Mascot Search Results

Transcription

Peptide Summary Report (N_18_7_09_1.RAW relex2)
Figure S1 Peptide summary reports for LTQ FT-mass spectrometry
searches against the NCBInr data base (July, 2009).
Page 1 of 18
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MS data file
Database
Timestamp
Protein hits
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LTQFT Daemon
N_18_7_09_1.RAW relex2
D:\Data\Mark\18_7-09\N_18_7_09_1.RAW
NCBInr 4_9_09 (9636254 sequences; 3294494089 residues)
9 Dec 2009 at 03:54:10 GMT
gi|11935049 keratin 1 [Homo sapiens]
gi|28317
unnamed protein product [Homo sapiens]
gi|114667176 PREDICTED: similar to Keratin, type I cytoskeletal 14 (Cytokeratin-14) (CK-14) (Keratin-14) (K14) [Pan troglodytes]
gi|2136760
glycoprotein antigen MGP57/53, mammary gland - bovine (fragment)
gi|146741296 keratin 1 [Sus scrofa]
gi|9725
unnamed protein product [Manduca sexta]
gi|157165473 sodium-dependent tyrosine transporter [Campylobacter concisus 13826]
gi|6729922
Chain A, Nmr Study Of Bovine Heart Fatty Acid Binding Protein
gi|229552
albumin
gi|15673843 elongation factor Tu [Lactococcus lactis subsp. lactis Il1403]
gi|181402
epidermal cytokeratin 2 [Homo sapiens]
gi|136429
RecName: Full=Trypsin; Flags: Precursor
gi|123506
RecName: Full=Hydrophobic seed protein; Short=HPS; AltName: Allergen=Gly m 1
gi|2266432
butyrophilin [Bos taurus]
gi|2851513
RecName: Full=Lactadherin; AltName: Full=Milk fat globule-EGF factor 8; Short=MFG-E8; AltName: Full=MFGM; AltName: Full=Sper
gi|3914346
RecName: Full=Polymeric immunoglobulin receptor; Short=Poly-Ig receptor; Short=PIGR; Contains: RecName: Full=Secretory compo
gi|83764016 prepro complement component C3 [Bos taurus]
gi|62126072 endopin 2C [Bos taurus]
gi|6680604
keratin 16 [Mus musculus]
gi|15672820 30S ribosomal protein S1 [Lactococcus lactis subsp. lactis Il1403]
gi|77736341 serpin peptidase inhibitor, clade C (antithrombin), member 1 [Bos taurus]
gi|223165
lactoglobulin beta
gi|15900538 50S ribosomal protein L1 [Streptococcus pneumoniae TIGR4]
gi|2232299
IgM heavy chain constant region [Bos taurus]
gi|28592070 IgM heavy chain constant region, secretory form [Bos taurus]
gi|6320157
Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 di
gi|538275
alpha1-antichymotrypsin isoform pHHK11
gi|149458033 PREDICTED: similar to chymotrypsin C (caldecrin), partial [Ornithorhynchus anatinus]
gi|4927746
RpS3 [Streptococcus pneumoniae]
gi|15672484 HU like DNA-binding protein [Lactococcus lactis subsp. lactis Il1403]
NCBInr Decoy False discovery rate
Peptide matches above identity threshold
53
1
1.89 %
Peptide matches above homology or identity threshold
76
1
1.32 %
Probability Based Mowse Score
Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 49 indicate identity or extensive homology (p<0.01).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.
Peptide Summary Report
Format As
Max. number of hits AUTO
Standard scoring
Ions score or expect cut-off 1
Show sub-sets 0
Sort unassigned Decreasing Score
Require bold red
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1.
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Peptide Summary
Significance threshold p< 0.01
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MudPIT scoring
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gi|11935049
Mass: 66027
keratin 1 [Homo sapiens]
Error tolerant
Score: 591
Archive Report
Queries matched: 14
emPAI: 0.47
Check to include this hit in error tolerant search or archive report
Query
41
64
76
Observed
437.7532
487.2691
500.2263
Mr(expt)
873.4918
972.5236
998.4380
Mr(calc)
873.4920
972.5240
998.4379
ppm Miss Score
-0.14
0
27
-0.33
0
43
0.15
0
17
Expect Rank
5.4
8
0.099
1
10
2
Peptide
R.SLVNLGGSK.S
K.IEISELNR.V
K.DVDGAYMTK.V
http://mascotx/mascot/cgi/master_results.pl?file=..%2Fdata%2F20091209%2FF067494.dat&REPTY...
12/9/2009
95
106
167
198
216
221
229
233
234
249
301
517.2615
533.2642
590.3035
633.3221
651.8607
670.8381
692.3486
697.3697
465.2490
738.3959
858.9290
1032.5084
1064.5138
1178.5924
1264.6296
1301.7068
1339.6616
1382.6826
1392.7248
1392.7252
1474.7772
1715.8434
1032.5087
1064.5138
1178.5931
1264.6299
1301.7078
1339.6619
1382.6830
1392.7249
1392.7249
1474.7780
1715.8438
-0.27
0.04
-0.57
-0.21
-0.75
-0.20
-0.26
-0.02
0.22
-0.50
-0.21
Proteins matching the same set of peptides:
Mass: 65999
Score: 591
gi|119395750
gi|160961491
Mass: 65450
Score: 591
keratin 1 [Pan troglodytes]
2.
gi|28317
Mass: 59492
Score: 584
0
0
0
0
0
1
0
1
1
0
0
34
0.41
21
13
72 8.3e-005
39
0.21
57
0.0016
42
0.061
65
0.00038
59
0.0012
(9) 1.3e+002
52
0.0078
64
0.0005
1
1
1
1
1
1
1
1
5
1
1
Page 2 of 18
R.TLLEGEESR.M
K.AQYEDIAQK.S
K.YEELQITAGR.H
R.TNAENEFVTIK.K
R.SLDLDSIIAEVK.A
K.SKAEAESLYQSK.Y
K.SLNNQFASFIDK.V
R.TNAENEFVTIKK.D
R.TNAENEFVTIKK.D
R.FLEQQNQVLQTK.W
K.QISNLQQSISDAEQR.G
Queries matched: 14
Queries matched: 14
Queries matched: 16
emPAI: 0.38
Query
13
93
Observed
404.2033
516.3027
Mr(expt)
806.3920
1030.5908
Mr(calc)
806.3923
1030.5910
ppm Miss Score
-0.26
0
41
-0.17
0
41
105
121
159
177
185
197
223
224
226
228
230
251
252
299
532.8083
545.7689
583.2961
601.3116
617.8427
631.8021
453.2442
679.3630
683.3234
691.3276
695.8439
498.5828
747.3706
854.3896
1063.6020
1089.5232
1164.5776
1200.6086
1233.6708
1261.5896
1356.7108
1356.7114
1364.6322
1380.6406
1389.6732
1492.7266
1492.7266
1706.7646
1063.6026
1089.5237
1164.5775
1200.6098
1233.6717
1261.5899
1356.7110
1356.7110
1364.6320
1380.6408
1389.6736
1492.7270
1492.7270
1706.7649
-0.49
-0.40
0.15
-1.00
-0.68
-0.17
-0.13
0.36
0.17
-0.14
-0.22
-0.27
-0.22
-0.15
1
0
0
0
1
0
1
1
0
0
0
1
1
0
13
59
39
34
25
69
14
(6)
32
73
39
(7)
40
65
Expect Rank
0.086
1
0.045
1
35
0.0013
0.18
0.38
3.2
9.7e-005
59
3.8e+002
0.57
6.1e-005
0.13
2.2e+002
0.11
0.0002
1
1
1
1
1
1
1
10
1
1
1
2
1
1
Peptide
R.LAADDFR.L
R.VLDELTLTK.A
R.LASYLDKVR.A
K.VTMQNLNDR.L
R.LENEIQTYR.S
R.QSVEADINGLR.R
R.LKYENEVALR.Q
R.SLLEGEGSSGGGGR.G
R.QSVEADINGLRR.V
R.QSVEADINGLRR.V
R.SQYEQLAEQNR.K
R.ALEESNYELEGK.I
K.QSLEASLAETEGR.Y
R.SQYEQLAEQNRK.D
R.SQYEQLAEQNRK.D
K.GSLGGGFSSGGFSGGSFSR.G
gi|21961605
Mass: 58792
Score: 584
Queries matched: 16
Keratin 10 [Homo sapiens]
Mass: 62761
Score: 584
Queries matched: 16
gi|114667509
PREDICTED: similar to keratin 10 isoform 1 [Pan troglodytes]
gi|114667511
Mass: 58244
Score: 584
Queries matched: 16
Mass: 56692
Score: 584
Queries matched: 16
gi|114667513
Mass: 63308
Score: 584
Queries matched: 16
gi|119581085
keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), isoform CRA_b [Homo sapiens]
gi|195972866
Mass: 58766
Score: 584
Queries matched: 16
Mass: 58818
Score: 584
Queries matched: 16
gi|251757513
RecName: Full=Keratin, type I cytoskeletal 10; AltName: Full=Cytokeratin-10; Short=CK-10; AltName: Full=Keratin-10; Short=K10
Mass: 100282
Score: 581
Queries matched: 16
gi|114667507
PREDICTED: keratin 25D isoform 4 [Pan troglodytes]
3.
gi|114667176
Mass: 107386
Score: 381
Queries matched: 7
emPAI: 0.16
PREDICTED: similar to Keratin, type I cytoskeletal 14 (Cytokeratin-14) (CK-14) (Keratin-14) (K14) [Pan troglodytes]
Query
13
105
121
153
183
187
313
4.
Observed
404.2033
532.8083
545.7689
579.2987
616.8022
618.2680
896.3679
Mr(expt)
806.3920
1063.6020
1089.5232
1156.5828
1231.5898
1234.5214
1790.7212
Mr(calc)
806.3923
1063.6026
1089.5237
1156.5836
1231.5906
1234.5215
1790.7205
ppm Miss Score
Expect Rank
-0.26
0
41
0.086
1
-0.49
1
13
35
1
-0.40
0
59
0.0013
1
-0.69
0
52
0.0039
1
-0.58
0
86 2.9e-006
1
-0.00
0
69
4e-005
1
0.42
0
61 9.8e-005
1
Peptide
R.LAADDFR.T
R.LASYLDKVR.A
K.VTMQNLNDR.L
R.QGVDADINGLR.Q
R.SGGGGGGGLGSGGSIR.S
R.FSSSSGYGGGSSR.V
R.GGSGGSYGGGGSGGGYGGGSGSR.G
gi|2136760
Mass: 44735
Score: 328
Queries matched: 16
emPAI: 0.43
Query
19
Observed
419.7186
Mr(expt)
837.4226
Mr(calc)
837.4232
ppm Miss Score
-0.66
0
26
Expect Rank
3.2
1
Peptide
R.AGSAEYLK.T
12/9/2009
20
35
86
101
174
175
232
236
239
240
241
242
243
244
326
419.7188
434.7115
508.2982
530.7688
597.3246
597.3247
696.3583
704.3563
480.5734
720.3566
480.5736
720.3568
480.5737
720.3577
681.6852
837.4230
867.4084
1014.5818
1059.5230
1192.6346
1192.6348
1390.7020
1406.6980
1438.6984
1438.6986
1438.6990
1438.6990
1438.6993
1438.7008
2042.0338
837.4232
867.4086
1014.5822
1059.5237
1192.6353
1192.6353
1390.7027
1406.6977
1438.6993
1438.6993
1438.6993
1438.6993
1438.6993
1438.6993
2042.0334
-0.19
-0.23
-0.35
-0.57
-0.55
-0.38
-0.50
0.27
-0.67
-0.48
-0.26
-0.20
-0.05
1.05
0.19
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
(24)
5.6
35
0.18
66
0.0003
9 1.7e+002
45
0.04
(43)
0.072
72 7.2e-005
(61) 0.00086
(22)
5.7
(51)
0.0074
(32)
0.61
(51)
0.0072
(30)
1
53
0.005
21
8.1
6
1
1
3
1
1
1
1
1
1
1
1
1
1
1
Page 3 of 18
R.AGSAEYLK.T
K.VAYSTDGR.Q
R.VTGIITQGAR.D
K.QITASSYYK.T
R.QFQFIQVAGR.S
R.QFQFIQVAGR.S
K.MWVTGVVTQGASR.A
K.MWVTGVVTQGASR.A + Oxidation (M)
R.DFGHIQYVAAYR.V
R.DFGHIQYVAAYR.V
R.DFGHIQYVAAYR.V
R.DFGHIQYVAAYR.V
R.DFGHIQYVAAYR.V
R.DFGHIQYVAAYR.V
K.VAYSTDGRQFQFIQVAGR.S
gi|2494285
Mass: 47380
Score: 328
Queries matched: 16
RecName: Full=Lactadherin; AltName: Full=Milk fat globule-EGF factor 8; Short=MFG-E8; AltName: Full=MGP57/53; AltName: Full=PAS-6/PAS-7 glycoprotein;
gi|2652932
Mass: 45502
Score: 328
Queries matched: 16
bP47 protein [Bos taurus]
Mass: 47471
Score: 328
Queries matched: 16
gi|41386719
milk fat globule-EGF factor 8 protein [Bos taurus]
gi|74354284
Mass: 47831
Score: 328
Queries matched: 16
MFGE8 protein [Bos taurus]
5.
gi|146741296
Mass: 65160
keratin 1 [Sus scrofa]
Score: 268
Queries matched: 6
emPAI: 0.10
Query
41
64
95
124
229
249
6.
Observed
437.7532
487.2691
517.2615
554.2750
692.3486
738.3959
Mr(expt)
873.4918
972.5236
1032.5084
1106.5354
1382.6826
1474.7772
Mr(calc)
873.4920
972.5240
1032.5087
1106.5356
1382.6830
1474.7780
ppm Miss Score Expect Rank
-0.14
0
27
5.4
8
-0.33
0
43
0.099
1
-0.27
0
34
0.41
1
-0.14
0
48
0.027
1
-0.26
0
65 0.00038
1
-0.50
0
52
0.0078
1
gi|9725
Mass: 17837
Score: 263
Queries matched: 19
Peptide
R.SLVNLGGSK.S
K.IEISELNR.V
R.TLLEGEESR.M
K.AQYEEIAQR.S
K.SLNNQFASFIDK.V
R.FLEQQNQVLQTK.W
emPAI: 0.68
Query
6
109
110
116
117
254
255
256
257
258
259
260
261
307
308
309
310
311
312
Observed
383.2198
534.3137
534.3138
541.2797
541.2799
762.3937
762.3939
508.5984
508.5984
762.3942
762.3942
762.3945
762.3945
890.4462
890.4465
890.4472
894.4670
894.4673
894.4673
Mr(expt)
764.4250
1066.6128
1066.6130
1080.5448
1080.5452
1522.7728
1522.7732
1522.7734
1522.7734
1522.7738
1522.7738
1522.7744
1522.7744
1778.8778
1778.8784
1778.8798
1786.9194
1786.9200
1786.9200
Mr(calc)
764.4255
1066.6135
1066.6135
1080.5451
1080.5451
1522.7740
1522.7740
1522.7740
1522.7740
1522.7740
1522.7740
1522.7740
1522.7740
1778.8799
1778.8799
1778.8799
1786.9200
1786.9200
1786.9200
ppm Miss Score
Expect Rank
-0.56
0
28
0.68
6
-0.59
0
(23)
2.6
1
-0.41
0
35
0.17
1
-0.25
0
54
0.0041
1
0.12
0
(23)
6
1
-0.73
1
(25)
4.1
1
-0.46
1
(33)
0.67
1
-0.38
1
(25)
4.2
1
-0.38
1
(13)
68
5
-0.07
1
56
0.0027
1
-0.07
1
(31)
1
1
0.32
1
(40)
0.11
1
0.32
1
(25)
3.4
1
-1.14
1
(13)
69
1
-0.80
1
(5) 4.6e+002
2
-0.01
1
30
1.4
2
-0.32
0
(22)
7.9
1
0.02
0
59
0.0015
1
0.02
0
(22)
8.8
1
Peptide
-.MQIFVK.T
K.ESTLHLVLR.L
K.ESTLHLVLR.L
R.TLSDYNIQK.E
R.TLSDYNIQK.E
K.IQDKEGIPPDQQR.L
K.IQDKEGIPPDQQR.L
K.IQDKEGIPPDQQR.L
K.IQDKEGIPPDQQR.L
K.IQDKEGIPPDQQR.L
K.IQDKEGIPPDQQR.L
K.IQDKEGIPPDQQR.L
K.IQDKEGIPPDQQR.L
K.QLEDGRTLSDYNIQK.E
K.QLEDGRTLSDYNIQK.E
K.QLEDGRTLSDYNIQK.E
K.TITLEVEPSDTIENVK.A
gi|158769
Mass: 8428
Score: 263
Queries matched: 19
ubiquitin
gi|158771
Mass: 8543
Score: 263
Queries matched: 19
ubiquitin
Mass: 8446
Score: 263
Queries matched: 19
gi|229532
ubiquitin
gi|600539
Mass: 18117
Score: 263
Queries matched: 19
ubiquitin [synthetic construct]
gi|1050930
Mass: 11234
Score: 263
Queries matched: 19
polyubiquitin [Rattus norvegicus]
gi|1167510
Mass: 14167
Score: 263
Queries matched: 19
TI-225 [Mus musculus]
gi|1421797
Mass: 8932
Score: 263
Queries matched: 19
polyubiquitin
12/9/2009
Page 4 of 18
gi|2149467
Mass: 558548
Score: 263
Queries matched: 19
predicted to encode 4983 amino acids with a predicted molecular mass of 558 kDa; ILLC-BVDV ORF [pestivirus type 1]
gi|4506713
Mass: 17953
Score: 263
Queries matched: 19
ubiquitin and ribosomal protein S27a precursor [Homo sapiens]
gi|4507761
Mass: 14719
Score: 263
Queries matched: 19
ubiquitin and ribosomal protein L40 precursor [Homo sapiens]
gi|5822011
Mass: 8614
Score: 263
Queries matched: 19
Chain B, Structural Basis For The Specificity Of Ubiquitin C- Terminal Hydrolases
gi|11528469
Mass: 64092
Score: 263
Queries matched: 19
humanized L1/ubiqutin hybrid protein [synthetic construct]
gi|12240039
Mass: 14664
Score: 263
Queries matched: 19
ubiquitin [Biomphalaria glabrata]
gi|13021896
Mass: 62384
Score: 263
Queries matched: 19
humanized ubiquitin/L1 delta/H-2 Db CTL epitope hybrid protein [synthetic construct]
gi|13172895
Mass: 8673
Score: 263
Queries matched: 19
polyubiquitin GmUblast [Galleria mellonella]
Mass: 17939
Score: 263
Queries matched: 19
gi|13195690
ribosomal protein S27a [Mus musculus]
gi|15187109
Mass: 14738
Score: 263
Queries matched: 19
ubiquitin [Branchiostoma belcheri]
gi|15293953
Mass: 14775
Score: 263
Queries matched: 19
ribosomal protein L40 [Ictalurus punctatus]
Mass: 14720
Score: 263
Queries matched: 19
gi|17136570
ribosomal protein L40 [Drosophila melanogaster]
gi|17136574
Mass: 17929
Score: 263
Queries matched: 19
ribosomal protein S27A [Drosophila melanogaster]
gi|18071662
Mass: 17995
Score: 263
Queries matched: 19
ubiquitin/ribosomal protein S27a fusion protein [Branchiostoma belcheri tsingtaunese]
gi|18253057
Mass: 17962
Score: 263
Queries matched: 19
ribosomal protein S27A [Spodoptera frugiperda]
gi|28189426
Mass: 16603
Score: 263
Queries matched: 19
similar to ubiquitin-S27a fusion protein [Bos taurus]
gi|29612581
Mass: 19698
Score: 263
Queries matched: 19
Zgc:66168 protein [Danio rerio]
gi|39840928
Mass: 17853
Score: 263
Queries matched: 19
ubiquitin [Antheraea yamamai]
gi|41055516
Mass: 17988
Score: 263
Queries matched: 19
ribosomal protein S27a [Danio rerio]
gi|47217408
Mass: 14736
Score: 263
Queries matched: 19
unnamed protein product [Tetraodon nigroviridis]
Mass: 17968
Score: 263
Queries matched: 19
gi|47604951
ribosomal protein S27a [Gallus gallus]
Mass: 8540
Score: 263
Queries matched: 19
gi|48425521
Chain B, Tsg101(Uev) Domain In Complex With Ubiquitin
gi|49532928
Mass: 17936
Score: 263
Queries matched: 19
Ribosomal protein S27A [Plutella xylostella]
gi|50344522
Mass: 17838
Score: 263
Queries matched: 19
ubiquitin/S27Ae ribosomal protein [Carabus granulatus]
gi|51701909
Mass: 8578
Score: 263
Queries matched: 19
RecName: Full=Ubiquitin
gi|51701919
Mass: 8560
Score: 263
Queries matched: 19
Mass: 14739
Score: 263
Queries matched: 19
gi|55228560
ubuiquitin/ribosomal L40 fusion protein [Scleronephthya gracillimum]
gi|56078799
Mass: 17893
Score: 263
Queries matched: 19
Ribosomal protein S27a [Homo sapiens]
gi|56967061
Mass: 8503
Score: 263
Queries matched: 19
Chain B, Crystal Structure Of Uchl3-Ubvme Complex
gi|61741081
Mass: 16173
Score: 263
Queries matched: 19
ubiquitin/ribosomal 27a [Marsupenaeus japonicus]
Mass: 11036
Score: 263
Queries matched: 19
gi|66361226
Chain B, Solution Structure Of S5a Uim-1UBIQUITIN COMPLEX
gi|66362872
Mass: 15100
Score: 263
Queries matched: 19
60S ribosomal protein L40 [Cryptosporidium parvum Iowa II]
gi|67083981
Mass: 17924
Score: 263
Queries matched: 19
ubiquitin/ribosomal protein S27a fusion protein [Ixodes scapularis]
gi|67614873
Mass: 14658
Score: 263
Queries matched: 19
ubiquitin / ribosomal protein CEP52 [Cryptosporidium hominis TU502]
gi|69608566
Mass: 17951
Score: 263
Queries matched: 19
ubiquitin/ribosomal protein S27Ae fusion protein [Agriotes lineatus]
gi|69608583
Mass: 17879
Score: 263
Queries matched: 19
ubiquitin/ribosomal protein S27Ae fusion protein [Eucinetus sp. APV-2005]
gi|69608591
Mass: 17880
Score: 263
Queries matched: 19
ubiquitin/ribosomal protein S27Ae fusion protein [Timarcha balearica]
gi|70909907
Mass: 14693
Score: 263
Queries matched: 19
ribosomal protein Ubq/L40e [Agriotes lineatus]
gi|70909913
Mass: 14679
Score: 263
Queries matched: 19
ribosomal protein Ubq/L40e [Dascillus cervinus]
gi|71033899
Mass: 14887
Score: 263
Queries matched: 19
ubiquitin/ribosomal fusion protein [Theileria parva strain Muguga]
gi|73970166
Mass: 26177
Score: 263
Queries matched: 19
12/9/2009
Page 5 of 18
PREDICTED: similar to ubiquitin and ribosomal protein S27a precursor [Canis familiaris]
gi|80751117
Mass: 14702
Score: 263
Queries matched: 19
hypothetical protein LOC612529 [Canis lupus familiaris]
gi|80751129
Mass: 14747
Score: 263
Queries matched: 19
ubiquitin A-52 residue ribosomal protein fusion product 1 [Danio rerio]
gi|84997964
Mass: 14869
Score: 263
Queries matched: 19
ubiquitin/60S ribosomal fusion protein [Theileria annulata]
gi|85719967
Mass: 12889
Score: 263
Queries matched: 19
ubiquitin and ribosomal protein S27a precursor [Ictalurus punctatus]
gi|90819968
Mass: 17864
Score: 263
Queries matched: 19
putative ubiquitin/ribosomal protein S27Ae fusion protein [Graphocephala atropunctata]
gi|91077776
Mass: 17822
Score: 263
Queries matched: 19
PREDICTED: similar to ubiquitin/S27Ae ribosomal protein [Tribolium castaneum]
gi|91080141
Mass: 14750
Score: 263
Queries matched: 19
PREDICTED: similar to ribosomal protein Ubq/L40e [Tribolium castaneum]
gi|94369204
Mass: 8723
Score: 263
Queries matched: 19
PREDICTED: hypothetical protein [Mus musculus]
Mass: 14763
Score: 263
Queries matched: 19
gi|109121596
PREDICTED: similar to ubiquitin A-52 residue ribosomal protein fusion product 1 [Macaca mulatta]
gi|109157803
Mass: 9016
Score: 263
Queries matched: 19
Chain A, Crystal Structure Of The 9-10 8 Glycine Insertion Mutant Of Ubiquitin.
gi|109157805
Mass: 9341
Score: 263
Queries matched: 19
Chain A, Crystal Structure Of The 9-10 Moad Insertion Mutant Of Ubiquitin
Mass: 14795
Score: 263
Queries matched: 19
gi|110671470
putative ubiquitin/ribosomal fusion protein [Diaphorina citri]
gi|113431898
Mass: 15448
Score: 263
Queries matched: 19
ubiquitin/ribosomal fusion protein homologue [Salmo salar]
gi|114577583
Mass: 25695
Score: 263
Queries matched: 19
PREDICTED: similar to RPS27A protein [Pan troglodytes]
gi|114662918
Mass: 17926
Score: 263
Queries matched: 19
PREDICTED: similar to ubiquitin isoform 1 [Pan troglodytes]
gi|119624908
Mass: 8663
Score: 263
Queries matched: 19
ubiquitin B, isoform CRA_b [Homo sapiens]
gi|119624910
Mass: 13066
Score: 263
Queries matched: 19
ubiquitin B, isoform CRA_d [Homo sapiens]
gi|119909248
Mass: 8629
Score: 263
Queries matched: 19
PREDICTED: similar to ubiquitin B [Bos taurus]
gi|121543853
Mass: 17854
Score: 263
Queries matched: 19
putative ubiquitin/ribosomal protein S27Ae fusion protein [Maconellicoccus hirsutus]
gi|123316118
Mass: 17772
Score: 263
Queries matched: 19
ubiquitin [Portunus pelagicus]
Mass: 14733
Score: 263
Queries matched: 19
gi|124300855
ubiquitin [Solea senegalensis]
Mass: 17875
Score: 263
Queries matched: 19
gi|125742521
putative ubiquitin/ribosomal protein S27Ae fusion protein [Oncopeltus fasciatus]
gi|148688761
Mass: 10235
Score: 263
Queries matched: 19
mCG1048340 [Mus musculus]
gi|148708840
Mass: 14662
Score: 263
Queries matched: 19
gi|149044848
Mass: 16414
Score: 263
Queries matched: 19
rCG23287, isoform CRA_b [Rattus norvegicus]
gi|149287148
Mass: 17968
Score: 263
Queries matched: 19
ubiquitin/40S ribosomal protein S27a [Ornithodoros parkeri]
Mass: 14794
Score: 263
Queries matched: 19
gi|152013697
ubiquitin/ribosomal L40 fusion protein [Artemia franciscana]
gi|152925970
Mass: 14702
Score: 263
Queries matched: 19
ubiquitin fusion protein [Cyprinus carpio]
gi|156083999
Mass: 14775
Score: 263
Queries matched: 19
ubiquitin / ribosomal protein CEP52 [Babesia bovis T2Bo]
gi|156396888
Mass: 16792
Score: 263
Queries matched: 19
predicted protein [Nematostella vectensis]
Mass: 17968
Score: 263
Queries matched: 19
gi|156405092
gi|157136377
Mass: 17986
Score: 263
Queries matched: 19
ubiquitin (ribosomal protein L40) [Aedes aegypti]
gi|157361519
Mass: 14694
Score: 263
Queries matched: 19
ubiquitin/ribosomal L40 fusion protein-like protein [Phlebotomus papatasi]
gi|159145744
Mass: 14669
Score: 263
Queries matched: 19
putative ribosomal protein L40 [Barentsia elongata]
gi|159163685
Mass: 8588
Score: 263
Queries matched: 19
Chain B, Solution Structure Of The Human Mms2-Ubiquitin Complex
gi|161661039
Mass: 17995
Score: 263
Queries matched: 19
60S ribosomal protein L40A [Lycosa singoriensis]
gi|169146693
Mass: 9909
Score: 263
Queries matched: 19
novel protein [Danio rerio]
gi|185134441
Mass: 14733
Score: 263
Queries matched: 19
ubiquitin [Oncorhynchus mykiss]
gi|188572494
Mass: 17982
Score: 263
Queries matched: 19
putative ubiquitin/40S ribosomal protein RPS27A fusion protein [Phoronis muelleri]
gi|194246105
Mass: 17904
Score: 263
Queries matched: 19
ubiquitin/ribosomal protein S27a fusion protein [Dermacentor variabilis]
gi|195116685
Mass: 17915
Score: 263
Queries matched: 19
12/9/2009
Page 6 of 18
GI10618 [Drosophila mojavensis]
gi|195190296
Mass: 8833
Score: 263
Queries matched: 19
GL14086 [Drosophila persimilis]
gi|195342419
Mass: 14707
Score: 263
Queries matched: 19
GM18458 [Drosophila sechellia]
gi|195491526
Mass: 8872
Score: 263
Queries matched: 19
GE20668 [Drosophila yakuba]
gi|195565407
Mass: 11649
Score: 263
Queries matched: 19
GD16200 [Drosophila simulans]
gi|197107121
Mass: 8606
Score: 263
Queries matched: 19
Chain B, Cin85 Sh3-C Domain In Complex With Ubiquitin
gi|197632563
Mass: 18002
Score: 263
Queries matched: 19
ribosomal protein S27-3 [Salmo salar]
gi|197725013
Mass: 8675
Score: 263
Queries matched: 19
Chain C, Crystal Structure Of Human Amsh-Lp Dub Domain In Complex With Lys63-Linked Ubiquitin Dimer
gi|209735846
Mass: 14793
Score: 263
Queries matched: 19
Ubiquitin [Salmo salar]
Mass: 14735
Score: 263
Queries matched: 19
gi|209879912
60S ribosomal protein L40 [Cryptosporidium muris RN66]
gi|213512766
Mass: 14706
Score: 263
Queries matched: 19
ubiquitin A-52 residue ribosomal protein fusion product 1like 2 [Salmo salar]
gi|219499522
Mass: 13638
Score: 263
Queries matched: 19
hypothetical protein BRAFLDRAFT_189941 [Branchiostoma floridae]
Mass: 8674
Score: 263
Queries matched: 19
gi|223646272
gi|223646368
Mass: 14732
Score: 263
Queries matched: 19
gi|225703194
Mass: 14653
Score: 263
Queries matched: 19
Ubiquitin [Oncorhynchus mykiss]
gi|225705126
Mass: 17972
Score: 263
Queries matched: 19
gi|225710628
Mass: 14675
Score: 263
Queries matched: 19
Ubiquitin [Caligus rogercresseyi]
gi|225714096
Mass: 14691
Score: 263
Queries matched: 19
Ubiquitin [Lepeophtheirus salmonis]
gi|225715160
Mass: 10466
Score: 263
Queries matched: 19
Ubiquitin [Esox lucius]
gi|226371948
Mass: 17964
Score: 263
Queries matched: 19
Ubiquitin [Rana catesbeiana]
gi|229368168
Mass: 17858
Score: 263
Queries matched: 19
Ubiquitin [Anoplopoma fimbria]
Mass: 8558
Score: 261
Queries matched: 19
gi|158763
ubiquitin
Score: 261
Queries matched: 19
gi|158767
gi|119624911
Mass: 16792
Score: 261
Queries matched: 19
ubiquitin B, isoform CRA_e [Homo sapiens]
Mass: 11646
Score: 261
Queries matched: 19
gi|225704224
gi|149063235
Mass: 18245
Score: 260
Queries matched: 19
rCG21224 [Rattus norvegicus]
gi|54300702
Mass: 17142
Score: 258
Queries matched: 19
ubiquitin C splice variant [Homo sapiens]
gi|62079638
Mass: 17851
Score: 258
Queries matched: 19
ubiquitin [Oreochromis mossambicus]
gi|1321735
Mass: 15669
Score: 257
Queries matched: 19
ubiquitin [Carabus alpestris]
gi|26353460
Mass: 17214
Score: 257
Queries matched: 19
unnamed protein product [Mus musculus]
Mass: 17621
Score: 257
Queries matched: 19
gi|54610299
polyubiquitin-like protein [Schistosoma japonicum]
gi|82622379
Mass: 17936
Score: 257
Queries matched: 19
unknown [Theileria lestoquardi]
gi|90075190
Mass: 17892
Score: 257
Queries matched: 19
unnamed protein product [Macaca fascicularis]
gi|7799051
Mass: 17236
Score: 255
Queries matched: 19
ubiquitin [Cyanidium caldarium]
Mass: 22708
Score: 255
Queries matched: 19
gi|14250448
Ubc protein [Mus musculus]
gi|28189917
Mass: 19318
Score: 255
Queries matched: 19
similar to polyubiquitin [Bos taurus]
gi|56199552
Mass: 18776
Score: 255
Queries matched: 19
ubiquitin [Culicoides sonorensis]
Mass: 17479
Score: 255
Queries matched: 19
gi|71033201
ubiquitin [Theileria parva strain Muguga]
gi|73955936
Mass: 17204
Score: 255
Queries matched: 19
PREDICTED: similar to ubiquitin B precursor isoform 3 [Canis familiaris]
gi|84998672
Mass: 17935
Score: 255
Queries matched: 19
ubiquitin [Theileria annulata]
gi|114152964
Mass: 26493
Score: 255
Queries matched: 19
ubiquitin/40S ribosomal protein S27a fusion protein [Argas monolakensis]
gi|149052896
Mass: 17264
Score: 255
Queries matched: 19
rCG34296, isoform CRA_a [Rattus norvegicus]
12/9/2009
Page 7 of 18
gi|197129059
Mass: 21234
Score: 255
Queries matched: 19
putative ubiquitin C variant 4 [Taeniopygia guttata]
gi|197129060
Mass: 17215
Score: 255
Queries matched: 19
gi|209875527
Mass: 17405
Score: 255
Queries matched: 19
ubiquitin family protein [Cryptosporidium muris RN66]
gi|228311825
Mass: 17081
Score: 255
Queries matched: 19
Chain A, Crystal Structure Of Linear Di-Ubiquitin
Mass: 17214
Score: 255
Queries matched: 19
gi|256079432
ubiquitin (ribosomal protein L40) [Schistosoma mansoni]
Mass: 25657
Score: 255
Queries matched: 19
gi|225711930
gi|90076064
Mass: 23571
Score: 255
Queries matched: 19
gi|195587566
Mass: 21780
Score: 255
Queries matched: 19
gi|140083661
Mass: 23510
Score: 254
Queries matched: 19
ubiquitin B [Sus scrofa]
gi|197129049
Mass: 23410
Score: 254
Queries matched: 19
gi|197129052
Mass: 27521
Score: 254
Queries matched: 19
gi|195164961
Mass: 26219
Score: 252
Queries matched: 19
gi|197129065
Mass: 25758
Score: 252
Queries matched: 19
Mass: 25805
Score: 252
Queries matched: 19
gi|225709684
gi|225715800
Mass: 25743
Score: 252
Queries matched: 19
gi|49586
Mass: 25104
Score: 252
Queries matched: 19
unnamed protein product [Cricetulus sp.]
gi|93359417
Mass: 29809
Score: 252
Queries matched: 19
polyubiquitin [Anser anser]
gi|195587564
Mass: 34024
Score: 252
Queries matched: 19
Mass: 25955
Score: 251
Queries matched: 19
gi|158753
ubiquitin
gi|11024714
Mass: 25746
Score: 251
Queries matched: 19
ubiquitin B precursor [Homo sapiens]
Mass: 25806
Score: 251
Queries matched: 19
gi|12833702
gi|37595366
Mass: 26486
Score: 251
Queries matched: 19
ubiquitin C [Danio rerio]
gi|55977015
Mass: 25742
Score: 251
Queries matched: 19
Mass: 25859
Score: 251
Queries matched: 19
gi|61369554
ubiquitin B [synthetic construct]
gi|67616274
Mass: 25774
Score: 251
Queries matched: 19
ubiquitin B [Cryptosporidium hominis TU502]
gi|74214198
Mass: 31250
Score: 251
Queries matched: 19
gi|109497142
Mass: 31226
Score: 251
Queries matched: 19
PREDICTED: similar to ubiquitin C isoform 1 [Rattus norvegicus]
gi|110671522
Mass: 25790
Score: 251
Queries matched: 19
putative ubiquitin B [Diaphorina citri]
gi|149723670
Mass: 34153
Score: 251
Queries matched: 19
PREDICTED: hypothetical protein [Equus caballus]
Mass: 26225
Score: 251
Queries matched: 19
gi|156087036
polyubiquitin [Babesia bovis T2Bo]
gi|156358391
Mass: 25791
Score: 251
Queries matched: 19
gi|195469924
Mass: 25827
Score: 251
Queries matched: 19
gi|197129056
Mass: 27945
Score: 251
Queries matched: 19
Mass: 25757
Score: 251
Queries matched: 19
gi|213510698
polyubiquitin [Salmo salar]
gi|220808512
Mass: 26603
Score: 251
Queries matched: 19
ubiquitin B [Mus musculus]
gi|256072199
Mass: 25643
Score: 251
Queries matched: 19
Mass: 30391
Score: 251
Queries matched: 19
gi|89891990
ubiquitin C II [Anser anser]
gi|221048065
Mass: 28658
Score: 251
Queries matched: 19
ubiquitin C variant [Epinephelus coioides]
gi|223646966
Mass: 34244
Score: 251
Queries matched: 19
gi|12851404
Mass: 34235
Score: 251
Queries matched: 19
gi|444791
Mass: 34393
Score: 250
Queries matched: 19
ubiquitin
12/9/2009
Page 8 of 18
gi|42490818
Mass: 34342
Score: 250
Queries matched: 19
Ubiquitin B [Mus musculus]
gi|27806505
Mass: 34347
Score: 250
Queries matched: 19
polyubiquitin [Bos taurus]
gi|197129051
Mass: 34289
Score: 250
Queries matched: 19
gi|197129053
Mass: 34370
Score: 250
Queries matched: 19
Mass: 34381
Score: 249
Queries matched: 19
gi|12849189
Mass: 34356
Score: 249
Queries matched: 19
gi|18044723
gi|104303708
Mass: 34321
Score: 249
Queries matched: 19
ubiquitin [Rana nigromaculata]
gi|194043617
Mass: 34332
Score: 249
Queries matched: 19
PREDICTED: ubiquitin C isoform 1 [Sus scrofa]
gi|1197093
Mass: 34962
Score: 248
Queries matched: 19
ubiquitin [Cavia porcellus]
gi|6755919
Mass: 34348
Score: 248
Queries matched: 19
gi|15928840
Mass: 34284
Score: 248
Queries matched: 19
UBC protein [Homo sapiens]
gi|19483857
Mass: 39791
Score: 248
Queries matched: 19
gi|21428336
Mass: 34369
Score: 248
Queries matched: 19
GH17513p [Drosophila melanogaster]
Mass: 34305
Score: 248
Queries matched: 19
gi|57163957
polyubiquitin [Ovis aries]
gi|89994036
Mass: 34287
Score: 248
Queries matched: 19
Polyubiquitin [Bos taurus]
gi|126352454
Mass: 34321
Score: 248
Queries matched: 19
ubiquitin C [Equus caballus]
gi|185135461
Mass: 34298
Score: 248
Queries matched: 19
polyubiquitin [Oncorhynchus mykiss]
gi|195170771
Mass: 34458
Score: 248
Queries matched: 19
Mass: 35980
Score: 248
Queries matched: 19
gi|195340400
gi|195403389
Mass: 43087
Score: 248
Queries matched: 19
GJ16068 [Drosophila virilis]
Mass: 35822
Score: 248
Queries matched: 19
gi|195491528
gi|221222312
Mass: 34312
Score: 248
Queries matched: 19
gi|223646702
Mass: 34346
Score: 248
Queries matched: 19
Mass: 39365
Score: 248
Queries matched: 19
gi|256079430
gi|30060222
Mass: 38183
Score: 248
Queries matched: 19
polyubiquitin [Schistosoma japonicum]
gi|197129055
Mass: 45883
Score: 247
Queries matched: 19
gi|16552475
Mass: 43622
Score: 247
Queries matched: 19
gi|56754831
Mass: 42822
Score: 247
Queries matched: 19
unknown [Schistosoma japonicum]
gi|197129067
Mass: 42829
Score: 247
Queries matched: 19
Mass: 42889
Score: 246
Queries matched: 19
gi|2118958
polyubiquitin 5 - Chinese hamster
gi|73695428
Mass: 48309
Score: 246
Queries matched: 19
Ubc protein [Rattus norvegicus]
gi|73995130
Mass: 42825
Score: 246
Queries matched: 19
PREDICTED: similar to CG11624-PA, isoform A [Canis familiaris]
gi|148237866
Mass: 42726
Score: 246
Queries matched: 19
similar to ubiquitin C [Xenopus laevis]
Mass: 42829
Score: 246
Queries matched: 19
gi|149535948
PREDICTED: similar to ubiquitin [Ornithorhynchus anatinus]
gi|195164963
Mass: 42910
Score: 246
Queries matched: 19
gi|209738556
Mass: 42823
Score: 246
Queries matched: 19
Mass: 51381
Score: 244
Queries matched: 19
gi|56753927
SJCHGC00176 protein [Schistosoma japonicum]
gi|169146689
Mass: 51610
Score: 244
Queries matched: 19
gi|189441656
Mass: 51470
Score: 244
Queries matched: 19
gi|195399337
Mass: 51431
Score: 244
Queries matched: 19
gi|196016942
Mass: 51478
Score: 244
Queries matched: 19
hypothetical protein TRIADDRAFT_64418 [Trichoplax adhaerens]
12/9/2009
Page 9 of 18
gi|223646766
Mass: 51365
Score: 244
Queries matched: 19
gi|118150620
Mass: 59884
Score: 243
Queries matched: 19
hypothetical protein LOC777766 [Danio rerio]
gi|24640086
Mass: 59993
Score: 243
Queries matched: 19
Ubiquitin-5E [Drosophila melanogaster]
gi|149254469
Mass: 65416
Score: 243
Queries matched: 19
PREDICTED: similar to Ubc protein isoform 1 [Mus musculus]
Mass: 59923
Score: 243
Queries matched: 19
gi|156358687
Mass: 60127
Score: 243
Queries matched: 19
gi|195011548
GH15714 [Drosophila grimshawi]
gi|213982779
Mass: 59908
Score: 243
Queries matched: 19
hypothetical protein LOC100216108 [Xenopus (Silurana) tropicalis]
gi|112983984
Mass: 102361
Score: 242
Queries matched: 19
polyubiquitin [Bombyx mori]
gi|148687613
Mass: 69069
Score: 242
Queries matched: 19
ubiquitin C, isoform CRA_a [Mus musculus]
gi|1304128
Mass: 68681
Score: 242
Queries matched: 19
polyubiquitin [Homo sapiens]
gi|158256510
Mass: 68406
Score: 242
Queries matched: 19
gi|195042539
Mass: 78569
Score: 242
Queries matched: 19
gi|219418415
Mass: 67453
Score: 242
Queries matched: 19
Mass: 73905
Score: 241
Queries matched: 19
gi|940395
polyubiquitin [Cricetulus sp.]
gi|18256864
Mass: 73958
Score: 241
Queries matched: 19
gi|55742507
Mass: 68450
Score: 241
Queries matched: 19
ubiquitin C [Xenopus (Silurana) tropicalis]
gi|145046250
Mass: 68553
Score: 241
Queries matched: 19
gi|148235355
Mass: 68464
Score: 241
Queries matched: 19
ubiquitin C [Xenopus laevis]
Mass: 68479
Score: 241
Queries matched: 19
gi|157138589
ubiquitin [Aedes aegypti]
gi|194762588
Mass: 68535
Score: 241
Queries matched: 19
GF20300 [Drosophila ananassae]
Mass: 68465
Score: 241
Queries matched: 19
gi|195132655
gi|195137906
Mass: 74931
Score: 241
Queries matched: 19
gi|195448336
Mass: 68569
Score: 241
Queries matched: 19
GK10077 [Drosophila willistoni]
Mass: 78339
Score: 240
Queries matched: 19
gi|510473
polyubiquitin [Artemia franciscana]
gi|9837284
Mass: 82489
Score: 240
Queries matched: 19
polyubiquitin C [Mus musculus]
gi|67191208
Mass: 76982
Score: 240
Queries matched: 19
ubiquitin C [Homo sapiens]
gi|76638716
Mass: 77523
Score: 240
Queries matched: 19
PREDICTED: ubiquitin C isoform 12 [Bos taurus]
gi|340068
Mass: 76992
Score: 240
Queries matched: 19
ubiquitin
gi|46359622
Mass: 77007
Score: 240
Queries matched: 19
polyubiquitin [Crassostrea gigas]
Mass: 76994
Score: 240
Queries matched: 19
gi|86515356
polyubiquitin [Tribolium castaneum]
gi|157671923
Mass: 82499
Score: 240
Queries matched: 19
ubiquitin C [Mus musculus]
gi|8394502
Mass: 91031
Score: 239
Queries matched: 19
ubiquitin C [Rattus norvegicus]
gi|24657014
Mass: 85746
Score: 239
Queries matched: 19
Ubiquitin-63E, isoform A [Drosophila melanogaster]
Mass: 85562
Score: 239
Queries matched: 19
gi|157138587
gi|208022675
Mass: 85533
Score: 239
Queries matched: 19
ubiquitin C [Pan troglodytes]
gi|9837286
Mass: 99515
Score: 238
Queries matched: 19
Mass: 94089
Score: 238
Queries matched: 19
gi|194749344
gi|2627131
Mass: 99531
Score: 238
Queries matched: 19
polyubiquitin [Cricetulus griseus]
gi|195337251
Mass: 102832
Score: 237
Queries matched: 19
gi|2627133
Mass: 116600
Score: 236
Queries matched: 19
gi|194866181
Mass: 111389
Score: 236
Queries matched: 19
GG14240 [Drosophila erecta]
12/9/2009
Page 10 of 18
gi|195125345
Mass: 111410
Score: 236
Queries matched: 19
gi|21429768
Mass: 119913
Score: 236
Queries matched: 19
AT20865p [Drosophila melanogaster]
gi|170034793
Mass: 119729
Score: 236
Queries matched: 19
conserved hypothetical protein [Culex quinquefasciatus]
7.
gi|157165473
Mass: 7421
Score: 237
Queries matched: 7
emPAI: 0.48
sodium-dependent tyrosine transporter [Campylobacter concisus 13826]
Query
44
78
115
133
188
189
235
8.
Observed
441.2397
501.7640
541.2670
563.7799
415.2382
622.3538
698.3726
Mr(expt)
880.4648
1001.5134
1080.5194
1125.5452
1242.6928
1242.6930
1394.7306
Mr(calc)
880.4654
1001.5142
1080.5200
1125.5455
1242.6932
1242.6932
1394.7306
-0.64
1
31
0.76
1
-0.73
0
43
0.079
1
-0.47
1
29
0.97
1
-0.18
0
33
0.91
1
-0.34
1
(17)
22
5
-0.12
1
44
0.043
1
0.02
1
57 0.0017
1
gi|6729922
Mass: 14639
Score: 210
Queries matched: 7
Peptide
R.TYLNKDK.I
K.IDSVQDGIR.I
K.SQRFESEAK.A
R.FYEGANQVAK.S
R.IKIDSVQDGIR.I
R.IKIDSVQDGIR.I
R.IRFYEGANQVAK.S
emPAI: 0.52
Query
4
21
49
246
247
248
279
Observed
362.2289
419.7188
454.2532
734.3329
734.3331
734.3331
798.3806
Mr(expt)
722.4432
837.4230
906.4918
1466.6512
1466.6516
1466.6516
1594.7466
Mr(calc)
722.4439
837.4232
906.4923
1466.6525
1466.6525
1466.6525
1594.7475
ppm Miss Score
Expect Rank
-0.91
0
27
0.67
1
-0.20
0
23
6.2
1
-0.53
0
25
4.8
1
-0.87
0
(74) 2.4e-005
1
-0.60
0
98 1.2e-007
1
-0.60
0
(49)
0.0093
1
-0.52
1
36
0.27
1
Peptide
K.LVHVQK.W
K.NTEISFK.L
K.SLGVGFATR.Q
K.LGVEFDETTADDR.K
K.LGVEFDETTADDR.K
K.LGVEFDETTADDR.K
K.LGVEFDETTADDRK.V
Mass: 14770
Score: 210
Queries matched: 7
gi|27805809
fatty acid binding protein 3 [Bos taurus]
gi|39939390
Mass: 14753
Score: 210
Queries matched: 7
fatty acid-binding protein [Capra hircus]
gi|224760716
Mass: 14784
Score: 210
Queries matched: 7
heart fatty acid-binding protein [Bos taurus]
9.
gi|229552
albumin
Mass: 66088
Score: 202
Queries matched: 7
emPAI: 0.10
Query
65
79
85
144
157
284
285
Observed
487.7326
501.7948
507.8130
571.8605
582.3188
547.3172
820.4728
Mr(expt)
973.4506
1001.5750
1013.6114
1141.7064
1162.6230
1638.9298
1638.9310
Mr(calc)
973.4505
1001.5757
1013.6121
1141.7070
1162.6234
1638.9305
1638.9305
0.15
0
26
1.2
1
-0.66
0
48
0.013
1
-0.62
0
23
2.4
1
-0.51
1
21
1.4
1
-0.27
0
48
0.015
1
-0.43
1
(19)
4.6
1
0.35
1
35
0.085
1
Peptide
K.DLGEEHFK.G
K.LVVSTQTALA.K.QTALVELLK.H
K.KQTALVELLK.H
K.LVNELTEFAK.T
R.KVPQVSTPTLVEVSR.S
R.KVPQVSTPTLVEVSR.S
Mass: 69248
Score: 202
Queries matched: 7
gi|1351907
RecName: Full=Serum albumin; AltName: Full=BSA; AltName: Allergen=Bos d 6; Flags: Precursor
gi|30794280
Mass: 69278
Score: 202
Queries matched: 7
albumin [Bos taurus]
gi|74267962
Mass: 69190
Score: 202
Queries matched: 7
ALB protein [Bos taurus]
gi|154425704
Mass: 69248
Score: 202
Queries matched: 7
10.
gi|15673843
Mass: 43185
Score: 195
Queries matched: 6
elongation factor Tu [Lactococcus lactis subsp. lactis Il1403]
emPAI: 0.25
Query
131
143
145
280
286
287
11.
Observed
561.8029
569.8004
572.8322
800.4386
548.9611
822.9386
gi|181402
Mr(expt)
1121.5912
1137.5862
1143.6498
1598.8626
1643.8615
1643.8626
Mass: 65825
Mr(calc)
1121.5903
1137.5852
1143.6499
1598.8628
1643.8618
1643.8618
Score: 194
0.83
0
45
0.033
1
0.89
0
(30)
1.3
1
-0.07
1
46
0.02
1
-0.08
0
54 0.0029
1
-0.18
1
(24)
5
1
0.53
1
49
0.012
1
Queries matched: 4
Peptide
K.AVVTGIEMFR.K
K.AVVTGIEMFR.K + Oxidation (M)
R.TVGSGIVAEIKA.K.TLTEGLAGDNVGALLR.G
K.VGDEVEIVGIKEETK.K
K.VGDEVEIVGIKEETK.K
emPAI: 0.10
12/9/2009
Page 11 of 18
Query
64
124
196
249
Observed
487.2691
554.2750
627.8072
738.3959
Mr(expt)
972.5236
1106.5354
1253.5998
1474.7772
Mr(calc)
972.5240
1106.5356
1253.6001
1474.7780
-0.33
0
43
0.099
1
-0.14
0
48
0.027
1
-0.16
0
51 0.0073
1
-0.50
0
52 0.0078
1
Peptide
K.IEISELNR.V
K.AQYEEIAQR.S
R.GFSSGSAVVSGGSR.R
R.FLEQQNQVLQTK.W
Mass: 65393
Score: 194
Queries matched: 4
gi|47132620
gi|109096839
Mass: 65462
Score: 194
Queries matched: 4
PREDICTED: similar to keratin 2a [Macaca mulatta]
gi|148727309
Mass: 65944
Score: 194
Queries matched: 4
12.
gi|136429
Mass: 24394
Score: 194
Queries matched: 10
emPAI: 0.67
Query
22
23
24
96
97
104
262
327
328
329
Observed
421.7581
421.7581
421.7581
523.2854
523.2854
532.2579
763.8802
1106.0550
737.7061
737.7062
Mr(expt)
841.5016
841.5016
841.5016
1044.5562
1044.5562
1062.5012
1525.7458
2210.0954
2210.0965
2210.0968
Mr(calc)
841.5022
841.5022
841.5022
1044.5564
1044.5564
1062.5016
1525.7446
2210.0967
2210.0967
2210.0967
ppm Miss Score
Expect Rank
-0.60
0
55
0.0045
1
-0.60
0
(50)
0.015
1
-0.60
0
(47)
0.029
1
-0.10
0
49
0.023
1
-0.10
0
(10) 1.8e+002
1
-0.29
0
16
29
1
0.80
0
19
12
1
-0.58
0
(53)
0.0054
1
-0.11
0
(53)
0.0056
1
0.02
0
54
0.0052
1
Peptide
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
K.LSSPATLNSR.V
K.LSSPATLNSR.V
K.APVLSDSSCK.S + Carbamidomethyl (C)
K.SSGSSYPSLLQCLK.A + Carbamidomethyl (C)
R.LGEHNIDVLEGNEQFINAAK.I
gi|2914482
Mass: 23460
Score: 194
Queries matched: 10
Chain A, Complex Of The Second Kunitz Domain Of Tissue Factor Pathway Inhibitor With Porcine Trypsin
gi|3318722
Mass: 23457
Score: 194
Queries matched: 10
Chain E, Leech-Derived Tryptase InhibitorTRYPSIN COMPLEX
Mass: 23458
Score: 194
Queries matched: 10
gi|110590762
Chain A, Trypsin In Complex With Borate
gi|242253868
Mass: 25865
Score: 194
Queries matched: 10
trypsinogen [Sus scrofa]
13.
gi|123506
Mass: 8353
Score: 191
Queries matched: 7
emPAI: 4.81
Query
68
69
149
160
193
199
210
Observed
492.3033
492.3033
576.7584
583.7664
627.2827
634.2904
644.3454
Mr(expt)
982.5920
982.5920
1151.5022
1165.5182
1252.5508
1266.5662
1286.6762
Mr(calc)
982.5923
982.5923
1151.5029
1165.5186
1252.5506
1266.5663
1286.6765
-0.29
0
49 0.0043
1
-0.29
0
(49) 0.0049
1
-0.61
0
52 0.0038
1
-0.29
0
(39) 0.088
1
0.17
1
(15)
20
1
-0.02
1
37
0.15
1
-0.18
0
52 0.0076
1
Peptide
R.ALGILNLNR.N
R.ALGILNLNR.N
R.SYPSNATCPR.T + Carbamidomethyl (C)
R.SYPSNATCPR.T + Acrylamide (C)
R.SYPSNATCPRT.- + Carbamidomethyl (C)
R.SYPSNATCPRT.- + Acrylamide (C)
R.NLQLILNSCGR.S + Carbamidomethyl (C)
Mass: 12474
Score: 191
Queries matched: 7
gi|5019730
hydrophobic seed protein precursor [Glycine max]
gi|76782253
Mass: 12343
Score: 191
Queries matched: 7
14.
gi|2266432
Mass: 59208
Score: 176
Queries matched: 8
emPAI: 0.24
Query
219
220
276
277
293
294
295
296
Observed
442.5672
663.3475
529.9261
794.3863
560.9684
840.9496
560.9689
840.9500
Mr(expt)
1324.6798
1324.6804
1586.7565
1586.7580
1679.8834
1679.8846
1679.8849
1679.8854
Mr(calc)
1324.6809
1324.6809
1586.7576
1586.7576
1679.8842
1679.8842
1679.8842
1679.8842
ppm Miss Score
Expect Rank
-0.86
0
(19)
23
1
-0.35
0
61
0.0013
1
-0.72
0
36
0.29
1
0.27
0
(27)
1.5
1
-0.52
0
(34)
0.39
1
0.24
0
79 8.6e-006
1
0.37
0
(41)
0.058
1
0.71
0
(59) 0.00097
1
Peptide
K.VAALGSDPHISMK.V
K.VAALGSDPHISMK.V
R.QDENYEEAIVHLK.V
R.QDENYEEAIVHLK.V
R.VSLVEDHIAEGSVAVR.I
12/9/2009
Page 12 of 18
gi|3183510
Mass: 59239
Score: 176
Queries matched: 8
RecName: Full=Butyrophilin subfamily 1 member A1; Short=BT; Flags: Precursor
gi|12647311
Mass: 32265
Score: 176
Queries matched: 8
gi|27806233
Mass: 59225
Score: 176
Queries matched: 8
butyrophilin, subfamily 1, member A1 [Bos taurus]
15.
gi|2851513
Mass: 45695
Score: 155
Queries matched: 3
emPAI: 0.15
RecName: Full=Lactadherin; AltName: Full=Milk fat globule-EGF factor 8; Short=MFG-E8; AltName: Full=MFGM; AltName: Full=Sperm surface protein SP47; S
Query
35
86
113
16.
Observed
434.7115
508.2982
537.7983
Mr(expt)
867.4084
1014.5818
1073.5820
Mr(calc)
867.4086
1014.5822
1073.5829
-0.23
0
35
0.18
1
-0.35
0
66 0.0003
1
-0.84
0
54 0.0038
1
Peptide
K.VAYSTDGR.K
R.VTGIITQGAR.D
R.VTGVVTQGASR.A
gi|3914346
Mass: 82383
Score: 149
Queries matched: 6
RecName: Full=Polymeric immunoglobulin receptor; Short=Poly-Ig receptor; Short=PIGR; Contains: RecName: Full=Secretory component; Flags: Precursor
Query
26
32
62
66
179
Observed
425.2158
432.2236
478.7740
488.2790
604.8079
Mr(expt)
848.4170
862.4326
955.5334
974.5434
1207.6012
Mr(calc)
848.4174
862.4331
955.5338
974.5437
1207.6020
205
640.8825
1279.7504
1279.7500
-0.46
0
(34)
0.68
1
-0.50
0
42
0.13
1
-0.41
0
27
1.4
1
-0.23
0
13 1e+002
4
-0.59
0
46
0.03
1
0.36
1
20
2.1
Peptide
K.CGLGISSR.G + Carbamidomethyl (C)
K.CGLGISSR.G + Acrylamide (C)
K.VVQGEPSLK.V
R.WISTVELK.V
R.TVTINCPFTR.A + Carbamidomethyl (C)
1
K.VVQGEPSLKVPK.N
Mass: 82399
Score: 149
Queries matched: 6
gi|32189338
polymeric immunoglobulin receptor [Bos taurus]
gi|151555693
Mass: 82455
Score: 149
Queries matched: 6
PIGR protein [Bos taurus]
17.
gi|83764016
Mass: 187175
Score: 141
Queries matched: 5
prepro complement component C3 [Bos taurus]
emPAI: 0.02
Query
12
91
142
151
225
Observed
400.7473
514.2981
569.2856
577.2833
681.3360
Mr(expt)
799.4800
1026.5816
1136.5566
1152.5520
1360.6574
Mr(calc)
799.4804
1026.5822
1136.5570
1152.5519
1360.6583
-0.39
0
36
0.34
3
-0.52
0
28
1.4
1
-0.28
0
(30)
1
1
0.15
0
33
0.54
1
-0.60
1
44
0.04
1
Peptide
R.VGLVAVDK.G
R.ISLTHSLTR.V
K.GSMILDICTK.Y + Carbamidomethyl (C)
K.GSMILDICTK.Y + Carbamidomethyl (C); Oxidation (M)
K.SGSDEVQVKQER.K
gi|99028969
Mass: 187112
Score: 141
Queries matched: 5
complement component 3 [Bos taurus]
gi|124056491
Mass: 187135
Score: 141
Queries matched: 5
RecName: Full=Complement C3; Contains: RecName: Full=Complement C3 beta chain; Contains: RecName: Full=Complement C3 alpha chain; Contains: RecName:
18.
gi|62126072
Mass: 46687
endopin 2C [Bos taurus]
Score: 127
Queries matched: 5
emPAI: 0.07
Query
74
114
138
140
181
19.
Observed
497.8057
540.2958
568.3214
568.3215
608.3034
Mr(expt)
993.5968
1078.5770
1134.6282
1134.6284
1214.5922
gi|6680604
Mass: 51574
keratin 16 [Mus musculus]
Mr(calc)
993.5971
1078.5771
1134.6285
1134.6285
1214.5931
-0.26
0
30
0.24
1
-0.04
0
24
4.2
1
-0.19
0
(38)
0.13
1
-0.01
0
49 0.0099
1
-0.73
0
23
3.8
1
Score: 109
Queries matched: 3
Peptide
K.LAVSQVIHK.A
R.HIDELSLPR.F
K.ETQSIIFLGK.V
K.ETQSIIFLGK.V
R.FSISSDYQLR.D
emPAI: 0.06
Query
13
119
121
Observed
404.2033
542.2769
545.7689
Mr(expt)
806.3920
1082.5392
1089.5232
Mr(calc)
806.3923
1082.5396
1089.5237
ppm Miss Score
Expect Rank
-0.26
0
41
0.086
1
-0.35
0
9 1.6e+002
1
-0.40
0
59
0.0013
1
Peptide
R.LAADDFR.T
K.YENELFLR.Q
K.VTMQNLNDR.L
gi|11559579
Mass: 52021
Score: 109
Queries matched: 3
keratin intermediate filament 16a [Mus musculus]
gi|11559580
Mass: 51933
Score: 109
Queries matched: 3
keratin intermediate filament 16b [Mus musculus]
gi|73768791
Mass: 51661
Score: 109
Queries matched: 3
12/9/2009
Page 13 of 18
Keratin 16 [Mus musculus]
20.
gi|15672820
Mass: 44683
Score: 94
Queries matched: 3
30S ribosomal protein S1 [Lactococcus lactis subsp. lactis Il1403]
Query
25
152
214
21.
Observed
421.7582
578.3218
650.8642
Mr(expt)
841.5018
1154.6290
1299.7138
Mr(calc)
841.5022
1154.6295
1299.7147
-0.38
0
37
0.34
1
-0.41
0
25
2.7
1
-0.64
1
33
0.58
1
Peptide
K.VGQVVNVK.V
K.VLDVKPAEER.I
K.APVGSTVEGTVKR.L
gi|77736341
Mass: 52315
Score: 84
Queries matched: 2
emPAI: 0.06
serpin peptidase inhibitor, clade C (antithrombin), member 1 [Bos taurus]
Query
206
227
Observed
641.3093
690.3712
Mr(expt)
1280.6040
1378.7278
Mr(calc)
1280.6037
1378.7279
0.26
0
18
44
1
-0.01
0
67 0.00024
1
gi|109940161
Mass: 52314
Score: 84
Queries matched:
RecName: Full=Antithrombin-III; Short=ATIII; Flags: Precursor
Mass: 52337
Score: 84
Queries matched:
gi|149708147
PREDICTED: similar to antithrombin protein [Equus caballus]
gi|157838185
Mass: 48618
Score: 84
Queries matched:
Chain A, Crystal Structure Of Cleaved Bovine Antithrombin Iii
22.
gi|223165
Mass: 18255
lactoglobulin beta
Score: 76
Peptide
R.SDLYVSDAFHK.A
R.EVALNTIIFMGR.V + Oxidation (M)
2
2
2
At 3.2 Angstroms Resolution
Queries matched: 2
emPAI: 0.19
Query
57
190
23.
Observed
475.2729
623.2958
Mr(expt)
948.5312
1244.5770
Mr(calc)
948.5314
1244.5772
-0.16
0
21
9.4
1
-0.13
0
55 0.0024
1
gi|15900538
Mass: 24480
Score: 71
Queries matched: 1
50S ribosomal protein L1 [Streptococcus pneumoniae TIGR4]
Peptide
-.IIVTQTMK.G + Oxidation (M)
R.TPEVDDEALEK.F
emPAI: 0.14
Query
192
Observed
625.8344
Mr(expt)
1249.6542
Mr(calc)
1249.6554
-0.91
0
71 9e-005
1
Peptide
K.AYSVEEAVALAK.E
gi|22537662
Mass: 24278
Score: 71
Queries matched: 1
50S ribosomal protein L1 [Streptococcus agalactiae 2603V/R]
gi|24380006
Mass: 24495
Score: 71
Queries matched: 1
50S ribosomal protein L1 [Streptococcus mutans UA159]
Mass: 24445
Score: 71
Queries matched: 1
gi|29377196
50S ribosomal protein L1 [Enterococcus faecalis V583]
gi|116512945
Mass: 23935
Score: 71
Queries matched: 1
50S ribosomal protein L1 [Lactococcus lactis subsp. cremoris SK11]
Mass: 24452
Score: 71
Queries matched: 1
gi|125718427
50S ribosomal protein L1 [Streptococcus sanguinis SK36]
gi|148988998
Mass: 24508
Score: 71
Queries matched: 1
ribosomal protein L1 [Streptococcus pneumoniae SP6-BS73]
gi|157150680
Mass: 24399
Score: 71
Queries matched: 1
50S ribosomal protein L1 [Streptococcus gordonii str. Challis substr. CH1]
gi|171780177
Mass: 24496
Score: 71
Queries matched: 1
hypothetical protein STRINF_01965 [Streptococcus infantarius subsp. infantarius ATCC BAA-102]
gi|225854162
Mass: 24508
Score: 71
Queries matched: 1
ribosomal protein L1 [Streptococcus pneumoniae JJA]
gi|225860629
Mass: 24427
Score: 71
Queries matched: 1
ribosomal protein L1 [Streptococcus pneumoniae Taiwan19F-14]
gi|225868099
Mass: 24444
Score: 71
Queries matched: 1
50S ribosomal protein L1 [Streptococcus equi subsp. zooepidemicus]
24.
gi|2232299
Mass: 47885
Score: 67
Queries matched: 2
emPAI: 0.14
Query
330
331
Observed
740.7206
1110.5780
Mr(expt)
2219.1400
2219.1414
Mr(calc)
2219.1434
2219.1434
-1.55
0
(55) 0.0036
1
-0.88
0
67 0.00021
1
Peptide
R.IVSGISEGQVETVQSSPVTFR.A
gi|3834667
Mass: 48183
Score: 67
Queries matched: 2
immunoglobulin heavy chain constant region [Bos taurus]
12/9/2009
gi|24496448
immunoglobulin
gi|33413902
immunoglobulin
gi|94981786
immunoglobulin
25.
Page 14 of 18
Mass: 65621
Score: 67
Queries matched: 2
M heavy chain secretory form [Bos taurus]
Mass: 49106
Score: 67
Queries matched: 2
mu heavy chain constant region; IgM heavy chain constant region [Bos taurus]
Mass: 21826
Score: 67
Queries matched: 2
heavy chain [Bos taurus]
gi|28592070
Mass: 49551
Score: 66
Queries matched: 2
IgM heavy chain constant region, secretory form [Bos taurus]
emPAI: 0.07
Query
332
333
Observed
1117.5870
745.3939
Mr(expt)
2233.1594
2233.1599
Mr(calc)
2233.1590
2233.1590
0.18
0
66 0.00022
1
0.37
0
(25)
2.8
1
Peptide
R.IVSGISEGQVETVQSSPITFR.A
gi|28592071
Mass: 52023
Score: 66
Queries matched: 2
IgM heavy chain constant region, membrane form [Bos taurus]
gi|34538498
Mass: 49909
Score: 66
Queries matched: 2
gi|94981788
Mass: 21762
Score: 66
Queries matched: 2
immunoglobulin heavy chain [Bos taurus]
26.
gi|6320157
Mass: 19051
Score: 66
Queries matched: 2
emPAI: 0.18
Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention
Query
1
186
Observed
353.7156
617.8559
Mr(expt)
705.4166
1233.6972
Mr(calc)
705.4174
1233.6969
-1.01
0
30
1
1
0.30
0
36
0.16
1
Peptide
K.YVVVAR.A
K.IVEIPGEVPPGK.Y
gi|151941952
Mass: 19021
Score: 66
Queries matched: 2
Niemann Pick type C-like protein [Saccharomyces cerevisiae YJM789]
gi|190405057
Mass: 19007
Score: 66
Queries matched: 2
phosphatidylglycerol/phosphatidylinositol transfer protein precursor [Saccharomyces cerevisiae RM11-1a]
27.
gi|538275
Mass: 22697
Score: 61
Queries matched: 1
emPAI: 0.15
Query
130
Observed
561.3137
Mr(expt)
1120.6128
Mr(calc)
1120.6128
0.02
0
61 0.00069
1
Peptide
K.DTQSIIFLGK.V
gi|1245695
Mass: 28553
Score: 61
Queries matched: 1
alpha 1-antichymotrypsin; ACT [Bos taurus]
Mass: 9788
Score: 61
Queries matched: 1
gi|1245697
gi|31340900
Mass: 46175
Score: 61
Queries matched: 1
serpin peptidase inhibitor, clade A, member 3 [Bos taurus]
gi|84000377
Mass: 46297
Score: 61
Queries matched: 1
endopin 1b [Bos taurus]
gi|121531628
Mass: 46282
Score: 61
Queries matched: 1
SERPINA3-4 [Bos taurus]
gi|126165236
Mass: 46368
Score: 61
Queries matched: 1
hypothetical protein LOC617667 [Bos taurus]
gi|126165264
Mass: 46888
Score: 61
Queries matched: 1
gi|151554250
Mass: 46935
Score: 61
Queries matched: 1
gi|160332365
Mass: 46208
Score: 61
Queries matched: 1
RecName: Full=Serpin A3-1; AltName: Full=Endopin-1A; AltName: Full=Muscle endopin-1A; Short=mEndopin-1A; Flags: Precursor
gi|194038345
Mass: 38368
Score: 61
Queries matched: 1
PREDICTED: similar to alpha-1-antichymotrypsin 2 [Sus scrofa]
gi|194038353
Mass: 47192
Score: 61
Queries matched: 1
Mass: 46208
Score: 61
Queries matched: 1
gi|226373737
SERPIN A3-2 [Bos taurus]
gi|226373739
Mass: 46361
Score: 61
Queries matched: 1
serpin A3-6 [Bos taurus]
28.
gi|149458033
Score: 61
Queries matched: 2
PREDICTED: similar to chymotrypsin C (caldecrin), partial [Ornithorhynchus anatinus]
Query
9
47
Observed
393.7394
451.2659
Mr(expt)
785.4642
900.5172
Mr(calc)
785.4647
900.5181
-0.54
0
28
2.5
1
-0.96
0
33
0.94
1
Peptide
R.NDIALIK.L
K.WNSLLIR.N
12/9/2009
29.
gi|4927746
Mass: 23083
Score: 60
Queries matched: 2
Page 15 of 18
emPAI: 0.15
Query
315
316
Observed
924.4004
924.4023
Mr(expt)
1846.7862
1846.7900
Mr(calc)
1846.7897
1846.7897
-1.88
0
60 0.00037
1
0.18
0
(35)
0.12
1
Peptide
R.ADIDYAWEEADTTYGK.L
gi|15674075
Mass: 24019
Score: 60
Queries matched: 2
gi|15674293
Mass: 24119
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Streptococcus pyogenes M1 GAS]
gi|15900151
Mass: 24031
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Streptococcus pneumoniae TIGR4]
gi|16801836
Mass: 24527
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Listeria innocua Clip11262]
gi|24378382
Mass: 23159
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Streptococcus mutans UA159]
gi|29374857
Mass: 24355
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Enterococcus faecalis V583]
gi|47093991
Mass: 23580
Score: 60
Queries matched: 2
ribosomal protein S3 [Listeria monocytogenes str. 4b H7858]
gi|47097197
Mass: 17247
Score: 60
Queries matched: 2
ribosomal protein S3 [Listeria monocytogenes str. 1/2a F6854]
Mass: 24058
Score: 60
Queries matched: 2
gi|55821899
30S ribosomal protein S3 [Streptococcus thermophilus LMG 18311]
Mass: 24333
Score: 60
Queries matched: 2
gi|69247175
ribosomal protein S3 [Enterococcus faecium DO]
gi|76798223
Mass: 23171
Score: 60
Queries matched: 2
ribosomal protein S3 [Streptococcus agalactiae 18RS21]
gi|116513048
Mass: 24003
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Lactococcus lactis subsp. cremoris SK11]
Mass: 24119
Score: 60
Queries matched: 2
gi|125716991
30S ribosomal protein S3 [Streptococcus sanguinis SK36]
gi|157151263
Mass: 24050
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Streptococcus gordonii str. Challis substr. CH1]
gi|161486819
Mass: 24107
Score: 60
Queries matched: 2
Mass: 24161
Score: 60
Queries matched: 2
gi|171778382
Mass: 24163
Score: 60
Queries matched: 2
gi|195977214
30S ribosomal protein S3 [Streptococcus equi subsp. zooepidemicus MGCS10565]
gi|222152271
Mass: 24134
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Streptococcus uberis 0140J]
gi|228478008
Mass: 24106
Score: 60
Queries matched: 2
ribosomal protein S3 [Streptococcus salivarius SK126]
gi|229557129
Mass: 24458
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Listeria grayi DSM 20601]
gi|251781521
Mass: 24147
Score: 60
Queries matched: 2
30S ribosomal protein S3 [Streptococcus dysgalactiae subsp. equisimilis GGS_124]
gi|256618374
Mass: 24311
Score: 60
Queries matched: 2
ribosomal protein S3 [Enterococcus faecalis ATCC 4200]
30.
