unclassified

Transcription

unclassified

Entomological Surveillance Working Group
Alongkot Ponlawat, PhD
Chief of Vector Biology and Control Section,
Department of Entomology,
USAMC-AFRIMS
19 August 2015
8/18/2015
Armed Forces Research Institute of Medical Sciences
www.themegallery.com
UNCLASSIFIED
UNCLASSIFIED
1
www.afrims.org
Current Research Projects
GEIS
1. Malaria vector surveillance in western Thailand /Cambodia
MIDRP and others
1. Field evaluation of spatial repellents and attractants to develop an integrated pushpull system for Aedes aegypti control
2. Field evaluation of CO2 generators in Thailand
3. Evaluations of vector control devices under the semi-field conditions
4. CHIKV dipstick research project
5. Dengue study at KPP (PO1-NIH)
6. Wolbachia-mediated interference of dengue virus transmission
8/18/2015
UNCLASSIFIED
2
www.afrims.org
Malaria vector surveillance in western Thailand
Objective
•
To conduct malaria vector surveillance and evaluate malaria
infection rates in mosquitoes in western Thailand, along ThaiMyanmar border region
8/18/2015
UNCLASSIFIED
3
www.afrims.org
Study sites
Laos
Mae Ramat District,
Tak Province
1. Mae Ramat district, Tak
Myanmar
2. Sangklaburi district,
Kanchanaburi
Cambodia
Sangkhlaburi District,
Kanchanaburi Province
8/18/2015
UNCLASSIFIED
4
www.afrims.org
Ban Huay Pla Kong, Ka Ne Chih subdistrict, Mae Ramat
District, Tak Province
• 20 CO2-baited CDC light
traps/study site
• period 1700-0700 for 3
consecutive nights/month
Ban Mae Tew, Nong Lu subdistrict, Sangkhlaburi district,
Kanchanaburi province.
8/18/2015
UNCLASSIFIED
5
www.afrims.org
Results
Study site
Study trial
Total Anopheles females
An. minimus
An. dirus
An. maculatus
An. baimaii
An. sawadwongporni
An. philippinensis
An. kochi
An. barbirostris
An. barbumbrosus
An. hodgini
An. peditaeniatus
An. campestris
An. annularis
An. aconitus
An. culicifacies
An. dravidicus
An. harrisoni
An. jamesii
An. karwari
An. nivipes
An. notanandi
An. tessellatus
An. vagus
An. varuna
June
2,557
2,415
15
2
100
1
2
209
7
27
256
2
6
5
August
337
May
1,044
68
12
90
1
91
1
21
3
4
994
1
4
Sangkhlaburi
June
4,683
July
8169
3,331
6
386
7
157
348
337
52
5
6826
25
308
2
12
88
801
26
6
3
18
6
8
1
1
1
1
1
14
5
14
1
4
2
1
3
3
4
1
1
1
7
2
2
8
9
4
1
7
18
8
2
45
1
2
1
Other Anopheles species
Primary vector of malaria in Thailand
8/18/2015
Mae Ramat
July
530
3
2
1
1
34
3
3
1
1
UNCLASSIFIED
6
www.afrims.org
Results
Mae Ramat
51.95%
Aedes
Anopheles
17.87%
Culex
Mansonia
Others
27.33%
2.76%
0.09%
The proportion of mosquitoes captured from Mae Ramat district
during the malaria peak season in 2015.
8/18/2015
UNCLASSIFIED
7
www.afrims.org
Results
Sangkhlaburi
1.50%
3.04%
0.35%
30.10%
Aedes
Anopheles
Culex
Mansonia
Others
65.01%
The proportion of mosquitoes captured from Sangkhlaburi district
during the malaria peak season in 2015.
8/18/2015
UNCLASSIFIED
8
www.afrims.org
Population densities and malaria infection rates of
Anopheles species in malaria high-risk areas of Cambodia
Objective
•
To determine population densities of Anopheles spp. in selected
habitats (forested and deforested areas) of malaria high-risk areas,
Cambodia
•
To determine malaria infection rates of Anopheles spp. in both
forested and deforested study sites
8/18/2015
UNCLASSIFIED
9
www.afrims.org
Study area
• Anlong Veng district,
Cambodia
• Study sites
- Deforested area
(Trapeangtrav Health
Center)
- Forested areas
(Chhomk Ron and
Keam Ra Meas
military camp)
8/18/2015
UNCLASSIFIED
10
www.afrims.org
Mosquito trapping
• CO2-baited CDC light trap.
• Standard trap setup using 1
kilogram of dry-ice.
• 10 traps per field site.
