments and company news. Through participation in the Academy our users gain access to a database with detailed appl ication and technical notes and provide feedbaok with qu estions and suggestions for improvements of our in struments and software. • Simple control, enjoyable experience • Software for reconstruction, analysis and realistic visualization TOUCHSCREEN CONTROL All mi cro-CT scanners from 8 ru ke r m icroCT, includi ng the SkyScan 12 78, are suppli ed wi 1h a comprehen sive softwa re suite and supported by unlimited free access to software updates available for download from the Bruke r microCTwebsite. The user interface of the SkyScan1278 system i s simple and intuitive. Th e in strum ent can be controlled from the computer screen an d aIso from the embedded force-sensitive touchscreen, which ca n be operated by gl oved ha nds. Th e tou eh screen allows sei ecti on of sca nni ng protocol, adju stin g anim al bed position and control of imaging and sca nni ng. Where multiple ani ma I sca ns are s tarted from the tou eh screen, the softwarewill automatically save acquired da ta to separate subfol ders with in cremen tally assi gned fol der n am es and dataset file prefixes. GPU-ACCELERATED RECONSTRUCTION The reconstruction softwareN Recon uses a unique parallel izati on alg orith m for GPU-a ccel erati on on sin gle or multiple gra ph ical processors. lt supports beamha rdenin g correction, mi sa lig nment compensati on, ring artifact removal, volum e of interes t recons tru ction, au tomatic merging partial sca ns and ma ny other options. VISUALIZATION OF RECONSTRUCTED RESULTS ......., ... ' ' lttt The visualization program DataViewer displays results as a sli ce-by-slice m o.tie or as three adja cently displayed orth ogona I section s, centred at any selected poin t. One can rota te and resa mple recons tructed da tasets in any di rection. Ad diti ona I fea tu res includ e th e 4th di men si on for time-resolved cardiac and respiratory tomography. lt aIso i ncl udes automa tic 2D an d 3D co-regi stra tion. Full flexibili ty of sca nner tun ctiona lity i s aeh ieved by a stra igh tforwa rd stan dard Mi erosoft Win dows user i nterfa ce on the control works tati on. lt allows flexible ad justment of all X-ray sou rce and detector setti ngs as weil as con trol of aII sca nn ing parameters. Any particular set of all in strum ent setting s can be saved as a user configuration, allowing reload of all setti ngs by one cl ick for future sca ns. 2D /3D IMAGE PROCESSING AND ANALYSIS VARIABLE X-RAY ENERGY, FILTERSAND BEAM SHAPER FOR LOW-DOSE SCANNING The SkyScan 1278 uses an X-rav source with adjustable energy end power. ln combination with a motorized fouf1)0sition filter changer it provides optimal scanning protocols for in-vivo studies taking into eocount size of animals. scanning site. gating end dose requirementB. One of the positions in the filterchangar is occupied b'( a so-called Nlow-doseN filter. which is e uniqua X-n1Y beem shaper invented b'( Bruker microCT (patent pendingl. lt createa variable filtering conditions ecross the scenning area end reduces dose to the enimal 2-5 times without compromising image qualitv of 1he reconstructed results. ON-SCREEN REAL-TIME DOSE METER The SkyS ca n1278 control software includ es a real-time on-screen dose meter. lt i ndi ca tes an es tima tion of th e dose absorbed by anim al body duri ng sca nn ing. Th e measurement is based on the absorption calculations from X-ray im ag es of the pa rt of th e anim al irra dia ted by X-rays durin g sca nni ng cross-ca librated with electron ic dosimeter measurements. The dose meter ca n show accu mul ated dose or d ose rate. Th e color of th e d ose di splay eh an ges from green to yell ow- orange - red to ind ica te i ncreasin g of accumu lated d ose or d ose rate, to help th e u ser Iim it the dose to the an ima I to an accepta bl e Ievei. The dose meter is calibrated for X-ray absorption in the s tan da rd mouse and rat cassettes. ln this way it only measures the X-ray dose absorbed in ani ma I body itsel f duri ng scann ing. The d ose absorbed by the ani ma I du ring a scan is documented in the scan log-fil e tog ether with all scan and reconstruction settings. CT-Ana lyser or "CTAn" allows accurate and detailed study of micro-CT results for morphometry and densitometry. Powerful, flexible and programmable ima ge processi ng tools allow a wi de rang e of segmenta tion, en ha ncemen t an d m ea suremen t functions for an alysi s i nsi de any sl ice or 3D vol ume. Versatile volume of interest selection tools allows free hand drawing, selection of standard shapes and editing regions of interests in key slices with au toma tic interpol ati on to full volum e. CTAn con ta ins hundreds of embedded functions with the possibility to execu te any seq uen ce of ta sks step by step, buil d tasklists for automated batch-analysis and execute user-crea ted plu g-i ns. -_ .