gi|15672484
Mass: 9670
Score: 51
Queries matched: 1
emPAI: 0.36
HU like DNA-binding protein [Lactococcus lactis subsp. lactis Il1403]
Query
87
Observed
508.8107
Mr(expt)
1015.6068
Mr(calc)
1015.6066
0.23
0
51 0.0027
1
Peptide
K.IAATVVPAFK.A
Peptide matches not assigned to protein hits: (no details means no match)
Query
7
103
120
122
12
271
98
71
70
6
139
18
163
60
Observed
383.2198
531.3137
543.3323
548.2876
400.7473
783.8732
525.7948
492.7953
492.7951
383.2198
568.3214
416.7478
588.8116
477.7714
Mr(expt)
764.4250
1060.6128
1084.6500
1094.5606
799.4800
1565.7318
1049.5750
983.5760
983.5756
764.4250
1134.6282
831.4810
1175.6086
953.5282
Mr(calc)
764.4255
1060.6128
1084.6492
1094.5608
799.4804
1565.7283
1049.5757
983.5764
983.5764
764.4255
1134.6284
831.4814
1175.6121
953.5294
ppm Miss Score
-0.56
0
48
0.02
0
47
0.78
0
44
-0.13
0
42
-0.39
0
42
2.26
1
40
-0.65
0
39
-0.31
0
37
-0.72
0
37
-0.56
0
37
-0.18
0
36
-0.42
0
36
-2.92
1
35
-1.23
0
34
Expect Rank
0.0062
1
0.009
1
0.026
1
0.055
1
0.099
1
0.097
1
0.12
1
0.13
1
0.14
1
0.083
1
0.2
1
0.27
1
0.39
1
0.2
1
Peptide
MQFVIK
LSVVNISTTK
ILIIGESGVGK
DTQSIIFSGK
VGIVADVK
ADIFELEMDPDKK + Oxidation (M)
DTGTVLFLGK
NAGLILLDR
QILINLDR
MAGVFLK
AVAEYIQTLK
ISSISGLR
EWAMLLNKR + Oxidation (M)
GPQAVNIQK
12/9/2009
Page 16 of 18
309
222
102
272
20
172
41
212
148
30
890.4472
677.3485
530.7943
783.8737
419.7188
596.3179
437.7532
645.8319
574.8066
428.7661
1778.8798
1352.6824
1059.5740
1565.7328
837.4230
1190.6212
873.4918
1289.6492
1147.5986
855.5176
1778.8799
1352.6823
1059.5746
1565.7283
837.4232
1190.6217
873.4920
1289.6537
1147.5985
855.5178
-0.02
0.08
-0.56
2.90
-0.19
-0.34
-0.14
-3.43
0.09
-0.15
1
0
0
1
1
0
0
0
0
0
34
34
34
34
32
31
31
31
30
30
0.51
0.44
0.63
0.39
0.75
0.69
1.9
1
1.5
1.2
1
1
1
1
1
1
1
1
1
1
QLEDGRTVTDYNIQK
TSGELDIYNITK
MSITLPINR + Oxidation (M)
KSEFAEK
TIDNDIMLIK + Oxidation (M)
ISVINGGSK
VEQMIAELETK
ENLQFSAALR
LALSISPR
77
37
146
176
29
162
180
202
201
36
88
334
238
82
501.2847
435.7738
573.3297
597.3420
428.7659
585.3426
605.8375
639.3043
638.8484
435.7737
510.7715
809.7346
713.8464
503.7636
1000.5548
869.5330
1144.6448
1192.6694
855.5172
1168.6706
1209.6604
1276.5940
1275.6822
869.5328
1019.5284
2426.1820
1425.6782
1005.5126
1000.5553
869.5334
1144.6452
1192.6677
855.5178
1168.6703
1209.6605
1276.5904
1275.6823
869.5334
1019.5321
2426.1826
1425.6776
1005.5165
-0.43
-0.46
-0.28
1.50
-0.65
0.28
-0.04
2.87
-0.01
-0.69
-3.59
-0.27
0.46
-3.81
1
0
1
0
0
0
0
0
1
0
1
0
0
0
30
30
29
28
28
28
28
28
28
26
26
26
26
25
1.6
1.3
1.2
0.68
1.9
0.9
0.94
1.6
2.3
3.1
4.8
2.9
2.8
5.7
1
1
1
1
1
1
1
1
1
1
1
1
1
1
IKIDIDER
VVSLSIPR
VLSAADKNTVK
GGGHAGLGLSIVR
LATVVTPR
LIDIIEPTQK
VIAPIDDTPAAK
MMQNGELVPAR + 2 Oxidation (M)
FLEEQQKINK
VVSLSIPR
SIEMKVEGK
VVVGEHNLNQNDGTEQYVGVQK
DFTPELQASYQK
MLADQVSVK + Oxidation (M)
127
154
270
10
218
257
54
42
16
306
265
53
50
203
108
126
58
191
273
76
275
211
215
184
171
168
136
234
559.2480
579.3351
781.8723
395.2389
662.3342
508.5984
467.2754
439.7344
409.7237
887.9523
768.3881
465.7653
454.7214
639.3279
534.2762
556.3395
475.7143
625.3341
785.8724
500.2263
789.3533
644.8372
651.8452
617.3368
595.7941
590.3164
565.7697
465.2490
1116.4814
1156.6556
1561.7300
788.4632
1322.6538
1522.7734
932.5362
877.4542
817.4328
1773.8900
1534.7616
929.5160
907.4282
1276.6412
1066.5378
1110.6644
949.4140
1248.6536
1569.7302
998.4380
1576.6920
1287.6598
1301.6758
1232.6590
1189.5736
1178.6182
1129.5248
1392.7252
1116.4798
1156.6564
1561.7300
788.4644
1322.6541
1522.7740
932.5365
877.4545
817.4334
1773.8872
1534.7562
929.5182
907.4255
1276.6380
1066.5342
1110.6648
949.4109
1248.6577
1569.7344
998.4379
1576.6908
1287.6605
1301.6762
1232.6587
1189.5761
1178.6156
1129.5251
1392.7296
1.52
-0.65
0.00
-1.40
-0.15
-0.38
-0.24
-0.30
-0.69
1.62
3.53
-2.30
2.99
2.56
3.45
-0.35
3.26
-3.21
-2.67
0.15
0.78
-0.52
-0.24
0.25
-2.07
2.22
-0.22
-3.17
0
1
0
1
0
1
1
0
0
1
1
0
0
1
1
0
0
1
1
0
0
0
0
0
0
1
0
1
25
25
25
25
24
24
24
23
22
22
21
21
21
21
21
21
20
20
19
19
19
19
18
18
18
18
17
17
0.89
2.9
3.1
2.3
4.7
5
3.5
5.3
3.4
11
11
14
7.9
13
11
2.2
7.4
15
12
7.1
7.5
18
18
23
24
16
11
19
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
FICESEFDK
LAGIDSIRGQK
YVEGFADGEVVYSK
LVKELTS
TVSASGVTFLMPN
IQEKEGVPPDQQR
ILVDKMAK + Oxidation (M)
APDYATIK
VDVNPFK
MFIYKDINYAINNR
MKNDTVGLGVGSWR + Oxidation (M)
DVEISIVR
MKPNGSMK + Oxidation (M)
MLTNLGMARDR
RYCQAISR + Acrylamide (C)
VLPGLAALTEK
ACMQAPSR + Acrylamide (C); Oxidation (M)
VLFKFYSSMK
NMSFALEEQTKEK + Oxidation (M)
DVDYMVNK + Oxidation (M)
CGAGARPPEMSQMR + Acrylamide (C); Oxidation (M)
VAIVGAALSDCGR + Carbamidomethyl (C)
VAIVGAALSDCGR + Acrylamide (C)
VLFQPGSMQVK
EGDNATCLIVR
FTGTRSQVQR
EQLAEIENGQ
TLSAAGRCGFIAAR
188
204
141
92
66
14
100
38
292
132
48
67
34
118
415.2382
639.3412
568.8060
515.7925
488.2790
405.2242
529.2728
436.2658
832.4364
562.8210
453.7500
490.2694
433.7217
541.7427
1242.6928
1276.6678
1135.5974
1029.5704
974.5434
808.4338
1056.5310
870.5170
1662.8582
1123.6274
905.4854
978.5242
865.4288
1081.4708
1242.6945
1276.6697
1135.5947
1029.5706
974.5437
808.4330
1056.5314
870.5175
1662.8538
1123.6278
905.4827
978.5247
865.4294
1081.4710
-1.42
-1.43
2.42
-0.17
-0.23
0.99
-0.34
-0.49
2.66
-0.28
3.06
-0.43
-0.60
-0.13
1
0
1
0
0
0
0
1
0
1
0
0
0
1
17
17
17
17
17
16
16
16
16
16
16
15
15
15
18
27
21
23
41
25
22
27
24
13
28
27
40
19
1
1
1
1
1
1
1
1
1
1
1
1
1
1
RPVRFQDAVR
MILGDTILETR + Oxidation (M)
KMIENFLEL
LASITDPSVK
LSVTWEIK
LAVESYK
FMLGEGFLK + Oxidation (M)
SVVKDPVK
IPEAYESLILDCLK + Carbamidomethyl (C)
FVPKTTGYIV
MSVCVVLR
HVTLEQPR
GLSPSYSR
GMTSGDDKAGK + Oxidation (M)
155
290
129
170
161
282
40
61
253
581.7905
555.2929
560.7744
595.7939
584.2585
810.9030
437.2187
478.2533
752.8675
1161.5664
1662.8569
1119.5342
1189.5732
1166.5024
1619.7914
872.4228
954.4920
1503.7204
1161.5634
1662.8539
1119.5342
1189.5761
1166.4986
1619.7977
872.4239
954.4957
1503.7252
2.61
1.82
0.02
-2.41
3.29
-3.88
-1.26
-3.79
-3.16
1
0
1
0
1
1
0
0
1
15
15
14
14
14
14
14
14
14
50
44
48
61
23
55
26
46
47
1
1
1
1
1
1
1
1
1
MNGEILCRIN
IFLADGDSLVLPMEK + Oxidation (M)
CRSEEAQIK + Carbamidomethyl (C)
EGDNATCLIVR
GGKDGCSASGGSK + Carbamidomethyl (C)
LEDMNVWVKDLSR + Oxidation (M)
APTATLEGN
LLTAICHN + Acrylamide (C)
INNNVHEMARYK + Oxidation (M)
12/9/2009
Page 17 of 18
134
90
81
89
303
55
217
158
84
156
59
563.7852
514.2800
503.2944
512.2404
574.9761
467.7309
655.3228
582.8110
505.2690
581.8029
475.7285
1125.5558
1026.5454
1004.5742
1022.4662
1721.9065
933.4472
1308.6310
1163.6074
1008.5234
1161.5912
949.4424
1125.5601
1026.5458
1004.5767
1022.4703
1721.9002
933.4477
1308.6310
1163.6074
1008.5249
1161.5924
949.4426
-3.74
-0.35
-2.45
-3.92
3.66
-0.51
0.04
0.05
-1.41
-1.00
-0.19
0
0
0
0
1
0
1
0
0
1
1
14
13
13
13
13
13
13
12
12
12
12
52
29
36
68
39
58
57
85
57
81
45
1
1
1
1
1
1
1
1
1
1
1
LLECLGHGER
AVPAGTEGGIR
LQGIPGAPPR
TSMGDQIGSK
AGAVGAAALWDPPGRWK
MDILSPDK + Oxidation (M)
DAGFRTTELDGK
VLDEIGVEYK
MVVAICAFR
CAAAATTARQK + Acrylamide (C)
KSDIDMEI
266
268
297
99
73
231
250
107
224
45
289
135
83
291
768.3883
773.3852
842.8995
526.7578
496.7979
695.8463
744.8757
533.7653
679.3630
448.7181
832.4355
564.2775
505.2689
555.2930
1534.7620
1544.7558
1683.7844
1051.5010
991.5812
1389.6780
1487.7368
1065.5160
1356.7114
895.4216
1662.8564
1126.5404
1008.5232
1662.8572
1534.7627
1544.7545
1683.7848
1051.5046
991.5815
1389.6736
1487.7402
1065.5124
1356.7112
895.4182
1662.8498
1126.5441
1008.5240
1662.8577
-0.43
0.90
-0.19
-3.42
-0.20
3.22
-2.26
3.39
0.22
3.89
4.00
-3.23
-0.73
-0.30
1
1
1
1
1
1
0
0
1
1
1
0
1
1
12
12
12
11
11
11
11
11
11
11
11
11
10
10
99
85
78
79
30
95
92
1e+002
1.2e+002
99
1.2e+002
81
84
1.2e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
SEGNFLILEDDRK
FMGYLKLGEEGSSK
DVLEYAKCSEICIQ + Acrylamide (C)
AEYDAARTR
KTLPAPTHK
TIIIEDNRSTDN
DVAALAQVMTEANR
SLTSLNSNCK
KQFLIDTFAMK + Oxidation (M)
RTSSGTCK + Carbamidomethyl (C)
ESCKELDSAILLGSK + Acrylamide (C)
KPPEHMSTGK + Oxidation (M)
ETLASYGRL
KLVYEQGSIQEQNK
101
304
111
169
274
288
125
320
322
51
33
5
324
283
75
123
173
207
237
300
17
263
43
200
150
302
251
319
530.7688
881.9338
356.5450
591.7640
788.3864
832.4351
556.3164
970.9708
995.4753
458.2603
432.7490
383.2196
995.4760
813.9698
499.2997
550.2452
597.3242
641.8486
711.8469
857.4075
414.7617
511.2590
440.2035
635.2745
577.2510
861.9603
498.5828
647.6490
1059.5230
1761.8530
1066.6132
1181.5134
1574.7582
1662.8556
1110.6182
1939.9270
1988.9360
914.5060
863.4834
764.4246
1988.9374
1625.9250
996.5848
1098.4758
1192.6338
1281.6826
1421.6792
1712.8004
827.5088
1530.7552
878.3924
1268.5344
1152.4874
1721.9060
1492.7266
1939.9252
1059.5270
1761.8547
1066.6135
1181.5169
1574.7545
1662.8545
1110.6219
1939.9197
1988.9383
914.5073
863.4865
764.4255
1988.9369
1625.9253
996.5855
1098.4764
1192.6353
1281.6830
1421.6787
1712.8014
827.5116
1530.7500
878.3956
1268.5312
1152.4870
1721.9060
1492.7265
1939.9284
-3.75
-0.91
-0.29
-2.91
2.40
0.67
-3.33
3.81
-1.13
-1.38
-3.51
-1.08
0.26
-0.16
-0.68
-0.50
-1.19
-0.25
0.42
-0.58
-3.36
3.35
-3.64
2.59
0.42
0.01
0.02
-1.68
0
1
0
0
1
1
0
0
1
0
0
0
1
1
0
0
1
0
0
1
1
1
0
0
0
0
0
0
10
10
10
10
10
10
10
10
10
9
9
9
9
9
9
9
9
9
9
9
9
9
8
8
8
8
8
7
1.3e+002
1.2e+002
51
53
1e+002
1.3e+002
76
1.2e+002
1e+002
1.7e+002
1.9e+002
51
1.3e+002
37
42
39
1.3e+002
1.1e+002
1.5e+002
1.2e+002
95
1.9e+002
1.3e+002
62
76
1.3e+002
1.9e+002
2.2e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
EIQEMIGPK + Oxidation (M)
WPNAANSNGSVGASRFK
NIGPEGVLLR
VTSSSCCTPR + 2 Acrylamide (C)
EGRVLCEMPALADR + Oxidation (M)
VSGCLIVCIERSTR + Acrylamide (C); Carbamidomethyl (C)
TMPPVQVALR
MLEINNLHVDLEEEDK
SLVPSCHAKWCVTTCR + 2 Acrylamide (C); Carbamidomethyl (C)
DGSVTPVLK
ASILSAFR
IGGFMIK
IAPSDPLPKTVCEGCMNK + Acrylamide (C); Oxidation (M)
GLISRLEISFVHQK
IPDAIDLIK
SLYDGSNMGR
HAFIKHSPEK
HGGTLALGANPFK
NAYSLLSDPADTR
CFNCGKPLEFKDGK + Acrylamide (C); Carbamidomethyl (C)
LVLELKN
NDMLYASFTRGIK + Oxidation (M)
DFVPMDR
TNLCDVNMMR + Carbamidomethyl (C); Oxidation (M)
FCSVPSENDR
AVQLSGGEQLLIDSHR
VDESIPMIAMVTR + 2 Oxidation (M)
MQYDAAVVGAGPAGLMFAR + Oxidation (M)
278
31
267
245
321
318
11
314
323
164
56
308
27
213
794.8645
428.7785
769.3243
729.8594
972.9586
965.4531
399.1927
897.9108
995.4758
589.3429
472.2875
890.4465
425.7607
650.3689
1587.7144
855.5424
1536.6340
1457.7042
1943.9026
1928.8916
796.3708
1793.8070
1988.9370
1176.6712
942.5604
1778.8784
849.5068
1298.7232
1587.7174
855.5430
1536.6337
1457.7006
1943.9081
1928.8898
796.3715
1793.8045
1988.9414
1176.6754
942.5610
1778.8799
849.5073
1298.7194
-1.86
-0.59
0.23
2.48
-2.79
0.96
-0.86
1.43
-2.20
-3.54
-0.62
-0.81
-0.49
2.96
1
0
0
1
1
1
0
0
0
1
1
1
0
0
7
7
7
7
7
7
6
6
6
6
6
6
6
6
1.2e+002
57
33
1.9e+002
1.8e+002
2.2e+002
1.1e+002
2.6e+002
2.4e+002
1.2e+002
2.4e+002
3.3e+002
1.5e+002
2.6e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
CFDQLDVFCRTK + 2 Carbamidomethyl (C)
EGVVIVLK
NCIGQHFAMDEEK + Oxidation (M)
SCTRCYLLEATK + Acrylamide (C)
VNYAKGCSLVSMDESGIR + Oxidation (M)
GSMDPLQKHSDIIDDSR + Oxidation (M)
IGEVHEGG
MSVICMNHCSSLLQR + Carbamidomethyl (C); Oxidation (M)
HANSVLDELWYVMGNNK
FIVVESDKLK
RQISSKPK
QLEDGRTVTDYNIQK
QVVVHLGV
IVDLQVDALASR
325
281
208
264
305
317
209
335
39
1006.4890
810.8333
642.3059
766.3854
883.8956
964.9625
642.3402
810.0602
436.7748
2010.9634
1619.6520
1282.5972
1530.7562
1765.7766
1927.9104
1282.6658
2427.1588
871.5350
2010.9608
1619.6522
1282.6016
1530.7572
1765.7788
1927.9028
1282.6703
2427.1542
871.5379
1.29
-0.09
-3.41
-0.64
-1.24
3.97
-3.51
1.87
-3.22
0
0
0
1
0
0
1
1
0
5
5
5
4
4
4
4
4
3
3e+002
42
2.9e+002
4.3e+002
2.2e+002
4e+002
3.6e+002
4.5e+002
2.5e+002
1
1
1
1
1
1
1
1
1
TTLFTPPVYIEGEQEGCK
MSDPEDWESQPQR + Oxidation (M)
YCDVDPVIFR + Carbamidomethyl (C)
TSGRPRAIEEEMR
TAVSSSESSENSTHCIK
WGTMLLGMLMICSAANK + Carbamidomethyl (C); 2 Oxidation (M)
MDLDVRNPVPK
HLQEFHINGFDHNFCLKGSK + Carbamidomethyl (C)
VGSIASIIL
12/9/2009
28
194
112
298
195
166
46
165
2
3
8
428.2615
627.7686
535.2576
843.3913
627.7746
589.3433
450.2747
589.3429
357.6369
362.2221
384.2351
854.5084
1253.5226
1068.5006
1684.7680
1253.5346
1176.6720
898.5348
1176.6712
713.2592
722.4296
766.4556
15
52
63
72
80
94
128
137
147
178
182
269
406.2482
458.7879
480.8009
494.3005
502.8140
516.3138
559.8738
567.3306
574.2828
603.8997
610.7259
777.8370
810.4818
915.5612
959.5872
986.5864
1003.6134
1030.6130
1117.7330
1132.6466
1146.5510
1205.7848
1219.4372
1553.6594
854.5086
1253.5194
1068.4988
1684.7614
1253.5380
1176.6754
898.5348
1176.6689
-0.20
2.58
1.69
3.94
-2.68
-2.86
0.01
2.02
1
0
0
0
0
0
0
1
3
3
3
2
2
1
1
0
2.2e+002
1.6e+002
4.4e+002
4.7e+002
2.9e+002
4e+002
9.5e+002
4.8e+002
Page 18 of 18
1
1
1
1
1
1
1
1
RVPALSGR
MDDDNVSVTSR + Oxidation (M)
GWPEPEAQR
DMSDDGVLTTLNNYK
GEMQLEMTSGR + Oxidation (M)
GYLGISLEVVK
RPVLSATR
QLKMPYILR + Oxidation (M)
Search Parameters
Type of search
:
Enzyme
:
Variable modifications :
Mass values
:
Protein Mass
:
Peptide Mass Tolerance :
Fragment Mass Tolerance:
Max Missed Cleavages
:
Instrument type
:
Number of queries
:
MS/MS Ion Search
Trypsin
Acrylamide (C),Carbamidomethyl (C),Oxidation (M)
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
335
Mascot: http://www.matrixscience.com/
12/9/2009
Page 1 of 19
User
Email
Search title
MS data file
Database
Timestamp
Protein hits
:
:
:
:
:
:
:
LTQFT Daemon
D:\Data\Mark\18_7-09\N_18_7_09_2.RAW
9 Dec 2009 at 03:56:04 GMT
gi|158604950 thiosulfate sulfurtransferase [Campylobacter concisus 13826]
gi|157165660 co-chaperonin GroES [Campylobacter concisus 13826]
gi|136429
gi|229552
albumin
gi|6729922
gi|2136760
gi|157165021 thioredoxin [Campylobacter concisus 13826]
gi|2266432
gi|2232299
gi|28592070 IgM heavy chain constant region, secretory form [Bos taurus]
gi|15673061 50S ribosomal protein L21 [Lactococcus lactis subsp. lactis Il1403]
gi|122411
RecName: Full=Hemoglobin subunit alpha; AltName: Full=Hemoglobin alpha chain; AltName: Full=Alpha-globin
gi|9725
gi|3914346
gi|7576937
extracellular glyceraldehyde-3-phosphate dehydrogenase [Streptococcus gordonii]
gi|7331218
gi|6320157
gi|123506
gi|15673843 elongation factor Tu [Lactococcus lactis subsp. lactis Il1403]
gi|157165473 sodium-dependent tyrosine transporter [Campylobacter concisus 13826]
gi|4927746
gi|28317
gi|223165
lactoglobulin beta
gi|538275
gi|157164029 protease DO [Campylobacter concisus 13826]
gi|157164081 antibiotic biosynthesis monooxygenase [Campylobacter concisus 13826]
gi|154174129 hypothetical protein CCV52592_0624 [Campylobacter curvus 525.92]
31
0
0.00 %
49
0
0.00 %
Format As
Standard scoring
Show sub-sets 0
Require bold red
Show pop-ups
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1.
Help
Peptide Summary
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MudPIT scoring
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Search Selected
Error tolerant
Archive Report
gi|158604950
Mass: 13498
Score: 312
Queries matched: 8
thiosulfate sulfurtransferase [Campylobacter concisus 13826]
emPAI: 0.57
Query
60
65
88
104
Observed
505.2633
511.2691
558.3005
575.3165
Mr(expt)
1008.5120
1020.5236
1114.5864
1148.6184
Mr(calc)
1008.5128
1020.5240
1114.5870
1148.6190
ppm Miss Score
-0.70
0
30
-0.35
0
35
-0.49
0
30
-0.44
1
45
Expect Rank
0.86
2
0.93
1
0.95
1
0.046
1
Peptide
K.SAFLDELSK.A
K.YDQIVDIR.T
K.TVNISPDEIK.K
K.KYDQIVDIR.T
12/9/2009
127
142
184
185
2.
622.3483
660.8354
529.9386
794.4046
1242.6820
1319.6562
1586.7940
1586.7946
1242.6819
1319.6568
1586.7940
1586.7940
0.08
-0.45
-0.03
0.39
1
0
0
0
35
0.37
73 6.9e-005
(28)
1.7
65
0.00035
gi|157165660
Mass: 9266
Score: 229
Queries matched: 6
co-chaperonin GroES [Campylobacter concisus 13826]
1
1
1
1
Page 2 of 19
K.TVNISPDEIKK.Y
R.STAAAAAIDSSDLK.I
R.TPSEWQETGIIAGAK.T
R.TPSEWQETGIIAGAK.T
emPAI: 1.62
Query
38
41
120
139
146
218
3.
Observed
465.7479
467.7443
601.3425
650.8585
672.3618
944.0069
Mr(expt)
929.4812
933.4740
1200.6704
1299.7024
1342.7090
1885.9992
Mr(calc)
929.4818
933.4742
1200.6714
1299.7034
1342.7092
1885.9996
-0.59
0
31
1
1
-0.18
0
51
0.011
1
-0.78
1
23
4.4
2
-0.74
0
38
0.15
1
-0.13
1
57 0.0024
1
-0.21
1
29
1.1
1
gi|136429
Mass: 24394
Score: 222
Queries matched: 10
Peptide
K.AVGAEAEGVK.V
-.MNFQPLGK.R
R.VLVERVEETK.T
K.TTASGIIIPDNAK.E
K.AVGAEAEGVKVGEK.V
R.VEETKTTASGIIIPDNAK.E
emPAI: 1.16
Query
16
17
18
70
77
81
173
230
231
232
Observed
421.7582
421.7582
421.7583
523.2853
532.2579
539.2656
763.8799
1106.0550
737.7060
737.7066
Mr(expt)
841.5018
841.5018
841.5020
1044.5560
1062.5012
1076.5166
1525.7452
2210.0954
2210.0962
2210.0980
Mr(calc)
841.5022
841.5022
841.5022
1044.5564
1062.5016
1076.5172
1525.7446
2210.0967
2210.0967
2210.0967
-0.36
0
56 0.0042
1
-0.36
0
(55) 0.0046
1
-0.12
0
(33)
0.72
1
-0.29
0
29
1.7
1
-0.29
0
(30)
1.2
1
-0.51
0
33
0.63
1
0.41
0
45
0.035
1
-0.58
0
60 0.0011
1
-0.25
0
(52) 0.0076
1
0.56
0
(38)
0.18
1
Peptide
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
K.LSSPATLNSR.V
K.APVLSDSSCK.S + Carbamidomethyl (C)
K.APVLSDSSCK.S + Acrylamide (C)
gi|2914482
Mass: 23460
Score: 222
Queries matched: 10
gi|3318722
Mass: 23457
Score: 222
Queries matched: 10
gi|110590762
Mass: 23458
Score: 222
Queries matched: 10
gi|242253868
Mass: 25865
Score: 222
Queries matched: 10
4.
gi|229552
albumin
Mass: 66088
Score: 211
Queries matched: 6
emPAI: 0.21
Query
57
62
101
109
195
196
Observed
501.7951
507.8132
571.8607
582.3186
547.3173
820.4728
Mr(expt)
1001.5756
1013.6118
1141.7068
1162.6226
1638.9301
1638.9310
Mr(calc)
1001.5757
1013.6121
1141.7070
1162.6234
1638.9305
1638.9305
-0.07
0
49
0.01
1
-0.22
0
43
0.024
1
-0.16
1
35
0.056
1
-0.62
0
55 0.0035
1
-0.25
1
28
0.52
1
0.35
1
(24)
1.1
1
Peptide
K.KQTALVELLK.H
K.LVNELTEFAK.T
R.KVPQVSTPTLVEVSR.S
R.KVPQVSTPTLVEVSR.S
gi|1351907
Mass: 69248
Score: 211
Queries matched: 6
gi|30794280
Mass: 69278
Score: 211
Queries matched: 6
gi|74267962
Mass: 69190
Score: 211
Queries matched: 6
gi|154425704
Mass: 69248
Score: 211
Queries matched: 6
5.
gi|6729922
Mass: 14639
Score: 194
Queries matched: 7
emPAI: 0.23
Query
4
15
35
158
159
160
Observed
362.2289
419.7188
454.2532
734.3333
734.3334
734.3339
Mr(expt)
722.4432
837.4230
906.4918
1466.6520
1466.6522
1466.6532
Mr(calc)
722.4439
837.4232
906.4923
1466.6525
1466.6525
1466.6525
-0.91
0
36
0.098
1
-0.20
0
23
6.9
1
-0.53
0
35
0.46
1
-0.33
0
67 0.00016
1
-0.19
0
(32)
0.53
1
0.49
0
(52)
0.004
1
Peptide
K.LVHVQK.W
K.NTEISFK.L
K.SLGVGFATR.Q
K.LGVEFDETTADDR.K
K.LGVEFDETTADDR.K
K.LGVEFDETTADDR.K
12/9/2009
187
798.3812
1594.7478
1594.7475
0.23
1
33
0.46
1
Page 3 of 19
K.LGVEFDETTADDRK.V
Mass: 14770
Score: 194
Queries matched: 7
gi|27805809
fatty acid binding protein 3 [Bos taurus]
Mass: 14753
Score: 194
Queries matched: 7
gi|39939390
fatty acid-binding protein [Capra hircus]
gi|224760716
Mass: 14784
Score: 194
Queries matched: 7
heart fatty acid-binding protein [Bos taurus]
6.
gi|2136760
Mass: 44735
Score: 190
Queries matched: 7
emPAI: 0.15
Query
14
63
75
117
154
155
156
Observed
419.7186
508.2982
530.7689
597.3248
480.5733
720.3566
720.3567
Mr(expt)
837.4226
1014.5818
1059.5232
1192.6350
1438.6981
1438.6986
1438.6988
Mr(calc)
837.4232
1014.5822
1059.5237
1192.6353
1438.6993
1438.6993
1438.6993
-0.66
0
22
8.2
1
-0.35
0
63 0.00058
1
-0.38
0
25
3.8
1
-0.21
0
40
0.13
1
-0.88
0
(28)
1.6
1
-0.48
0
(38)
0.15
1
-0.34
0
39
0.12
1
Peptide
R.AGSAEYLK.T
R.VTGIITQGAR.D
K.QITASSYYK.T
R.QFQFIQVAGR.S
R.DFGHIQYVAAYR.V
R.DFGHIQYVAAYR.V
R.DFGHIQYVAAYR.V
Mass: 47380
Score: 190
Queries matched: 7
gi|2494285
gi|2652932
Mass: 45502
Score: 190
Queries matched: 7
gi|41386719
Mass: 47471
Score: 190
Queries matched: 7
gi|74354284
Mass: 47831
Score: 190
Queries matched: 7
7.
gi|157165021
Mass: 11603
Score: 184
thioredoxin [Campylobacter concisus 13826]
Queries matched: 5
emPAI: 1.17
Query
20
124
163
167
188
8.
Observed
426.7083
608.3506
745.3685
753.3666
800.4166
Mr(expt)
851.4020
1214.6866
1488.7224
1504.7186
1598.8186
gi|2266432
Mass: 59208
Mr(calc)
851.4025
1214.6870
1488.7242
1504.7191
1598.8192
ppm Miss Score
Expect Rank
-0.53
0
25
3.4
1
-0.31
1
35
0.22
1
-1.20
0
(63) 0.00056
1
-0.33
0
75 3.5e-005
1
-0.33
1
49
0.02
1
Score: 152
Queries matched: 6
Peptide
K.ENFDVTK.E
K.QALTDKLNSLL.K.NGELVEQMVGAQSK.Q
K.NGELVEQMVGAQSK.Q + Oxidation (M)
K.YIELTKENFDVTK.E
emPAI: 0.24
Query
144
145
201
202
203
204
Observed
663.3480
671.3448
560.9684
840.9491
560.9686
840.9495
Mr(expt)
1324.6814
1340.6750
1679.8834
1679.8836
1679.8840
1679.8844
Mr(calc)
1324.6809
1340.6758
1679.8842
1679.8842
1679.8842
1679.8842
ppm Miss Score
Expect Rank
0.41
0
82 1.2e-005
1
-0.58
0
(40)
0.092
1
-0.52
0
(32)
0.53
1
-0.36
0
(50)
0.0081
1
-0.16
0
(31)
0.62
1
0.12
0
71 6.5e-005
1
Peptide
K.VAALGSDPHISMK.V
K.VAALGSDPHISMK.V + Oxidation (M)
gi|3183510
Mass: 59239
Score: 152
Queries matched: 6
gi|12647311
Mass: 32265
Score: 152
Queries matched: 6
gi|27806233
Mass: 59225
Score: 152
Queries matched: 6
9.
gi|2232299
Mass: 47885
Score: 126
Queries matched: 3
emPAI: 0.22
Query
157
233
234
Observed
727.3530
740.7217
1110.5790
Mr(expt)
1452.6914
2219.1433
2219.1434
Mr(calc)
1452.6919
2219.1434
2219.1434
ppm Miss Score
Expect Rank
-0.30
0
37
0.25
1
-0.06
0
(46)
0.026
1
0.02
0
90 1.1e-006
1
Peptide
K.SSLICQATDFSPK.Q + Carbamidomethyl (C)
Mass: 48183
Score: 126
Queries matched: 3
gi|3834667
12/9/2009
gi|24496448
immunoglobulin
gi|33413902
immunoglobulin
gi|94981786
immunoglobulin
10.
Page 4 of 19
Mass: 65621
Score: 126
Queries matched: 3
Mass: 49106
Score: 126
Queries matched: 3
Mass: 21826
Score: 126
Queries matched: 3
heavy chain [Bos taurus]
gi|28592070
Mass: 49551
Score: 114
Queries matched: 3
IgM heavy chain constant region, secretory form [Bos taurus]
emPAI: 0.14
Query
157
235
236
Observed
727.3530
745.3936
1117.5870
Mr(expt)
1452.6914
2233.1590
2233.1594
Mr(calc)
1452.6919
2233.1590
2233.1590
ppm Miss Score
Expect Rank
-0.30
0
37
0.25
1
-0.03
0
(23)
3.8
1
0.18
0
77 1.6e-005
1
Peptide
K.SSLICQATDFSPK.Q + Carbamidomethyl (C)
Mass: 52023
Score: 114
Queries matched: 3
gi|28592071
IgM heavy chain constant region, membrane form [Bos taurus]
gi|34538498
Mass: 49909
Score: 114
Queries matched: 3
gi|94981788
Mass: 21762
Score: 114
Queries matched: 3
immunoglobulin heavy chain [Bos taurus]
11.
gi|15673061
Mass: 11430
Score: 106
Queries matched: 3
emPAI: 0.69
50S ribosomal protein L21 [Lactococcus lactis subsp. lactis Il1403]
Query
131
199
200
Observed
626.3268
827.4558
551.9732
Mr(expt)
1250.6390
1652.8970
1652.8978
Mr(calc)
1250.6394
1652.8985
1652.8985
-0.30
0
56 0.0021
1
-0.89
0
50 0.0063
1
-0.45
0
(28)
1.2
1
Peptide
K.VEEGSVIYVEK.L
K.VGAPLVEGATVVGEVEK.H
K.VGAPLVEGATVVGEVEK.H
Mass: 11416
Score: 106
Queries matched: 3
gi|116511920
50S ribosomal protein L21 [Lactococcus lactis subsp. cremoris SK11]
12.
gi|122411
Mass: 15236
Score: 100
Queries matched: 3
Query
73
103
170
Observed
529.2728
573.3297
758.3632
Mr(expt)
1056.5310
1144.6448
1514.7118
Mr(calc)
1056.5314
1144.6452
1514.7113
-0.35
0
31
0.72
1
-0.28
1
35
0.28
1
0.33
0
33
0.39
1
Peptide
R.MFLGFPTTK.T + Oxidation (M)
M.VLSAADKTNVK.A
K.VGGHAGEYGAEALER.M
gi|230479
Mass: 15105
Score: 100
Queries matched: 3
Chain A, Three Dimensional Fourier Synthesis Of Horse Deoxyhaemoglobin At 2.8 Angstroms Resolution
Mass: 15105
Score: 100
Queries matched: 3
gi|230632
Chain A, The Structure Of Horse Methaemoglobin At 2.0 Angstroms Resolution
gi|313500
Mass: 15236
Score: 100
Queries matched: 3
alpha 2 globin [Equus caballus]
gi|24158717
Mass: 15105
Score: 100
Queries matched: 3
Chain A, Crystal Structure Of Horse Carbonmonoxyhemoglobin- Bezafibrate Complex At 1.55a Resolution: A Novel Allosteric Binding Site In R-State Hemog
13.
gi|9725
Mass: 17837
Score: 96
Queries matched: 5
emPAI: 0.19
Query
80
171
172
211
212
Observed
534.3138
508.5982
762.3944
894.4662
894.4674
Mr(expt)
1066.6130
1522.7728
1522.7742
1786.9178
1786.9202
Mr(calc)
1066.6135
1522.7740
1522.7740
1786.9200
1786.9200
-0.41
0
28
0.84
2
-0.78
1
(23)
7.8
1
0.19
1
24
4.5
1
-1.21
0
44
0.048
1
0.13
0
(43) 0.072
1
gi|158769
Mass: 8428
Score: 96
Queries
ubiquitin
gi|158771
Mass: 8543
Score: 96
Queries
ubiquitin
gi|208560
Mass: 8516
Score: 96
Queries
synthetic ubiquitin
gi|208891
Mass: 12690
Score: 96
Queries
beta-galactosidase/ubiquitin fusion protein
gi|229532
Mass: 8446
Score: 96
Queries
ubiquitin
Peptide
K.ESTLHLVLR.L
K.IQDKEGIPPDQQR.L
K.IQDKEGIPPDQQR.L
matched: 5
matched: 5
matched: 5
matched: 5
matched: 5
12/9/2009
Page 5 of 19
gi|323230
Mass: 445804
Score: 96
Queries matched: 5
polyprotein [Bovine viral diarrhea virus strain Osloss]
gi|340062
Mass: 17434
Score: 96
Queries matched: 5
pro-ubiquitin
gi|348148
Mass: 9076
Score: 96
Queries matched: 5
ubiquitin
gi|463375
Mass: 8572
Score: 96
Queries matched: 5
ubiquitin
Mass: 11806
Score: 96
Queries matched: 5
gi|554564
beta-galactosidase/ubiquitin fusion protein
Mass: 18117
Score: 96
Queries matched: 5
gi|600539
ubiquitin [synthetic construct]
gi|1050930
Mass: 11234
Score: 96
Queries matched: 5
gi|1167510
Mass: 14167
Score: 96
Queries matched: 5
TI-225 [Mus musculus]
gi|1421797
Mass: 8932
Score: 96
Queries matched: 5
polyubiquitin
gi|2149467
Mass: 558548
Score: 96
Queries matched: 5
predicted to encode 4983 amino acids with a predicted molecular mass of 558 kDa; ILLC-BVDV ORF [pestivirus type 1]
gi|4506713
Mass: 17953
Score: 96
Queries matched: 5
ubiquitin and ribosomal protein S27a precursor [Homo sapiens]
gi|4507761
Mass: 14719
Score: 96
Queries matched: 5
ubiquitin and ribosomal protein L40 precursor [Homo sapiens]
gi|5523975
Mass: 34352
Score: 96
Queries matched: 5
polyprotein [Bovine viral diarrhea virus genotype 2]
Mass: 28945
Score: 96
Queries matched: 5
gi|5523983
gi|5822011
Mass: 8614
Score: 96
Queries matched: 5
Chain B, Structural Basis For The Specificity Of Ubiquitin C- Terminal Hydrolases
gi|11528469
Mass: 64092
Score: 96
Queries matched: 5
humanized L1/ubiqutin hybrid protein [synthetic construct]
gi|12240039
Mass: 14664
Score: 96
Queries matched: 5
ubiquitin [Biomphalaria glabrata]
gi|13021896
Mass: 62384
Score: 96
Queries matched: 5
humanized ubiquitin/L1 delta/H-2 Db CTL epitope hybrid protein [synthetic construct]
Mass: 8673
Score: 96
Queries matched: 5
gi|13172895
polyubiquitin GmUblast [Galleria mellonella]
gi|13195690
Mass: 17939
Score: 96
Queries matched: 5
ribosomal protein S27a [Mus musculus]
Mass: 16165
Score: 96
Queries matched: 5
gi|13569612
ubiquitin A-52 residue ribosomal protein fusion product 1 [Homo sapiens]
gi|15187109
Mass: 14738
Score: 96
Queries matched: 5
ubiquitin [Branchiostoma belcheri]
gi|15293953
Mass: 14775
Score: 96
Queries matched: 5
ribosomal protein L40 [Ictalurus punctatus]
Mass: 14720
Score: 96
Queries matched: 5
gi|17136570
ribosomal protein L40 [Drosophila melanogaster]
gi|17136574
Mass: 17929
Score: 96
Queries matched: 5
ribosomal protein S27A [Drosophila melanogaster]
gi|18071662
Mass: 17995
Score: 96
Queries matched: 5
ubiquitin/ribosomal protein S27a fusion protein [Branchiostoma belcheri tsingtaunese]
gi|18253057
Mass: 17962
Score: 96
Queries matched: 5
ribosomal protein S27A [Spodoptera frugiperda]
gi|20069096
Mass: 14046
Score: 96
Queries matched: 5
ubiquitin/ribosomal L40 fusion protein [Aplysia californica]
gi|20530720
Mass: 17997
Score: 96
Queries matched: 5
40s ribosomal protein S27a [Epinephelus coioides]
Mass: 16603
Score: 96
Queries matched: 5
gi|28189426
similar to ubiquitin-S27a fusion protein [Bos taurus]
gi|29169226
Mass: 12257
Score: 96
Queries matched: 5
polyubiquitin [Camelus dromedarius]
gi|29612581
Mass: 19698
Score: 96
Queries matched: 5
gi|39725569
Mass: 335187
Score: 96
Queries matched: 5
poryprotein [Bovine viral diarrhea virus 190cp]
Mass: 269050
Score: 96
Queries matched: 5
gi|39725571
poryprotein [Bovine viral diarrhea virus T-20]
gi|39840928
Mass: 17853
Score: 96
Queries matched: 5
ubiquitin [Antheraea yamamai]
gi|41055516
Mass: 17988
Score: 96
Queries matched: 5
ribosomal protein S27a [Danio rerio]
Mass: 17972
Score: 96
Queries matched: 5
gi|45934569
ribosomal protein S27a [Aedes aegypti]
gi|46811235
Mass: 13359
Score: 96
Queries matched: 5
40S ribosomal protein S27A [Pseudopleuronectes americanus]
gi|47027862
Mass: 10192
Score: 96
Queries matched: 5
ubq-S27a protein [Ovis aries]
gi|47217408
Mass: 14736
Score: 96
Queries matched: 5
unnamed protein product [Tetraodon nigroviridis]
gi|47604951
Mass: 17968
Score: 96
Queries matched: 5
ribosomal protein S27a [Gallus gallus]
12/9/2009
Page 6 of 19
gi|48425521
Mass: 8540
Score: 96
Queries matched: 5
Chain B, Tsg101(Uev) Domain In Complex With Ubiquitin
gi|49532928
Mass: 17936
Score: 96
Queries matched: 5
Ribosomal protein S27A [Plutella xylostella]
gi|50284394
Mass: 17837
Score: 96
Queries matched: 5
ubiquitin/ribosomal protein S27Ae fusion protein [Papilio dardanus]
gi|50344522
Mass: 17838
Score: 96
Queries matched: 5
ubiquitin/S27Ae ribosomal protein [Carabus granulatus]
Mass: 8578
Score: 96
Queries matched: 5
gi|51701909
Mass: 8560
Score: 96
Queries matched: 5
gi|51701919
gi|53987035
Mass: 14148
Score: 96
Queries matched: 5
poly-histidine-tagged ubiquitin [Cloning vector pHUE]
gi|55228560
Mass: 14739
Score: 96
Queries matched: 5
ubuiquitin/ribosomal L40 fusion protein [Scleronephthya gracillimum]
gi|56078799
Mass: 17893
Score: 96
Queries matched: 5
Ribosomal protein S27a [Homo sapiens]
gi|56199434
Mass: 18613
Score: 96
Queries matched: 5
unknown [Culicoides sonorensis]
gi|56967061
Mass: 8503
Score: 96
Queries matched: 5
Chain B, Crystal Structure Of Uchl3-Ubvme Complex
gi|61378584
Mass: 14747
Score: 96
Queries matched: 5
ubiquitin/ribosomal fusion protein [Bubalus bubalis]
gi|61741081
Mass: 16173
Score: 96
Queries matched: 5
ubiquitin/ribosomal 27a [Marsupenaeus japonicus]
Mass: 11036
Score: 96
Queries matched: 5
gi|66361226
Chain B, Solution Structure Of S5a Uim-1UBIQUITIN COMPLEX
gi|66362872
Mass: 15100
Score: 96
Queries matched: 5
60S ribosomal protein L40 [Cryptosporidium parvum Iowa II]
gi|67083981
Mass: 17924
Score: 96
Queries matched: 5
ubiquitin/ribosomal protein S27a fusion protein [Ixodes scapularis]
gi|67614873
Mass: 14658
Score: 96
Queries matched: 5
ubiquitin / ribosomal protein CEP52 [Cryptosporidium hominis TU502]
gi|69608566
Mass: 17951
Score: 96
Queries matched: 5
ubiquitin/ribosomal protein S27Ae fusion protein [Agriotes lineatus]
Mass: 17879
Score: 96
Queries matched: 5
gi|69608583
ubiquitin/ribosomal protein S27Ae fusion protein [Eucinetus sp. APV-2005]
gi|69608587
Mass: 17854
Score: 96
Queries matched: 5
ubiquitin/ribosomal protein S27Ae fusion protein [Micromalthus debilis]
Mass: 17880
Score: 96
Queries matched: 5
gi|69608591
ubiquitin/ribosomal protein S27Ae fusion protein [Timarcha balearica]
gi|70909907
Mass: 14693
Score: 96
Queries matched: 5
ribosomal protein Ubq/L40e [Agriotes lineatus]
gi|70909913
Mass: 14679
Score: 96
Queries matched: 5
ribosomal protein Ubq/L40e [Dascillus cervinus]
Mass: 14887
Score: 96
Queries matched: 5
gi|71033899
ubiquitin/ribosomal fusion protein [Theileria parva strain Muguga]
gi|71042034
Mass: 8542
Score: 96
Queries matched: 5
Chain A, X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin
gi|71042037
Score: 96
Queries matched: 5
gi|72172080
Mass: 9552
Score: 96
Queries matched: 5
polyubiquitin [Ictalurus punctatus]
Mass: 26177
Score: 96
Queries matched: 5
gi|73970166
PREDICTED: similar to ubiquitin and ribosomal protein S27a precursor [Canis familiaris]
Mass: 7199
Score: 96
Queries matched: 5
gi|78099807
Mass: 14702
Score: 96
Queries matched: 5
gi|80751117
hypothetical protein LOC612529 [Canis lupus familiaris]
gi|80751129
Mass: 14747
Score: 96
Queries matched: 5
ubiquitin A-52 residue ribosomal protein fusion product 1 [Danio rerio]
Mass: 14869
Score: 96
Queries matched: 5
gi|84997964
ubiquitin/60S ribosomal fusion protein [Theileria annulata]
gi|85719967
Mass: 12889
Score: 96
Queries matched: 5
ubiquitin and ribosomal protein S27a precursor [Ictalurus punctatus]
gi|90819968
Mass: 17864
Score: 96
Queries matched: 5
putative ubiquitin/ribosomal protein S27Ae fusion protein [Graphocephala atropunctata]
gi|91077776
Mass: 17822
Score: 96
Queries matched: 5
PREDICTED: similar to ubiquitin/S27Ae ribosomal protein [Tribolium castaneum]
gi|91080141
Mass: 14750
Score: 96
Queries matched: 5
PREDICTED: similar to ribosomal protein Ubq/L40e [Tribolium castaneum]
gi|94369204
Mass: 8723
Score: 96
Queries matched: 5
PREDICTED: hypothetical protein [Mus musculus]
gi|94385661
Mass: 14657
Score: 96
Queries matched: 5
PREDICTED: similar to ubiquitin A-52 residue ribosomal protein fusion product 1 [Mus musculus]
gi|109121596
Mass: 14763
Score: 96
Queries matched: 5
gi|109124042
Mass: 19555
Score: 96
Queries matched: 5
gi|109157803
Mass: 9016
Score: 96
Queries matched: 5
Chain A, Crystal Structure Of The 9-10 8 Glycine Insertion Mutant Of Ubiquitin.
gi|109157805
Mass: 9341
Score: 96
Queries matched: 5
12/9/2009
Page 7 of 19
Chain A, Crystal Structure Of The 9-10 Moad Insertion Mutant Of Ubiquitin
gi|110671470
Mass: 14795
Score: 96
Queries matched: 5
putative ubiquitin/ribosomal fusion protein [Diaphorina citri]
gi|113431898
Mass: 15448
Score: 96
Queries matched: 5
ubiquitin/ribosomal fusion protein homologue [Salmo salar]
gi|114577583
Mass: 25695
Score: 96
Queries matched: 5
PREDICTED: similar to RPS27A protein [Pan troglodytes]
gi|114662918
Mass: 17926
Score: 96
Queries matched: 5
PREDICTED: similar to ubiquitin isoform 1 [Pan troglodytes]
gi|114676157
Mass: 24353
Score: 96
Queries matched: 5
PREDICTED: similar to ubiquitin A-52 residue ribosomal protein fusion product 1 [Pan troglodytes]
gi|116488178
Mass: 8429
Score: 96
Queries matched: 5
polyubiquitin [Scophthalmus maximus]
gi|119624908
Mass: 8663
Score: 96
Queries matched: 5
ubiquitin B, isoform CRA_b [Homo sapiens]
gi|119624910
Mass: 13066
Score: 96
Queries matched: 5
ubiquitin B, isoform CRA_d [Homo sapiens]
Mass: 8629
Score: 96
Queries matched: 5
gi|119909248
PREDICTED: similar to ubiquitin B [Bos taurus]
gi|121543853
Mass: 17854
Score: 96
Queries matched: 5
putative ubiquitin/ribosomal protein S27Ae fusion protein [Maconellicoccus hirsutus]
gi|123316118
Mass: 17772
Score: 96
Queries matched: 5
ubiquitin [Portunus pelagicus]
Mass: 14733
Score: 96
Queries matched: 5
gi|124300855
ubiquitin [Solea senegalensis]
gi|125742521
Mass: 17875
Score: 96
Queries matched: 5
putative ubiquitin/ribosomal protein S27Ae fusion protein [Oncopeltus fasciatus]
gi|126303240
Mass: 18001
Score: 96
Queries matched: 5
PREDICTED: similar to ubiquitin [Monodelphis domestica]
gi|126335870
Mass: 12246
Score: 96
Queries matched: 5
PREDICTED: similar to putative ubiquitin/ribosomal fusion protein [Monodelphis domestica]
gi|134105063
Mass: 8571
Score: 96
Queries matched: 5
Chain B, Crystal Structure And Solution Nmr Studies Of Lys48-Linked Tetraubiquitin At Neutral Ph
gi|134105065
Mass: 8616
Score: 96
Queries matched: 5
Chain D, Crystal Structure And Solution Nmr Studies Of Lys48-Linked Tetraubiquitin At Neutral Ph
gi|148688761
Mass: 10235
Score: 96
Queries matched: 5
gi|148708840
Mass: 14662
Score: 96
Queries matched: 5
gi|149044848
Mass: 16414
Score: 96
Queries matched: 5
Mass: 8697
Score: 96
Queries matched: 5
gi|149052926
Mass: 12424
Score: 96
Queries matched: 5
gi|149242629
Chain A, Structure Of Ubiquitin Solved By Sad Using The Lanthanide- Binding Tag
gi|149287148
Mass: 17968
Score: 96
Queries matched: 5
ubiquitin/40S ribosomal protein S27a [Ornithodoros parkeri]
gi|149639066
Mass: 24664
Score: 96
Queries matched: 5
PREDICTED: similar to ubiquitin A-52 residue ribosomal protein fusion product 1 [Ornithorhynchus anatinus]
gi|152013697
Mass: 14794
Score: 96
Queries matched: 5
ubiquitin/ribosomal L40 fusion protein [Artemia franciscana]
gi|152925970
Mass: 14702
Score: 96
Queries matched: 5
ubiquitin fusion protein [Cyprinus carpio]
Mass: 14775
Score: 96
Queries matched: 5
gi|156083999
ubiquitin / ribosomal protein CEP52 [Babesia bovis T2Bo]
gi|156396888
Mass: 16792
Score: 96
Queries matched: 5
gi|156405092
Mass: 17968
Score: 96
Queries matched: 5
gi|157136377
Mass: 17986
Score: 96
Queries matched: 5
ubiquitin (ribosomal protein L40) [Aedes aegypti]
Mass: 14694
Score: 96
Queries matched: 5
gi|157361519
ubiquitin/ribosomal L40 fusion protein-like protein [Phlebotomus papatasi]
gi|159145744
Mass: 14669
Score: 96
Queries matched: 5
putative ribosomal protein L40 [Barentsia elongata]
gi|159163685
Mass: 8588
Score: 96
Queries matched: 5
Chain B, Solution Structure Of The Human Mms2-Ubiquitin Complex
gi|161661039
Mass: 17995
Score: 96
Queries matched: 5
60S ribosomal protein L40A [Lycosa singoriensis]
gi|163915892
Mass: 9527
Score: 96
Queries matched: 5
LOC100135402 protein [Xenopus tropicalis]
gi|169146693
Mass: 9909
Score: 96
Queries matched: 5
gi|172051100
Mass: 17106
Score: 96
Queries matched: 5
ubiquitin/40S ribosomal protein S27a [Ornithodoros coriaceus]
gi|185134441
Mass: 14733
Score: 96
Queries matched: 5
ubiquitin [Oncorhynchus mykiss]
gi|188572494
Mass: 17982
Score: 96
Queries matched: 5
putative ubiquitin/40S ribosomal protein RPS27A fusion protein [Phoronis muelleri]
gi|194246105
Mass: 17904
Score: 96
Queries matched: 5
ubiquitin/ribosomal protein S27a fusion protein [Dermacentor variabilis]
gi|195116685
Mass: 17915
Score: 96
Queries matched: 5
12/9/2009
Page 8 of 19
gi|195190296
Mass: 8833
Score: 96
Queries matched: 5
gi|195342419
Mass: 14707
Score: 96
Queries matched: 5
gi|195491526
Mass: 8872
Score: 96
Queries matched: 5
gi|195565407
Mass: 11649
Score: 96
Queries matched: 5
gi|196016140
Mass: 11249
Score: 96
Queries matched: 5
expressed hypothetical protein [Trichoplax adhaerens]
gi|197107121
Mass: 8606
Score: 96
Queries matched: 5
Chain B, Cin85 Sh3-C Domain In Complex With Ubiquitin
gi|197632563
Mass: 18002
Score: 96
Queries matched: 5
ribosomal protein S27-3 [Salmo salar]
gi|197725012
Mass: 8588
Score: 96
Queries matched: 5
Chain B, Crystal Structure Of Human Amsh-Lp Dub Domain In Complex With Lys63-Linked Ubiquitin Dimer
Mass: 8675
Score: 96
Queries matched: 5
gi|197725013
Chain C, Crystal Structure Of Human Amsh-Lp Dub Domain In Complex With Lys63-Linked Ubiquitin Dimer
gi|208435490
Mass: 11515
Score: 96
Queries matched: 5
Chain A, Automated Nmr Structure Of The Ubb By Fapsy
gi|208435644
Mass: 8956
Score: 96
Queries matched: 5
Chain X, Crystal Structure Of K63-Specific Fab Apu.3a8 Bound To K63- Linked Di-Ubiquitin
Mass: 8869
Score: 96
Queries matched: 5
gi|208435645
Chain Y, Crystal Structure Of K63-Specific Fab Apu.3a8 Bound To K63- Linked Di-Ubiquitin
gi|209730830
Mass: 14422
Score: 96
Queries matched: 5
gi|209735846
Mass: 14793
Score: 96
Queries matched: 5
gi|209879912
Mass: 14735
Score: 96
Queries matched: 5
60S ribosomal protein L40 [Cryptosporidium muris RN66]
gi|213512766
Mass: 14706
Score: 96
Queries matched: 5
ubiquitin A-52 residue ribosomal protein fusion product 1like 2 [Salmo salar]
gi|219499522
Mass: 13638
Score: 96
Queries matched: 5
gi|223646272
Mass: 8674
Score: 96
Queries matched: 5
gi|223646368
Mass: 14732
Score: 96
Queries matched: 5
gi|223646710
Mass: 14661
Score: 96
Queries matched: 5
Mass: 14653
Score: 96
Queries matched: 5
gi|225703194
Mass: 17972
Score: 96
Queries matched: 5
gi|225705126
gi|225710628
Mass: 14675
Score: 96
Queries matched: 5
gi|225714096
Mass: 14691
Score: 96
Queries matched: 5
gi|225715160
Mass: 10466
Score: 96
Queries matched: 5
gi|226371948
Mass: 17964
Score: 96
Queries matched: 5
Ubiquitin [Rana catesbeiana]
Mass: 7554
Score: 96
Queries matched: 5
gi|228069369
ubiquitin [Oncorhynchus tshawytscha]
gi|229368168
Mass: 17858
Score: 96
Queries matched: 5
Ubiquitin [Anoplopoma fimbria]
gi|240952274
Mass: 19385
Score: 96
Queries matched: 5
40S ribosomal protein S27A, putative [Ixodes scapularis]
gi|158763
Mass: 8558
Score: 95
Queries matched: 5
ubiquitin
Mass: 8535
Score: 95
Queries matched: 5
gi|158765
ubiquitin
gi|158767
Mass: 8540
Score: 95
Queries matched: 5
ubiquitin
gi|323208
Mass: 210031
Score: 95
Queries matched: 5
nonstructural protein; putative helicase/protease; contains duplication; contains ubiquitin-coding region; putative
gi|5523967
Mass: 38347
Score: 95
Queries matched: 5
gi|5523971
Mass: 19929
Score: 95
Queries matched: 5
gi|5523973
Mass: 25792
Score: 95
Queries matched: 5
gi|5523977
Mass: 40614
Score: 95
Queries matched: 5
gi|5523981
Mass: 24092
Score: 95
Queries matched: 5
gi|5523985
Mass: 39550
Score: 95
Queries matched: 5
gi|10121776
Mass: 71690
Score: 95
Queries matched: 5
polyprotein [bovine viral diarrhea virus type 2]
gi|21314339
Mass: 12686
Score: 95
Queries matched: 5
12/9/2009
Page 9 of 19
gi|28189228
Mass: 12897
Score: 95
Queries matched: 5
gi|29425820
Mass: 13038
Score: 95
Queries matched: 5
polyubiquitin [Anas platyrhynchos]
gi|60598636
Mass: 20586
Score: 95
Queries matched: 5
gi|62079638
Mass: 17851
Score: 95
Queries matched: 5
ubiquitin [Oreochromis mossambicus]
gi|83944648
Mass: 13674
Score: 95
Queries matched: 5
ubiquitin [Eisenia fetida]
gi|119569495
Mass: 16522
Score: 95
Queries matched: 5
hCG1993742 [Homo sapiens]
gi|119624911
Mass: 16792
Score: 95
Queries matched: 5
ubiquitin B, isoform CRA_e [Homo sapiens]
gi|149063233
Mass: 20300
Score: 95
Queries matched: 5
Mass: 11646
Score: 95
Queries matched: 5
gi|225704224
gi|1321735
Mass: 15669
Score: 93
Queries matched: 5
ubiquitin [Carabus alpestris]
gi|1805696
Mass: 15038
Score: 93
Queries matched: 5
polyubiquitin [Ceratitis capitata]
Mass: 35289
Score: 93
Queries matched: 5
gi|5523969
gi|5523987
Mass: 44260
Score: 93
Queries matched: 5
gi|26353460
Mass: 17214
Score: 93
Queries matched: 5
gi|54610299
Mass: 17621
Score: 93
Queries matched: 5
polyubiquitin-like protein [Schistosoma japonicum]
gi|82622379
Mass: 17936
Score: 93
Queries matched: 5
unknown [Theileria lestoquardi]
gi|90075190
Mass: 17892
Score: 93
Queries matched: 5
gi|149063235
Mass: 18245
Score: 93
Queries matched: 5
rCG21224 [Rattus norvegicus]
gi|86370988
Mass: 21604
Score: 93
Queries matched: 5
ubiquitin C [Ictalurus punctatus]
gi|225711930
Mass: 25657
Score: 93
Queries matched: 5
Mass: 18420
Score: 92
Queries matched: 5
gi|163575
polyubiquitin
Mass: 17648
Score: 92
Queries matched: 5
gi|212851
ubiquitin polyprotein (heat shock related)
gi|3954791
Mass: 17738
Score: 92
Queries matched: 5
unnamed protein product [Gallus gallus]
gi|5523979
Mass: 48053
Score: 92
Queries matched: 5
gi|7799051
Mass: 17236
Score: 92
Queries matched: 5
ubiquitin [Cyanidium caldarium]
gi|14250448
Mass: 22708
Score: 92
Queries matched: 5
Mass: 17587
Score: 92
Queries matched: 5
gi|28189839
gi|28189917
Mass: 19318
Score: 92
Queries matched: 5
gi|54300702
Mass: 17142
Score: 92
Queries matched: 5
gi|56199552
Mass: 18776
Score: 92
Queries matched: 5
Mass: 17479
Score: 92
Queries matched: 5
gi|71033201
ubiquitin [Theileria parva strain Muguga]
gi|73955932
Mass: 20280
Score: 92
Queries matched: 5
gi|73955936
Mass: 17204
Score: 92
Queries matched: 5
gi|84998672
Mass: 17935
Score: 92
Queries matched: 5
ubiquitin [Theileria annulata]
gi|114152964
Mass: 26493
Score: 92
Queries matched: 5
ubiquitin/40S ribosomal protein S27a fusion protein [Argas monolakensis]
gi|149052896
Mass: 17264
Score: 92
Queries matched: 5
rCG34296, isoform CRA_a [Rattus norvegicus]
gi|197129059
Mass: 21234
Score: 92
Queries matched: 5
gi|197129060
Mass: 17215
Score: 92
Queries matched: 5
gi|209875527
Mass: 17405
Score: 92
Queries matched: 5
ubiquitin family protein [Cryptosporidium muris RN66]
gi|223646258
Mass: 21426
Score: 92
Queries matched: 5
gi|225733991
Mass: 17245
Score: 92
Queries matched: 5
12/9/2009
Page 10 of 19
Chain A, Nemo Cozi Domain Incomplex With Diubiquitin In P212121 Space Group
gi|228311825
Mass: 17081
Score: 92
Queries matched: 5
Chain A, Crystal Structure Of Linear Di-Ubiquitin
gi|256079432
Mass: 17214
Score: 92
Queries matched: 5
gi|124784679
Mass: 28105
Score: 92
Queries matched: 5
polyubiquitin [Taenia asiatica]
gi|567767
Mass: 117979
Score: 91
Queries matched: 5
p125 protein
gi|2118960
Mass: 18789
Score: 91
Queries matched: 5
polyubiquitin - African clawed frog (fragment)
gi|56199554
Mass: 21565
Score: 91
Queries matched: 5
gi|90076064
Mass: 23571
Score: 91
Queries matched: 5
gi|195587566
Mass: 21780
Score: 91
Queries matched: 5
Mass: 25104
Score: 90
Queries matched: 5
gi|49586
unnamed protein product [Cricetulus sp.]
gi|625174
Mass: 25279
Score: 90
Queries matched: 5
ubiquitin [Artemia franciscana]
gi|140083661
Mass: 23510
Score: 90
Queries matched: 5
ubiquitin B [Sus scrofa]
Mass: 24947
Score: 90
Queries matched: 5
gi|164430972
ubiquitin [Drosophila silvestris]
gi|195164961
Mass: 26219
Score: 90
Queries matched: 5
gi|197129049
Mass: 23410
Score: 90
Queries matched: 5
gi|197129052
Mass: 27521
Score: 90
Queries matched: 5
gi|197129065
Mass: 25758
Score: 90
Queries matched: 5
gi|225709684
Mass: 25805
Score: 90
Queries matched: 5
gi|225715800
Mass: 25743
Score: 90
Queries matched: 5
gi|93359417
Mass: 29809
Score: 90
Queries matched: 5
polyubiquitin [Anser anser]
gi|158753
Mass: 25955
Score: 90
Queries matched: 5
ubiquitin
Mass: 27489
Score: 90
Queries matched: 5
gi|163573
polyubiquitin
Mass: 26857
Score: 90
Queries matched: 5
gi|5912028
hypothetical protein [Homo sapiens]
gi|11024714
Mass: 25746
Score: 90
Queries matched: 5
ubiquitin B precursor [Homo sapiens]
gi|12833702
Mass: 25806
Score: 90
Queries matched: 5
gi|37595366
Mass: 26486
Score: 90
Queries matched: 5
gi|55977015
Mass: 25742
Score: 90
Queries matched: 5
Mass: 25859
Score: 90
Queries matched: 5
gi|61369554
ubiquitin B [synthetic construct]
gi|66358028
Mass: 27245
Score: 90
Queries matched: 5
polyubiquitin with 3 Ub domains [Cryptosporidium parvum Iowa II]
gi|67616274
Mass: 25774
Score: 90
Queries matched: 5
ubiquitin B [Cryptosporidium hominis TU502]
gi|73955934
Mass: 25715
Score: 90
Queries matched: 5
Mass: 31250
Score: 90
Queries matched: 5
gi|74214198
gi|109497142
Mass: 31226
Score: 90
Queries matched: 5
PREDICTED: similar to ubiquitin C isoform 1 [Rattus norvegicus]
gi|110671522
Mass: 25790
Score: 90
Queries matched: 5
putative ubiquitin B [Diaphorina citri]
gi|149723670
Mass: 34153
Score: 90
Queries matched: 5
PREDICTED: hypothetical protein [Equus caballus]
gi|156087036
Mass: 26225
Score: 90
Queries matched: 5
polyubiquitin [Babesia bovis T2Bo]
gi|156358391
Mass: 25791
Score: 90
Queries matched: 5
gi|195469924
Mass: 25827
Score: 90
Queries matched: 5
gi|197129056
Mass: 27945
Score: 90
Queries matched: 5
gi|213510698
Mass: 25757
Score: 90
Queries matched: 5
polyubiquitin [Salmo salar]
gi|220808512
Mass: 26603
Score: 90
Queries matched: 5
gi|256072199
Mass: 25643
Score: 90
Queries matched: 5
12/9/2009
Page 11 of 19
gi|164708
Mass: 28741
Score: 89
Queries matched: 5
poly-ubiquitin precursor
gi|340058
Mass: 30285
Score: 89
Queries matched: 5
ubiquitin precursor
gi|12851404
Mass: 34235
Score: 89
Queries matched: 5
gi|89891990
Mass: 30391
Score: 89
Queries matched: 5
ubiquitin C II [Anser anser]
gi|195587564
Mass: 34024
Score: 89
Queries matched: 5
gi|197129051
Mass: 34289
Score: 89
Queries matched: 5
gi|221048065
Mass: 28658
Score: 89
Queries matched: 5
ubiquitin C variant [Epinephelus coioides]
gi|223646966
Mass: 34244
Score: 89
Queries matched: 5
Mass: 34381
Score: 88
Queries matched: 5
gi|12849189
gi|42490818
Mass: 34342
Score: 88
Queries matched: 5
gi|104303708
Mass: 34321
Score: 88
Queries matched: 5
ubiquitin [Rana nigromaculata]
Mass: 34393
Score: 88
Queries matched: 5
gi|444791
ubiquitin
gi|18044723
Mass: 34356
Score: 88
Queries matched: 5
gi|194043617
Mass: 34332
Score: 88
Queries matched: 5
PREDICTED: ubiquitin C isoform 1 [Sus scrofa]
gi|1197093
Mass: 34962
Score: 88
Queries matched: 5
ubiquitin [Cavia porcellus]
gi|4105408
Mass: 33123
Score: 88
Queries matched: 5
polyubiquitin [Schistosoma mansoni]
gi|6755919
Mass: 34348
Score: 88
Queries matched: 5
gi|15928840
Mass: 34284
Score: 88
Queries matched: 5
gi|19483857
Mass: 39791
Score: 88
Queries matched: 5
gi|21428336
Mass: 34369
Score: 88
Queries matched: 5
GH17513p [Drosophila melanogaster]
Mass: 34347
Score: 88
Queries matched: 5
gi|27806505
polyubiquitin [Bos taurus]
Mass: 34305
Score: 88
Queries matched: 5
gi|57163957
polyubiquitin [Ovis aries]
gi|89994036
Mass: 34287
Score: 88
Queries matched: 5
Polyubiquitin [Bos taurus]
gi|126352454
Mass: 34321
Score: 88
Queries matched: 5
ubiquitin C [Equus caballus]
gi|185135461
Mass: 34298
Score: 88
Queries matched: 5
polyubiquitin [Oncorhynchus mykiss]
gi|194944118
Mass: 36863
Score: 88
Queries matched: 5
Mass: 34458
Score: 88
Queries matched: 5
gi|195170771
gi|195340400
Mass: 35980
Score: 88
Queries matched: 5
gi|195403389
Mass: 43087
Score: 88
Queries matched: 5
gi|195491528
Mass: 35822
Score: 88
Queries matched: 5
Mass: 34370
Score: 88
Queries matched: 5
gi|197129053
gi|221222312
Mass: 34312
Score: 88
Queries matched: 5
gi|223646702
Mass: 34346
Score: 88
Queries matched: 5
gi|256079430
Mass: 39365
Score: 88
Queries matched: 5
gi|30060222
Mass: 38183
Score: 87
Queries matched: 5
polyubiquitin [Schistosoma japonicum]
gi|16552475
Mass: 43622
Score: 87
Queries matched: 5
gi|56754831
Mass: 42822
Score: 87
Queries matched: 5
gi|197129055
Mass: 45883
Score: 87
Queries matched: 5
gi|197129067
Mass: 42829
Score: 87
Queries matched: 5
gi|2118958
Mass: 42889
Score: 86
Queries matched: 5
polyubiquitin 5 - Chinese hamster
gi|73695428
Mass: 48309
Score: 86
Queries matched: 5
12/9/2009
Page 12 of 19
gi|73995130
Mass: 42825
Score: 86
Queries matched: 5
PREDICTED: similar to CG11624-PA, isoform A [Canis familiaris]
gi|148237866
Mass: 42726
Score: 86
Queries matched: 5
similar to ubiquitin C [Xenopus laevis]
gi|149535948
Mass: 42829
Score: 86
Queries matched: 5
PREDICTED: similar to ubiquitin [Ornithorhynchus anatinus]
gi|195164963
Mass: 42910
Score: 86
Queries matched: 5
gi|209738556
Mass: 42823
Score: 86
Queries matched: 5
gi|112983984
Mass: 102361
Score: 86
Queries matched: 5
polyubiquitin [Bombyx mori]
gi|157420221
Mass: 48667
Score: 86
Queries matched: 5
Ubc protein [Penaeus monodon]
gi|29612653
Mass: 54095
Score: 85
Queries matched: 5
Mass: 51381
Score: 85
Queries matched: 5
gi|56753927
SJCHGC00176 protein [Schistosoma japonicum]
gi|169146689
Mass: 51610
Score: 85
Queries matched: 5
gi|189441656
Mass: 51470
Score: 85
Queries matched: 5
Mass: 51431
Score: 85
Queries matched: 5
gi|195399337
gi|196016942
Mass: 51478
Score: 85
Queries matched: 5
gi|223646766
Mass: 51365
Score: 85
Queries matched: 5
gi|118150620
Mass: 59884
Score: 85
Queries matched: 5
hypothetical protein LOC777766 [Danio rerio]
gi|24640086
Mass: 59993
Score: 84
Queries matched: 5
Ubiquitin-5E [Drosophila melanogaster]
gi|38114661
Mass: 61486
Score: 84
Queries matched: 5
gi|109099227
Mass: 63427
Score: 84
Queries matched: 5
PREDICTED: similar to Ubiquitin-63E CG11624-PA, isoform A [Macaca mulatta]
gi|149254469
Mass: 65416
Score: 84
Queries matched: 5
PREDICTED: similar to Ubc protein isoform 1 [Mus musculus]
gi|156358687
Mass: 59923
Score: 84
Queries matched: 5
Mass: 60127
Score: 84
Queries matched: 5
gi|195011548
Mass: 59908
Score: 84
Queries matched: 5
gi|213982779
hypothetical protein LOC100216108 [Xenopus (Silurana) tropicalis]
gi|148687613
Mass: 69069
Score: 84
Queries matched: 5
ubiquitin C, isoform CRA_a [Mus musculus]
gi|1304128
Mass: 68681
Score: 84
Queries matched: 5
polyubiquitin [Homo sapiens]
gi|158256510
Mass: 68406
Score: 84
Queries matched: 5
gi|195042539
Mass: 78569
Score: 84
Queries matched: 5
Mass: 67453
Score: 84
Queries matched: 5
gi|219418415
gi|940395
Mass: 73905
Score: 84
Queries matched: 5
polyubiquitin [Cricetulus sp.]
gi|18256864
Mass: 73958
Score: 84
Queries matched: 5
gi|47682711
Mass: 70128
Score: 84
Queries matched: 5
Mass: 68450
Score: 84
Queries matched: 5
gi|55742507
ubiquitin C [Xenopus (Silurana) tropicalis]
gi|58618886
Mass: 73675
Score: 84
Queries matched: 5
gi|145046250
Mass: 68553
Score: 84
Queries matched: 5
gi|148235355
Mass: 68464
Score: 84
Queries matched: 5
ubiquitin C [Xenopus laevis]
gi|157138589
Mass: 68479
Score: 84
Queries matched: 5
gi|194762588
Mass: 68535
Score: 84
Queries matched: 5
gi|195132655
Mass: 68465
Score: 84
Queries matched: 5
gi|195137906
Mass: 74931
Score: 84
Queries matched: 5
gi|195448336
Mass: 68569
Score: 84
Queries matched: 5
GK10077 [Drosophila willistoni]
gi|510473
Mass: 78339
Score: 83
Queries matched: 5
polyubiquitin [Artemia franciscana]
gi|9837284
Mass: 82489
Score: 83
Queries matched: 5
12/9/2009
Page 13 of 19
gi|67191208
Mass: 76982
Score: 83
Queries matched: 5
ubiquitin C [Homo sapiens]
gi|76638716
Mass: 77523
Score: 83
Queries matched: 5
PREDICTED: ubiquitin C isoform 12 [Bos taurus]
gi|340068
Mass: 76992
Score: 83
Queries matched: 5
ubiquitin
gi|46359622
Mass: 77007
Score: 83
Queries matched: 5
polyubiquitin [Crassostrea gigas]
gi|51873890
Mass: 78650
Score: 83
Queries matched: 5
Unknown (protein for IMAGE:2822684) [Homo sapiens]
gi|86515356
Mass: 76994
Score: 83
Queries matched: 5
gi|157671923
Mass: 82499
Score: 83
Queries matched: 5
ubiquitin C [Mus musculus]
gi|8394502
Mass: 91031
Score: 82
Queries matched: 5
ubiquitin C [Rattus norvegicus]
Mass: 85746
Score: 82
Queries matched: 5
gi|24657014
Ubiquitin-63E, isoform A [Drosophila melanogaster]
gi|126314283
Mass: 93785
Score: 82
Queries matched: 5
PREDICTED: similar to ubiquitin [Monodelphis domestica]
gi|157138587
Mass: 85562
Score: 82
Queries matched: 5
Mass: 85533
Score: 82
Queries matched: 5
gi|208022675
ubiquitin C [Pan troglodytes]
gi|9837286
Mass: 99515
Score: 82
Queries matched: 5
gi|194749344
Mass: 94089
Score: 82
Queries matched: 5
gi|2627131
Mass: 99531
Score: 81
Queries matched: 5
gi|195337251
Mass: 102832
Score: 81
Queries matched: 5
gi|194866181
Mass: 111389
Score: 81
Queries matched: 5
gi|195125345
Mass: 111410
Score: 81
Queries matched: 5
gi|2627133
Mass: 116600
Score: 80
Queries matched: 5
gi|21429768
Mass: 119913
Score: 80
Queries matched: 5
AT20865p [Drosophila melanogaster]
Mass: 119729
Score: 80
Queries matched: 5
gi|170034793
conserved hypothetical protein [Culex quinquefasciatus]
Mass: 147245
Score: 79
Queries matched: 5
gi|62089150
ubiquitin C variant [Homo sapiens]
14.
gi|3914346
Mass: 82383
Score: 94
Queries matched: 2
emPAI: 0.04
Query
121
189
Observed
604.8083
802.4022
Mr(expt)
1207.6020
1602.7898
Mr(calc)
1207.6020
1602.7890
0.07
0
42
0.088
1
0.56
0
52 0.0067
1
gi|151555693
Mass: 82455
Score: 94
PIGR protein [Bos taurus]
15.
Peptide
K.SPIFGPEEVTSVEGR.S
Queries matched: 2
gi|7576937
Mass: 33947
Score: 94
Queries matched: 2
emPAI: 0.10
Query
100
134
Observed
571.8243
635.8719
Mr(expt)
1141.6340
1269.7292
Mr(calc)
1141.6343
1269.7293
-0.23
0
33
0.37
1
-0.01
1
60 0.00063
1
Peptide
K.VVITAPGGSDVK.T
K.KVVITAPGGSDVK.T
Mass: 35797
Score: 94
Queries matched: 2
gi|15674228
glyceraldehyde 3-phosphate dehydrogenase [Lactococcus lactis subsp. lactis Il1403]
gi|51534654
Mass: 35881
Score: 94
Queries matched: 2
glyceraldehyde-3-phosphate dehydrogenase [Streptococcus gordonii]
gi|71040530
Mass: 31074
Score: 94
Queries matched: 2
Nephritis-associated plasmin receptor [Streptococcus sp. K131]
gi|111073866
Mass: 35972
Score: 94
Queries matched: 2
glyceraldehyde-3-phosphate dehydrogenase [Streptococcus equinus]
gi|116513189
Mass: 35797
Score: 94
Queries matched: 2
glyceraldehyde 3-phosphate dehydrogenase [Lactococcus lactis subsp. cremoris SK11]
gi|157151535
Mass: 35895
Score: 94
Queries matched: 2
glyceraldehyde-3-phosphate dehydrogenase, type I [Streptococcus gordonii str. Challis substr. CH1]
12/9/2009
16.
gi|7331218
Mass: 65978
Score: 92
Queries matched: 3
Page 14 of 19
emPAI: 0.05
Query
30
69
161
Observed
437.7528
517.2615
738.3963
Mr(expt)
873.4910
1032.5084
1474.7780
Mr(calc)
873.4920
1032.5087
1474.7780
-1.06
0
22
17
2
-0.27
0
27
2
1
0.04
0
43
0.051
1
Peptide
R.SLVNLGGSK.S
R.TLLEGEESR.M
R.FLEQQNQVLQTK.W
gi|11935049
Mass: 66027
Score: 92
Queries matched: 3
gi|50979272
Mass: 63751
Score: 92
Queries matched: 3
keratin 1 [Canis lupus familiaris]
gi|109096823
Mass: 65170
Score: 92
Queries matched: 3
PREDICTED: similar to keratin 1 isoform 7 [Macaca mulatta]
gi|109096825
Mass: 64332
Score: 92
Queries matched: 3
gi|109096827
Mass: 62980
Score: 92
Queries matched: 3
Mass: 61838
Score: 92
Queries matched: 3
gi|109096829
Mass: 61566
Score: 92
Queries matched: 3
gi|109096833
gi|109096835
Mass: 58846
Score: 92
Queries matched: 3
gi|119395750
Mass: 65999
Score: 92
Queries matched: 3
gi|146741296
Mass: 65160
Score: 92
Queries matched: 3
gi|160961491
Mass: 65450
Score: 92
Queries matched: 3
Mass: 65980
Score: 92
Queries matched: 3
gi|189054178
17.
gi|6320157
Mass: 19051
Score: 85
Queries matched: 2
Query
1
126
Observed
353.7158
617.8552
Mr(expt)
705.4170
1233.6958
Mr(calc)
705.4174
1233.6969
-0.44
0
42
0.06
1
-0.84
0
42
0.03
1
Peptide
K.YVVVAR.A
K.IVEIPGEVPPGK.Y
gi|151941952
Mass: 19021
Score: 85
Queries matched: 2
gi|190405057
Mass: 19007
Score: 85
Queries matched: 2
18.
gi|83764016
Mass: 187175
Score: 82
Queries matched: 3
Query
10
68
147
Observed
400.7472
514.2980
681.3360
Mr(expt)
799.4798
1026.5814
1360.6574
Mr(calc)
799.4804
1026.5822
1360.6583
-0.64
0
23
7.7
1
-0.72
0
21
5.8
1
-0.60
1
38
0.14
1
Peptide
R.VGLVAVDK.G
R.ISLTHSLTR.V
K.SGSDEVQVKQER.K
gi|99028969
Mass: 187112
Score: 82
Queries matched: 3
gi|124056491
Mass: 187135
Score: 82
Queries matched: 3
19.
gi|123506
Mass: 8353
Score: 82
Queries matched: 3
emPAI: 1.02
Query
51
105
111
Observed
492.3031
576.7587
583.7667
Mr(expt)
982.5916
1151.5028
1165.5188
Mr(calc)
982.5923
1151.5029
1165.5186
-0.70
0
48 0.0054
1
-0.08
0
(24)
2.7
1
0.23
0
33
0.32
1
Peptide
R.ALGILNLNR.N
R.SYPSNATCPR.T + Carbamidomethyl (C)
R.SYPSNATCPR.T + Acrylamide (C)
Mass: 12474
Score: 82
Queries matched: 3
gi|5019730
gi|76782253
Mass: 12343
Score: 82
Queries matched: 3
12/9/2009
20.
gi|15673843
Mass: 43185
Score: 75
Queries matched: 2
elongation factor Tu [Lactococcus lactis subsp. lactis Il1403]
Page 15 of 19
emPAI: 0.08
Query
102
197
21.
Observed
572.8321
822.9381
Mr(expt)
1143.6496
1643.8616
Mr(calc)
1143.6499
1643.8618
-0.25
1
53 0.0044
1
-0.07
1
23
7.2
1
Peptide
R.TVGSGIVAEIKA.K.VGDEVEIVGIKEETK.K
gi|157165473
Mass: 7421
Score: 72
Queries matched: 2
sodium-dependent tyrosine transporter [Campylobacter concisus 13826]
Query
93
128
22.
Observed
563.7803
622.3538
Mr(expt)
1125.5460
1242.6930
gi|62126072
Mass: 46687
Mr(calc)
1125.5455
1242.6932
0.53
0
34
0.59
1
-0.12
1
38
0.15
1
Score: 64
Peptide
R.FYEGANQVAK.S
R.IKIDSVQDGIR.I
Queries matched: 2
Query
99
123
23.
Observed
568.3214
608.3036
Mr(expt)
1134.6282
1214.5926
Mr(calc)
1134.6285
1214.5931
gi|4927746
Mass: 23083
Score: 60
-0.19
0
34
0.33
1
-0.41
0
31
0.79
1
Queries matched: 1
Peptide
K.ETQSIIFLGK.V
R.FSISSDYQLR.D
emPAI: 0.15
Query
216
Observed
924.4029
Mr(expt)
1846.7912
Mr(calc)
1846.7897
0.83
0
60 0.0004
1
Peptide
gi|15674075
Mass: 24019
Score: 60
Queries matched: 1
gi|15674293
Mass: 24119
Score: 60
Queries matched: 1
30S ribosomal protein S3 [Streptococcus pyogenes M1 GAS]
Mass: 24031
Score: 60
Queries matched: 1
gi|15900151
30S ribosomal protein S3 [Streptococcus pneumoniae TIGR4]
gi|16801836
Mass: 24527
Score: 60
Queries matched: 1
30S ribosomal protein S3 [Listeria innocua Clip11262]
gi|24378382
Mass: 23159
Score: 60
Queries matched: 1
Mass: 24355
Score: 60
Queries matched: 1
gi|29374857
30S ribosomal protein S3 [Enterococcus faecalis V583]
gi|47093991
Mass: 23580
Score: 60
Queries matched: 1
ribosomal protein S3 [Listeria monocytogenes str. 4b H7858]
gi|47097197
Mass: 17247
Score: 60
Queries matched: 1
ribosomal protein S3 [Listeria monocytogenes str. 1/2a F6854]
Mass: 24058
Score: 60
Queries matched: 1
gi|55821899
30S ribosomal protein S3 [Streptococcus thermophilus LMG 18311]
Mass: 24333
Score: 60
Queries matched: 1
gi|69247175
ribosomal protein S3 [Enterococcus faecium DO]
gi|76798223
Mass: 23171
Score: 60
Queries matched: 1
ribosomal protein S3 [Streptococcus agalactiae 18RS21]
Mass: 24003
Score: 60
Queries matched: 1
gi|116513048
30S ribosomal protein S3 [Lactococcus lactis subsp. cremoris SK11]
gi|125716991
Mass: 24119
Score: 60
Queries matched: 1
30S ribosomal protein S3 [Streptococcus sanguinis SK36]
gi|157151263
Mass: 24050
Score: 60
Queries matched: 1
30S ribosomal protein S3 [Streptococcus gordonii str. Challis substr. CH1]
gi|161486819
Mass: 24107
Score: 60
Queries matched: 1
gi|171778382
Mass: 24161
Score: 60
Queries matched: 1
gi|195977214
Mass: 24163
Score: 60
Queries matched: 1
30S ribosomal protein S3 [Streptococcus equi subsp. zooepidemicus MGCS10565]
gi|222152271
Mass: 24134
Score: 60
Queries matched: 1
30S ribosomal protein S3 [Streptococcus uberis 0140J]
gi|228478008
Mass: 24106
Score: 60
Queries matched: 1
ribosomal protein S3 [Streptococcus salivarius SK126]
gi|229557129
Mass: 24458
Score: 60
Queries matched: 1
30S ribosomal protein S3 [Listeria grayi DSM 20601]
gi|251781521
Mass: 24147
Score: 60
Queries matched: 1
30S ribosomal protein S3 [Streptococcus dysgalactiae subsp. equisimilis GGS_124]
gi|256618374
Mass: 24311
Score: 60
Queries matched: 1
ribosomal protein S3 [Enterococcus faecalis ATCC 4200]
12/9/2009
24.
gi|28317
Mass: 59492
Score: 59
Queries matched: 1
Page 16 of 19
emPAI: 0.06
Query
150
Observed
691.3281
Mr(expt)
1380.6416
Mr(calc)
1380.6408
0.59
0
59 0.0016
1
Peptide
R.ALEESNYELEGK.I
gi|52787
Mass: 57614
Score: 59
Queries matched: 1
gi|223657
Mass: 57482
Score: 59
Queries matched: 1
keratin subunit,epidermal
gi|307086
Mass: 46359
Score: 59
Queries matched: 1
keratin-10 [Homo sapiens]
gi|387397
Mass: 57807
Score: 59
Queries matched: 1
epidermal keratin subunit I
gi|623409
Mass: 57213
Score: 59
Queries matched: 1
gi|7638398
Mass: 34962
Score: 59
Queries matched: 1
epidermal keratin 10 [Mus musculus]
gi|12852157
Mass: 58587
Score: 59
Queries matched: 1
gi|21961605
Mass: 58792
Score: 59
Queries matched: 1
gi|26349141
Mass: 52653
Score: 59
Queries matched: 1
gi|26349459
Mass: 49471
Score: 59
Queries matched: 1
Mass: 57676
Score: 59
Queries matched: 1
gi|61740600
Mass: 31541
Score: 59
Queries matched: 1
gi|87045985
keratin 10 [Oryctolagus cuniculus]
gi|109115260
Mass: 62063
Score: 59
Queries matched: 1
gi|109115262
Mass: 57547
Score: 59
Queries matched: 1
Mass: 56705
Score: 59
Queries matched: 1
gi|109115264
gi|112983636
Mass: 57007
Score: 59
Queries matched: 1
keratin complex 1, acidic, gene 10 [Mus musculus]
gi|114667507
Mass: 100282
Score: 59
Queries matched: 1
Mass: 62761
Score: 59
Queries matched: 1
gi|114667509
Mass: 58244
Score: 59
Queries matched: 1
gi|114667511
gi|114667513
Mass: 56692
Score: 59
Queries matched: 1
gi|116242600
Mass: 57735
Score: 59
Queries matched: 1
RecName: Full=Keratin, type I cytoskeletal 10; AltName: Full=Cytokeratin-10; Short=CK-10; AltName: Full=Keratin-10; Short=K10; AltName: Full=56 kDa c
gi|119581085
Mass: 63308
Score: 59
Queries matched: 1
Mass: 61580
Score: 59
Queries matched: 1
gi|149723838
PREDICTED: similar to keratin 10 [Equus caballus]
gi|194216982
Mass: 61493
Score: 59
Queries matched: 1
gi|195972866
Mass: 58766
Score: 59
Queries matched: 1
gi|251757513
Mass: 58818
Score: 59
Queries matched: 1
25.
gi|223165
Mass: 18255
lactoglobulin beta
Score: 57
Queries matched: 2
Query
39
129
26.
Observed
467.2754
623.2958
Mr(expt)
932.5362
1244.5770
Mr(calc)
932.5365
1244.5772
gi|538275
Mass: 22697
Score: 54
-0.25
0
31
0.69
1
-0.13
0
26
2
1
Queries matched: 1
Peptide
-.IIVTQTMK.G
R.TPEVDDEALEK.F
emPAI: 0.15
Query
91
Observed
561.3136
Mr(expt)
1120.6126
Mr(calc)
1120.6128
-0.16
0
54 0.0037
1
Peptide
K.DTQSIIFLGK.V
Mass: 28553
Score: 54
Queries matched: 1
gi|1245695
12/9/2009
Page 17 of 19
gi|1245697
Mass: 9788
Score: 54
Queries matched: 1
gi|31340900
Mass: 46175
Score: 54
Queries matched: 1
gi|84000377
Mass: 46297
Score: 54
Queries matched: 1
gi|121531628
Mass: 46282
Score: 54
Queries matched: 1
Mass: 46368
Score: 54
Queries matched: 1
gi|126165236
Mass: 46888
Score: 54
Queries matched: 1
gi|126165264
gi|151554250
Mass: 46935
Score: 54
Queries matched: 1
gi|160332365
Mass: 46208
Score: 54
Queries matched: 1
gi|194038345
Mass: 38368
Score: 54
Queries matched: 1
gi|194038353
Mass: 47192
Score: 54
Queries matched: 1
gi|226373737
Mass: 46208
Score: 54
Queries matched: 1
gi|226373739
Mass: 46361
Score: 54
Queries matched: 1
27.
gi|157164029
Mass: 49918
Score: 52
protease DO [Campylobacter concisus 13826]
Queries matched: 1
emPAI: 0.07
Query
76
28.
Observed
531.3135
Mr(expt)
1060.6124
Mr(calc)
1060.6128
-0.35
0
52 0.0023
1
Peptide
K.LSVVNISTTK.T
gi|157164081
Mass: 10877
Score: 51
Queries matched: 1
emPAI: 0.32
antibiotic biosynthesis monooxygenase [Campylobacter concisus 13826]
Query
71
29.