• Mosquitoes were separated to
genera and all Anopheles were
morphologically identified to species.
• All samples were stored on dry-ice
until returned to Bangkok when they
were frozen at -80ºC.
8/18/2015
UNCLASSIFIED
11
www.afrims.org
Malaria detection
•
Malaria parasite detections in primary and secondary malaria
vectors were conducted using PCR technique.
•
Primary malaria vector (An. dirus, An. minimus and An. maculatus)
were individually processed using PCR.
•
Secondary malaria vector and potential malaria vector were pooled
20 samples per reaction for PCR.
•
Each pooled mosquito samples were sorted by species, month,
and location.
8/18/2015
UNCLASSIFIED
12
www.afrims.org
Results
Total captured mosquitoes
(Dec 2012 - Sep 2013)
40000
35000
30000
Other spp. (Ar., Cq.,
Ad., Ur.)
25000
• 8 mosquito genera represented
•Aedeomyia, Aedes, Anopheles,
Armigeres, Coquillettidia, Culex,
Mansonia and Uranotaenia spp.
Aedes
20000
Mansonia
Culex
15000
Anopheles
10000
•Total 29 Anopheles spp.
• Health Center (HC) 23 species
• Military camp (K4) 25 species
• Military camp (K6) 29 species
5000
0
HC
8/18/2015
K4
K6
UNCLASSIFIED
13
www.afrims.org
Results: Total number of collected Anopheles mosquitoes
2100
2000
1900
1800
1700
1600
1500
1400
1300
1200
1100
1000
900
800
700
600
500
400
300
200
100
0
2000
1419
Health Center (HC)
Military Camp (K4)
Military Camp (K6)
794
386
279
270
194
127
80
Dec-12
8/18/2015
182
79
4
Jan-13
6
Feb-13
145
18
Mar-13
404
341
166
129
105
76
51
6
Apr-13
May-13
Jun-13
728
312
49
Jul-13
79
55
63
61
Aug-13
Sep-13
UNCLASSIFIED
14
www.afrims.org
Results: Malaria parasite detection
Species
Malaria vector
34
An. dirus
An. maculatus
# mosquitoes
processed for
malaria PCR
detection
primary
• Anopheles vectors tested all “malaria NEGATIVE”
15
46
An. minimus
26
An. aconitus
secondary
48
An. sawadwongporni
An. annularis
186
An. barbirostris
539
8
An. campestris
potential
An. philippinensis
227
An. pseudojamesi
301
An. sinensis
8/18/2015
14
UNCLASSIFIED
15
www.afrims.org
Field evaluation of spatial repellents and attractants to
develop an integrated push-pull system for Aedes
aegypti control
Objective
To develop an integrated push-pull system for the control of Ae. aegypti.
•
Attractants were evaluated for their efficacy in pulling biting flies away
from potential hosts and into traps where they are killed.
•
Spatial repellents were evaluated for their efficacy in pushing biting flies
away from potential hosts.
8/18/2015
UNCLASSIFIED
16
www.afrims.org
Experimental Tunnel (50 m.)
1.5 m
50 m
1.0 m
60x60x60 cm
Interception trap
8/18/2015
Inside the tunnel
UNCLASSIFIED
17
17
www.afrims.org
Experiment Trials in 50 m Tunnels
Mosquitoes: Aedes aegypti / Anopheles dirus (AFRIMS strain)
Daytime: 08.00 AM - 04.00 PM / 06.00 PM - 01.00 AM
100 females / tunnel
1.5 m
1.9 m
Attractant trails
Treatment
Control
Repellent trails
Interception trap
8/18/2015
Attractant device
Mosquito
releasing point
Repellent device
UNCLASSIFIED
18
18
www.afrims.org
Attractants were placed in
the middle of the tent.
8/18/2015
UNCLASSIFIED
Repellents were placed in
front of the tent window/
interceptionwww.themegallery.com
trap.
19
19
www.afrims.org
Repellents and Attractants
ThermaCELL®, Lantern
(d-cis/trans allethrin)
Mosquito Cognito®
(linalool)
Mega-Catch Fragrance Strip
(1-octen-3-ol)
Hot Shot® No-Pest® Strip
(dichlovos)
Mosquito Magnet® Octenol
(1-octen-3-ol)
Bug Band® bead bag
(geraniol)
Mosquito Magnet® Lurex3
(lactic acid)
Mosquito Grid Bug Bam
(citronella oil, geranium oil,
lemongrass oil, peppermint oil)
8/18/2015
UNCLASSIFIED
20
20
www.afrims.org
Push-Pull system
Tested products
Name
Type