·-..... l'I!IXIII- .·.,_ ·-.-,.., >J:ftrn"" lt:?l'"-"" 3DVISUALIZATION BY SURFACE RENDERING CT-Volume or "CTVol" uses surface-triangulated models from CTAn and provides a virtuai3D viewing environment, flexible and rich in features, to give you a wid e ra nge of options for 3D presenta tion of microCT results. Any surface- tri an gul ated volum e can be exported in STL-format for F EA analysis or for 3D printers to create a physical copy of the scan ned object. -- ~ · ,... : ~> ::.. ~> • • ." 3D VISUALIZATION BYVOLUME RENDERING The volume rendering program CTVox displays a set of recons tructed sli ces as a realis tic 3D obj ect wi 1h intuitive naviga tion and man ipu Ia tion of both object and ca mera, a flexible clippin g tool to produce cut-away views and an interactive tran sfer tun cti on con trol to ad jus t color and transparency. The lighting and shadowing with selection of properties of the material surfaces produces fully real isti c visua lizati on. A "flig ht record er" tu nction allows fast creation of "fly around" and "fly through" an ima tion s based on simple selection of several key frames with automatic interpolation in between. lma ging possibili ties in clu de stereo viewing an d displaying multiple datasets, obtained from single or different m oda liti es. " II'Diume rendetintl of a mocm: by CT\bx (ftoot ris;ht part vittually remcMd.l • Take your results anywhere • Comprehensive support and good practice for best results VOLUME RENDERING FOR MOBILES GOOD LABORATORY PRACTICE (GLP) SOFTWARE The volume rendEiring program supplied with the system, CTVox. has also its mobile versions. which can be downloaded for free from the AppStore for iPhonefiPadfiPod or from GooglePiay for Android devices. Any 30 results obtained ~ the system can be sent to a mobile device for realistic visualization ~ real-time volume rendering with 30 object manipulation. adjustments of opacity and colors. virtual cut. etc. The SkyScan1278 system is supplied with a GLP software. which when activated allows administration of user rights and implementing neoessary data protection according to GLP re quirements. Acoess to control software will be protected ~ a user's name · password combination . Two Ieveis of access can be granted: standard users and advanced users. Standard use(s rights allow scanning anim als with saving da ta. reading saved results and switching scanning protocols. but do not allow erasing or modifying scanned datasets or changing scanner settings. The users with advanced access rights have access to the full tu ncti ona Ii ty of the system . User pa ssword s and access righ ts are managed by a single system adminis trator. The results can be sent through a cable connection or wireless network . Th e exported rendered data and color sch emes are s tored in the local memory of the m obi le device and do not req uire any conn ecti on or downIoad ing du ring ma nipula tions. AI arge number of reconstructed datasets can be load ed to the mem ory o f a mobile dev ice. allowing you to study im ag e resul ts whil e travell ing. sha re them with colleagues and demoostra te at meetings. When the GLP software is activated, th e control Software dupli ca tes every scan Iog-fiie with all scan param eters and sys tem settings. in an encrypted copy, which canno t be directly accessed or m odified. Wh en n ecessary, encrypted Iog fi Ies can be restered to tex t for QA audit, to ensure the secure s toring and traceabili ty of criti cal sca n i nforma tion an d allow reprod ucti on of any sca n. AUTOMATIC E-MAIL REPORTING SOFTWARE UPDATES The SkyScan 1278 control softwa re can send you an ~ma il at the end of a scan . The ~mail indudes a direct link to the data telder containing the scan results . By a simply clidking this link you can open the dataset directly. lf the scanning process has been interrupted. the softwarewill also email you a report of the details. All u~~~era af Bruker mierocr instruments hsve unlimited free aeeess to all eoftwure updetes. New vera iona of control 1 nc1 1 pplieation aoftware ean be downloaded from the brulceHnieroet.com webaite. To go to the Bruk:er mieroCT webaite, juat eliek: on the link in the "About" box fn the control p.rogram. Using the other link in the eeme "About" boK, the operutor ee n ~~~end an 8-ffleil w ith questions or requesta to infotbrukeHnicroct.com. The ~mail notification can be flexibly configured according to local security rules for IT infrastructure. .._. -- ,................. __.,..,.._"_ ~.....t _ lA.O ..........,., ~- 0:<'.,~111!!1CWMn21.1C ~ kM-.....