Observed
524.8054
Mr(expt)
1047.5962
Mr(calc)
1047.5964
-0.18
1
51 0.0056
1
Peptide
K.IINFVSVKE.-
gi|154174129
Mass: 12815
Score: 51
Queries matched: 1
hypothetical protein CCV52592_0624 [Campylobacter curvus 525.92]
Query
46
Observed
475.7507
Mr(expt)
949.4868
Mr(calc)
949.4869
-0.01
0
51
0.016
1
Peptide
K.ADAIIEYR.K
gi|157165213
Mass: 19144
Score: 51
Queries matched: 1
hypothetical protein CCC13826_1428 [Campylobacter concisus 13826]
Mass: 23591
Score: 51
Queries matched: 1
gi|212696196
hypothetical protein ANHYDRO_00732 [Anaerococcus hydrogenalis DSM 7454]
gi|255321667
Mass: 20963
Score: 51
Queries matched: 1
DNA uptake protein [Campylobacter showae RM3277]
Query
98
112
5
52
149
31
37
23
60
27
59
Observed
568.3210
585.3423
383.2199
492.7953
684.3511
443.7281
460.7529
428.7660
505.2633
435.7738
503.7637
Mr(expt)
1134.6274
1168.6700
764.4252
983.5760
1366.6876
885.4416
919.4912
855.5174
1008.5120
869.5330
1005.5128
Mr(calc)
1134.6284
1168.6703
764.4255
983.5764
1366.6881
885.4419
919.4916
855.5178
1008.5096
869.5334
1005.5165
ppm Miss Score
-0.88
0
44
-0.23
0
43
-0.30
0
42
-0.33
0
41
-0.32
0
40
-0.24
0
38
-0.37
0
34
-0.38
0
34
2.41
0
34
-0.46
0
30
-3.61
0
30
118
164
80
113
66
115
22
138
28
30
597.3426
745.8615
534.3138
588.8115
511.7612
596.3177
428.7660
645.8322
435.7741
437.7528
1192.6706
1489.7084
1066.6130
1175.6084
1021.5078
1190.6208
855.5174
1289.6498
869.5336
873.4910
1192.6677
1489.7121
1066.6135
1175.6121
1021.5114
1190.6216
855.5178
1289.6472
869.5334
873.4920
2.50
-2.43
-0.42
-3.11
-3.48
-0.66
-0.42
2.09
0.23
-1.06
0
1
0
0
0
1
0
1
0
0
29
29
29
29
28
28
27
26
26
25
Expect Rank
0.029
1
0.026
1
0.021
1
0.052
1
0.12
1
0.062
1
0.32
1
0.54
1
0.41
1
1.2
1
1.9
1
0.65
1.2
0.65
1.8
2.7
1.5
2.8
3.3
3.6
7.1
1
1
1
1
1
1
1
1
1
1
Peptide
AVAEYIQTLK
LIDIIEPTQK
MGGFIIK
IAGLIGVADR
ALNEINQFYQK
FSVQMFK
VFHYQVK
LALSISPR
TGMAITLMR + Oxidation (M)
VVSLSIPR
EGMAAVVSVK + Oxidation (M)
GGGHAGLGLSIVR
AETNQSQNQKQSK
DTTIHLVLR
GPYCLLQGIGR
TLTMDAVQK + Oxidation (M)
TLEEMEKALK
LATVVTPR
IMRDGMLPETK
VVSLSIPR
ISVINGGSK
12/9/2009
Page 18 of 19
209
135
237
6
140
78
119
8
120
49
179
887.9520
639.3032
809.7346
383.7209
651.8452
533.2641
599.8378
393.7395
601.3425
488.2787
783.8735
1773.8894
1276.5918
2426.1820
765.4272
1301.6758
1064.5136
1197.6610
785.4644
1200.6704
974.5428
1565.7324
1773.8937
1276.5904
2426.1826
765.4272
1301.6762
1064.5138
1197.6580
785.4647
1200.6714
974.5397
1565.7283
-2.42
1.15
-0.27
0.02
-0.24
-0.18
2.55
-0.29
-0.77
3.27
2.65
0
0
0
1
0
0
1
0
1
0
1
25
25
25
25
24
24
24
24
24
23
23
4.6
3.6
3.6
2.3
5
5.9
3.4
7
3.7
6.7
4.3
1
1
1
1
1
1
1
1
1
1
1
LFAAADLNAFVSSSFSK
VVVGEHNLNQNDGTEQYVGVQK
YILKTE
LNQIIVGCSNGK + Carbamidomethyl (C)
EDAVEFTVR
MLSGFFQKLK
NDIAIIK
VQNIIKEETK
VTAAAGTGSLK
180
43
64
12
84
89
221
74
162
47
114
210
82
72
783.8736
475.7144
510.7713
409.7238
541.2796
559.2485
964.9621
529.2731
738.8864
478.2532
595.7935
890.4479
540.2958
526.2642
1565.7326
949.4142
1019.5280
817.4330
1080.5446
1116.4824
1927.9096
1056.5316
1475.7582
954.4918
1189.5724
1778.8812
1078.5770
1050.5138
1565.7283
949.4109
1019.5321
817.4334
1080.5451
1116.4831
1927.9098
1056.5314
1475.7579
954.4957
1189.5687
1778.8799
1078.5771
1050.5128
2.78
3.47
-3.98
-0.44
-0.42
-0.61
-0.07
0.23
0.20
-4.00
3.13
0.77
-0.04
1.01
1
0
1
0
0
0
0
0
1
0
1
1
0
1
23
23
23
22
22
20
20
20
20
20
20
20
20
19
4.5
4.1
9.5
3.6
6.4
2.5
10
8
13
10
16
15
13
12
1
1
1
1
1
1
1
1
1
1
1
1
1
1
NMMAASGPR + Oxidation (M)
SIEMKVEGK
VDVNPFK
TISYENNLK
MTTFEECLK + Oxidation (M)
DDNLNPVNLLNMFHEK + Oxidation (M)
RSTAAAAAIDSSDLK
SDRQDDVSIR
QLEDGRTVTDYNIQK
HIDELSLPR
CSKGTTIER + Carbamidomethyl (C)
85
143
207
116
50
183
122
34
165
153
45
92
191
96
110
125
181
53
141
224
152
87
83
107
151
94
106
40
541.2798
442.5675
857.4084
596.8197
490.2692
529.9261
605.8375
453.7558
747.3693
713.8456
475.7415
562.8213
810.8325
565.7699
582.8106
610.7994
785.8723
493.7794
655.3228
647.6503
697.3699
556.3162
541.2670
581.8027
695.8455
563.7855
581.7905
467.7307
1080.5450
1324.6807
1712.8022
1191.6248
978.5238
1586.7565
1209.6604
905.4970
1492.7240
1425.6766
949.4684
1123.6280
1619.6504
1129.5252
1163.6066
1219.5842
1569.7300
985.5442
1308.6310
1939.9291
1392.7252
1110.6178
1080.5194
1161.5908
1389.6764
1125.5564
1161.5664
933.4468
1080.5451
1324.6848
1712.8048
1191.6248
978.5247
1586.7576
1209.6605
905.4971
1492.7270
1425.6776
949.4691
1123.6278
1619.6546
1129.5251
1163.6074
1219.5867
1569.7279
985.5444
1308.6310
1939.9318
1392.7249
1110.6145
1080.5233
1161.5931
1389.6776
1125.5567
1161.5666
933.4444
-0.07
-3.08
-1.50
0.08
-0.84
-0.72
-0.04
-0.01
-1.96
-0.67
-0.72
0.25
-2.59
0.10
-0.64
-1.97
1.35
-0.14
0.07
-1.40
0.27
2.97
-3.61
-1.93
-0.82
-0.23
-0.12
2.68
0
1
1
1
0
0
0
0
1
0
0
1
1
0
0
1
0
0
1
0
1
0
1
0
0
1
1
0
19
19
19
19
19
17
17
17
16
16
16
16
16
15
15
15
15
15
14
14
14
14
14
14
14
14
14
13
13
22
13
18
12
20
12
42
29
33
29
14
4
17
33
42
34
39
36
40
41
29
34
52
53
52
63
49
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
TISDYNIQK
LGADRSTAGGAPPR
FKCTGEKPCSVCVK + Acrylamide (C); 2 Carbamidomethyl (C)
GLIKEQYEGR
HVTLEQPR
QDENYEEAIVHLK
VIAPIDDTPAAK
NKPGVYTK
SQYEQLAEQNRK
DFTPELQASYQK
MNFQPLGK + Oxidation (M)
FVPKTTGYIV
ANEDENENEGDKEK
DQPTIEQGDK
VLDEIGVEYK
MVRIGNEEEK + Oxidation (M)
AGHMVPSDQGEMALK
IGELEAQVK
ARNDEYEIATK
IAPEHSQVMLCGNPAMVK + Oxidation (M)
TNAENEFVTIKK
LSQLPVGQNR
MSSKTSGGQAK
NLFIDTFHR
NVINEPAYTQEL
RFSGEVFER
DANEDPKFVK
SGEEWTLL
44
79
61
67
238
215
192
225
213
26
227
90
217
95
475.7285
533.3206
505.2691
512.2405
810.0621
919.4713
810.9022
972.9592
897.9113
434.7117
995.4750
559.2487
927.4694
564.2776
949.4424
1064.6266
1008.5236
1022.4664
2427.1645
1836.9280
1619.7898
1943.9038
1793.8080
867.4088
1988.9354
1116.4828
1852.9242
1126.5406
949.4440
1064.6230
1008.5240
1022.4703
2427.1596
1836.9218
1619.7937
1943.9047
1793.8036
867.4086
1988.9302
1116.4870
1852.9215
1126.5441
-1.60
3.45
-0.34
-3.73
2.00
3.43
-2.38
-0.45
2.48
0.25
2.66
-3.69
1.47
-3.05
0
1
1
0
0
1
1
1
1
0
0
0
1
0
13
13
13
13
13
12
12
12
11
11
11
11
10
10
32
9.5
49
72
61
72
72
57
85
55
77
30
1.1e+002
88
1
1
1
1
1
1
1
1
1
1
1
1
1
1
MFAPGNSAR
SLYKAGVISK
ETLASYGRL
TSMGDQIGSK
ADMPVLMAEVETAGHVLSMPAAR + 2 Oxidation (M)
DVQILLDNLKDHDGDK
EGKLMLQSQQETGR + Oxidation (M)
YGATDDYCVAGRLVQEK + Carbamidomethyl (C)
DELSEEAIMRCGTGGR + Acrylamide (C)
LGYSSDAR
YESYNLLSPNNLFCNK + Acrylamide (C)
EMGHSDSLGGK
CMHEIIFNLLKEGYK + Oxidation (M)
132
29
166
214
56
137
175
182
208
627.2769
436.7746
752.8675
608.3107
496.7977
644.8376
766.3853
794.3853
883.8950
1252.5392
871.5346
1503.7204
1821.9103
991.5808
1287.6606
1530.7560
1586.7560
1765.7754
1252.5434
871.5378
1503.7180
1821.9043
991.5815
1287.6605
1530.7579
1586.7498
1765.7802
-3.32
-3.66
1.61
3.29
-0.62
0.11
-1.22
3.97
-2.68
0
1
0
1
1
1
1
0
1
10
10
10
10
10
10
10
10
10
55
67
1e+002
1.4e+002
40
1.4e+002
1.5e+002
1.2e+002
67
1
1
1
1
1
1
1
1
1
EPDPNAFYGLM
ELKGILSL
MPNVAHFFEGVEK
LREMNNDGYLALIER + Oxidation (M)
KTGAIVGHPL
VQRTPLNTECK
IFFQTSDQAYRR
LEALESAMVSGSSYK + Oxidation (M)
WECVSGSGTKNSNQNR
12/9/2009
Page 19 of 19
228
193
177
168
186
25
133
205
229
58
219
1006.4890
813.9698
771.8700
753.8588
794.8638
432.7491
627.7685
842.9000
1042.0080
502.8139
945.9544
2010.9634
1625.9250
1541.7254
1505.7030
1587.7130
863.4836
1253.5224
1683.7854
2082.0014
1003.6132
1889.8942
2010.9568
1625.9253
1541.7297
1505.7032
1587.7174
863.4865
1253.5256
1683.7808
2082.0027
1003.6100
1889.8950
3.28
-0.16
-2.73
-0.09
-2.75
-3.30
-2.51
2.78
-0.58
3.28
-0.41
0
1
0
1
1
0
1
1
1
1
1
10
9
9
8
8
8
8
8
8
8
8
1.2e+002
40
1.1e+002
1.4e+002
81
2.5e+002
45
1.7e+002
1.8e+002
8.3
2e+002
1
1
1
1
1
1
1
1
1
1
1
SNDLGFAVSTPSGLMTVDGK + Oxidation (M)
GLISRLEISFVHQK
NFVTNFLATCNNK + Carbamidomethyl (C)
DGDVEEVCVGTGKK + Acrylamide (C)
GIPDPLPR
MKXDCLAXPVR
ETDRISVMCSELTK + Carbamidomethyl (C); Oxidation (M)
YIQCRDPETGAMPAFLVR + Oxidation (M)
MSKLITAIK
LWKGACVIMDNCSIHK + Carbamidomethyl (C); Oxidation (M)
9
24
174
32
223
198
206
222
136
169
48
178
86
194
399.1929
428.7787
766.3847
448.7179
970.9705
824.4124
843.3917
965.4523
641.8484
754.3439
480.8007
777.8376
550.2451
814.3485
796.3712
855.5428
1530.7548
895.4212
1939.9264
1646.8102
1684.7688
1928.8900
1281.6822
1506.6732
959.5868
1553.6606
1098.4756
1626.6824
796.3715
855.5430
1530.7566
895.4195
1939.9197
1646.8160
1684.7661
1928.8972
1281.6863
1506.6786
959.5838
1553.6634
1098.4764
1626.6767
-0.34
-0.12
-1.13
1.96
3.49
-3.52
1.62
-3.70
-3.19
-3.56
3.22
-1.77
-0.68
3.56
0
0
1
1
1
0
1
1
0
1
1
0
0
0
7
7
7
6
6
6
5
5
5
5
4
4
4
4
1e+002
65
2.6e+002
2.7e+002
2.8e+002
4.1e+002
2.2e+002
3.4e+002
2.7e+002
1.9e+002
62
1.4e+002
1.2e+002
1.2e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
IDYSSGR
LLVLVDGK
TKSTTWTYSESLK
RHGNCPR + Carbamidomethyl (C)
IGAYVKGTDSELDEAMNK
VCHLMGLPVSEFTK + Acrylamide (C); Oxidation (M)
THSHLCPAKSCTESK + Carbamidomethyl (C)
MIWSLSSKMVQDESTR + 2 Oxidation (M)
HMINVTLVPSR + Oxidation (M)
ECHDTKWAGHPR + Acrylamide (C)
MKQTVIIK
NWAASAEDGDVEYK
MLDGEEPHR + Oxidation (M)
METNCGTPSFSPQR + Carbamidomethyl (C); Oxidation (M)
21
176
190
226
11
97
55
13
54
220
33
19
148
2
3
7
36
42
108
130
428.2614
769.3238
807.3409
995.4746
406.2483
567.7610
496.7213
414.7617
494.3007
952.9619
450.2744
425.7607
682.2827
357.6368
362.2221
384.2351
458.7879
472.2878
581.8864
625.9131
854.5082
1536.6330
1612.6672
1988.9346
810.4820
1133.5074
991.4280
827.5088
986.5868
1903.9092
898.5342
849.5068
1362.5508
713.2590
722.4296
766.4556
915.5612
942.5610
1161.7582
1249.8116
854.5086
1536.6328
1612.6684
1988.9410
810.4824
1133.5063
991.4289
827.5116
986.5873
1903.9033
898.5348
849.5072
1362.5535
-0.44
0.14
-0.70
-3.18
-0.46
1.02
-0.87
-3.38
-0.41
3.15
-0.65
-0.45
-1.93
1
0
0
1
1
0
0
0
1
1
1
0
0
4
3
3
3
3
3
3
2
2
1
1
0
0
1.8e+002
95
1.3e+002
4.7e+002
1e+002
2.5e+002
1.9e+002
4.1e+002
3.1e+002
1e+003
1.1e+003
5.5e+002
1.5e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
RVPALSGR
EDAGSWVSGESEER
NCDPNGPLMCYVSK + Carbamidomethyl (C); Oxidation (M)
LITQFMEPMGFVKCEK + Carbamidomethyl (C); 2 Oxidation (M)
GPVVARGR
DAYMISQYK + Oxidation (M)
MVMHMAEK + Oxidation (M)
ILVPSATK
LKAAGATLSR
LCSPRQSEAECWGVALR
VVALGERR
IVTAPPPR
AAEAEAATENSEGN
Search Parameters
Type of search
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
MS/MS Ion Search
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
238
12/9/2009
Page 1 of 8
User
Email
Search title
MS data file
Database
Timestamp
Protein hits
:
:
:
:
:
:
:
LTQFT Daemon
D:\Data\Mark\18_7-09\N_18_7_09_3.RAW
9 Dec 2009 at 03:57:40 GMT
gi|122411
gi|1942778
Chain A, Deoxy-Haemoglobin Trapped In The High-Affinity (R) State
gi|122614
RecName: Full=Hemoglobin subunit beta; AltName: Full=Hemoglobin beta chain; AltName: Full=Beta-globin
gi|2136760
gi|157164064 ModE family transcriptional regulator [Campylobacter concisus 13826]
gi|2266432
gi|3914346
gi|123506
gi|136429
gi|157164945 DNA-binding protein HU 1 (DNA-binding protein II; HB) [Campylobacter concisus 13826]
gi|7576937
gi|229552
albumin
gi|27806789 transthyretin [Bos taurus]
gi|6320157
gi|494583
Chain A, Structure Solution And Molecular Dynamics Refinement Of The Yeast Cu,Zn Enzyme Superoxide Dismutase
gi|57164373 pre-pro serum albumin [Ovis aries]
gi|157164111 hypothetical protein CCC13826_1707 [Campylobacter concisus 13826]
gi|158604931 histidine triad nucleotide-binding protein 1 (Adenosine5'-monophosphoramidase) (P13.7) [Campylobacter concisus 13826]
gi|11467637 Ycf35 [Guillardia theta]
gi|538275
25
1
4.00 %
43
1
2.33 %
Format As
Standard scoring
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1.
Help
Peptide Summary
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MudPIT scoring
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Decreasing Score
Error tolerant
Require bold red
Archive Report
gi|158604950
Mass: 13498
Score: 345
Queries matched: 10
emPAI: 0.96
Query
54
60
Observed
505.2636
511.2690
Mr(expt)
1008.5126
1020.5234
Mr(calc)
1008.5128
1020.5240
ppm Miss Score
-0.11
0
25
-0.54
0
49
80
558.3008
1114.5870
1114.5870
0.05
0
91
110
125
137
164
165
166
575.3165
622.3484
660.8350
738.8862
794.4042
794.4043
529.9388
1148.6184
1242.6822
1319.6554
1475.7578
1586.7938
1586.7940
1586.7946
1148.6190
1242.6819
1319.6568
1475.7579
1586.7940
1586.7940
1586.7940
-0.44
0.24
-1.06
-0.07
-0.11
0.02
0.35
1
1
0
1
0
0
0
29
Expect Rank
2.8
2
0.035
1
Peptide
K.SAFLDELSK.A
K.YDQIVDIR.T
1.1
1
K.TVNISPDEIK.K
40
0.13
32
0.66
70 0.00015
43
0.063
(30)
1.3
56
0.0031
(31)
0.95
1
1
1
1
1
1
1
K.KYDQIVDIR.T
K.TVNISPDEIKK.Y
R.STAAAAAIDSSDLK.I
R.RSTAAAAAIDSSDLK.I
R.TPSEWQETGIIAGAK.T
R.TPSEWQETGIIAGAK.T
R.TPSEWQETGIIAGAK.T
12/9/2009
2.
Page 2 of 8
gi|122411
Mass: 15236
Score: 271
Queries matched: 13
emPAI: 1.24
Query
11
12
65
67
Observed
409.7237
409.7239
521.2753
529.2729
Mr(expt)
817.4328
817.4332
1040.5360
1056.5312
Mr(calc)
817.4334
817.4334
1040.5365
1056.5314
ppm Miss Score
-0.69
0
26
-0.20
0
(20)
-0.43
0
(36)
-0.16
0
45
74
75
89
90
115
147
148
149
150
544.3165
544.3165
573.3296
573.3297
634.8584
505.9107
758.3629
505.9111
758.3631
1086.6184
1086.6184
1144.6446
1144.6448
1267.7022
1514.7103
1514.7112
1514.7115
1514.7116
1086.6186
1086.6186
1144.6452
1144.6452
1267.7024
1514.7113
1514.7113
1514.7113
1514.7113
-0.12
-0.12
-0.46
-0.28
-0.10
-0.71
-0.07
0.08
0.20
1
1
1
1
0
0
0
0
0
Expect Rank
1.5
1
5.2
3
0.2
1
0.033
1
34
0.49
(25)
4.7
63 0.00042
(57) 0.0018
46
0.016
(39)
0.1
57
0.0014
(44)
0.033
(49) 0.0097
Peptide
R.VDPVNFK.L
R.VDPVNFK.L
R.MFLGFPTTK.T
1
1
1
1
1
4
1
2
1
K.LRVDPVNFK.L
K.LRVDPVNFK.L
M.VLSAADKTNVK.A
M.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
gi|230479
Mass: 15105
Score: 271
Queries matched: 13
Mass: 15105
Score: 271
Queries matched: 13
gi|230632
gi|313500
Mass: 15236
Score: 271
Queries matched: 13
Mass: 15105
Score: 271
Queries matched: 13
gi|24158717
3.
gi|1942778
Mass: 15089
Score: 260
Queries matched: 11
emPAI: 1.25
Query
11
12
65
67
74
75
89
90
115
142
143
Observed
409.7237
409.7239
521.2753
529.2729
544.3165
544.3165
573.3296
573.3297
634.8584
500.5793
750.3654
Mr(expt)
817.4328
817.4332
1040.5360
1056.5312
1086.6184
1086.6184
1144.6446
1144.6448
1267.7022
1498.7161
1498.7162
Proteins matching the
Mass:
gi|146149083
alpha 2 globin [Equus
gi|146149272
Mass:
4.
Mr(calc)
817.4334
817.4334
1040.5365
1056.5314
1086.6186
1086.6186
1144.6452
1144.6452
1267.7024
1498.7164
1498.7164
-0.69
0
26
1.5
1
-0.20
0
(20)
5.2
3
-0.43
0
(36)
0.2
1
-0.16
0
45
0.033
1
-0.12
1
34
0.49
1
-0.12
1
(25)
4.7
1
-0.46
1
63 0.00042
1
-0.28
1
(57) 0.0018
1
-0.10
0
46
0.016
1
-0.25
0
(43)
0.05
1
-0.13
0
46
0.021
1
same set of peptides:
15220
Score: 260
caballus]
15220
Score: 260
caballus]
Peptide
R.VDPVNFK.L
R.VDPVNFK.L
R.MFLGFPTTK.T
K.LRVDPVNFK.L
K.LRVDPVNFK.L
-.VLSAADKTNVK.A
-.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEFGAEALER.M
K.VGGHAGEFGAEALER.M
Queries matched: 11
Queries matched: 11
gi|122614
Mass: 15998
Score: 253
Queries matched: 9
emPAI: 1.62
Query
83
93
117
132
134
136
196
197
198
Observed
563.7856
575.3405
637.8661
713.8458
483.9367
725.4017
1000.4640
672.6435
1008.4620
Mr(expt)
1125.5566
1148.6664
1273.7176
1425.6770
1448.7883
1448.7888
1998.9134
2014.9087
2014.9094
Mr(calc)
1125.5567
1148.6666
1273.7183
1425.6776
1448.7888
1448.7888
1998.9146
2014.9095
2014.9095
ppm Miss Score
Expect Rank
-0.05
0
21
10
1
-0.11
0
51
0.0025
1
-0.51
0
44
0.03
1
-0.39
0
32
0.68
1
-0.38
1
(29)
0.89
1
0.02
1
29
0.88
1
-0.57
0
(52)
0.0048
1
-0.41
0
(55)
0.0024
1
-0.03
0
76 1.9e-005
1
Peptide
K.LHVDPENFR.L
K.VVAGVANALAHK.Y
R.LLVVYPWTQR.F
K.DFTPELQASYQK.V
K.VVAGVANALAHKYH.K.VVAGVANALAHKYH.R.FFDSFGDLSNPGAVMGNPK.V
R.FFDSFGDLSNPGAVMGNPK.V + Oxidation (M)
gi|230480
Mass: 15970
Score: 253
Queries matched: 9
Chain B, Three Dimensional Fourier Synthesis Of Horse Deoxyhaemoglobin At 2.8 Angstroms Resolution
gi|255683515
Mass: 16129
Score: 253
Queries matched: 9
hemoglobin, beta [Equus caballus]
5.
gi|2136760
Mass: 44735
Score: 162
Queries matched: 4
emPAI: 0.07
12/9/2009
Query
15
57
104
133
Observed
419.7186
508.2980
597.3248
720.3572
Mr(expt)
837.4226
1014.5814
1192.6350
1438.6998
Mr(calc)
837.4232
1014.5822
1192.6353
1438.6993
-0.66
0
28
2.3
1
-0.74
0
48
0.018
1
-0.21
0
50
0.012
1
0.35
0
36
0.26
1
Page 3 of 8
Peptide
R.AGSAEYLK.T
R.VTGIITQGAR.D
R.QFQFIQVAGR.S
R.DFGHIQYVAAYR.V
gi|2494285
Mass: 47380
Score: 162
Queries matched: 4
gi|2652932
Mass: 45502
Score: 162
Queries matched: 4
gi|41386719
Mass: 47471
Score: 162
Queries matched: 4
Mass: 47831
Score: 162
Queries matched: 4
gi|74354284
6.
gi|157164064
Mass: 14431
Score: 154
Queries matched: 3
emPAI: 0.24
ModE family transcriptional regulator [Campylobacter concisus 13826]
Query
36
64
123
7.
Observed
464.7947
515.7982
650.8462
Mr(expt)
927.5748
1029.5818
1299.6778
gi|2266432
Mass: 59208
Mr(calc)
927.5753
1029.5818
1299.6783
-0.51
0
44
0.039
1
0.00
0
63 0.00065
1
-0.31
0
47
0.022
1
Score: 151
Queries matched: 7
Peptide
K.ATQIIVGVK.R.TGAVNSLIAGK.L
R.NQLNVEISEVR.T
emPAI: 0.18
Query
126
127
128
162
173
174
175
Observed
442.5674
663.3480
671.3452
529.9263
840.9492
560.9686
840.9493
Mr(expt)
1324.6804
1324.6814
1340.6758
1586.7571
1679.8838
1679.8840
1679.8840
Mr(calc)
1324.6809
1324.6809
1340.6758
1586.7576
1679.8842
1679.8842
1679.8842
-0.40
0
(35)
0.57
1
0.41
0
55
0.0063
1
0.02
0
(44)
0.037
1
-0.34
0
34
0.45
1
-0.24
0
(37)
0.18
1
-0.16
0
(30)
0.79
1
-0.12
0
62 0.00048
1
Peptide
K.VAALGSDPHISMK.V
K.VAALGSDPHISMK.V
K.VAALGSDPHISMK.V + Oxidation (M)
R.QDENYEEAIVHLK.V
Mass: 59239
Score: 151
Queries matched: 7
gi|3183510
gi|12647311
Mass: 32265
Score: 151
Queries matched: 7
gi|27806233
Mass: 59225
Score: 151
Queries matched: 7
8.
gi|3914346
Mass: 82383
Score: 123
Queries matched: 5
Query
46
102
103
106
118
9.
Observed
488.2790
398.2150
596.8194
604.8080
640.8819
Mr(expt)
974.5434
1191.6232
1191.6242
1207.6014
1279.7492
Mr(calc)
974.5437
1191.6248
1191.6248
1207.6020
1279.7500
-0.23
0
27
3.6
1
-1.33
1
(22)
5.9
1
-0.43
1
40
0.14
1
-0.42
0
24
5.8
2
-0.58
1
32
0.12
1
Peptide
R.WISTVELK.V
R.GLIKEQYEGR.L
R.GLIKEQYEGR.L
K.VVQGEPSLKVPK.N
gi|123506
Mass: 8353
Score: 122
Queries matched: 2
emPAI: 1.02
Query
47
121
Observed
492.3034
644.3453
Mr(expt)
982.5922
1286.6760
Mr(calc)
982.5923
1286.6765
ppm Miss Score
Expect Rank
-0.08
0
48
0.0052
1
-0.34
0
74 5.6e-005
1
Peptide
R.ALGILNLNR.N
R.NLQLILNSCGR.S + Carbamidomethyl (C)
gi|5019730
Mass: 12474
Score: 122
Queries matched: 2
gi|76782253
Mass: 12343
Score: 122
Queries matched: 2
10.
gi|136429
Mass: 24394
Score: 122
Queries matched: 6
emPAI: 0.29
Query
Observed
Mr(expt)
Mr(calc)
ppm
Miss Score Expect Rank
Peptide
12/9/2009
17
18
19
66
199
200
421.7582
421.7582
421.7583
523.2852
737.7056
737.7060
841.5018
841.5018
841.5020
1044.5558
2210.0950
2210.0962
841.5022
841.5022
841.5022
1044.5564
2210.0967
2210.0967
-0.36
-0.36
-0.12
-0.48
-0.79
-0.25
0
0
0
0
0
0
(37)
0.31
56 0.0042
(40)
0.17
27
2.5
(26)
2.8
39
0.14
1
1
1
1
1
1
Page 4 of 8
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
K.LSSPATLNSR.V
Mass: 23458
Score: 122
Queries matched: 6
gi|494360
Chain A, The Refined 1.6 Angstroms Resolution Crystal Structure Of The Complex Formed Between Porcine Beta-Trypsin And Mcti-A, A Trypsin Inhibitor Of
gi|999627
Mass: 8814
Score: 122
Queries matched: 6
Chain B, Refined 1.8 Angstroms Resolution Crystal Structure Of Porcine Epsilon-Trypsin
gi|1942351
Mass: 13284
Score: 122
Queries matched: 6
Chain A, Crystal Structure Of The First Active Autolysate Form Of The Porcine Alpha Trypsin
gi|2914482
Mass: 23460
Score: 122
Queries matched: 6
gi|3318722
Mass: 23457
Score: 122
Queries matched: 6
gi|110590762
Mass: 23458
Score: 122
Queries matched: 6
gi|157878102
Mass: 24157
Score: 122
Queries matched: 6
Chain E, Complex Of Eeti-Ii With Porcine Trypsin
gi|242253868
Mass: 25865
Score: 122
Queries matched: 6
11.
gi|157164945
Mass: 10510
Score: 111
Queries matched: 2
emPAI: 0.76
DNA-binding protein HU 1 (DNA-binding protein II; HB) [Campylobacter concisus 13826]
Query
63
76
12.
Observed
514.8085
546.2900
Mr(expt)
1027.6024
1090.5654
Mr(calc)
1027.6026
1090.5659
-0.10
1
58 0.0021
1
-0.38
0
53 0.0083
1
Peptide
K.LKEAVAAGAAK.K
K.AEFIQAVADK.A
gi|7576937
Mass: 33947
Score: 103
Queries matched: 2
emPAI: 0.10
Query
86
116
Observed
571.8241
635.8721
Mr(expt)
1141.6336
1269.7296
Mr(calc)
1141.6343
1269.7293
ppm Miss Score
Expect Rank
-0.58
0
32
0.44
1
0.30
1
71 5.8e-005
1
Peptide
K.VVITAPGGSDVK.T
K.KVVITAPGGSDVK.T
gi|15674228
Mass: 35797
Score: 103
Queries matched: 2
glyceraldehyde 3-phosphate dehydrogenase [Lactococcus lactis subsp. lactis Il1403]
gi|51534654
Mass: 35881
Score: 103
Queries matched: 2
glyceraldehyde-3-phosphate dehydrogenase [Streptococcus gordonii]
gi|71040530
Mass: 31074
Score: 103
Queries matched: 2
Nephritis-associated plasmin receptor [Streptococcus sp. K131]
gi|111073866
Mass: 35972
Score: 103
Queries matched: 2
glyceraldehyde-3-phosphate dehydrogenase [Streptococcus equinus]
gi|116513189
Mass: 35797
Score: 103
Queries matched: 2
glyceraldehyde 3-phosphate dehydrogenase [Lactococcus lactis subsp. cremoris SK11]
gi|157151535
Mass: 35895
Score: 103
Queries matched: 2
glyceraldehyde-3-phosphate dehydrogenase, type I [Streptococcus gordonii str. Challis substr. CH1]
13.
gi|229552
albumin
Mass: 66088
Score: 97
Queries matched: 3
Query
52
Observed
501.7951
Mr(expt)
1001.5756
Mr(calc)
1001.5757
-0.07
0
21
7
1
Peptide
K.LVVSTQTALA.-
56
96
507.8132
582.3187
1013.6118
1162.6228
1013.6121
1162.6234
-0.22
-0.45
K.QTALVELLK.H
K.LVNELTEFAK.T
0
0
40
37
0.057
0.23
1
1
Mass: 69248
Score: 97
Queries matched: 3
gi|1351907
gi|30794280
Mass: 69278
Score: 97
Queries matched: 3
gi|74267962
Mass: 69190
Score: 97
Queries matched: 3
gi|154425704
Mass: 69248
Score: 97
Queries matched: 3
14.
gi|27806789
Mass: 15717
transthyretin [Bos taurus]
Score: 96
Queries matched: 2
emPAI: 0.48
12/9/2009
Query
31
131
Observed
450.2508
704.8227
Mr(expt)
898.4870
1407.6308
Mr(calc)
898.4872
1407.6306
-0.20
0
48
0.014
1
0.14
0
49 0.0083
1
Page 5 of 8
Peptide
R.GSPAANVGVK.V
K.AADETWEPFASGK.T
gi|57526651
Mass: 15761
Score: 96
Queries matched: 2
transthyretin [Ovis aries]
15.
gi|6320157
Mass: 19051
Score: 76
Queries matched: 2
emPAI: 0.18
Query
1
108
Observed
353.7158
617.8555
Mr(expt)
705.4170
1233.6964
Mr(calc)
705.4174
1233.6969
-0.44
0
26
2.9
4
-0.35
0
50 0.0049
1
Peptide
K.YVVVAR.A
K.IVEIPGEVPPGK.Y
Mass: 19021
Score: 76
Queries matched: 2
gi|151941952
gi|190405057
Mass: 19007
Score: 76
Queries matched: 2
16.
gi|494583
Mass: 15714
Score: 66
Queries matched: 2
Query
50
71
Observed
499.8032
541.7646
Mr(expt)
997.5918
1081.5146
Mr(calc)
997.5920
1081.5152
-0.19
0
45
0.023
1
-0.55
0
21
6.7
1
Peptide
K.LIGPTSVVGR.S
K.THGAPTDEVR.H
gi|6322564
Mass: 15845
Score: 66
Queries matched: 2
Cytosolic superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; Sod1p [Saccharomyces
gi|6730103
Mass: 15680
Score: 66
Queries matched: 2
Chain A, H48c Yeast Cu(Ii)ZN SUPEROXIDE DISMUTASE ROOM TEMPERATURE (298k) Structure
Mass: 15724
Score: 66
Queries matched: 2
gi|15826571
Chain A, Heterodimer Between H48f-Ysod1 And Yccs
Mass: 15944
Score: 66
Queries matched: 2
gi|27573538
Chain A, Crystal Structure Of Yeast Copper-Zinc Superoxide Dismutase Mutant Gly85arg
gi|27573539
Mass: 15836
Score: 66
Queries matched: 2
Chain A, Crystal Structure Of Yeast H48q Cuznsod Fals Mutant Analog
gi|27573540
Mass: 15811
Score: 66
Queries matched: 2
Chain A, Crystal Structure Of Yeast H46c Cuznsod Mutant
gi|31321998
Mass: 14190
Score: 66
Queries matched: 2
cytosolic copper-zinc superoxide dismutase isoform 2 [Debaryomyces hansenii]
17.
gi|57164373
Mass: 69143
Score: 64
pre-pro serum albumin [Ovis aries]
Queries matched: 2
Query
7
56
Observed
395.2392
507.8132
Mr(expt)
788.4638
1013.6118
Mr(calc)
788.4644
1013.6121
-0.66
0
25
2.3
1
-0.22
0
40
0.057
1
gi|193085052
Mass: 66270
Score: 64
albumin precursor [Capra hircus]
18.
Peptide
K.IVTDLTK.V
K.QTALVELLK.H
Queries matched: 2
gi|157164111
Mass: 16254
Score: 59
Queries matched: 1
emPAI: 0.21
Query
144
19.
Observed
751.3617
Mr(expt)
1500.7088
Mr(calc)
1500.7096
-0.53
0
59 0.0015
1
Peptide
K.DGSQEVFATYIGSK.K
gi|158604931
Mass: 13066
Score: 57
Queries matched: 2
emPAI: 0.26
histidine triad nucleotide-binding protein 1 (Adenosine5'-monophosphoramidase) (P13.7) [Campylobacter concisus 13826]
Query
100
140
20.
Observed
589.8133
747.3481
Mr(expt)
1177.6120
1492.6816
Mr(calc)
1177.6131
1492.6834
-0.93
0
25
4.7
1
-1.20
0
32
0.54
1
Peptide
K.FLAFNDINPK.A
K.LGWSEGVADPQSTF.-
gi|11467637
Mass: 15089
Score: 57
Queries matched: 1
emPAI: 0.23
Ycf35 [Guillardia theta]
12/9/2009
Query
51
21.
Observed
501.2849
Mr(expt)
1000.5552
Mr(calc)
1000.5553
gi|538275
Mass: 22697
Score: 54
-0.03
0
57 0.0036
1
Queries matched: 1
Page 6 of 8
Peptide
K.QSLNDLAIK.W
emPAI: 0.15
Query
81
Observed
561.3135
Mr(expt)
1120.6124
Mr(calc)
1120.6128
-0.33
0
54 0.0038
1
Peptide
K.DTQSIIFLGK.V
gi|1245695
Mass: 28553
Score: 54
Queries matched: 1
gi|1245697
Mass: 9788
Score: 54
Queries matched: 1
gi|31340900
Mass: 46175
Score: 54
Queries matched: 1
gi|84000377
Mass: 46297
Score: 54
Queries matched: 1
gi|121531628
Mass: 46282
Score: 54
Queries matched: 1
gi|126165236
Mass: 46368
Score: 54
Queries matched: 1
gi|126165264
Mass: 46888
Score: 54
Queries matched: 1
Mass: 46935
Score: 54
Queries matched: 1
gi|151554250
Mass: 46208
Score: 54
Queries matched: 1
gi|160332365
gi|194038345
Mass: 38368
Score: 54
Queries matched: 1
gi|194038353
Mass: 47192
Score: 54
Queries matched: 1
Mass: 46208
Score: 54
Queries matched: 1
gi|226373737
gi|226373739
Mass: 46361
Score: 54
Queries matched: 1
Query
78
111
187
139
147
58
188
149
23
138
59
172
10
87
72
98
29
171
114
130
159
54
1
101
37
61
12
6
27
106
68
20
85
69
177
Observed
550.2995
623.2957
914.4415
745.3687
505.9107
508.7720
914.4421
505.9111
428.7660
739.8376
510.7713
827.4566
407.7370
572.8322
543.2922
588.8114
443.7280
551.9733
629.8124
681.3369
783.8737
505.2636
353.7158
596.3183
471.7843
511.7613
409.7239
393.7396
435.7738
604.8080
533.2639
424.7529
565.7698
533.2640
565.9646
Mr(expt)
1098.5844
1244.5768
1826.8684
1488.7228
1514.7103
1015.5294
1826.8696
1514.7115
855.5174
1477.6606
1019.5280
1652.8986
813.4594
1143.6498
1084.5698
1175.6082
885.4414
1652.8981
1257.6102
1360.6592
1565.7328
1008.5126
705.4170
1190.6220
941.5540
1021.5080
817.4332
785.4646
869.5330
1207.6014
1064.5132
847.4912
1129.5250
1064.5134
1694.8720
Mr(calc)
1098.5856
1244.5772
1826.8686
1488.7242
1514.7113
1015.5298
1826.8686
1514.7113
855.5178
1477.6606
1019.5321
1652.8985
813.4596
1143.6499
1084.5699
1175.6047
885.4419
1652.8985
1257.6088
1360.6583
1565.7283
1008.5096
705.4174
1190.6217
941.5546
1021.5114
817.4334
785.4647
869.5334
1207.5986
1064.5138
847.4916
1129.5251
1064.5138
1694.8661
ppm Miss Score
-1.02
0
49
-0.29
0
47
-0.10
0
46
-0.93
0
45
-0.71
0
45
-0.33
0
44
0.55
0
44
0.08
0
44
-0.40
0
43
0.04
0
43
-3.99
0
42
0.08
0
42
-0.22
0
42
-0.07
1
41
-0.08
0
36
3.01
1
36
-0.47
0
35
-0.27
0
34
1.12
0
33
0.72
1
32
2.90
1
31
3.01
0
30
-0.44
0
30
0.33
0
29
-0.57
0
27
-3.28
1
26
-0.20
0
26
-0.05
0
25
-0.46
0
25
2.38
0
24
-0.54
0
24
-0.39
0
24
-0.07
0
23
-0.35
0
23
3.44
1
22
Expect Rank
0.011
1
0.011
1
0.027
1
0.039
1
0.032
1
0.057
1
0.043
1
0.033
1
0.061
1
0.031
1
0.11
1
0.035
1
0.066
1
0.071
1
0.24
1
0.35
1
0.14
1
0.27
1
0.5
1
0.63
1
0.64
1
0.92
1
1.1
1
1.2
1
1.9
1
3.9
1
1.6
1
4.5
1
3.9
1
5
1
5.6
1
4.8
1
2.8
1
7.5
1
6.3
1
Peptide
LVGVHSSVCK + Acrylamide (C)
TPEVDDEALEK
GEADALNLDGGYIYTAGK
NGELVEQMVGAQSK
VGAHAGDYGAEALER
GESIIEAAAR
GEADALNLDGGYIYTAGK
VGGHAADYGAEALER
IAVTLSPR
IMENQSEELEEK
MNSVTELVK
VGAPLVEGATVVGEVEK
VVDVPASK
TVGSGIVAEIKA
LVGVHSSVCK + Carbamidomethyl (C)
TSNSWAKVQR
FSVQMFK
VGAPLVEGATVVGEVEK
LPEGVSLENTEA
SGSDEVQVKQER
TGMAITLMR + Oxidation (M)
YVVGIR
QVVDAVALK
DVTDMLKGK + Oxidation (M)
VDVNPFK
LALAVGDK
VVSLSIPR
ISTLDFWGNR
EDADIFISR
KPTFSLR
DQPTIEQGDK
EDADIFISR
SFNLAVSMLEQQKGK + Oxidation (M)
12/9/2009
Page 7 of 8
42
26
5
129
191
45
43
179
107
73
88
478.2534
433.7600
393.7394
674.8696
632.6476
482.2532
478.7737
857.4074
608.3507
363.2131
382.5554
954.4922
865.5054
785.4642
1347.7246
1894.9210
962.4918
955.5328
1712.8002
1214.6868
1086.6175
1144.6444
954.4957
865.5062
785.4647
1347.7245
1894.9214
962.4920
955.5338
1712.7941
1214.6870
1086.6186
1144.6452
-3.58
-0.83
-0.54
0.07
-0.20
-0.19
-1.03
3.62
-0.15
-1.02
-0.70
0
0
0
1
1
0
0
1
1
1
1
22
22
21
20
20
20
20
20
20
19
19
4.3
3.6
12
9.2
13
11
7.9
11
8.6
10
9.8
1
1
1
1
1
1
1
1
1
1
1
AQLFLFK
NDIAIIK
ASSVIVSKDDSIK
HFGKDFTPELQASYQK
ATITVDSEK
TPAGVAEAIK
AFGPGPSNGYTAGSRMK + Oxidation (M)
QAITDKLNSLL
LRVDPVNFK
VLSAADKNTVK
158
161
97
33
119
135
25
105
62
38
40
183
141
152
783.8733
785.8728
584.3209
453.7555
427.5905
483.9368
432.7488
600.2894
512.2404
472.1944
475.7144
589.6295
747.8350
763.8796
1565.7320
1569.7310
1166.6272
905.4964
1279.7497
1448.7886
863.4830
1198.5642
1022.4662
942.3742
949.4142
1765.8667
1493.6554
1525.7446
1565.7283
1569.7344
1166.6295
905.4970
1279.7540
1448.7888
863.4865
1198.5652
1022.4703
942.3753
949.4175
1765.8669
1493.6555
1525.7446
2.39
-2.16
-1.94
-0.65
-3.39
-0.17
-3.98
-0.81
-3.92
-1.09
-3.40
-0.11
-0.04
0.01
1
1
1
1
1
1
1
1
0
0
0
0
0
0
19
19
18
18
18
17
17
17
17
16
16
16
16
15
13
12
11
27
3.7
13
38
16
30
3.4
20
32
16
30
1
1
1
1
1
1
1
1
1
1
1
1
1
1
VIKDEGIEHK
KNFEQLK
VVKADFLTFLK
VVAGVANALAHKYH
VAEKVYR
DTYTKCANVK + Carbamidomethyl (C)
TSMGDQIGSK
MQDNYEK + Oxidation (M)
EAQNDMVK + Oxidation (M)
DTKPGTNNSAIFMNIK + Oxidation (M)
IMENQSEELEEK + Oxidation (M)
SSGSSYPSLLQCLK + Carbamidomethyl (C)
181
41
16
92
70
94
13
124
82
145
146
30
55
176
180
184
49
79
178
153
84
160
34
186
185
28
154
163
875.9434
475.7287
419.7188
383.8960
534.2760
581.7908
414.2711
655.3224
563.7855
752.8669
753.3467
448.7179
505.2691
842.8991
584.2977
883.9427
496.7979
556.3167
848.4445
765.3523
564.2775
785.3224
457.7687
897.9116
887.9530
436.7746
769.3242
794.3881
1749.8722
949.4428
837.4230
1148.6662
1066.5374
1161.5670
826.5276
1308.6302
1125.5564
1503.7192
1504.6788
895.4212
1008.5236
1683.7836
1749.8713
1765.8708
991.5812
1110.6188
1694.8744
1528.6900
1126.5404
1568.6302
913.5228
1793.8086
1773.8914
871.5346
1536.6338
1586.7616
1749.8719
949.4426
837.4232
1148.6627
1066.5342
1161.5634
826.5276
1308.6350
1125.5601
1503.7214
1504.6729
895.4195
1008.5240
1683.7886
1749.8735
1765.8669
991.5815
1110.6219
1694.8774
1528.6866
1126.5441
1568.6339
913.5246
1793.8050
1773.8857
871.5378
1536.6288
1586.7576
0.17
0.22
-0.19
2.99
3.05
3.13
0.02
-3.64
-3.21
-1.41
3.96
1.96
-0.34
-2.95
-1.26
2.25
-0.22
-2.78
-1.72
2.27
-3.23
-2.34
-1.91
2.05
3.24
-3.66
3.29
2.54
0
0
0
1
1
0
0
0
0
0
1
1
1
1
1
0
1
0
1
1
0
0
1
1
1
1
0
0
15
15
15
14
14
14
14
13
13
13
12
12
12
12
11
11
11
10
10
10
10
10
10
10
10
9
9
9
42
25
44
13
54
70
29
45
64
70
50
74
62
87
1e+002
98
34
67
74
61
97
15
1.3e+002
1.3e+002
1.5e+002
78
20
1.1e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
DTKPGTNNSAIFMNIK
NDLTLDML + Oxidation (M)
NTELAYK
KFLSCLLTPK
MNTFRVQR + Oxidation (M)
MIAEACAAVAR + Carbamidomethyl (C)
VQAVAVLK
FNGLEDVPQYK
LLECLGHGER
NIPVLMEHYMNK + Oxidation (M)
CSDYVDPTHKGGR + Acrylamide (C)
NGRHCPR + Carbamidomethyl (C)
ETLASYGRL
VFDNTGKQMASSAANK + Oxidation (M)
FFALCLDAFRAMFK + Acrylamide (C)
TKGFAITVR
VVALAMAPPSR
FRTMLIQAISEGSSR
REQDQQQEPSER
DDSSGYGSASTHTER
ARLYVHR
SGVYAACHATSGACRGR + Acrylamide (C); Carbamidomethyl (C)
RAVVEEVSGVTEQESR
ELKGILSL
NSDVSSANENGSDNK
NGNEPPEEAFLATAK
182
155
167
169
95
190
48
192
8
193
195
189
122
156
883.8956
769.3545
796.3138
810.8329
581.8031
927.4689
494.3007
964.9622
399.1930
970.9705
995.4760
921.9124
649.2776
774.3577
1765.7766
1536.6944
1590.6130
1619.6512
1161.5916
1852.9232
986.5868
1927.9098
796.3714
1939.9264
1988.9374
1841.8102
1296.5406
1546.7008
1765.7802
1536.6925
1590.6166
1619.6546
1161.5924
1852.9215
986.5873
1927.9132
796.3715
1939.9237
1988.9370
1841.8076
1296.5373
1546.7007
-2.00
1.24
-2.25
-2.10
-0.67
0.93
-0.42
-1.72
-0.09
1.41
0.24
1.41
2.56
0.10
1
1
0
1
1
0
1
1
0
0
1
0
1
1
9
9
8
8
8
8
8
8
7
7
6
5
5
5
71
99
11
21
2e+002
2.1e+002
82
1.8e+002
90
2.2e+002
2.7e+002
1.6e+002
92
2e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
WECVSGSGTKNSNQNR
NNQMTSCEIWRR
QMVLDXTFCPSXR + Carbamidomethyl (C); Oxidation (M)
ANEDENENEGDKEK
TQCRDSILR + Acrylamide (C)
KPITFENIPNCYICK + Acrylamide (C)
VVKNTLASR
CQDMLLRGNSDYSLLGK + Oxidation (M)
ISDYGSR
AFDNCILTIEAYDPDIK
ASVVCMMAGADFIKTSTGK + Carbamidomethyl (C); Oxidation (M)
TLSMFEWTASHCSGAK + Acrylamide (C); Oxidation (M)
MCGPDGMSRVR + Carbamidomethyl (C); 2 Oxidation (M)
YSSSKSCMEELVK + Carbamidomethyl (C)
24
77
120
113
151
194
22
53
109
428.7787
550.2451
641.8486
627.2769
761.3562
972.9586
428.2616
502.8141
622.3171
855.5428
1098.4756
1281.6826
1252.5392
1520.6978
1943.9026
854.5086
1003.6136
1242.6196
855.5429
1098.4764
1281.6870
1252.5436
1520.6963
1943.8968
854.5086
1003.6100
1242.6213
-0.11
-0.69
-3.37
-3.51
1.04
2.99
0.03
3.68
-1.31
0
0
1
0
1
1
1
1
0
5
5
4
4
4
4
4
4
3
94
96
3.1e+002
2.2e+002
3.2e+002
3.4e+002
1.8e+002
21
4e+002
1
1
1
1
1
1
1
1
1
ALLLSGVIA
HMLTHSDDK + Oxidation (M)
LLYWGSYPRK
NIGCCLCVLM + Acrylamide (C); 2 Carbamidomethyl (C)
ECEKMEEIIGGAR + Carbamidomethyl (C)
ITIDNNMKVDNTYEMK + Oxidation (M)
RVPALSGR
MLTLSAKLK
MHAEIISMGVR
12/9/2009
99
170
157
14
21
4
2
3
9
32
35
589.3433
814.3491
777.8376
414.7615
425.7607
392.7485
362.2222
384.2352
406.2484
450.2744
458.7879
1176.6720
1626.6836
1553.6606
827.5084
849.5068
783.4824
722.4298
766.4558
810.4822
898.5342
915.5612
39
44
112
168
472.2876
480.8009
625.9133
807.3417
942.5606
959.5872
1249.8120
1612.6688
1176.6714
1626.6800
1553.6602
827.5116
849.5072
783.4854
0.54
2.24
0.26
-3.86
-0.45
-3.78
1
0
0
0
1
1
3
3
3
2
1
1
2.6e+002
1.4e+002
2e+002
4.3e+002
4.8e+002
5.7e+002
Page 8 of 8
1
1
1
1
1
1
KGTVTGSVSTIK
CIQTSCQNCLSDGK + Acrylamide (C); Carbamidomethyl (C)
QCAEQFTQNACSPK
LVISAPTK
IVRFSTK
LPAKIDK
Search Parameters
Type of search
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
MS/MS Ion Search
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
200
12/9/2009
Page 1 of 9
User
Email
Search title
MS data file
Database
Timestamp
Protein hits
:
:
:
:
:
:
:
LTQFT Daemon
D:\Data\Mark\18_7-09\N_18_7_09_4.RAW
9 Dec 2009 at 03:59:23 GMT
gi|5902685
RecName: Full=2S albumin; AltName: Full=GM2S-1; Contains: RecName: Full=2S albumin small chain; AltName: Full=Aspartic acidgi|122411
gi|1942778
gi|122614
gi|136429
gi|494583
gi|2266432
gi|157164966 ribosomal protein L9 [Campylobacter concisus 13826]
gi|229552
albumin
gi|28317
gi|158604951 metalloregulation DNA-binding stress protein [Campylobacter concisus 13826]
gi|504
Bovine Lactoferrin [Bos taurus]
gi|154174468 30S ribosomal protein S5 [Campylobacter curvus 525.92]
gi|3914346
gi|118474733 50S ribosomal protein L7/L12 [Campylobacter fetus subsp. fetus 82-40]
gi|157165390 30S ribosomal protein S6 [Campylobacter concisus 13826]
gi|123506
gi|154175471 30S ribosomal protein S6 [Campylobacter curvus 525.92]
gi|538275
18
0
0.00 %
32
0
0.00 %
Format As
Standard scoring
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1.
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Peptide Summary
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MudPIT scoring
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Decreasing Score
Error tolerant
Require bold red
Archive Report
gi|5902685
Mass: 18448
Score: 222
Queries matched: 10
emPAI: 2.84
RecName: Full=2S albumin; AltName: Full=GM2S-1; Contains: RecName: Full=2S albumin small chain; AltName: Full=Aspartic acid-rich peptide; Contains: R
Query
97
123
125
133
135
138
141
144
165
Observed
607.2542
693.8559
700.8637
739.8377
747.8357
749.8130
750.8035
757.8109
867.9149
Mr(expt)
1212.4938
1385.6972
1399.7128
1477.6608
1493.6568
1497.6114
1499.5924
1513.6072
1733.8152
Mr(calc)
1212.4937
1385.6973
1399.7129
1477.6606
1493.6555
1497.6116
1499.5909
1513.6065
1733.8141
0.14
0
42
0.015
1
-0.03
0
(29)
1.4
1
-0.06
0
32
0.64
1
0.18
0
57 0.0014
1
0.90
0
(39) 0.063
1
-0.09
0
50
0.002
1
1.05
0
(24)
0.42
1
0.49
0
(22)
0.92
1
0.65
1
(38)
0.14
1
Peptide
K.CCTEMSELR.S + 2 Acrylamide (C)
K.QLQGVNLTPCEK.H + Carbamidomethyl (C)
K.QLQGVNLTPCEK.H + Acrylamide (C)
K.IMENQSEELEEK.Q
K.IMENQSEELEEK.Q + Oxidation (M)
R.FGPMIQCDLSSDD.- + Acrylamide (C)
R.FGPMIQCDLSSDD.- + Carbamidomethyl (C); Oxidation (M)
R.FGPMIQCDLSSDD.- + Acrylamide (C); Oxidation (M)
K.IMENQSEELEEKQK.K
12/9/2009
167
2.
875.9125
1749.8104
1749.8090
0.81
1
42
0.052
1
Page 2 of 9
K.IMENQSEELEEKQK.K + Oxidation (M)
gi|122411
Mass: 15236
Score: 219
Queries matched: 7
emPAI: 0.22
Query
7
62
Observed
409.7239
529.2728
Mr(expt)
817.4332
1056.5310
Mr(calc)
817.4334
1056.5314
-0.20
0
30
0.62
1
-0.35
0
32
0.63
1
Peptide
R.VDPVNFK.L
67
83
103
146
148
544.3168
573.3298
634.8583
505.9106
758.3630
1086.6190
1144.6450
1267.7020
1514.7100
1514.7114
1086.6186
1144.6452
1267.7024
1514.7113
1514.7113
0.43
-0.11
-0.26
-0.91
0.07
K.LRVDPVNFK.L
M.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
1
1
0
0
0
24
3.9
39
0.12
57 0.0011
38
0.17
(25)
2.8
1
1
1
2
1
gi|230479
Mass: 15105
Score: 219
Queries matched: 7
gi|230632
Mass: 15105
Score: 219
Queries matched: 7
gi|313500
Mass: 15236
Score: 219
Queries matched: 7
Mass: 15105
Score: 219
Queries matched: 7
gi|24158717
3.
gi|1942778
Mass: 15089
Score: 217
Queries matched: 7
emPAI: 0.50
Query
7
62
67
83
103
139
140
Observed
409.7239
529.2728
544.3168
573.3298
634.8583
500.5794
750.3657
Mr(expt)
817.4332
1056.5310
1086.6190
1144.6450
1267.7020
1498.7164
1498.7168
gi|146149083
Mass:
gi|146149272
Mass:
4.
Mr(calc)
817.4334
1056.5314
1086.6186
1144.6452
1267.7024
1498.7164
1498.7164
-0.20
0
30
0.62
1
-0.35
0
32
0.63
1
0.43
1
24
3.9
1
-0.11
1
39
0.12
1
-0.26
0
57 0.0011
1
-0.05
0
(31)
0.74
1
0.27
0
36
0.25
1
15220
Score: 217
caballus]
15220
Score: 217
caballus]
Peptide
R.VDPVNFK.L
K.LRVDPVNFK.L
-.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEFGAEALER.M
K.VGGHAGEFGAEALER.M
Queries matched: 7
Queries matched: 7
gi|122614
Mass: 15998
Score: 148
Queries matched: 4
emPAI: 0.21
Query
85
106
129
186
Observed
575.3405
637.8665
713.8465
1008.4620
Mr(expt)
1148.6664
1273.7184
1425.6784
2014.9094
Mr(calc)
1148.6666
1273.7183
1425.6776
2014.9095
-0.11
0
32
0.23
1
0.12
0
32
0.43
1
0.60
0
37
0.23
1
-0.03
0
47
0.013
1
Peptide
K.VVAGVANALAHK.Y
R.LLVVYPWTQR.F
K.DFTPELQASYQK.V
Mass: 15970
Score: 148
Queries matched: 4
gi|230480
gi|255683515
Mass: 16129
Score: 148
Queries matched: 4
5.
gi|136429
Mass: 24394
Score: 146
Queries matched: 7
emPAI: 0.14
Query
13
14
60
187
188
189
190
Observed
421.7582
421.7583
523.2854
1106.0560
737.7065
737.7065
761.7319
Mr(expt)
841.5018
841.5020
1044.5562
2210.0974
2210.0977
2210.0977
2282.1739
Mr(calc)
841.5022
841.5022
1044.5564
2210.0967
2210.0967
2210.0967
2282.1729
-0.36
0
47
0.035
1
-0.12
0
(43) 0.074
1
-0.10
0
35
0.56
1
0.33
0
(37)
0.26
1
0.43
0
(36)
0.27
1
0.43
0
40
0.12
1
0.43
0
25
3.4
1
Peptide
R.VATVSLPR.S
R.VATVSLPR.S
K.LSSPATLNSR.V
K.IITHPNFNGNTLDNDIMLIK.L
gi|494360
Mass: 23458
Score: 146
Queries matched: 7
gi|999627
Mass: 8814
Score: 146
Queries matched: 7
12/9/2009
Page 3 of 9
gi|1942351
Mass: 13284
Score: 146
Queries matched: 7
gi|2914482
Mass: 23460
Score: 146
Queries matched: 7
gi|3318722
Mass: 23457
Score: 146
Queries matched: 7
gi|110590762
Mass: 23458
Score: 146
Queries matched: 7
gi|157878102
Mass: 24157
Score: 146
Queries matched: 7
gi|242253868
Mass: 25865
Score: 146
Queries matched: 7
6.
gi|158604950
Mass: 13498
Score: 136
Queries matched: 2
emPAI: 0.57
Query
116
156
7.
Observed
660.8356
794.4044
Mr(expt)
1319.6566
1586.7942
Mr(calc)
1319.6568
1586.7940
ppm Miss Score
Expect Rank
-0.15
0
79 1.6e-005
1
0.14
0
57
0.0026
1
Peptide
R.STAAAAAIDSSDLK.I
R.TPSEWQETGIIAGAK.T
gi|494583
Mass: 15714
Score: 135
Queries matched: 4
emPAI: 0.22
Query
9
43
66
193
Observed
414.2710
499.8033
541.7647
905.0785
Mr(expt)
826.5274
997.5920
1081.5148
2712.2137
Mr(calc)
826.5276
997.5920
1081.5152
2712.2151
-0.23
0
33
0.3
1
0.01
0
46
0.019
1
-0.37
0
31
0.67
1
-0.51
0
25
1.8
1
Peptide
-.VQAVAVLK.G
K.LIGPTSVVGR.S
K.THGAPTDEVR.H
K.FEQASESEPTTVSYEIAGNSPNAER.G
Mass: 15845
Score: 135
Queries matched: 4
gi|6322564
gi|6730103
Mass: 15680
Score: 135
Queries matched: 4
gi|15826571
Mass: 15724
Score: 135
Queries matched: 4
gi|27573538
Mass: 15944
Score: 135
Queries matched: 4
gi|27573539
Mass: 15836
Score: 135
Queries matched: 4
Mass: 15811
Score: 135
Queries matched: 4
gi|27573540
8.
gi|2266432
Mass: 59208
Score: 127
Queries matched: 3
emPAI: 0.11
Query
37
Observed
481.2768
Mr(expt)
960.5390
Mr(calc)
960.5393
117
160
663.3477
842.8415
1324.6808
1683.6684
1324.6809
1683.6682
-0.24
0
33
0.87
1
Peptide
K.VSPAVFVSR.E
-0.05
0.15
K.VAALGSDPHISMK.V
R.EGQEQEGEEMAEYR.G
0
0
44
50
0.065
0.0012
1
1
Mass: 59239
Score: 127
Queries matched: 3
gi|3183510
gi|12647311
Mass: 32265
Score: 127
Queries matched: 3
gi|27806233
Mass: 59225
Score: 127
Queries matched: 3
9.
gi|157164966
Mass: 16116
Score: 108
Queries matched: 3
ribosomal protein L9 [Campylobacter concisus 13826]
Query
56
63
96
10.
Observed
513.2846
532.8031
599.2986
gi|229552
albumin
Mr(expt)
1024.5546
1063.5916
1196.5826
Mass: 66088
Mr(calc)
1024.5553
1063.5914
1196.5826
Score: 101
-0.63
0
28
0.6
2
0.28
0
41
0.055
1
0.06
0
38
0.2
1
Queries matched: 2
Peptide
K.AIDLDTHLK.A
K.AVGLYDVSIK.L
K.DGYGNNFLIGK.G
emPAI: 0.05
Query
44
Observed
501.7953
Mr(expt)
1001.5760
Mr(calc)
1001.5757
0.33
0
59 0.0011
1
Peptide
K.LVVSTQTALA.-
12/9/2009
89
582.3192
1162.6238
1162.6234
0.41
0
42
0.084
1
Page 4 of 9
K.LVNELTEFAK.T
gi|1351907
Mass: 69248
Score: 101
Queries matched: 2
Mass: 69278
Score: 101
Queries matched: 2
gi|30794280
gi|74267962
Mass: 69190
Score: 101
Queries matched: 2
gi|154425704
Mass: 69248
Score: 101
Queries matched: 2
11.
gi|28317
Mass: 59492
Score: 98
Queries matched: 2
emPAI: 0.06
Query
68
122
Observed
545.7692
691.3278
Mr(expt)
1089.5238
1380.6410
Mr(calc)
1089.5237
1380.6408
0.15
0
41
0.094
1
0.15
0
57 0.0027
1
Peptide
K.VTMQNLNDR.L
R.ALEESNYELEGK.I
Mass: 57807
Score: 98
Queries matched: 2
gi|387397
epidermal keratin subunit I
Mass: 57213
Score: 98
Queries matched: 2
gi|623409
gi|7638398
Mass: 34962
Score: 98
Queries matched: 2
epidermal keratin 10 [Mus musculus]
Mass: 58587
Score: 98
Queries matched: 2
gi|12852157
gi|21961605
Mass: 58792
Score: 98
Queries matched: 2
gi|26349141
Mass: 52653
Score: 98
Queries matched: 2
Mass: 49471
Score: 98
Queries matched: 2
gi|26349459
gi|61740600
Mass: 57676
Score: 98
Queries matched: 2
gi|87045985
Mass: 31541
Score: 98
Queries matched: 2
keratin 10 [Oryctolagus cuniculus]
gi|109115260
Mass: 62063
Score: 98
Queries matched: 2
gi|109115262
Mass: 57547
Score: 98
Queries matched: 2
gi|109115264
Mass: 56705
Score: 98
Queries matched: 2
gi|112983636
Mass: 57007
Score: 98
Queries matched: 2
keratin complex 1, acidic, gene 10 [Mus musculus]
Mass: 62761
Score: 98
Queries matched: 2
gi|114667509
gi|114667511
Mass: 58244
Score: 98
Queries matched: 2
gi|114667513
Mass: 56692
Score: 98
Queries matched: 2
gi|116242600
Mass: 57735
Score: 98
Queries matched: 2
RecName: Full=Keratin, type I cytoskeletal 10; AltName: Full=Cytokeratin-10; Short=CK-10; AltName: Full=Keratin-10; Short=K10; AltName: Full=56 kDa c
gi|119581085
Mass: 63308
Score: 98
Queries matched: 2
gi|149723838
Mass: 61580
Score: 98
Queries matched: 2
gi|194216982
Mass: 61493
Score: 98
Queries matched: 2
gi|195972866
Mass: 58766
Score: 98
Queries matched: 2
gi|251757513
Mass: 58818
Score: 98
Queries matched: 2
gi|114667507
Mass: 100282
Score: 97
Queries matched: 2
12.
gi|157164793
Mass: 15626
Score: 98
Queries matched: 3
emPAI: 0.48
Query
112
114
137
13.
Observed
645.7870
653.7848
748.3854
Mr(expt)
1289.5594
1305.5550
1494.7562
Mr(calc)
1289.5598
1305.5547
1494.7566
-0.27
0
53 0.0026
1
0.25
0
(42)
0.02
1
-0.22
1
44
0.046
1
Peptide
K.DGTFESVMNYK.S
K.DGTFESVMNYK.S + Oxidation (M)
K.NGVITTVDEYKEK.S
gi|158604951
Mass: 16464
Score: 97
Queries matched: 3
emPAI: 0.45
metalloregulation DNA-binding stress protein [Campylobacter concisus 13826]
12/9/2009
Page 5 of 9
Query
87
170
175
14.
Observed
577.8577
881.3678
889.3652
Mr(expt)
1153.7008
1760.7210
1776.7158
Mr(calc)
1153.7005
1760.7199
1776.7148
gi|504
Mass: 75134
Score: 96
ppm Miss Score
Expect Rank
0.29
0
25
0.28
1
0.65
0
(46)
0.0046
1
0.57
0
72 9.1e-006
1
Queries matched: 3
Peptide
R.ALMLGGRPIVK.A
K.AYEDMSEIFDDAAER.A
K.AYEDMSEIFDDAAER.A + Oxidation (M)
emPAI: 0.04
Query
126
178
179
Observed
701.3445
914.4412
914.4421
Mr(expt)
1400.6744
1826.8678
1826.8696
Mr(calc)
1400.6758
1826.8686
1826.8686
-1.00
0
33
0.45
1
-0.43
0
(35)
0.4
1
0.55
0
62 0.00062
1
Peptide
R.VVWCAVGPEEQK.K + Carbamidomethyl (C)
K.GEADALNLDGGYIYTAGK.C
Mass: 78070
Score: 96
Queries matched: 3
gi|506
lactotransferrin [Bos taurus]
gi|163285
Mass: 77873
Score: 96
Queries matched: 3
lactoferrin
Mass: 78117
Score: 96
Queries matched: 3
gi|408928
lactoferrin
gi|9650974
Mass: 78025
Score: 96
Queries matched: 3
lactoferrin [Bos taurus]
gi|22795592
Mass: 38836
Score: 96
Queries matched: 3
gi|30794292
Mass: 78006
Score: 96
Queries matched: 3
Mass: 37957
Score: 96
Queries matched: 3
gi|34810001
Chain A, Crystal Structure Of A Proteolytically Generated Functional Monoferric C-Lobe
gi|46015771
Mass: 36554
Score: 96
Queries matched: 3
Chain A, Crystal Structure Of The Zinc Saturated C-Terminal Half Of Bovine Lactoferrin
Mass: 37586
Score: 96
Queries matched: 3
gi|83754256
Chain A, Crystal Structure Of The Complex Of C-Lobe Of Bovine Lactoferrin With Maltose
gi|88702501
Mass: 78004
Score: 96
Queries matched: 3
lactoferrin [Bos grunniens]
gi|146157606
Mass: 78129
Score: 96
Queries matched: 3
lactoferrin [Bos indicus x Bos taurus]
Mass: 37439
Score: 96
Queries matched: 3
gi|146387335
Chain A, Crystal Structure Of The C-Terminal Lobe Of Bovine Lactoferrin Complexed With
gi|157830374
Mass: 76095
Score: 96
Queries matched: 3
Chain A, Structure Of Diferric Bovine Lactoferrin At 2.8 Angstroms Resolution
gi|170672383
Mass: 77975
Score: 96
Queries matched: 3
Mass: 78006
Score: 96
Queries matched: 3
gi|170672385
Mass: 78006
Score: 96
Queries matched: 3
gi|193299659
gi|255762013
Mass: 76226
Score: 96
Queries matched: 3
lactoferrin precursor [Bos taurus]
15.
gi|154174468
Mass: 15879
Score: 91
Queries matched: 2
30S ribosomal protein S5 [Campylobacter curvus 525.92]
Of Bovine Lactoferrin At 1.9a Resolution
At 2.0 A Resolution Reveals Two Additional Zinc Binding Sites
At 1.5a Resolution
O-Alpha-D-Glucopyranosyl-(1 3)- Alpha-D-Fructofuranosyl- (2 1)
emPAI: 0.21
Query
50
98
Observed
506.2664
609.3150
Mr(expt)
1010.5182
1216.6154
Mr(calc)
1010.5185
1216.6160
-0.29
0
34
0.58
1
-0.45
0
58 0.0026
1
Peptide
R.NGLVGFGYGK.A
K.SLGSNNSANVVR.A
Mass: 15941
Score: 91
Queries matched: 2
gi|157164159
30S ribosomal protein S5 [Campylobacter concisus 13826]
16.
gi|3914346
Mass: 82383
Score: 86
Queries matched: 3
Query
35
38
94
17.
Observed
478.7740
488.2789
596.8193
Mr(expt)
955.5334
974.5432
1191.6240
Mr(calc)
955.5338
974.5437
1191.6248
-0.41
0
23
3.9
1
-0.43
0
27
3.8
1
-0.59
1
36
0.31
1
Peptide
K.VVQGEPSLK.V
R.WISTVELK.V
R.GLIKEQYEGR.L
gi|157164111
Mass: 16254
Score: 71
Queries matched: 1
emPAI: 0.21
Query
Observed
Mr(expt)
Mr(calc)
ppm
Miss Score
Expect Rank
Peptide
12/9/2009
142
18.
751.3619
1500.7092
gi|27806789
Mass: 15717
1500.7096
-0.26
Score: 69
0
71
5.7e-005
Queries matched: 2
Page 6 of 9
1
K.DGSQEVFATYIGSK.K
emPAI: 0.22
Query
25
127
Observed
450.2509
704.8234
Mr(expt)
898.4872
1407.6322
Mr(calc)
898.4872
1407.6306
0.02
0
28
1.4
1
1.14
0
42
0.046
1
Peptide
R.GSPAANVGVK.V
K.AADETWEPFASGK.T
gi|57526651
Mass: 15761
Score: 69
Queries matched: 2
19.
gi|118474733
Mass: 12992
Score: 65
Queries matched: 2
50S ribosomal protein L7/L12 [Campylobacter fetus subsp. fetus 82-40]
Query
74
130
Observed
558.3007
722.3878
Mr(expt)
1114.5868
1442.7610
Mass:
gi|154174384
50S ribosomal protein
gi|157165629
Mass:
50S ribosomal protein
20.
Mr(calc)
1114.5870
1442.7617
-0.14
0
44
0.036
1
-0.42
1
21
8.4
1
Peptide
K.DAVEGTPSVLK.E
K.EAKDAVEGTPSVLK.E
13079
Score: 65
Queries matched: 2
L7/L12 [Campylobacter curvus 525.92]
13007
Score: 65
Queries matched: 2
L7/L12 [Campylobacter concisus 13826]
gi|157165390
Mass: 16384
Score: 59
Queries matched: 1
emPAI: 0.21
Query
86
21.
Observed
576.3426
Mr(expt)
1150.6706
Mr(calc)
1150.6710
-0.28
0
59 0.00028
1
Peptide
K.APPALLAELTR.N
gi|123506
Mass: 8353
Score: 51
Queries matched: 1
emPAI: 0.42
Query
39
Observed
492.3034
Mr(expt)
982.5922
Mr(calc)
982.5923
-0.08
0
51 0.0027
1
Peptide
R.ALGILNLNR.N
gi|5019730
Mass: 12474
Score: 51
Queries matched: 1
gi|76782253
Mass: 12343
Score: 51
Queries matched: 1
22.
gi|154175471
Mass: 16754
Score: 51
Queries matched: 1
30S ribosomal protein S6 [Campylobacter curvus 525.92]
emPAI: 0.20
Query
131
23.
Observed
725.3539
Mr(expt)
1448.6932
Mr(calc)
1448.6929
gi|538275
Mass: 22697
Score: 51
0.21
0
51 0.0077
1
Queries matched: 1
Peptide
K.NGGEIATVVEMGTR.K + Oxidation (M)
emPAI: 0.15
Query
75
Observed
561.3134
Mr(expt)
1120.6122
Mr(calc)
1120.6128
-0.51
0
51 0.0078
1
Peptide
K.DTQSIIFLGK.V
gi|1245695
Mass: 28553
Score: 51
Queries matched: 1
gi|1245697
Mass: 9788
Score: 51
Queries matched: 1
gi|31340900
Mass: 46175
Score: 51
Queries matched: 1
gi|84000377
Mass: 46297
Score: 51
Queries matched: 1
gi|121531628
Mass: 46282
Score: 51
Queries matched: 1
gi|126165236
Mass: 46368
Score: 51
Queries matched: 1
12/9/2009
gi|126165264
Mass: 46888
Score: 51
Queries matched:
gi|151554250
Mass: 46935
Score: 51
Queries matched:
gi|160332365
Mass: 46208
Score: 51
Queries matched:
RecName: Full=Serpin A3-1; AltName: Full=Endopin-1A; AltName:
gi|194038345
Mass: 38368
Score: 51
Queries matched:
Mass: 47192
Score: 51
Queries matched:
gi|194038353
Mass: 46208
Score: 51
Queries matched:
gi|226373737
gi|226373739
Mass: 46361
Score: 51
Queries matched:
Page 7 of 9
1
1
1
Full=Muscle endopin-1A; Short=mEndopin-1A; Flags: Precursor
1
1
1
1
Query
53
146
79
70
132
57
95
54
154
56
21
52
120
69
136
80
119
47
93
100
29
8
15
23
159
46
153
27
64
12
107
169
101
30
49
134
32
58
108
168
113
147
61
82
51
173
34
174
19
91
6
20
48
77
88
143
172
128
92
90
Observed
508.7722
505.9106
564.8528
546.2900
738.3962
514.8025
597.3248
510.7713
783.8737
513.2846
435.7739
508.7479
681.3486
545.7946
748.3268
565.7697
681.3364
503.7636
596.3179
617.8560
466.2944
410.7269
421.7585
442.7579
834.9258
503.7406
783.8732
453.7497
534.2762
421.7400
639.3041
875.9437
627.2768
467.7310
505.8156
740.3287
475.7139
516.3027
427.5894
584.2974
650.3320
758.3628
526.2641
382.5553
507.8134
589.6297
478.2534
886.3577
430.2476
585.2885
407.2287
432.7492
505.2692
563.7855
581.7906
752.8676
883.9409
713.3224
585.8552
582.8109
Mr(expt)
1015.5298
1514.7100
1127.6910
1090.5654
1474.7778
1027.5904
1192.6350
1019.5280
1565.7328
1024.5546
869.5332
1015.4812
1360.6826
1089.5746
1494.6390
1129.5248
1360.6582
1005.5126
1190.6212
1233.6974
930.5742
819.4392
841.5024
883.5012
1667.8370
1005.4666
1565.7318
905.4848
1066.5378
841.4654
1276.5936
1749.8728
1252.5390
933.4474
1009.6166
1478.6428
949.4132
1030.5908
1279.7464
1749.8704
1298.6494
1514.7110
1050.5136
1144.6441
1013.6122
1765.8673
954.4922
1770.7008
858.4806
1168.5624
812.4428
863.4838
1008.5238
1125.5564
1161.5666
1503.7206
1765.8672
1424.6302
1169.6958
1163.6072
Mr(calc)
1015.5298
1514.7113
1127.6914
1090.5659
1474.7780
1027.5913
1192.6353
1019.5301
1565.7283
1024.5553
869.5334
1015.4822
1360.6843
1089.5740
1494.6404
1129.5251
1360.6583
1005.5165
1190.6217
1233.6969
930.5750
819.4425
841.5022
883.5015
1667.8366
1005.4668
1565.7283
905.4827
1066.5342
841.4657
1276.5904
1749.8719
1252.5434
933.4477
1009.6172
1478.6421
949.4109
1030.5910
1279.7500
1749.8719
1298.6507
1514.7113
1050.5128
1144.6452
1013.6121
1765.8709
954.4957
1770.7045
858.4824
1168.5625
812.4432
863.4865
1008.5240
1125.5562
1161.5675
1503.7214
1765.8669
1424.6307
1169.6954
1163.6074
ppm Miss Score
0.03
0
48
-0.91
0
42
-0.30
0
39
-0.38
0
36
-0.09
0
36
-0.87
0
36
-0.21
0
36
-2.00
0
35
2.90
1
34
-0.62
0
33
-0.23
0
32
-0.92
0
32
-1.19
1
31
0.60
0
30
-0.91
0
29
-0.25
0
28
-0.01
1
28
-3.81
0
27
-0.34
0
27
0.46
0
26
-0.77
0
26
-3.97
1
26
0.35
0
25
-0.25
0
24
0.26
0
24
-0.19
0
24
2.26
1
23
2.39
0
22
3.45
1
22
-0.33
0
22
2.56
0
22
0.51
0
21
-3.48
0
21
-0.29
0
20
-0.50
0
20
0.49
0
20
2.42
0
20
-0.17
0
19
-2.82
1
19
-0.90
0
18
-0.94
0
18
-0.20
0
18
0.82
0
18
-0.96
1
18
0.17
0
17
-2.07
0
17
-3.58
0
17
-2.07
1
16
-2.03
1
16
-0.07
0
16
-0.48
0
16
-3.08
0
15
-0.14
1
15
0.19
0
15
-0.70
0
15
-0.48
0
14
0.22
0
14
-0.32
0
14
0.35
0
14
-0.12
0
13
Expect Rank
0.03
1
0.057
1
0.041
1
0.41
1
0.26
1
0.22
1
0.36
1
0.63
1
0.39
1
0.19
1
0.75
1
0.37
1
1.4
1
1.1
1
0.53
1
0.91
1
1.7
1
3.8
1
1.8
1
1.5
1
0.8
1
1.7
1
4.8
1
2
1
4.4
1
3.7
1
5.9
1
7.2
1
7.6
1
6.3
1
6.7
1
10
1
4.6
1
9.7
1
1.6
1
4.1
1
4.5
1
6.8
1
2.9
1
20
1
13
1
12
1
16
1
13
1
10
1
25
1
16
1
2.2
1
55
1
28
1
13
1
52
1
31
1
41
1
54
1
42
1
52
1
29
1
12
1
48
1
Peptide
DALSIQDVR
VGAHAGDYGAEALER
IITEVATILR
AEFIQAVADK
FLEQQNQVLQTK
AQVLEIVEK
QFQFIQVAGR
SLHHNVWK
AIDLISEHK
VVSLSIPR
TPELEENGK
RIEMCPDIIQK + Oxidation (M)
MLDVQDILK + Oxidation (M)
MEMPTEQEMLPK + 2 Oxidation (M)
DQPTIEQGDK
SGSDEVQVKQER
GQMIEVSVK + Oxidation (M)
IVEIPGEVPPGK
SILVATLSK
ALWKMR + Oxidation (M)
LGSLTVRP
VVELEPAK
IIPSAEDPSQDIVER
SVDWTGWR
MSVCVVLR
RCSAAAGYLR
AATAAAAAPK
DTKPGTNNSAIFMNIK
EPDPNAFYGLM
AEVALPLAVK
MEIDLCSYSGYK + Acrylamide (C)
VLDELTLTK
VVQGEPSLKVPK
DTKPGTNNSAIFMNIK
YFSQVDIVSNK
VGGHAGEYGAEALER
CSTLTISNR + Carbamidomethyl (C)
VLSAADKTNVK
QTALVELLK
TGAGFSMLLVDLDHFK + Oxidation (M)
NPCDLDLCKDSCCTK + 2 Carbamidomethyl (C)
WRASAIR
IQHQDWTGGK
SVVYFAK
GVTLGVYR
ETLASYGRL
MGLYDGVMIK
ACLPWQMVK + Acrylamide (C); Oxidation (M)
EFTTEADIGGEEK
ALMLGGRPIVK + Oxidation (M)
VLDEIGVEYK
12/9/2009
Page 8 of 9
177
109
163
118
180
73
110
166
115
164
897.9113
640.8824
857.4078
671.3452
621.6437
556.3163
641.8485
584.2771
655.3230
578.9454
1793.8080
1279.7502
1712.8010
1340.6758
1861.9093
1110.6180
1281.6824
1749.8095
1308.6314
1733.8144
1793.8116
1279.7500
1712.8006
1340.6758
1861.9026
1110.6186
1281.6789
1749.8090
1308.6310
1733.8141
-2.01
0.21
0.28
0.02
3.59
-0.46
2.75
0.25
0.38
0.15
0
0
0
0
0
0
1
1
1
1
13
13
13
13
13
13
13
13
13
13
54
10
40
49
59
38
46
50
57
54
1
1
1
1
1
1
1
1
1
1
MDAIIFASYCEFAAR + Acrylamide (C); Oxidation (M)
LTAGVLVNAPTPK
YLDISDGNLQEGSFR
VAALGSDPHISMK + Oxidation (M)
MIQMETILNVADNSGAR
AIASFLAGHPK
NNGPDRLVLER
IMENQSEELEEKQK + Oxidation (M)
ARNDEYEIATK
IMENQSEELEEKQK
181
76
42
99
11
161
171
33
24
191
155
124
81
158
626.9750
563.7853
496.7979
610.7983
420.7811
842.8996
883.8956
475.7286
448.7182
767.0629
785.8728
695.8459
566.2979
797.9089
1877.9032
1125.5560
991.5812
1219.5820
839.5476
1683.7846
1765.7766
949.4426
895.4218
2298.1669
1569.7310
1389.6772
1130.5812
1593.8032
1877.8975
1125.5601
991.5815
1219.5833
839.5480
1683.7886
1765.7802
949.4440
895.4222
2298.1678
1569.7344
1389.6736
1130.5819
1593.7987
3.01
-3.58
-0.22
-1.04
-0.42
-2.37
-2.00
-1.39
-0.37
-0.40
-2.16
2.65
-0.57
2.86
1
0
0
1
1
1
1
1
0
0
1
1
0
1
13
12
12
12
12
12
12
11
11
10
10
9
9
9
60
66
23
74
11
76
39
54
96
1e+002
1e+002
1.5e+002
1.1e+002
1.5e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
IMEETTAKTISHCSVR + Carbamidomethyl (C); Oxidation (M)
MGDVEVHALR
QTHGKPLLV
GKDAGFAPGDSAK
IIIPKEK
MTKDSPSPSGSGHATPK
WECVSGSGTKNSNQNR
MFDPRER
LSTYCPR + Carbamidomethyl (C)
IITHPNFNGNTLDNDIMLIK + Oxidation (M)
TIIIEDNRSTDN
IATESEGSPLK
VGHPFRHGIWCSK + Acrylamide (C)
183
192
78
41
72
55
111
84
162
157
105
185
4
17
18
104
16
71
184
151
176
152
149
22
102
182
121
150
970.9703
829.0640
564.2778
496.7213
555.7513
512.2409
642.3398
574.2834
843.3915
794.8651
635.7662
1001.0730
399.1929
428.2615
428.7786
635.2747
425.7609
550.2451
972.9594
769.3242
894.4135
777.8377
766.3239
436.7748
627.7692
964.9624
454.5683
768.3484
1939.9260
2484.1702
1126.5410
991.4280
1109.4880
1022.4672
1282.6650
1146.5522
1684.7684
1587.7156
1269.5178
2000.1314
796.3712
854.5084
855.5426
1268.5348
849.5072
1098.4756
1943.9042
1536.6338
1786.8124
1553.6608
1530.6332
871.5350
1253.5238
1927.9102
1360.6831
1534.6822
1939.9244
2484.1690
1126.5416
991.4319
1109.4845
1022.4703
1282.6629
1146.5517
1684.7726
1587.7174
1269.5152
2000.1306
796.3715
854.5086
855.5430
1268.5343
849.5072
1098.4764
1943.9081
1536.6306
1786.8171
1553.6603
1530.6377
871.5378
1253.5203
1927.9165
1360.6847
1534.6794
0.85
0.48
-0.47
-3.87
3.17
-2.95
1.64
0.52
-2.48
-1.09
2.11
0.44
-0.36
-0.20
-0.36
0.43
0.02
-0.68
-1.99
2.14
-2.62
0.38
-2.94
-3.21
2.85
-3.25
-1.22
1.84
1
1
1
1
1
0
0
0
1
0
0
1
0
1
0
0
1
1
1
0
0
0
0
1
0
1
0
1
9
9
9
9
9
9
8
8
8
8
8
7
7
7
7
7
7
6
6
6
6
5
5
5
5
4
4
3
1.5e+002
1.2e+002
1.2e+002
45
1e+002
1.9e+002
1.1e+002
1.8e+002
1.3e+002
1.2e+002
35
48
94
81
61
87
1.2e+002
69
2e+002
43
2e+002
1.1e+002
65
1.8e+002
1.2e+002
4.2e+002
6.9e+002
2.7e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
TCRVVLASTQCEADAFAR
KLDELAEGDTTTQMYAQDQIAK + Oxidation (M)
MFWAMRTGK
SRGEGEDSR
MDLDKSCGGK + Carbamidomethyl (C)
TSMGDQIGSK
QSNVHLTSEIR
AEVEAGSSELR
DGKNYQMASNTLDTK
YIHHITCGDCLEK + Carbamidomethyl (C)
TIGGMMADCAER + Oxidation (M)
ISRPILDINEKGEIYIK
SGTDYVR
ARGLSVPR
ALVVVEVK
YDCAVLDEDR + Acrylamide (C)
KTSFLVR
NFDKCSETR
YDTDKVAIMITQTGGGCR + Oxidation (M)
VEGMSCMHCQLR + Acrylamide (C); Carbamidomethyl (C); Oxidation (M)
HVHEDVMPFTCTFPK
SFGDMLQAACDPQR + Oxidation (M)
MSTHCCAASSPPPR + Acrylamide (C); Oxidation (M)
ELKGILSL
DVVPMSGMCSR + Carbamidomethyl (C); Oxidation (M)
TLLAMSSSQESCIAMRK + Carbamidomethyl (C); Oxidation (M)
NVHDAAHSLLER
ASSDRPTGRGGVDCS + Acrylamide (C)
28
26
1
2
3
5
10
31
36
40
45
59
65
145
458.7876
450.2746
357.6369
362.2220
384.2353
406.2482
414.7616
472.2876
480.8011
494.3008
502.8140
516.3134
357.2998
758.3265
915.5606
898.5346
713.2592
722.4294
766.4560
810.4818
827.5086
942.5606
959.5876
986.5870
1003.6134
1030.6122
1068.8776
1514.6384
915.5575
898.5348
3.39
-0.21
1
1
2
2
1.9e+002
8.6e+002
1
1
IIVGQMKK
KGGQGIIAR
Search Parameters
Type of search
:
Enzyme
:
Mass values
:
Protein Mass
:
MS/MS Ion Search
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
12/9/2009
Instrument type
Number of queries
Page 9 of 9
: 1
: ESI-TRAP
: 193
12/9/2009
Page 1 of 15
User
Email
Search title
MS data file
Database
Timestamp
Protein hits
:
:
:
:
:
:
:
LTQFT Daemon
D:\Data\Mark\18_7-09\N_18_7_09_5.RAW
9 Dec 2009 at 04:01:45 GMT
gi|157165326 copper/zinc superoxide dismutase [Campylobacter concisus 13826]
gi|5902685
RecName: Full=2S albumin; AltName: Full=GM2S-1; Contains: RecName: Full=2S albumin small chain; AltName: Full=Aspartic acidgi|136429
gi|494583
gi|158604951 metalloregulation DNA-binding stress protein [Campylobacter concisus 13826]
gi|157165180 putative cytochrome C-type haem-binding periplasmic protein [Campylobacter concisus 13826]
gi|229552
albumin
gi|2194088
Chain A, Bovine Beta-Lactoglobulin, Lattice X
gi|34538498 immunoglobulin heavy chain constant region [Bos taurus]
gi|1942778
gi|122411
gi|2266432
gi|157164220 50S ribosomal protein L10 [Campylobacter concisus 13826]
gi|28317
gi|6320157
gi|157165665 outer-membrane lipoprotein carrier protein [Campylobacter concisus 13826]
gi|2232299
gi|7331218
gi|3914346
gi|181402
gi|122614
gi|157165156 thiol peroxidase (scavengase P20) [Campylobacter concisus 13826]
gi|157164172 flavodoxin FldA [Campylobacter concisus 13826]
gi|57169062 ribosomal protein L13 [Campylobacter coli RM2228]
gi|504
gi|158604982 phosphoribosylaminoimidazole carboxylase, catalytic subunit [Campylobacter concisus 13826]
gi|122064583 RecName: Full=Superoxide dismutase [Cu-Zn]
gi|538275
gi|115495209 zymogen granule membrane glycoprotein 2 [Bos taurus]
gi|123506
gi|67408
lysozyme (EC 3.2.1.17) c precursor [validated] - ring-necked pheasant (tentative sequence)
38
0
0.00 %
76
0
0.00 %
Format As
Help
Peptide Summary
Significance threshold p<
Standard scoring
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0.01
MudPIT scoring
Suppress pop-ups
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Max. number of hits
AUTO
Show sub-sets 0
Require bold red
Error tolerant
Archive Report
12/9/2009
1.
gi|157165326
Mass: 18857
Score: 301
Queries matched: 10
copper/zinc superoxide dismutase [Campylobacter concisus 13826]
Page 2 of 15
emPAI: 0.93
Query
32
47
71
157
158
163
220
401
405
409
2.