Active ingredient(s)
Attractant
1-octen-3-ol (24.5%)
Attractant
1-octen-3-ol (55.15%)
Mosquito Magnet® Lurex3™
Attractant
lactic acid (35.4%)
ThermaCELL®
Repellent
d-cis/trans allethrin (21.97%)
Bug Band® bead bag
Repellent
geraniol (20%)
Mosquito Grid® bug bam
Repellent
citronella oil (1%), geranium oil (7%), lemongrass oil (4%), peppermint oil(3%)
Mosquito Cognito®
Repellent
Linalool (95.54%)
Hot Shot™ No-Pest® Strip
Repellent
Dichlovos (18.6%)
*8/18/2015
All devices are registered and approved by EPA
UNCLASSIFIED
21
21
www.afrims.org
Estimated mean ±SE of proportion of recaptured
Ae. aegypti
Repellent product
p < 0.05
0.6
Proportion of recaptured mosquito
Attractant product
0.6
p < 0.05
0.5
0.5
0.4
0.4
0.3
0.3
0.2
0.2
0.1
0.1
0
0
ThermaCELL
Bug Band
Mosquito
Grid
Control
8/18/2015
Mosquito
Cognito
Hot Shot
Treatment
Mega-Catch 3000
Mosquito Magnet
Octenol
Control
Mosquito Magnet
Lurex3
Treatment
UNCLASSIFIED
22
www.afrims.org
Estimated mean ±SE of proportion of recaptured
An. dirus
Proportion of recaptured mosquito
Repellent product
Attractant product
0.5
0.5
0.4
0.4
0.3
0.3
0.2
0.2
0.1
0.1
0
0
ThermaCELL
Bug Band
Mosquito
Grid
Control
8/18/2015
Mosquito
Cognito
Hot Shot
Treatment
Mega-Catch 3000
Mosquito Magnet
Octenol
Control
Mosquito Magnet
Lurex3
Treatment
UNCLASSIFIED
23
www.afrims.org
Conclusions
Name
Type
Active ingredient(s)
Attractant
1-octen-3-ol (24.5%)
Attractant
1-octen-3-ol (55.15%)
Mosquito Magnet® Lurex3™
Attractant
lactic acid (35.4%)
•
•
All three devices were attractive as “human bait”.
The commercial attractants can be used in regular
dengue/malaria vector surveillance program.
ThermaCELL®
Repellent
d-cis/trans allethrin (21.97%)
•
ThermaCell has been tested and proved as an effective spatial
repellent against Ae. aegypti under the semi-field conditions in
Thailand.
8/18/2015
24
UNCLASSIFIED
24
www.afrims.org
CO2 generator evaluation
Field evaluation of CO2 generators in Thailand
Objective:
To evaluate the efficacy of CO2
generators under field conditions in
malaria transmission areas.
Study sites: Mae Sot, Tak province
8/18/2015
Armed Forces
Institute
of Medical
SciencesSciences
Armed
ForcesResearch
Research
Institute
of Medical
UNCLASSIFIED
25
25
www.afrims.org
www.afrims.org
TDA Research Generation 5 Prototype
(TDA Research, Inc, CO, USA)
8/18/2015
Armed Forces
Institute
of Medical
SciencesSciences
Armed
ForcesResearch
Research
Institute
of Medical
UNCLASSIFIED
26
26
www.afrims.org
www.afrims.org
CUBE CO2 Generator Unit
(CUBE Technology Inc, AZ, USA)
8/18/2015
Armed Forces
Institute
of Medical
SciencesSciences
Armed
ForcesResearch
Research
Institute
of Medical
UNCLASSIFIED
27
27
www.afrims.org
www.afrims.org
MOUSTIQ-AIRTM CO2 Generator
(Med-e-Cell company, CA, USA)
8/18/2015
Armed Forces
Institute
of Medical
SciencesSciences
Armed
ForcesResearch
Research
Institute
of Medical
UNCLASSIFIED
28
28
www.afrims.org
www.afrims.org
Field Evaluation of Treated Barriers
8/18/2015
UNCLASSIFIED
29
www.afrims.org
Field Evaluation of CHIKV Dipsticks
8/18/2015
UNCLASSIFIED
30
www.afrims.org
Wolbachia-mediated interference of dengue virus
transmission
8/18/2015
UNCLASSIFIED
31
www.afrims.org
Experiments in large mosquito enclosures
(LME)
LMEs environment
8/18/2015
UNCLASSIFIED
32
www.afrims.org
Push-pull experiment
8/18/2015
UNCLASSIFIED
33
www.afrims.org
SkySight™ Tent Trap Evaluation
8/18/2015
UNCLASSIFIED
34
www.afrims.org
Pyriproxyfen/Spinosad treated device evaluation
8/18/2015
UNCLASSIFIED
35
www.afrims.org
Training
8/18/2015
UNCLASSIFIED
36
www.afrims.org
Thank you.
8/18/2015
UNCLASSIFIED
37
37
www.afrims.org

unclassified

Transcription

Similar documents