-~ TRAINING COURSES. MI CRO-CT ANNUAL MEETINGS, 'BRUKER MI CRO-CT ACADE MY' Bruker microCT offers e combinetion of both system end softv.11re trei ning thet COYBrs three mejor topics; imege acqu isition, image reconstruction end deta analysis/Yisualisation. These 5-day courses are held severel times per yeer at BrukBr microCT IMAGE FORMATS FOR RESULTS All supplied reconstruction and appli cation so ftware ca n produce and workwith DICOM format (compliantwi th the D ICOM 3 convention). wh ich can be consi dered as an ind ustry stand ard for medical ima ging instruments. The programs ca n also in terchange results in standard Windows-readable formats, such as BM P, JPG and TIFF for images and AVI for movie files. lf necessary, images can be converted from one format to another using the supplied Format Converter utility. This software can convert projection im ag es and recons tructed slices to another form at with modified size and intensity sca le. lt can convert separate image files or full da taset with rena mi ng, resizing. rescali ng an d ren umberin g for combining several sets of reconstructed slices together. Another useful supplied utility named DICOM-CT allows the export of datasets saved as JPG, BMP or TIFF files to standard OICOM format. headquerters in Belgium. The goel is to combine the basic theoretical baek:ground of microCT with es much hends-on experience es possible. After installetion of every system the new customers will raceive first initial training, end letar edvanced treining either on-site or by course attendence, et the usar's choica. Bruker microCT also organizes an ennual MicroCT maeting in the form of e 3-day scientific conhtrenoa combinad with treining wor1<shops. Intensive exchange of knowledge end experience helps new end skilled users to find the way to get the best results from their microCT imaging. lnvitation to the next MicroCT meeting and ebstracts from presentetions in the previous meetings ean be found at www.brukeF-microct.com .~. The "8 ru ke r m ic ro CT A cade my " is an efficien t edu ca tion al network for the hundreds of groups who are using SkyScan in struments. lt includ es a m onthly new sletter w ith appli ca tion and technical tips and keeps users updated on new methods. developments and company news. Through participation in the Academy our users gain access to a database with detailed appl ication and technical notes and provide feedbaok with qu estions and suggestions for improvements of our in struments and software. \X) BRUKER TECHNICAL SPECIFICATIONS X-ray sou rce 20-65kV, 50W, 501Jm spot size, 4-position filter changer X-ray detector flat-panel CMOS sensor, 1944 x 1536 pixels, 14-bit Nominalresolutio n (pix el size on th e object) <521Jm at any location in the scanned volume Scanning space 80mm in diameter, 200mm in length (80mm in diameter, 80mm in length in a single scan) Reco nst ructed vo Ium e up to 1536 x 1536 x 1566 pixels (after a singl e scan) lntegrated physiological monitoring real-time motion detection (5Mp co lour CMOS camera}, ECG, breat hing detection, temperatu re stabi lizatio n, all signals digitized in 16bit with up to 150 samples/sec Radi ation saf ety <1uSv/h at any point on the instrum ent surface Dim ensions, w eight 850W X 12400 X 960 H mm, 240kg Power supply 100-240V AC, 50-60Hz, <150W (exc luding w o rkst ation) LX.J Control workstation: Proces so rs Dual 8-core Intel XEO N Memory (RAM) 64G B I 1866MHz Di sk space (HDD) 6TB (2 x 3TB, RAIDO) + 512GB Solid State Drive Monitor 24"UitraSharp LED LCD (1920x1200 native resolution) 850mm 1240mm e - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - -- - SkyScan 1278 .z7 960mm www. bruker.com 1620mm • Ultra Low-Dose, High Throughput ln-Vivo X-Ray Microtomograph Bruker microCT Ksrtuizersweg 3B, 2SSO Kontid1, Belgium phone; +32 3 877 5705 fax: +32 3 877 5769 www.bruker-microct.com info@bruker-microclcom sales@bru ke•microclcom • Ci tothe Bruker n->tcroCTwebs!e please ., "''"'"'' ;can t " OR- :c I w«h your rroM> pto,... or oPad .- QR-reaci;o ""' a"'obble 011 tl"e AWSIO<IO Innovation with lntegrity MICROTOMOGRAPHY