Observed
425.7298
444.2633
486.7897
584.7725
584.7727
396.5593
685.8586
772.7033
777.3745
777.3749
Mr(expt)
849.4450
886.5120
971.5648
1167.5304
1167.5308
1186.6561
1369.7026
2315.0881
2329.1017
2329.1029
Mr(calc)
849.4452
886.5124
971.5651
1167.5309
1167.5309
1186.6558
1369.7030
2315.0866
2329.1022
2329.1022
ppm Miss Score
Expect Rank
-0.20
0
28
2.2
1
-0.39
0
75 5.8e-005
1
-0.27
1
44
0.048
1
-0.37
0
40
0.1
1
-0.03
0
(31)
0.83
1
0.26
1
27
3.7
1
-0.28
0
58
0.002
1
0.65
0
(22)
6.8
1
-0.23
0
29
1.4
1
0.29
0
(28)
1.9
1
Peptide
R.MLCGVIK.- + Acrylamide (C); Oxidation (M)
K.SVGEVVAVK.T
K.IKNLDELK.G
K.AGGHWDPAETK.M
K.AGGHWDPAETK.M
K.GDKSVGEVVAVK.T
K.TNYGVAFFPNLK.G
K.GLPAGLHGFHVHQNADCGATEK.G + Carbamidomethyl (C)
K.GLPAGLHGFHVHQNADCGATEK.G + Acrylamide (C)
K.GLPAGLHGFHVHQNADCGATEK.G + Acrylamide (C)
gi|5902685
Mass: 18448
Score: 285
Queries matched: 14
emPAI: 2.84
RecName: Full=2S albumin; AltName: Full=GM2S-1; Contains: RecName: Full=2S albumin small chain; AltName: Full=Aspartic acid-rich peptide; Contains: R
Query
169
172
175
226
230
245
246
249
252
256
264
268
317
322
3.
Observed
600.2465
607.2539
610.8099
693.8560
700.8640
739.8379
742.8049
747.8352
749.8129
750.8034
757.8113
763.3441
867.9144
875.9114
Mr(expt)
1198.4784
1212.4932
1219.6052
1385.6974
1399.7134
1477.6612
1483.5952
1493.6558
1497.6112
1499.5922
1513.6080
1524.6736
1733.8142
1749.8082
Mr(calc)
1198.4780
1212.4937
1219.6053
1385.6973
1399.7129
1477.6606
1483.5959
1493.6555
1497.6116
1499.5909
1513.6065
1524.6734
1733.8141
1749.8090
0.34
0
(22)
1.5
1
-0.36
0
44 0.0088
1
-0.03
0
42
0.083
1
0.11
0
36
0.25
1
0.37
0
(25)
3.2
1
0.45
0
57 0.0014
1
-0.47
0
(29)
0.17
1
0.23
0
(37) 0.097
1
-0.23
0
49 0.0024
1
0.92
0
(22)
0.69
1
1.02
0
(33) 0.071
1
0.14
1
25
1.5
1
0.08
1
33
0.51
1
-0.45
1
(26)
2.2
1
gi|136429
Mass: 24394
Score: 219
Queries matched: 12
Peptide
K.CCTEMSELR.S + Acrylamide (C); Carbamidomethyl (C)
K.CCTEMSELR.S + 2 Acrylamide (C)
K.ELINLATMCR.F + Carbamidomethyl (C)
K.QLQGVNLTPCEK.H + Carbamidomethyl (C)
K.QLQGVNLTPCEK.H + Acrylamide (C)
K.IMENQSEELEEK.Q
R.FGPMIQCDLSSDD.- + Carbamidomethyl (C)
K.IMENQSEELEEK.Q + Oxidation (M)
R.FGPMIQCDLSSDD.- + Acrylamide (C)
R.FGPMIQCDLSSDD.- + Carbamidomethyl (C); Oxidation (M)
R.FGPMIQCDLSSDD.- + Acrylamide (C); Oxidation (M)
K.CCTEMSELRSPK.C + 2 Acrylamide (C)
K.IMENQSEELEEKQK.K
K.IMENQSEELEEKQK.K + Oxidation (M)
emPAI: 0.67
Query
24
25
26
28
108
109
269
387
388
389
390
391
Observed
421.7582
421.7582
421.7582
421.7584
523.2854
523.2854
763.8796
737.7062
1106.0560
1106.0560
737.7065
737.7067
Mr(expt)
841.5018
841.5018
841.5018
841.5022
1044.5562
1044.5562
1525.7446
2210.0968
2210.0974
2210.0974
2210.0977
2210.0983
Mr(calc)
841.5022
841.5022
841.5022
841.5022
1044.5564
1044.5564
1525.7446
2210.0967
2210.0967
2210.0967
2210.0967
2210.0967
ppm Miss Score
Expect Rank
-0.36
0
(42)
0.09
1
-0.36
0
(55)
0.0051
1
-0.36
0
56
0.0042
1
0.12
0
(27)
3.3
2
-0.10
0
51
0.014
1
-0.10
0
(27)
3.8
1
0.01
0
23
5.2
1
0.02
0
(59)
0.0014
1
0.33
0
89 1.6e-006
1
0.33
0
(62)
0.0007
1
0.43
0
(30)
1.3
1
0.70
0
(44)
0.05
1
Peptide
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
K.LSSPATLNSR.V
K.LSSPATLNSR.V
gi|494360
Mass: 23458
Score: 219
Queries matched: 12
gi|2914482
Mass: 23460
Score: 219
Queries matched: 12
gi|3318722
Mass: 23457
Score: 219
Queries matched: 12
gi|110590762
Mass: 23458
Score: 219
Queries matched: 12
gi|242253868
Mass: 25865
Score: 219
Queries matched: 12
4.
gi|494583
Mass: 15714
Score: 203
Queries matched: 7
emPAI: 0.48
Query
19
82
83
84
85
Observed
414.2710
497.7818
497.7820
499.8031
499.8034
Mr(expt)
826.5274
993.5490
993.5494
997.5916
997.5922
Mr(calc)
826.5276
993.5495
993.5495
997.5920
997.5920
-0.23
0
29
0.89
1
-0.44
1
46
0.021
1
-0.04
1
(44)
0.037
1
-0.39
0
66 0.00018
1
0.21
0
(30)
0.76
1
Peptide
-.VQAVAVLK.G
K.GSFKDSLIK.L
K.GSFKDSLIK.L
K.LIGPTSVVGR.S
K.LIGPTSVVGR.S
12/9/2009
120
386
541.7648
733.3696
1081.5150
2197.0870
1081.5152
2197.0863
-0.18
0.32
0
1
37
25
0.19
3.2
Page 3 of 15
1
1
K.THGAPTDEVR.H
R.SVVIHAGQDDLGKGDTEESLK.T
gi|6322564
Mass: 15845
Score: 203
Queries matched: 7
Mass: 15680
Score: 203
Queries matched: 7
gi|6730103
gi|15826571
Mass: 15724
Score: 203
Queries matched: 7
gi|27573538
Mass: 15944
Score: 203
Queries matched: 7
gi|27573539
Mass: 15836
Score: 203
Queries matched: 7
gi|27573540
Mass: 15811
Score: 203
Queries matched: 7
5.
gi|158604951
Mass: 16464
Score: 201
Queries matched: 5
emPAI: 1.12
metalloregulation DNA-binding stress protein [Campylobacter concisus 13826]
Query
151
180
324
330
414
6.
Observed
577.8570
617.3364
881.3670
889.3640
829.0643
Mr(expt)
1153.6994
1232.6582
1760.7194
1776.7134
2484.1711
Mr(calc)
1153.7005
1232.6587
1760.7199
1776.7148
2484.1690
ppm Miss Score
Expect Rank
-0.93
0
31
0.075
1
-0.37
0
55
0.0047
1
-0.26
0
94 6.9e-008
1
-0.78
0
(77) 2.3e-006
1
0.84
1
22
6.3
1
Peptide
R.ALMLGGRPIVK.A
K.AIWMLNATLGK.- + Oxidation (M)
K.AYEDMSEIFDDAAER.A
K.AYEDMSEIFDDAAER.A + Oxidation (M)
K.KLDELAEGDTTTQMYAQDQIAK.F + Oxidation (M)
gi|157165180
Mass: 20941
Score: 193
Queries matched: 4
emPAI: 0.56
putative cytochrome C-type haem-binding periplasmic protein [Campylobacter concisus 13826]
Query
31
313
314
358
7.
Observed
424.7656
576.3161
863.9706
964.4682
gi|229552
albumin
Mr(expt)
847.5166
1725.9265
1725.9266
1926.9218
Mass: 66088
Mr(calc)
847.5167
1725.9261
1725.9261
1926.9224
Score: 192
ppm Miss Score
Expect Rank
-0.09
0
40
0.071
1
0.21
0
(26)
1.9
1
0.32
0
74 2.6e-005
1
-0.30
0
79 1.6e-005
1
Queries matched: 5
Peptide
R.IISVAFAK.T
R.LLPTNAVEILQTSGDR.A
K.GYQNPAVSNVVYFADGAR.I
emPAI: 0.10
Query
87
93
141
210
295
Observed
501.7952
507.8131
571.8608
653.3609
820.4727
Mr(expt)
1001.5758
1013.6116
1141.7070
1304.7072
1638.9308
Mr(calc)
1001.5757
1013.6121
1141.7070
1304.7088
1638.9305
0.13
0
54 0.0034
1
-0.42
0
41
0.042
1
0.01
1
27
0.41
1
-1.21
0
31
0.85
1
0.22
1
39
0.034
1
Peptide
K.KQTALVELLK.H
K.HLVDEPQNLIK.Z
R.KVPQVSTPTLVEVSR.S
Mass: 69248
Score: 192
Queries matched: 5
gi|1351907
gi|30794280
Mass: 69278
Score: 192
Queries matched: 5
gi|74267962
Mass: 69190
Score: 192
Queries matched: 5
gi|76445989
Mass: 53890
Score: 192
Queries matched: 5
serum albumin [Bos indicus]
gi|154425704
Mass: 69248
Score: 192
Queries matched: 5
8.
gi|2194088
Mass: 18297
Score: 181
Queries matched: 9
Chain A, Bovine Beta-Lactoglobulin, Lattice X
emPAI: 0.97
Query
34
57
61
168
291
292
293
311
315
Observed
427.2391
467.2751
475.2729
597.3422
545.9293
818.3911
818.3915
858.4061
865.4138
Mr(expt)
852.4636
932.5356
948.5312
1192.6698
1634.7661
1634.7676
1634.7684
1714.7976
1728.8130
Mr(calc)
852.4640
932.5365
948.5314
1192.6703
1634.7675
1634.7675
1634.7675
1714.7985
1728.8141
-0.38
0
27
0.66
3
-0.90
0
(26)
2.5
1
-0.16
0
26
3.4
3
-0.41
1
22
2.7
1
-0.89
1
(33)
0.47
1
0.07
1
50
0.009
1
0.56
1
(30)
0.92
1
-0.47
0
55 0.0026
1
-0.61
0
(53) 0.0054
1
Peptide
K.ALPMHIR.L + Oxidation (M)
-.LIVTQTMK.G
-.LIVTQTMK.G + Oxidation (M)
K.VLVLDTDYKK.Y
R.TPEVDDEALEKFDK.A
R.TPEVDDEALEKFDK.A
R.TPEVDDEALEKFDK.A
R.LSFNPTQLEEQCHI.- + Carbamidomethyl (C)
R.LSFNPTQLEEQCHI.- + Acrylamide (C)
12/9/2009
Page 4 of 15
gi|6729725
Mass: 18269
Score: 181
Queries matched: 9
Chain A, Structural And Functional Consequences Of Point Mutations Of Variants A And B Of Bovine Beta-Lactoglobulin
gi|6980895
Mass: 18355
Score: 181
Queries matched: 9
Chain A, 12-Bromododecanoic Acid Binds Inside The Calyx Of Bovine Beta-Lactoglobulin
Mass: 18339
Score: 181
Queries matched: 9
gi|49259423
Chain X, The Cys121ser Mutant Of Beta-Lactoglobulin
9.
gi|34538498
Mass: 49909
Score: 176
Queries matched: 6
emPAI: 0.14
Query
122
127
197
331
394
395
10.
Observed
547.2811
554.2888
633.8091
892.4928
745.3935
1117.5870
Mr(expt)
1092.5476
1106.5630
1265.6036
1782.9710
2233.1587
2233.1594
Mr(calc)
1092.5485
1106.5641
1265.6040
1782.9702
2233.1590
2233.1590
ppm Miss Score
Expect Rank
-0.79
0
(27)
2.7
1
-0.99
0
30
1.4
2
-0.30
0
37
0.27
1
0.47
0
28
1.1
1
-0.17
0
(32)
0.45
1
0.18
0
81 6.4e-006
1
Peptide
R.ESASVTCLVK.G + Carbamidomethyl (C)
R.ESASVTCLVK.G + Acrylamide (C)
K.ALETHTYFER.H
K.DVAMKPPSVYLLPPTR.E
gi|1942778
Mass: 15089
Score: 174
Queries matched: 8
emPAI: 0.50
Query
107
112
113
121
145
198
253
254
Observed
521.2753
529.2729
529.2729
544.3169
573.3297
634.8586
750.3651
500.5793
Mr(expt)
1040.5360
1056.5312
1056.5312
1086.6192
1144.6448
1267.7026
1498.7156
1498.7161
gi|146149083
Mass:
gi|146149272
Mass:
11.
Mr(calc)
1040.5365
1056.5314
1056.5314
1086.6186
1144.6452
1267.7024
1498.7164
1498.7164
-0.43
0
31
0.58
1
-0.16
0
(31)
0.76
1
-0.16
0
(28)
1.5
1
0.62
1
21
7.6
1
-0.28
1
42
0.062
1
0.21
0
37
0.12
1
-0.53
0
43
0.049
1
-0.25
0
(24)
3.7
1
15220
Score: 174
caballus]
15220
Score: 174
caballus]
Peptide
R.MFLGFPTTK.T
K.LRVDPVNFK.L
-.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEFGAEALER.M
K.VGGHAGEFGAEALER.M
Queries matched: 8
Queries matched: 8
gi|122411
Mass: 15236
Score: 169
Queries matched: 8
emPAI: 0.22
Query
107
112
113
121
145
198
266
267
Observed
521.2753
529.2729
529.2729
544.3169
573.3297
634.8586
505.9110
758.3629
Mr(expt)
1040.5360
1056.5312
1056.5312
1086.6192
1144.6448
1267.7026
1514.7112
1514.7112
Mr(calc)
1040.5365
1056.5314
1056.5314
1086.6186
1144.6452
1267.7024
1514.7113
1514.7113
-0.43
0
31
0.58
1
-0.16
0
(31)
0.76
1
-0.16
0
(28)
1.5
1
0.62
1
21
7.6
1
-0.28
1
42
0.062
1
0.21
0
37
0.12
1
-0.12
0
39
0.11
2
-0.07
0
(29)
0.95
1
Peptide
R.MFLGFPTTK.T
K.LRVDPVNFK.L
M.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
gi|230479
Mass: 15105
Score: 169
Queries matched: 8
gi|230632
Mass: 15105
Score: 169
Queries matched: 8
gi|313500
Mass: 15236
Score: 169
Queries matched: 8
gi|24158717
Mass: 15105
Score: 169
Queries matched: 8
12.
gi|2266432
Mass: 59208
Score: 163
Queries matched: 7
emPAI: 0.18
Query
69
215
302
303
304
305
350
Observed
481.2766
663.3478
840.9489
560.9684
840.9492
560.9686
633.2707
Mr(expt)
960.5386
1324.6810
1679.8832
1679.8834
1679.8838
1679.8840
1896.7903
Mr(calc)
960.5393
1324.6809
1679.8842
1679.8842
1679.8842
1679.8842
1896.7908
-0.65
0
40
0.099
1
0.11
0
48
0.027
1
-0.59
0
47
0.019
1
-0.52
0
(25)
3
1
-0.24
0
(46) 0.019
1
-0.16
0
(27)
1.8
1
-0.26
1
28
0.4
1
Peptide
K.VSPAVFVSR.E
K.VAALGSDPHISMK.V
R.EGQEQEGEEMAEYRGR.V
12/9/2009
Page 5 of 15
gi|3183510
Mass: 59239
Score: 163
Queries matched: 7
gi|12647311
Mass: 32265
Score: 163
Queries matched: 7
Mass: 59225
Score: 163
Queries matched: 7
gi|27806233
13.
gi|157164220
Mass: 18025
Score: 158
Queries matched: 3
50S ribosomal protein L10 [Campylobacter concisus 13826]
emPAI: 0.41
Query
67
173
284
14.
Observed
479.2902
608.2799
797.9197
Mr(expt)
956.5658
1214.5452
1593.8248
Mr(calc)
956.5654
1214.5455
1593.8250
gi|28317
Mass: 59492
Score: 155
0.42
0
53
0.009
1
-0.22
0
36
0.13
1
-0.09
1
70 0.00013
1
Queries matched: 4
Peptide
K.NTLANIALK.N
K.FEESNSDLFK.I
K.TAYIDGEVASVDKVK.A
emPAI: 0.18
Query
104
196
221
309
Observed
516.3027
631.8024
691.3277
854.3903
Mr(expt)
1030.5908
1261.5902
1380.6408
1706.7660
Mr(calc)
1030.5910
1261.5899
1380.6408
1706.7649
-0.17
0
34
0.22
1
0.31
0
42
0.065
1
0.01
0
46
0.03
1
0.67
0
33
0.31
1
Peptide
R.VLDELTLTK.A
R.SLLEGEGSSGGGGR.G
R.ALEESNYELEGK.I
gi|21961605
Mass: 58792
Score: 155
Queries matched: 4
gi|114667507
Mass: 100282
Score: 155
Queries matched: 4
gi|114667509
Mass: 62761
Score: 155
Queries matched: 4
gi|114667511
Mass: 58244
Score: 155
Queries matched: 4
gi|114667513
Mass: 56692
Score: 155
Queries matched: 4
Mass: 63308
Score: 155
Queries matched: 4
gi|119581085
gi|149723838
Mass: 61580
Score: 155
Queries matched: 4
gi|195972866
Mass: 58766
Score: 155
Queries matched: 4
Mass: 58818
Score: 155
Queries matched: 4
gi|251757513
15.
gi|6320157
Mass: 19051
Score: 151
Queries matched: 12
emPAI: 0.18
Query
2
181
182
183
184
185
186
219
Observed
353.7157
617.8554
412.2394
617.8557
617.8557
617.8560
617.8560
681.9034
Mr(expt)
705.4168
1233.6962
1233.6964
1233.6968
1233.6968
1233.6974
1233.6974
1361.7922
Mr(calc)
705.4174
1233.6969
1233.6969
1233.6969
1233.6969
1233.6969
1233.6969
1361.7918
-0.72
0
30
0.99
4
-0.52
0
(39) 0.062
1
-0.42
0
(21)
4.5
1
-0.03
0
46
0.016
1
-0.03
0
(40) 0.058
1
0.46
0
(42) 0.034
1
0.46
0
(32)
0.37
1
0.30
1
41
0.018
1
Peptide
K.YVVVAR.A
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.KIVEIPGEVPPGK.Y
353
355
356
357
961.5585
641.3749
961.5588
641.3754
1921.1024
1921.1029
1921.1030
1921.1044
1921.1037
1921.1037
1921.1037
1921.1037
-0.64
-0.42
-0.33
0.36
K.IVEIPGEVPPGKYVVVAR.A
1
1
1
1
(27)
(29)
(21)
34
0.27
0.17
0.93
0.067
1
1
1
1
gi|151941952
Mass: 19021
Score: 151
Queries matched: 12
gi|190405057
Mass: 19007
Score: 151
Queries matched: 12
16.
gi|157165665
Mass: 19221
Score: 145
Queries matched: 3
emPAI: 0.62
outer-membrane lipoprotein carrier protein [Campylobacter concisus 13826]
Query
301
312
Observed
837.4047
861.4227
Mr(expt)
1672.7948
1720.8308
Mr(calc)
1672.7944
1720.8308
0.24
0
55 0.0026
1
0.03
0
50
0.012
1
Peptide
K.SLQSDFTQTVFSEGK.S
K.SDNTALWIYESPTPK.R
12/9/2009
332
17.
893.9647
1785.9148
1785.9149
-0.02
0
40
0.11
gi|2232299
Mass: 47885
Score: 145
Queries matched: 5
1
Page 6 of 15
K.ETLTPVIPQGYDIVNQ.-
emPAI: 0.07
Query
122
127
197
331
393
Observed
547.2811
554.2888
633.8091
892.4928
1110.5790
Mr(expt)
1092.5476
1106.5630
1265.6036
1782.9710
2219.1434
Mr(calc)
1092.5485
1106.5641
1265.6040
1782.9702
2219.1434
-0.79
0
(27)
2.7
1
-0.99
0
30
1.4
2
-0.30
0
37
0.27
1
0.47
0
28
1.1
1
0.02
0
50 0.0095
1
Peptide
K.ALETHTYFER.H
gi|3834667
Mass: 48183
Score: 145
Queries matched: 5
gi|24496448
Mass: 65621
Score: 145
Queries matched: 5
immunoglobulin M heavy chain secretory form [Bos taurus]
gi|33413902
Mass: 49106
Score: 145
Queries matched: 5
immunoglobulin mu heavy chain constant region; IgM heavy chain constant region [Bos taurus]
18.
gi|158604950
Mass: 13498
Score: 128
Queries matched: 2
emPAI: 0.57
Query
213
280
19.
Observed
660.8353
794.4047
Mr(expt)
1319.6560
1586.7948
gi|7331218
Mass: 65978
Mr(calc)
1319.6568
1586.7940
ppm Miss Score
Expect Rank
-0.60
0
86 3.2e-006
1
0.52
0
42
0.072
1
Score: 124
Peptide
R.STAAAAAIDSSDLK.I
R.TPSEWQETGIIAGAK.T
Queries matched: 4
Query
72
106
229
243
Observed
487.2695
517.2611
697.3694
738.3973
Mr(expt)
972.5244
1032.5076
1392.7242
1474.7800
Mr(calc)
972.5240
1032.5087
1392.7249
1474.7780
0.49
0
23
9.9
1
-1.05
0
39
0.13
1
-0.45
1
34
0.39
1
1.40
0
28
2
1
Peptide
K.IEISELNR.V
R.TLLEGEESR.M
R.TNAENEFVTIKK.D
R.FLEQQNQVLQTK.W
Mass: 66027
Score: 124
Queries matched: 4
gi|11935049
gi|109096823
Mass: 65170
Score: 124
Queries matched: 4
gi|109096825
Mass: 64332
Score: 124
Queries matched: 4
gi|109096827
Mass: 62980
Score: 124
Queries matched: 4
gi|109096829
Mass: 61838
Score: 124
Queries matched: 4
Mass: 61566
Score: 124
Queries matched: 4
gi|109096833
Mass: 58846
Score: 124
Queries matched: 4
gi|109096835
gi|119395750
Mass: 65999
Score: 124
Queries matched: 4
gi|160961491
Mass: 65450
Score: 124
Queries matched: 4
20.
gi|3914346
Mass: 82383
Score: 124
Queries matched: 6
emPAI: 0.04
Query
66
73
165
166
167
171
21.
Observed
478.7737
488.2789
398.2151
596.8197
596.8198
604.8077
Mr(expt)
955.5328
974.5432
1191.6235
1191.6248
1191.6250
1207.6008
Mr(calc)
955.5338
974.5437
1191.6248
1191.6248
1191.6248
1207.6020
-1.04
0
22
4.2
1
-0.43
0
24
6.7
1
-1.08
1
(26)
3.4
1
0.08
1
47
0.027
1
0.25
1
(28)
2.4
1
-0.92
0
30
1.1
1
gi|57164373
Mass: 69143
Score: 123
Peptide
K.VVQGEPSLK.V
R.WISTVELK.V
R.GLIKEQYEGR.L
R.GLIKEQYEGR.L
R.GLIKEQYEGR.L
Queries matched: 4
Query
9
Observed
395.2395
Mr(expt)
788.4644
Mr(calc)
788.4644
0.10
0
25
2.2
1
Peptide
K.IVTDLTK.V
12/9/2009
93
141
210
507.8131
571.8608
653.3609
1013.6116
1141.7070
1304.7072
1013.6121
1141.7070
1304.7088
-0.42
0.01
-1.21
Mass: 66270
Score: 123
gi|193085052
22.
gi|181402
Mass: 65825
Score: 114
0
1
0
41
27
31
0.042
0.41
0.85
1
1
1
Page 7 of 15
K.QTALVELLK.H
K.KQTALVELLK.H
K.HLVDEPQNLIK.K
Queries matched: 4
Queries matched: 3
emPAI: 0.05
Query
72
192
243
Observed
487.2695
627.8072
738.3973
Mr(expt)
972.5244
1253.5998
1474.7800
Mr(calc)
972.5240
1253.6001
1474.7780
0.49
0
23
9.9
1
-0.16
0
63 0.0005
1
1.40
0
28
2
1
Peptide
K.IEISELNR.V
R.GFSSGSAVVSGGSR.R
R.FLEQQNQVLQTK.W
gi|47132620
Mass: 65393
Score: 114
Queries matched: 3
gi|109096839
Mass: 65462
Score: 114
Queries matched: 3
PREDICTED: similar to keratin 2a [Macaca mulatta]
gi|148727309
Mass: 65944
Score: 114
Queries matched: 3
23.
gi|157165213
Mass: 19144
Score: 105
Queries matched: 4
24.
Query
64
Observed
475.7508
Mr(expt)
949.4870
Mr(calc)
949.4869
116
117
263
360.2010
539.7979
755.8916
1077.5812
1077.5812
1509.7686
1077.5818
1077.5818
1509.7675
0.20
0
44
0.086
1
-0.60
-0.53
0.77
1
1
1
31
(24)
30
1.1
5.3
0.84
1
2
1
Peptide
K.ADAIIEYR.K
K.ADAIIEYRK.A
K.ADAIIEYRK.A
K.TFDNLKGEISTTGK.S
gi|122614
Mass: 15998
Score: 77
Queries matched: 4
emPAI: 0.47
Query
201
376
378
379
Observed
637.8663
1000.4650
672.6437
1008.4630
Mr(expt)
1273.7180
1998.9154
2014.9093
2014.9114
Mr(calc)
1273.7183
1998.9146
2014.9095
2014.9095
-0.19
0
34
0.29
1
0.43
0
43
0.038
1
-0.11
0
(29)
0.87
1
0.97
0
(22)
3.9
1
Peptide
R.LLVVYPWTQR.F
R.FFDSFGDLSNPGAVMGNPK.V
gi|230480
Mass: 15970
Score: 77
Queries matched: 4
Mass: 16129
Score: 77
Queries matched: 4
gi|255683515
25.
gi|27806789
Mass: 15717
Score: 74
Queries matched: 2
emPAI: 0.22
Query
49
232
Observed
450.2507
704.8226
Mr(expt)
898.4868
1407.6306
Mr(calc)
898.4872
1407.6306
-0.42
0
38
0.13
1
0.00
0
36
0.13
1
Peptide
R.GSPAANVGVK.V
K.AADETWEPFASGK.T
Mass: 15761
Score: 74
Queries matched: 2
gi|57526651
26.
gi|157165156
Mass: 18459
Score: 72
Queries matched: 2
thiol peroxidase (scavengase P20) [Campylobacter concisus 13826]
emPAI: 0.18
Query
205
273
27.
Observed
641.3160
775.4063
Mr(expt)
1280.6174
1548.7980
Mr(calc)
1280.6183
1548.7970
-0.68
0
48
0.025
1
0.68
0
25
4.2
1
gi|157164172
Mass: 17412
Score: 69
Queries matched: 1
flavodoxin FldA [Campylobacter concisus 13826]
Peptide
R.FCTTEGIANLR.V + Carbamidomethyl (C)
K.LSIISNDLPFAMGR.F + Oxidation (M)
emPAI: 0.19
Query
Observed
Mr(expt)
Mr(calc)
ppm
Peptide
12/9/2009
375
28.
987.4581
1972.9016
1972.9015
0.10
0
69
0.0001
1
Page 8 of 15
K.VVGEVSTDGYTFDGSDAVR.N
gi|57169062
Score: 68
Queries matched: 1
ribosomal protein L13 [Campylobacter coli RM2228]
Query
137
Observed
564.8526
Mr(expt)
1127.6906
Mr(calc)
1127.6914
ppm Miss Score
Expect Rank
-0.66
0
68 5.1e-005
1
Peptide
R.LLTEVATILR.G
gi|86152993
Score: 68
Queries matched: 1
gi|121612364
Score: 68
Queries matched: 1
gi|153951839
Score: 68
Queries matched: 1
gi|154175256
Score: 68
Queries matched: 1
Score: 68
Queries matched: 1
gi|157165604
gi|222823419
Score: 68
Queries matched: 1
29.
gi|157165390
Mass: 16384
Score: 67
Queries matched: 1
emPAI: 0.21
Query
150
30.
Observed
576.3427
Mr(expt)
1150.6708
Mr(calc)
1150.6710
gi|504
Mass: 75134
Score: 67
ppm Miss Score
Expect Rank
-0.11
0
67 5.2e-005
1
Queries matched: 4
Peptide
K.APPALLAELTR.N
emPAI: 0.04
Query
339
340
341
342
Observed
914.4407
914.4412
914.4418
914.4434
Mr(expt)
1826.8668
1826.8678
1826.8690
1826.8722
Mr(calc)
1826.8686
1826.8686
1826.8686
1826.8686
-0.98
0
(25)
3.4
1
-0.43
0
(35)
0.37
1
0.22
0
67 0.00025
1
1.98
0
(51)
0.01
1
Peptide
gi|506
Mass: 78070
Score: 67
Queries matched: 4
gi|163285
Mass: 77873
Score: 67
Queries matched: 4
lactoferrin
Mass: 78117
Score: 67
Queries matched: 4
gi|408928
lactoferrin
gi|9650974
Mass: 78025
Score: 67
Queries matched: 4
gi|22795592
Mass: 38836
Score: 67
Queries matched: 4
Mass: 78006
Score: 67
Queries matched: 4
gi|30794292
Mass: 37957
Score: 67
Queries matched: 4
gi|34810001
gi|46015771
Mass: 36554
Score: 67
Queries matched: 4
Mass: 37586
Score: 67
Queries matched: 4
gi|83754256
gi|88702501
Mass: 78004
Score: 67
Queries matched: 4
gi|146157606
Mass: 78129
Score: 67
Queries matched: 4
gi|146387335
Mass: 37439
Score: 67
Queries matched: 4
gi|157830374
Mass: 76095
Score: 67
Queries matched: 4
gi|170672383
Mass: 77975
Score: 67
Queries matched: 4
gi|170672385
Mass: 78006
Score: 67
Queries matched: 4
gi|193299659
Mass: 78006
Score: 67
Queries matched: 4
gi|255762013
Mass: 76226
Score: 67
Queries matched: 4
31.
gi|83764016
Mass: 187175
Score: 65
Queries matched: 1
At 1.5a Resolution
emPAI: 0.02
Query
218
Observed
681.3369
Mr(expt)
1360.6592
Mr(calc)
1360.6583
0.72
1
65 0.00029
1
Peptide
K.SGSDEVQVKQER.K
12/9/2009
Page 9 of 15
gi|99028969
Mass: 187112
Score: 65
Queries matched: 1
gi|124056491
Mass: 187135
Score: 65
Queries matched: 1
32.
gi|158604982
Mass: 16988
Score: 62
Queries matched: 1
emPAI: 0.20
phosphoribosylaminoimidazole carboxylase, catalytic subunit [Campylobacter concisus 13826]
Query
187
33.
Observed
621.2983
Mr(expt)
1240.5820
Mr(calc)
1240.5823
-0.20
0
62 0.00052
1
Peptide
K.SDYEIVSETAK.T
gi|157164111
Mass: 16254
Score: 55
Queries matched: 1
emPAI: 0.21
Query
258
34.
Observed
751.3621
Mr(expt)
1500.7096
Mr(calc)
1500.7096
0.00
0
55 0.0027
1
gi|122064583
Mass: 15882
Score: 52
RecName: Full=Superoxide dismutase [Cu-Zn]
Peptide
K.DGSQEVFATYIGSK.K
Queries matched: 2
Query
56
103
35.
Observed
466.2945
516.2805
Mr(expt)
930.5744
1030.5464
Mr(calc)
930.5750
1030.5481
gi|538275
Mass: 22697
Score: 51
-0.59
0
23
1.6
2
-1.63
0
29
1.5
1
Queries matched: 1
Peptide
K.TVLVTGTIK.G
R.LACGVIGITQ.- + Carbamidomethyl (C)
emPAI: 0.15
Query
133
Observed
561.3135
Mr(expt)
1120.6124
Mr(calc)
1120.6128
-0.33
0
51 0.0071
1
Peptide
K.DTQSIIFLGK.V
gi|1245695
Mass: 28553
Score: 51
Queries matched: 1
Mass: 9788
Score: 51
Queries matched: 1
gi|1245697
gi|31340900
Mass: 46175
Score: 51
Queries matched: 1
gi|84000377
Mass: 46297
Score: 51
Queries matched: 1
gi|121531628
Mass: 46282
Score: 51
Queries matched: 1
gi|126165236
Mass: 46368
Score: 51
Queries matched: 1
gi|126165264
Mass: 46888
Score: 51
Queries matched: 1
Mass: 46935
Score: 51
Queries matched: 1
gi|151554250
gi|160332365
Mass: 46208
Score: 51
Queries matched: 1
gi|194038345
Mass: 38368
Score: 51
Queries matched: 1
gi|194038353
Mass: 47192
Score: 51
Queries matched: 1
gi|226373737
Mass: 46208
Score: 51
Queries matched: 1
gi|226373739
Mass: 46361
Score: 51
Queries matched: 1
36.
gi|115495209
Mass: 59194
Score: 51
Queries matched: 2
zymogen granule membrane glycoprotein 2 [Bos taurus]
Query
12
161
37.
Observed
403.7109
587.7978
Mr(expt)
805.4072
1173.5810
Mr(calc)
805.4083
1173.5812
-1.28
0
21
8.1
2
-0.12
0
30
1.2
1
Peptide
R.FVGDGGVR.M
R.LEGTPQCSLR.Y + Acrylamide (C)
gi|123506
Mass: 8353
Score: 50
Queries matched: 1
emPAI: 0.42
Query
75
Observed
492.3033
Mr(expt)
982.5920
Mr(calc)
982.5923
-0.29
0
50 0.0033
1
Peptide
R.ALGILNLNR.N
12/9/2009
Page 10 of 15
Mass: 12474
Score: 50
Queries matched: 1
gi|5019730
gi|76782253
Mass: 12343
Score: 50
Queries matched: 1
38.
gi|67408
Mass: 16156
Score: 50
Queries matched: 1
emPAI: 0.21
lysozyme (EC 3.2.1.17) c precursor [validated] - ring-necked pheasant (tentative sequence)
Query
323
Observed
877.4213
Mr(expt)
1752.8280
Mr(calc)
1752.8278
0.11
0
50 0.0088
1
Peptide
R.NTDGSTDYGILQINSR.W
gi|126592
Mass: 16352
Score: 50
Queries matched: 1
RecName: Full=Lysozyme C-1; AltName: Full=1,4-beta-N-acetylmuramidase C; Flags: Precursor
gi|126608
Mass: 16228
Score: 50
Queries matched: 1
RecName: Full=Lysozyme C; AltName: Full=1,4-beta-N-acetylmuramidase C; AltName: Full=Allergen Gal d IV; AltName: Allergen=Gal d 4; Flags: Precursor
gi|126609
Mass: 14302
Score: 50
Queries matched: 1
RecName: Full=Lysozyme C; AltName: Full=1,4-beta-N-acetylmuramidase C
gi|126618
Mass: 14291
Score: 50
Queries matched: 1
RecName: Full=Lysozyme C; AltName: Full=1,4-beta-N-acetylmuramidase
gi|126619
Mass: 16124
Score: 50
Queries matched: 1
RecName: Full=Lysozyme C; AltName: Full=1,4-beta-N-acetylmuramidase C; Flags: Precursor
gi|126624
Mass: 14413
Score: 50
Queries matched: 1
Mass: 14403
Score: 50
Queries matched: 1
gi|126655
Mass: 14305
Score: 50
Queries matched: 1
gi|229157
lysozyme
Mass: 14304
Score: 50
Queries matched: 1
gi|229916
Chain Y, Crystallographic Refinement Of The Three-Dimensional Structure Of The Fab D1.3-Lysozyme Complex At 2.5- Angstroms Resolution
gi|350714
Mass: 14469
Score: 50
Queries matched: 1
lysozyme DL1
gi|350715
Mass: 14528
Score: 50
Queries matched: 1
lysozyme DL3
Mass: 14304
Score: 50
Queries matched: 1
gi|442943
Chain A, The Three-Dimensional Structure Of Pheasant And Guinea-Fowl Egg Lysozymes
gi|494223
Mass: 14248
Score: 50
Queries matched: 1
Chain A, Three-Dimensional Structure Of A Heteroclitic Antigen- Antibody Cross-Reaction Complex
gi|539969
Mass: 3162
Score: 50
Queries matched: 1
lysozyme homolog AT-2, bone - rat (fragments)
Mass: 16155
Score: 50
Queries matched: 1
gi|547878
Mass: 14276
Score: 50
Queries matched: 1
gi|576405
Chain Y, Structure Of An Antibody-Lysozyme Complex: Effect Of A Conservative Mutation
gi|742827
Mass: 14293
Score: 50
Queries matched: 1
lysozyme
gi|1346475
Mass: 14305
Score: 50
Queries matched: 1
gi|2851416
Mass: 16267
Score: 50
Queries matched: 1
gi|3212285
Mass: 14260
Score: 50
Queries matched: 1
Chain C, Hen Egg White Lysozyme, D18a Mutant, In Complex With Mouse Monoclonal Antibody D1.3
gi|8928169
Mass: 14277
Score: 50
Queries matched: 1
RecName: Full=Lysozyme C; AltName: Full=1,4-beta-N-acetylmuramidase C; AltName: Full=CPL
gi|10120552
Mass: 14318
Score: 50
Queries matched: 1
Chain A, Hen Egg White Lysozyme Mutant With Alanine Substituted For Glycine
gi|10120553
Mass: 14318
Score: 50
Queries matched: 1
gi|10120554
Mass: 14318
Score: 50
Queries matched: 1
gi|10120555
Mass: 14318
Score: 50
Queries matched: 1
gi|11514088
Mass: 14200
Score: 50
Queries matched: 1
Chain X, Crystal Structure Of Phage Library-Derived Single-Chain Fv Fragment 1f9 In Complex With Turkey Egg-White Lysozyme
Mass: 14320
Score: 50
Queries matched: 1
gi|14278671
Chain A, Stabilization Of Hen Egg White Lysozyme By A Cavity-Filling Mutation
gi|14278672
Mass: 14286
Score: 50
Queries matched: 1
Chain A, Stabilization Of Hen Egg White Lysozyme By A Cavity-Filling Mutation
gi|15826593
Mass: 14303
Score: 50
Queries matched: 1
Chain A, Covalent Glycosyl-Enzyme Intermediate Of Hen Egg White Lysozyme
gi|15988033
Mass: 14322
Score: 50
Queries matched: 1
Chain A, Im Mutant Of Lysozyme
gi|15988035
Mass: 14322
Score: 50
Queries matched: 1
Chain A, Im Mutant Of Lysozyme
gi|29726605
Mass: 14247
Score: 50
Queries matched: 1
Chain C, Crystal Structure Of Hyhel-63 Complexed With Hel Mutant K96a
gi|29726608
Mass: 14247
Score: 50
Queries matched: 1
Chain C, Crystal Structure Of Hyhel-63 Complexed With Hel Mutant K97a
12/9/2009
Page 11 of 15
gi|45384212
Mass: 16229
Score: 50
Queries matched: 1
lysozyme [Gallus gallus]
gi|47117003
Mass: 14314
Score: 50
Queries matched: 1
gi|47117009
Mass: 14229
Score: 50
Queries matched: 1
gi|47169429
Mass: 14302
Score: 50
Queries matched: 1
Chain A, Triclinic Hen Lysozyme Crystallized At 313k From A D2o Solution
Mass: 14220
Score: 50
Queries matched: 1
gi|48428233
Mass: 14218
Score: 50
Queries matched: 1
gi|157829560
Chain A, Structural Consequences Of Reductive Methylation Of Lysine Residues In Hen Egg White Lysozyme: An X-Ray Analysis At 1.8 Angstroms Resolution
gi|157830068
Mass: 14354
Score: 50
Queries matched: 1
Chain A, Hen Egg White Lysozyme With A Succinimide Residue
gi|157830069
Mass: 14300
Score: 50
Queries matched: 1
Chain A, Hen Egg White Lysozyme With A Isoaspartate Residue
gi|157831313
Mass: 14318
Score: 50
Queries matched: 1
Chain A, Structural And Thermodynamic Analysis Of Compensating Mutations Within The Core Of Chicken Egg White Lysozyme
gi|157831317
Mass: 14304
Score: 50
Queries matched: 1
gi|157831318
Mass: 14290
Score: 50
Queries matched: 1
gi|157831653
Mass: 14305
Score: 50
Queries matched: 1
Chain A, Analysis Of The Stabilization Of Hen Lysozyme With The Helix Dipole And Charged Side Chains
gi|157831654
Mass: 14304
Score: 50
Queries matched: 1
Mass: 14303
Score: 50
Queries matched: 1
gi|157831655
gi|157831881
Mass: 15862
Score: 50
Queries matched: 1
Chain A, Three-Dimensional Structure Of The Platelet Integrin Recognition Segment Of The Fibrinogen Gamma Chain Obtained By Carrier Protein-Driven Cr
gi|157831884
Mass: 14287
Score: 50
Queries matched: 1
Chain A, Thermal Stability Determinants Of Chicken Egg-White Lysozyme Core Mutants: Hydrophobicity, Packing Volume And Conserved Buried Water Molecul
gi|157831918
Mass: 14281
Score: 50
Queries matched: 1
Chain A, Dissection Of Protein-Carbohydrate Interactions In Mutant Hen Egg-White Lysozyme Complexes And Their Hydrolytic Activity
gi|157831920
Mass: 14265
Score: 50
Queries matched: 1
Chain A, Dissection Of Protein-Carbohydrate Interactions In Mutant Hen Egg-White Lysozyme Complexes And Their Hydrolytic Activity
Mass: 14011
Score: 50
Queries matched: 1
gi|157834075
gi|157834077
Mass: 14238
Score: 50
Queries matched: 1
Mass: 14314
Score: 50
Queries matched: 1
gi|157834078
gi|157834079
Mass: 14224
Score: 50
Queries matched: 1
gi|157834080
Mass: 14266
Score: 50
Queries matched: 1
Mass: 14357
Score: 50
Queries matched: 1
gi|157835197
Chain A, Lysozyme (E.C.3.2.1.17) (Japanese Quail)
gi|157878404
Mass: 14287
Score: 50
Queries matched: 1
Chain A, Hydrogen And Hydration Of Hen Egg-White Lysozyme Determined By Neutron Diffraction
gi|209870537
Mass: 14928
Score: 50
Queries matched: 1
Chain A, Effect Of Ariginine On Lysozyme
Query
178
244
208
96
43
234
266
94
298
46
164
36
97
127
118
2
297
29
42
81
95
56
34
28
159
Observed
613.7897
738.8691
644.8588
508.7721
435.7740
710.8641
505.9110
508.2985
827.4570
439.7524
596.3184
428.7660
510.2691
554.2888
540.2955
353.7157
551.9736
421.7707
435.7738
497.2574
508.7479
466.2945
427.2391
421.7584
585.2886
Mr(expt)
1225.5648
1475.7236
1287.7030
1015.5296
869.5334
1419.7136
1514.7112
1014.5824
1652.8994
877.4902
1190.6222
855.5174
1018.5236
1106.5630
1078.5764
705.4168
1652.8990
841.5268
869.5330
992.5002
1015.4812
930.5744
852.4636
841.5022
1168.5626
Mr(calc)
1225.5649
1475.7216
1287.7034
1015.5298
869.5334
1419.7146
1514.7113
1014.5822
1652.8985
877.4909
1190.6217
855.5178
1018.5229
1106.5642
1078.5771
705.4174
1652.8985
841.5273
869.5334
992.5001
1015.4822
930.5750
852.4640
841.5022
1168.5625
ppm Miss Score
-0.03
0
46
1.36
0
44
-0.27
1
43
-0.15
0
42
0.00
0
42
-0.70
0
42
-0.12
0
42
0.25
0
41
0.57
0
40
-0.78
0
40
0.50
0
38
-0.42
0
37
0.69
1
34
-1.01
0
33
-0.59
0
33
-0.72
0
33
0.28
0
32
-0.53
0
31
-0.46
0
31
0.16
0
31
-0.92
0
30
-0.56
0
29
-0.38
0
29
0.12
0
28
0.10
0
28
Expect Rank
0.022
1
0.047
1
0.049
1
0.089
1
0.075
1
0.083
1
0.052
1
0.12
1
0.06
1
0.13
1
0.18
1
0.28
1
0.78
1
0.6
1
0.45
1
0.58
1
0.4
1
0.43
1
0.97
1
0.9
1
0.7
1
0.41
1
0.44
1
2.3
1
1.8
1
Peptide
MDTGVGNYELK
VNDAVTTDGVQTTR
NLENTVKETIK
SVIAEENVR
VVSLSIPR
YVNWIQQTIAAN
VGAHAGDYGAEALER
VTGIITQGAR
VGAPLVEGATVVGEVEK
IFGVVDTK
LATVVTPR
KMKPGDATR + Oxidation (M)
CLSVVSSDVK + Acrylamide (C)
HIDELSLPR
YVVGIR
VGAPLVEGATVVGEVEK
LTIPAVTK
VVSLSIPR
EPGIYGVMK
TPELEENGK
SILVATLSK
LAPHMIR + Oxidation (M)
GITLSVRP
IQHQDWTGGK
12/9/2009
Page 12 of 15
21
92
61
299
59
277
17
138
53
18
417.7285
507.7185
475.2729
834.9258
471.2232
783.8735
410.7244
565.3323
455.2545
410.7267
833.4424
1013.4224
948.5312
1667.8370
940.4318
1565.7324
819.4342
1128.6500
908.4944
819.4388
833.4429
1013.4236
948.5280
1667.8366
940.4324
1565.7283
819.4346
1128.6503
908.4967
819.4391
-0.54
-1.18
3.41
0.26
-0.59
2.65
-0.49
-0.18
-2.52
-0.37
1
0
0
0
0
1
0
1
0
0
28
28
27
27
27
27
27
26
26
26
4.1
0.35
2.3
2.1
1.6
1.9
2.6
2.8
2.2
1.1
1
1
1
1
1
1
1
1
1
1
LKSLDCR
ADFSGMSSGR
ILVYEGQK
IIPSAEDPSQDIVER
GECYATIGK
MIGAMGLK
IKQGDISQIK
EGVSVPPPK
AIWQFR
117
225
12
115
240
88
45
202
177
14
15
152
89
275
539.7979
692.8684
403.7109
534.2764
721.3693
503.7405
437.7531
639.3042
612.3717
409.7166
409.7238
579.2989
503.7637
781.9185
1077.5812
1383.7222
805.4072
1066.5382
1440.7240
1005.4664
873.4916
1276.5938
1222.7288
817.4186
817.4330
1156.5832
1005.5128
1561.8224
1077.5818
1383.7227
805.4082
1066.5342
1440.7249
1005.4702
873.4920
1276.5904
1222.7285
817.4181
817.4334
1156.5836
1005.5165
1561.8212
-0.54
-0.35
-1.24
3.80
-0.58
-3.73
-0.39
2.72
0.28
0.64
-0.44
-0.35
-3.60
0.78
0
0
1
1
0
0
0
0
0
0
0
0
1
1
25
25
25
24
24
24
24
23
23
23
23
23
22
22
4
3.2
3.5
4.6
5.1
3.7
11
4.8
1.7
8.9
3.1
4.9
11
6.9
1
1
1
1
1
1
1
1
1
1
1
1
1
1
GEALLEPPPR
FFVAPFPEVFGK
FARQGEV
RCFVGTASR + Acrylamide (C)
TYGAELASVDFLR
VSDWGCIR + Acrylamide (C)
TVLVNGGSK
VEVNLAAIPLGK
EDNSIIK
VDVNPFK
QGVDADINGLR
MISDKVDAK
NISNNQTKFELVR
231
23
276
41
288
126
111
193
101
8
30
22
290
114
76
99
239
139
212
255
321
27
354
406
352
310
327
238
701.8188
418.7219
783.8734
434.2415
545.6295
552.2544
526.2639
628.3116
512.2405
393.7396
424.7531
417.7321
817.9414
533.2639
493.7797
510.7712
716.8761
565.7697
660.3185
750.3656
584.2764
421.7583
641.3749
583.2827
961.5583
857.4078
886.4160
713.8464
1401.6230
835.4292
1565.7322
866.4684
1633.8667
1102.4942
1050.5132
1254.6086
1022.4664
785.4646
847.4916
833.4496
1633.8682
1064.5132
985.5448
1019.5278
1431.7376
1129.5248
1318.6224
1498.7166
1749.8074
841.5020
1921.1029
2329.1017
1921.1020
1712.8010
1770.8174
1425.6782
1401.6275
835.4262
1565.7330
866.4684
1633.8610
1102.4965
1050.5128
1254.6100
1022.4703
785.4647
847.4916
833.4494
1633.8709
1064.5138
985.5444
1019.5301
1431.7358
1129.5291
1318.6234
1498.7164
1749.8090
841.5022
1921.1037
2329.1056
1921.1037
1712.8014
1770.8168
1425.6776
-3.17
3.64
-0.48
0.06
3.47
-2.01
0.45
-1.10
-3.73
-0.04
0.08
0.28
-1.63
-0.56
0.47
-2.20
1.32
-3.79
-0.72
0.13
-0.95
-0.12
-0.42
-1.66
-0.85
-0.23
0.36
0.46
0
0
1
1
1
0
1
1
0
0
0
0
1
0
0
0
0
0
0
0
1
0
1
1
1
1
0
0
22
22
22
21
21
21
21
21
21
21
21
21
21
21
21
20
20
20
20
20
20
20
20
20
19
19
19
19
3.7
7.1
6.4
4.8
7.9
3.4
12
6.3
11
13
12
11
6.4
13
10
12
16
6.4
10
9.8
8.9
17
1.4
13
1.6
11
10
14
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
DTWFMVEEFAK
MFGPLQK + Oxidation (M)
VEMAGLARMYHEK + 2 Oxidation (M)
MAFGGLKK + Oxidation (M)
VCDSLLGRITFSPR + Acrylamide (C)
DELLNCDPK + Carbamidomethyl (C)
KTMDALNSR + Oxidation (M)
MGELICKYNGK
TSMGDQIGSK
NDIAIIK
KPTFSLR
ESAISSLK
MGDSTVLDATVRILK + Oxidation (M)
EDAVEFTVR
LVDLIEER
SLHHNVWK
VLEGNEQFINAAK
YYVEEVSNK
WFEPFTTTYK
VGGHAGEFGAEALER
IMENQSEELEEKQK + Oxidation (M)
VATVSLPR
IVEIPGEVPPGKYVVVAR
QARACWGDNMTQSVLIHGQAK + Oxidation (M)
IVEIPGEVPPGKYVVVAR
CFNCGKPLEFKDGK + Acrylamide (C); Carbamidomethyl (C)
VILCNAITGEMEDYK + Carbamidomethyl (C); Oxidation (M)
DFTPELQASYQK
86
154
235
399
131
237
74
367
260
241
102
361
188
100
500.2483
581.7905
710.8751
772.7022
560.7438
713.3224
490.2841
968.0127
755.3726
483.9367
513.2398
484.5090
623.2901
511.2328
998.4820
1161.5664
1419.7356
2315.0848
1119.4730
1424.6302
978.5536
1934.0108
1508.7306
1448.7883
1024.4650
1934.0069
1244.5656
1020.4510
998.4781
1161.5634
1419.7357
2315.0866
1119.4729
1424.6276
978.5538
1934.0077
1508.7300
1448.7888
1024.4616
1934.0044
1244.5646
1020.4546
3.95
2.58
-0.07
-0.77
0.16
1.88
-0.20
1.61
0.45
-0.38
3.34
1.29
0.83
-3.49
0
1
1
0
0
1
0
1
0
1
1
1
0
0
19
18
18
18
18
17
17
17
17
17
17
17
17
17
14
23
19
16
5.1
12
20
18
21
13
23
19
11
17
1
1
1
1
1
1
1
1
1
1
1
1
1
1
ANVNNGDAPK
CVPKLDGMAR + Carbamidomethyl (C); Oxidation (M)
INYELKNLDNGK
GLPAGLHGFHVHQNADCGATEK + Carbamidomethyl (C)
ENMYLYCK + Carbamidomethyl (C)
DDVNVEKMMQGK + 2 Oxidation (M)
FAISVPFAK
MVSVCALQNLVVSSRANK + Oxidation (M)
NGFGEGEWIPYLK
VVAGVANALAHKYH
RMMVGCGGAK + Oxidation (M)
TGDLGYLVDGQIVICGRR
HHANEAEAGPGR
GEGEGAGMLGK + Oxidation (M)
216
16
62
153
343
10
415
329
248
674.3350
410.7244
475.7145
580.7902
922.4322
396.2182
905.0770
887.9521
747.3699
1346.6554
819.4342
949.4144
1159.5658
1842.8498
790.4218
2712.2092
1773.8896
1492.7252
1346.6541
819.4347
949.4109
1159.5622
1842.8492
790.4198
2712.2151
1773.8897
1492.7270
1.04
-0.51
3.68
3.16
0.37
2.57
-2.17
-0.03
-1.16
0
0
0
0
0
0
0
0
1
17
17
17
17
17
16
16
16
16
16
24
17
23
20
12
13
34
29
1
1
1
1
1
1
1
1
1
MEVPQWVDTVK + Oxidation (M)
MLCAVGVK
GANTAFTPPER
MIGIVYGSSMGNTEDAAK
TAHHAVR
FEQASESEPTTVSYEIAGNSPNAER
INTVQEAIDFVAANAAQ
SQYEQLAEQNRK
12/9/2009
Page 13 of 15
48
294
214
160
1
143
247
40
65
407
366
448.7176
818.3919
442.5662
585.8549
353.7156
573.3187
745.4220
432.7494
478.2533
777.3746
968.0126
895.4206
1634.7692
1324.6768
1169.6952
705.4166
1144.6228
1488.8294
863.4842
954.4920
2329.1020
1934.0106
895.4222
1634.7675
1324.6809
1169.6954
705.4174
1144.6240
1488.8260
863.4865
954.4957
2329.1022
1934.0109
-1.71
1.05
-3.12
-0.16
-1.01
-1.04
2.32
-2.60
-3.79
-0.10
-0.13
0
1
0
0
0
0
1
0
0
0
0
16
16
16
16
16
16
15
15
15
15
15
29
28
29
7.1
28
44
18
50
33
37
32
1
1
1
1
1
1
1
1
1
1
1
LSTYCPR + Carbamidomethyl (C)
TPEVDDEALEKFDK
VAALGSDPHISMK
ALMLGGRPIVK + Oxidation (M)
YVVVAR
DLVAPYAVAAR
LTNSGTKTAQLISR
VATAISFR
GLPAGLHGFHVHQNADCGATEK + Acrylamide (C)
GSYLSVITSNVNGLNAPTK
135
224
155
344
404
134
55
337
338
125
123
306
223
58
564.2641
692.3082
583.8251
931.9608
777.3744
563.7855
465.7663
900.4903
600.6627
552.2544
547.7538
842.9005
691.8168
467.7302
1126.5136
1382.6018
1165.6356
1861.9070
2329.1014
1125.5564
929.5180
1798.9660
1798.9663
1102.4942
1093.4930
1683.7864
1381.6190
933.4458
1126.5151
1382.6039
1165.6356
1861.9100
2329.1022
1125.5567
929.5182
1798.9651
1798.9611
1102.4964
1093.4896
1683.7886
1381.6233
933.4491
-1.29
-1.50
0.01
-1.57
-0.36
-0.23
-0.17
0.51
2.87
-1.99
3.14
-1.29
-3.05
-3.45
0
1
0
1
0
1
0
0
0
0
0
1
1
1
15
14
14
14
14
14
14
14
14
14
14
14
14
14
16
28
25
42
43
47
72
33
33
18
32
49
35
46
1
1
1
1
1
1
1
1
1
1
1
1
1
1
DYCVVMVNGK
MMMMRPDAGRR + 2 Oxidation (M)
VGPNLHGIFGR
IKCDETLPACLNCLK + 2 Acrylamide (C); Carbamidomethyl (C)
RFSGEVFER
VDSNGVLVK
DVAMKPPSVYLLPPTR + Oxidation (M)
LAVEGIGAVMLTGDNALR
ELMANGEDPK
MDDLQAVMR + Oxidation (M)
MFICYKFMDK + Carbamidomethyl (C)
MDFTHRK
250
203
382
259
78
228
63
179
80
174
144
403
372
38
211
204
147
279
285
119
90
195
365
336
377
345
227
91
748.3270
640.8817
1042.0090
752.8675
494.7202
696.8501
475.7286
615.2520
496.7979
610.7991
382.5555
777.3744
970.9705
430.2658
655.3226
427.5905
574.7760
788.3854
802.4017
540.7463
505.2690
630.2972
484.5099
897.9111
1006.4900
621.6436
695.8461
505.8158
1494.6394
1279.7488
2082.0034
1503.7204
987.4258
1391.6856
949.4426
1228.4894
991.5812
1219.5836
1144.6447
2329.1014
1939.9264
858.5170
1308.6306
1279.7497
1147.5374
1574.7562
1602.7888
1079.4780
1008.5234
1258.5798
1934.0105
1793.8076
2010.9654
1861.9090
1389.6776
1009.6170
1494.6362
1279.7500
2081.9953
1503.7173
987.4266
1391.6827
949.4440
1228.4886
991.5814
1219.5808
1144.6452
2329.1022
1939.9284
858.5174
1308.6310
1279.7500
1147.5397
1574.7610
1602.7890
1079.4740
1008.5240
1258.5798
1934.0118
1793.8077
2010.9608
1861.9105
1389.6736
1009.6172
2.19
-0.89
3.93
2.06
-0.77
2.11
-1.39
0.69
-0.19
2.33
-0.45
-0.36
-1.02
-0.46
-0.23
-0.24
-1.95
-3.01
-0.07
3.79
-0.53
0.02
-0.66
-0.01
2.30
-0.82
2.94
-0.11
0
1
1
1
1
1
0
0
1
0
0
0
0
1
1
1
0
0
0
0
1
0
1
0
1
1
1
0
14
13
13
13
13
13
13
13
13
13
13
13
13
13
13
12
12
12
12
12
12
12
11
11
11
11
11
11
20
7.9
49
51
13
65
34
7.6
20
71
48
63
63
54
57
13
54
74
75
53
63
48
65
81
89
87
93
14
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
IEEELDDFEDNK
VVQGEPSLKVPK
ADPPDPVSVLEASGWRCAR + Carbamidomethyl (C)
RALTENCECPVK + 2 Acrylamide (C)
CPMDKHNK + Oxidation (M)
QSVRDNGTMVAAK + Oxidation (M)
MFAPGNSAR
CCTEMSELR + 2 Acrylamide (C); Oxidation (M)
ARALVAYTK
WQDAGFLPMR
VIDSAATTLVR
MQYDAAVVGAGPAGLMFAR + Oxidation (M)
VATAIEKK
ARNDEYEIATK
VVQGEPSLKVPK
EPEPEPEPPK
EQPSLESMLELQR + Oxidation (M)
SPIFGPEEVTSVEGR
MDLGEQMTR
ETLASYGRL
CAQMLADHGVK + Acrylamide (C); Oxidation (M)
HMYIRDVSVLITNMVK + Oxidation (M)
CITEIGCPGIGFDADAR + Carbamidomethyl (C)
WIIFDSDNELDLMEKK + Oxidation (M)
NEEGEVGFRVLVGGGMGR
TIIIEDNRSTDN
EAGIPVIALK
347
189
362
37
308
70
209
283
371
149
51
278
128
369
626.9752
627.2769
484.5097
428.7784
849.4440
486.7224
430.2427
532.2820
970.9688
575.7675
452.2867
785.8735
555.7515
968.0128
1877.9038
1252.5392
1934.0097
855.5422
1696.8734
971.4302
1287.7063
1593.8242
1939.9230
1149.5204
902.5588
1569.7324
1109.4884
1934.0110
1877.9056
1252.5434
1934.0084
855.5430
1696.8784
971.4270
1287.7043
1593.8259
1939.9166
1149.5244
902.5589
1569.7279
1109.4845
1934.0109
-0.96
-3.32
0.67
-0.82
-2.94
3.37
1.57
-1.07
3.33
-3.40
-0.09
2.87
3.53
0.08
0
0
0
0
0
0
1
0
1
0
1
0
0
0
11
11
11
11
11
11
11
11
10
10
10
10
10
10
83
46
73
24
87
31
75
1.1e+002
1e+002
54
28
90
72
97
1
1
1
1
1
1
1
1
1
1
1
1
1
1
LMQFTYMIFSQLQNV + Oxidation (M)
EPDPNAFYGLM
SERPVWGTCAGLIFLSK + Acrylamide (C)
IIGVVVEK
VPLDASLPLWNDTTR
EDYSMALK + Oxidation (M)
NIPKSLCMLAK + Acrylamide (C)
TALLGCTQMPFVVK + Acrylamide (C); Oxidation (M)
CSVTLCDGVAGCGKTTAIK + 2 Carbamidomethyl (C)
APWDGGFSWK
TKIPAVFK
AGHMVPSDQGEMALK
DASMVLGMDR + Oxidation (M)
GSYLSVITSNVNGLNAPTK
368
300
360
136
296
359
222
325
289
645.6776
834.9272
965.4530
564.2776
823.9044
964.9622
691.3295
883.8953
545.6295
1934.0110
1667.8398
1928.8914
1126.5406
1645.7942
1927.9098
1380.6444
1765.7760
1633.8667
1934.0118
1667.8366
1928.8938
1126.5441
1645.7981
1927.9051
1380.6442
1765.7802
1633.8610
-0.41
1.94
-1.23
-3.05
-2.33
2.47
0.15
-2.34
3.48
1
0
1
0
1
0
0
1
1
10
10
10
10
10
10
10
10
10
99
1.3e+002
1.1e+002
96
1.3e+002
1e+002
1.4e+002
60
1.1e+002
1
1
1
1
1
1
1
1
1
HMYIRDVSVLITNMVK + Oxidation (M)
IIPSAEDPSQDIVER
MDALEAKFAGGFGQQTDK + Oxidation (M)
NPTELESMEEALRK
YVLDIDYDVNLDETNK
DLETSIATMQEK + Oxidation (M)
WECVSGSGTKNSNQNR
SLEFSRVPLSCVAR + Acrylamide (C)
12/9/2009
Page 14 of 15
140
411
11
408
206
35
318
351
364
148
207
568.2492
583.2830
399.1929
777.3746
641.8489
428.2612
578.9454
481.0310
645.6774
575.7674
642.3408
1134.4838
2329.1029
796.3712
2329.1020
1281.6832
854.5078
1733.8144
1920.0949
1934.0104
1149.5202
1282.6670
1134.4872
2329.1116
796.3715
2329.1022
1281.6829
854.5086
1733.8141
1920.0931
1934.0143
1149.5165
1282.6670
-2.91
-3.74
-0.36
-0.10
0.24
-0.90
0.15
0.91
-2.01
3.29
0.07
0
1
0
0
1
1
1
0
1
1
1
10
10
9
9
9
9
9
9
9
9
9
41
1.5e+002
60
1.4e+002
1e+002
54
1.2e+002
18
1.1e+002
90
1.2e+002
1
1
1
1
1
1
1
1
1
1
1
CSMCDIPPK + 2 Acrylamide (C)
STDKPTMICCKTIIGYGSPNK + Carbamidomethyl (C); Oxidation (M)
SGTDYVR
NFATIREYLR
IRGAVPSR
IMENQSEELEEKQK
EQVLDAIVALIAAAVPEAK
DQMLTDLTSLLKINSAR + Oxidation (M)
CPPVEEYKW
VFLKYGASNER
156
286
316
261
319
233
363
307
282
132
7
52
262
199
584.2982
810.8329
867.9091
503.9201
869.4684
709.8160
645.6774
843.3921
797.9095
561.2606
392.7485
453.7556
755.3774
635.2742
1166.5818
1619.6512
1733.8036
1508.7385
1736.9222
1417.6174
1934.0104
1684.7696
1593.8044
1120.5066
783.4824
905.4966
1508.7402
1268.5338
1166.5787
1619.6452
1733.8083
1508.7359
1736.9243
1417.6177
1934.0109
1684.7661
1593.8007
1120.5079
783.4854
905.4970
1508.7446
1268.5343
2.67
3.73
-2.68
1.73
-1.20
-0.19
-0.26
2.09
2.35
-1.12
-3.80
-0.43
-2.88
-0.36
1
0
0
0
1
0
1
1
0
0
1
1
0
1
9
9
9
9
9
9
8
8
8
8
8
8
8
8
1e+002
19
1.3e+002
1.7e+002
97
82
1.3e+002
99
1.8e+002
96
1.1e+002
2.6e+002
2e+002
65
1
1
1
1
1
1
1
1
1
1
1
1
1
1
SLMKNIASCK + Carbamidomethyl (C); Oxidation (M)
MQDGQFMNDLMMK + 2 Oxidation (M)
YLETFANFSGADMLR
QIGDTISDIDASFK
GPMSLRSPTPKPGTPAK + Oxidation (M)
MTASTSSMAASSVR + 2 Oxidation (M)
QQYGEGIATAVKQTLEAK
VQNLAHDCGDKCPTK + Carbamidomethyl (C)
HCVLPEPAATAGMVK + Acrylamide (C)
MLMVHQSML + 2 Oxidation (M)
VLLPTKN
IEKAFGNK
AHMNLGFVFTTQK + Oxidation (M)
FIDDRDEECK
142
373
397
170
129
110
334
374
124
384
381
383
54
370
413
400
190
272
402
191
281
287
346
335
410
217
396
242
571.8611
647.6496
1144.0840
600.2911
556.3163
523.7767
894.4148
972.9598
550.2448
1044.4690
691.3157
696.6472
458.7880
645.6780
782.7076
772.7025
627.3529
769.3237
772.7036
627.7686
794.8646
810.8340
621.9719
897.9102
777.3749
679.3635
761.7321
728.3643
1141.7076
1939.9270
2286.1534
1198.5676
1110.6180
1045.5388
1786.8150
1943.9050
1098.4750
2086.9234
2070.9253
2086.9198
915.5614
1934.0122
2345.1010
2315.0857
1252.6912
1536.6328
2315.0890
1253.5226
1587.7146
1619.6534
1862.8939
1793.8058
2329.1029
1356.7124
2282.1745
1454.7140
1141.7070
1939.9212
2286.1505
1198.5652
1110.6145
1045.5404
1786.8196
1943.9089
1098.4764
2086.9261
2070.9330
2086.9160
915.5641
1934.0124
2345.0957
2315.0813
1252.6961
1536.6288
2315.0807
1253.5256
1587.7095
1619.6596
1862.8920
1793.8005
2329.0961
1356.7071
2282.1762
1454.7149
0.54
2.97
1.29
2.03
3.18
-1.45
-2.53
-2.00
-1.21
-1.29
-3.75
1.80
-2.87
-0.14
2.23
1.87
-3.90
2.63
3.57
-2.35
3.25
-3.78
1.02
3.00
2.90
3.94
-0.77
-0.58
1
1
1
1
1
0
0
0
1
0
0
0
0
1
1
1
1
0
1
1
1
0
1
0
1
1
1
1
7
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
5
5
5
35
2.1e+002
2e+002
1.2e+002
1.4e+002
2.7e+002
1.6e+002
2e+002
61
1.3e+002
1.6e+002
1.3e+002
16
2.2e+002
2.4e+002
2.4e+002
1.7e+002
53
2.7e+002
82
1.8e+002
40
3.8e+002
3.4e+002
4e+002
4.4e+002
3e+002
3.3e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
QKEAVLTILK
FYFYKELEQICCQVK
EKNDHYNNAIAGAFGGAVLGLR
CTGTSKEFAAK + Carbamidomethyl (C)
LRGAEPAELR
EAAAVTSEIR
DTCENQNYLNYLLK + Carbamidomethyl (C)
CAMIMITCNGIAYAIAR + 2 Carbamidomethyl (C); Oxidation (M)
KCYDAEQSR
LVVCDCSEMDVDEFVALGK + Oxidation (M)
MDQPIGGFGGNALHDSFHR + Oxidation (M)
TQSGISHDAMMAAIHENSR + 2 Oxidation (M)
LLLTTDLK
AFIVGCKFADPQAVFPPK
YDTKMSTEHNATQPLPDELR
DGEEAECVLFFSKQDIMINK
ALEKLHAMVNK
NSDVSSANENGSDNK
MTLLVAMITGCSSNSASDKGEK + Carbamidomethyl (C); Oxidation (M)
MKXDCLAXPVR
YCDCLKAMQELQK + Oxidation (M)
NGECVYGCDYAGLEK
IMGAAWSAPTWMKSNGR
VISMGSDFDYVWTMK + Oxidation (M)
TIDEFENKIEDQETYLQSK
LPLMSEPRTGEK
MHQAIPCGLLENATVTAEIKK + Oxidation (M)
LMEKMGLDDYLK
68
385
380
265
176
349
412
200
348
320
33
270
333
392
480.8015
723.0741
1029.5040
758.3265
611.7319
941.4268
782.7053
637.3257
939.9598
873.4121
425.7608
510.5974
596.6121
738.0331
959.5884
2166.2005
2056.9934
1514.6384
1221.4492
1880.8390
2345.0941
1272.6368
1877.9050
1744.8096
849.5070
1528.7704
1786.8145
2211.0775
959.5916
2166.2008
2056.9863
1514.6350
1221.4464
1880.8332
2345.0998
1272.6350
1877.9115
1744.8130
849.5072
1528.7674
1786.8156
2211.0744
-3.30
-0.15
3.47
2.30
2.31
3.13
-2.44
1.43
-3.41
-1.93
-0.22
1.94
-0.61
1.38
1
1
0
1
0
0
0
0
0
0
1
1
0
1
5
4
4
4
4
4
4
4
4
4
4
4
4
3
52
1e+002
4.1e+002
1e+002
7.2
2.6e+002
4.4e+002
3.5e+002
4.7e+002
3.5e+002
2.5e+002
5.2e+002
3.2e+002
6e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
FNRAVLLK
LLPTNAVEILQTSGDRAQIK
MFDMHSVFIVDDHPVIR
GKLMMMTACDDGR + Acrylamide (C); Oxidation (M)
CSGESVCSYCK + Carbamidomethyl (C)
GAMPLIHGSQGCSTYMR + Carbamidomethyl (C); Oxidation (M)
SHVSAYPPGQLLFTPDECQEK
YYTTVGNGTAVK
IMGIDCTPTSDGIIIEGK + Oxidation (M)
YNVDIWAACAYELSK
RIPTPIPG
VPWYSGSYLKSSR
HVDLSSPEMAQAETTR + Oxidation (M)
KLSVMFPMVSEPWEFDAAK
77
251
257
398
328
194
98
236
162
494.3004
748.3857
751.3196
763.0585
591.2808
629.3287
510.3085
712.8190
590.7462
986.5862
1494.7568
1500.6246
2286.1537
1770.8206
1256.6428
1018.6024
1423.6234
1179.4778
986.5873
1494.7541
1500.6225
2286.1525
1770.8182
1256.6401
1018.6036
1423.6249
1179.4761
-1.02
1.86
1.45
0.50
1.36
2.18
-1.14
-1.02
1.52
1
0
0
1
0
1
0
1
1
3
3
2
2
2
2
2
2
2
2.4e+002
6.2e+002
1.5e+002
6.8e+002
5.1e+002
6.9e+002
1.7e+002
4e+002
84
1
1
1
1
1
1
1
1
1
VLIANRSSK
AGAAPSFYPLTCGLK
EDADTAEFMGMLR + Oxidation (M)
LLGGPGEGMNKIENLTEIETR + Oxidation (M)
HVCSPNVEHLTGYMK + Carbamidomethyl (C)
KSVDGYSGAVFK
HVGAGKPVVR
TDESNVCKSENK + Acrylamide (C)
CCDKNDANR + 2 Acrylamide (C)
12/9/2009
79
44
105
60
3
4
5
6
13
20
39
496.7211
436.7748
516.3145
472.2877
357.6367
357.6368
362.2222
384.2354
406.2484
414.7617
432.1425
991.4276
871.5350
1030.6144
942.5608
713.2588
713.2590
722.4298
766.4562
810.4822
827.5088
862.2704
50
130
146
271
274
326
416
450.2745
559.8731
574.2831
766.3235
777.8378
591.2408
981.3895
898.5344
1117.7316
1146.5516
1530.6324
1553.6610
1770.7006
2941.1467
991.4281
871.5378
1030.6175
942.5610
-0.41
-3.21
-2.95
-0.20
0
0
1
1
1
1
1
0
2.5e+002
4.4e+002
2.9e+002
9.1e+002
Page 15 of 15
1
1
1
1
MDDTPLER + Oxidation (M)
EISVIALK
ANTPILKFK
SRSKPIQK
Search Parameters
Type of search
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
MS/MS Ion Search
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
416
12/9/2009
Page 1 of 10
User
Email
Search title
MS data file
Database
Timestamp
Protein hits
:
:
:
:
:
:
:
LTQFT Daemon
D:\Data\Mark\18_7-09\N_18_7_09_6.RAW
9 Dec 2009 at 04:03:32 GMT
gi|157165691 C4-dicarboxylate-binding periplasmic protein [Campylobacter concisus 13826]
gi|1351907
gi|494583
gi|1942778
gi|122411
gi|6320157
gi|136429
gi|386854
type II keratin subunit protein
gi|157165454 amino acid carrier protein AlsT [Campylobacter concisus 13826]
gi|538275
gi|165945
immunoglobulin mu chain
gi|157165413 superoxide dismutase (Fe) [Campylobacter concisus 13826]
gi|291470
lambda-immunoglobulin
gi|1212992
titin [Homo sapiens]
gi|3914346
gi|157164710 molybdenum cofactor synthesis domain-containing protein [Campylobacter concisus 13826]
gi|19168448 autoinducer-2 synthase; LuxS [Campylobacter coli]
gi|256811403 DNA-directed DNA polymerase [Methanocaldococcus fervens AG86]
gi|123506
gi|157165403 translation initiation factor IF-3 [Campylobacter concisus 13826]
gi|157164523 putative outer membrane protein [Campylobacter concisus 13826]
16
0
0.00 %
29
0
0.00 %
Format As
Standard scoring
Show sub-sets 0
Require bold red
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1.
Help
Peptide Summary
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MudPIT scoring
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Error tolerant
Archive Report
gi|157165691
Mass: 18808
Score: 265
Queries matched: 12
emPAI: 1.28
C4-dicarboxylate-binding periplasmic protein [Campylobacter concisus 13826]
Query
7
80
93
94
Observed
390.2184
500.2481
510.2687
510.2688
Mr(expt)
778.4222
998.4816
1018.5228
1018.5230
Mr(calc)
778.4225
998.4821
1018.5236
1018.5236
ppm Miss Score
-0.29
0
26
-0.48
0
28
-0.77
0
(27)
-0.58
0
40
147
158
613.7896
621.7872
1225.5646
1241.5598
1225.5649
1241.5598
-0.19
0.04
0
0
Expect Rank
4.4
1
1.5
1
3.7
1
0.21
1
62 0.00047
(62) 0.00044
1
1
Peptide
K.INYELK.N
K.FHIDNPEK.Q
K.FQWTGGPVK.K.FQWTGGPVK.K.MDTGVGNYELK.F
K.MDTGVGNYELK.F + Oxidation (M)
12/9/2009
188
189
237
238
239
245
2.
474.2523
710.8751
665.6414
670.9730
1005.9570
713.6716
1419.7351
1419.7356
1993.9024
2009.8972
2009.8994
2137.9930
1419.7357
1419.7357
1993.9026
2009.8976
2009.8976
2137.9925
-0.48
-0.07
-0.13
-0.19
0.94
0.22
1
1
0
0
0
1
(27)
51
(20)
31
(20)
26
2.6
0.0083
7
0.47
6.1
2.4
1
1
1
1
1
1
Page 2 of 10
K.INYELKNLDNGK.T
K.INYELKNLDNGK.T
K.GTFMPMVASDGPHYGANVK.M + Oxidation (M)
K.GTFMPMVASDGPHYGANVK.M + 2 Oxidation (M)
K.GTFMPMVASDGPHYGANVK.M + 2 Oxidation (M)
K.KGTFMPMVASDGPHYGANVK.M + 2 Oxidation (M)
gi|1351907
Mass: 69248
Score: 197
Queries matched: 6
emPAI: 0.05
Query
81
90
133
177
192
217
Observed
501.7950
507.8131
582.3190
653.3618
722.3242
820.4729
Mr(expt)
1001.5754
1013.6116
1162.6234
1304.7090
1442.6338
1638.9312
Mr(calc)
1001.5757
1013.6121
1162.6234
1304.7088
1442.6347
1638.9305
-0.27
0
47
0.019
1
-0.42
0
39
0.072
1
0.07
0
32
0.84
1
0.17
0
31
0.87
1
-0.63
0
29
1
1
0.47
1
20
2.5
1
Peptide
K.LVNELTEFAK.T
K.HLVDEPQNLIK.Q
K.YICDNQDTISSK.L + Carbamidomethyl (C)
R.KVPQVSTPTLVEVSR.S
gi|30794280
Mass: 69278
Score: 197
Queries matched: 6
gi|74267962
Mass: 69190
Score: 197
Queries matched: 6
gi|154425704
Mass: 69248
Score: 197
Queries matched: 6
3.
gi|494583
Mass: 15714
Score: 167
Queries matched: 7
emPAI: 0.22
Query
16
74
75
76
77
78
114
Observed
414.2708
497.7818
497.7818
497.7819
499.8031
499.8032
541.7648
Mr(expt)
826.5270
993.5490
993.5490
993.5492
997.5916
997.5918
1081.5150
Mr(calc)
826.5276
993.5495
993.5495
993.5495
997.5920
997.5920
1081.5152
-0.71
0
33
0.29
1
-0.44
1
38
0.14
1
-0.44
1
(30)
0.89
1
-0.24
1
(36)
0.22
1
-0.39
0
(48)
0.01
1
-0.19
0
60 0.00061
1
-0.18
0
35
0.35
1
Peptide
-.VQAVAVLK.G
K.GSFKDSLIK.L
K.GSFKDSLIK.L
K.GSFKDSLIK.L
K.LIGPTSVVGR.S
K.LIGPTSVVGR.S
K.THGAPTDEVR.H
gi|6322564
Mass: 15845
Score: 167
Queries matched: 7
gi|6730103
Mass: 15680
Score: 167
Queries matched: 7
gi|15826571
Mass: 15724
Score: 167
Queries matched: 7
Mass: 15944
Score: 167
Queries matched: 7
gi|27573538
gi|27573539
Mass: 15836
Score: 167
Queries matched: 7
Mass: 15811
Score: 167
Queries matched: 7
gi|27573540
4.
gi|1942778
Mass: 15089
Score: 159
Queries matched: 4
emPAI: 0.23
Query
14
107
127
198
Observed
409.7238
529.2728
573.3298
750.3661
Mr(expt)
817.4330
1056.5310
1144.6450
1498.7176
Mass:
gi|146149083
gi|146149272
Mass:
5.
Mr(calc)
817.4334
1056.5314
1144.6452
1498.7164
-0.44
0
27
1.3
1
-0.35
0
39
0.11
1
-0.11
1
48
0.013
1
0.80
0
45
0.032
1
15220
Score: 159
caballus]
15220
Score: 159
caballus]
Peptide
R.VDPVNFK.L
-.VLSAADKTNVK.A
K.VGGHAGEFGAEALER.M
Queries matched: 4
Queries matched: 4
gi|122411
Mass: 15236
Score: 149
Queries matched: 5
Query
14
107
Observed
409.7238
529.2728
Mr(expt)
817.4330
1056.5310
Mr(calc)
817.4334
1056.5314
-0.44
0
27
1.3
1
-0.35
0
39
0.11
1
Peptide
R.VDPVNFK.L
12/9/2009
127
204
205
573.3298
505.9110
758.3636
1144.6450
1514.7112
1514.7126
1144.6452
1514.7113
1514.7113
-0.11
-0.12
0.86
1
0
0
48
(28)
35
0.013
1.2
0.28
1
1
1
Page 3 of 10
M.VLSAADKTNVK.A
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
gi|230479
Mass: 15105
Score: 149
Queries matched: 5
Mass: 15105
Score: 149
Queries matched: 5
gi|230632
gi|313500
Mass: 15236
Score: 149
Queries matched: 5
gi|24158717
Mass: 15105
Score: 149
Queries matched: 5
6.
gi|157165213
Mass: 19144
Score: 142
Queries matched: 5
emPAI: 0.18
Query
57
110
111
194
202
7.
Observed
475.7506
360.2010
539.7980
726.3651
755.8911
Mr(expt)
949.4866
1077.5812
1077.5814
1450.7156
1509.7676
Mr(calc)
949.4869
1077.5818
1077.5818
1450.7151
1509.7675
-0.23
0
33
1.2
2
-0.60
1
33
0.64
1
-0.34
1
(23)
7
1
0.38
1
25
5.1
1
0.11
1
51 0.0075
1
gi|115495209
Mass: 59194
Score: 137
Queries matched: 5
Peptide
K.ADAIIEYR.K
K.ADAIIEYRK.A
K.ADAIIEYRK.A
K.GEISTTGKSEVSEK.A
K.TFDNLKGEISTTGK.S
emPAI: 0.06
Query
12
45
131
135
164
8.
Observed
403.7113
443.7279
580.7899
587.7980
626.2783
Mr(expt)
805.4080
885.4412
1159.5652
1173.5814
1250.5420
Mr(calc)
805.4083
885.4419
1159.5656
1173.5812
1250.5424
-0.29
0
22
5.6
2
-0.70
0
40
0.072
1
-0.26
0
27
2.3
1
0.22
0
(27)
2.7
1
-0.27
0
48 0.0088
1
Peptide
R.FVGDGGVR.M
R.FSVQMFK.F
R.LEGTPQCSLR.Y + Carbamidomethyl (C)
R.MPEDCVPTFR.C + Carbamidomethyl (C)
gi|6320157
Mass: 19051
Score: 121
Queries matched: 7
emPAI: 0.38
Query
1
152
153
154
155
156
182
Observed
353.7156
617.8555
617.8557
617.8558
617.8561
617.8562
681.9032
Mr(expt)
705.4166
1233.6964
1233.6968
1233.6970
1233.6976
1233.6978
1361.7918
Mr(calc)
705.4174
1233.6969
1233.6969
1233.6969
1233.6969
1233.6969
1361.7918
-1.01
0
26
2.7
4
-0.35
0
59 0.00066
1
-0.03
0
(39)
0.079
1
0.13
0
(42)
0.035
1
0.62
0
(50) 0.0055
1
0.78
0
(33)
0.28
1
0.01
1
36
0.058
1
Peptide
K.YVVVAR.A
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.KIVEIPGEVPPGK.Y
gi|151941952
Mass: 19021
Score: 121
Queries matched: 7
gi|190405057
Mass: 19007
Score: 121
Queries matched: 7
9.
gi|136429
Mass: 24394
Score: 110
Queries matched: 5
emPAI: 0.14
Query
21
22
23
104
250
Observed
421.7581
421.7582
421.7582
523.2853
737.7062
Mr(expt)
841.5016
841.5018
841.5018
1044.5560
2210.0968
Mr(calc)
841.5022
841.5022
841.5022
1044.5564
2210.0967
-0.60
0
56 0.0042
1
-0.36
0
(45) 0.048
1
-0.36
0
(27)
3.1
1
-0.29
0
34
0.52
1
0.02
0
21
10
1
Peptide
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
K.LSSPATLNSR.V
gi|494360
Mass: 23458
Score: 110
Queries matched: 5
gi|999627
Mass: 8814
Score: 110
Queries matched: 5
gi|1942351
Mass: 13284
Score: 110
Queries matched: 5
Mass: 23460
Score: 110
Queries matched: 5
gi|2914482
gi|3318722
Mass: 23457
Score: 110
Queries matched: 5
12/9/2009
Page 4 of 10
gi|110590762
Mass: 23458
Score: 110
Queries matched: 5
gi|157878102
Mass: 24157
Score: 110
Queries matched: 5
gi|242253868
Mass: 25865
Score: 110
Queries matched: 5
10.
gi|157165326
Mass: 18857
Score: 96
Queries matched: 4
emPAI: 0.18
Query
15
29
64
183
11.
Observed
410.7244
425.7299
486.7899
685.8585
Mr(expt)
819.4342
849.4452
971.5652
1369.7024
Mr(calc)
819.4347
849.4452
971.5651
1369.7030
gi|386854
Mass: 52757
Score: 85
type II keratin subunit protein
-0.51
0
22
7.6
4
0.03
0
(21)
9.2
2
0.14
1
41
0.095
1
-0.43
0
33
0.6
1
Peptide
R.MLCGVIK.- + Carbamidomethyl (C)
R.MLCGVIK.- + Acrylamide (C); Oxidation (M)
K.IKNLDELK.G
K.TNYGVAFFPNLK.G
Queries matched: 3
Query
65
103
187
Observed
487.2692
517.2616
697.3694
Mr(expt)
972.5238
1032.5086
1392.7242
Mr(calc)
972.5240
1032.5087
1392.7249
-0.13
0
34
0.77
1
-0.08
0
20
9.5
1
-0.45
1
31
0.87
1
Peptide
K.IEISELNR.M
R.TLLEGEESR.M
R.TNAENEFVTIKK.D
gi|7331218
Mass: 65978
Score: 85
Queries matched: 3
gi|11935049
Mass: 66027
Score: 85
Queries matched: 3
gi|109096823
Mass: 65170
Score: 85
Queries matched: 3
Mass: 64332
Score: 85
Queries matched: 3
gi|109096825
Mass: 62980
Score: 85
Queries matched: 3
gi|109096827
gi|109096829
Mass: 61838
Score: 85
Queries matched: 3
gi|109096833
Mass: 61566
Score: 85
Queries matched: 3
gi|109096835
Mass: 58846
Score: 85
Queries matched: 3
gi|119395750
Mass: 65999
Score: 85
Queries matched: 3
gi|160961491
Mass: 65450
Score: 85
Queries matched: 3
12.
gi|157165454
Mass: 19609
Score: 77
Queries matched: 2
amino acid carrier protein AlsT [Campylobacter concisus 13826]
Query
63
162
13.
Observed
485.8056
625.8221
Mr(expt)
969.5966
1249.6296
Mr(calc)
969.5971
1249.6302
gi|538275
Mass: 22697
Score: 77
-0.49
0
47
0.017
1
-0.46
0
30
1.4
1
Queries matched: 2
Peptide
K.VVLVGNLTR.D
K.SAEIANQYLNK.G
emPAI: 0.15
Query
51
122
Observed
465.7663
561.3136
Mr(expt)
929.5180
1120.6126
Mr(calc)
929.5182
1120.6128
-0.15
0
21
16
3
-0.16
0
57
0.002
1
Peptide
K.LTPETLTR.W
K.DTQSIIFLGK.V
gi|1245695
Mass: 28553
Score: 77
Queries matched: 2
gi|31340900
Mass: 46175
Score: 77
Queries matched: 2
gi|126165236
Mass: 46368
Score: 77
Queries matched: 2
gi|160332365
Mass: 46208
Score: 77
Queries matched: 2
gi|226373737
Mass: 46208
Score: 77
Queries matched: 2
14.
gi|165945
Mass: 53512
Score: 69
Queries matched: 3
12/9/2009
Page 5 of 10
Query
116
119
169
Observed
547.2820
554.2892
633.8093
Mr(expt)
1092.5494
1106.5638
1265.6040
Mr(calc)
1092.5485
1106.5641
1265.6040
0.86
0
28
2.1
1
-0.27
0
(26)
3.8
1
0.01
0
41
0.1
1
Peptide
K.ALETHTYFER.H
Mass: 64480
Score: 69
Queries matched: 3
gi|478694
Ig mu chain - sheep
gi|561876
Mass: 52124
Score: 69
Queries matched: 3
This CDS feature is included to show the translation of the corresponding C_region. Presently translation qualifiers on C_region features are illegal
gi|2232299
Mass: 47885
Score: 69
Queries matched: 3
gi|3834667
Mass: 48183
Score: 69
Queries matched: 3
gi|24496448
Mass: 65621
Score: 69
Queries matched: 3
gi|33413902
Mass: 49106
Score: 69
Queries matched: 3
gi|34538498
Mass: 49909
Score: 69
Queries matched: 3
gi|162424563
Mass: 52197
Score: 69
Queries matched: 3
immunoglobulin mu heavy chain constant region [Capra hircus]
15.
gi|157165180
Mass: 20941
Score: 65
Queries matched: 2
Query
28
59
16.
Observed
424.7657
478.7435
Mr(expt)
847.5168
955.4724
Mr(calc)
847.5167
955.4723
0.15
0
36
0.19
1
0.15
0
29
0.59
1
gi|157165413
Mass: 23563
Score: 64
Queries matched: 2
superoxide dismutase (Fe) [Campylobacter concisus 13826]
Peptide
R.IISVAFAK.T
K.HSSDVNLGK.-
emPAI: 0.14
Query
150
151
17.
Observed
615.3060
615.8566
Mr(expt)
1228.5974
1229.6986
gi|291470
Mass: 19537
Mr(calc)
1228.5976
1229.6979
-0.10
0
35
0.27
1
0.59
1
29
0.96
1
Score: 63
Queries matched: 1
Peptide
K.EGLGSVEFYTK.E
K.KLEIVQTSNAK.T
emPAI: 0.17
Query
196
Observed
738.8684
Mr(expt)
1475.7222
Mr(calc)
1475.7216
0.42
0
63 0.00051
1
Peptide
K.VNDAVTTDGVQTTR.S
Mass: 17423
Score: 63
Queries matched: 1
gi|291474
gi|291478
Mass: 22654
Score: 63
Queries matched: 1
gi|9858133
Mass: 24186
Score: 63
Queries matched: 1
immunoglobulin G light chain [Equus caballus]
gi|194214037
Mass: 17257
Score: 63
Queries matched: 1
PREDICTED: similar to lambda-immunoglobulin [Equus caballus]
gi|194214039
Mass: 15452
Score: 63
Queries matched: 1
18.
gi|62126072
Mass: 46687
Score: 61
Queries matched: 2
emPAI: 0.07
Query
51
113
Observed
465.7663
540.2957
Mr(expt)
929.5180
1078.5768
Mr(calc)
929.5182
1078.5771
-0.15
0
21
16
3
-0.22
0
40
0.11
1
Peptide
K.LTPETLTR.W
R.HIDELSLPR.F
gi|194676990
Mass: 45494
Score: 61
Queries matched: 2
PREDICTED: similar to endopin 2B [Bos taurus]
19.
gi|1212992
Mass: 2991589
Score: 59
Queries matched: 2
12/9/2009
Query
18
24
Observed
417.7288
421.7711
Mr(expt)
833.4430
841.5276
Mr(calc)
833.4429
841.5273
0.16
0
35
0.62
1
0.42
0
25
1.9
2
Page 6 of 10
Peptide
R.LQSMTVR.Q
K.LTIPAVTK.A
gi|3928489
Mass: 222566
Score: 59
Queries matched: 2
titin [Oryctolagus cuniculus]
Mass: 2991182 Score: 59
Queries matched: 2
gi|17066104
N2B-Titin Isoform [Homo sapiens]
gi|17066105
Mass: 3813840 Score: 59
Queries matched: 2
Titin [Homo sapiens]
gi|17066106
Mass: 631230
Score: 59
Queries matched: 2
Novex-3 Titin Isoform [Homo sapiens]
Mass: 32739
Score: 59
Queries matched: 2
gi|33871368
TTN protein [Homo sapiens]
gi|37573987
Mass: 60186
Score: 59
Queries matched: 2
gi|47124504
Mass: 69051
Score: 59
Queries matched: 2
gi|85543964
Mass: 21554
Score: 59
Queries matched: 2
Chain A, Crystal Structure Of The Titin Domains Z1z2 In Complex With Telethonin
gi|108861911
Mass: 3813810 Score: 59
Queries matched: 2
RecName: Full=Titin; AltName: Full=Connectin; AltName: Full=Rhabdomyosarcoma antigen MU-RMS-40.14
gi|110349713
Mass: 3004958 Score: 59
Queries matched: 2
titin isoform novex-1 [Homo sapiens]
gi|110349715
Mass: 2991151 Score: 59
Queries matched: 2
titin isoform N2-B [Homo sapiens]
Mass: 3012156 Score: 59
Queries matched: 2
gi|110349717
gi|110349719
Mass: 3711359 Score: 59
Queries matched: 2
titin isoform N2-A [Homo sapiens]
gi|110349721
Mass: 631174
Score: 59
Queries matched: 2
gi|110590764
Mass: 20706
Score: 59
Queries matched: 2
Chain A, Crystal Structure Of The N-Terminus Of Titin
gi|119631418
Mass: 3878844 Score: 59
Queries matched: 2
titin, isoform CRA_a [Homo sapiens]
Mass: 2991188 Score: 59
Queries matched: 2
gi|119631420
titin, isoform CRA_b [Homo sapiens]
gi|119631421
Mass: 631293
Score: 59
Queries matched: 2
titin, isoform CRA_c [Homo sapiens]
gi|119631423
Mass: 3004995 Score: 59
Queries matched: 2
titin, isoform CRA_d [Homo sapiens]
Mass: 3012223 Score: 59
Queries matched: 2
gi|119631424
titin, isoform CRA_e [Homo sapiens]
gi|194043971
Mass: 147632
Score: 59
Queries matched: 2
PREDICTED: similar to Titin (Connectin) (Rhabdomyosarcoma antigen MU-RMS-40.14) [Sus scrofa]
gi|225131084
Mass: 3711397 Score: 59
Queries matched: 2
Mass: 3708275 Score: 58
Queries matched: 2
gi|194222358
PREDICTED: similar to titin [Equus caballus]
20.
gi|3914346
Mass: 82383
Score: 58
Queries matched: 2
Query
60
139
21.
Observed
478.7743
596.8195
Mr(expt)
955.5340
1191.6244
Mr(calc)
955.5338
1191.6248
0.21
0
23
4.1
1
-0.26
1
36
0.37
1
Peptide
K.VVQGEPSLK.V
R.GLIKEQYEGR.L
gi|157164710
Mass: 19698
Score: 58
Queries matched: 2
molybdenum cofactor synthesis domain-containing protein [Campylobacter concisus 13826]
Query
83
195
22.
Observed
503.2729
731.8401
Mr(expt)
1004.5312
1461.6656
Mr(calc)
1004.5325
1461.6657
-1.20
0
30
2.1
1
-0.04
0
28
2
1
gi|19168448
Mass: 16787
Score: 57
Queries matched: 1
autoinducer-2 synthase; LuxS [Campylobacter coli]
Peptide
K.IGILTMSDR.A
R.DVTPEATEAVCEK.M + Acrylamide (C)
emPAI: 0.20
Query
126
Observed
568.7828
Mr(expt)
1135.5510
Mr(calc)
1135.5510
0.07
0
57 0.0031
1
Peptide
K.GDDISVFDLR.F
gi|22795066
Mass: 16774
Score: 57
Queries matched: 1
autoinducer-2 synthase [Campylobacter jejuni]
12/9/2009
Page 7 of 10
gi|57168077
Mass: 18300
Score: 57
Queries matched: 1
autoinducer-2 production protein LuxS [Campylobacter coli RM2228]
gi|57238070
Mass: 18259
Score: 57
Queries matched: 1
S-ribosylhomocysteinase [Campylobacter jejuni RM1221]
gi|86150303
Mass: 18182
Score: 57
Queries matched: 1
autoinducer-2 production protein LuxS [Campylobacter jejuni subsp. jejuni CF93-6]
gi|86150786
Mass: 18307
Score: 57
Queries matched: 1
autoinducer-2 production protein LuxS [Campylobacter jejuni subsp. jejuni 260.94]
Mass: 18246
Score: 57
Queries matched: 1
gi|86152712
S-Ribosylhomocysteinase (LuxS) [Campylobacter jejuni subsp. jejuni HB93-13]
Mass: 18197
Score: 57
Queries matched: 1
gi|88596025
autoinducer-2 production protein LuxS [Campylobacter jejuni subsp. jejuni 84-25]
gi|121613162
Mass: 18245
Score: 57
Queries matched: 1
S-ribosylhomocysteinase [Campylobacter jejuni subsp. jejuni 81-176]
gi|148926201
Mass: 18200
Score: 57
Queries matched: 1
hypothetical protein Cj8486_1236 [Campylobacter jejuni subsp. jejuni CG8486]
gi|153951890
Mass: 18295
Score: 57
Queries matched: 1
S-ribosylhomocysteinase [Campylobacter jejuni subsp. doylei 269.97]
gi|157165538
Mass: 19040
Score: 57
Queries matched: 1
S-ribosylhomocysteinase [Campylobacter concisus 13826]
gi|223040503
Mass: 19242
Score: 57
Queries matched: 1
S-ribosylhomocysteinase LuxS [Campylobacter rectus RM3267]
gi|229531421
Mass: 18757
Score: 57
Queries matched: 1
LuxS protein involved in autoinducer AI2 synthesis [Sulfurospirillum deleyianum DSM 6946]
gi|255323088
Mass: 19023
Score: 57
Queries matched: 1
S-ribosylhomocysteinase LuxS [Campylobacter showae RM3277]
23.
gi|256811403
Mass: 193229
Score: 56
Queries matched: 1
DNA-directed DNA polymerase [Methanocaldococcus fervens AG86]
emPAI: 0.02
Query
88
24.
Observed
507.2379
Mr(expt)
1012.4612
Mr(calc)
1012.4614
-0.15
0
56 0.0022
1
Peptide
R.NYDFDIAR.V
gi|123506
Mass: 8353
Score: 55
Queries matched: 1
emPAI: 0.42
Query
68
Observed
492.3032
Mr(expt)
982.5918
Mr(calc)
982.5923
-0.49
0
55 0.0011
1
Peptide
R.ALGILNLNR.N
gi|5019730
Mass: 12474
Score: 55
Queries matched: 1
Mass: 12343
Score: 55
Queries matched: 1
gi|76782253
25.
gi|157165403
Mass: 22036
Score: 55
Queries matched: 1
emPAI: 0.15
translation initiation factor IF-3 [Campylobacter concisus 13826]
Query
159
26.
Observed
622.8459
Mr(expt)
1243.6772
Mr(calc)
1243.6772
0.04
0
55 0.0035
1
gi|157164523
Mass: 21487
Score: 49
Queries matched: 1
putative outer membrane protein [Campylobacter concisus 13826]
Peptide
R.AGSGEVGINLLSK.F
emPAI: 0.16
Query
89
Observed
507.2380
Mr(expt)
1012.4614
Mr(calc)
1012.4614
0.04
0
49
0.015
1
Peptide
K.AGYDFDVAR.V
Query
197
143
92
26
Observed
739.8904
606.3404
508.7722
422.7475
Mr(expt)
1477.7662
1210.6662
1015.5298
843.4804
Mr(calc)
1477.7664
1210.6670
1015.5298
843.4814
ppm Miss Score
-0.12
0
46
-0.61
0
44
0.06
0
42
-1.11
0
42
142
42
39
25
44
35
57
130
220
95
602.8437
439.7524
435.7739
422.2577
442.2838
428.7662
475.7506
579.2988
834.9253
510.7715
1203.6728
877.4902
869.5332
842.5008
882.5530
855.5178
949.4866
1156.5830
1667.8360
1019.5284
1203.6724
877.4909
869.5334
842.5014
882.5538
855.5178
949.4869
1156.5836
1667.8366
1019.5321
0.38
-0.78
-0.23
-0.68
-0.89
0.05
-0.23
-0.52
-0.34
-3.62
0
0
0
0
0
0
0
0
0
0
40
38
37
36
35
34
34
34
33
32
Expect Rank
0.029
1
0.026
1
0.11
1
0.23
1
0.075
0.18
0.25
0.047
0.097
0.49
0.9
0.39
0.71
1.1
1
1
1
1
1
1
1
1
1
1
Peptide
STPVDILYSQVEK
QITVNDLPVGR
GESIIEAAAR
AITEALAR
HAVINDLPLGR
IFGVVDTK
VVSLSIPR
VGWLIAGK
VAIAATLPK
LATVVTPR
DAAIIEYR
QGVDADINGLR
IIPSAEDPSQDIVER
AIMVGTVGEK + Oxidation (M)
12/9/2009
Page 8 of 10
215
62
138
24
1
132
46
5
15
181
818.3917
481.2768
596.3180
421.7711
353.7156
581.7906
448.7182
365.2287
410.7244
681.3357
1634.7688
960.5390
1190.6214
841.5276
705.4166
1161.5666
895.4218
728.4428
819.4342
1360.6568
1634.7675
960.5393
1190.6217
841.5273
705.4174
1161.5675
895.4222
728.4432
819.4346
1360.6583
0.81
-0.24
-0.18
0.42
-1.01
-0.70
-0.37
-0.51
-0.47
-1.04
1
0
0
0
0
0
0
0
0
1
32
32
31
31
30
30
30
29
28
28
0.61
1.2
0.67
0.44
1
1.5
1.2
2.9
1.9
1.9
1
1
1
1
1
1
1
1
1
1
TPEVDDEALEKFDK
VSPAVFVSR
LTITVAPK
YVVGIR
STIYCPR + Carbamidomethyl (C)
ASTIPIK
MLCLAAK + Acrylamide (C)
SGSDEVQVKQER
210
191
185
51
52
102
109
34
8
12
211
174
67
180
783.8735
713.8467
695.8178
465.7663
467.2752
516.7874
534.2764
428.7660
395.2393
403.7113
783.8738
641.3157
490.2839
661.3347
1565.7324
1425.6788
1389.6210
929.5180
932.5358
1031.5602
1066.5382
855.5174
788.4640
805.4080
1565.7330
1280.6168
978.5532
1320.6548
1565.7283
1425.6776
1389.6259
929.5182
932.5365
1031.5611
1066.5407
855.5178
788.4644
805.4082
1565.7364
1280.6183
978.5538
1320.6561
2.67
0.88
-3.51
-0.15
-0.67
-0.85
-2.31
-0.42
-0.41
-0.25
-2.13
-1.15
-0.61
-0.97
1
0
1
0
0
0
0
0
0
1
1
0
0
0
28
27
26
26
26
26
26
25
25
25
24
24
24
24
1.6
1.9
1.3
4.4
2.2
2.6
3.5
4
2
2.9
3.2
5.5
4.1
6.6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
MAFPEKSEEEIEK
DFTPELQASYQK
DEEEIRDEVEK
ITEITPTR
LIVASCLSK
VGSGSASLGGIK
IFSSGINSSR
LATVVTPR
IVTVETK
FARQGEV
CLPDMMRQIVSNK + 2 Oxidation (M)
FCTTEGIANLR + Carbamidomethyl (C)
FAISVPFAK
YSEDLQPQTLK
134
19
29
84
20
172
184
91
179
235
96
66
86
37
125
216
148
112
27
218
100
106
115
40
79
145
50
105
584.7725
417.7321
425.7299
503.7635
418.7219
639.3038
691.3294
508.7484
660.3185
655.6515
511.2329
488.2790
505.8156
432.7492
565.7698
547.3171
613.8242
539.7983
423.7393
825.9370
516.3029
526.2639
544.3170
436.7747
500.2480
612.7818
465.7661
523.7776
1167.5304
833.4496
849.4452
1005.5124
835.4292
1276.5930
1380.6442
1015.4822
1318.6224
1963.9327
1020.4512
974.5434
1009.6166
863.4838
1129.5250
1638.9295
1225.6338
1077.5820
845.4640
1649.8594
1030.5912
1050.5132
1086.6194
871.5348
998.4814
1223.5490
929.5176
1045.5406
1167.5277
833.4503
849.4452
1005.5165
835.4296
1276.5904
1380.6442
1015.4822
1318.6193
1963.9309
1020.4546
974.5437
1009.6172
863.4865
1129.5251
1638.9305
1225.6343
1077.5818
845.4647
1649.8624
1030.5910
1050.5128
1086.6186
871.5378
998.4821
1223.5492
929.5182
1045.5404
2.32
-0.78
0.05
-3.99
-0.38
2.09
0.01
0.07
2.36
0.91
-3.29
-0.23
-0.55
-3.07
-0.05
-0.62
-0.34
0.19
-0.78
-1.81
0.22
0.44
0.80
-3.44
-0.69
-0.16
-0.60
0.26
0
0
0
1
0
0
0
0
0
1
0
0
0
0
0
1
0
0
0
1
0
0
1
1
0
0
0
0
23
23
23
23
22
22
21
21
21
21
20
20
20
20
19
19
19
19
19
18
17
17
17
17
17
17
17
16
4.2
5.8
6.7
9.4
7
6.4
9.9
5.4
8.5
9.1
7.9
20
2
19
7
4.3
12
18
26
15
9.9
26
18
10
14
15
40
33
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
CSHCGAVHLGK + Carbamidomethyl (C)
MLVCGIK + Acrylamide (C)
MLAMQLK + Oxidation (M)
MISDKVDAK
MLLVCNK + Oxidation (M)
DLETSIATMQEK + Oxidation (M)
TPELEENGK
DFAEYAHLEPK
AAEGCGEYNKDAIQSLPK + Acrylamide (C)
WISTVELK
VTGIPTPVVK
VAEPVPPR
EQLAEIENGQ
KVPQVSTPTLVEVSR
DGYLIANIYGK
LGSIIEFSGR
EGFPVGIK
AILYSINGGVDEKSGK
VLDELTLTK
LRVDPVNFK
ELKGILSL
FHIDNPEK
CVEAGADDFIK + Carbamidomethyl (C)
DVEVVLTR
LSSPATLDSR
41
69
157
242
201
168
234
121
98
221
212
55
108
219
437.7536
493.7791
621.3077
708.3395
755.3769
630.2967
650.9795
560.8032
513.7880
834.9275
794.8641
475.7145
533.2645
550.9613
873.4926
985.5436
1240.6008
2121.9967
1508.7392
1258.5788
1949.9167
1119.5918
1025.5614
1667.8404
1587.7136
949.4144
1064.5144
1649.8621
873.4920
985.5444
1240.6009
2121.9962
1508.7358
1258.5837
1949.9153
1119.5958
1025.5618
1667.8413
1587.7174
949.4175
1064.5138
1649.8624
0.79
-0.77
-0.05
0.23
2.29
-3.83
0.73
-3.50
-0.30
-0.53
-2.35
-3.19
0.59
-0.22
0
0
1
1
1
1
1
1
1
0
0
0
0
1
16
16
16
16
16
16
16
16
16
16
15
15
15
15
60
26
21
24
32
17
24
45
17
36
16
24
46
32
1
1
1
1
1
1
1
1
1
1
1
1
1
1
LSVALSER
IVDVGIENK
YLEKMVEEGK + Oxidation (M)
ECIEKDPGAFEEEAMIIK + Acrylamide (C)
QEENELSELYKK
MGGERGGAGAGSPR
AAEGCGEYNKDAIQSLPK + Carbamidomethyl (C)
KIELMETTR
ASRAAPSAPAK
TGNAGPRPACGVIGLTN + Acrylamide (C)
YIHHITCGDCLEK + Carbamidomethyl (C)
NEADQMVK + Oxidation (M)
AAGFNLETDK
LNIKTTDDDSLFLR
9
123
56
149
71
200
161
231
85
396.2182
563.7853
475.7287
614.3196
494.7198
503.9202
625.3306
900.4889
505.2690
790.4218
1125.5560
949.4428
1226.6246
987.4250
1508.7388
1248.6466
1798.9632
1008.5234
790.4225
1125.5601
949.4426
1226.6255
987.4266
1508.7406
1248.6462
1798.9651
1008.5240
-0.85
-3.56
0.23
-0.70
-1.58
-1.19
0.33
-1.05
-0.53
0
0
1
1
1
0
0
0
1
15
15
15
15
14
14
14
14
13
16
35
24
32
8.5
52
56
35
45
1
1
1
1
1
1
1
1
1
FGILTSGP
LLECLGHGER
KSDIDMEI
GADDTKTLAHAK
CPMDKHNK + Oxidation (M)
QLPHVQDMIDTGR
EVNLDPNPPVR
ETLASYGRL
12/9/2009
Page 9 of 10
193
124
61
53
233
32
58
190
38
97
73
722.3688
563.7854
480.8009
467.7306
970.9719
427.2392
478.2533
713.3224
435.7738
512.2404
496.7978
1442.7230
1125.5562
959.5872
933.4466
1939.9292
852.4638
954.4920
1424.6302
869.5330
1022.4662
991.5810
1442.7266
1125.5601
959.5838
933.4477
1939.9237
852.4606
954.4957
1424.6276
869.5334
1022.4703
991.5815
-2.48
-3.40
3.62
-1.15
2.86
3.81
-3.79
1.88
-0.46
-3.92
-0.42
0
1
1
0
0
1
0
1
0
0
1
13
13
12
12
12
12
12
12
12
12
12
55
68
9.9
64
64
21
69
44
83
85
25
1
1
1
1
1
1
1
1
1
1
1
DHLVQGYTLNQR
DVIHPDMRK + Oxidation (M)
VVCAVLKTK
AFDNCILTIEAYDPDIK
AIGKAWHA
LIMAGGHEK
DDVNVEKMMQGK + 2 Oxidation (M)
VVSLSIPR
TSMGDQIGSK
KTGAIVGHPL
99
246
206
144
129
178
199
160
146
140
173
244
208
230
514.7538
1088.0150
769.3247
610.7980
575.7672
655.3226
752.8674
623.2894
613.3101
598.3436
640.8824
713.6710
777.8674
897.9115
1027.4930
2174.0154
1536.6348
1219.5814
1149.5198
1308.6306
1503.7202
1244.5642
1224.6056
1194.6726
1279.7502
2137.9912
1553.7202
1793.8084
1027.4934
2174.0235
1536.6401
1219.5808
1149.5158
1308.6310
1503.7214
1244.5606
1224.6035
1194.6720
1279.7500
2137.9871
1553.7177
1793.8036
-0.34
-3.72
-3.39
0.52
3.50
-0.27
-0.75
2.91
1.75
0.53
0.21
1.89
1.61
2.70
0
0
0
0
0
1
0
1
0
0
1
0
1
1
12
11
11
11
11
11
11
11
11
10
10
10
10
9
34
63
14
61
56
79
84
50
73
60
22
94
1.1e+002
1.3e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
AGPNEEAAAAK
LTQDTLQSSEQMMVVFSSK + Oxidation (M)
GNTQDETGSQSNSGR
WQDAGFLPMR
MPAVANDGTMK + Oxidation (M)
DAGFRTTELDGK
GSSNSGGSSHRGR
GAMWYMPLIK + Oxidation (M)
AAAAELAAAAPLR
VVQGEPSLKVPK
MDSGPTIVEVSNSNTCDLLK + Oxidation (M)
MSMIPASSSQSEKR + Oxidation (M)
141
247
186
176
228
209
236
222
120
225
232
214
117
226
49
165
248
175
72
170
240
11
33
167
136
203
253
171
602.7928
725.6796
695.8464
642.3402
596.6121
778.3572
665.6370
842.8998
556.3164
883.8951
645.6776
811.4440
550.2449
886.4170
465.7192
627.2773
731.0103
641.8484
496.7209
635.2742
696.6466
399.1930
428.2614
629.3282
589.3427
504.2632
1169.8290
635.7662
1203.5710
2174.0170
1389.6782
1282.6658
1786.8145
1554.6998
1993.8892
1683.7850
1110.6182
1765.7756
1934.0110
1620.8734
1098.4752
1770.8194
929.4238
1252.5400
2190.0091
1281.6822
991.4272
1268.5338
2086.9180
796.3714
854.5082
1256.6418
1176.6708
1509.7678
3506.4652
1269.5178
1203.5706
2174.0248
1389.6736
1282.6631
1786.8136
1554.7018
1993.8815
1683.7886
1110.6147
1765.7802
1934.0108
1620.8696
1098.4764
1770.8173
929.4211
1252.5403
2190.0052
1281.6829
991.4256
1268.5352
2086.9147
796.3715
854.5086
1256.6435
1176.6754
1509.7708
3506.4615
1269.5152
0.33
-3.61
3.37
2.11
0.51
-1.24
3.85
-2.12
3.15
-2.57
0.06
2.39
-1.04
1.22
2.93
-0.16
1.79
-0.54
1.71
-1.06
1.57
-0.09
-0.44
-1.30
-3.87
-2.03
1.04
2.12
0
1
1
0
0
1
1
1
0
1
1
1
0
0
0
0
0
1
1
0
1
0
1
0
1
0
1
0
9
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
7
7
7
7
7
7
7
7
6
1.3e+002
1.1e+002
1.6e+002
1.2e+002
1e+002
98
81
1.5e+002
1.1e+002
91
1.5e+002
98
43
1.4e+002
62
93
1.4e+002
1.5e+002
56
65
90
1e+002
85
1.6e+002
1e+002
2e+002
32
49
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
VYVQCVNHDK
AHANCFIANSLACEVEIKQ + 2 Carbamidomethyl (C)
TIIIEDNRSTDN
MTPADTFLLFK
HVHHQDQGLDSFPDR
MEVCDKVTNGAASSK + Oxidation (M)
MFGALGRCFEEDPFFR + Carbamidomethyl (C); Oxidation (M)
DIARSMGISFDQSNK + Oxidation (M)
FMLVIVFDK
WECVSGSGTKNSNQNR
EKHLNVPANTEELAELK
ALKQQVHQLEASNR
GYQTMNNQK + Oxidation (M)
SASQSLHDSFDEVPPR
NQMVCHK + Acrylamide (C)
MMAASCPPFNK + Carbamidomethyl (C)
QWAALMTGDETYAGSLSFSR
LRIFNYSNQK
FCRSVYM + Acrylamide (C); Oxidation (M)
MMVRPCSFDP + Acrylamide (C); Oxidation (M)
NCIDATGTNSTSKVSEEMK + Carbamidomethyl (C); Oxidation (M)
IDYSSGR
ARGLSVPR
GGGPDGMSPILIK + Oxidation (M)
SIKYLDTIPK
TMASLQNTSTILSK + Oxidation (M)
MCEAASECSGDSNESMEEEPNGTSTIVCRVTIK
MMNCSGENIAK + Carbamidomethyl (C); Oxidation (M)
243
213
229
36
87
227
118
207
224
251
252
47
43
30
713.6705
810.8334
894.4148
428.7785
505.8158
591.2808
553.2629
777.8377
849.4448
905.0792
1164.5000
450.2745
442.2763
425.7608
2137.9897
1619.6522
1786.8150
855.5424
1009.6170
1770.8206
1104.5112
1553.6608
1696.8750
2712.2158
3490.4782
898.5344
882.5380
849.5070
2137.9950
1619.6555
1786.8209
855.5430
1009.6172
1770.8215
1104.5130
1553.6602
1696.8719
2712.2151
3490.4837
898.5348
882.5399
849.5072
-2.48
-2.04
-3.30
-0.59
-0.11
-0.55
-1.57
0.39
1.86
0.26
-1.57
-0.42
-2.12
-0.20
0
1
0
0
0
0
0
0
1
0
1
1
1
1
6
6
6
6
6
5
5
5
5
4
4
4
3
3
2.3e+002
32
2e+002
83
50
2.4e+002
2.1e+002
1.3e+002
3.3e+002
2.1e+002
72
5.8e+002
1.2e+002
2.9e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
TQINPISSTQAFNAESECK + Acrylamide (C)
SQYCSRDETMGSEK
WWSAHTGTDQMQIAR
LLVLVDGK
AEVALPLAVK
MGFGLGCAGAMLQSVTR + Carbamidomethyl (C); Oxidation (M)
MLMVHQSML + Oxidation (M)
QCLNPDCIYESR + 2 Carbamidomethyl (C)
QRVSQYYGIPMAIR + Oxidation (M)
FEQASESEPTTVSYEIAGNSPNAER
LAPFQLCLLCKCCAGAATTTCATMSCCFGIDCR + Carbamidomethyl (C); Oxidation (M)
GGKNALLAR
AVRATPLR
RYIGLTK
249
128
166
31
241
17
223
54
2
1096.0130
574.2832
627.7689
425.7608
1044.4680
414.7615
843.3922
472.2876
357.6367
2190.0114
1146.5518
1253.5232
849.5070
2086.9214
827.5084
1684.7698
942.5606
713.2588
2190.0038
1146.5517
1253.5256
849.5072
2086.9169
827.5116
1684.7663
942.5610
3.49
0.14
-1.87
-0.24
2.18
-3.86
2.12
-0.41
0
0
1
1
1
0
0
1
3
3
3
2
2
1
0
0
3.9e+002
5.4e+002
1.9e+002
3.8e+002
3.4e+002
5.3e+002
6.2e+002
9.8e+002
1
1
1
1
1
1
1
1
WPEIVDEALMTEPTEAESK + Oxidation (M)
DDIDVLTNSR
MKXDCLAXPVR
KTTFVVR
MGPSSACLQCGFQVARSCR + Acrylamide (C); Oxidation (M)
ILVPSATK
ILYYMSMEFLMGR + 2 Oxidation (M)
GIILSERR
12/9/2009
3
4
6
10
13
48
70
82
101
137
163
357.6370
362.2220
384.2352
398.2510
406.2484
458.7878
494.3007
502.8143
516.3137
589.3430
625.9136
Page 10 of 10
713.2594
722.4294
766.4558
794.4874
810.4822
915.5610
986.5868
1003.6140
1030.6128
1176.6714
1249.8126
Search Parameters
Type of search
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
MS/MS Ion Search
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
253
12/9/2009
Page 1 of 22
User
Email
Search title
MS data file
Database
Timestamp
Protein hits
:
:
:
:
:
:
:
LTQFT Daemon
D:\Data\Mark\18_7-09\N_18_7_09_7.RAW
9 Dec 2009 at 04:07:08 GMT
gi|158605017 extracellular tungstate binding protein [Campylobacter concisus 13826]
gi|157165374 DNA primase [Campylobacter concisus 13826]
gi|157164012 2-cys peroxiredoxin BAS1, (Thiol-specific antioxidant protein) [Campylobacter concisus 13826]
gi|157165109 L-asparaginase [Campylobacter concisus 13826]
gi|157164888 solute-binding family 1 protein [Campylobacter concisus 13826]
gi|1351907
gi|157165165 enoyl-(acyl carrier protein) reductase [Campylobacter concisus 13826]
gi|194676990 PREDICTED: similar to endopin 2B [Bos taurus]
gi|157165622 integrase/recombinase [Campylobacter concisus 13826]
gi|157165684 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase [Campylobacter concisus 13826]
gi|6320157
gi|122614
gi|157164251 holo-[acyl-carrier-protein] synthase [Campylobacter concisus 13826]
gi|1942778
gi|122411
gi|223570
dismutase,Mn superoxide
gi|225632
casein alphaS1
gi|291474
gi|157164741 adenylate kinase [Campylobacter concisus 13826]
gi|157164768 molybdate ABC transporter, periplasmic molybdate-binding protein [Campylobacter concisus 13826]
gi|136429
gi|7331218
gi|157164710 molybdenum cofactor synthesis domain-containing protein [Campylobacter concisus 13826]
gi|157164883 enolase (2-phosphoglycerate dehydratase; 2-phospho-D-glycerate hydro-lyase) [Campylobacter concisus 13826]
gi|157164579 cysteine synthase A [Campylobacter concisus 13826]
gi|157164816 surface antigen, CjaA [Campylobacter concisus 13826]
gi|27807517 serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 6 [Bos taurus]
gi|34558074 fumarate hydratase B, BETA subunit [Wolinella succinogenes DSM 1740]
gi|417185
RecName: Full=Leukocyte elastase inhibitor; Short=LEI; AltName: Full=Leukocyte neutral proteinase inhibitor; Short=LNPI; Alt
gi|494583
gi|194038353 PREDICTED: similar to alpha-1-antichymotrypsin 2 [Sus scrofa]
gi|154175458 ProP protein [Campylobacter curvus 525.92]
gi|157164497 fumarate hydratase [Campylobacter concisus 13826]
gi|157164023 carbon-nitrogen family hydrolase [Campylobacter concisus 13826]
gi|157165597 CRISPR-associated protein Cas2 [Campylobacter concisus 13826]
gi|3928489
gi|416751
RecName: Full=Alpha-S2-casein; Short=Alpha-S2-CN; Flags: Precursor
gi|119914036 PREDICTED: similar to Endopin 1b isoform 1 [Bos taurus]
gi|3914346
gi|47523844 serine proteinase inhibitor, clade A, member 1 [Sus scrofa]
gi|291464
gi|291462
gi|157165672 curli production assembly/transport component CsgG subfamily protein [Campylobacter concisus 13826]
gi|438069
thiol-specific antioxidant protein [Homo sapiens]
gi|158604975 D-methionine-binding lipoprotein MetQ [Campylobacter concisus 13826]
gi|165945
gi|27807521 prostaglandin D2 synthase, brain [Bos taurus]
gi|157163886 thiol peroxidase [Campylobacter concisus 13826]
gi|157165161 triosephosphate isomerase [Campylobacter concisus 13826]
gi|157165400 nitroreductase family protein [Campylobacter concisus 13826]
gi|28317
gi|149716548 PREDICTED: similar to Apolipoprotein A-I precursor (Apo-AI) (ApoA-I) [Equus caballus]
gi|154173631 periplasmic nitrate reductase, diheme cytochrome c subunit [Campylobacter curvus 525.92]
gi|149925600 probable two-component sensor histidine kinase [Limnobacter sp. MED105]
100
0
0.00 %
174
0
0.00 %
12/9/2009
Page 2 of 22
Format As
Standard scoring
Show sub-sets 0
Require bold red
Show pop-ups
Select All
1.
Help
Peptide Summary
Select None
MudPIT scoring
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Search Selected
Error tolerant
Archive Report
gi|158605017
Mass: 30046
Score: 887
Queries matched: 41
emPAI: 3.34
extracellular tungstate binding protein [Campylobacter concisus 13826]
2.
Query
17
35
36
46
63
117
146
147
167
168
183
184
217
239
377
415
427
428
452
578
579
580
582
585
586
594
595
628
630
Observed
363.6946
398.2033
398.2034
404.7317
421.7346
460.2219
485.2611
485.2612
496.7921
496.7922
504.2738
504.2739
518.2405
537.8006
633.3478
654.3455
657.8378
657.8381
679.3592
799.8973
799.8979
799.8981
534.2944
800.9381
800.9387
807.8953
807.8954
563.3174
563.3176
Mr(expt)
725.3746
794.3920
794.3922
807.4488
841.4546
918.4292
968.5076
968.5078
991.5696
991.5698
1006.5330
1006.5332
1034.4664
1073.5866
1264.6810
1306.6764
1313.6610
1313.6616
1356.7038
1597.7800
1597.7812
1597.7816
1599.8614
1599.8616
1599.8628
1613.7760
1613.7762
1686.9304
1686.9310
Mr(calc)
725.3748
794.3923
794.3923
807.4490
841.4545
918.4294
968.5080
968.5080
991.5702
991.5702
1006.5335
1006.5335
1034.4669
1073.5869
1264.6815
1306.6768
1313.6615
1313.6615
1356.7038
1597.7810
1597.7810
1597.7810
1599.8620
1599.8620
1599.8620
1613.7759
1613.7759
1686.9305
1686.9305
ppm Miss Score
Expect Rank
-0.25
0
29
0.2
1
-0.30
0
30
1.6
1
-0.05
0
(20)
15
2
-0.23
1
27
2.9
2
0.16
0
23
3.1
1
-0.18
0
23
3.4
2
-0.33
1
(21)
9.6
1
-0.12
1
23
3.8
1
-0.56
1
(22)
5.8
3
-0.36
1
28
1.5
1
-0.44
1
43
0.069
1
-0.24
1
(41)
0.095
1
-0.41
0
33
0.47
1
-0.28
0
39
0.23
1
-0.40
0
42
0.056
1
-0.31
1
50
0.012
1
-0.37
1
(40)
0.1
1
0.09
1
46
0.026
1
0.05
1
36
0.3
1
-0.61
0
(36)
0.3
1
0.14
0
(77) 1.8e-005
1
0.39
0
(74) 3.8e-005
1
-0.42
0
(28)
1.6
1
-0.25
0
58
0.0016
1
0.50
0
(30)
0.83
1
0.07
0
(50)
0.01
1
0.19
0
85 3.4e-006
1
-0.06
1
(25)
1.7
1
0.29
1
(26)
1.3
1
Peptide
K.FIGDFK.L
K.GVTFTDR.G
K.GVTFTDR.G
K.SIADKFK.G
K.IAGEVPEK.D
K.TDIENAEK.F
K.DGIKFFSR.G
K.DGIKFFSR.G
K.QSFELIKK.D
K.QSFELIKK.D
K.VEKEFVEK.G
K.VEKEFVEK.G
K.WATSEEGQK.F
K.WTAVGTGAALK.L
K.NFYSLIAVNPK.H
K.TDIENAEKFIK.W
R.GTYIKYEANQK.G
R.GTYIKYEANQK.G
K.GVTFTDRGTYIK.Y
R.NAVMYNDFVVIADK.S
R.NAVMYNDFVVIADK.S
R.NAVMYNDFVVIADK.S
K.LLDKPLFTPDANTR.K
K.LLDKPLFTPDANTR.K
K.LLDKPLFTPDANTR.K
R.NAVMYNDFVVIADK.S + Oxidation (M)
K.TGVDIKWTAVGTGAALK.L
631
632
651
667
671
677
678
679
680
844.4733
844.4735
871.9232
890.9303
898.9275
907.9402
605.6296
907.9409
605.6300
1686.9320
1686.9324
1741.8318
1779.8460
1795.8404
1813.8658
1813.8670
1813.8672
1813.8682
1686.9305
1686.9305
1741.8311
1779.8461
1795.8410
1813.8669
1813.8669
1813.8669
1813.8669
0.93
1.17
0.40
-0.05
-0.33
-0.57
0.05
0.20
0.71
1
1
1
0
0
0
0
0
0
(36)
44
56
47
(24)
(41)
56
(34)
(34)
0.14
0.021
0.0024
0.02
4.2
0.094
0.0025
0.47
0.42
1
1
1
1
1
1
1
2
1
K.WATSEEGQKFIGDFK.L
K.GHPEMVIINEGDNDLK.N
K.GHPEMVIINEGDNDLK.N +
K.LGEDCNADILFVHSPK.V +
685
686
793
914.9475
610.3012
1090.5200
1827.8804
1827.8818
3268.5382
1827.8825
1827.8825
3268.5380
-1.14
-0.41
0.05
0
0
1
(56)
(37)
67
0.0031
0.22
0.00016
1
2
1
K.LGEDCNADILFVHSPK.V + Acrylamide (C)
K.IAGEVPEKDSWYMQTGQGMLATINAAAEQK.G + 2 Oxidation (M)
gi|157165374
Mass: 33156
Score: 461
DNA primase [Campylobacter concisus 13826]
Queries matched: 13
Oxidation (M)
Carbamidomethyl
Carbamidomethyl
Carbamidomethyl
Carbamidomethyl
(C)
(C)
(C)
(C)
emPAI: 0.95
12/9/2009
Query
12
307
339
474
546
618
619
706
732
740
751
752
753
3.
Observed
359.2285
582.7859
614.2982
692.8331
756.8805
559.2689
838.4000
970.4807
1005.9920
1034.5290
1070.0390
713.6955
713.6957
Mr(expt)
716.4424
1163.5572
1226.5818
1383.6516
1511.7464
1674.7849
1674.7854
1938.9468
2009.9694
2067.0434
2138.0634
2138.0647
2138.0653
Mr(calc)
716.4432
1163.5571
1226.5819
1383.6518
1511.7468
1674.7850
1674.7850
1938.9469
2009.9694
2067.0419
2138.0644
2138.0644
2138.0644
Page 3 of 22
ppm Miss Score
Expect Rank
-1.10
0
25
5.4
1
0.14
1
37
0.23
1
-0.04
0
27
2.1
1
-0.11
0
94 3.6e-007
1
-0.20
1
41
0.097
1
-0.05
0
(26)
2.5
1
0.29
0
44
0.037
1
-0.04
0
47
0.026
1
0.01
0
69
0.00015
1
0.76
1
33
0.54
1
-0.44
1
44
0.053
1
0.13
1
(27)
2.5
1
0.41
1
(22)
8.7
1
Peptide
K.AVLVTSK.D
K.FDGAKDENLR.S
K.AILDNFDEYK.T
K.DSGLTSDLSSFAGK.K
K.DSGLTSDLSSFAGKK.Y
K.GSYDGVDHDVNTIGVK.A
K.CSVQSTGGSVYNVNNVLK.K + Acrylamide (C)
K.YNVGNPGSGNEVSTLEVFK.A
K.CSVQSTGGSVYNVNNVLKK.E + Acrylamide (C)
K.KYNVGNPGSGNEVSTLEVFK.A
gi|157164012
Mass: 22011
Score: 430
Queries matched: 27
emPAI: 1.34
2-cys peroxiredoxin BAS1, (Thiol-specific antioxidant protein) [Campylobacter concisus 13826]
Query
2
104
130
201
202
256
327
328
329
389
390
391
392
393
394
395
396
409
410
417
418
419
420
519
520
776
782
4.
Observed
351.1605
445.7234
472.7270
511.2729
511.2729
550.3088
402.2311
602.8433
602.8434
426.2405
638.8575
638.8575
426.2408
426.2408
638.8576
638.8576
426.2411
646.8550
646.8552
436.5698
436.5699
654.3513
654.3514
492.2678
737.8989
802.7130
808.0449
Mr(expt)
700.3064
889.4322
943.4394
1020.5312
1020.5312
1098.6030
1203.6715
1203.6720
1203.6722
1275.6997
1275.7004
1275.7004
1275.7006
1275.7006
1275.7006
1275.7006
1275.7015
1291.6954
1291.6958
1306.6876
1306.6879
1306.6880
1306.6882
1473.7816
1473.7832
2405.1172
2421.1129
Mr(calc)
700.3068
889.4327
943.4399
1020.5314
1020.5314
1098.6033
1203.6724
1203.6724
1203.6724
1275.7009
1275.7009
1275.7009
1275.7009
1275.7009
1275.7009
1275.7009
1275.7009
1291.6958
1291.6958
1306.6881
1306.6881
1306.6881
1306.6881
1473.7827
1473.7827
2405.1169
2421.1118
-0.49
0
29
0.46
1
-0.54
0
30
1.7
1
-0.51
1
38
0.085
1
-0.15
0
(42)
0.11
1
-0.15
0
48
0.03
1
-0.24
1
25
3
1
-0.77
0
(27)
1.5
1
-0.29
0
54
0.0031
1
-0.12
0
(39)
0.092
1
-0.98
1
(24)
3.4
1
-0.38
1
(55) 0.0027
1
-0.38
1
(48)
0.013
1
-0.28
1
(28)
1.3
1
-0.28
1
(22)
4.8
1
-0.22
1
(52) 0.0047
1
-0.22
1
67 0.00017
1
0.43
1
(25)
2.4
1
-0.31
1
(42)
0.055
1
0.00
1
(27)
1.8
1
-0.42
1
(35)
0.29
1
-0.19
1
(42)
0.07
1
-0.06
1
(31)
0.79
1
0.09
1
47
0.022
1
-0.80
0
(54) 0.0057
1
0.34
0
63 0.00056
1
0.12
1
(20)
8.8
1
0.44
1
30
0.86
1
gi|157165109
Mass: 35585
Score: 392
Queries matched: 10
L-asparaginase [Campylobacter concisus 13826]
Peptide
R.YDEFK.S
R.NIDEMIR.M
R.YDEFKSR.G
R.FPIVADMTK.S
R.FPIVADMTK.S
K.ETPVNKGGIGK.V
R.HAVINDLPLGR.N
R.HAVINDLPLGR.N
R.HAVINDLPLGR.N
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S + Oxidation (M)
R.GSFLLDKDGTVR.H
R.GSFLLDKDGTVR.H
R.GSFLLDKDGTVR.H
R.GSFLLDKDGTVR.H
R.GFDVLLEDAGVALR.G
R.GFDVLLEDAGVALR.G
K.GDAGMKPSTEGVADYLSHNESKL.K.GDAGMKPSTEGVADYLSHNESKL.- + Oxidation (M)
emPAI: 0.31
Query
1
164
177
216
223
246
407
515
606
757
5.
Observed
350.2291
494.2766
501.3248
517.7689
527.3185
542.2693
644.3578
735.8647
827.4079
1089.5100
Mr(expt)
698.4436
986.5386
1000.6350
1033.5232
1052.6224
1082.5240
1286.7010
1469.7148
1652.8012
2177.0054
Mr(calc)
698.4439
986.5396
1000.6355
1033.5233
1052.6230
1082.5244
1286.7017
1469.7150
1652.8006
2177.0059
ppm Miss Score
Expect Rank
-0.40
0
45
0.048
1
-0.99
0
41
0.13
1
-0.40
0
48
0.00055
1
-0.04
0
34
0.9
1
-0.50
0
22
1.4
2
-0.35
0
31
0.52
1
-0.48
0
24
4.6
1
-0.13
0
42
0.073
1
0.39
1
84 3.8e-006
1
-0.20
0
22
5.2
1
gi|115495209
Mass: 59194
Score: 342
Queries matched: 13
Peptide
K.AGVVVVR.D
R.INELLNSGK.A
R.VLLMLALTK.T
K.IQEFFLTH.K.LIAAVPDINK.I
K.TNTTGVDTFK.S
K.SDKPVVLVGAMR.N + Oxidation (M)
K.YGFLTSDNLNAQK.A
K.TNTTGVDTFKSPNSGK.I
K.GEQISNIGSQDMNNEVWLK.L + Oxidation (M)
emPAI: 0.46
Query
20
43
103
302
314
365
Observed
367.2339
403.7114
443.7281
580.7900
587.7978
626.2782
Mr(expt)
732.4532
805.4082
885.4416
1159.5654
1173.5810
1250.5418
Mr(calc)
732.4534
805.4083
885.4419
1159.5656
1173.5812
1250.5424
-0.21
0
44
0.015
1
-0.04
0
25
2.7
1
-0.24
0
42
0.024
1
-0.09
0
54 0.0047
1
-0.12
0
(39)
0.15
1
-0.43
0
(34)
0.26
1
Peptide
R.FNLVLK.N
R.FVGDGGVR.M
R.FSVQMFK.F
12/9/2009
366
376
401
404
640
646
779
6.
626.2787
633.2866
641.2844
642.7745
856.8495
865.3624
804.6771
1250.5428
1264.5586
1280.5542
1283.5344
1711.6844
1728.7102
2411.0095
1250.5424
1264.5580
1280.5529
1283.5340
1711.6843
1728.7083
2411.0084
0.37
0.49
1.02
0.38
0.10
1.12
0.44
0
0
0
0
0
1
1
(27)
1.2
(34)
0.24
50 0.0039
38
0.044
28
0.2
25
0.71
36
0.047
gi|157164888
Mass: 27581
Score: 323
Queries matched: 9
solute-binding family 1 protein [Campylobacter concisus 13826]
1
1
1
1
1
1
1
Page 4 of 22
R.MPEDCVPTFR.C + Acrylamide (C)
R.MPEDCVPTFR.C + Acrylamide (C); Oxidation (M)
R.YCTDPATAEDK.C + Acrylamide (C)
R.STENTEDIQGCDSDK.H + Acrylamide (C)
R.YCTDPATAEDKCDR.T + 2 Acrylamide (C)
R.STENTEDIQGCDSDKHGWYR.F + Acrylamide (C)
emPAI: 0.41
Query
115
132
145
153
211
308
386
572
614
7.
Observed
455.7585
474.7626
482.7602
487.7796
515.7634
583.2598
637.3267
796.8999
833.4383
Mr(expt)
909.5024
947.5106
963.5058
973.5446
1029.5122
1164.5050
1272.6388
1591.7852
1664.8620
Mr(calc)
909.5032
947.5110
963.5059
973.5444
1029.5131
1164.5047
1272.6390
1591.7842
1664.8621
-0.81
0
28
1.3
1
-0.34
0
37
0.4
2
-0.04
0
(30)
1.6
1
0.28
1
37
0.24
2
-0.83
1
25
14
1
0.29
0
55
0.0024
1
-0.15
0
57
0.0024
1
0.65
0
62 0.00078
1
-0.02
1
23
6.8
1
Peptide
K.ERPNAPVK.I
K.SMELLISR.R
K.SMELLISR.R + Oxidation (M)
K.EGKLEAISK.S
K.SYFGKDVSK.K.DADSATSNWAK.V
R.DNIVFYDFIK.E
K.ADVVFNQVSITDER.K
K.IAASLDEKDYTAAAVK.K
gi|1351907
Mass: 69248
Score: 316
Queries matched: 10
emPAI: 0.20
Query
32
71
118
178
193
306
505
511
525
601
Observed
395.2393
424.2554
464.2502
501.7948
507.8129
582.3192
722.3240
729.3323
740.4020
547.3172
Mr(expt)
788.4640
846.4962
926.4858
1001.5750
1013.6112
1162.6238
1442.6334
1456.6500
1478.7894
1638.9298
Mr(calc)
788.4644
846.4963
926.4861
1001.5757
1013.6121
1162.6234
1442.6347
1456.6504
1478.7881
1638.9305
-0.41
0
22
4.5
2
-0.09
1
33
0.27
1
-0.30
0
28
1.3
1
-0.66
0
35
0.3
1
-0.82
0
28
0.9
1
0.41
0
52 0.0069
1
-0.90
0
(32)
0.47
1
-0.24
0
35
0.19
1
0.88
0
54 0.0038
1
-0.43
1
30
0.34
1
Peptide
K.LVTDLTK.V
R.LSQKFPK.A
K.YLYEIAR.R
K.LVNELTEFAK.T
K.YICDNQDTISSK.L + Carbamidomethyl (C)
K.YICDNQDTISSK.L + Acrylamide (C)
K.LGEYGFQNALIVR.Y
R.KVPQVSTPTLVEVSR.S
gi|30794280
Mass: 69278
Score: 316
Queries matched: 10
8.
gi|157165165
Mass: 29118
Score: 297
Queries matched: 6
emPAI: 0.72
enoyl-(acyl carrier protein) reductase [Campylobacter concisus 13826]
Query
148
163
264
435
456
533
9.
Observed
485.2899
494.2665
554.2931
669.3324
681.3564
746.4064
Mr(expt)
968.5652
986.5184
1106.5716
1336.6502
1360.6982
1490.7982
Mr(calc)
968.5655
986.5185
1106.5720
1336.6510
1360.6987
1490.7981
ppm Miss Score
Expect Rank
-0.22
0
51
0.0049
1
-0.06
0
31
0.96
1
-0.34
0
85 5.4e-006
1
-0.60
0
52
0.0058
1
-0.30
1
31
1.3
1
0.12
0
48
0.012
1
gi|194676990
Mass: 45494
Score: 295
Queries matched: 8
Peptide
R.VNAISAGPIK.T
R.YLAHDLGAK.N
K.TLAASGIGDFR.M
K.EALEGEFVNTTK.E
K.RVEPIAEEFGSK.F
K.EGGSVLTLTYLGGPK.F
emPAI: 0.23
Query
58
161
171
244
270
334
662
663
10.
Observed
417.7048
493.7975
497.8057
540.2957
557.8451
608.3032
884.4120
884.4128
Mr(expt)
833.3950
985.5804
993.5968
1078.5768
1113.6756
1214.5918
1766.8094
1766.8110
Mr(calc)
833.3953
985.5808
993.5971
1078.5771
1113.6757
1214.5931
1766.8112
1766.8112
-0.28
0
39
0.13
1
-0.34
0
51 0.0087
1
-0.26
0
43
0.014
1
-0.22
0
42
0.078
1
-0.08
1
22
2.4
1
-1.06
0
47
0.018
1
-0.97
0
51
0.007
1
-0.06
0
(35)
0.31
1
gi|157164859
Mass: 27476
Score: 287
Queries matched: 7
Peptide
K.MQDLEAK.L
R.DILSQLGIK.K
K.LAVSQVIHK.A
R.HIDELSLPR.F
R.DILSQLGIKK.I
R.FSISSDYQLR.D
K.IFTSDADFSGITNDHK.L
emPAI: 0.58
Query
86
Observed
429.7684
Mr(expt)
857.5222
Mr(calc)
857.5222
ppm Miss Score
0.05
0
23
Expect Rank
5.4
1
Peptide
K.GVELIISK.R
12/9/2009
141
325
375
589
643
648
11.
479.2555
601.3245
632.8301
803.3968
860.4528
868.4504
956.4964
1200.6344
1263.6456
1604.7790
1718.8910
1734.8862
956.4967
1200.6350
1263.6459
1604.7794
1718.8913
1734.8862
-0.28
-0.44
-0.23
-0.25
-0.13
0.04
1
0
0
0
0
0
30
1.1
39
0.15
60
0.0014
70 0.00013
65 0.00035
(36)
0.24
gi|157165622
Mass: 24092
Score: 278
Queries matched: 7
integrase/recombinase [Campylobacter concisus 13826]
1
1
1
1
1
1
Page 5 of 22
K.YFGKDISK.K.GNTELLEAINK.A
K.NGATVVVADGFSK.G
K.ADVVFNQVSINEDR.K
K.IAYTSNEPMPTAALLK.K
K.IAYTSNEPMPTAALLK.K + Oxidation (M)
emPAI: 0.68
Query
28
298
341
355
398
443
448
12.
Observed
384.7195
577.7878
614.8557
620.7955
639.3218
675.8707
678.3459
Mr(expt)
767.4244
1153.5610
1227.6968
1239.5764
1276.6290
1349.7268
1354.6772
Mr(calc)
767.4251
1153.5615
1227.6976
1239.5771
1276.6299
1349.7265
1354.6769
-0.88
0
21
1.8
1
-0.40
1
39
0.083
1
-0.59
0
21
8.9
1
-0.56
1
26
2.5
1
-0.65
1
56
0.0035
1
0.28
0
69 0.00011
1
0.27
0
46
0.028
1
Peptide
K.YLIMTK.S
K.TKGDYGEVASK.V
K.IQGVPAFVVNGK.Y
K.KWDESYDVAK.I
K.ADYEKELANPK.V
K.VFAVLIVMDEAK.G + Oxidation (M)
K.GVSLFDENSLFK.K
gi|157165684
Mass: 26183
Score: 252
Queries matched: 10
emPAI: 0.62
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase [Campylobacter concisus 13826]
Query
30
157
506
513
552
556
576
593
723
724
13.
Observed
394.7294
491.2477
723.3745
731.3719
766.4313
774.4286
799.4470
807.4447
991.5367
661.3604
Mr(expt)
787.4442
980.4808
1444.7344
1460.7292
1530.8480
1546.8426
1596.8794
1612.8748
1981.0588
1981.0594
Mr(calc)
787.4439
980.4815
1444.7344
1460.7293
1530.8480
1546.8429
1596.8797
1612.8746
1981.0592
1981.0592
0.39
0
27
4.9
1
-0.63
0
46
0.023
1
0.04
0
52 0.0068
1
-0.04
0
(42) 0.077
1
0.06
0
(32)
0.34
1
-0.15
0
37
0.13
1
-0.15
0
(23)
3
1
0.15
0
32
0.5
1
-0.20
0
56 0.0016
1
0.07
0
(35)
0.2
1
Peptide
K.QSELIAK.I
K.DGSYDALLK.K
K.INGIISAMSATPDR.L
K.INGIISAMSATPDR.L + Oxidation (M)
K.IINMSFDGLIPALK.A
K.IINMSFDGLIPALK.A + Oxidation (M)
K.VVPSEDTVPLIMGLK.V
K.VVPSEDTVPLIMGLK.V + Oxidation (M)
K.VGVQQGTIQELAANAINGAK.V
gi|6320157
Mass: 19051
Score: 243
Queries matched: 17
emPAI: 1.25
Query
4
5
18
349
350
351
352
353
354
363
Observed
353.7158
353.7158
365.2288
412.2391
617.8555
617.8555
617.8556
617.8557
617.8558
625.3301
Mr(expt)
705.4170
705.4170
728.4430
1233.6955
1233.6964
1233.6964
1233.6966
1233.6968
1233.6970
1248.6456
Mr(calc)
705.4174
705.4174
728.4432
1233.6969
1233.6969
1233.6969
1233.6969
1233.6969
1233.6969
1248.6462
-0.44
0
29
1.4
1
-0.44
0
(29)
1.4
4
-0.24
0
35
0.6
1
-1.14
0
(29)
0.67
1
-0.35
0
61 0.00041
1
-0.35
0
(42)
0.034
1
-0.19
0
(58)
0.001
1
-0.03
0
(52)
0.004
1
0.13
0
(53) 0.0032
1
-0.47
0
27
2.5
1
Peptide
K.YVVVAR.A
K.YVVVAR.A
K.QLVEIK.E
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.EVNLDPNPPVR.G
457
458
459
697
698
700
701
681.9028
681.9030
454.9378
641.3745
961.5583
641.3749
641.3749
1361.7910
1361.7914
1361.7916
1921.1017
1921.1020
1921.1029
1921.1029
1361.7918
1361.7918
1361.7918
1921.1037
1921.1037
1921.1037
1921.1037
-0.58
-0.29
-0.20
-1.05
-0.85
-0.42
-0.42
K.KIVEIPGEVPPGK.Y
K.KIVEIPGEVPPGK.Y
K.KIVEIPGEVPPGK.Y
1
1
1
1
1
1
1
(31)
46
(24)
(23)
(37)
45
(30)
0.18
0.0068
0.95
0.7
0.024
0.0045
0.13
1
1
1
1
1
1
1
gi|151941952
Mass: 19021
Score: 243
Queries matched: 17
gi|190405057
Mass: 19007
Score: 243
Queries matched: 17
14.
gi|122614
Mass: 15998
Score: 240
Queries matched: 8
emPAI: 1.62
Query
297
387
388
499
Observed
575.3405
637.8662
637.8665
713.8458
Mr(expt)
1148.6664
1273.7178
1273.7184
1425.6770
Mr(calc)
1148.6666
1273.7183
1273.7183
1425.6776
ppm Miss Score
-0.11
0
51
-0.35
0
(28)
0.12
0
49
-0.39
0
52
Expect Rank
0.0029
1
1.1
1
0.009
1
0.0072
1
Peptide
K.VVAGVANALAHK.Y
R.LLVVYPWTQR.F
R.LLVVYPWTQR.F
K.DFTPELQASYQK.V
12/9/2009
510
729
734
735
483.9368
1000.4640
672.6436
1008.4620
1448.7886
1998.9134
2014.9090
2014.9094
1448.7888
1998.9146
2014.9095
2014.9095
-0.17
-0.57
-0.26
-0.03
1
0
0
0
22
4.5
66 0.00018
(30)
0.71
(35)
0.22
1
1
1
1
Page 6 of 22
K.VVAGVANALAHKYH.R.FFDSFGDLSNPGAVMGNPK.V
gi|230480
Mass: 15970
Score: 240
Queries matched: 8
Mass: 16129
Score: 240
Queries matched: 8
gi|255683515
15.
gi|157164251
Mass: 29645
Score: 220
Queries matched: 7
emPAI: 0.11
holo-[acyl-carrier-protein] synthase [Campylobacter concisus 13826]
Query
150
203
204
248
259
512
731
16.
Observed
486.2797
511.2872
511.2873
543.8113
550.3269
729.8469
1002.0250
Mr(expt)
970.5448
1020.5598
1020.5600
1085.6080
1098.6392
1457.6792
2002.0354
Mr(calc)
970.5447
1020.5604
1020.5604
1085.6080
1098.6397
1457.6787
2002.0371
0.13
0
41
0.11
1
-0.51
1
41
0.11
1
-0.31
1
(28)
2.4
1
0.00
0
47
0.03
1
-0.39
1
31
0.8
1
0.40
1
29
0.94
1
-0.82
0
32
0.62
1
Peptide
R.IIDDLINR.K
K.KFAELASQK.S
K.KFAELASQK.S
K.TANDIIAQLK.N
R.IIDDLINRK.L
K.VSDSDLRDFYNK.N
K.AAGTVSFEQAKPEIEQAVK.M
gi|1942778
Mass: 15089
Score: 212
Queries matched: 9
emPAI: 0.84
Query
218
224
225
249
291
383
536
537
538
Observed
521.2753
529.2729
529.2729
544.3163
573.3300
634.8586
500.5790
750.3656
750.3658
Mr(expt)
1040.5360
1056.5312
1056.5312
1086.6180
1144.6454
1267.7026
1498.7152
1498.7166
1498.7170
gi|146149083
Mass:
gi|146149272
Mass:
17.
Mr(calc)
1040.5365
1056.5314
1056.5314
1086.6186
1144.6452
1267.7024
1498.7164
1498.7164
1498.7164
-0.43
0
37
0.16
1
-0.16
0
(31)
0.77
1
-0.16
0
(34)
0.42
1
-0.49
1
22
9.3
1
0.24
1
51 0.0071
1
0.21
0
60 0.0006
1
-0.85
0
(26)
2.7
1
0.13
0
43
0.047
1
0.40
0
(24)
3.4
1
15220
Score: 212
caballus]
15220
Score: 212
caballus]
Peptide
R.MFLGFPTTK.T
K.LRVDPVNFK.L
-.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEFGAEALER.M
K.VGGHAGEFGAEALER.M
K.VGGHAGEFGAEALER.M
Queries matched: 9
Queries matched: 9
gi|122411
Mass: 15236
Score: 211
Queries matched: 9
emPAI: 0.50
Query
218
224
225
249
291
383
547
548
549
Observed
521.2753
529.2729
529.2729
544.3163
573.3300
634.8586
505.9110
758.3629
758.3633
Mr(expt)
1040.5360
1056.5312
1056.5312
1086.6180
1144.6454
1267.7026
1514.7112
1514.7112
1514.7120
Mr(calc)
1040.5365
1056.5314
1056.5314
1086.6186
1144.6452
1267.7024
1514.7113
1514.7113
1514.7113
-0.43
0
37
0.16
1
-0.16
0
(31)
0.77
1
-0.16
0
(34)
0.42
1
-0.49
1
22
9.3
1
0.24
1
51 0.0071
1
0.21
0
60 0.0006
1
-0.12
0
(30)
0.73
2
-0.07
0
41
0.057
1
0.46
0
(29)
0.94
1
Peptide
R.MFLGFPTTK.T
K.LRVDPVNFK.L
M.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
gi|230479
Mass: 15105
Score: 211
Queries matched: 9
gi|230632
Mass: 15105
Score: 211
Queries matched: 9
gi|313500
Mass: 15236
Score: 211
Queries matched: 9
Mass: 15105
Score: 211
Queries matched: 9
gi|24158717
18.
gi|223570
Mass: 22716
Score: 206
Queries matched: 5
emPAI: 0.51
Query
229
283
Observed
529.8046
565.3112
Mr(expt)
1057.5946
1128.6078
Mr(calc)
1057.5954
1128.6080
ppm Miss Score
-0.68
0
36
-0.14
0
52
Expect Rank
0.18
1
0.0045
1
Peptide
K.MIAIQQNIK.F
K.AIWNVVNWK.E
12/9/2009
501
567
568
716.9009
789.4064
789.4067
1431.7872
1576.7982
1576.7988
1431.7874
1576.7984
1576.7984
-0.14
-0.12
0.26
0
0
0
49
0.01
68 0.00023
(55) 0.0042
Page 7 of 22
1
1
1
K.LAGVQGSGWAFIVK.N
K.AIDEQFGSLDELIK.L
K.AIDEQFGSLDELIK.L
gi|6321796
Mass: 25758
Score: 206
Queries matched: 5
Mitochondrial superoxide dismutase, protects cells against oxygen toxicity; phosphorylated; Sod2p [Saccharomyces cerevisiae]
Mass: 24079
Score: 206
Queries matched: 5
gi|217035334
Chain A, The Crystal Structure Of Sod2 From Saccharomyces Cerevisiae
19.
gi|225632
Mass: 24420
casein alphaS1
Score: 204
Queries matched: 6
emPAI: 0.67
Query
382
436
475
655
656
657
Observed
634.3554
669.3441
692.8689
587.3193
880.4755
880.4755
Mr(expt)
1266.6962
1336.6736
1383.7232
1758.9361
1758.9364
1758.9364
Mr(calc)
1266.6972
1336.6735
1383.7227
1758.9377
1758.9377
1758.9377
-0.74
0
55 0.0023
1
0.10
1
53 0.0054
1
0.37
0
42
0.067
1
-0.90
0
(31)
0.86
1
-0.69
0
53
0.005
1
-0.69
0
(24)
4.4
1
Peptide
R.YLGYLEQLLR.L
K.HIQKEDVPSER.Y
R.FFVAPFPEVFGK.E
K.HQGLPQEVLNENLLR.F
K.HQGLPQEVLNENLLR.F
K.HQGLPQEVLNENLLR.F
gi|30794348
Mass: 24513
Score: 204
Queries matched: 6
casein alpha s1 [Bos taurus]
Mass: 13839
Score: 204
Queries matched: 6
gi|159793187
alpha S1 casein [Bos taurus]
gi|159793191
Mass: 23598
Score: 204
Queries matched: 6
gi|159793193
Mass: 18671
Score: 204
Queries matched: 6
gi|159793197
Mass: 23473
Score: 204
Queries matched: 6
Mass: 22442
Score: 204
Queries matched: 6
gi|159793209
20.
gi|291474
Mass: 17423
Score: 203
Queries matched: 6
emPAI: 0.43
Query
213
288
301
331
522
523
Observed
516.2611
572.7686
579.7760
604.3068
738.8678
738.8679
Mr(expt)
1030.5076
1143.5226
1157.5374
1206.5990
1475.7210
1475.7212
Mr(calc)
1030.5083
1143.5230
1157.5387
1206.5993
1475.7216
1475.7216
ppm Miss Score
Expect Rank
-0.66
0
54
0.0055
1
-0.33
0
(22)
3.5
1
-1.05
0
35
0.15
1
-0.21
1
21
9.4
1
-0.40
0
(41)
0.1
1
-0.26
0
93 5.8e-007
1
Peptide
K.YAASSYLTR.T
K.SYSSVSCQVK.H + Carbamidomethyl (C)
K.SYSSVSCQVK.H + Acrylamide (C)
R.ASGVPDRFSGSK.S
K.VNDAVTTDGVQTTR.S
K.VNDAVTTDGVQTTR.S
gi|291478
Mass: 22654
Score: 203
Queries matched: 6
21.
gi|157164741
Mass: 20646
Score: 199
Queries matched: 4
adenylate kinase [Campylobacter concisus 13826]
emPAI: 0.83
22.
Query
221
294
406
Observed
524.2692
574.3090
643.8767
Mr(expt)
1046.5238
1146.6034
1285.7388
Mr(calc)
1046.5244
1146.6033
1285.7394
-0.51
0
50 0.0092
1
0.13
0
53 0.0096
1
-0.44
0
49 0.0084
1
Peptide
R.AEVASGSELGK.L
K.SNIIIDGYPR.S
K.NLFLIIGAPGSGK.T
460
682.7948
1363.5750
1363.5753
-0.15
R.GADDNNEVFNNR.M
0
47
0.0061
1
gi|157164768
Mass: 27288
Score: 191
Queries matched: 5
emPAI: 0.26
molybdate ABC transporter, periplasmic molybdate-binding protein [Campylobacter concisus 13826]
Query
19
332
479
638
639
23.
Observed
366.2185
605.8086
697.8931
856.4489
571.3018
Mr(expt)
730.4224
1209.6026
1393.7716
1710.8832
1710.8836
Mr(calc)
730.4225
1209.6030
1393.7718
1710.8828
1710.8828
-0.03
0
33
1.4
1
-0.28
0
64 0.00059
1
-0.09
0
39
0.073
1
0.25
1
56
0.0024
1
0.44
1
(21)
7.2
1
gi|136429
Mass: 24394
Score: 190
Queries matched: 10
Peptide
K.ASIEALK.N
K.AYDTGFAVAAPK.V
K.VYAQGAVAIFSIR.N
K.IYDEVEKNIVYAQK.I
K.IYDEVEKNIVYAQK.I
emPAI: 0.47
12/9/2009
Query
64
65
66
219
220
762
764
765
766
773
Observed
421.7582
421.7583
421.7583
523.2853
523.2854
737.7062
1106.0560
1106.0560
737.7067
767.0631
Mr(expt)
841.5018
841.5020
841.5020
1044.5560
1044.5562
2210.0968
2210.0974
2210.0974
2210.0983
2298.1675
Mr(calc)
841.5022
841.5022
841.5022
1044.5564
1044.5564
2210.0967
2210.0967
2210.0967
2210.0967
2298.1678
Page 8 of 22
ppm Miss Score
Expect Rank
-0.36
0
56
0.0041
1
-0.12
0
(44)
0.063
1
-0.12
0
(41)
0.12
1
-0.29
0
(30)
1.4
1
-0.10
0
37
0.35
1
0.02
0
(58)
0.002
1
0.33
0
72 7.1e-005
1
0.33
0
(61)
0.001
1
0.70
0
(50)
0.01
1
-0.14
0
25
3.3
1
Peptide
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
K.LSSPATLNSR.V
K.LSSPATLNSR.V
K.IITHPNFNGNTLDNDIMLIK.L + Oxidation (M)
gi|494360
Mass: 23458
Score: 190
Queries matched: 10
gi|999627
Mass: 8814
Score: 190
Queries matched: 10
gi|1942351
Mass: 13284
Score: 190
Queries matched: 10
gi|2914482
Mass: 23460
Score: 190
Queries matched: 10
Mass: 23457
Score: 190
Queries matched: 10
gi|3318722
Mass: 23458
Score: 190
Queries matched: 10
gi|110590762
Mass: 24157
Score: 190
Queries matched: 10
gi|157878102
gi|242253868
Mass: 25865
Score: 190
Queries matched: 10
24.
gi|7331218
Mass: 65978
Score: 190
Queries matched: 5
emPAI: 0.05
Query
151
215
414
478
521
Observed
487.2697
517.2618
651.8607
697.3694
738.3961
Mr(expt)
972.5248
1032.5090
1301.7068
1392.7242
1474.7776
Mr(calc)
972.5240
1032.5087
1301.7078
1392.7249
1474.7780
0.90
0
35
0.73
1
0.31
0
29
1.3
1
-0.75
0
63 0.00035
1
-0.45
1
27
1.9
1
-0.23
0
35
0.35
1
Peptide
K.IEISELNR.V
R.TLLEGEESR.M
R.SLDLDSIIAEVK.A
R.TNAENEFVTIKK.D
R.FLEQQNQVLQTK.W
Mass: 66027
Score: 190
Queries matched: 5
gi|11935049
gi|109096823
Mass: 65170
Score: 190
Queries matched: 5
Mass: 64332
Score: 190
Queries matched: 5
gi|109096825
gi|109096827
Mass: 62980
Score: 190
Queries matched: 5
gi|109096829
Mass: 61838
Score: 190
Queries matched: 5
gi|109096833
Mass: 61566
Score: 190
Queries matched: 5
Mass: 58846
Score: 190
Queries matched: 5
gi|109096835
gi|119395750
Mass: 65999
Score: 190
Queries matched: 5
gi|160961491
Mass: 65450
Score: 190
Queries matched: 5
25.
gi|157165413
Mass: 23563
Score: 183
Queries matched: 3
emPAI: 0.49
Query
260
342
346
26.
Observed
551.8086
615.3058
615.8562
Mr(expt)
1101.6026
1228.5970
1229.6978
Mr(calc)
1101.6030
1228.5976
1229.6979
-0.29
0
72 7e-005
1
-0.42
0
55
0.002
1
-0.06
1
55
0.002
1
Peptide
K.LEIVQTSNAK.T
K.EGLGSVEFYTK.E
K.KLEIVQTSNAK.T
gi|157165403
Mass: 22036
Score: 182
Queries matched: 4
emPAI: 0.53
Query
273
274
Observed
559.3340
559.3342
Mr(expt)
1116.6534
1116.6538
Mr(calc)
1116.6543
1116.6543
ppm Miss Score
-0.75
0
(44)
-0.39
0
60
Expect Rank
0.023
1
0.00061
1
Peptide
K.FLGALLQVEK.K
K.FLGALLQVEK.K
12/9/2009
357
681
27.
622.8456
912.4237
1243.6766
1822.8328
1243.6772
1822.8333
-0.44
-0.25
0
0
51
71
0.0073
6.7e-005
1
1
Page 9 of 22
R.AGSGEVGINLLSK.F
K.NQNIEFSLESNGNETK.I
gi|157164710
Mass: 19698
Score: 181
Queries matched: 6
emPAI: 0.88
molybdenum cofactor synthesis domain-containing protein [Campylobacter concisus 13826]
28.
Query
181
Observed
503.2733
Mr(expt)
1004.5320
Mr(calc)
1004.5325
-0.41
0
(38)
0.33
1
Peptide
K.IGILTMSDR.A
200
454
509
514
558
511.2709
679.4013
724.8322
731.8402
776.8782
1020.5272
1356.7880
1447.6498
1461.6658
1551.7418
1020.5274
1356.7878
1447.6501
1461.6657
1551.7416
-0.12
0.22
-0.15
0.09
0.14
K.IGILTMSDR.A + Oxidation (M)
R.GHALIINLPGQPK.A
R.DVTPEATEAVCEK.M + Carbamidomethyl (C)
R.DVTPEATEAVCEK.M + Acrylamide (C)
R.ASEGTYEDKSGPAIK.E
0
0
0
0
1
43
0.14
49 0.0042
(34)
0.38
56 0.0026
33
0.53
1
1
1
1
1
gi|157164883
Mass: 24731
Score: 177
Queries matched: 5
emPAI: 0.14
enolase (2-phosphoglycerate dehydratase; 2-phospho-D-glycerate hydro-lyase) [Campylobacter concisus 13826]
Query
26
125
162
241
425
29.
Observed
381.7285
468.7306
494.2538
538.7560
657.3405
Mr(expt)
761.4424
935.4466
986.4930
1075.4974
1312.6664
Mr(calc)
761.4436
935.4461
986.4933
1075.4968
1312.6663
-1.46
0
24
4.3
4
0.61
0
28
2.2
1
-0.30
0
56
0.003
1
0.61
0
24
3.3
1
0.13
1
45
0.032
1
gi|157164579
Mass: 31381
Score: 166
Queries matched: 5
cysteine synthase A [Campylobacter concisus 13826]
Peptide
R.LFNAIGK.V
K.AFAGESQAR.N
K.EAGYHAIAR.H
K.CGNEIEVQK.V + Carbamidomethyl (C)
R.NKYELYGDLAK.E
emPAI: 0.22
Query
126
128
172
289
322
30.
Observed
469.2622
471.7842
498.2650
573.2809
598.2950
Mr(expt)
936.5098
941.5538
994.5154
1144.5472
1194.5754
Mr(calc)
936.5103
941.5546
994.5157
1144.5472
1194.5768
-0.43
0
24
2.2
1
-0.78
0
22
5.7
1
-0.29
0
22
4.1
1
0.01
0
52 0.0047
1
-1.14
0
44
0.031
1
gi|157164816
Mass: 31190
Score: 150
Queries matched: 6
surface antigen, CjaA [Campylobacter concisus 13826]
Peptide
R.IAFNMITK.M
K.TIGNTPIVK.V
-.MIYDNIVK.T
K.VSNDDALNAAR.A
K.TGADEAEIYVK.L
emPAI: 0.22
Query
227
228
303
464
470
481
31.
Observed
529.7612
529.7617
580.7987
684.9087
691.3627
699.3608
Mr(expt)
1057.5078
1057.5088
1159.5828
1367.8028
1380.7108
1396.7070
Mr(calc)
1057.5080
1057.5080
1159.5833
1367.8024
1380.7112
1396.7061
-0.18
0
32
0.52
1
0.76
0
(25)
2.4
1
-0.37
0
33
0.63
1
0.32
0
41
0.022
1
-0.22
0
45
0.033
1
0.70
0
(22)
5.9
1
Peptide
K.GTTADAFFTK.N
K.GTTADAFFTK.N
K.AAASNQTDAIAK.I
K.VSLGIVSPEGAVIK.S
R.AQVVDFALPYMK.V
R.AQVVDFALPYMK.V + Oxidation (M)
gi|27807517
Mass: 42533
Score: 150
Queries matched: 4
emPAI: 0.08
serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 6 [Bos taurus]
Query
14
113
192
317
32.
Observed
362.6974
454.7453
507.7192
590.3598
Mr(expt)
723.3802
907.4760
1013.4238
1178.7050
Mr(calc)
723.3803
907.4763
1013.4236
1178.7063
-0.04
0
26
2
1
-0.30
0
34
0.7
2
0.20
0
53 0.0013
1
-1.07
0
37
0.036
1
Peptide
K.ALTYEK.F
R.DTQYLLR.T
R.ADFSGMSSGR.G
R.LVLVNAIYFK.G
gi|34558074
Mass: 19528
Score: 139
Queries matched: 5
emPAI: 0.17
fumarate hydratase B, BETA subunit [Wolinella succinogenes DSM 1740]
Query
70
516
666
694
695
Observed
422.7477
735.9169
888.9623
635.6742
953.0093
Mr(expt)
843.4808
1469.8192
1775.9100
1904.0008
1904.0040
Mr(calc)
843.4814
1469.8202
1775.9094
1904.0043
1904.0043
-0.64
0
33
1.9
1
-0.63
0
51 0.0062
1
0.39
0
27
2.5
1
-1.86
1
29
1.2
1
-0.14
1
(24)
4
1
Peptide
K.ALTEALAR.G
K.AGDNVLISGTIIAAR.D
K.YEVLAYPELGPEAVAR.L
K.KYEVLAYPELGPEAVAR.L
K.KYEVLAYPELGPEAVAR.L
gi|157164667
Mass: 19784
Score: 139
Queries matched: 5
L-cystine import ATP-binding protein TcyC [Campylobacter concisus 13826]
12/9/2009
33.
Page 10 of 22
gi|417185
Mass: 42486
Score: 133
Queries matched: 3
emPAI: 0.08
RecName: Full=Leukocyte elastase inhibitor; Short=LEI; AltName: Full=Leukocyte neutral proteinase inhibitor; Short=LNPI; AltName: Full=Serpin B1
Query
251
317
504
Observed
546.2957
590.3598
721.3690
Mr(expt)
1090.5768
1178.7050
1440.7234
Mr(calc)
1090.5771
1178.7063
1440.7249
-0.25
0
45
0.061
1
-1.07
0
37
0.036
1
-1.00
0
51 0.0095
1
Peptide
R.LGVQDLFGSR.A
K.LVLVNAIYFK.G
K.TYGAELASVDFLR.A
gi|194037951
Mass: 42544
Score: 133
Queries matched: 3
PREDICTED: similar to Leukocyte elastase inhibitor (LEI) (Serpin B1) (Leukocyte neutral proteinase inhibitor) (LNPI) [Sus scrofa]
34.
gi|157165180
Mass: 20941
Score: 125
Queries matched: 3
emPAI: 0.35
Query
73
645
702
35.
Observed
424.7653
576.3156
964.4681
Mr(expt)
847.5160
1725.9250
1926.9216
Mr(calc)
847.5167
1725.9261
1926.9224
-0.80
0
24
2.8
1
-0.65
0
33
0.38
1
-0.40
0
68 0.00022
1
Peptide
R.IISVAFAK.T
gi|494583
Mass: 15714
Score: 121
Queries matched: 4
Query
53
174
245
787
Observed
414.2703
499.8029
541.7645
905.0809
Mr(expt)
826.5260
997.5912
1081.5144
2712.2209
Mr(calc)
826.5276
997.5920
1081.5152
2712.2151
-1.92
0
28
1
1
-0.79
0
40
0.062
1
-0.74
0
30
0.86
1
2.14
0
22
3.8
1
Peptide
-.VQAVAVLK.G
K.LIGPTSVVGR.S
K.THGAPTDEVR.H
K.FEQASESEPTTVSYEIAGNSPNAER.G
gi|6322564
Mass: 15845
Score: 121
Queries matched: 4
Mass: 15680
Score: 121
Queries matched: 4
gi|6730103
gi|15826571
Mass: 15724
Score: 121
Queries matched: 4
gi|27573538
Mass: 15944
Score: 121
Queries matched: 4
gi|27573539
Mass: 15836
Score: 121
Queries matched: 4
gi|27573540
Mass: 15811
Score: 121
Queries matched: 4
36.
gi|157164773
Mass: 24977
Score: 117
Queries matched: 3
emPAI: 0.46
Query
596
598
672
37.
Observed
810.4066
818.4038
900.4548
Mr(expt)
1618.7986
1634.7930
1798.8950
Mr(calc)
1618.7985
1634.7934
1798.8949
0.11
0
56 0.0034
1
-0.21
0
(38)
0.24
1
0.10
0
61 0.0009
1
gi|194038353
Mass: 47192
Score: 111
Queries matched: 2
Peptide
K.TGTVTMDVAQAVNNAK.S
K.TGTVTMDVAQAVNNAK.S + Oxidation (M)
K.AAGADIVGADDLVEDIQK.G
emPAI: 0.07
Query
160
277
38.
Observed
493.7795
561.3135
Mr(expt)
985.5444
1120.6124
Mr(calc)
985.5444
1120.6128
0.05
0
43
0.058
1
-0.33
0
68 0.00016
1
gi|154175458
Mass: 18862
Score: 105
ProP protein [Campylobacter curvus 525.92]
Peptide
K.QLVSLDPSK.N
R.DTQSIIFLGK.V
Queries matched: 4
Query
175
198
337
541
Observed
500.2482
510.2688
613.7892
755.3721
Mr(expt)
998.4818
1018.5230
1225.5638
1508.7296
Mr(calc)
998.4821
1018.5236
1225.5649
1508.7300
-0.28
0
22
6.8
1
-0.58
0
26
5.5
1
-0.84
0
26
1.9
1
-0.21
0
32
0.73
1
Mass: 18808
Score: 105
gi|157165691
Peptide
K.FHIDNPEK.Q
K.FQWTGGPVK.K.MDTGVGNYELK.F
K.NGFGEGEWIPYLK.I
Queries matched: 4
12/9/2009
Page 11 of 22
39.
gi|157164497
Mass: 29815
Score: 100
Queries matched: 3
fumarate hydratase [Campylobacter concisus 13826]
emPAI: 0.11
Query
247
292
517
40.
Observed
543.7824
573.8110
736.3907
Mr(expt)
1085.5502
1145.6074
1470.7668
Mr(calc)
1085.5505
1145.6081
1470.7679
-0.27
1
28
1.7
1
-0.54
0
31
1.4
1
-0.68
0
40
0.11
1
gi|157164023
Mass: 34756
Score: 97
Queries matched: 2
carbon-nitrogen family hydrolase [Campylobacter concisus 13826]
Peptide
K.GYTEGYLRK.S
K.SVVAEPLFER.K
K.TGVGPQGLGGDTTAIK.V
emPAI: 0.20
Query
431
644
41.
Observed
661.8633
863.9539
Mr(expt)
1321.7120
1725.8932
Mr(calc)
1321.7129
1725.8938
-0.65
0
47
0.011
1
-0.30
0
50 0.0096
1
gi|157165597
Mass: 20109
Score: 93
Queries matched: 3
CRISPR-associated protein Cas2 [Campylobacter concisus 13826]
Peptide
K.ILILSEGDYATK.V
K.INTGFLVYGVGLSSDGK.I
emPAI: 0.17
Query
233
659
660
42.
Observed
532.7902
588.2806
881.9175
Mr(expt)
1063.5658
1761.8200
1761.8204
Mr(calc)
1063.5662
1761.8210
1761.8210
gi|3928489
Mass: 222566
Score: 90
-0.31
0
61 0.0013
1
-0.57
1
32
0.57
1
-0.30
1
(29)
1.3
1
Peptide
K.YNLDIISAR.C
K.SSDKQFYVFNDVDAK.M
K.SSDKQFYVFNDVDAK.M
Queries matched: 6
Query
59
67
69
74
189
190
Observed
417.7285
421.7708
421.7711
425.7260
505.8157
505.8157
Mr(expt)
833.4424
841.5270
841.5276
849.4374
1009.6168
1009.6168
Mr(calc)
833.4429
841.5273
841.5273
849.4378
1009.6172
1009.6172
-0.56
0
30
2.4
1
-0.30
0
32
0.38
1
0.42
0
(21)
4.1
1
-0.46
0
(28)
3.5
4
-0.35
0
28
0.32
1
-0.35
0
(27)
0.38
1
Peptide
R.LQSMTVR.Q
K.LTIPAVTK.A
K.LTIPAVTK.A
R.LQSMTVR.Q + Oxidation (M)
R.VTGIPTPVVK.F
R.VTGIPTPVVK.F
gi|17066104
Mass: 2991182 Score: 90
Queries matched: 6
Mass: 3813840 Score: 90
Queries matched: 6
gi|17066105
gi|17066106
Mass: 631230
Score: 90
Queries matched: 6
gi|33871368
Mass: 32739
Score: 90
Queries matched: 6
Mass: 60186
Score: 90
Queries matched: 6
gi|37573987
gi|47124504
Mass: 69051
Score: 90
Queries matched: 6
Mass: 21554
Score: 90
Queries matched: 6
gi|85543964
gi|108861911
Mass: 3813810 Score: 90
Queries matched: 6
gi|110349713
Mass: 3004958 Score: 90
Queries matched: 6
gi|110349715
Mass: 2991151 Score: 90
Queries matched: 6
gi|110349717
Mass: 3012156 Score: 90
Queries matched: 6
gi|110349719
Mass: 3711359 Score: 90
Queries matched: 6
gi|110349721
Mass: 631174
Score: 90
Queries matched: 6
gi|110590764
Mass: 20706
Score: 90
Queries matched: 6
gi|119631418
Mass: 3878844 Score: 90
Queries matched: 6
gi|119631420
Mass: 2991188 Score: 90
Queries matched: 6
gi|119631421
Mass: 631293
Score: 90
Queries matched: 6
gi|119631423
Mass: 3004995 Score: 90
Queries matched: 6
12/9/2009
Page 12 of 22
gi|119631424
Mass: 3012223 Score: 90
Queries matched: 6
gi|194043971
Mass: 147632
Score: 90
Queries matched: 6
gi|225131084
Mass: 3711397 Score: 90
Queries matched: 6
gi|194222358
Mass: 3708275 Score: 88
Queries matched: 6
43.
gi|157165213
Mass: 19144
Score: 89
Queries matched: 2
emPAI: 0.18
Query
135
543
44.
Observed
475.7505
755.8906
Mr(expt)
949.4864
1509.7666
Mr(calc)
949.4869
1509.7675
-0.44
0
38
0.29
1
-0.56
1
51
0.01
1
Peptide
K.ADAIIEYR.K
K.TFDNLKGEISTTGK.S
gi|416751
Mass: 26372
Score: 88
Queries matched: 2
emPAI: 0.27
RecName: Full=Alpha-S2-casein; Short=Alpha-S2-CN; Flags: Precursor
Query
360
462
Observed
623.8254
684.3515
Mr(expt)
1245.6362
1366.6884
Mr(calc)
1245.6353
1366.6881
0.72
1
42
0.1
1
0.27
0
45
0.038
1
Peptide
K.ITVDDKHYQK.A
K.ALNEINQFYQK.F
Mass: 26357
Score: 88
Queries matched: 2
gi|707034
alpha s2-casein C [Capra hircus]
gi|4127680
Mass: 5795
Score: 88
Queries matched: 2
alpha s2-casein [Capra hircus]
Mass: 7492
Score: 88
Queries matched: 2
gi|4375863
gi|4584554
Mass: 24684
Score: 88
Queries matched: 2
aS2-casein [Bubalus bubalis]
gi|6179400
Mass: 26416
Score: 88
Queries matched: 2
Mass: 14524
Score: 88
Queries matched: 2
gi|8439285
gi|8439287
Mass: 26386
Score: 88
Queries matched: 2
gi|15706248
Mass: 26415
Score: 88
Queries matched: 2
gi|27806963
Mass: 26002
Score: 88
Queries matched: 2
casein alpha-S2 [Bos taurus]
gi|57164371
Mass: 26315
Score: 88
Queries matched: 2
alpha-S2-casein [Ovis aries]
Mass: 26099
Score: 88
Queries matched: 2
gi|71982842
alpha s2-casein [Bubalus bubalis]
gi|73671241
Mass: 26112
Score: 88
Queries matched: 2
alpha s2 casein [Bubalus bubalis]
gi|211919052
Mass: 26098
Score: 88
Queries matched: 2
alpha s2 casein precursor [Bubalus bubalis]
Mass: 25048
Score: 88
Queries matched: 2
gi|211919054
alpha s2 casein precursor [Bubalus bubalis]
45.
gi|57164373
Mass: 69143
Score: 85
Queries matched: 4
Query
31
32
71
193
Observed
395.2391
395.2393
424.2554
507.8129
Mr(expt)
788.4636
788.4640
846.4962
1013.6112
Mr(calc)
788.4644
788.4644
846.4963
1013.6121
-0.92
0
24
2.4
1
-0.41
0
(22)
4.5
2
-0.09
1
33
0.27
1
-0.82
0
28
0.9
1
gi|193085052
Mass: 66270
Score: 85
46.
Peptide
K.IVTDLTK.V
K.IVTDLTK.V
R.LSQKFPK.A
K.QTALVELLK.H
Queries matched: 4
gi|119914036
Mass: 34009
Score: 83
Queries matched: 2
PREDICTED: similar to Endopin 1b isoform 1 [Bos taurus]
emPAI: 0.10
Query
58
737
47.
Observed
417.7048
1021.9890
gi|3914346
Mr(expt)
833.3950
2041.9634
Mass: 82383
Mr(calc)
833.3953
2041.9633
Score: 83
-0.28
0
39
0.13
1
0.09
0
44
0.047
1
Peptide
K.MQDLEAK.L
R.VLYSSEAFPTNFGDPEAAK.S
Queries matched: 3
12/9/2009
Page 13 of 22
Query
140
154
321
48.
Observed
478.7742
488.2789
596.8198
Mr(expt)
955.5338
974.5432
1191.6250
Mr(calc)
955.5338
974.5437
1191.6248
0.00
0
24
3.1
1
-0.43
0
31
1.6
1
0.25
1
29
1.9
1
gi|47523844
Mass: 47164
Score: 83
Queries matched: 2
serine proteinase inhibitor, clade A, member 1 [Sus scrofa]
Peptide
K.VVQGEPSLK.V
R.WISTVELK.V
R.GLIKEQYEGR.L
emPAI: 0.07
Query
195
718
49.
Observed
508.3106
982.4791
Mr(expt)
1014.6066
1962.9436
gi|291464
Mass: 22987
Mr(calc)
1014.6073
1962.9422
Score: 80
-0.67
0
32
0.46
1
0.73
0
51
0.01
1
Peptide
K.SLLGNLGITK.V
K.VFSDEADLSGVTEEQPLK.L
Queries matched: 4
Query
331
532
540
626
Observed
604.3068
746.3466
753.3543
844.4525
Mr(expt)
1206.5990
1490.6786
1504.6940
1686.8904
Mr(calc)
1206.5993
1490.6784
1504.6940
1686.8901
-0.21
1
21
9.4
1
0.18
0
(30)
0.63
1
0.02
0
36
0.19
1
0.22
0
22
6
1
gi|291466
Mass: 22671
Score: 80
Queries
gi|291472
Mass: 21971
Score: 80
Queries
Mass: 17457
Score: 80
Queries
gi|291476
gi|291480
Mass: 23493
Score: 80
Queries
50.
gi|291462
Mass: 20404
Score: 78
Peptide
R.ASGVPDRFSGSK.S
K.SSSSVSCQVTHQGK.T + Carbamidomethyl (C)
K.SSSSVSCQVTHQGK.T + Acrylamide (C)
K.VNGAATTQGVQTTKPSK.Q
matched: 4
matched: 4
matched: 4
matched: 4
Queries matched: 4
Query
331
532
540
637
51.
Observed
604.3068
746.3466
753.3543
850.4379
Mr(expt)
1206.5990
1490.6786
1504.6940
1698.8612
Mr(calc)
1206.5993
1490.6784
1504.6940
1698.8617
-0.21
1
21
9.4
1
0.18
0
(30)
0.63
1
0.02
0
36
0.19
1
-0.26
0
20
10
1
Peptide
R.ASGVPDRFSGSK.S
K.YAASSYLTLTPAQWK.S
gi|157165672
Mass: 23664
Score: 77
Queries matched: 2
emPAI: 0.14
curli production assembly/transport component CsgG subfamily protein [Campylobacter concisus 13826]
Query
22
176
52.
Observed
375.7107
501.2849
Mr(expt)
749.4068
1000.5552
Mr(calc)
749.4072
1000.5553
-0.44
0
21
17
1
-0.06
0
57 0.0037
1
Peptide
R.FSVLER.S
K.VNLNVVDTK.T
gi|157165278
Mass: 26679
Score: 75
Queries matched: 3
53.
Query
272
304
Observed
559.3054
580.8112
Mr(expt)
1116.5962
1159.6078
Mr(calc)
1116.5961
1159.6084
491
709.3621
1416.7096
1416.7096
0.13
1
26
4.5
2
-0.50
1
23
4.5
1
0.03
1
25
3.5
1
Peptide
K.DKLVAAMNQK.I
K.KTELEGNLEK.I
R.LDEEVNDLKSQK.S
gi|438069
Mass: 21843
Score: 72
Queries matched: 2
thiol-specific antioxidant protein [Homo sapiens]
Query
88
333
Observed
431.7550
606.3408
Mr(expt)
861.4954
1210.6670
Mr(calc)
861.4960
1210.6670
-0.64
0
28
1.1
1
0.05
0
44
0.028
1
Peptide
R.GLFIIDGK.G
R.QITVNDLPVGR.S
gi|440308
Mass: 21877
Score: 72
Queries matched: 2
enhancer protein
gi|1617118
Mass: 18315
Score: 72
Queries matched: 2
12/9/2009
Page 14 of 22
TSA [Homo sapiens]
gi|1717797
Mass: 14158
Score: 72
Queries matched: 2
RecName: Full=Peroxiredoxin-2; AltName: Full=Thioredoxin peroxidase 1; AltName: Full=Thioredoxin-dependent peroxide reductase 1; AltName: Full=Thiolgi|9955007
Mass: 21795
Score: 72
Queries matched: 2
Chain A, Thioredoxin Peroxidase B From Red Blood Cells
gi|32189392
Mass: 21878
Score: 72
Queries matched: 2
peroxiredoxin 2 isoform a [Homo sapiens]
gi|60654143
Mass: 21991
Score: 72
Queries matched: 2
peroxiredoxin 2 [synthetic construct]
gi|60830247
Mass: 21979
Score: 72
Queries matched: 2
gi|60835754
Mass: 21993
Score: 72
Queries matched: 2
gi|62901916
Mass: 24797
Score: 72
Queries matched: 2
thioredoxin peroxidase 1-like [synthetic construct]
gi|73986497
Mass: 21884
Score: 72
Queries matched: 2
PREDICTED: similar to Peroxiredoxin 2 (Thioredoxin peroxidase 1) (Thioredoxin-dependent peroxide reductase 1) (Thiol-specific antioxidant protein) (T
gi|77176934
Mass: 10926
Score: 72
Queries matched: 2
peroxiredoxin 2 [Spermophilus tridecemlineatus]
gi|109123565
Mass: 16107
Score: 72
Queries matched: 2
PREDICTED: peroxiredoxin 2 isoform 2 [Macaca mulatta]
gi|109123567
Mass: 16108
Score: 72
Queries matched: 2
Mass: 19458
Score: 72
Queries matched: 2
gi|109123569
gi|194213066
Mass: 21917
Score: 72
Queries matched: 2
PREDICTED: similar to Peroxiredoxin 2 [Equus caballus]
gi|194375974
Mass: 20095
Score: 72
Queries matched: 2
gi|196008113
Mass: 21705
Score: 72
Queries matched: 2
gi|197100696
Mass: 19418
Score: 72
Queries matched: 2
peroxiredoxin 2 [Pongo abelii]
54.
gi|158604975
Mass: 28432
Score: 67
Queries matched: 2
emPAI: 0.12
D-methionine-binding lipoprotein MetQ [Campylobacter concisus 13826]
Query
266
617
55.
Observed
556.3033
835.9025
Mr(expt)
1110.5920
1669.7904
gi|165945
Mass: 53512
Mr(calc)
1110.5921
1669.7907
Score: 65
-0.04
0
28
0.97
1
-0.18
0
39
0.12
1
Peptide
K.TPLDIVDNPK.K
K.DGAVVSIPNDPTNESR.A
Queries matched: 4
Query
252
263
379
380
Observed
547.2811
554.2892
633.8089
633.8092
Mr(expt)
1092.5476
1106.5638
1265.6032
1265.6038
Mr(calc)
1092.5485
1106.5641
1265.6040
1265.6040
-0.79
0
(23)
6.6
1
-0.27
0
28
2.2
1
-0.62
0
(32)
0.84
1
-0.15
0
37
0.26
1
Peptide
K.ALETHTYFER.H
K.ALETHTYFER.H
Mass: 64480
Score: 65
Queries matched: 4
gi|478694
Ig mu chain - sheep
Mass: 52124
Score: 65
Queries matched: 4
gi|561876
This CDS feature is included to show the translation of the corresponding C_region. Presently translation qualifiers on C_region features are illegal
gi|2232299
Mass: 47885
Score: 65
Queries matched: 4
gi|3834667
Mass: 48183
Score: 65
Queries matched: 4
gi|24496448
Mass: 65621
Score: 65
Queries matched: 4
Mass: 49106
Score: 65
Queries matched: 4
gi|33413902
Mass: 49909
Score: 65
Queries matched: 4
gi|34538498
gi|162424563
Mass: 52197
Score: 65
Queries matched: 4
immunoglobulin mu heavy chain constant region [Capra hircus]
56.
gi|27807521
Mass: 21216
Score: 61
Queries matched: 3
prostaglandin D2 synthase, brain [Bos taurus]
emPAI: 0.16
Query
62
485
486
Observed
421.2152
469.9135
704.3672
Mr(expt)
840.4158
1406.7187
1406.7198
Mr(calc)
840.4164
1406.7194
1406.7194
-0.60
0
28
1.6
1
-0.52
1
(21)
16
1
0.31
1
33
0.84
1
Peptide
R.MATLYSR.S
R.AEVKEHFTTFAK.S
R.AEVKEHFTTFAK.S
12/9/2009
57.
gi|157163886
Mass: 26131
Score: 61
Queries matched: 2
thiol peroxidase [Campylobacter concisus 13826]
Page 15 of 22
emPAI: 0.13
Query
257
258
58.
Observed
550.3214
550.3216
Mr(expt)
1098.6282
1098.6286
Mr(calc)
1098.6285
1098.6285
-0.21
0
(52) 0.0042
1
0.15
0
61 0.00059
1
gi|157165326
Mass: 18857
Score: 60
Queries matched: 3
Peptide
K.VAGDIEAVVVK.L
K.VAGDIEAVVVK.L
emPAI: 0.18
Query
312
465
466
59.
Observed
584.7725
685.8586
685.8592
Mr(expt)
1167.5304
1369.7026
1369.7038
Mr(calc)
1167.5309
1369.7030
1369.7030
-0.37
0
26
2.6
1
-0.28
0
(26)
2.9
1
0.60
0
35
0.42
1
gi|157165161
Mass: 24936
Score: 59
Queries matched: 2
triosephosphate isomerase [Campylobacter concisus 13826]
Peptide
K.AGGHWDPAETK.M
K.TNYGVAFFPNLK.G
K.TNYGVAFFPNLK.G
emPAI: 0.13
Query
320
468
60.
Observed
596.8141
689.8458
Mr(expt)
1191.6136
1377.6770
Mr(calc)
1191.6135
1377.6776
0.10
0
24
6.4
1
-0.38
0
35
0.43
1
Peptide
R.QLENIDLGYK.N
R.EILNESEEFLR.S
gi|157165400
Mass: 23675
Score: 58
Queries matched: 2
nitroreductase family protein [Campylobacter concisus 13826]
Query
206
275
Observed
512.2805
560.8292
Mr(expt)
1022.5464
1119.6438
Mr(calc)
1022.5470
1119.6441
-0.55
0
24
1.7
1
-0.20
0
33
0.32
1
Peptide
-.MNYLEILK.F
R.VAIVVPFGYR.L
gi|255323030
Mass: 24186
Score: 58
Queries matched: 2
putative NAD(P)H nitroreductase YfkO [Campylobacter showae RM3277]
61.
gi|28317
Mass: 59492
Score: 57
Queries matched: 2
Query
348
469
Observed
617.8431
691.3274
Mr(expt)
1233.6716
1380.6402
Mr(calc)
1233.6717
1380.6408
-0.04
1
29
1.3
1
-0.43
0
28
2.3
1
Peptide
R.LKYENEVALR.Q
R.ALEESNYELEGK.I
gi|307086
Mass: 46359
Score: 57
Queries matched: 2
keratin-10 [Homo sapiens]
Mass: 57213
Score: 57
Queries matched: 2
gi|623409
gi|21961605
Mass: 58792
Score: 57
Queries matched: 2
gi|61740600
Mass: 57676
Score: 57
Queries matched: 2
gi|109115260
Mass: 62063
Score: 57
Queries matched: 2
gi|109115262
Mass: 57547
Score: 57
Queries matched: 2
gi|109115264
Mass: 56705
Score: 57
Queries matched: 2
gi|114667507
Mass: 100282
Score: 57
Queries matched: 2
gi|114667509
Mass: 62761
Score: 57
Queries matched: 2
gi|114667511
Mass: 58244
Score: 57
Queries matched: 2
gi|114667513
Mass: 56692
Score: 57
Queries matched: 2
gi|119581085
Mass: 63308
Score: 57
Queries matched: 2
gi|195972866
Mass: 58766
Score: 57
Queries matched: 2
gi|251757513
Mass: 58818
Score: 57
Queries matched: 2
62.
gi|157164686
Mass: 31540
Score: 54
Queries matched: 1
emPAI: 0.11
12/9/2009
Page 16 of 22
Query
687
63.
Observed
917.4447
Mr(expt)
1832.8748
Mr(calc)
1832.8752
-0.17
0
54
0.004
1
Peptide
R.SVANANSVENLDSSDIAK.S
gi|149716548
Mass: 30312
Score: 52
Queries matched: 1
emPAI: 0.11
PREDICTED: similar to Apolipoprotein A-I precursor (Apo-AI) (ApoA-I) [Equus caballus]
Query
489
Observed
706.8564
Mr(expt)
1411.6982
Mr(calc)
1411.6983
-0.05
0
52 0.0069
1
Peptide
R.EYVAQFEASALGK.Q
Mass: 30485
Score: 52
Queries matched: 1
gi|184185461
apolipoprotein A-I (predicted) [Rhinolophus ferrumequinum]
64.
gi|154173631
Mass: 19261
Score: 50
Queries matched: 1
emPAI: 0.17
periplasmic nitrate reductase, diheme cytochrome c subunit [Campylobacter curvus 525.92]
Query
405
Observed
643.8514
Mr(expt)
1285.6882
Mr(calc)
1285.6878
0.37
0
50
0.011
1
Peptide
K.SNLLDVLNDGVK.-
gi|157165227
Mass: 19284
Score: 50
Queries matched: 1
periplasmic nitrate reductase, diheme cytochrome c subunit [Campylobacter concisus 13826]
65.
gi|149925600
Mass: 70853
Score: 49
Queries matched: 1
probable two-component sensor histidine kinase [Limnobacter sp. MED105]
Query
143
Observed
480.7797
Mr(expt)
959.5448
Mr(calc)
959.5440
0.89
0
49
0.014
1
Peptide
R.VEALYIPR.F
Query
158
300
652
153
432
194
272
81
40
94
684
319
711
214
686
132
547
74
494
683
444
488
155
197
518
551
679
113
299
45
112
48
97
316
616
769
5
199
Observed
492.3033
579.2986
875.9168
487.7796
663.3204
508.2803
559.3054
428.7661
403.2445
435.7739
914.4418
596.3181
650.9786
516.7877
610.3012
474.7626
505.9110
425.7260
710.8639
914.4412
676.3587
704.8225
488.2789
508.7719
736.4036
763.8961
907.9409
454.7453
577.8356
404.7316
453.7500
407.7480
439.2439
589.3069
834.9668
748.0487
353.7158
510.7718
Mr(expt)
982.5920
1156.5826
1749.8190
973.5446
1324.6262
1014.5460
1116.5962
855.5176
804.4744
869.5332
1826.8690
1190.6216
1949.9140
1031.5608
1827.8818
947.5106
1514.7112
849.4374
1419.7132
1826.8678
1350.7028
1407.6304
974.5432
1015.5292
1470.7926
1525.7776
1813.8672
907.4760
1153.6566
807.4486
905.4854
813.4814
876.4732
1176.5992
1667.9190
2241.1243
705.4170
1019.5290
Mr(calc)
982.5923
1156.5836
1749.8210
973.5444
1324.6259
1014.5458
1116.5927
855.5178
804.4745
869.5334
1826.8686
1190.6217
1949.9153
1031.5611
1827.8825
947.5076
1514.7113
849.4378
1419.7146
1826.8686
1350.7006
1407.6306
974.5396
1015.5298
1470.7930
1525.7776
1813.8669
907.4763
1153.6567
807.4491
905.4827
813.4782
876.4705
1176.5986
1667.9206
2241.1237
705.4174
1019.5321
ppm Miss Score
-0.29
0
48
-0.87
0
43
-1.09
0
43
0.27
1
42
0.27
0
42
0.27
1
42
3.14
1
42
-0.19
0
41
-0.11
0
41
-0.23
0
41
0.22
0
39
-0.01
0
39
-0.66
1
38
-0.27
0
38
-0.41
0
38
3.22
1
37
-0.12
0
37
-0.44
0
37
-0.98
0
37
-0.43
0
37
1.69
1
37
-0.14
0
36
3.70
0
36
-0.56
0
36
-0.24
0
35
0.01
0
35
0.20
0
35
-0.28
0
34
-0.08
1
34
-0.52
0
34
3.07
0
32
3.96
0
32
3.13
0
32
0.55
0
31
-0.94
0
30
0.27
0
30
-0.44
0
30
-3.01
0
30
Expect Rank
0.0063
1
0.029
1
0.048
1
0.079
1
0.057
1
0.11
1
0.12
1
0.092
1
0.041
1
0.1
1
0.14
1
0.13
1
0.21
1
0.15
1
0.19
1
0.33
1
0.15
1
0.48
1
0.27
1
0.24
1
0.27
1
0.15
1
0.48
1
0.42
1
0.33
1
0.27
1
0.37
1
0.69
1
0.28
1
0.61
1
0.63
1
1.3
1
1.6
1
0.9
1
0.47
1
0.97
1
0.99
1
1.8
1
Peptide
ALGILNLNR
QGVDADINGLR
TGASNYYLGEVAYSQK
KADIETIGK
STDAYELSVANR
DNIDAAIKR
KDLPHAPDPK
LATVVTPR
VFLNISI
VVSLSIPR
GEADALNLDGGYIYTAGK
AAEGCGEYNKDAIQSLPK + Carbamidomethyl (C)
VGSGSASLGGIK
LGENCDADILFVHSPK + Acrylamide (C)
FAELLRAE
VGAHAGDYGAEALER
LQSIMSR + Oxidation (M)
YVNWIQQTIAAN
GEADALNLDGGYIYTAGK
KMGWPLESYLK
AADETWEPFASGK
STAGASILGAK
DALSIQDVR
ISPDTQGVLVTDVK
LYDQLLQSYQQK
LGENCDADILFVHSPK + Carbamidomethyl (C)
QSEYLIR
SRLPGIVAEGR
LSVGGTFK
MSVVMIAR
CPVALIK + Acrylamide (C)
FINAGLDK
SLEQSSATLNK
ILILDGTQGNAGVAQAK
AISSAIQAANIGVNPNSDGESVK
YVVGIR
MNSVTELVK
12/9/2009
Page 17 of 22
207
243
87
21
11
438
119
285
117
282
343
513.7950
539.7981
431.2466
373.2443
359.2163
674.3354
465.7194
568.3027
460.2219
564.3137
615.3472
1025.5754
1077.5816
860.4786
744.4740
716.4180
1346.6562
929.4242
1134.5908
918.4292
1126.6128
1228.6798
1025.5757
1077.5818
860.4789
744.4745
716.4181
1346.6612
929.4243
1134.5921
918.4294
1126.6135
1228.6816
-0.26
-0.19
-0.34
-0.65
-0.06
-3.70
-0.04
-1.09
-0.18
-0.57
-1.42
0
0
1
0
0
1
0
0
0
0
0
29
29
29
29
28
28
28
28
28
28
27
0.46
1.8
2.9
0.4
3.9
1.7
0.7
1.7
1.2
1.7
2.3
1
1
1
1
1
1
1
1
1
1
1
VLEGVGIDPK
LSGIQNFTAK
MNIEKVK
TITGILK
VSVASVR
MADKVALADNAGR + Oxidation (M)
YFTENTR
FVSEALGADVK
TDLENEAK
FAHVVAASTPK
IQGVPAFVVDGK
269
182
46
83
57
127
592
49
236
56
570
338
231
439
557.7981
503.7636
404.7317
429.2110
416.2501
470.2968
807.4367
409.7239
534.2762
416.1956
794.4047
613.8240
532.2672
674.3356
1113.5816
1005.5126
807.4488
856.4074
830.4856
938.5790
1612.8588
817.4332
1066.5378
830.3766
1586.7948
1225.6334
1062.5198
1346.6566
1113.5778
1005.5165
807.4491
856.4079
830.4861
938.5800
1612.8582
817.4334
1066.5407
830.3770
1586.7940
1225.6343
1062.5168
1346.6612
3.41
-3.80
-0.25
-0.53
-0.60
-1.07
0.42
-0.18
-2.68
-0.44
0.52
-0.66
2.85
-3.40
1
0
1
0
1
0
0
0
0
0
0
0
0
1
27
27
27
27
26
26
26
25
25
25
25
25
25
24
2.2
4
2.9
0.97
4.5
0.5
2.9
1.7
3.8
1.2
3.6
3.5
4.6
4.4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
GGKVTGEVDPR
AMNLEVVSK + Oxidation (M)
TVADKFK
QAYSNFK
KASGDLLK
IAVEVAPLK
LHYVGLCLLCIPR + 2 Carbamidomethyl (C)
VDASWLK
IFSSGINSSR
DEVTDPR
TPSEWQETGIIAGAK
DGYLIANIYGK
VPPTEAFMR + Oxidation (M)
26
647
562
55
32
487
262
318
597
42
336
167
72
169
191
36
563
208
223
152
311
133
196
89
296
34
313
340
381.7285
865.4134
783.8727
415.7294
395.2393
704.8223
553.3054
590.8522
545.6302
403.7112
610.8481
496.7921
424.7308
496.7980
506.2624
398.2034
783.8727
514.7850
527.3185
487.2874
584.7687
475.7144
508.7480
432.7448
575.3110
397.7057
585.8577
614.8427
761.4424
1728.8122
1565.7308
829.4442
788.4640
1407.6300
1104.5962
1179.6898
1633.8688
805.4078
1219.6816
991.5696
847.4470
991.5814
1010.5102
794.3922
1565.7308
1027.5554
1052.6224
972.5602
1167.5228
949.4142
1015.4814
863.4750
1148.6074
793.3968
1169.7008
1227.6708
761.4436
1728.8141
1565.7251
829.4446
788.4644
1407.6306
1104.5927
1179.6903
1633.8749
805.4082
1219.6812
991.5702
847.4473
991.5815
1010.5073
794.3923
1565.7283
1027.5563
1052.6230
972.5604
1167.5230
949.4109
1015.4822
863.4753
1148.6077
793.3970
1169.7019
1227.6711
-1.48
-1.07
3.64
-0.47
-0.41
-0.42
3.19
-0.42
-3.77
-0.50
0.34
-0.56
-0.31
-0.00
2.94
-0.03
1.63
-0.83
-0.48
-0.12
-0.13
3.47
-0.72
-0.25
-0.22
-0.21
-0.94
-0.18
0
0
0
0
0
0
1
0
0
1
1
1
0
1
0
0
1
0
0
1
0
0
0
0
0
0
0
0
24
24
24
24
24
24
24
24
24
24
24
23
23
23
23
23
23
22
22
22
22
22
22
22
22
21
21
21
4.2
3.8
4.1
3.4
2.5
2.3
6.7
0.94
3.6
3.8
3.3
4.7
8.6
1.9
3.9
8.1
6
4.3
1.3
7.9
4.8
5
4
11
8.2
13
2.5
7.2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
IFLQGGK
LSFNPTQLEEQCHI + Acrylamide (C)
MLGEEICLCIAGNK + Carbamidomethyl (C); Oxidation (M)
GLGVYHGK
IVTVETK
AADETWEPFASGK
FLDRLAENK
VLIVVSPYYK
YITGATLPVDAGCLIK
FARQGEV
VGAKDVYLDIK
KYLISIGAQ
IMAEASVK
KTLPAPTHK
IFYNTPEK
GVYVSDR
HAVALFSAGR
LLAAAGALPEK
NLIKAGETK
MDAAAEFLER + Oxidation (M)
TPELEENGK
ALVVGDYK
INLDYAAEIK
FVGENTK
ALDVLASAGLIK
DLDPQISVITK
720
530
156
440
235
371
80
356
284
41
577
347
676
61
655.6514
745.4223
490.2841
674.3356
533.2640
630.2974
428.7661
622.3451
565.7697
403.7112
799.4471
615.8566
900.4908
417.7288
1963.9324
1488.8300
978.5536
1346.6566
1064.5134
1258.5802
855.5176
1242.6756
1129.5248
805.4078
1596.8796
1229.6986
1798.9670
833.4430
1963.9309
1488.8334
978.5538
1346.6612
1064.5172
1258.5763
855.5178
1242.6720
1129.5251
805.4082
1596.8797
1229.6979
1798.9651
833.4429
0.75
-2.25
-0.20
-3.40
-3.54
3.15
-0.15
2.90
-0.25
-0.50
-0.02
0.59
1.06
0.19
1
1
0
1
0
1
0
1
0
1
0
1
0
1
21
21
21
21
20
20
20
20
20
20
20
20
20
20
7.8
4.3
9.3
11
13
6.6
12
9.2
6
8.7
6.4
7.3
8.4
19
1
1
1
1
1
1
1
1
1
1
1
1
1
1
ASCLKLVSGVTGLGGK
FAISVPFAK
SQCLLTTDK + Carbamidomethyl (C)
GNGGNGGDGTRAAR
IAISLPSR
IQKSFENHLK
DQPTIEQGDK
FARQGEV
VVPSEDTVPLIMGLK
KLEIVQTSNAK
IKSMNNK
98
271
344
3
110
502
96
412
101
439.7526
558.8297
615.8271
351.7233
453.7209
718.3751
437.7531
651.8396
442.2496
877.4906
1115.6448
1229.6396
701.4320
905.4272
1434.7356
873.4916
1301.6646
882.4846
877.4909
1115.6451
1229.6438
701.4323
905.4276
1434.7354
873.4920
1301.6687
882.4858
-0.29
-0.22
-3.39
-0.40
-0.44
0.15
-0.37
-3.15
-1.28
0
1
0
0
0
0
1
1
1
20
19
19
19
19
19
19
19
19
12
8.7
18
13
20
15
30
16
4.6
1
1
1
1
1
1
1
1
1
LITEQFK
RIDALYLPR
QGCIDLALGGVK + Carbamidomethyl (C)
VTEIIK
NIDEMIR + Oxidation (M)
EQLNENISTFIK
EAVRITVS
SQNGRPATSKEK
LLGCGRHK
12/9/2009
Page 18 of 22
238
584
240
85
719
212
615
137
136
16
305
537.2953
800.9380
538.2663
429.7504
982.9716
515.7920
834.9261
478.2531
476.2606
363.6945
581.7905
1072.5760
1599.8614
1074.5180
857.4862
1963.9286
1029.5694
1667.8376
954.4916
950.5066
725.3744
1161.5664
1072.5764
1599.8620
1074.5193
857.4858
1963.9309
1029.5715
1667.8366
954.4883
950.5073
725.3748
1161.5699
-0.34
-0.37
-1.17
0.49
-1.14
-1.98
0.62
3.53
-0.66
-0.53
-3.01
0
0
0
0
1
0
0
0
0
0
0
19
19
19
19
19
19
19
19
19
19
19
15
13
10
21
20
14
15
10
16
2.2
20
1
1
1
1
1
1
1
1
1
1
1
IADELLTNGK
LLDKPLFTPDANTR
SDEVGEQLAK
IVEPVSSK
LIVMCVGPK + Acrylamide (C)
IIPSAEDPSQDIVER
ILSGESHGR
SLINDYVK
FIDGFK
MINPGSEISSK
358
539
599
120
90
453
408
222
367
7
51
399
560
503
623.2891
752.8679
818.8864
465.7663
432.7490
453.2695
431.5720
526.2640
626.3584
356.1782
411.7395
426.8853
778.3589
721.3405
1244.5636
1503.7212
1635.7582
929.5180
863.4834
1356.7867
1291.6942
1050.5134
1250.7022
710.3418
821.4644
1277.6341
1554.7032
1440.6664
1244.5648
1503.7239
1635.7596
929.5182
863.4865
1356.7878
1291.6958
1050.5128
1250.7023
710.3421
821.4647
1277.6326
1554.7024
1440.6667
-0.94
-1.76
-0.84
-0.15
-3.51
-0.79
-1.30
0.63
-0.02
-0.40
-0.30
1.16
0.52
-0.18
0
0
1
0
0
0
1
0
0
0
0
0
0
1
19
18
18
18
18
18
18
18
18
18
18
18
18
18
8.1
15
13
26
25
5.1
11
23
9.2
19
19
19
11
14
1
1
1
1
1
1
1
1
1
1
1
1
1
1
ACPGPYYVYR + Carbamidomethyl (C)
IQAELAQMAGDDVK + Oxidation (M)
EIDAMDGLMGRVADK + Oxidation (M)
ITVVQNEK
VATLAAYR
GHALIINLPGQPK
VRFPIVADMTK + Oxidation (M)
IYILFNLNNK
FMEIR + Oxidation (M)
VTAYAVAK
QGDIMIFTIGPA + Oxidation (M)
FCNDLQLSPDEFK
HCPKTDIENAEK + Carbamidomethyl (C)
50
447
8
265
188
79
726
226
373
587
529
397
437
170
709
422
122
315
413
569
287
778
675
674
673
496
611
699
410.7225
676.8860
356.2131
555.3316
505.7062
428.7192
661.6873
529.2804
630.3423
534.2950
496.2666
426.5500
673.3253
497.7819
647.6498
656.3197
467.2447
588.3173
651.8397
790.9166
572.3218
804.6763
600.6622
900.4896
600.6617
712.8535
555.2863
961.5587
819.4304
1351.7574
710.4116
1108.6486
1009.3978
855.4238
1982.0401
1056.5462
1258.6700
1599.8632
1485.7780
1276.6282
1344.6360
993.5492
1939.9276
1310.6248
932.4748
1174.6200
1301.6648
1579.8186
1142.6290
2411.0071
1798.9648
1798.9646
1798.9633
1423.6924
1662.8371
1921.1028
819.4313
1351.7572
710.4115
1108.6492
1009.3957
855.4239
1982.0394
1056.5452
1258.6703
1599.8620
1485.7722
1276.6299
1344.6350
993.5495
1939.9244
1310.6289
932.4716
1174.6193
1301.6649
1579.8206
1142.6295
2411.0084
1798.9692
1798.9651
1798.9651
1423.6873
1662.8366
1921.1037
-1.01
0.19
0.17
-0.48
2.12
-0.05
0.33
1.04
-0.23
0.70
3.89
-1.34
0.76
-0.24
1.65
-3.06
3.50
0.60
-0.05
-1.21
-0.42
-0.55
-2.44
-0.27
-1.04
3.61
0.29
-0.43
0
0
0
0
0
0
1
0
1
0
1
1
0
1
1
0
1
1
1
0
0
1
0
0
0
0
0
1
18
18
18
18
18
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
16
16
35
8.3
4
10
2.9
13
15
19
23
18
22
29
21
17
24
21
46
29
25
20
29
4.6
18
18
18
24
30
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
WTMALAK
LLQQANSVGIGPR
YFIIR
KPIIVPEDAK
SCPECSATR + Carbamidomethyl (C)
QPSWPNK
NTSLMSGVYSIVLDIDKK
IDNDGTVVPK
QAMVEGQQLKK
LLDKPLFTPDANTR
LAAEDAGARCVVVR + Carbamidomethyl (C)
ADYEKELANPK
QFYVFNDVDAK
GSFKDSLIK
CANSICNGASAINFKSAK + 2 Acrylamide (C)
NEISQMFASLR + Oxidation (M)
TYGKAVGTH
DELTKNGIASK
MATHITIDKEK + Oxidation (M)
VPQLEIVPNSAEER
DAVLGNNVTIK
STENTEDIQGCDSDKHGWYR + Acrylamide (C)
IGLFSFGGGYAMLPLIK + Oxidation (M)
LCCALQVMSLEK + Acrylamide (C); Oxidation (M)
ALQTFDVSDVWGIGR
IVEIPGEVPPGKYVVVAR
84
591
421
430
185
696
715
763
121
642
114
345
131
93
429.2110
805.9365
655.3226
661.3354
505.2143
961.5578
654.0324
737.7062
466.2311
857.4075
455.7129
410.9063
473.7636
435.7738
856.4074
1609.8584
1308.6306
1320.6562
1008.4140
1921.1010
1959.0754
2210.0968
930.4476
1712.8004
909.4112
1229.6971
945.5126
869.5330
856.4079
1609.8576
1308.6310
1320.6561
1008.4157
1921.1037
1959.0789
2210.0967
930.4480
1712.8048
909.4113
1229.6993
945.5131
869.5334
-0.53
0.51
-0.28
0.09
-1.66
-1.37
-1.80
0.02
-0.43
-2.56
-0.09
-1.78
-0.47
-0.46
0
1
0
0
0
1
1
0
1
1
0
1
1
0
16
16
16
16
16
16
16
16
16
16
16
15
15
15
10
16
24
35
6.2
3.5
14
31
10
25
17
20
42
37
1
1
1
1
1
1
1
1
1
1
1
1
1
1
QAYSNFK
VAALAAGGHFDPAKTGK
GDTDGYVLGISGR
YSEDLQPQTLK
CSDFCHLK + Carbamidomethyl (C)
IVEIPGEVPPGKYVVVAR
QLVEIKEVNLDPNPPVR
LGEHNIDVLEGNEQFINAAK
KLSPSECP + Acrylamide (C)
SEMTDLSK
NRQAIVNFIR
KEVESIGGK
VVSLSIPR
267
179
326
467
535
550
362
603
524
556.3166
502.7739
601.8497
689.3105
746.8779
763.4512
625.3176
821.4378
739.8370
1110.6186
1003.5332
1201.6848
1376.6064
1491.7412
1524.8878
1248.6206
1640.8610
1477.6594
1110.6147
1003.5338
1201.6852
1376.6102
1491.7425
1524.8875
1248.6206
1640.8603
1477.6606
3.51
-0.58
-0.33
-2.76
-0.85
0.21
0.06
0.47
-0.77
0
0
1
1
1
0
1
1
0
15
15
15
15
15
15
15
15
15
22
58
24
15
44
6.8
43
40
22
1
1
1
1
1
1
1
1
1
FIVMDVFLK
EVDLYLPR
KMIAIQQNIK + Oxidation (M)
QNNRQMSDEQK
DVMRAADDLVMLK + Oxidation (M)
LEKPLSVGQNTLVK
EIMLNNEMKK
FFVAPFPEVFGKEK
IMENQSEELEEK
12/9/2009
Page 19 of 22
583
402
607
111
792
230
600
653
668
542
654
534.2944
641.8488
828.4092
453.7209
1085.1880
531.2334
820.4718
879.4572
892.4935
755.8639
879.4574
1599.8614
1281.6830
1654.8038
905.4272
3252.5422
1060.4522
1638.9290
1756.8998
1782.9724
1509.7132
1756.9002
1599.8620
1281.6830
1654.7984
905.4290
3252.5431
1060.4536
1638.9305
1756.8995
1782.9702
1509.7133
1756.8995
-0.42
0.07
3.27
-1.92
-0.28
-1.23
-0.87
0.18
1.25
-0.07
0.41
0
1
1
1
1
0
1
0
0
0
0
15
15
15
15
14
14
14
14
14
14
14
35
29
42
59
32
11
13
42
24
38
39
1
1
1
1
1
1
1
1
1
1
1
LLDKPLFTPDANTR
RLFEAIGHPDK
NEISKTCFEIAASSR
ARGWCSR + Acrylamide (C)
IAGEVPEKDSWYMQTGQGMLATINAAAEQK + Oxidation (M)
TDASFDMFK
KVPQVSTPTLVEVSR
LYPIANGDNQSPIDIK
DVAMKPPSVYLLPPTR
GSSGSSGIVMADWLK + Oxidation (M)
LYPIANGDNQSPIDIK
624
665
109
44
625
528
629
99
159
33
633
728
471
664
842.9003
887.9526
451.7254
403.7294
843.4623
743.8962
563.3174
441.7395
493.2871
396.2187
848.9797
663.3542
691.8162
589.9444
1683.7860
1773.8906
901.4362
805.4442
1684.9100
1485.7778
1686.9304
881.4644
984.5596
790.4228
1695.9448
1987.0408
1381.6178
1766.8114
1683.7886
1773.8971
901.4368
805.4446
1684.9108
1485.7722
1686.9305
881.4647
984.5604
790.4198
1695.9407
1987.0414
1381.6217
1766.8179
-1.53
-3.63
-0.59
-0.47
-0.44
3.83
-0.06
-0.26
-0.73
3.84
2.46
-0.34
-2.80
-3.68
1
0
0
1
0
1
1
1
1
0
0
1
1
0
14
14
14
14
14
14
14
14
14
14
14
13
13
13
45
52
32
64
32
46
24
15
50
45
17
43
36
39
1
1
1
1
1
1
1
1
1
1
1
1
1
1
QDVEALWLEGMTLAAK
FSVQMFK + Oxidation (M)
IKGAFDR
VNGAAISQGVQTTKPSK
GEICVLLKNNNNR
TGVDIKWTAVGTGAALK
KEAYFPK
ALNSPEVKK
TAHHAVR
ISVELIAPVALEEGTR
AILDNFDEYKTLHPALK
EMDQSEALGKMK + Oxidation (M)
MQSTSSPGMINISADTK
739
92
134
429
661
704
794
290
564
378
186
124
234
434
742
142
100
237
741
490
477
77
482
527
658
613
324
310
690.0214
435.2762
475.7288
660.3190
883.8958
965.4530
818.1425
382.5557
783.8733
422.8749
505.2690
467.7309
532.7956
665.3252
1035.0550
480.7504
441.7396
534.2764
690.3724
708.3561
696.3039
426.2346
701.8193
494.9275
880.9670
555.9609
598.8353
584.2983
2067.0424
868.5378
949.4430
1318.6234
1765.7770
1928.8914
3268.5409
1144.6453
1565.7320
1265.6029
1008.5234
933.4472
1063.5766
1328.6358
2068.0954
959.4862
881.4646
1066.5382
2068.0954
1414.6976
1390.5932
850.4546
1401.6240
1481.7607
1759.9194
1664.8609
1195.6560
1166.5820
2067.0459
868.5382
949.4426
1318.6234
1765.7802
1928.8898
3268.5380
1144.6465
1565.7283
1265.6040
1008.5240
933.4477
1063.5775
1328.6361
2068.0953
959.4865
881.4647
1066.5342
2068.0993
1414.6955
1390.5969
850.4549
1401.6293
1481.7595
1759.9217
1664.8594
1195.6561
1166.5819
-1.71
-0.40
0.43
0.05
-1.77
0.88
0.89
-1.10
2.39
-0.92
-0.53
-0.51
-0.76
-0.16
0.08
-0.27
-0.07
3.81
-1.89
1.52
-2.63
-0.26
-3.78
0.78
-1.27
0.87
-0.03
0.13
1
1
0
0
1
1
1
0
1
0
1
0
0
1
0
0
0
1
1
0
0
1
0
1
1
1
0
0
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
12
12
12
12
12
12
12
12
12
57
14
37
47
26
48
42
45
47
61
49
53
52
44
38
85
48
67
40
57
18
84
33
71
68
73
42
71
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
YLSNGLVQCSWTLNKVDK
VIPGDKIK
NDLTLDML + Oxidation (M)
WFEDYYVLGK
WECVSGSGTKNSNQNR
DMNDNEIEAIRIHSEK + Oxidation (M)
IAGEVPEKDSWYMQTGQGMLATINAAAEQK + 2 Oxidation (M)
VIVIGSHAHGR
ALETHTYFER
ETLASYGRL
VIGDHGITPR
VNGEYKVSDYR
IIAVEPEASPVLSGGNPGPHK
VYFFTQR
VGTFYAPK
RCGAYVAATR
ILWLQENEPEVWSAVKK
WAVIPTYQYMK + Oxidation (M)
SRPDMSMEPDAR
AKGFDVSK
MTASTSSTPSTSTK + Oxidation (M)
MRQLAGHPQVVCK + Oxidation (M)
SSNFPILDRSAVEAVR
LARGGGLENGGPSVEPR
EGVLLVAPDGAR
TEQSYAEIVK
385
575
725
149
416
767
710
29
253
268
359
374
492
60
636.3606
533.2999
992.0273
485.7819
436.5662
737.7067
972.9593
385.2032
549.2560
556.3167
623.2897
632.7744
709.3696
417.7288
1270.7066
1596.8779
1982.0400
969.5492
1306.6768
2210.0983
1943.9040
768.3918
1096.4974
1110.6188
1244.5648
1263.5342
1416.7246
833.4430
1270.7067
1596.8797
1982.0473
969.5495
1306.6802
2210.0967
1943.9056
768.3919
1096.5005
1110.6185
1244.5642
1263.5336
1416.7282
833.4429
-0.07
-1.13
-3.64
-0.23
-2.65
0.70
-0.79
-0.02
-2.80
0.27
0.55
0.54
-2.54
0.18
0
0
0
1
1
0
1
1
0
1
0
0
0
0
12
12
12
12
12
12
12
12
12
12
12
12
12
12
44
42
50
39
83
71
54
19
53
49
39
18
90
1.2e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
SDKPVVLVGAMR
VVPSEDTVPLIMGLK
FIENVELLSHLANIGDAK
KLAADVEPK
TDLESKLTCLK + Carbamidomethyl (C)
LGEHNIDVLEGNEQFINAAK
CDMWAQMARTLNIFTK + Oxidation (M)
EKGWHI
LGTMMTGGAAR + 2 Oxidation (M)
SLALFYNKR
DVNGMCISAHK + Acrylamide (C)
TCRPEEECR + 2 Acrylamide (C)
QLEAIGVENMVAK + Oxidation (M)
AAVSALMR + Oxidation (M)
138
602
451
707
372
557
286
588
279
478.2534
547.9602
679.3590
970.9706
630.2974
776.3719
571.8604
802.4484
563.7855
954.4922
1640.8588
1356.7034
1939.9266
1258.5802
1550.7292
1141.7062
1602.8822
1125.5564
954.4957
1640.8603
1356.7038
1939.9237
1258.5798
1550.7320
1141.7070
1602.8842
1125.5600
-3.58
-0.92
-0.25
1.52
0.34
-1.78
-0.69
-1.20
-3.19
0
1
1
0
0
0
1
1
1
12
12
11
11
11
11
11
11
11
50
75
88
85
52
92
14
62
90
1
1
1
1
1
1
1
1
1
FFVAPFPEVFGKEK
GVTFTDRGTYIK
AFDNCILTIEAYDPDIK
HIMMSSVDPAR + Oxidation (M)
SCLLAVSVDDAMEK + Acrylamide (C)
KQTALVELLK
DLARIHAQLGTPSPK
MYRNTSNLK
12/9/2009
Page 20 of 22
209
759
747
361
612
692
555
472
261
500
526
515.2532
731.0112
1046.0280
416.2397
555.6205
632.6481
773.8790
691.8436
553.2632
714.3690
740.8924
1028.4918
2190.0118
2090.0414
1245.6973
1663.8397
1894.9225
1545.7434
1381.6726
1104.5118
1426.7234
1479.7702
1028.4927
2190.0052
2090.0466
1245.6969
1663.8430
1894.9272
1545.7423
1381.6772
1104.5130
1426.7238
1479.7681
-0.85
3.02
-2.47
0.32
-2.03
-2.52
0.72
-3.31
-1.03
-0.27
1.42
1
0
1
0
1
1
0
0
0
0
0
11
11
11
11
11
11
11
11
11
11
11
49
64
83
68
1e+002
93
79
89
76
89
86
1
1
1
1
1
1
1
1
1
1
1
RTDGTFGLY
QWAALMTGDETYAGSLSFSR
MTAGRAYEAETLAHEVITK
LTDPYLVAINK
SPQPWPRATPSTSPR
TQAGSLGAVGYDDEGVKTK
GLEVAPQADGGFETR
NDIQMLIGGEHR
MLMVHQSML + Oxidation (M)
DLLPANSVNPMTR
TAIDAHDADIALVR
254
295
566
424
622
544
123
173
717
446
703
574
507
403
549.3035
574.7763
788.3863
656.8499
840.8818
755.8921
467.2447
499.3109
981.9612
676.8275
964.9620
798.3813
723.8668
642.3406
1096.5924
1147.5380
1574.7580
1311.6852
1679.7490
1509.7696
932.4748
996.6072
1961.9078
1351.6404
1927.9094
1594.7480
1445.7190
1282.6666
1096.5924
1147.5397
1574.7626
1311.6895
1679.7539
1509.7722
932.4750
996.6080
1961.9088
1351.6442
1927.9058
1594.7517
1445.7184
1282.6638
0.06
-1.44
-2.86
-3.22
-2.90
-1.67
-0.12
-0.76
-0.47
-2.78
1.91
-2.30
0.45
2.19
0
0
0
0
1
1
0
0
1
0
1
1
1
1
11
11
11
11
11
11
10
10
10
10
10
10
10
10
51
63
88
79
56
86
2.1e+002
27
78
2.7e+002
91
90
1.1e+002
90
1
1
1
1
1
1
1
1
1
1
1
1
1
1
RPCVAGGRPGK
ILTDEDEWK
MFFLQMSGFPVAGK + Oxidation (M)
NNNQLIAAGEIR
QYEEDGINEWNKR
LNQVRLMNTSYR + Oxidation (M)
GCGITVQQK
LIGAGIVAGAR
RCCGPFDVVLDPSAAGEAR
ICDFGVSGQLEK + Carbamidomethyl (C)
KMIETANGHLNNDGATDK
DVCVGITEKMCLER
MPGIIASEETKDR
GMPMVTLHGKGR
573
531
716
627
441
25
381
411
139
777
497
426
442
758
781
605
433
609
105
689
772
205
473
545
756
281
293
771
797.8699
497.2842
654.0337
563.3041
450.2657
376.7000
634.2761
650.3695
478.7359
802.7133
712.8862
438.8941
675.8492
1096.0130
808.0446
551.9408
664.8333
832.4086
448.7180
622.0011
764.7307
512.2403
692.3087
504.9227
1088.0150
564.2774
574.2831
761.7313
1593.7252
1488.8308
1959.0793
1686.8905
1347.7753
751.3854
1266.5376
1298.7244
955.4572
2405.1181
1423.7578
1313.6605
1349.6838
2190.0114
2421.1120
1652.8006
1327.6520
1662.8026
895.4214
1862.9815
2291.1703
1022.4660
1382.6028
1511.7463
2174.0154
1126.5402
1146.5516
2282.1721
1593.7239
1488.8260
1959.0789
1686.8941
1347.7762
751.3864
1266.5373
1298.7194
955.4545
2405.1169
1423.7572
1313.6575
1349.6835
2190.0052
2421.1118
1652.8006
1327.6568
1662.7963
895.4222
1862.9811
2291.1654
1022.4637
1382.5992
1511.7508
2174.0235
1126.5441
1146.5525
2282.1641
0.83
3.21
0.19
-2.13
-0.65
-1.33
0.28
3.87
2.85
0.49
0.45
2.25
0.22
2.87
0.07
-0.02
-3.56
3.81
-0.82
0.18
2.14
2.28
2.63
-2.97
-3.72
-3.40
-0.77
3.48
0
1
1
0
1
0
0
0
0
1
1
0
0
0
1
1
1
0
0
1
0
1
0
0
0
0
0
1
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
9
9
9
9
9
9
9
9
76
52
42
90
31
1.4e+002
40
95
61
94
82
1e+002
1.4e+002
83
88
1.2e+002
1e+002
1.9e+002
1.3e+002
85
1.2e+002
1.2e+002
95
1.6e+002
1.1e+002
1.1e+002
1.2e+002
1.3e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
ANMTETVRPGGGNMK + 2 Oxidation (M)
SSISIRLSSDGVLR
QLVEIKEVNLDPNPPVR
VIEASPYGQLLVDQR
KLNISYEILQK
EHSIVPA
DMWSGSSCVAPK
QTVLEGVVEAVR
IAAACGEHK + Carbamidomethyl (C)
GDAGMKPSTEGVADYLSHNESKL
IDVYRTGAGFGIR
TGPQLTEAAAAER
NVEKPVQMMFK
WSMSADDPYPQPVVGLAEGR + Oxidation (M)
GDAGMKPSTEGVADYLSHNESKL + Oxidation (M)
TNTTGVDTFKSPNSGK
RGGCLPFGAHSR + Acrylamide (C)
DAAEASFLIAAMFYK + Oxidation (M)
MPPDAPPR + Oxidation (M)
IAYTSNEPMPTAALLKK + Oxidation (M)
MALVSQPSFLCLHTTLQTSAK + Oxidation (M)
RSCVEACK + Acrylamide (C); Carbamidomethyl (C)
MMIEAQSECLR + Carbamidomethyl (C); Oxidation (M)
TADGSEAFTPYLIK
LTQDTLQSSEQMMVVFSSK + Oxidation (M)
MALLHCQSTK + Oxidation (M)
EAVGIPIANVKVDETEDLNEK
635
91
476
68
495
450
789
649
754
508
455
669
744
369
566.6387
434.7315
695.8455
421.7710
711.3766
453.2417
945.4648
581.6171
723.0737
723.8672
681.3563
598.6592
693.0009
627.3533
1696.8943
867.4484
1389.6764
841.5274
1420.7386
1356.7033
2833.3726
1741.8295
2166.1993
1445.7198
1360.6980
1792.9558
2075.9809
1252.6920
1696.8930
867.4491
1389.6810
841.5273
1420.7384
1356.7078
2833.3754
1741.8235
2166.1983
1445.7184
1360.6946
1792.9570
2075.9800
1252.6962
0.74
-0.70
-3.25
0.20
0.17
-3.31
-1.00
3.43
0.44
1.00
2.51
-0.71
0.43
-3.28
1
0
0
0
1
0
0
0
0
1
1
0
1
1
9
9
9
9
9
9
9
9
9
9
9
9
9
9
1.2e+002
1.1e+002
1.7e+002
75
1.6e+002
1.7e+002
1.3e+002
1.4e+002
38
1.7e+002
2.2e+002
1e+002
1.5e+002
93
1
1
1
1
1
1
1
1
1
1
1
1
1
1
QISECVSLQKQLHK + Carbamidomethyl (C)
DFAAAFVK
LVEVEATLCDNK + Carbamidomethyl (C)
ILLIGADK
FCGIEIKIENGK + Acrylamide (C)
IYAYLSDPFIR
TFGVATGPFGAIIAWCIAGGGMYMLAR + Acrylamide (C); 2 Oxidation (M)
SCKPMLSSCIGVVMK + Acrylamide (C); Carbamidomethyl (C); 2 Oxidation (M)
LLCLVAALSLLAGFGHAPTSR + Carbamidomethyl (C)
MPGIIASEETKDR
SRNLSDNISDLK
IAASLNVGQSTISEYLK
EGTYYTQADITSNPKNFK
MLLTHPSAQKK
745
278
554
330
210
670
23
449
463
1042.0080
563.7852
771.3792
603.3356
515.2533
897.9108
376.2378
679.3564
684.8435
2082.0014
1125.5558
1540.7438
1204.6566
1028.4920
1793.8070
750.4610
1356.6982
1367.6724
2081.9973
1125.5601
1540.7410
1204.6564
1028.4927
1793.8142
750.4640
1356.6972
1367.6721
2.00
-3.74
1.87
0.19
-0.66
-3.97
-3.88
0.76
0.23
1
0
0
0
1
0
1
0
0
8
8
8
8
8
8
8
8
8
1.5e+002
1.7e+002
1.8e+002
1.6e+002
1.3e+002
1.8e+002
1.1e+002
1.4e+002
2.1e+002
1
1
1
1
1
1
1
1
1
GTMEDITSIQETNKMLQK + Oxidation (M)
VVHEACIER + Acrylamide (C)
TEFDVVLTDFGGNK
HAVDDLGLPLR
RTDGTFGLY
SGPGVEYNEIVCDVGEK
TYKIVK
EPLMFPAGRPAR + Oxidation (M)
TVTGAYNDTIWK
12/9/2009
Page 21 of 22
571
484
242
364
384
743
733
636
255
280
75
794.8636
469.9130
539.3191
625.7663
635.2742
693.0001
1006.4900
566.6387
550.2450
564.2770
425.7607
1587.7126
1406.7172
1076.6236
1249.5180
1268.5338
2075.9785
2010.9654
1696.8943
1098.4754
1126.5394
849.5068
1587.7086
1406.7194
1076.6230
1249.5179
1268.5377
2075.9800
2010.9647
1696.8969
1098.4798
1126.5416
849.5072
2.54
-1.59
0.63
0.08
-3.03
-0.73
0.39
-1.52
-3.93
-1.89
-0.43
0
0
1
0
0
1
0
1
1
1
1
8
8
8
8
8
8
8
8
7
7
7
90
3.2e+002
1.2e+002
35
62
1.7e+002
2.1e+002
1.6e+002
54
92
1.1e+002
1
1
1
1
1
1
1
1
1
1
1
HEDDTLAIMAEAEK + Oxidation (M)
LSPTQFPDLYAR
IAGIKVDYAK
CEAGGEGLNGMR + Carbamidomethyl (C)
TTMPNAGTSDMK + Oxidation (M)
EIKDDEVTVDFNHPYAGK
IEDFALASDTGYVAQNAAR
DGSANVQQRALLQAAR
CKVSSNSSGCK
MFWAMRTGK
LIRYGTK
650
166
78
323
713
309
498
54
493
691
565
445
708
761
581.6174
496.7212
428.2614
598.3435
651.6470
584.2979
713.3222
414.7617
709.8162
936.9828
787.3110
676.8198
970.9709
733.3513
1741.8304
991.4278
854.5082
1194.6724
1951.9192
1166.5812
1424.6298
827.5088
1417.6178
1871.9510
1572.6074
1351.6250
1939.9272
2197.0321
1741.8311
991.4247
854.5086
1194.6721
1951.9145
1166.5792
1424.6250
827.5116
1417.6184
1871.9490
1572.6041
1351.6231
1939.9230
2197.0361
-0.44
3.18
-0.44
0.31
2.38
1.72
3.37
-3.36
-0.36
1.12
2.13
1.47
2.17
-1.83
1
0
1
1
1
0
1
1
0
1
0
0
1
1
7
7
7
7
7
7
7
7
7
7
7
7
7
7
1.8e+002
59
79
1.1e+002
1.8e+002
1.5e+002
1.6e+002
1.5e+002
1.1e+002
2.2e+002
14
2.2e+002
2.9e+002
2.2e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
WATSEEGQKFIGDFK
AQETGGADFP
AVRSAVPR
GKVTAHIEVGGK
KTSQMGHIMGSHTWSHK
TIHTNGQPGSR
TKFMLYCNSMR + 2 Oxidation (M)
LVLELKN
CSESTEFEALFR
RAYAEVQGLVDADPNVR
GMCQTGDCGGVLECK + Carbamidomethyl (C); Oxidation (M)
NPDINWAMFTK + Oxidation (M)
CAKSASASTLEEAMEVVK + Acrylamide (C); Oxidation (M)
YSPIDDLLYRNCEPAETK + Acrylamide (C)
561
623
95
608
693
749
690
795
748
82
39
688
712
400
621
423
620
187
370
480
335
232
705
368
750
52
604
581
778.3596
840.8820
436.7748
831.3929
632.6487
697.6884
936.4979
1169.8280
697.6880
428.7780
399.1929
929.9445
975.9656
640.8826
840.8817
656.7882
840.8812
505.2691
627.7690
699.2880
610.7986
532.7620
645.6772
627.2764
697.6888
412.7529
824.4128
800.3898
1554.7046
1679.7494
871.5350
1660.7712
1894.9243
2090.0434
1870.9812
3506.4622
2090.0422
855.5414
796.3712
1857.8744
1949.9166
1279.7506
1679.7488
1311.5618
1679.7478
1008.5236
1253.5234
1396.5614
1219.5826
1063.5094
1934.0098
1252.5382
2090.0446
823.4912
1646.8110
1598.7650
1554.7024
1679.7542
871.5378
1660.7775
1894.9184
2090.0500
1870.9788
3506.4703
2090.0466
855.5430
796.3715
1857.8679
1949.9153
1279.7500
1679.7526
1311.5587
1679.7495
1008.5240
1253.5194
1396.5612
1219.5873
1063.5080
1934.0044
1252.5428
2090.0500
823.4916
1646.8073
1598.7657
1.42
-2.82
-3.21
-3.78
3.11
-3.17
1.29
-2.31
-2.13
-1.76
-0.36
3.51
0.70
0.52
-2.25
2.37
-0.96
-0.34
3.23
0.15
-3.84
1.32
2.78
-3.60
-2.59
-0.40
2.31
-0.41
0
1
1
0
1
0
1
0
1
0
0
0
0
0
0
0
1
1
0
0
0
1
1
0
1
0
0
0
7
6
6
6
6
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
1.7e+002
1.5e+002
1.3e+002
2.2e+002
3e+002
3.1e+002
2.3e+002
36
3e+002
80
1.4e+002
1e+003
2.8e+002
64
1.9e+002
1.2e+002
1.8e+002
3e+002
1.1e+002
43
4e+002
2.8e+002
2.8e+002
1.6e+002
3.8e+002
1.7e+002
5.2e+002
3e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
FCNDLQLSPDEFK
VEGRMGPSACGTCIR + Acrylamide (C); Carbamidomethyl (C); Oxidation (M)
ELKGILSL
WYAMVGTAMSMIIR + 2 Oxidation (M)
IEFMTFMGAFKMWLK + Oxidation (M)
IACETLATTNCVVIAGEVR + Acrylamide (C); Carbamidomethyl (C)
YKVPQLEIVPNSAEER
MTDETYALLCDFYELTMSNGYFINGFCDK + Acrylamide (C); 2 Oxidation (M)
DWASDLQIIIDVAMSKGGR + Oxidation (M)
LLVLVDGK
THTNPLD
TSNGEIFHMDSLTAAHK
QLDLTELSGCFAASSSHK + Carbamidomethyl (C)
SVVNSGPIVIAPK
DDGSEVELTAEPGSFK
SDYCGLLCPER + Carbamidomethyl (C)
ETMGGLGEKEDVMPR + 2 Oxidation (M)
ETLASYGRL
EVAATMSGDDSR + Oxidation (M)
MHDTHPGDMGQR + Oxidation (M)
ALDDPHYVYK
MRGAGTNDVK + Oxidation (M)
AGGHALPCVVDVRDEVAVK
MMDETQETLR
QKGCEIPIIALTASAMTGDR + Oxidation (M)
VAKPGAGPK
DENSEECLLLLIEK
SACLECGHAAVLAGR + 2 Acrylamide (C)
553
736
774
770
755
775
760
590
784
534
783
738
682
461
769.3243
1021.5030
769.6685
761.7303
725.6784
802.7117
731.3927
805.9363
870.7744
746.4114
837.7528
1029.5010
912.9159
682.8484
1536.6340
2040.9914
2305.9837
2282.1691
2174.0134
2405.1133
2191.1563
1609.8580
2609.3014
1490.8082
2510.2366
2056.9874
1823.8172
1363.6822
1536.6401
2040.9972
2305.9880
2282.1650
2174.0208
2405.1081
2191.1597
1609.8616
2609.2990
1490.8093
2510.2450
2056.9888
1823.8108
1363.6772
-3.91
-2.83
-1.86
1.77
-3.42
2.13
-1.56
-2.23
0.90
-0.69
-3.37
-0.64
3.52
3.70
0
0
1
1
1
0
1
1
1
1
1
1
0
1
5
5
4
4
4
4
4
4
4
4
4
4
4
4
58
4e+002
1.2e+002
3.7e+002
3.4e+002
3.4e+002
1.5e+003
2.6e+002
3.9e+002
3.5e+002
4.1e+002
4.1e+002
5e+002
5e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
GNTQDETGSQSNSGR
LLMEGCPDPGNISQIDIAR
FKCLYCYNYDLCSECVAK + 2 Acrylamide (C)
QMVQTYLVEDVAVCSLTQKK
QADIDSGLCPSIPMASNSGRR
ESQQESNALDPSVDSTLEESIK
IRQTPPAPAATGVGAVSQDLDK
QGFAAAYFELPKLR
MAKTSWKPGNMIYPLPAVMVTCR + Oxidation (M)
VQIFGSLKAATDNK
SDMWSLGVILFQMNTGRLPWK + 2 Oxidation (M)
IEDDMVYNDLYQRLVR + Oxidation (M)
NQNNQCGIATASSYPTV + Carbamidomethyl (C)
EWLGVEFRISE
714
791
790
746
785
641
788
276
727
976.9717
973.4694
968.1384
697.6874
875.4474
857.3854
921.7053
560.8340
992.0284
1951.9288
2917.3864
2901.3934
2090.0404
2623.3204
1712.7562
2762.0941
1119.6534
1982.0422
1951.9363
2917.3859
2901.3823
2090.0387
2623.3251
1712.7498
2762.1040
1119.6539
1982.0477
-3.82
0.16
3.81
0.78
-1.79
3.75
-3.61
-0.42
-2.73
1
1
1
0
1
0
1
0
0
3
3
3
3
3
2
2
2
2
5e+002
4.2e+002
5.1e+002
6e+002
5e+002
3.7e+002
37
1.2e+002
4.2e+002
1
1
1
1
1
1
1
1
1
CFYGWQLTVNEHTKR + Acrylamide (C)
YSINYIPLGCCRLSWNCANGMILR + 2 Acrylamide (C); Oxidation (M)
ECFGMRPEMTGVLVSRINPLSDAYK + Carbamidomethyl (C); 2 Oxidation (M)
IEVVIANNDAMAMGAVEALK + 2 Oxidation (M)
NPIPMCGVPHRAVNSYIDVLIDK + Carbamidomethyl (C); Oxidation (M)
IGVPEPQPAVASGCGCD + 2 Carbamidomethyl (C)
NKEEWTVDDCTECTCQNSATVCR + Acrylamide (C); Carbamidomethyl (C)
YAIISEALLK
IFALPAIGIGMLMTMLMK + 2 Oxidation (M)
12/9/2009
780
559
768
107
106
786
730
24
722
6
9
808.0426
777.8373
738.6830
450.2747
449.7713
875.4476
1000.5570
376.2378
656.2956
354.2250
357.6368
2421.1060
1553.6600
2213.0272
898.5348
897.5280
2623.3210
1999.0994
750.4610
1965.8650
706.4354
713.2590
10
13
15
27
37
38
47
76
102
108
116
129
144
165
357.6369
362.2222
362.7382
384.2350
398.2508
398.2508
406.2485
425.7607
442.2767
450.7904
458.7877
472.2879
480.8007
494.3005
713.2592
722.4298
723.4618
766.4554
794.4870
794.4870
810.4824
849.5068
882.5388
899.5662
915.5608
942.5612
959.5868
986.5864
180
250
483
610
634
721
502.8141
545.2003
702.2764
832.4258
849.3873
983.9371
1003.6136
1088.3860
1402.5382
1662.8370
1696.7600
1965.8596
2421.1053
1553.6636
2213.0279
898.5348
897.5284
2623.3172
1999.1023
750.4640
1965.8659
0.29
-2.29
-0.34
0.03
-0.37
1.43
-1.43
-3.88
-0.49
1
1
1
1
0
1
0
1
0
2
2
2
2
1
1
1
1
0
4.7e+002
2.1e+002
5.7e+002
8.7e+002
4.8e+002
7e+002
3.7e+002
6.1e+002
4.7e+002
Page 22 of 22
1
1
1
1
1
1
1
1
1
MGLERAALECGTGEEGTAWIDGR
CRDICEDALMNGK + Acrylamide (C); Oxidation (M)
LCVNDQQVGVFCDNVTKTCK
GGKNALLAR
VVLHTTTK
AISRCLVVMLSLGWGVTCDTLGDK + Acrylamide (C); Oxidation (M)
ELISLGMGAEANQILSLIK
TYKIVK
ADEEDIAALVIDNGSGMCK + Oxidation (M)
Search Parameters
Type of search
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
MS/MS Ion Search
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
795
12/9/2009
Page 1 of 26
User
Email
Search title
MS data file
Database
Timestamp
Protein hits
:
:
:
:
:
:
:
LTQFT Daemon
D:\Data\Mark\18_7-09\N_18_7_09_8.RAW
9 Dec 2009 at 04:11:20 GMT
gi|28317
gi|157165101 nlpa lipoprotein [Campylobacter concisus 13826]
gi|158604975 D-methionine-binding lipoprotein MetQ [Campylobacter concisus 13826]
gi|157164251 holo-[acyl-carrier-protein] synthase [Campylobacter concisus 13826]
gi|181402
gi|157164012 2-cys peroxiredoxin BAS1, (Thiol-specific antioxidant protein) [Campylobacter concisus 13826]
gi|6320157
gi|157165339 glutamine-binding periplasmic protein/glutamine transport system permease protein [Campylobacter concisus 13826]
gi|291474
gi|157164883 enolase (2-phosphoglycerate dehydratase; 2-phospho-D-glycerate hydro-lyase) [Campylobacter concisus 13826]
gi|122411
gi|1942778
gi|122614
gi|157165684 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase [Campylobacter concisus 13826]
gi|157165429 phosphoribosylaminoimidazole-succinocarboxamide synthase [Campylobacter concisus 13826]
gi|136429
gi|1351907
gi|225632
casein alphaS1
gi|157163886 thiol peroxidase [Campylobacter concisus 13826]
gi|27807521 prostaglandin D2 synthase, brain [Bos taurus]
gi|291462
gi|291480
gi|255322305 D-methionine-binding lipoprotein MetQ [Campylobacter showae RM3277]
gi|149721278 PREDICTED: similar to anhydrase II,carbonic [Equus caballus]
gi|291468
gi|34069
gi|223570
gi|157165151 HDOD domain-contain protein [Campylobacter concisus 13826]
gi|149716548 PREDICTED: similar to Apolipoprotein A-I precursor (Apo-AI) (ApoA-I) [Equus caballus]
gi|157164741 adenylate kinase [Campylobacter concisus 13826]
gi|157164034 uridylate kinase [Campylobacter concisus 13826]
gi|27806963 casein alpha-S2 [Bos taurus]
gi|57167806 probable oxidoreductase Cj0807 [Campylobacter coli RM2228]
gi|162811
kappa-casein precursor
gi|157164768 molybdate ABC transporter, periplasmic molybdate-binding protein [Campylobacter concisus 13826]
gi|154175458 ProP protein [Campylobacter curvus 525.92]
gi|494583
gi|2232299
gi|157165617 NAD-dependent epimerase/dehydratase family protein [Campylobacter concisus 13826]
gi|126742337 hemoglobin alpha chain [Ochotona curzoniae]
gi|538275
gi|157165298 cytochrome c oxidase, diheme subunit, membrane-bound [Campylobacter concisus 13826]
gi|157164484 3-ketoacyl-(acyl-carrier-protein) reductase [Campylobacter concisus 13826]
gi|3928489
gi|157165622 integrase/recombinase [Campylobacter concisus 13826]
gi|157164291 response regulator receiver domain-containing protein [Campylobacter concisus 13826]
gi|157165615 adenylosuccinate synthetase (imp--aspartate ligase; adss; ampsase) [Campylobacter concisus 13826]
gi|157164911 putative periplasmic protein [Campylobacter concisus 13826]
gi|146296629 NLPA lipoprotein [Caldicellulosiruptor saccharolyticus DSM 8903]
gi|157164695 elongation factor P [Campylobacter concisus 13826]
gi|149721282 PREDICTED: similar to Carbonic anhydrase 1 (Carbonic anhydrase I) (Carbonate dehydratase I) (CA-I) [Equus caballus]
gi|154173631 periplasmic nitrate reductase, diheme cytochrome c subunit [Campylobacter curvus 525.92]
gi|27806907 clusterin [Bos taurus]
gi|3914346
12/9/2009
Page 2 of 26
107
3
2.80 %
197
3
1.52 %
Format As
Standard scoring
Show sub-sets 0
Require bold red
Show pop-ups
Select All
1.
Help
Peptide Summary
Select None
MudPIT scoring
Suppress pop-ups
Search Selected
Mass: 59492
Score: 827
gi|28317
Error tolerant
Queries matched: 18
Archive Report
emPAI: 0.71
Query
35
166
198
220
235
250
254
298
321
350
386
484
493
521
558
559
715
863
Observed
404.2032
502.2801
516.3026
532.8083
545.7691
553.7665
555.2484
583.2958
601.3116
617.8430
631.8024
691.3276
695.8442
717.8883
498.5829
747.3710
854.3894
998.9891
Mr(expt)
806.3918
1002.5456
1030.5906
1063.6020
1089.5236
1105.5184
1108.4822
1164.5770
1200.6086
1233.6714
1261.5902
1380.6406
1389.6738
1433.7620
1492.7269
1492.7274
1706.7642
1995.9636
Mr(calc)
806.3923
1002.5458
1030.5910
1063.6026
1089.5237
1105.5186
1108.4825
1164.5775
1200.6098
1233.6717
1261.5899
1380.6408
1389.6736
1433.7626
1492.7270
1492.7270
1706.7649
1995.9636
ppm Miss Score
Expect Rank
-0.51
0
23
5
1
-0.13
1
25
2.9
1
-0.36
0
52
0.0032
1
-0.49
1
32
0.4
1
-0.03
0
48
0.018
1
-0.14
0
(34)
0.44
1
-0.24
0
44
0.028
1
-0.36
0
52
0.0087
1
-1.00
0
49
0.011
1
-0.20
1
35
0.33
1
0.31
0
62
0.00059
1
-0.14
0
75 4.4e-005
1
0.21
0
76 2.4e-005
1
-0.41
1
27
1.6
1
-0.07
1
(22)
5.9
1
0.32
1
46
0.024
1
-0.39
0
130 5.4e-011
1
0.01
0
49
0.016
1
Peptide
R.LAADDFR.L
K.SEITELRR.N
R.VLDELTLTK.A
R.LASYLDKVR.A
K.VTMQNLNDR.L
K.VTMQNLNDR.L + Oxidation (M)
K.DAEAWFNEK.S
R.LENEIQTYR.S
R.QSVEADINGLR.R
R.LKYENEVALR.Q
R.SLLEGEGSSGGGGR.G
R.ALEESNYELEGK.I
K.QSLEASLAETEGR.Y
K.IRLENEIQTYR.S
R.SQYEQLAEQNRK.D
R.SQYEQLAEQNRK.D
K.ELTTEIDNNIEQISSYK.S
gi|21961605
Mass: 58792
Score: 827
Queries matched: 18
gi|114667509
Mass: 62761
Score: 827
Queries matched: 18
gi|114667511
Mass: 58244
Score: 827
Queries matched: 18
gi|114667513
Mass: 56692
Score: 827
Queries matched: 18
Mass: 63308
Score: 827
Queries matched: 18
gi|119581085
gi|195972866
Mass: 58766
Score: 827
Queries matched: 18
gi|251757513
Mass: 58818
Score: 827
Queries matched: 18
gi|114667507
Mass: 100282
Score: 823
Queries matched: 18
2.
gi|157164859
Mass: 27476
Score: 769
Queries matched: 22
emPAI: 2.52
12/9/2009
Query
44
75
78
126
176
322
388
389
414
452
482
607
608
637
726
727
737
738
796
802
803
811
3.
Observed
412.2150
429.7681
433.2423
479.2555
507.8188
601.3246
632.8299
632.8306
639.8326
665.3719
690.3435
786.3939
786.3943
803.3972
860.4528
860.4530
867.4449
868.4505
924.5002
622.0008
932.4978
945.9407
Mr(expt)
822.4154
857.5216
864.4700
956.4964
1013.6230
1200.6346
1263.6452
1263.6466
1277.6506
1328.7292
1378.6724
1570.7732
1570.7740
1604.7798
1718.8910
1718.8914
1732.8752
1734.8864
1846.9858
1862.9806
1862.9810
1889.8668
Mr(calc)
822.4157
857.5222
864.4705
956.4967
1013.6233
1200.6350
1263.6459
1263.6459
1277.6503
1328.7299
1378.6728
1570.7739
1570.7739
1604.7794
1718.8913
1718.8913
1732.8744
1734.8862
1846.9862
1862.9811
1862.9811
1889.8683
ppm Miss Score
Expect Rank
-0.28
0
27
2
1
-0.65
0
28
1.6
2
-0.53
1
27
2.9
1
-0.28
1
51
0.0087
1
-0.25
1
49
0.0073
1
-0.28
0
65
0.00035
1
-0.54
0
(65) 0.00043
1
0.56
0
66
0.00034
1
0.25
0
35
0.49
1
-0.52
1
50
0.0083
1
-0.28
1
56
0.0035
1
-0.44
0
(48)
0.021
1
0.07
0
85 3.6e-006
1
0.25
0
71
9e-005
1
-0.13
0
(60)
0.0011
1
0.11
0
(70) 0.00012
1
0.49
1
34
0.49
1
0.15
0
73 5.4e-005
1
-0.20
1
29
1.1
1
-0.30
1
(24)
3.6
1
-0.05
1
(23)
4.9
1
-0.79
0
24
3.4
1
Page 3 of 26
Peptide
K.ISEISMK.Y + Oxidation (M)
K.GVELIISK.R
K.SFADLKGK.K
K.YFGKDISK.K.GVELIISKR.A
K.GNTELLEAINK.A
K.NGATVVVADGFSK.G
K.NGATVVVADGFSK.G
K.GELVGYDVDIAK.A
K.KGNTELLEAINK.A
K.DNNDIKSFADLK.G
K.SVHSATSNWAAIAEK.N
K.SVHSATSNWAAIAEK.N
K.ADVVFNQVSINEDR.K
K.ADVVFNQVSINEDRK.K
K.IAYTSNEPMPTAALLKK.G
K.IAYTSNEPMPTAALLKK.G + Oxidation (M)
K.IAYTSNEPMPTAALLKK.G + Oxidation (M)
R.ADDTINDNVTFFDYIK.Q
gi|158605017
Mass: 30046
Score: 669
Queries matched: 26
emPAI: 1.85
Query
10
26
36
76
132
153
169
224
394
437
445
628
629
630
631
632
633
642
643
697
744
780
782
787
978
979
4.
Observed
363.6946
398.2033
404.7317
432.7448
485.2610
496.7923
504.2740
537.8005
633.3478
654.3454
657.8381
799.8973
799.8978
534.2943
800.9379
534.2944
800.9384
807.8950
807.8950
844.4724
871.9230
605.6292
907.9410
914.9462
1085.1900
1090.5200
Mr(expt)
725.3746
794.3920
807.4488
863.4750
968.5074
991.5700
1006.5334
1073.5864
1264.6810
1306.6762
1313.6616
1597.7800
1597.7810
1599.8611
1599.8612
1599.8614
1599.8622
1613.7754
1613.7754
1686.9302
1741.8314
1813.8658
1813.8674
1827.8778
3252.5482
3268.5382
gi|11935049
Mass: 66027
Mr(calc)
725.3748
794.3923
807.4490
863.4752
968.5080
991.5702
1006.5335
1073.5869
1264.6815
1306.6768
1313.6615
1597.7810
1597.7810
1599.8620
1599.8620
1599.8620
1599.8620
1613.7759
1613.7759
1686.9305
1741.8311
1813.8669
1813.8669
1827.8825
3252.5431
3268.5380
ppm Miss Score
Expect Rank
-0.25
0
26
0.38
1
-0.30
0
29
2.1
1
-0.23
1
24
5.9
1
-0.23
0
28
2.5
4
-0.53
1
35
0.37
1
-0.16
1
45
0.028
1
-0.04
1
41
0.094
1
-0.46
0
31
1.4
1
-0.40
0
41
0.074
1
-0.46
1
36
0.28
1
0.09
1
47
0.022
1
-0.61
0
(48)
0.016
1
0.02
0
71 7.6e-005
1
-0.61
0
(26)
3
1
-0.50
0
(50)
0.01
1
-0.42
0
(23)
5
1
0.13
0
53
0.0046
1
-0.30
0
(59)
0.0014
1
-0.30
0
(47)
0.021
1
-0.14
1
47
0.011
1
0.17
1
20
9.9
1
-0.61
0
(44)
0.041
1
0.31
0
45
0.037
1
-2.56
0
(35)
0.32
1
1.57
1
(31)
0.67
1
0.05
1
50
0.0081
1
Score: 645
Queries matched: 15
Peptide
K.FIGDFK.L
K.GVTFTDR.G
K.SIADKFK.G
K.QSFELIK.K
K.DGIKFFSR.G
K.QSFELIKK.D
K.VEKEFVEK.G
K.WTAVGTGAALK.L
K.NFYSLIAVNPK.H
K.TDIENAEKFIK.W
R.GTYIKYEANQK.G
R.NAVMYNDFVVIADK.S
R.NAVMYNDFVVIADK.S
K.LLDKPLFTPDANTR.K
K.LLDKPLFTPDANTR.K
K.LLDKPLFTPDANTR.K
K.LLDKPLFTPDANTR.K
K.WATSEEGQKFIGDFK.L
K.LGEDCNADILFVHSPK.V + Carbamidomethyl (C)
K.IAGEVPEKDSWYMQTGQGMLATINAAAEQK.G + Oxidation (M)
K.IAGEVPEKDSWYMQTGQGMLATINAAAEQK.G + 2 Oxidation (M)
emPAI: 0.40
Query
49
83
134
177
201
221
434
454
487
495
496
545
Observed
416.7478
437.7531
487.2693
508.2237
517.2615
533.2640
651.8607
670.8386
692.3484
465.2488
697.3699
738.3961
Mr(expt)
831.4810
873.4916
972.5240
1014.4328
1032.5084
1064.5134
1301.7068
1339.6626
1382.6822
1392.7246
1392.7252
1474.7776
Mr(calc)
831.4814
873.4920
972.5240
1014.4328
1032.5087
1064.5138
1301.7078
1339.6619
1382.6830
1392.7249
1392.7249
1474.7780
ppm Miss Score
Expect Rank
-0.42
0
32
0.59
4
-0.37
0
33
1.3
1
0.08
0
49
0.023
1
0.03
0
27
0.57
1
-0.27
0
40
0.097
1
-0.34
0
26
4
1
-0.75
0
70 7.9e-005
1
0.55
1
59
0.0013
1
-0.55
0
24
4.7
1
-0.21
1
(24)
4.6
1
0.27
1
51
0.0088
1
-0.23
0
56
0.0028
1
Peptide
K.SISISVAR.G
R.SLVNLGGSK.S
K.IEISELNR.V
K.DVDGAYMTK.V + Oxidation (M)
R.TLLEGEESR.M
K.AQYEDIAQK.S
R.SLDLDSIIAEVK.A
K.SKAEAESLYQSK.Y
K.SLNNQFASFIDK.V
R.TNAENEFVTIKK.D
R.TNAENEFVTIKK.D
R.FLEQQNQVLQTK.W
12/9/2009
662
724
757
829.4001
858.9303
883.3714
1656.7856
1715.8460
1764.7282
1656.7856
1715.8438
1764.7275
0.03
1.30
0.43
gi|119395750
Mass: 65999
Score: 645
Mass: 65450
Score: 645
gi|160961491
5.
0
0
0
41
93
45
0.074
6.3e-007
0.01
1
1
1
Page 4 of 26
R.SGGGFSSGSAGIINYQR.R
K.QISNLQQSISDAEQR.G
R.FSSCGGGGGSFGAGGGFGSR.S + Carbamidomethyl (C)
Queries matched: 15
Queries matched: 15
gi|157164888
Mass: 27581
Score: 466
Queries matched: 15
emPAI: 0.77
Query
40
78
98
118
119
130
195
296
297
403
482
623
677
678
729
6.
Observed
407.7482
433.2423
455.7587
474.7628
474.7629
482.7599
515.7637
583.2593
583.2596
637.3265
690.3435
796.8997
833.4379
555.9611
860.9473
Mr(expt)
813.4818
864.4700
909.5028
947.5110
947.5112
963.5052
1029.5128
1164.5040
1164.5046
1272.6384
1378.6724
1591.7848
1664.8612
1664.8615
1719.8800
Mr(calc)
813.4821
864.4705
909.5032
947.5110
947.5110
963.5059
1029.5131
1164.5047
1164.5047
1272.6390
1378.6728
1591.7842
1664.8621
1664.8621
1719.8792
ppm Miss Score
Expect Rank
-0.29
1
34
0.74
2
-0.53
1
27
2.9
1
-0.37
0
21
7.1
1
0.08
0
(42)
0.13
2
0.29
0
(30)
1.9
2
-0.67
0
46
0.044
1
-0.25
1
32
2.7
1
-0.57
0
(26)
1.6
1
-0.06
0
65
0.00023
1
-0.46
0
51
0.0081
1
-0.28
1
56
0.0035
1
0.40
0
80 1.2e-005
1
-0.50
1
(23)
6.2
1
-0.36
1
32
0.74
1
0.51
1
23
6.3
1
gi|157165101
Mass: 28322
Score: 466
Queries matched: 17
nlpa lipoprotein [Campylobacter concisus 13826]
Peptide
R.RVDAVVR.D
K.SFADLKGK.K
K.ERPNAPVK.I
K.SMELLISR.R
K.SMELLISR.R
K.SMELLISR.R + Oxidation (M)
K.SYFGKDVSK.K.DADSATSNWAK.V
K.DADSATSNWAK.V
R.DNIVFYDFIK.E
K.DNNDIKSFADLK.G
K.ADVVFNQVSITDER.K
K.ADVVFNQVSITDERK.K
emPAI: 1.18
Query
17
127
189
190
319
320
344
406
432
638
639
640
852
853
854
899
900
7.
Observed
382.2265
480.7717
512.2789
512.2790
600.3532
400.5713
614.8422
638.8173
650.3674
538.6262
807.4358
807.4359
658.3732
658.3738
987.0571
1044.5520
696.7040
Mr(expt)
762.4384
959.5288
1022.5432
1022.5434
1198.6918
1198.6921
1227.6698
1275.6200
1298.7202
1612.8568
1612.8570
1612.8572
1972.0978
1972.0996
1972.0996
2087.0894
2087.0902
Mr(calc)
762.4388
959.5288
1022.5437
1022.5437
1198.6921
1198.6921
1227.6711
1275.6207
1298.7194
1612.8573
1612.8573
1612.8573
1972.0993
1972.0993
1972.0993
2087.0898
2087.0898
ppm Miss Score
Expect Rank
-0.48
0
32
1.2
1
0.09
0
37
0.32
1
-0.41
1
35
0.38
1
-0.22
1
(35)
0.39
1
-0.22
1
35
0.2
1
-0.03
1
(22)
4.7
1
-0.99
0
56
0.002
1
-0.54
0
35
0.39
1
0.65
1
50
0.0098
1
-0.32
0
(55)
0.003
1
-0.15
0
(43)
0.053
1
-0.02
0
60
0.00087
1
-0.78
1
(26)
0.76
1
0.13
1
(35)
0.11
1
0.17
1
39
0.04
1
-0.19
0
86 1.9e-006
1
0.16
0
(50)
0.0097
1
Peptide
K.NFIITR.Y
K.VATVNDITK.N
R.YKGEVIPAF.R.YKGEVIPAF.K.AGLIEIDKNVK.V
K.AGLIEIDKNVK.V
K.ALVDAVLSPDTK.N
K.VAIAYDPSNGNR.A
K.VATVNDITKNPK.N
K.NLQFVELEGAQIPR.T
K.NLQFVELEGAQIPR.T
K.NLQFVELEGAQIPR.T
K.ALVDAVLSPDTKNFIITR.Y
K.DALLLEDANSPYANIIVTR.A
K.DALLLEDANSPYANIIVTR.A
gi|114667176
Mass: 107386
Score: 437
Queries matched: 11
emPAI: 0.16
Query
35
217
220
235
250
287
288
349
358
767
961
8.
Observed
404.2032
530.7853
532.8083
545.7691
553.7665
579.2988
579.2989
616.8022
618.2676
896.3676
837.3822
Mr(expt)
806.3918
1059.5560
1063.6020
1089.5236
1105.5184
1156.5830
1156.5832
1231.5898
1234.5206
1790.7206
2509.1248
Mr(calc)
806.3923
1059.5560
1063.6026
1089.5237
1105.5186
1156.5836
1156.5836
1231.5906
1234.5215
1790.7205
2509.1245
ppm Miss Score
Expect Rank
-0.51
0
23
5
1
0.02
0
28
2.6
1
-0.49
1
32
0.4
1
-0.03
0
48
0.018
1
-0.14
0
(34)
0.44
1
-0.52
0
61
0.00069
1
-0.35
0
(22)
5.6
1
-0.58
0
55
0.0032
1
-0.65
0
76 6.3e-006
1
0.09
0
65 4.1e-005
1
0.12
0
48
0.011
1
Peptide
R.LAADDFR.T
K.TLLDIDNTR.M
R.LASYLDKVR.A
K.VTMQNLNDR.L
K.VTMQNLNDR.L + Oxidation (M)
R.QGVDADINGLR.Q
R.QGVDADINGLR.Q
R.FSSSSGYGGGSSR.V
K.EIETYHNLLEGGQEDFESSGAGK.I
gi|158604975
Mass: 28432
Score: 403
Queries matched: 12
emPAI: 0.94
D-methionine-binding lipoprotein MetQ [Campylobacter concisus 13826]
Query
Observed
Mr(expt)
Mr(calc)
ppm
Peptide
12/9/2009
9.
114
131
255
362
417
577
578
590
591
618
681
469.7450
483.2576
556.3032
620.3509
641.8373
758.8949
758.8951
766.8923
511.5973
792.4267
835.9028
937.4754
964.5006
1110.5918
1238.6872
1281.6600
1515.7752
1515.7756
1531.7700
1531.7701
1582.8388
1669.7910
937.4756
964.5018
1110.5921
1238.6870
1281.6605
1515.7756
1515.7756
1531.7705
1531.7705
1582.8395
1669.7907
-0.21
-1.20
-0.22
0.17
-0.36
-0.20
0.06
-0.28
-0.26
-0.42
0.18
0
0
0
1
0
0
0
0
0
1
0
888
1021.0140
2040.0134
2040.0123
0.54
1
49
0.011
32
0.99
53
0.003
52 0.0032
41
0.077
48
0.023
(26)
3.4
(31)
1
(20)
12
37
0.15
43
0.051
48
0.028
Page 5 of 26
1
1
1
1
1
1
1
1
1
1
1
K.DGYTLEIK.E
K.YNGAIIPAF.K.TPLDIVDNPK.K
K.TPLDIVDNPKK.L
K.DSPYVNYVVVK.A
K.TVGVHLEPMGVYSK.K
K.TVGVHLEPMGVYSK.K
K.TVGVHLEPMGVYSK.K + Oxidation (M)
K.FIETKYNGAIIPAF.K.DGAVVSIPNDPTNESR.A
1
K.ELKDGAVVSIPNDPTNESR.A
gi|157164251
Mass: 29645
Score: 373
Queries matched: 14
emPAI: 0.53
holo-[acyl-carrier-protein] synthase [Campylobacter concisus 13826]
Query
14
43
133
143
187
231
244
246
533
534
538
867
868
949
10.
Observed
376.2208
410.7498
486.2794
491.7637
511.2873
543.8111
367.2202
550.3271
729.8466
729.8467
733.8685
1002.0250
668.3527
803.0773
Mr(expt)
750.4270
819.4850
970.5442
981.5128
1020.5600
1085.6076
1098.6388
1098.6396
1457.6786
1457.6788
1465.7224
2002.0354
2002.0363
2406.2101
Mr(calc)
750.4276
819.4854
970.5447
981.5131
1020.5604
1085.6080
1098.6397
1098.6397
1457.6787
1457.6787
1465.7235
2002.0371
2002.0371
2406.2100
-0.67
1
22
12
6
-0.46
1
28
1.5
1
-0.49
0
27
2.5
1
-0.25
0
26
1.6
1
-0.31
1
48
0.024
1
-0.37
0
64 0.00053
1
-0.83
1
(24)
3.7
1
-0.03
1
35
0.36
1
-0.01
1
29
0.86
1
0.13
1
(29)
0.91
1
-0.70
0
34
0.57
1
-0.82
0
39
0.12
1
-0.41
0
(30)
1
1
0.01
1
22
7.1
1
gi|181402
Mass: 65825
Score: 353
Queries matched: 6
Peptide
K.AKIEYK.R.KLFDGIK.V
R.IIDDLINR.K
R.HILVEDEK.T
K.KFAELASQK.S
K.TANDIIAQLK.N
R.IIDDLINRK.L
R.IIDDLINRK.L
K.VSDSDLRDFYNK.N
K.VSDSDLRDFYNK.N
K.AAQEGIAVELYMR.K + Oxidation (M)
K.AAGTVSFEQAKPEIEQAVKMEK.F + Oxidation (M)
emPAI: 0.22
Query
134
251
382
451
535
545
Observed
487.2693
554.2752
627.8074
665.3668
730.9035
738.3961
Mr(expt)
972.5240
1106.5358
1253.6002
1328.7190
1459.7924
1474.7776
Mr(calc)
972.5240
1106.5356
1253.6001
1328.7187
1459.7922
1474.7780
ppm Miss Score
Expect Rank
0.08
0
49
0.023
1
0.23
0
44
0.058
1
0.16
0
86 2.4e-006
1
0.25
0
58
0.0017
1
0.16
0
59
0.0011
1
-0.23
0
56
0.0028
1
Peptide
K.IEISELNR.V
K.AQYEEIAQR.S
R.GFSSGSAVVSGGSR.R
R.NLDLDSIIAEVK.A
K.VDLLNQEIEFLK.V
R.FLEQQNQVLQTK.W
gi|47132620
Mass: 65393
Score: 353
Queries matched: 6
Mass: 65944
Score: 353
Queries matched: 6
gi|148727309
11.
gi|157164012
Mass: 22011
Score: 324
Queries matched: 17
emPAI: 0.76
12.
Query
87
94
116
186
326
327
328
407
408
409
410
Observed
445.7235
453.7210
472.7271
511.2726
602.8434
602.8437
402.2316
426.2406
426.2407
638.8577
638.8577
Mr(expt)
889.4324
905.4274
943.4396
1020.5306
1203.6722
1203.6728
1203.6730
1275.7000
1275.7003
1275.7008
1275.7008
Mr(calc)
889.4327
905.4276
943.4399
1020.5314
1203.6724
1203.6724
1203.6724
1275.7009
1275.7009
1275.7009
1275.7009
ppm Miss Score
-0.31
0
24
-0.22
0
(22)
-0.29
1
36
-0.74
0
33
-0.12
0
(40)
0.38
0
44
0.48
0
(38)
-0.75
1
(23)
-0.51
1
(30)
-0.06
1
(34)
-0.06
1
(39)
411
426
439
543
544
946
638.8578
646.8547
654.3514
492.2677
737.8984
802.7128
1275.7010
1291.6948
1306.6882
1473.7813
1473.7822
2405.1166
1275.7009
1291.6958
1306.6881
1473.7827
1473.7827
2405.1169
0.09
-0.77
0.09
-1.00
-0.34
-0.13
gi|6320157
Mass: 19051
Score: 320
1
1
1
0
0
1
Expect Rank
5.7
1
12
1
0.18
1
0.44
1
0.083
1
0.034
1
0.12
1
3.8
1
0.79
1
0.32
1
0.098
1
40
0.085
(34)
0.37
40
0.11
(38)
0.23
78 1.8e-005
29
1.1
Queries matched: 25
1
1
1
1
1
1
Peptide
R.NIDEMIR.M
R.NIDEMIR.M + Oxidation (M)
R.YDEFKSR.G
R.FPIVADMTK.S
R.HAVINDLPLGR.N
R.HAVINDLPLGR.N
R.HAVINDLPLGR.N
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
K.VRFPIVADMTK.S
R.GSFLLDKDGTVR.H
R.GFDVLLEDAGVALR.G
R.GFDVLLEDAGVALR.G
K.GDAGMKPSTEGVADYLSHNESKL.-
emPAI: 1.25
12/9/2009
Page 6 of 26
Query
1
2
11
351
352
353
354
355
356
357
371
372
373
374
469
470
472
473
824
825
827
828
829
830
848
Observed
353.7156
353.7159
365.2288
412.2393
617.8555
617.8556
617.8557
617.8557
412.2396
617.8558
625.3298
625.3300
625.3301
625.3303
681.9028
454.9377
681.9032
681.9033
961.5587
641.3749
641.3751
961.5591
641.3752
961.5593
980.5468
Mr(expt)
705.4166
705.4172
728.4430
1233.6961
1233.6964
1233.6966
1233.6968
1233.6968
1233.6970
1233.6970
1248.6450
1248.6454
1248.6456
1248.6460
1361.7910
1361.7913
1361.7918
1361.7920
1921.1028
1921.1029
1921.1035
1921.1036
1921.1038
1921.1040
1959.0790
Mr(calc)
705.4174
705.4174
728.4432
1233.6969
1233.6969
1233.6969
1233.6969
1233.6969
1233.6969
1233.6969
1248.6462
1248.6462
1248.6462
1248.6462
1361.7918
1361.7918
1361.7918
1361.7918
1921.1037
1921.1037
1921.1037
1921.1037
1921.1037
1921.1037
1959.0789
ppm Miss Score
Expect Rank
-1.01
0
(30)
0.96
4
-0.16
0
33
0.56
4
-0.24
0
39
0.26
1
-0.66
0
(23)
2.5
1
-0.35
0
69 6.7e-005
1
-0.19
0
(53)
0.0029
1
-0.03
0
(52)
0.0037
1
-0.03
0
(60) 0.00058
1
0.07
0
(20)
5.7
1
0.13
0
(44)
0.026
1
-0.95
0
(28)
2.1
1
-0.63
0
43
0.071
1
-0.47
0
(21)
9.8
1
-0.15
0
(31)
1.2
1
-0.58
1
(23)
1.2
1
-0.42
1
(29)
0.34
1
0.01
1
45
0.007
1
0.15
1
(43)
0.012
1
-0.43
1
(28)
0.23
1
-0.42
1
62 8.1e-005
1
-0.11
1
(27)
0.31
1
-0.02
1
(44)
0.0057
1
0.05
1
(28)
0.24
1
0.19
1
(37)
0.034
1
0.07
1
30
0.49
1
Peptide
K.YVVVAR.A
K.YVVVAR.A
K.QLVEIK.E
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.EVNLDPNPPVR.G
K.EVNLDPNPPVR.G
K.EVNLDPNPPVR.G
K.EVNLDPNPPVR.G
K.KIVEIPGEVPPGK.Y
K.KIVEIPGEVPPGK.Y
K.KIVEIPGEVPPGK.Y
K.KIVEIPGEVPPGK.Y
K.QLVEIKEVNLDPNPPVR.G
gi|151941952
Mass: 19021
Score: 320
Queries matched: 25
gi|190405057
Mass: 19007
Score: 320
Queries matched: 25
13.
gi|157165339
Mass: 28771
Score: 313
Queries matched: 7
emPAI: 0.55
glutamine-binding periplasmic protein/glutamine transport system permease protein [Campylobacter concisus 13826]
Query
78
126
260
482
623
729
741
14.
Observed
433.2423
479.2555
558.3062
690.3435
796.8997
860.9473
868.9421
Mr(expt)
864.4700
956.4964
1114.5978
1378.6724
1591.7848
1719.8800
1735.8696
Mr(calc)
864.4705
956.4967
1114.5982
1378.6728
1591.7842
1719.8792
1735.8702
ppm Miss Score
Expect Rank
-0.53
1
27
2.9
1
-0.28
1
51
0.0087
1
-0.31
0
34
0.55
1
-0.28
1
56
0.0035
1
0.40
0
80 1.2e-005
1
0.51
1
23
6.3
1
-0.32
0
44
0.046
1
gi|115495209
Mass: 59194
Score: 284
Queries matched: 14
Peptide
K.SFADLKGK.R
K.YFGKDISK.K.INNALNELSK.E
K.DNNDIKSFADLK.G
K.ADVVFNQVSITDER.K
K.ADVVFNQVSITDERK.K
K.IIEAGDEPMYTAAIVK.K + Oxidation (M)
emPAI: 0.24
Query
12
34
86
291
308
375
376
390
391
420
733
945
952
953
15.
Observed
367.2338
403.7113
443.7280
580.7900
587.7975
626.2780
626.2784
633.2859
633.2867
642.7742
865.3610
800.0045
804.6758
804.6761
Mr(expt)
732.4530
805.4080
885.4414
1159.5654
1173.5804
1250.5414
1250.5422
1264.5572
1264.5588
1283.5338
1728.7074
2396.9917
2411.0056
2411.0065
gi|291474
Mass: 17423
Mr(calc)
732.4534
805.4083
885.4419
1159.5656
1173.5812
1250.5424
1250.5424
1264.5580
1264.5580
1283.5340
1728.7083
2396.9928
2411.0084
2411.0084
Score: 267
-0.48
0
41
0.027
1
-0.29
0
22
5.6
1
-0.47
0
36
0.11
1
-0.09
0
48
0.018
1
-0.63
0
(21)
8.5
1
-0.75
0
(36)
0.16
1
-0.11
0
37
0.13
1
-0.62
0
(23)
3.8
1
0.65
0
(34)
0.28
1
-0.09
0
32
0.2
1
-0.50
1
25
0.56
1
-0.46
1
(28)
0.27
1
-1.18
1
(37)
0.04
1
-0.80
1
44 0.0089
1
Queries matched: 12
Peptide
R.FNLVLK.N
R.FVGDGGVR.M
R.FSVQMFK.F
R.YCTDPATAEDK.C + Acrylamide (C)
R.STENTEDIQGCDSDKHGWYR.F + Carbamidomethyl (C)
emPAI: 0.43
Query
6
7
Observed
360.6872
360.6873
Mr(expt)
719.3598
719.3600
Mr(calc)
719.3602
719.3602
ppm Miss Score
-0.53
0
29
-0.25
0
(29)
Expect Rank
0.51
1
0.52
1
Peptide
R.TSAQWK.S
R.TSAQWK.S
12/9/2009
16.
Page 7 of 26
102
108
196
197
274
289
331
546
547
459.2233
466.2313
516.2610
516.2613
572.7690
579.7766
604.3068
738.8679
738.8683
916.4320
930.4480
1030.5074
1030.5080
1143.5234
1157.5386
1206.5990
1475.7212
1475.7220
916.4324
930.4480
1030.5083
1030.5083
1143.5230
1157.5387
1206.5993
1475.7216
1475.7216
-0.39
0.00
-0.86
-0.27
0.37
-0.02
-0.21
-0.26
0.28
1
1
0
0
0
0
1
0
0
26
0.79
(24)
1.4
(32)
0.77
57
0.0029
31
0.46
(31)
0.5
34
0.5
(68)
0.0002
89 1.4e-006
1
1
1
1
2
1
1
1
1
K.KLSPSECP.- + Carbamidomethyl (C)
K.KLSPSECP.- + Acrylamide (C)
K.YAASSYLTR.T
K.YAASSYLTR.T
K.SYSSVSCQVK.H + Carbamidomethyl (C)
R.ASGVPDRFSGSK.S
K.VNDAVTTDGVQTTR.S
K.VNDAVTTDGVQTTR.S
548
738.8688
1475.7230
1475.7216
0.96
0
(64)
1
K.VNDAVTTDGVQTTR.S
0.00044
gi|157164883
Mass: 24731
Score: 255
Queries matched: 9
emPAI: 0.29
enolase (2-phosphoglycerate dehydratase; 2-phospho-D-glycerate hydro-lyase) [Campylobacter concisus 13826]
Query
16
111
112
149
225
442
443
567
579
17.
Observed
381.7289
468.7299
468.7301
494.2539
538.7554
657.3403
657.3406
753.8863
761.8840
Mr(expt)
761.4432
935.4452
935.4456
986.4932
1075.4962
1312.6660
1312.6666
1505.7580
1521.7534
Mr(calc)
761.4436
935.4461
935.4461
986.4933
1075.4968
1312.6663
1312.6663
1505.7582
1521.7531
-0.41
0
26
2.5
6
-0.88
0
55 0.0021
1
-0.46
0
(52) 0.0043
1
-0.10
0
47
0.022
1
-0.51
0
27
1.5
1
-0.17
1
40
0.11
1
0.28
1
(28)
1.5
1
-0.08
0
60 0.0011
1
0.24
0
(22)
7.2
1
Peptide
R.LFNAIGK.V
K.AFAGESQAR.N
K.AFAGESQAR.N
K.EAGYHAIAR.H
K.CGNEIEVQK.V + Carbamidomethyl (C)
R.NKYELYGDLAK.E
R.NKYELYGDLAK.E
K.CITENLTAVNLMK.A + Carbamidomethyl (C)
K.CITENLTAVNLMK.A + Carbamidomethyl (C); Oxidation (M)
gi|122411
Mass: 15236
Score: 244
Queries matched: 12
emPAI: 0.50
Query
203
213
214
233
276
277
400
572
573
574
792
793
Observed
521.2753
529.2727
529.2729
544.3166
573.3298
573.3300
634.8584
758.3622
505.9110
758.3629
917.4493
917.4503
Mr(expt)
1040.5360
1056.5308
1056.5312
1086.6186
1144.6450
1144.6454
1267.7022
1514.7098
1514.7112
1514.7112
1832.8840
1832.8860
Mr(calc)
1040.5365
1056.5314
1056.5314
1086.6186
1144.6452
1144.6452
1267.7024
1514.7113
1514.7113
1514.7113
1832.8846
1832.8846
-0.43
0
(37)
0.15
1
-0.54
0
(25)
2.2
1
-0.16
0
43
0.049
1
0.07
1
22
7.8
1
-0.11
1
50
0.0089
1
0.24
1
(31)
0.75
1
-0.10
0
63 0.00032
1
-0.99
0
(28)
1.6
1
-0.12
0
(39)
0.09
2
-0.07
0
44
0.033
1
-0.30
0
(22)
7.6
1
0.79
0
22
7.1
1
Peptide
R.MFLGFPTTK.T
K.LRVDPVNFK.L
M.VLSAADKTNVK.A
M.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
K.TYFPHFDLSHGSAQVK.A
gi|230479
Mass: 15105
Score: 244
Queries matched: 12
Mass: 15105
Score: 244
Queries matched: 12
gi|230632
gi|313500
Mass: 15236
Score: 244
Queries matched: 12
gi|24158717
Mass: 15105
Score: 244
Queries matched: 12
18.
gi|1942778
Mass: 15089
Score: 237
Queries matched: 12
emPAI: 0.84
Query
203
213
214
233
276
277
400
561
562
563
792
793
Observed
521.2753
529.2727
529.2729
544.3166
573.3298
573.3300
634.8584
500.5792
750.3658
750.3661
917.4493
917.4503
Mr(expt)
1040.5360
1056.5308
1056.5312
1086.6186
1144.6450
1144.6454
1267.7022
1498.7158
1498.7170
1498.7176
1832.8840
1832.8860
gi|146149083
Mass:
gi|146149272
Mass:
Mr(calc)
1040.5365
1056.5314
1056.5314
1086.6186
1144.6452
1144.6452
1267.7024
1498.7164
1498.7164
1498.7164
1832.8846
1832.8846
-0.43
0
(37)
0.15
1
-0.54
0
(25)
2.2
1
-0.16
0
43
0.049
1
0.07
1
22
7.8
1
-0.11
1
50
0.0089
1
0.24
1
(31)
0.75
1
-0.10
0
63 0.00032
1
-0.45
0
(33)
0.47
1
0.40
0
(33)
0.45
1
0.80
0
37
0.2
1
-0.30
0
(22)
7.6
1
0.79
0
22
7.1
1
15220
Score: 237
caballus]
15220
Score: 237
Peptide
R.MFLGFPTTK.T
K.LRVDPVNFK.L
-.VLSAADKTNVK.A
-.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEFGAEALER.M
K.VGGHAGEFGAEALER.M
K.VGGHAGEFGAEALER.M
Queries matched: 12
Queries matched: 12
12/9/2009
Page 8 of 26
19.
gi|122614
Mass: 15998
Score: 230
Queries matched: 10
emPAI: 1.16
Query
281
395
404
405
815
864
865
873
874
875
Observed
575.3406
633.4183
637.8664
637.8666
632.6481
1000.4650
1000.4660
672.6435
1008.4620
1008.4630
Mr(expt)
1148.6666
1264.8220
1273.7182
1273.7186
1894.9225
1998.9154
1998.9174
2014.9087
2014.9094
2014.9114
Mr(calc)
1148.6666
1264.8231
1273.7183
1273.7183
1894.9214
1998.9146
1998.9146
2014.9095
2014.9095
2014.9095
ppm Miss Score
Expect Rank
0.06
0
33
0.18
1
-0.82
0
70 2.4e-006
1
-0.03
0
(40)
0.069
1
0.28
0
44
0.03
1
0.59
1
20
10
1
0.43
0
63
0.00036
1
1.43
0
(31)
0.72
1
-0.41
0
(35)
0.21
1
-0.03
0
(51)
0.0057
1
0.97
0
(29)
0.73
1
gi|255683515
Mass: 16129
Score: 230
20.
Peptide
K.VVAGVANALAHK.Y
R.LLGNVLVVVLAR.H
R.LLVVYPWTQR.F
R.LLVVYPWTQR.F
R.HFGKDFTPELQASYQK.V
Queries matched: 10
gi|157165684
Mass: 26183
Score: 230
Queries matched: 9
emPAI: 0.62
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase [Campylobacter concisus 13826]
Query
142
526
536
588
597
627
641
859
860
21.
Observed
491.2481
723.3744
731.3712
766.4315
774.4284
799.4469
807.4445
991.5363
661.3605
Mr(expt)
980.4816
1444.7342
1460.7278
1530.8484
1546.8422
1596.8792
1612.8744
1981.0580
1981.0597
Mr(calc)
980.4815
1444.7344
1460.7293
1530.8480
1546.8429
1596.8797
1612.8746
1981.0592
1981.0592
0.19
0
36
0.28
1
-0.10
0
60 0.0014
1
-1.00
0
(25)
3.9
1
0.32
0
32
0.34
1
-0.41
0
(31)
0.51
1
-0.27
0
(34)
0.24
1
-0.10
0
40
0.091
1
-0.60
0
63 0.0003
1
0.22
0
(39) 0.084
1
Peptide
K.DGSYDALLK.K
K.INGIISAMSATPDR.L
K.INGIISAMSATPDR.L + Oxidation (M)
K.IINMSFDGLIPALK.A
K.IINMSFDGLIPALK.A + Oxidation (M)
K.VVPSEDTVPLIMGLK.V
K.VVPSEDTVPLIMGLK.V + Oxidation (M)
gi|157164686
Mass: 31540
Score: 229
Queries matched: 4
emPAI: 0.35
Query
383
402
415
789
22.
Observed
629.8352
636.3326
640.8275
917.4450
Mr(expt)
1257.6558
1270.6506
1279.6404
1832.8754
Mr(calc)
1257.6564
1270.6517
1279.6408
1832.8752
ppm Miss Score
Expect Rank
-0.47
0
51
0.0093
1
-0.82
0
37
0.18
1
-0.29
0
60
0.0013
1
0.15
0
81 9.2e-006
1
gi|157164773
Mass: 24977
Score: 222
Queries matched: 6
Peptide
K.SLEENLQANLK.L
K.LSNPALSANEQK.G
R.SEIGSTQNAVFK.G
R.SVANANSVENLDSSDIAK.S
emPAI: 0.65
Query
147
280
522
644
648
773
23.
Observed
493.2874
575.3038
718.3754
810.4069
818.4036
900.4547
Mr(expt)
984.5602
1148.5930
1434.7362
1618.7992
1634.7926
1798.8948
Mr(calc)
984.5604
1148.5938
1434.7354
1618.7985
1634.7934
1798.8949
ppm Miss Score
Expect Rank
-0.16
0
27
2.1
1
-0.68
1
29
1.4
1
0.57
0
45
0.04
1
0.48
0
46
0.027
1
-0.46
0
(42)
0.085
1
-0.01
0
74 4.3e-005
1
Peptide
R.GSVVLPAGTGK.V
K.SGQVNFRVDK.Q
K.EQLNENISTFIK.A
K.TGTVTMDVAQAVNNAK.S
K.TGTVTMDVAQAVNNAK.S + Oxidation (M)
K.AAGADIVGADDLVEDIQK.G
gi|157165429
Mass: 26861
Score: 209
Queries matched: 7
phosphoribosylaminoimidazole-succinocarboxamide synthase [Campylobacter concisus 13826]
Query
129
139
207
210
234
273
531
24.
Observed
482.2788
489.7661
523.8015
525.2906
545.2985
567.8167
727.9239
Mr(expt)
962.5430
977.5176
1045.5884
1048.5666
1088.5824
1133.6188
1453.8332
Mr(calc)
962.5437
977.5182
1045.5880
1048.5665
1088.5826
1133.6193
1453.8327
-0.63
0
34
0.37
2
-0.54
0
49
0.025
1
0.45
1
31
0.7
1
0.10
1
21
13
2
-0.11
1
29
1.6
3
-0.38
1
21
8.3
1
0.41
1
24
1.6
1
gi|194676990
Mass: 45494
Score: 206
Queries matched: 6
Peptide
R.EINSILFK.F
K.VAYEEVLR.R
R.NIATGSLSKR.L
R.FRQDLGSVK.V
K.SEKDLQTLR.H
K.VAYEEVLRR.I
K.KCEIIPLEVVVR.N + Carbamidomethyl (C)
emPAI: 0.07
12/9/2009
Query
106
148
155
228
258
337
25.
Observed
465.7662
493.7975
497.8054
540.2956
557.8452
608.3036
Mr(expt)
929.5178
985.5804
993.5962
1078.5766
1113.6758
1214.5926
Mr(calc)
929.5182
985.5808
993.5971
1078.5771
1113.6757
1214.5931
-0.37
0
25
5.7
5
-0.34
0
32
0.76
1
-0.86
0
40
0.025
1
-0.41
0
44
0.034
1
0.10
1
23
2
3
-0.41
0
42
0.051
1
gi|136429
Mass: 24394
Score: 205
Queries matched: 12
Page 9 of 26
Peptide
K.LTPETLTR.W
R.DILSQLGIK.K
K.LAVSQVIHK.A
R.HIDELSLPR.F
R.DILSQLGIKK.I
R.FSISSDYQLR.D
emPAI: 0.67
Query
54
55
56
57
205
206
923
924
925
926
927
932
Observed
421.7580
421.7581
421.7582
421.7582
523.2853
523.2855
737.7064
737.7064
1106.0560
1106.0560
737.7067
1142.0940
Mr(expt)
841.5014
841.5016
841.5018
841.5018
1044.5560
1044.5564
2210.0974
2210.0974
2210.0974
2210.0974
2210.0983
2282.1734
Mr(calc)
841.5022
841.5022
841.5022
841.5022
1044.5564
1044.5564
2210.0967
2210.0967
2210.0967
2210.0967
2210.0967
2282.1729
ppm Miss Score
Expect Rank
-0.84
0
56
0.0044
1
-0.60
0
(38)
0.27
1
-0.36
0
(49)
0.019
1
-0.36
0
(46)
0.042
1
-0.29
0
37
0.24
1
0.09
0
(24)
7
1
0.29
0
(67) 0.00025
1
0.29
0
(36)
0.27
1
0.33
0
87 2.2e-006
1
0.33
0
(67) 0.00023
1
0.70
0
(43)
0.063
1
0.25
0
25
3.3
1
Peptide
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
K.LSSPATLNSR.V
K.LSSPATLNSR.V
gi|494360
Mass: 23458
Score: 205
Queries matched: 12
gi|999627
Mass: 8814
Score: 205
Queries matched: 12
gi|1942351
Mass: 13284
Score: 205
Queries matched: 12
gi|2914482
Mass: 23460
Score: 205
Queries matched: 12
gi|3318722
Mass: 23457
Score: 205
Queries matched: 12
gi|110590762
Mass: 23458
Score: 205
Queries matched: 12
Mass: 24157
Score: 205
Queries matched: 12
gi|157878102
Mass: 25865
Score: 205
Queries matched: 12
gi|242253868
26.
gi|1351907
Mass: 69248
Score: 205
Queries matched: 7
emPAI: 0.10
Query
60
104
294
435
549
Observed
424.2552
464.2502
582.3188
653.3616
740.4014
Mr(expt)
846.4958
926.4858
1162.6230
1304.7086
1478.7882
Mr(calc)
846.4963
926.4861
1162.6234
1304.7088
1478.7881
-0.56
1
33
0.3
1
-0.30
0
26
2
1
-0.27
0
36
0.27
1
-0.14
0
23
5.3
1
0.07
0
48
0.014
1
Peptide
R.LSQKFPK.A
K.YLYEIAR.R
K.LVNELTEFAK.T
K.HLVDEPQNLIK.Q
K.LGEYGFQNALIVR.Y
650
651
820.4723
547.3174
1638.9300
1638.9304
1638.9305
1638.9305
-0.26
-0.07
R.KVPQVSTPTLVEVSR.S
R.KVPQVSTPTLVEVSR.S
1
1
39
(31)
0.041
0.31
1
1
Mass: 69278
Score: 205
Queries matched: 7
gi|30794280
27.
gi|225632
Mass: 24420
casein alphaS1
Score: 202
Queries matched: 8
emPAI: 0.47
Query
399
453
489
490
654
748
749
750
Observed
634.3552
669.3439
692.8680
692.8685
821.4379
880.4759
587.3199
880.4763
Mr(expt)
1266.6958
1336.6732
1383.7214
1383.7224
1640.8612
1758.9372
1758.9379
1758.9380
Mr(calc)
1266.6972
1336.6735
1383.7227
1383.7227
1640.8603
1758.9377
1758.9377
1758.9377
-1.06
0
47
0.017
1
-0.19
1
37
0.23
1
-0.93
0
37
0.18
1
-0.21
0
(34)
0.4
1
0.59
1
40
0.11
1
-0.23
0
(37)
0.19
1
0.12
0
41
0.063
1
0.22
0
(22)
5.1
1
Peptide
R.YLGYLEQLLR.L
K.HIQKEDVPSER.Y
R.FFVAPFPEVFGK.E
R.FFVAPFPEVFGK.E
R.FFVAPFPEVFGKEK.V
K.HQGLPQEVLNENLLR.F
K.HQGLPQEVLNENLLR.F
K.HQGLPQEVLNENLLR.F
12/9/2009
Page 10 of 26
gi|30794348
Mass: 24513
Score: 202
Queries matched: 8
gi|159793187
Mass: 13839
Score: 202
Queries matched: 8
Mass: 23598
Score: 202
Queries matched: 8
gi|159793191
gi|159793193
Mass: 18671
Score: 202
Queries matched: 8
Mass: 23473
Score: 202
Queries matched: 8
gi|159793197
Mass: 22442
Score: 202
Queries matched: 8
gi|159793209
28.
gi|157163886
Mass: 26131
Score: 194
Queries matched: 6
thiol peroxidase [Campylobacter concisus 13826]
emPAI: 0.13
Query
3
99
113
191
242
243
29.
Observed
355.7073
456.2583
469.2711
512.7873
550.3212
550.3218
Mr(expt)
709.4000
910.5020
936.5276
1023.5600
1098.6278
1098.6290
Mr(calc)
709.4010
910.5025
936.5280
1023.5600
1098.6285
1098.6285
ppm Miss Score
Expect Rank
-1.36
0
24
4
1
-0.47
1
25
2.1
1
-0.38
1
36
0.23
1
0.02
1
28
1.1
1
-0.57
0
(20)
6.8
1
0.52
0
81 6.2e-006
1
gi|27807521
Mass: 21216
Score: 186
Queries matched: 6
prostaglandin D2 synthase, brain [Bos taurus]
Peptide
K.SSLIYK.D
K.KYFAAGVR.S
K.SYLAEVKK.E
K.SSLIYKDAK.A
K.VAGDIEAVVVK.L
K.VAGDIEAVVVK.L
emPAI: 0.16
Query
21
52
73
504
505
593
30.
Observed
388.6878
421.2152
429.2129
704.3672
704.3674
768.9194
Mr(expt)
775.3610
840.4158
856.4112
1406.7198
1406.7202
1535.8242
gi|291462
Mass: 20404
Mr(calc)
775.3613
840.4164
856.4113
1406.7194
1406.7194
1535.8235
Score: 182
ppm Miss Score
Expect Rank
-0.34
0
39
0.051
1
-0.60
0
34
0.4
1
-0.03
0
(21)
5.2
1
0.31
1
32
1.1
1
0.60
1
(28)
2.8
1
0.46
0
80 5.7e-006
1
Queries matched: 7
Peptide
R.GPGQDFR.M
R.MATLYSR.S
R.MATLYSR.S + Oxidation (M)
R.AEVKEHFTTFAK.S
R.AEVKEHFTTFAK.S
K.SLGFTEEGIVFLPK.T
emPAI: 1.14
Query
331
556
566
689
690
709
710
31.
Observed
604.3068
746.3464
753.3545
562.6437
843.4627
850.4383
850.4385
Mr(expt)
1206.5990
1490.6782
1504.6944
1684.9093
1684.9108
1698.8620
1698.8624
gi|291480
Mass: 23493
Mr(calc)
1206.5993
1490.6784
1504.6940
1684.9108
1684.9108
1698.8617
1698.8617
Score: 172
-0.21
1
34
0.5
1
-0.09
0
51 0.0056
1
0.29
0
(43)
0.04
1
-0.91
0
(35)
0.26
1
0.03
0
58 0.0013
1
0.21
0
39
0.11
1
0.44
0
(29)
1.4
1
Queries matched: 8
Peptide
R.ASGVPDRFSGSK.S
K.VNGAAISQGVQTTKPSK.Q
K.VNGAAISQGVQTTKPSK.Q
K.YAASSYLTLTPAQWK.S
K.YAASSYLTLTPAQWK.S
emPAI: 0.70
Query
102
108
227
331
556
566
695
696
32.
Observed
459.2233
466.2313
540.2955
604.3068
746.3464
753.3545
844.4518
563.3039
Mr(expt)
916.4320
930.4480
1078.5764
1206.5990
1490.6782
1504.6944
1686.8890
1686.8899
Mr(calc)
916.4324
930.4480
1078.5771
1206.5993
1490.6784
1504.6940
1686.8901
1686.8901
-0.39
1
26
0.79
1
0.00
1
(24)
1.4
1
-0.59
1
22
6.3
1
-0.21
1
34
0.5
1
-0.09
0
51 0.0056
1
0.29
0
(43)
0.04
1
-0.61
0
(37)
0.2
1
-0.12
0
40
0.11
1
Peptide
K.TLIYGNDKR.A
R.ASGVPDRFSGSK.S
gi|157163941
Mass: 27030
Score: 168
Queries matched: 7
emPAI: 0.26
Query
266
324
325
333
334
759
Observed
562.7825
601.7791
601.7792
605.3109
605.3112
883.4243
Mr(expt)
1123.5504
1201.5436
1201.5438
1208.6072
1208.6078
1764.8340
Mr(calc)
1123.5509
1201.5438
1201.5438
1208.6078
1208.6078
1764.8359
-0.45
0
28
1.4
1
-0.09
0
26
1.4
1
0.07
0
(26)
1.5
1
-0.42
0
(41)
0.09
1
0.07
0
67 0.00024
1
-1.04
0
(35)
0.33
1
Peptide
K.FDTDSSIIAR.V
R.SVFADCQSFK.V + Acrylamide (C)
R.SVFADCQSFK.V + Acrylamide (C)
R.VSDIDTVFWK.L
R.VSDIDTVFWK.L
K.TGEVEYYQLPFYTR.V
12/9/2009
760
33.
883.4248
1764.8350
1764.8359
-0.48
gi|157165374
Mass: 33156
Score: 160
0
46
0.027
Page 11 of 26
1
Queries matched: 3
K.TGEVEYYQLPFYTR.V
emPAI: 0.33
34.
Query
488
684
Observed
692.8328
838.3996
Mr(expt)
1383.6510
1674.7846
Mr(calc)
1383.6518
1674.7850
ppm Miss Score
Expect Rank
-0.54
0
85 3.1e-006
1
-0.19
0
32
0.59
1
Peptide
K.DSGLTSDLSSFAGK.K
869
1005.9900
2009.9654
2009.9694
-1.98
0
43
0.052
1
gi|255322305
Mass: 28167
Score: 158
Queries matched: 7
emPAI: 0.25
D-methionine-binding lipoprotein MetQ [Campylobacter showae RM3277]
Query
39
304
305
577
578
590
591
35.
Observed
407.2268
585.8580
585.8582
758.8949
758.8951
766.8923
511.5973
Mr(expt)
812.4390
1169.7014
1169.7018
1515.7752
1515.7756
1531.7700
1531.7701
Mr(calc)
812.4392
1169.7019
1169.7019
1515.7756
1515.7756
1531.7705
1531.7705
-0.18
0
48
0.012
1
-0.43
0
63 0.00018
1
-0.09
0
(45)
0.012
1
-0.20
0
48
0.023
1
0.06
0
(26)
3.4
1
-0.28
0
(31)
1
1
-0.26
0
(20)
12
1
gi|149721278
Mass: 29070
Score: 153
Queries matched: 4
PREDICTED: similar to anhydrase II,carbonic [Equus caballus]
Peptide
K.LNDNPLK.T
R.ALDVLASAGLIK.L
R.ALDVLASAGLIK.L
K.TVGVHLEPMGVYSK.K
K.TVGVHLEPMGVYSK.K
emPAI: 0.24
Query
162
163
303
652
36.
Observed
501.2970
501.7589
585.2645
820.9716
Mr(expt)
1000.5794
1001.5032
1168.5144
1639.9286
Mr(calc)
1000.5805
1001.5029
1168.5149
1639.9298
-1.01
0
46
0.019
1
0.30
0
25
3
1
-0.39
0
42
0.044
1
-0.68
0
40
0.038
1
Peptide
K.VLDVLDSIK.T
R.QSPVDIDTK.A
K.SADFTNFDPR.G
K.AVQQPDGLAVVGVFLK.V
gi|157165180
Mass: 20941
Score: 141
Queries matched: 3
emPAI: 0.35
Query
61
731
831
37.
Observed
424.7657
576.3157
964.4687
Mr(expt)
847.5168
1725.9253
1926.9228
gi|291468
Mass: 23981
Mr(calc)
847.5167
1725.9261
1926.9224
Score: 139
0.15
0
40
0.084
1
-0.48
0
35
0.24
1
0.22
0
66 0.00029
1
Queries matched: 7
Peptide
R.IISVAFAK.T
emPAI: 0.69
Query
102
108
360
556
566
695
696
38.
Observed
459.2233
466.2313
618.3226
746.3464
753.3545
844.4518
563.3039
Mr(expt)
916.4320
930.4480
1234.6306
1490.6782
1504.6944
1686.8890
1686.8899
Mr(calc)
916.4324
930.4480
1234.6306
1490.6784
1504.6940
1686.8901
1686.8901
-0.39
1
26
0.79
1
0.00
1
(24)
1.4
1
0.03
1
22
8.1
1
-0.09
0
51 0.0056
1
0.29
0
(43)
0.04
1
-0.61
0
(37)
0.2
1
-0.12
0
40
0.11
1
Peptide
R.VSGVPDRFSGSK.S
gi|157165403
Mass: 22036
Score: 137
Queries matched: 3
emPAI: 0.33
Query
261
366
783
39.
Observed
559.3343
622.8458
912.4240
Mr(expt)
1116.6540
1243.6770
1822.8334
Mr(calc)
1116.6543
1243.6772
1822.8333
-0.21
0
48 0.0089
3
-0.12
0
42
0.063
1
0.08
0
46
0.019
1
Peptide
K.FLGALLQVEK.K
R.AGSGEVGINLLSK.F
K.NQNIEFSLESNGNETK.I
gi|27807517
Mass: 42533
Score: 135
Queries matched: 5
emPAI: 0.16
Query
97
174
194
310
Observed
454.7453
507.7190
515.7163
590.3602
Mr(expt)
907.4760
1013.4234
1029.4180
1178.7058
Mr(calc)
907.4763
1013.4236
1029.4186
1178.7063
-0.30
0
32
0.94
1
-0.19
0
53 0.0013
1
-0.50
0
(39) 0.018
1
-0.40
0
50 0.0017
1
Peptide
R.DTQYLLR.T
R.ADFSGMSSGR.G
R.ADFSGMSSGR.G + Oxidation (M)
R.LVLVNAIYFK.G
12/9/2009
311
40.
590.3605
1178.7064
1178.7063
gi|34069
Mass: 39195
Score: 132
0.11
0
(42)
0.011
Queries matched: 3
1
Page 12 of 26
R.LVLVNAIYFK.G
emPAI: 0.08
Query
180
251
451
Observed
508.7724
554.2752
665.3668
Mr(expt)
1015.5302
1106.5358
1328.7190
Mr(calc)
1015.5298
1106.5356
1328.7187
0.43
0
30
1.9
1
0.23
0
44
0.058
1
0.25
0
58 0.0017
1
Peptide
R.QLDSIVGER.G
K.AQYEEIAQR.S
R.NLDLDSIIAEVK.A
gi|386849
Mass: 59916
Score: 132
Queries matched: 3
keratin type II [Homo sapiens]
gi|908790
Mass: 59962
Score: 132
Queries matched: 3
gi|908801
Mass: 60163
Score: 132
Queries matched: 3
gi|914833
Mass: 42442
Score: 132
Queries matched: 3
keratin type II
Mass: 60008
Score: 132
Queries matched: 3
gi|5031839
keratin 6A [Homo sapiens]
gi|30584049
Mass: 60121
Score: 132
Queries matched: 3
Homo sapiens keratin 6A [synthetic construct]
gi|46812692
Mass: 60038
Score: 132
Queries matched: 3
Keratin 6A [Homo sapiens]
gi|84040267
Mass: 59960
Score: 132
Queries matched: 3
Keratin 6C [Homo sapiens]
gi|109096775
Mass: 59930
Score: 132
Queries matched: 3
PREDICTED: keratin 6B isoform 7 [Macaca mulatta]
gi|109096777
Mass: 54380
Score: 132
Queries matched: 3
PREDICTED: keratin 6B isoform 5 [Macaca mulatta]
gi|109096781
Mass: 59936
Score: 132
Queries matched: 3
PREDICTED: keratin 6A isoform 18 [Macaca mulatta]
Mass: 56324
Score: 132
Queries matched: 3
gi|109096783
PREDICTED: keratin 6A isoform 6 [Macaca mulatta]
gi|114644568
Mass: 59981
Score: 132
Queries matched: 3
PREDICTED: keratin 6A [Pan troglodytes]
gi|119617035
Mass: 59859
Score: 132
Queries matched: 3
keratin 6E, isoform CRA_b [Homo sapiens]
Mass: 59879
Score: 132
Queries matched: 3
gi|119617036
hCG2039812, isoform CRA_a [Homo sapiens]
gi|155969697
Mass: 59988
Score: 132
Queries matched: 3
keratin 6C [Homo sapiens]
Mass: 59994
Score: 132
Queries matched: 3
gi|158261513
gi|189053798
Mass: 59990
Score: 132
Queries matched: 3
gi|194377194
Mass: 55765
Score: 132
Queries matched: 3
Mass: 55698
Score: 132
Queries matched: 3
gi|194383916
gi|194387918
Mass: 57803
Score: 132
Queries matched: 3
Mass: 58500
Score: 132
Queries matched: 3
gi|194387990
Mass: 58643
Score: 132
Queries matched: 3
gi|194388850
41.
gi|223570
Mass: 22716
Score: 131
Queries matched: 4
emPAI: 0.15
Query
216
270
520
613
Observed
529.8052
565.3110
716.9007
789.4067
Mr(expt)
1057.5958
1128.6074
1431.7868
1576.7988
Mr(calc)
1057.5954
1128.6080
1431.7874
1576.7984
0.45
0
21
7.9
2
-0.49
0
24
3.1
1
-0.42
0
46
0.02
1
0.26
0
40
0.14
1
Peptide
K.MIAIQQNIK.F
K.AIWNVVNWK.E
K.LAGVQGSGWAFIVK.N
K.AIDEQFGSLDELIK.L
gi|6321796
Mass: 25758
Score: 131
Queries matched: 4
Mitochondrial superoxide dismutase, protects cells against oxygen toxicity; phosphorylated; Sod2p [Saccharomyces cerevisiae]
gi|217035334
Mass: 24079
Score: 131
Queries matched: 4
Chain A, The Crystal Structure Of Sod2 From Saccharomyces Cerevisiae
42.
gi|157165151
Mass: 30690
Score: 121
Queries matched: 3
HDOD domain-contain protein [Campylobacter concisus 13826]
emPAI: 0.36
12/9/2009
Query
601
617
843
43.
Observed
778.8805
789.9462
977.9969
Mr(expt)
1555.7464
1577.8778
1953.9792
Mr(calc)
1555.7478
1577.8777
1953.9796
-0.86
0
34
0.45
1
0.09
0
32
0.21
1
-0.17
0
55 0.0036
1
Page 13 of 26
Peptide
R.LHADENSSINDLTK.V
K.ALPPLDDTVIQIQR.L
R.SANSPLYGFSQEITTIAR.A
gi|149716548
Mass: 30312
Score: 116
Queries matched: 3
emPAI: 0.11
Query
316
425
508
44.
Observed
597.3244
646.2998
706.8568
Mr(expt)
1192.6342
1290.5850
1411.6990
Mr(calc)
1192.6340
1290.5840
1411.6983
0.24
0
28
2.2
1
0.79
0
27
1.4
1
0.52
0
61 0.00097
1
gi|157164741
Mass: 20646
Score: 110
Queries matched: 3
adenylate kinase [Campylobacter concisus 13826]
Peptide
K.DLATVYVDAVK.D
K.DGGGASLAEYHSK.A
R.EYVAQFEASALGK.Q
emPAI: 0.16
Query
208
423
475
45.
Observed
524.2691
643.8774
682.7951
Mr(expt)
1046.5236
1285.7402
1363.5756
Mr(calc)
1046.5244
1285.7394
1363.5753
-0.70
0
35
0.31
1
0.65
0
33
0.37
1
0.29
0
42
0.017
1
gi|157164034
Mass: 25720
Score: 106
Queries matched: 2
uridylate kinase [Campylobacter concisus 13826]
Peptide
R.AEVASGSELGK.L
K.NLFLIIGAPGSGK.T
R.GADDNNEVFNNR.M
emPAI: 0.28
Query
615
862
46.
Observed
789.4244
998.5050
Mr(expt)
1576.8342
1994.9954
Mr(calc)
1576.8348
1994.9949
gi|27806963
Mass: 26002
-0.35
0
70 0.00012
1
0.27
0
36
0.27
1
Score: 100
Queries matched: 3
Peptide
K.IINEEEAALYSVVK.K.FSGEALAGENGFGIDTAVLK.F
emPAI: 0.13
Query
140
369
477
47.
Observed
490.2841
623.8246
684.3511
Mr(expt)
978.5536
1245.6346
1366.6876
Mr(calc)
978.5538
1245.6353
1366.6881
-0.19
0
27
2
2
-0.56
1
20
17
1
-0.32
0
53 0.0068
1
Peptide
K.FALPQYLK.T
K.ITVDDKHYQK.A
K.ALNEINQFYQK.F
gi|57167806
Mass: 28165
Score: 98
Queries matched: 3
probable oxidoreductase Cj0807 [Campylobacter coli RM2228]
Query
91
141
582
Observed
452.2664
491.2479
763.9121
Mr(expt)
902.5182
980.4812
1525.8096
Mr(calc)
902.5185
980.4814
1525.8100
-0.32
0
36
0.2
1
-0.20
0
20
9.9
1
-0.25
0
42
0.052
1
Peptide
K.TLVISGGTR.G
R.YAATELGEK.N
R.VNVVSGGPIETDALR.A
gi|86150182
Mass: 28119
Score: 98
Queries matched: 3
oxidoreductase, short chain dehydrogenase/reductase family [Campylobacter jejuni subsp. jejuni CF93-6]
gi|86152197
Mass: 28177
Score: 98
Queries matched: 3
oxidoreductase, short chain dehydrogenase/reductase family [Campylobacter jejuni subsp. jejuni 260.94]
gi|88597491
Mass: 28147
Score: 98
Queries matched: 3
oxidoreductase, short chain dehydrogenase/reductase family [Campylobacter jejuni subsp. jejuni 84-25]
Mass: 28146
Score: 98
Queries matched: 3
gi|148926969
putative oxidoreductase [Campylobacter jejuni subsp. jejuni CG8486]
gi|154173801
Mass: 28163
Score: 98
Queries matched: 3
7-alpha-hydroxysteroid dehydrogenase [Campylobacter curvus 525.92]
gi|157163926
Mass: 28390
Score: 98
Queries matched: 3
7-alpha-hydroxysteroid dehydrogenase [Campylobacter concisus 13826]
gi|157415078
Mass: 28109
Score: 98
Queries matched: 3
7-alpha-hydroxysteroid dehydrogenase [Campylobacter jejuni subsp. jejuni 81116]
gi|223040200
Mass: 28147
Score: 98
Queries matched: 3
7-alpha-hydroxysteroid dehydrogenase [Campylobacter rectus RM3267]
gi|255322252
Mass: 28137
Score: 98
Queries matched: 3
7-alpha-hydroxysteroid dehydrogenase [Campylobacter showae RM3277]
48.
gi|162811
Mass: 21212
kappa-casein precursor
Score: 98
Queries matched: 2
emPAI: 0.16
Query
377
Observed
626.3583
Mr(expt)
1250.7020
Mr(calc)
1250.7023
-0.19
0
28
0.95
1
Peptide
K.YIPIQYVLSR.Y
12/9/2009
857
990.5482
1979.0818
1979.0840
-1.09
0
70
4e-005
1
Page 14 of 26
R.SPAQILQWQVLSNTVPAK.S
gi|226020
Mass: 21140
Score: 98
Queries matched: 2
kappa casein
Mass: 12279
Score: 98
Queries matched: 2
gi|229416
casein para kappaA
gi|300459
Mass: 18912
Score: 98
Queries matched: 2
kappa-casein C [cattle, milk, Peptide Mutant, 169 aa]
gi|4887004
Mass: 17849
Score: 98
Queries matched: 2
kappa-casein precursor [Bos taurus]
gi|4887006
Mass: 17827
Score: 98
Queries matched: 2
gi|7528205
Mass: 17899
Score: 98
Queries matched: 2
kappa-casein [Bos grunniens]
gi|8099320
Mass: 17868
Score: 98
Queries matched: 2
kappa-casein [Bos indicus x Bos taurus]
gi|8099322
Mass: 17880
Score: 98
Queries matched: 2
gi|8099324
Mass: 17852
Score: 98
Queries matched: 2
kappa-casein precursor [Bos taurus x Bos indicus]
gi|22552661
Mass: 18208
Score: 98
Queries matched: 2
kappa-casein long form [Bos grunniens]
gi|22552663
Mass: 17824
Score: 98
Queries matched: 2
kappa-casein short form [Bos grunniens]
gi|27881412
Mass: 21256
Score: 98
Queries matched: 2
casein kappa [Bos taurus]
Mass: 21224
Score: 98
Queries matched: 2
gi|36988716
kappa casein [Bos taurus]
Mass: 17693
Score: 98
Queries matched: 2
gi|40311084
kappa-casein [Bos taurus]
gi|125489161
Mass: 17836
Score: 98
Queries matched: 2
kappa casein [Bos taurus]
gi|156144998
Mass: 17840
Score: 98
Queries matched: 2
kappa-casein [Bos grunniens]
Mass: 19562
Score: 98
Queries matched: 2
gi|166078544
kappa-casein [Bos indicus]
gi|166078546
Mass: 19505
Score: 98
Queries matched: 2
kappa-casein [Bos indicus]
gi|193089919
Mass: 17880
Score: 98
Queries matched: 2
kappa casein [Bos indicus]
49.
gi|157164768
Mass: 27288
Score: 98
Queries matched: 2
emPAI: 0.26
molybdate ABC transporter, periplasmic molybdate-binding protein [Campylobacter concisus 13826]
Query
335
718
50.
Observed
605.8088
856.4470
Mr(expt)
1209.6030
1710.8794
Mr(calc)
1209.6030
1710.8828
0.05
0
69 0.00019
1
-1.97
1
29
1.2
1
gi|154175458
Mass: 18862
Score: 94
Queries matched: 4
Peptide
K.AYDTGFAVAAPK.V
K.IYDEVEKNIVYAQK.I
emPAI: 0.18
Query
182
342
363
569
Observed
510.2690
613.7896
621.7871
755.3728
Mr(expt)
1018.5234
1225.5646
1241.5596
1508.7310
Mr(calc)
1018.5236
1225.5649
1241.5598
1508.7300
-0.18
0
28
3.3
3
-0.19
0
(36)
0.2
1
-0.12
0
42
0.042
1
0.72
0
24
5.1
1
Peptide
K.FQWTGGPVK.K.MDTGVGNYELK.F
K.MDTGVGNYELK.F + Oxidation (M)
K.NGFGEGEWIPYLK.I
gi|157165691
Mass: 18808
Score: 94
Queries matched: 4
51.
gi|494583
Mass: 15714
Score: 93
Queries matched: 3
emPAI: 0.22
Query
45
157
229
Observed
414.2711
499.8032
541.7649
Mr(expt)
826.5276
997.5918
1081.5152
Mr(calc)
826.5276
997.5920
1081.5152
0.02
0
25
1.8
1
-0.19
0
46
0.017
1
0.00
0
22
7
1
Peptide
-.VQAVAVLK.G
K.LIGPTSVVGR.S
K.THGAPTDEVR.H
gi|6322564
Mass: 15845
Score: 93
Queries matched: 3
gi|6730103
Mass: 15680
Score: 93
Queries matched: 3
12/9/2009
Page 15 of 26
gi|15826571
Mass: 15724
Score: 93
Queries matched: 3
gi|27573538
Mass: 15944
Score: 93
Queries matched: 3
gi|27573539
Mass: 15836
Score: 93
Queries matched: 3
gi|27573540
Mass: 15811
Score: 93
Queries matched: 3
52.
gi|2232299
Mass: 47885
Score: 93
Queries matched: 8
emPAI: 0.07
Query
237
252
397
398
764
765
775
777
Observed
547.2814
554.2891
633.8091
633.8096
595.3299
892.4925
600.6619
900.4901
Mr(expt)
1092.5482
1106.5636
1265.6036
1265.6046
1782.9679
1782.9704
1798.9639
1798.9656
Mr(calc)
1092.5485
1106.5641
1265.6040
1265.6040
1782.9702
1782.9702
1798.9651
1798.9651
-0.24
0
33
0.63
1
-0.45
0
(22)
8.8
1
-0.30
0
23
7
1
0.49
0
(20)
11
1
-1.31
0
(25)
2.4
1
0.13
0
37
0.12
1
-0.70
0
(25)
2.7
1
0.29
0
(25)
2.7
1
Peptide
K.ALETHTYFER.H
K.ALETHTYFER.H
K.DVAMKPPSVYLLPPTR.E + Oxidation (M)
gi|3834667
Mass: 48183
Score: 93
Queries matched: 8
gi|24496448
Mass: 65621
Score: 93
Queries matched: 8
gi|33413902
Mass: 49106
Score: 93
Queries matched: 8
gi|34538498
Mass: 49909
Score: 93
Queries matched: 8
53.
gi|157165617
Mass: 27356
Score: 92
Queries matched: 1
emPAI: 0.12
NAD-dependent epimerase/dehydratase family protein [Campylobacter concisus 13826]
Query
661
54.
Observed
828.4237
Mr(expt)
1654.8328
Mr(calc)
1654.8315
ppm Miss Score
Expect Rank
0.82
0
92 8.2e-007
1
gi|126742337
Mass: 15355
Score: 88
hemoglobin alpha chain [Ochotona curzoniae]
Peptide
K.TAFVTGATSGFGEAIAR.R
Queries matched: 5
Query
233
572
573
574
790
55.
Observed
544.3166
758.3622
505.9110
758.3629
611.9684
Mr(expt)
1086.6186
1514.7098
1514.7112
1514.7112
1832.8834
Mr(calc)
1086.6186
1514.7113
1514.7113
1514.7113
1832.8846
0.07
1
22
7.8
1
-0.99
0
(28)
1.6
1
-0.12
0
(39)
0.09
2
-0.07
0
44
0.033
1
-0.68
0
22
7.4
1
gi|158604950
Mass: 13498
Score: 88
Queries matched: 2
Peptide
K.LRVDPVNFK.L
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
K.TYFPHFDVTHGSAQVK.A
emPAI: 0.57
Query
449
621
56.
Observed
660.8350
794.4047
Mr(expt)
1319.6554
1586.7948
Mr(calc)
1319.6568
1586.7940
gi|538275
Mass: 22697
Score: 82
-1.06
0
39
0.2
1
0.52
0
49
0.016
1
Queries matched: 2
Peptide
R.STAAAAAIDSSDLK.I
R.TPSEWQETGIIAGAK.T
emPAI: 0.15
Query
106
265
Observed
465.7662
561.3131
Mr(expt)
929.5178
1120.6116
Mr(calc)
929.5182
1120.6128
-0.37
0
25
5.7
5
-1.05
0
57 0.0015
1
Peptide
K.LTPETLTR.W
K.DTQSIIFLGK.V
gi|1245695
Mass: 28553
Score: 82
Queries matched: 2
Mass: 46175
Score: 82
Queries matched: 2
gi|31340900
gi|126165236
Mass: 46368
Score: 82
Queries matched: 2
Mass: 46208
Score: 82
Queries matched: 2
gi|160332365
gi|226373737
Mass: 46208
Score: 82
Queries matched: 2
12/9/2009
Page 16 of 26
57.
gi|157165298
Mass: 25904
Score: 82
Queries matched: 3
cytochrome c oxidase, diheme subunit, membrane-bound [Campylobacter concisus 13826]
Query
100
361
922
58.
Observed
458.2666
618.3476
734.4042
Mr(expt)
914.5186
1234.6806
2200.1908
Mr(calc)
914.5185
1234.6809
2200.1926
0.17
1
26
6.7
1
-0.19
1
28
1
1
-0.82
0
27
0.95
1
Peptide
K.GIEKLEAR.N
K.VYDKIDELLK.S
K.IVQDAPQMVSPITLFVLSAR.A + Oxidation (M)
gi|157164484
Mass: 26377
Score: 74
Queries matched: 2
3-ketoacyl-(acyl-carrier-protein) reductase [Campylobacter concisus 13826]
Query
107
330
Observed
465.7722
603.8275
Mr(expt)
929.5298
1205.6404
Mr(calc)
929.5294
1205.6404
0.46
0
43
0.095
1
0.03
1
31
1.1
1
Peptide
K.NVLITGASR.G
K.TYSDNIPLKR.F
gi|255322801
Mass: 26267
Score: 74
Queries matched: 2
3-oxoacyl-[acyl-carrier-protein] reductase [Campylobacter showae RM3277]
59.
gi|3928489
Mass: 222566
Score: 72
Queries matched: 4
Query
50
58
63
172
Observed
417.7286
421.7708
425.7262
505.8159
Mr(expt)
833.4426
841.5270
849.4378
1009.6172
Mr(calc)
833.4429
841.5273
849.4378
1009.6172
-0.32
0
(26)
5.9
1
-0.30
0
26
1.6
1
0.01
0
26
5.2
1
0.04
0
20
1.7
4
Peptide
R.LQSMTVR.Q
K.LTIPAVTK.A
R.LQSMTVR.Q + Oxidation (M)
R.VTGIPTPVVK.F
gi|17066106
Mass: 631230
Score: 72
Queries matched: 4
gi|33871368
Mass: 32739
Score: 72
Queries matched: 4
Mass: 60186
Score: 72
Queries matched: 4
gi|37573987
Mass: 69051
Score: 72
Queries matched: 4
gi|47124504
gi|85543964
Mass: 21554
Score: 72
Queries matched: 4
gi|110349721
Mass: 631174
Score: 72
Queries matched: 4
gi|110590764
Mass: 20706
Score: 72
Queries matched: 4
gi|119631421
Mass: 631293
Score: 72
Queries matched: 4
gi|194043971
Mass: 147632
Score: 72
Queries matched: 4
Mass: 3708275 Score: 72
Queries matched: 4
gi|194222358
gi|17066104
Mass: 2991182 Score: 71
Queries matched: 4
gi|17066105
Mass: 3813840 Score: 71
Queries matched: 4
gi|108861911
Mass: 3813810 Score: 71
Queries matched: 4
gi|110349713
Mass: 3004958 Score: 71
Queries matched: 4
Mass: 2991151 Score: 71
Queries matched: 4
gi|110349715
gi|110349717
Mass: 3012156 Score: 71
Queries matched: 4
gi|110349719
Mass: 3711359 Score: 71
Queries matched: 4
gi|119631418
Mass: 3878844 Score: 71
Queries matched: 4
gi|119631420
Mass: 2991188 Score: 71
Queries matched: 4
gi|119631423
Mass: 3004995 Score: 71
Queries matched: 4
gi|119631424
Mass: 3012223 Score: 71
Queries matched: 4
gi|225131084
Mass: 3711397 Score: 71
Queries matched: 4
12/9/2009
60.
gi|157165622
Mass: 24092
Score: 70
Queries matched: 2
integrase/recombinase [Campylobacter concisus 13826]
Page 17 of 26
emPAI: 0.14
Query
284
413
61.
Observed
577.7879
639.3219
Mr(expt)
1153.5612
1276.6292
Mr(calc)
1153.5615
1276.6299
-0.23
1
21
6.3
1
-0.50
1
49
0.016
1
Peptide
K.TKGDYGEVASK.V
K.ADYEKELANPK.V
gi|157164291
Mass: 25651
Score: 69
Queries matched: 2
response regulator receiver domain-containing protein [Campylobacter concisus 13826]
Query
62
67
62.
Observed
425.2445
426.7083
Mr(expt)
848.4744
851.4020
Mr(calc)
848.4756
851.4025
-1.36
0
38
0.24
1
-0.51
0
31
0.96
1
Peptide
R.FGGTNIIK.I
R.AGADDYIK.K
gi|157165615
Mass: 27439
Score: 66
Queries matched: 2
adenylosuccinate synthetase (imp--aspartate ligase; adss; ampsase) [Campylobacter concisus 13826]
Query
161
253
63.
Observed
500.7928
554.3056
Mr(expt)
999.5710
1106.5966
Mr(calc)
999.5713
1106.5971
-0.21
1
25
5.1 10
-0.44
0
41
0.075
1
Peptide
K.LKENLAQGK.A
K.SNLQYEIIK.S
gi|157165413
Mass: 23563
Score: 66
Queries matched: 2
Query
346
347
64.
Observed
615.3062
615.8560
Mr(expt)
1228.5978
1229.6974
Mr(calc)
1228.5976
1229.6979
0.23
0
32
0.52
1
-0.39
1
34
0.29
1
gi|157164911
Mass: 31228
Score: 66
Queries matched: 1
putative periplasmic protein [Campylobacter concisus 13826]
Peptide
K.EGLGSVEFYTK.E
K.KLEIVQTSNAK.T
emPAI: 0.11
Query
492
65.
Observed
693.8830
Mr(expt)
1385.7514
Mr(calc)
1385.7514
0.02
0
66 0.00022
1
Peptide
K.TTEQDALNLLIR.D
gi|146296629
Mass: 31331
Score: 64
Queries matched: 3
NLPA lipoprotein [Caldicellulosiruptor saccharolyticus DSM 8903]
Query
74
145
146
66.
Observed
429.2397
493.2869
493.2873
Mr(expt)
856.4648
984.5592
984.5600
Mr(calc)
856.4654
984.5604
984.5604
-0.65
0
28
1.5
1
-1.13
1
36
0.26
1
-0.32
1
(35)
0.37
1
gi|157164695
Mass: 21029
Score: 63
Queries matched: 3
elongation factor P [Campylobacter concisus 13826]
Peptide
K.ALNSPEVK.K
K.ALNSPEVKK.F
K.ALNSPEVKK.F
emPAI: 0.16
Query
240
259
694
67.
Observed
550.2762
558.2738
844.4360
Mr(expt)
1098.5378
1114.5330
1686.8574
Mr(calc)
1098.5379
1114.5328
1686.8577
-0.06
1
33
0.58
1
0.19
1
(22)
4.5
1
-0.16
1
30
1.1
1
Peptide
M.ASYSMGDLKK.G
M.ASYSMGDLKK.G + Oxidation (M)
K.IVETPPNFKGDTQGGK.K
gi|149721282
Mass: 28980
Score: 59
Queries matched: 2
emPAI: 0.11
PREDICTED: similar to Carbonic anhydrase 1 (Carbonic anhydrase I) (Carbonate dehydratase I) (CA-I) [Equus caballus]
Query
165
646
68.
Observed
502.2744
811.4130
Mr(expt)
1002.5342
1620.8114
Mr(calc)
1002.5346
1620.8107
-0.31
0
26
3.7
1
0.45
0
34
0.46
1
gi|157164497
Mass: 29815
Score: 59
Queries matched: 2
Peptide
R.SLLSNVEGGK.A
K.ENISISSEQLAQFR.S
emPAI: 0.11
Query
30
540
Observed
403.2444
736.3911
Mr(expt)
804.4742
1470.7676
Mr(calc)
804.4745
1470.7679
-0.35
0
24
2
2
-0.14
0
35
0.34
1
Peptide
K.VFLEAVK.Y
12/9/2009
69.
gi|27806789
Mass: 15717
Score: 57
Queries matched: 3
Page 18 of 26
emPAI: 0.22
Query
89
506
507
Observed
450.2509
704.8207
704.8221
Mr(expt)
898.4872
1407.6268
1407.6296
Mr(calc)
898.4872
1407.6306
1407.6306
0.02
0
26
2
1
-2.70
0
31
0.54
1
-0.71
0
(30)
0.62
1
Peptide
R.GSPAANVGVK.V
K.AADETWEPFASGK.T
K.AADETWEPFASGK.T
gi|57526651
Mass: 15761
Score: 57
Queries matched: 3
70.
gi|154173631
Mass: 19261
Score: 54
Queries matched: 1
emPAI: 0.17
Query
422
Observed
643.8510
Mr(expt)
1285.6874
Mr(calc)
1285.6878
-0.25
0
54 0.0047
1
Peptide
K.SNLLDVLNDGVK.-
gi|157165227
Mass: 19284
Score: 54
Queries matched: 1
71.
gi|157165326
Mass: 18857
Score: 53
Queries matched: 2
Query
301
479
72.
Observed
584.7726
685.8586
Mr(expt)
1167.5306
1369.7026
gi|27806907
Mass: 51081
clusterin [Bos taurus]
Mr(calc)
1167.5309
1369.7030
-0.20
0
20
8.7
1
-0.28
0
33
0.63
1
Score: 51
Queries matched: 1
Peptide
K.AGGHWDPAETK.M
K.TNYGVAFFPNLK.G
emPAI: 0.06
Query
581
Observed
763.8959
Mr(expt)
1525.7772
Mr(calc)
1525.7776
-0.25
0
51
0.007
1
Peptide
R.LYDQLLQSYQQK.M
gi|47522770
Mass: 51741
Score: 51
Queries matched: 1
clusterin [Sus scrofa]
gi|90569238
Mass: 12512
Score: 51
Queries matched: 1
complement cytolysis inhibitor [Sus scrofa]
73.
gi|3914346
Mass: 82383
Score: 49
Queries matched: 2
Query
125
315
Observed
478.7740
596.8196
Mr(expt)
955.5334
1191.6246
Mr(calc)
955.5338
1191.6248
-0.41
0
23
3.3
1
-0.09
1
26
3.5
1
Peptide
K.VVQGEPSLK.V
R.GLIKEQYEGR.L
Query
261
573
513
118
144
541
734
450
40
262
13
821
135
635
257
79
106
49
704
Observed
559.3343
505.9110
710.8634
474.7628
492.3035
736.4034
865.4146
663.3198
407.7482
560.8289
373.2137
636.2845
487.2873
802.9416
557.7616
435.7738
465.7662
416.7478
848.9780
Mr(expt)
1116.6540
1514.7112
1419.7122
947.5110
982.5924
1470.7922
1728.8146
1324.6250
813.4818
1119.6432
744.4128
1905.8317
972.5600
1603.8686
1113.5086
869.5330
929.5178
831.4810
1695.9414
Mr(calc)
1116.6576
1514.7113
1419.7146
947.5110
982.5923
1470.7930
1728.8141
1324.6259
813.4821
1119.6441
744.4130
1905.8315
972.5604
1603.8682
1113.5091
869.5334
929.5182
831.4814
1695.9407
ppm Miss Score
-3.20
1
52
-0.12
0
49
-1.69
0
49
0.08
0
49
0.12
0
49
-0.51
0
46
0.32
0
45
-0.63
0
45
-0.29
1
42
-0.74
0
40
-0.20
0
40
0.08
0
40
-0.32
1
39
0.28
0
38
-0.39
0
38
-0.46
0
38
-0.37
0
38
-0.42
0
37
0.46
0
37
Expect Rank
0.0041
1
0.01
1
0.016
1
0.025
1
0.0056
1
0.03
1
0.033
1
0.031
1
0.14
1
0.068
1
0.48
1
0.055
1
0.16
1
0.093
1
0.11
1
0.21
1
0.29
1
0.2
1
0.088
1
Peptide
MIEILLKNK + Oxidation (M)
VGAHAGDYGAEALER
YVNWIQQTIAAN
MSELLISR
ALGILNLNR
ISPDTQGVLVTDVK
STDAYELSVANR
RDVAVVR
VAIVVPFGYR
DAAVTLR
ADYEAHNVYACEVSHK + Acrylamide (C)
NIKADALTK
VSGVVSANYLAQLQR
GEFSQEVYR
VVSLSIPR
ITEITPTR
LSGSSIIR
ISVELIAPVALEEGTR
12/9/2009
Page 19 of 26
129
954
2
314
76
182
604
730
1
274
603
482.2788
804.7469
353.7159
596.3180
432.7448
510.2690
783.8734
862.4494
353.7156
572.7690
783.8732
962.5430
2411.2189
705.4172
1190.6214
863.4750
1018.5234
1565.7322
1722.8842
705.4166
1143.5234
1565.7318
962.5437
2411.2179
705.4174
1190.6217
863.4752
1018.5229
1565.7283
1722.8828
705.4174
1143.5230
1565.7283
-0.63
0.40
-0.16
-0.18
-0.23
0.49
2.52
0.82
-1.01
0.40
2.26
0
1
0
0
0
1
1
0
0
1
1
36
36
36
34
34
34
33
33
33
32
32
0.23
0.25
0.29
0.33
0.6
0.83
0.43
0.5
0.56
0.43
0.75
1
1
1
1
1
1
1
1
1
1
1
EILSNLFK
ALGLEEDIAKEQLEAANEEIAR
YVVGIR
AGSFEIIK
KMKPGDATR + Oxidation (M)
VTNFEDPYLGLSAGIK
YVVGIR
YSSTKQECK + Acrylamide (C)
119
75
754
890
161
234
140
216
122
16
70
212
712
59
474.7629
429.7681
881.9172
1021.9880
500.7928
545.2985
490.2841
529.8052
475.7505
381.7289
428.7661
529.2726
850.9160
422.7478
947.5112
857.5216
1761.8198
2041.9614
999.5710
1088.5824
978.5536
1057.5958
949.4864
761.4432
855.5176
1056.5306
1699.8174
843.4810
947.5110
857.5222
1761.8210
2041.9633
999.5713
1088.5826
978.5538
1057.5954
949.4869
761.4436
855.5178
1056.5314
1699.8239
843.4814
0.29
-0.65
-0.64
-0.89
-0.23
-0.14
-0.20
0.44
-0.44
-0.43
-0.19
-0.71
-3.81
-0.44
0
0
1
0
1
0
0
0
0
0
0
0
1
1
31
31
31
30
30
30
30
29
29
29
29
28
28
28
1.4
0.73
0.76
0.89
1.6
1.4
1.2
1.1
2.4
1.2
1.7
0.96
1.6
5.5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
MSELLISR
ADVLILSK
SSDKQFYVFNDVDAK
VLYSSEAFPTNFGDPEAAK
QLEAVGAKGK
STGVTAIADVR
FAISVPFAK
MIALALGGGGAK
ADAIIEYR
IFQLGGK
LATVVTPR
LFNCERYITDELK + Carbamidomethyl (C)
LADVLRTG
138
23
179
747
258
345
368
160
42
502
14
193
511
491
48
18
448
463
136
30
28
519
47
424
84
509
786
215
488.2790
395.2391
508.7722
879.4565
557.8452
614.8428
623.2896
500.3135
410.7241
703.4050
376.2208
514.7898
708.8882
693.3512
416.2501
383.2289
660.3189
676.8859
487.7792
403.2444
400.7655
715.8781
415.7293
644.8301
439.7527
708.3560
914.4414
529.2805
974.5434
788.4636
1015.5298
1756.8984
1113.6758
1227.6710
1244.5646
998.6124
819.4336
1404.7954
750.4270
1027.5650
1415.7618
1384.6878
830.4856
764.4432
1318.6232
1351.7572
973.5438
804.4742
799.5164
1429.7416
829.4440
1287.6456
877.4908
1414.6974
1826.8682
1056.5464
974.5470
788.4644
1015.5298
1756.8995
1113.6757
1227.6719
1244.5646
998.6124
819.4347
1404.7950
750.4276
1027.5662
1415.7667
1384.6874
830.4862
764.4432
1318.6187
1351.7612
973.5444
804.4745
799.5167
1429.7412
829.4446
1287.6419
877.4909
1414.6980
1826.8686
1056.5465
-3.66
-0.92
0.05
-0.62
0.10
-0.71
0.02
0.02
-1.24
0.35
-0.67
-1.13
-3.43
0.30
-0.62
0.03
3.46
-2.95
-0.54
-0.35
-0.33
0.30
-0.71
2.93
-0.05
-0.40
-0.21
-0.03
1
0
0
0
1
0
0
0
0
1
1
1
1
0
0
1
0
0
1
1
1
1
0
1
0
0
0
1
28
27
27
27
26
26
26
26
26
26
26
26
26
26
25
25
25
25
25
25
24
24
24
24
24
23
23
23
3.2
1.2
3.4
2.2
0.94
2.3
1.4
2
3
1.3
5
3
2.4
2.8
5.5
3.1
3.1
1.6
4.4
1.8
4.3
3.9
3.6
5.5
5.2
4.7
5.7
5.4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
ECIEIIKK
IVTVETK
ADLSNELVR
LYPIANGDNQSPIDIK
LDIGTIINKK
LAPMVGLSCPLK
HHANEAEAGPGR
LTSTIPVLR
MLCAVGVK
QRLDNLLQHLR
KAIEYK
AKVVGGDAPSK
GMGRLVLSAVQNR + Oxidation (M)
SFLGEAYDGLSVK
SLVVANTK
KIFETK
TECELLNNEVR
GVALTWPVSGLPR
KVEEIAASK
VFKALDL
KIIIDAK
EAADLAKESQGIAK
GLGVYHGK
ALADKAGDSGVER
IINDYIK
TFIDGVVESYASK
GEADALNLDGGYIYTAGK
WVGRTPADR
619
156
679
458
223
433
25
172
462
553
200
248
95
178
792.9188
499.3110
834.9256
674.3358
537.2955
651.8398
398.2025
505.8159
676.8202
745.3860
516.7881
551.3345
453.7503
508.7487
1583.8230
996.6074
1667.8366
1346.6570
1072.5764
1301.6650
794.3904
1009.6172
1351.6258
1488.7574
1031.5616
1100.6544
905.4860
1015.4828
1583.8235
996.6080
1667.8366
1346.6612
1072.5764
1301.6616
794.3922
1009.6172
1351.6305
1488.7606
1031.5651
1100.6553
905.4827
1015.4822
-0.31
-0.56
0.02
-3.10
0.04
2.68
-2.26
0.09
-3.44
-2.14
-3.39
-0.79
3.72
0.66
0
1
0
1
1
0
1
0
0
1
1
1
0
0
23
23
23
23
23
22
22
22
22
22
22
22
22
22
4.7
1.5
7.2
6.2
6.6
7.6
9.6
1.1
6.2
8.3
8
2.4
9.6
5.9
1
1
1
1
1
1
1
1
1
1
1
1
1
1
YGLLTAPSDFPIYK
GQSLVIPKR
IIPSAEDPSQDIVER
DALEEAGLKK
VYLDVAHTGEAK
RYSLEQ
AEGLVPALLK
MIFCSVGFGFTK + Oxidation (M)
DCTGVTVRITPEK + Acrylamide (C)
FEPDVGKIK
ASAQALALTKK
MSVCVVLR
TPELEENGK
96
210
209
137
467
364
551
861
222
453.7613
525.2906
525.2904
488.2789
679.3627
621.8187
743.8967
661.6894
358.5325
905.5080
1048.5666
1048.5662
974.5432
1356.7108
1241.6228
1485.7788
1982.0464
1072.5757
905.5044
1048.5665
1048.5699
974.5397
1356.7071
1241.6252
1485.7762
1982.0506
1072.5764
3.98
0.11
-3.48
3.68
2.73
-1.89
1.79
-2.16
-0.71
0
1
1
0
0
0
1
0
0
21
21
21
21
21
21
21
21
21
8.5
11
9.7
14
11
7.6
7.1
5.4
9.5
1
1
1
1
1
1
1
1
1
YCIVAIPK
FRENLGSVK
ITLKASTCR + Carbamidomethyl (C)
EGLSVTTIR
QMTDGPGVLQAIK
DGAVVVVESPDR
LPSMKPEWQSKR
VSLTNDIGQLLSCIHEIK
IEDATVGLAGK
12/9/2009
Page 20 of 26
735
184
211
323
365
69
841
341
600
416
725
578.6317
510.7717
526.2637
601.3483
622.3477
428.7661
973.4887
610.7987
777.8680
641.2839
573.9708
1732.8733
1019.5288
1050.5128
1200.6820
1242.6808
855.5176
1944.9628
1219.5828
1553.7214
1280.5532
1718.8906
1732.8706
1019.5321
1050.5128
1200.6866
1242.6819
855.5178
1944.9615
1219.5867
1553.7251
1280.5529
1718.8913
1.56
-3.19
0.06
-3.83
-0.88
-0.15
0.71
-3.12
-2.38
0.23
-0.40
1
1
0
1
1
0
1
1
1
0
0
21
21
21
21
21
20
20
20
20
20
20
11
14
13
6.9
7.5
12
13
14
9.1
5.2
13
1
1
1
1
1
1
1
1
1
1
1
EGVPMATPVFDGIKEK + Oxidation (M)
SIEMKVEGK
IKFVPENVQK
KVDSLQSLPEK
IAISLPSR
FAQETAAPMQKQEPIEK
GAAQTKMEADAK
MAEKMLVMTSNDGK
MPEDCVPTFR + Acrylamide (C); Oxidation (M)
IAYTSNEPMPTAALLK
103
530
117
241
691
313
192
937
557
636
278
461
232
53
460.2219
483.9374
472.7273
550.3090
562.9720
590.8524
513.8009
767.0633
746.8790
803.3971
573.8113
676.3586
363.2133
421.2299
918.4292
1448.7904
943.4400
1098.6034
1685.8942
1179.6902
1025.5872
2298.1681
1491.7434
1604.7796
1145.6080
1350.7026
1086.6181
840.4452
918.4294
1448.7888
943.4399
1098.6033
1685.8961
1179.6903
1025.5869
2298.1678
1491.7417
1604.7794
1145.6081
1350.7031
1086.6186
840.4454
-0.18
1.07
0.13
0.12
-1.15
-0.08
0.31
0.12
1.20
0.12
-0.01
-0.32
-0.46
-0.15
0
1
1
1
1
0
1
0
1
0
0
1
1
0
20
20
20
20
19
19
19
19
19
19
19
19
19
19
7.5
7.7
6.1
11
10
2.6
8.5
11
14
14
23
15
17
7.2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
TDIENAEK
VVAGVANALAHKYH
YDEFKSR
ETPVNKGGIGK
AAAELAPGPEVHARAAR
VLIVVSPYYK
AGIAKLDGGPK
EDDSVETLRSTIK
ADVVFNQVSINEDR
SVVAEPLFER
LKTQDLDDYLK
LRVDPVNFK
HTVEVTR
465
66
609
814
797
396
164
555
516
611
120
421
813
481
550
788
419
85
159
799
22
457
183
272
847
438
846
418
679.3588
426.2288
524.5988
632.6478
616.6694
422.8748
501.7946
497.8999
713.8457
788.3859
475.7138
642.8607
948.4676
460.5645
496.2666
610.3013
642.3410
442.2496
500.2484
924.5018
395.2271
673.8875
510.7714
378.8802
654.0328
436.5695
980.5448
641.8484
1356.7030
850.4430
1570.7746
1894.9216
1846.9864
1265.6026
1001.5746
1490.6779
1425.6768
1574.7572
949.4130
1283.7068
1894.9206
1378.6717
1485.7780
1827.8821
1282.6674
882.4846
998.4822
1846.9890
788.4396
1345.7604
1019.5282
1133.6188
1959.0766
1306.6867
1959.0750
1281.6822
1356.7038
850.4436
1570.7739
1894.9214
1846.9862
1265.6074
1001.5757
1490.6758
1425.6776
1574.7610
949.4109
1283.7020
1894.9214
1378.6762
1485.7787
1827.8825
1282.6703
882.4858
998.4821
1846.9863
788.4392
1345.7619
1019.5321
1133.6227
1959.0789
1306.6881
1959.0789
1281.6789
-0.54
-0.65
0.40
0.11
0.08
-3.82
-1.06
1.36
-0.53
-2.37
2.21
3.80
-0.37
-3.30
-0.47
-0.25
-2.26
-1.28
0.12
1.51
0.56
-1.06
-3.80
-3.43
-1.19
-1.11
-1.97
2.58
1
0
0
1
1
1
0
1
0
0
0
1
1
0
0
0
1
1
0
0
0
1
0
1
1
1
1
1
19
19
19
19
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
16
15
15
17
9.2
19
12
10
16
18
6.3
14
20
24
19
17
16
6.1
15
11
30
10
33
17
7.8
20
8.5
15
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
GVTFTDRGTYIK
EIIADYK
SVHSATSNWAAIAEK
HFGKDFTPELQASYQK
IAYTSNEPMPTAALLKK
SPKELGDFMSR
LVVSTQTALA
DPFMGLHREMSR + Oxidation (M)
DFTPELQASYQK
QVMAENVRLPK
HFGKDFTPELQASYQK
QQMTSTENTVLK
LASLAENSLNAVER
LGENCDADILFVHSPK + Acrylamide (C)
NCSFKITTTIR
IIGHCKR + Carbamidomethyl (C)
FHIDNPEK
LLPGSAYGEQSCVGLLLK
IVSSDIR
WHGLSQHIKIK
MNSVTELVK
AVTSSLRICK + Carbamidomethyl (C)
QLVEIKEVNLDPNPPVR
GSFLIDKDGTVR
QLVEIKEVNLDPNPPVR
QGKAHQGTLTNK
292
870
154
204
218
429
440
430
512
723
41
338
585
247
580.8112
1005.9930
496.7980
523.2584
532.7614
433.2468
655.3228
649.3668
709.3699
572.9557
409.7240
608.8502
510.6154
551.3344
1159.6078
2009.9714
991.5814
1044.5022
1063.5082
1296.7186
1308.6310
1296.7190
1416.7252
1715.8453
817.4334
1215.6858
1528.8244
1100.6542
1159.6084
2009.9694
991.5815
1044.5022
1063.5087
1296.7224
1308.6350
1296.7190
1416.7282
1715.8438
817.4334
1215.6823
1528.8222
1100.6554
-0.51
1.00
-0.00
0.02
-0.41
-2.94
-3.03
0.03
-2.10
0.85
0.03
2.91
1.39
-1.01
0
0
1
0
0
0
0
0
1
0
0
0
1
1
18
18
17
17
17
17
17
17
17
17
17
17
17
17
17
19
6.9
18
22
14
20
14
26
24
12
23
24
7.7
1
1
1
1
1
1
1
1
1
1
1
1
1
1
QTEISIDNIK
YNVGNPGSGNEVSTLEVFK
KTLPAPTHK
CGANQITGPK + Carbamidomethyl (C)
YPQSIDWR
IVQAGGQCAPILK
FINGDKPEDFK
IVEYQHVKPGK
SIKLNETPAMGEK
QISNLQQSISDAEQR
VSNVGFVP
IAVVLDTGSVSR
AGNADLPLHGGRVPR
LNGTGVVSVKK
33
173
110
933
24
392
698
761
736
403.7112
505.8159
467.7715
761.7320
397.7058
633.3223
563.3177
883.8952
578.6320
805.4078
1009.6172
933.5284
2282.1742
793.3970
1264.6300
1686.9313
1765.7758
1732.8742
805.4082
1009.6172
933.5284
2282.1729
793.3970
1264.6274
1686.9305
1765.7802
1732.8744
-0.50
0.04
0.09
0.57
0.04
2.07
0.47
-2.45
-0.13
1
0
0
0
1
0
1
1
1
17
17
17
17
17
17
17
17
16
19
3.8
36
21
31
33
13
14
24
1
1
1
1
1
1
1
1
1
FARQGEV
VTGIPTPVVK
KPTVNFTK
IITHPNFNGNTLDNDIMLIK
FAKSGGEV
IPFQFTCNPK + Acrylamide (C)
TGVDIKWTAVGTGAALK
WECVSGSGTKNSNQNR
ADVVFNQVSINEDRK
12/9/2009
Page 21 of 26
318
751
826
245
72
367
185
219
271
471
478
598.8659
587.6479
641.3751
550.3270
429.2106
623.2896
511.2330
532.7958
565.7695
681.9030
685.8583
1195.7172
1759.9219
1921.1035
1098.6394
856.4066
1244.5646
1020.4514
1063.5770
1129.5244
1361.7914
1369.7020
1195.7176
1759.9178
1921.1037
1098.6397
856.4079
1244.5633
1020.4546
1063.5774
1129.5251
1361.7918
1369.7030
-0.30
2.29
-0.11
-0.22
-1.46
1.09
-3.09
-0.35
-0.60
-0.28
-0.72
0
0
1
1
0
0
0
0
0
1
0
16
16
16
16
16
16
16
16
16
16
16
4.6
20
3.7
24
16
14
21
24
13
5.8
27
1
1
1
1
1
1
1
1
1
1
1
QGLLPVLESLK
SSLGSDMEFPVPILLR
IVEIPGEVPPGKYVVVAR
LLDDVRLQK
QAYSNFK
SAAQDPDSIEGR
EGLAAIHTPR
DQPTIEQGDK
VLELASNIKTFK
TNYGVAFFPNLK
275
589
758
820
480
529
778
285
647
384
571
92
791
565
382.5555
766.8920
589.2842
636.0147
689.3103
483.9371
903.4839
577.8358
543.9783
630.2973
756.8804
452.2684
611.9686
502.5717
1144.6447
1531.7694
1764.8308
1905.0223
1376.6060
1448.7895
1804.9532
1153.6570
1628.9131
1258.5800
1511.7462
902.5222
1832.8840
1504.6933
1144.6452
1531.7705
1764.8287
1905.0207
1376.6055
1448.7888
1804.9604
1153.6567
1628.9137
1258.5764
1511.7468
902.5226
1832.8846
1504.6915
-0.44
-0.67
1.17
0.81
0.38
0.45
-3.98
0.27
-0.40
2.89
-0.33
-0.34
-0.33
1.20
0
0
0
0
1
1
1
1
1
1
1
0
0
0
16
16
16
16
16
16
16
16
16
15
15
15
15
15
23
32
27
16
14
20
28
18
13
21
39
10
36
26
1
1
1
1
1
1
1
1
1
1
1
1
1
1
LVSASATAQAVK
TVGVHLEPMGVYSK + Oxidation (M)
ECHSLIILSSSNCFR + Carbamidomethyl (C)
IAVVNGDGIGPEVIPEAQK
KEDEAESDAGAGAK
VVAGVANALAHKYH
AVDQMSKSTVIAVESIK
SRLPGIVAEGR
NVSKELIPIFVNEK
CAEYYKQGAR + Acrylamide (C)
DSGLTSDLSSFAGKK
SVNPFVIK
TYFPHFNLSHGSAEVK
DALYAACLHAACR + Acrylamide (C); Carbamidomethyl (C)
667
267
564
602
517
329
669
532
658
699
616
951
105
940
942
670
975
51
776
576
307
756
524
123
514
713
956
293
829.4677
563.7855
752.8669
779.9202
714.3679
603.3374
830.9002
486.9001
548.9644
844.4741
526.9664
804.6757
465.7195
770.3994
797.7455
554.2958
968.7982
418.7473
600.6623
506.2654
587.7737
588.6453
722.3256
476.2606
710.9059
567.6132
808.0445
581.7903
1656.9208
1125.5564
1503.7192
1557.8258
1426.7212
1204.6602
1659.7858
1457.6785
1643.8714
1686.9336
1577.8774
2411.0053
929.4244
2308.1764
2390.2147
1659.8656
2903.3728
835.4800
1798.9651
1515.7744
1173.5328
1762.9141
1442.6366
950.5066
1419.7972
1699.8178
2421.1117
1161.5660
1656.9199
1125.5567
1503.7214
1557.8263
1426.7238
1204.6604
1659.7861
1457.6787
1643.8777
1686.9345
1577.8712
2411.0084
929.4243
2308.1739
2390.2151
1659.8654
2903.3753
835.4803
1798.9676
1515.7756
1173.5336
1762.9076
1442.6347
950.5073
1419.7973
1699.8206
2421.1118
1161.5675
0.59
-0.23
-1.41
-0.30
-1.81
-0.16
-0.14
-0.13
-3.86
-0.48
3.94
-1.30
0.16
1.05
-0.19
0.08
-0.88
-0.36
-1.41
-0.78
-0.63
3.67
1.32
-0.66
-0.06
-1.65
-0.05
-1.21
1
1
0
1
0
0
1
1
1
1
1
1
0
0
1
1
0
0
1
0
0
0
0
0
1
0
1
0
15
15
15
15
15
15
15
15
15
15
15
15
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
17
38
40
30
35
35
27
25
35
19
15
6.4
16
35
40
38
34
38
25
53
16
38
24
47
17
41
33
59
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
LAWKADLLETIQTR
RFSGEVFER
VNLAPFSEELRQR
DLLPANSVNPMTR
TQFGYHVILK
EALCYQMARVSYR + Acrylamide (C)
VSDSDLRDFYNK
SLIRNMGTGSSPVLGR
QFLPKADPEIFAALK
SLPKDMHILQIAGR
STENTEDIQGCDSDKHGWYR + Acrylamide (C)
FYETTGGR
SFENAPPFIPHDTEGLVPITK
AAGTVSFEQAKPEIEQAVKMEK
TVGVHLEPMGVYSKK + Oxidation (M)
NVSTGDVNVEMNAAPGVDLTQLLNNMR + 2 Oxidation (M)
TVAASLFK
LLKDIISLDEGDNINK
TVGVHLEPMGVYSK
CSPTDEIPQK + Carbamidomethyl (C)
GDMLYFPSNLPKPLR + Oxidation (M)
YICDNQDTISSK + Carbamidomethyl (C)
ISIYVDNK
VKDLATVYVDAVK
VSWTSTGAPEYALYR
GDAGMKPSTEGVADYLSHNESKL + Oxidation (M)
523
812
755
444
528
837
692
708
359
286
299
80
763
306
720.8719
632.6473
882.4637
438.8943
483.9362
647.6492
843.9546
567.2940
618.3094
577.8359
584.2982
435.7739
887.9521
586.3319
1439.7292
1894.9201
1762.9128
1313.6611
1448.7868
1939.9258
1685.8946
1698.8602
1234.6042
1153.6572
1166.5818
869.5332
1773.8896
1170.6492
1439.7343
1894.9214
1762.9142
1313.6589
1448.7850
1939.9244
1685.8948
1698.8617
1234.6016
1153.6567
1166.5819
869.5334
1773.8872
1170.6469
-3.54
-0.68
-0.74
1.67
1.23
0.70
-0.08
-0.90
2.15
0.44
-0.04
-0.23
1.38
2.01
0
1
0
1
1
1
1
0
0
1
0
0
0
1
14
14
14
14
14
14
14
14
14
14
13
13
13
13
47
49
42
44
31
50
38
49
45
28
35
58
68
39
1
1
1
1
1
1
1
1
1
1
1
1
1
1
THLLFNHCLTNK
HFGKDFTPELQASYQK
ITAFFPGEAAVEVDIGK
HVSRAGGAEQFR
VVKVYGPAFACPK + Acrylamide (C)
IFNCVSDRAVTLDGMAR + Carbamidomethyl (C); Oxidation (M)
VGSGLITNKGNDEAIAK
YAASSYLTLTPAQWK
IFNCSPDQIK + Acrylamide (C)
SRLPGIVAEGR
LLGEYDAATSK
VVSLSIPR
EIGCHYIQFAPIVER
ASVVEARNGLR
936
539
722
889
158
685
29
370
674
767.0631
735.4324
857.4489
1021.5050
500.2261
559.2691
402.1835
624.8377
555.2863
2298.1675
1468.8502
1712.8832
2040.9954
998.4376
1674.7855
802.3524
1247.6608
1662.8371
2298.1678
1468.8501
1712.8767
2040.9972
998.4379
1674.7850
802.3497
1247.6609
1662.8312
-0.14
0.11
3.84
-0.86
-0.25
0.31
3.39
-0.01
3.55
0
1
1
1
0
0
0
1
1
13
13
13
13
13
13
13
13
13
43
13
52
53
21
43
7.5
65
69
1
1
1
1
1
1
1
1
1
IKALVDAVLSPDTK
NHMIISIDAEKALDK + Oxidation (M)
MSCLNKENYVVLSNNVK + Acrylamide (C); Oxidation (M)
DVDGAYMTK
GSYDGVDHDVNTIGVK
DSSYFGK
IKSEVSSELEK
AISESSTLEELKNDK
12/9/2009
Page 22 of 26
486
948
819
928
170
385
431
427
653
832
171
691.8445
803.0773
953.5180
738.6995
505.2691
630.2974
433.9117
646.8553
547.9609
964.9618
505.7066
1381.6744
2406.2101
1905.0214
2213.0767
1008.5236
1258.5802
1298.7133
1291.6960
1640.8609
1927.9090
1009.3986
1381.6767
2406.2100
1905.0281
2213.0708
1008.5249
1258.5830
1298.7095
1291.6958
1640.8603
1927.9107
1009.3966
-1.65
0.01
-3.51
2.66
-1.21
-2.18
2.90
0.16
0.36
-0.85
2.04
1
1
0
1
0
0
0
1
1
0
0
13
13
13
13
13
13
13
13
13
13
12
59
63
38
57
54
40
41
48
59
54
12
1
1
1
1
1
1
1
1
1
1
1
MNMQTIIEKMK + Oxidation (M)
AAGTVSFEQAKPEIEQAVKMEK + Oxidation (M)
VMDAASGTFIVLLTIPNK + Oxidation (M)
SVAPCEGTGYEIGSMSIIKGAK + Oxidation (M)
MVVAICAFR
ASSFDQGSSFVK
AVHHLEPIDIR
VRFPIVADMTK + Oxidation (M)
FFVAPFPEVFGKEK
ILYGCTFALLCHGMTR + 2 Carbamidomethyl (C); Oxidation (M)
MVCMGGCPR + Carbamidomethyl (C)
428
554
596
476
570
256
818
309
728
31
109
279
898
82
648.7668
745.4219
773.8785
683.8458
755.8645
556.3167
636.0141
587.7983
860.9462
403.7109
467.7307
574.2833
1042.0080
437.2189
1295.5190
1488.8292
1545.7424
1365.6770
1509.7144
1110.6188
1905.0205
1173.5820
1719.8778
805.4072
933.4468
1146.5520
2082.0014
872.4232
1295.5176
1488.8334
1545.7432
1365.6823
1509.7167
1110.6145
1905.0142
1173.5778
1719.8753
805.4083
933.4477
1146.5492
2082.0027
872.4239
1.15
-2.80
-0.47
-3.84
-1.49
3.87
3.29
3.60
1.49
-1.28
-0.94
2.52
-0.58
-0.79
0
0
1
1
1
1
1
0
0
0
0
0
1
0
12
12
12
12
12
12
12
12
12
12
12
12
12
12
8.4
36
55
68
60
42
39
76
76
59
63
66
65
38
1
1
1
1
1
1
1
1
1
1
1
1
1
1
WSSCPGMGHYR + Oxidation (M)
GLIMGGAVVIVTTSR + Oxidation (M)
CRAIFTMYIADR + Acrylamide (C); Oxidation (M)
MLRNSLGGESFR
NEEMMVKLSTQGK + Oxidation (M)
NVPGRPKTDK
RAGSTVQATYACLAVVPK + Acrylamide (C)
IEASSFQGAHK
IIEAGDEPMYTAAIVK
FVGDGGVR
VEGAPPMQYR
LAEQLEGN
412
705
909
774
580
485
37
720
348
339
732
456
931
65
302
700
152
663
743
71
167
124
614
202
714
808
781
840
639.3100
849.4444
720.0471
900.4890
762.3973
691.8167
404.7317
571.6414
616.3089
608.8502
577.2429
449.5939
761.7314
425.7609
584.8085
845.4277
496.7919
829.4658
581.6176
428.7785
502.7740
478.2532
789.4119
517.2692
850.9448
941.4268
605.6296
972.9593
1276.6054
1696.8742
2157.1195
1798.9634
1522.7800
1381.6188
807.4488
1711.9024
1230.6032
1215.6858
1728.7069
1345.7599
2282.1724
849.5072
1167.6024
1688.8408
991.5692
1656.9170
1741.8310
855.5424
1003.5334
954.4918
1576.8092
1032.5238
1699.8750
1880.8390
1813.8670
1943.9040
1276.6023
1696.8705
2157.1178
1798.9651
1522.7809
1381.6184
807.4491
1711.9039
1230.6027
1215.6823
1728.7009
1345.7606
2282.1729
849.5072
1167.5996
1688.8410
991.5702
1656.9172
1741.8311
855.5429
1003.5338
954.4957
1576.8097
1032.5240
1699.8749
1880.8356
1813.8669
1943.9081
2.49
2.18
0.75
-0.94
-0.54
0.35
-0.25
-0.90
0.48
2.92
3.44
-0.51
-0.22
0.02
2.44
-0.07
-0.98
-0.10
-0.10
-0.56
-0.38
-4.00
-0.26
-0.15
0.07
1.81
0.05
-2.08
0
1
1
0
0
0
0
1
0
1
1
0
0
1
1
0
0
1
1
0
0
0
1
0
0
1
0
0
12
12
12
12
12
12
12
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
70
59
52
54
88
55
97
62
85
80
14
44
74
44
71
89
72
47
79
23
1.5e+002
75
1.1e+002
1.6e+002
76
58
86
70
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
CAFINGPYAHK + Carbamidomethyl (C)
SPLTPKSPVPAEMESK
DASKLPSEGPQPAHVVVGDVR
IMTMLLIMEAVDK + Oxidation (M)
MDQGEIYLDGNK
ISSVGAFK
IRDLLPANSVNPMTR + Oxidation (M)
LQEDAQVGCLR
LVAVTVSDKER
ERGEGTCQDEETYR + Carbamidomethyl (C)
VSIAPLSVFGTQK
KTVFLSR
AELSHKEAQR
SYIQQNEAYLFVSK
ATTLQAFIK
QLTARHLSAHGLTPR
WATSEEGQKFIGDFK
ILALAVEK
DLDLYLPR
DVTLPEALRESGYK
VAPLSDEFR
CNAIAAAVLMPADVVR + Acrylamide (C); Oxidation (M)
CDGEEQTRVSLNCTQK + Acrylamide (C)
LGEDCNADILFVHSPK + Carbamidomethyl (C)
LTPDNMTGHELVDMLSR + Oxidation (M)
27
976
721
121
269
943
839
560
871
393
9
817
175
510
399.1928
973.4708
857.4075
475.7285
564.2775
799.7465
970.9710
749.8593
1006.4890
633.3223
362.6971
953.5174
507.8134
708.3563
796.3710
2917.3906
1712.8004
949.4424
1126.5404
2396.2177
1939.9274
1497.7040
2010.9634
1264.6300
723.3796
1905.0202
1013.6122
1414.6980
796.3715
2917.3909
1712.8006
949.4440
1126.5416
2396.2159
1939.9197
1497.7001
2010.9642
1264.6299
723.3803
1905.0142
1013.6121
1414.6953
-0.59
-0.12
-0.06
-1.60
-1.00
0.76
4.00
2.66
-0.38
0.08
-0.87
3.17
0.18
1.96
0
1
1
1
1
1
0
0
0
0
0
0
0
1
11
11
11
10
10
10
10
10
10
10
10
10
10
10
43
82
81
60
83
95
1.2e+002
85
95
1.4e+002
76
61
50
96
1
1
1
1
1
1
1
1
1
1
1
1
1
1
DYLSSGR
YGVASYPTSDLKEFLPGELPDEDFTK
EGYEAQISQDGNKFK
MFDPRER
MFWAMRTGK
LSGGFFKSQVMGLSTHILDTSR + Oxidation (M)
MIESDELSSGEVGYVVAR
ENGVWYIYGNQR
VMTEPELLSTACEQFLGK + Oxidation (M)
ENVTVSGFGDIK
AITEYK
NQNVLCVPAGELHVTIK + Acrylamide (C)
IADIASAILK
KYEIADNHAAQR
666
282
515
688
801
620
914
81
598
553.3139
577.3626
712.8866
842.8997
621.3305
792.9191
726.3399
436.7747
776.8179
1656.9199
1152.7106
1423.7586
1683.7848
1860.9697
1583.8236
2175.9979
871.5348
1551.6212
1656.9199
1152.7091
1423.7572
1683.7886
1860.9694
1583.8269
2175.9922
871.5378
1551.6190
-0.00
1.32
1.05
-2.24
0.17
-2.07
2.59
-3.44
1.45
0
1
1
1
1
0
1
1
0
10
10
10
10
10
10
10
10
10
55
16
78
1.1e+002
1.2e+002
83
79
58
16
1
1
1
1
1
1
1
1
1
QLYLQELLGLSQPR
LRVLIVGNGGR
HHLEKEYSILR
ALTTDTAAYGGLRADPLR
MADLFLITGYVTPK + Oxidation (M)
SSTVTASQTKCCSLSCSLSAR + Carbamidomethyl (C)
ELKGILSL
MSGGTMCPSYMVTR + 2 Oxidation (M)
12/9/2009
Page 23 of 26
387
664
665
919
340
455
583
838
779
468
626
632.7738
553.3138
553.3138
731.6721
608.8508
670.8496
765.3653
970.9705
602.6592
681.9025
798.3813
1263.5330
1656.9196
1656.9196
2191.9945
1215.6870
1339.6846
1528.7160
1939.9264
1804.9558
1361.7904
1594.7480
1263.5336
1656.9239
1656.9134
2191.9932
1215.6897
1339.6884
1528.7133
1939.9244
1804.9530
1361.7918
1594.7517
-0.41
-2.62
3.75
0.59
-2.17
-2.82
1.81
1.04
1.51
-1.02
-2.30
0
0
1
0
0
0
1
1
0
1
1
10
10
10
10
10
10
10
10
10
9
9
30
61
61
80
1.2e+002
1.1e+002
84
1.3e+002
1e+002
33
1.1e+002
1
1
1
1
1
1
1
1
1
1
1
TCRPEEECR + 2 Acrylamide (C)
LFSPPVVAALYLDPR
CPVQLIFALRQNGK + Acrylamide (C)
VGNMNPHWMGGFNTTLSWK + Oxidation (M)
LVGINETVIMK
QHVIPEIDAYR
HLCSYAFVDYVRG
IFNCVSDRAVTLDGMAR + Carbamidomethyl (C); Oxidation (M)
TSSGVISKPSQNISATTK
KIVEIPGEVPPGK
DVCVGITEKMCLER
770
312
586
672
188
895
921
268
447
769
910
884
703
897
897.9111
590.7678
765.4197
830.9406
512.2404
1029.5020
733.3522
564.2549
659.8359
598.6595
723.0724
678.7124
848.9625
690.3246
1793.8076
1179.5210
1528.8248
1659.8666
1022.4662
2056.9894
2197.0348
1126.4952
1317.6572
1792.9567
2166.1954
2033.1154
1695.9104
2067.9520
1793.8036
1179.5199
1528.8283
1659.8654
1022.4669
2056.9921
2197.0395
1126.4933
1317.6564
1792.9505
2166.1963
2033.1130
1695.9090
2067.9507
2.25
1.01
-2.25
0.73
-0.61
-1.31
-2.14
1.71
0.61
3.43
-0.43
1.17
0.86
0.63
1
1
1
1
0
1
0
1
1
0
1
1
1
1
9
9
9
9
9
9
9
9
9
9
9
9
9
9
1.3e+002
76
1.2e+002
1.4e+002
1.6e+002
1.3e+002
1.2e+002
59
1.8e+002
89
41
50
96
95
1
1
1
1
1
1
1
1
1
1
1
1
1
1
CGERTLPCCK + Acrylamide (C)
VDGVKLLIEMEQR
EFQNTAGEK
TQMDAFLSAARDIISGSMK + Oxidation (M)
YSLNLLDSSCLQCPDSAIK + Acrylamide (C); Carbamidomethyl (C)
MQKCSLCGGK + Carbamidomethyl (C); Oxidation (M)
KEALWTEQEGK
LAGPPLMLVTNEANPTR
RTWFGIPLVHINIGPGPHR
GGINSGIPADLIRNRPDIR
IRDLLPANSVNPMTR
FVGDGGVRMPEDCVPTFR + Acrylamide (C); Oxidation (M)
459
656
595
499
753
494
915
707
332
32
880
503
800
568
903
683
877
938
460
881
249
483
944
238
624
15
93
849
675.8493
822.9311
770.3958
701.8185
587.9742
695.8459
1089.0070
566.6379
604.8112
403.7112
673.3651
703.4064
927.4636
754.8756
697.6886
837.8938
673.3643
769.6688
451.2413
673.3652
553.2633
690.8613
799.7469
549.2560
532.2802
376.2379
452.7202
655.7100
1349.6840
1643.8476
1538.7770
1401.6224
1760.9008
1389.6772
2175.9994
1696.8919
1207.6078
805.4078
2017.0735
1404.7982
1852.9126
1507.7366
2090.0440
1673.7730
2017.0711
2305.9846
1350.7021
2017.0738
1104.5120
1379.7080
2396.2189
1096.4974
1593.8188
750.4612
903.4258
1964.1082
1349.6827
1643.8420
1538.7762
1401.6203
1760.9026
1389.6736
2176.0075
1696.8930
1207.6098
805.4082
2017.0700
1404.7990
1852.9170
1507.7374
2090.0466
1673.7719
2017.0772
2305.9801
1350.7030
2017.0700
1104.5130
1379.7119
2396.2118
1096.4978
1593.8151
750.4640
903.4232
1964.1142
1.02
3.45
0.52
1.54
-1.01
2.65
-3.69
-0.67
-1.62
-0.50
1.73
-0.53
-2.37
-0.52
-1.26
0.69
-3.03
1.92
-0.72
1.87
-0.83
-2.77
2.96
-0.31
2.31
-3.61
2.93
-3.05
1
0
1
1
1
1
1
1
0
1
1
1
1
0
1
1
1
0
1
1
0
1
1
0
0
1
0
1
9
9
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
1.7e+002
1.5e+002
1.3e+002
80
1.6e+002
1.6e+002
1.1e+002
1.3e+002
1.6e+002
1.3e+002
1e+002
57
1.7e+002
2.1e+002
1.7e+002
1.3e+002
1.3e+002
48
1.2e+002
1.2e+002
1.4e+002
1.9e+002
1.6e+002
1.3e+002
1.8e+002
1.1e+002
1e+002
32
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
DGKVYLNVEDAK
DGQALFVHAIASPYR
LLREYQDLMNTK + Oxidation (M)
MCESCLRSFAK + Acrylamide (C); Carbamidomethyl (C)
SRVGATGLMQVMPATAR + Oxidation (M)
TIIIEDNRSTDN
MMKLNVGEMHSDLEQVQR + 2 Oxidation (M)
NPVVEKINEGMQALR
NGTVIHWPER
FARQGEV
LIMSGNVLKVMVSNDIER
AAVRVPWPVPASR
MEDLIMKMLPQTIMK + 2 Oxidation (M)
TNVASLCELNMLK + Carbamidomethyl (C); Oxidation (M)
MTAGRAYEAETLAHEVITK
AFTNYEEVRDMTAK
FQDISELLFPIVDPEKK
MSTLLDFCFCTLTSCYMPK + Acrylamide (C); 2 Oxidation (M)
EELNYLDKSLK
LIMSGNVLKVMVSNDIER
CIPNNGIMML
NVSPMYKIIGDK + Oxidation (M)
ETGPMAVASANKVGQPAAVTAEAAR
YYGYTGAFR
LSFDGANYTLQPIR
TYKIVK
AMAEAANAR
SRLGFLAAAVAAMLPIHAR
317
343
901
151
464
64
290
835
682
657
918
226
905
752
598.3433
614.3325
522.8176
496.7209
452.9481
425.7605
580.7390
965.4526
835.9390
548.9641
1096.0130
539.2752
698.7072
587.9741
1194.6720
1226.6504
2087.2413
991.4272
1355.8225
849.5064
1159.4634
1928.8906
1669.8634
1643.8705
2190.0114
1076.5358
2093.0998
1760.9005
1194.6761
1226.6506
2087.2466
991.4281
1355.8249
849.5072
1159.4638
1928.8873
1669.8572
1643.8752
2190.0159
1076.5396
2093.0966
1760.8945
-3.39
-0.15
-2.55
-0.81
-1.76
-0.92
-0.28
1.74
3.76
-2.88
-2.04
-3.52
1.52
3.41
0
1
1
0
1
1
1
1
0
1
1
1
1
0
8
8
8
8
8
8
8
8
7
7
7
7
7
7
1e+002
1.6e+002
6.3
54
19
1e+002
30
1.9e+002
1.8e+002
1.8e+002
1.5e+002
2.7e+002
1.3e+002
2.3e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
TLAAHFVLQPV
AKDPDLLEAQK
LQSLIARVEALHVVILDAK
MDDTPLER + Oxidation (M)
QIRSLTGLLSLR
TKVFSIR
TDKCMEEHP + Acrylamide (C)
CVPKDHIDSNWLMAER + Oxidation (M)
YPAMVAASVFGMVALK + Oxidation (M)
LRHSVICIQCVFR + Acrylamide (C)
DCSENIIVFCGVKFMAESAK
LANQMRSNK + Oxidation (M)
SDVIELDMFVESIKVEIK
ISVEDTVNHLSYVASK
836
706
768
711
972
46
263
798
971
645.6769
849.4526
897.4855
567.6124
945.4654
414.7616
560.8340
616.6695
945.4647
1934.0089
1696.8906
1792.9564
1699.8154
2833.3744
827.5086
1119.6534
1846.9867
2833.3723
1934.0109
1696.8865
1792.9570
1699.8206
2833.3779
827.5116
1119.6540
1846.9823
2833.3705
-1.04
2.44
-0.33
-3.05
-1.24
-3.62
-0.46
2.39
0.63
1
1
0
0
1
0
0
1
1
7
7
7
7
7
7
7
7
7
1.7e+002
1.7e+002
1.4e+002
2.2e+002
2.1e+002
1.5e+002
45
1.3e+002
2e+002
1
1
1
1
1
1
1
1
1
QQYGEGIATAVKQTLEAK
RIPHTQAMGMLLTGR + Oxidation (M)
SPSIADEQTPAIPLNIK
WDHFINEVIEAAEK
LLEGIADFVTADGANMLKVWMSHGDK + Oxidation (M)
AIVLSTPK
LTLDGFILTK
LCPTQIGTKQSTTTGLK + Acrylamide (C)
GCGVEIQTFNDDVPGYIPADVLERR + Acrylamide (C)
12/9/2009
Page 24 of 26
88
958
970
917
655
693
671
466
239
912
300
448.7180
811.4171
896.4644
731.0106
822.4388
563.2930
830.9402
453.2418
550.2450
1088.0160
584.3209
895.4214
2431.2295
2686.3714
2190.0100
1642.8630
1686.8572
1659.8658
1356.7036
1098.4754
2174.0174
1166.6272
895.4222
2431.2231
2686.3676
2190.0085
1642.8638
1686.8599
1659.8654
1356.7078
1098.4764
2174.0244
1166.6295
-0.85
2.64
1.40
0.66
-0.48
-1.61
0.25
-3.09
-0.88
-3.20
-1.94
0
1
0
0
1
0
1
0
0
0
1
7
7
7
7
7
7
7
6
6
6
6
2.5e+002
2.7e+002
1.8e+002
1.7e+002
2.4e+002
2.7e+002
2.3e+002
2.8e+002
69
2.2e+002
1.6e+002
1
1
1
1
1
1
1
1
1
1
1
VVFTDCR + Carbamidomethyl (C)
EDGKAAGTVSFEQAKPEIEQAVK
QGQEFLLMLPQENPDIFNAELIK
NGVEMPITEAVYAVCHEGAK + Carbamidomethyl (C); Oxidation (M)
VTNQQAIDTARDLAK
LPCGHILHFHCLR + 2 Acrylamide (C)
IYAYLSDPFIR
GQVDTASYCR
LPLMGLVFACAVSMTSCGTK + 2 Carbamidomethyl (C); 2 Oxidation (M)
TNKYSLSQVK
742
77
879
673
935
887
955
649
878
380
911
816
807
960
581.6172
432.7491
1009.5440
832.4257
767.0630
680.3204
808.0443
818.4274
1009.5430
627.3644
725.6791
953.5166
936.9854
822.7275
1741.8298
863.4836
2017.0734
1662.8368
2298.1672
2037.9394
2421.1111
1634.8402
2017.0714
1252.7142
2174.0155
1905.0186
1871.9562
2465.1607
1741.8239
863.4865
2017.0666
1662.8400
2298.1678
2037.9459
2421.1053
1634.8416
2017.0652
1252.7139
2174.0161
1905.0207
1871.9491
2465.1580
3.35
-3.30
3.38
-1.87
-0.27
-3.21
2.39
-0.85
3.07
0.27
-0.29
-1.10
3.80
1.08
1
0
1
0
1
1
1
1
1
1
0
1
1
1
6
6
6
6
6
6
6
6
6
6
6
6
6
6
2.4e+002
4e+002
1.7e+002
3.4e+002
2.4e+002
2.3e+002
2.3e+002
3.9e+002
2e+002
1.2e+002
2.4e+002
1.8e+002
3.2e+002
2.6e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
EGSYAMGATRMQTALR
VSLAVGYR
SRTLSGWDSLLSIVQMPK
SGEGLSVVAPFAGMGLR + Oxidation (M)
CNITRISYNISDQVVEFLK + Carbamidomethyl (C)
AKGMENGAMLATDLGEATAR + 2 Oxidation (M)
NRVLMGSMHTGLEEYPDGVER + 2 Oxidation (M)
GRQSVLEPQYFSPK
IEMEEIVIETLKANELK + Oxidation (M)
GEALPASVLRAGI
SVEDNSPAMEAGLQSGDVIQK
ELNVDIVIEGTGRYVTK
NLYPGEKVYEFICGLK
KNDHFVHVIGTHLQAEDSMCGK
904
966
527
851
381
969
974
968
199
537
622
584
957
680
264
497
719
967
762
939
295
883
893
634
907
625
965
920
697.6901
875.4471
723.8706
983.0622
627.7693
880.1411
968.1389
880.1406
516.3136
733.8099
794.8644
765.4193
811.4146
834.9492
561.2604
699.2877
856.9485
875.4488
590.7018
770.0047
582.3532
509.2861
1022.9950
802.4194
703.3817
797.9167
875.4465
549.0848
2090.0485
2623.3195
1445.7266
1964.1098
1253.5240
2637.4015
2901.3949
2637.4000
1030.6126
1465.6052
1587.7142
1528.8240
2431.2220
1667.8838
1120.5062
1396.5608
1711.8824
2623.3246
1769.0836
2306.9923
1162.6918
2033.1153
2043.9754
1602.8242
2107.1233
1593.8188
2623.3177
2192.3101
2090.0466
2623.3098
1445.7263
1964.1142
1253.5243
2637.4047
2901.3910
2637.4088
1030.6135
1465.6040
1587.7174
1528.8184
2431.2264
1667.8883
1120.5084
1396.5652
1711.8788
2623.3237
1769.0815
2307.0008
1162.6962
2033.1093
2043.9791
1602.8222
2107.1313
1593.8225
2623.3271
2192.3184
0.88
3.68
0.25
-2.20
-0.19
-1.23
1.34
-3.35
-0.82
0.86
-1.99
3.69
-1.84
-2.65
-1.90
-3.14
2.14
0.34
1.17
-3.71
-3.70
2.93
-1.81
1.29
-3.83
-2.27
-3.58
-3.80
1
1
0
1
0
1
1
0
1
0
1
0
1
0
0
0
1
1
1
1
1
1
1
1
1
0
1
1
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3.4e+002
2.8e+002
3.8e+002
62
1e+002
1.5e+002
2.7e+002
1.6e+002
49
63
1.8e+002
3.3e+002
3.7e+002
2.5e+002
1.7e+002
45
2.8e+002
3e+002
7.6
1.3e+002
77
1.4e+002
3.6e+002
5.1e+002
2.3e+002
4.1e+002
3.7e+002
12
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
DWASDLQIIIDVAMSKGGR + Oxidation (M)
MSAKHPVIAVTGSSGAGTTTTSLMFR + Oxidation (M)
GGPGSINVISNFER
SRLGFLAAAVAAMLPIHAR
CWCLEGSVIM + 2 Carbamidomethyl (C)
MIGQIELITVPDVEAAEEKVIANR
YSINYIPLGCCRLSWNCANGMILR + 2 Acrylamide (C)
DVVVGDVCLLEPGEIIPVDGIFLR + Acrylamide (C)
TRLTGLVSGK
SCMICYNEFGVK + Carbamidomethyl (C); Oxidation (M)
LHTSPVMPPANIPR
DVLNMDIDSLNNTLKPKVDGSK + Oxidation (M)
SPLPLSPDSPATFLAR
MFTTQNSHR
SWMNCPAHDHL + Acrylamide (C); Oxidation (M)
SNGHGGTMLRVLGAAQK + Oxidation (M)
ENYNLLVVDDATCLIEGIMLKNK + Oxidation (M)
VRVIIEFLGGLDVIVK
QTPPCSSECPAGEDIRGYQR + 2 Carbamidomethyl (C)
VVDKYISIVK
LKEPVIMTIPPCFSFLK + Acrylamide (C)
ADMQMFTEMLVRIGNLK + 3 Oxidation (M)
MPMITITLPDGSRR + Oxidation (M)
EPLETPIKDGILYQQHVK
AEVAWNLAVAMLYK + Oxidation (M)
ILKDELQLTMALCGCASIADISTK + Acrylamide (C); Oxidation (M)
QGLDVIPKILIVTFLIPDAK
941
441
446
20
963
594
378
858
686
891
876
947
872
850
780.0287
656.7878
657.8906
385.2032
837.7535
769.3241
627.2770
991.5092
840.8812
511.8112
505.0611
802.7133
1006.4890
655.7101
2337.0643
1311.5610
1313.7666
768.3918
2510.2387
1536.6336
1252.5394
1981.0038
1679.7478
2043.2157
2016.2153
2405.1181
2010.9634
1964.1085
2337.0670
1311.5621
1313.7667
768.3919
2510.2453
1536.6328
1252.5394
1980.9985
1679.7526
2043.2206
2016.2097
2405.1277
2010.9647
1964.1142
-1.18
-0.82
-0.02
-0.02
-2.62
0.53
0.04
2.68
-2.84
-2.41
2.77
-3.98
-0.60
-2.91
1
0
1
1
1
0
0
1
0
0
0
1
1
1
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3.3e+002
1.4e+002
1.6e+002
1.2e+002
4.3e+002
67
2.3e+002
4.3e+002
2.5e+002
19
12
4e+002
4.8e+002
92
1
1
1
1
1
1
1
1
1
1
1
1
1
1
GIRAECADCHIPQEGWYYVK
IEGSCCCGTVK + 3 Acrylamide (C)
RIIDDILTLSR
WKGIEH
MMTGRVLLVCALCVLWCVAGGR + Acrylamide (C); Carbamidomethyl (C); 2 Oxidation (M)
EDAGSWVSGESEER
DFLGCAEDDIR
SPLAHAEMLCLAAGAARSR + Carbamidomethyl (C)
DDGSEVELTAEPGSFK
ALVPFILLACLPTLFVVSK
EIPGFLSLLAIFVMLIIK
MSKMQLESSSVDLNGMITQFK + 2 Oxidation (M)
ALLNGPGNYSFNKATDDSK
ALLRIAVVWNIPVACNR + Carbamidomethyl (C)
950
68
973
934
959
885
962
882
474
603.7581
428.2611
951.1422
1150.0900
822.7272
678.7128
837.7533
509.2854
682.7771
2411.0033
854.5076
2850.4048
2298.1654
2465.1598
2033.1166
2510.2381
2033.1125
1363.5396
2410.9949
854.5086
2850.3936
2298.1608
2465.1571
2033.1165
2510.2365
2033.1058
1363.5393
3.49
-1.14
3.90
2.02
1.08
0.02
0.63
3.29
0.26
0
1
1
0
1
0
1
0
0
3
3
3
3
3
3
3
3
3
91
2.2e+002
5.7e+002
5e+002
5e+002
2.2e+002
5.6e+002
2.6e+002
81
1
1
1
1
1
1
1
1
1
CPPIEAFTASGGHSAMAACCMAR + 2 Carbamidomethyl (C); Oxidation (M)
RVPALSGR
YFLRNSVNQSYSLAVDGGSELVNYR
LAMMIGTAGFTAMLCVIALER + Acrylamide (C); Oxidation (M)
NGTAHNETQHDLSTDKAQLSWL
LSALPDCLLHAIMSLLPAR
VLKQSCAMLNAGQITVVCAETMK + Carbamidomethyl (C); Oxidation (M)
AIGQGFAGGLKPLAEAHGVAIG
MDEVCCVHPGMK + Oxidation (M)
12/9/2009
Page 25 of 26
379
977
150
916
283
436
610
886
964
806
401
627.3524
984.8216
494.3006
1096.0120
577.7849
654.3399
787.3110
509.3176
870.7753
936.4973
635.2741
1252.6902
2951.4430
986.5866
2190.0094
1153.5552
1306.6652
1572.6074
2033.2413
2609.3041
1870.9800
1268.5336
1252.6888
2951.4519
986.5873
2190.0052
1153.5588
1306.6678
1572.6085
2033.2435
2609.2972
1870.9788
1268.5312
1.18
-3.04
-0.63
1.95
-3.07
-1.99
-0.69
-1.09
2.62
0.65
1.96
1
1
1
0
1
0
0
0
0
0
0
3
3
3
3
2
2
2
2
2
2
2
2.8e+002
5.3e+002
2.6e+002
4.6e+002
8.9e+002
8.9e+002
40
11
6.3e+002
5.4e+002
2.3e+002
1
1
1
1
1
1
1
1
1
1
1
GIGAPVDRELAR
MSQVVRALEGDVSLDDLNEGVRPGHSR + Oxidation (M)
VVKNTLASR
WSMSADDPYPQPVVGLAEGR + Oxidation (M)
ADAERSAAHAR
GAIIIMFFCHR
NEHYNMMSDFNR + Oxidation (M)
GVLTVVALVAGMLVVGLGLPR
ANLENIYLAAGSNYPDALSGSALASK
ESLWNQAIQTAEQILK
MVCQCNTEIGR + Oxidation (M)
913
501
606
518
930
766
894
90
115
101
902
772
336
230
544.8313
702.2787
785.3226
476.6246
566.8438
892.8857
684.0735
450.2745
472.2874
458.7875
697.6881
450.0286
606.7360
543.2208
2175.2961
1402.5428
1568.6306
1426.8520
2263.3461
1783.7568
2049.1987
898.5344
942.5602
915.5604
2090.0425
1796.0853
1211.4574
1084.4270
2175.2952
1402.5394
1568.6282
1426.8507
2263.3384
1783.7595
2049.1921
898.5349
942.5610
915.5575
2090.0506
1796.0923
1211.4587
1084.4252
0.41
2.44
1.54
0.86
3.38
-1.51
3.20
-0.45
-0.83
3.19
-3.90
-3.93
-1.02
1.66
1
0
1
1
0
0
1
1
1
1
1
1
0
0
2
2
1
1
1
1
1
1
1
1
1
1
0
0
41
48
1e+002
1.1e+002
29
3.1e+002
67
1e+003
7.9e+002
2.4e+002
1e+003
34
54
1.1e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
TLAIVYIQEILTILSKMVK
MPGADDCWTDHR
ERVACYHCGCESR + Carbamidomethyl (C)
ANTILKVATEIVR
SVLPLIITLFFFFLQQPIK
ETLESEQGASSTSESDK
LRHLLPSFMVPSTLLAVR
VVRSSVPR
RQISSKPK
KPIKSCLK
EALDEAGFKHIPIMAYSAK
NPKILISFLNATLKPK
CEMEDAYHAK + Oxidation (M)
FGCTCPACQR
4
5
8
19
38
128
168
181
236
498
500
525
542
552
575
587
592
599
605
612
645
659
660
668
675
676
687
701
357.6368
357.6369
362.2222
384.2352
406.2485
480.8010
502.8138
509.3178
546.8398
467.6240
702.2767
482.2989
491.2992
496.9743
505.9746
765.8278
511.6497
777.8378
784.3622
526.3250
541.0008
412.7505
550.0006
829.4681
417.0087
555.6760
561.3514
564.6759
713.2590
713.2592
722.4298
766.4558
810.4824
959.5874
1003.6130
1016.6210
1091.6650
1399.8502
1402.5388
1443.8749
1470.8758
1487.9011
1514.9020
1529.6410
1531.9273
1553.6610
1566.7098
1575.9532
1619.9806
1646.9729
1646.9800
1656.9216
1664.0057
1664.0062
1681.0324
1691.0059
702
716
717
739
740
745
746
771
784
785
794
795
804
805
423.7591
428.0154
570.3517
579.3513
434.7656
439.0218
585.0267
599.7021
456.7780
608.7023
614.3776
461.0352
467.7850
623.3776
1691.0073
1708.0325
1708.0333
1735.0321
1735.0333
1752.0581
1752.0583
1796.0845
1823.0829
1823.0851
1840.1110
1840.1117
1867.1109
1867.1110
809
810
822
823
833
834
842
844
845
472.0416
629.0533
478.7914
638.0533
643.7283
483.0485
487.3049
489.7975
652.7286
1884.1373
1884.1381
1911.1365
1911.1381
1928.1631
1928.1649
1945.1905
1955.1609
1955.1640
12/9/2009
855
856
866
892
896
906
908
929
658.4028
494.0548
500.8045
682.0799
516.0678
527.0740
533.8240
555.8371
Page 26 of 26
1972.1866
1972.1901
1999.1889
2043.2179
2060.2421
2104.2669
2131.2669
2219.3193
Search Parameters
Type of search
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
MS/MS Ion Search
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
979
12/9/2009
Page 1 of 22
User
Email
Search title
MS data file
Database
Timestamp
Protein hits
:
:
:
:
:
:
:
LTQFT Daemon
D:\Data\Mark\18_7-09\N_18_7_09_9.RAW
9 Dec 2009 at 04:15:27 GMT
gi|157165273 CjaC [Campylobacter concisus 13826]
gi|157164629 elongation factor Tu [Campylobacter concisus 13826]
gi|38683423 endopin 2B [Bos taurus]
gi|157165649 DNA-binding response regulator [Campylobacter concisus 13826]
gi|157164319 elongation factor Ts [Campylobacter concisus 13826]
gi|157164740 outer membrane fibronectin-binding protein [Campylobacter concisus 13826]
gi|154175426 enoyl-(acyl carrier protein) reductase [Campylobacter curvus 525.92]
gi|157164610 translocation protein TolB [Campylobacter concisus 13826]
gi|136429
gi|121531628 SERPINA3-4 [Bos taurus]
gi|157164830 lipopolysaccharide biosynthesis protein [Campylobacter concisus 13826]
gi|122614
gi|194038347 PREDICTED: alpha-1-antichymotrypsin 3 [Sus scrofa]
gi|28317
gi|291474
gi|1942778
gi|32267367 putative C4-dicarboxylatebinding periplasmic protein [Helicobacter hepaticus ATCC 51449]
gi|2232299
gi|157165029 branched-chain amino acid aminotransferase [Campylobacter concisus 13826]
gi|122411
gi|223039637 DNA-binding response regulator [Campylobacter rectus RM3267]
gi|1245695
gi|154175542 membrane protein [Campylobacter curvus 525.92]
gi|157163930 outer membrane protein [Campylobacter concisus 13826]
gi|157164579 cysteine synthase A [Campylobacter concisus 13826]
gi|157164268 ketol-acid reductoisomerase [Campylobacter concisus 13826]
gi|157165110 hydrogenase accessory protein HypB [Campylobacter concisus 13826]
gi|6320157
gi|157164144 thioredoxin-disulfide reductase [Campylobacter concisus 13826]
gi|157165776 putative carbon storage regulator-like protein [Campylobacter concisus 13826]
gi|158604943 aspartate-semialdehyde dehydrogenase [Campylobacter concisus 13826]
gi|157164277 glyceraldehyde-3-phosphate dehydrogenase, type I [Campylobacter concisus 13826]
gi|157164748 methyl-accepting chemotaxis protein [Campylobacter concisus 13826]
gi|157165527 hydrogenase-4 component I [Campylobacter concisus 13826]
gi|157165509 DNA-directed RNA polymerase subunit alpha [Campylobacter concisus 13826]
gi|119916467 PREDICTED: similar to serpin peptidase inhibitor, clade B like [Bos taurus]
gi|66046197 histidine kinase, HAMP region: chemotaxis sensory transducer [Pseudomonas syringae pv. syringae B728a]
gi|181402
gi|157163909 serine hydroxymethyltransferase [Campylobacter concisus 13826]
gi|157164709 peptidoglycan associated lipoprotein [Campylobacter concisus 13826]
gi|225632
casein alphaS1
gi|27806941 serine proteinase inhibitor, clade A, member 1 [Bos taurus]
gi|157164923 peptide methionine sulfoxide reductase family protein [Campylobacter concisus 13826]
gi|157164833 phosphopyruvate hydratase [Campylobacter concisus 13826]
gi|154174678 outer membrane fibronectin-binding protein [Campylobacter curvus 525.92]
gi|157164023 carbon-nitrogen family hydrolase [Campylobacter concisus 13826]
100
2
2.00 %
179
2
1.12 %
12/9/2009
Page 2 of 22
Format As
Standard scoring
Show sub-sets 0
Require bold red
Show pop-ups
Select All
1.
Help
Peptide Summary
Select None
MudPIT scoring
Suppress pop-ups
Search Selected
Error tolerant
Archive Report
gi|157165109
Mass: 35585
Score: 630
Queries matched: 23
emPAI: 1.91
Query
181
182
199
214
215
224
241
243
251
397
409
494
510
593
608
809
812
827
835
836
871
892
893
2.
Observed
501.3248
501.3248
509.3221
517.7687
517.7689
527.3183
542.2695
542.7839
549.3031
636.3610
644.3581
721.4097
735.8651
813.3508
827.4077
1081.5140
1089.5090
744.0426
1144.5980
763.4012
896.7938
1021.1390
1026.4720
Mr(expt)
1000.6350
1000.6350
1016.6296
1033.5228
1033.5232
1052.6220
1082.5244
1083.5532
1096.5916
1270.7074
1286.7016
1440.8048
1469.7156
1624.6870
1652.8008
2161.0134
2177.0034
2229.1060
2287.1814
2287.1818
2687.3596
3060.3952
3076.3942
Mr(calc)
1000.6355
1000.6355
1016.6304
1033.5233
1033.5233
1052.6230
1082.5244
1083.5535
1096.5917
1270.7067
1286.7017
1440.8048
1469.7150
1624.6887
1652.8006
2161.0110
2177.0059
2229.1066
2287.1808
2287.1808
2687.3588
3060.3982
3076.3931
ppm Miss Score
Expect Rank
-0.40
0
57 7.3e-005
1
-0.40
0
(54) 0.00015
1
-0.71
0
(52) 0.00031
1
-0.42
0
39
0.28
1
-0.04
0
(21)
18
2
-0.88
0
23
1.1
1
0.02
0
34
0.31
1
-0.23
1
20
9
1
-0.05
0
38
0.14
1
0.56
0
(31)
0.58
1
-0.01
0
32
0.68
1
0.02
1
36
0.16
1
0.41
0
55
0.0037
1
-0.99
0
37
0.06
1
0.15
1
47
0.019
1
1.15
0
46
0.022
1
-1.11
0
(28)
1.3
1
-0.28
0
27
2.5
1
0.28
0
82 5.9e-006
1
0.42
0
(43)
0.047
1
0.28
1
21
7.6
1
-1.00
1
(34)
0.27
1
0.33
1
37
0.12
1
gi|157165273
Mass: 40059
Score: 592
CjaC [Campylobacter concisus 13826]
Queries matched: 21
Peptide
R.VLLMLALTK.T
R.VLLMLALTK.T
R.VLLMLALTK.T + Oxidation (M)
K.IQEFFLTH.K.IQEFFLTH.K.LIAAVPDINK.I
K.TNTTGVDTFK.S
K.YYMNPLRK.H
K.IGTVFYGNVK.Y
K.SDKPVVLVGAMR.N
K.SDKPVVLVGAMR.N + Oxidation (M)
K.LANRINELLNSGK.A
K.YGFLTSDNLNAQK.A
R.VGSGETTMNGEVDDAK.Y + Oxidation (M)
K.GEQISNIGSQDMNNEVWLK.L
K.GEQISNIGSQDMNNEVWLK.L + Oxidation (M)
R.VDIIYSHANDNPDFVNVAVK.N
R.NSGSLSADGPLNLFNAVNVAISK.D
K.IATIKGEQISNIGSQDMNNEVWLK.L
R.VGSGETTMNGEVDDAKYGFLTSDNLNAQK.A
R.VGSGETTMNGEVDDAKYGFLTSDNLNAQK.A + Oxidation (M)
emPAI: 0.89
Query
149
151
176
273
274
286
294
311
336
422
Observed
486.7553
487.7794
498.2633
563.7834
563.7837
571.7811
576.3082
586.3393
609.8276
653.3379
Mr(expt)
971.4960
973.5442
994.5120
1125.5522
1125.5528
1141.5476
1150.6018
1170.6640
1217.6406
1304.6612
Mr(calc)
971.4964
973.5444
994.5124
1125.5529
1125.5529
1141.5478
1150.6022
1170.6648
1217.6404
1304.6612
-0.33
0
40
0.09
1
-0.16
1
47
0.026
1
-0.33
0
24
5.8
1
-0.54
0
(43)
0.065
1
-0.01
0
(44)
0.06
1
-0.11
0
68 0.00013
1
-0.35
0
29
1.7
1
-0.68
0
27
2.1
1
0.21
0
44
0.069
1
0.04
1
62 0.00084
1
Peptide
K.ISDYAYIK.T
K.SVDDIKGLK.I
K.VFDPYNIK.I
K.SPFAMLDFAK.S
K.SPFAMLDFAK.S
K.SPFAMLDFAK.S + Oxidation (M)
R.IPGFGGEIYAK.L
K.SFPPEVIAALK.K
K.AIFEAAAAEVAR.D
K.NAYEKLPDDLK.A
459
473
474
475
476
487
488
559
680
681
692.8102
474.2694
710.9011
710.9011
474.2700
717.3400
717.3407
787.3901
889.8984
889.8990
1383.6058
1419.7864
1419.7876
1419.7876
1419.7882
1432.6654
1432.6668
1572.7656
1777.7822
1777.7834
1383.6064
1419.7874
1419.7874
1419.7874
1419.7874
1432.6663
1432.6663
1572.7671
1777.7835
1777.7835
-0.39
-0.73
0.16
0.16
0.53
-0.60
0.38
-0.93
-0.71
-0.03
R.AGNTGMQMGGWFK.K
K.IRIPGFGGEIYAK.L
K.IRIPGFGGEIYAK.L
K.IRIPGFGGEIYAK.L
K.IRIPGFGGEIYAK.L
K.VFYANVEYWDK.M
K.VFYANVEYWDK.M
K.ISDYAYIKTNEEK.K.SGQYDIGYTASYYYK.G
K.SGQYDIGYTASYYYK.G
0
1
1
1
1
0
0
1
0
0
53
(38)
(37)
(30)
50
(23)
51
42
56
(52)
0.0037
0.11
0.13
0.67
0.0067
3.8
0.006
0.081
0.0016
0.0035
1
1
1
1
1
1
1
1
1
1
12/9/2009
682
3.
889.9008
1777.7870
1777.7835
1.99
0
(32)
0.33
1
Page 3 of 22
K.SGQYDIGYTASYYYK.G
gi|157163977
Mass: 36762
Score: 587
Queries matched: 21
emPAI: 1.17
4.
Query
24
25
Observed
385.1974
385.1975
Mr(expt)
768.3802
768.3804
Mr(calc)
768.3806
768.3806
54
129
134
278
280
287
349
441
442
452
499
501
502
523
578
603
604
605
823
421.2261
473.7511
477.7556
564.3138
564.3141
572.7960
619.8043
675.3469
675.3477
683.3453
485.2691
727.4004
727.4006
744.8608
801.9097
548.3047
821.9540
548.3054
740.0665
840.4376
945.4876
953.4966
1126.6130
1126.6136
1143.5774
1237.5940
1348.6792
1348.6808
1364.6760
1452.7855
1452.7862
1452.7866
1487.7070
1601.8048
1641.8923
1641.8934
1641.8944
2217.1777
840.4382
945.4879
953.4971
1126.6135
1126.6135
1143.5772
1237.5938
1348.6809
1348.6809
1364.6758
1452.7864
1452.7864
1452.7864
1487.7085
1601.8049
1641.8938
1641.8938
1641.8938
2217.1794
ppm Miss Score
-0.50
0
(36)
-0.24
0
45
-0.60
-0.30
-0.43
-0.40
0.14
0.25
0.17
-1.23
-0.04
0.16
-0.64
-0.11
0.17
-0.99
-0.06
-0.90
-0.19
0.38
-0.77
0
1
1
0
0
0
0
0
0
0
1
1
1
0
0
1
1
1
1
Expect Rank
0.046
1
0.0063
1
32
0.35
54
0.0056
24
4.8
(37)
0.18
54
0.0037
35
0.39
64 0.00041
(20)
11
(61) 0.0011
61
0.0011
(20)
6.1
67
0.0001
(21)
4
37
0.18
21
9.1
(23)
3.9
69 0.00011
(22)
4.2
23
3.3
gi|157164629
Mass: 43586
Score: 574
Queries matched: 29
elongation factor Tu [Campylobacter concisus 13826]
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
1
2
1
Peptide
K.AADFFAK.R
K.AADFFAK.R
K.FYDVIGK.E
K.RAEELSGGK.I
K.GKAADFFAK.R
K.FAHVVAASTPK.G
K.FAHVVAASTPK.G
K.VQDGAVGEELK.G
K.QALSEATAYER.E
K.LGNVQMAAPSFSK.F
K.LGNVQMAAPSFSK.F
K.LGNVQMAAPSFSK.F + Oxidation (M)
K.FYDVIGKELIEK.T
K.FYDVIGKELIEK.T
K.FYDVIGKELIEK.T
K.GFVALDYWDAGFK.H
K.VQVFPSAQLLDDDR.V
K.VQDGAVGEELKGLVTK.K
K.VQVFPSAQLLDDDRVFGALK.L
emPAI: 1.58
Query
83
219
220
421
535
624
631
632
633
637
651
709
752
759
760
766
798
799
800
846
847
857
858
860
863
872
880
884
885
5.
Observed
434.2558
524.7382
524.7383
652.3823
755.4103
845.4077
566.3199
848.9775
848.9778
851.9070
859.9048
616.6545
975.4888
983.4866
983.4867
991.4848
703.0303
708.3610
708.3611
1190.0890
793.7287
840.7361
1260.6020
1268.5990
1276.5990
898.1454
940.8447
946.1758
946.1766
Mr(expt)
866.4970
1047.4618
1047.4620
1302.7500
1508.8060
1688.8008
1695.9379
1695.9404
1695.9410
1701.7994
1717.7950
1846.9417
1948.9630
1964.9586
1964.9588
1980.9550
2106.0691
2122.0612
2122.0615
2378.1634
2378.1643
2519.1865
2519.1894
2535.1834
2551.1834
2691.4144
2819.5123
2835.5056
2835.5080
Mr(calc)
866.4974
1047.4621
1047.4621
1302.7507
1508.8061
1688.8005
1695.9407
1695.9407
1695.9407
1701.7992
1717.7941
1846.9425
1948.9638
1964.9587
1964.9587
1980.9536
2106.0667
2122.0616
2122.0616
2378.1642
2378.1642
2519.1884
2519.1884
2535.1833
2551.1782
2691.4154
2819.5103
2835.5052
2835.5052
ppm Miss Score
Expect Rank
-0.38
0
24
2.3
1
-0.27
0
30
0.54
1
-0.08
0
(27)
1.2
1
-0.50
0
77 6.6e-006
1
-0.06
0
38
0.13
1
0.18
1
36
0.26
1
-1.65
0
(41)
0.033
1
-0.13
0
57
0.001
1
0.22
0
(26)
0.91
1
0.15
0
64
0.00037
1
0.54
0
(48)
0.013
1
-0.43
1
24
4.7
1
-0.39
0
(25)
3.6
1
-0.04
0
48
0.021
1
0.06
0
(43)
0.065
1
0.71
0
(38)
0.19
1
1.13
1
(31)
0.79
1
-0.21
1
36
0.3
1
-0.07
1
(25)
3.3
1
-0.30
0
52
0.0073
1
0.05
0
(24)
4.6
1
-0.76
0
(30)
1.1
1
0.42
0
31
0.8
1
0.06
0
(21)
7.9
1
2.05
0
(21)
6
1
-0.37
0
34
0.23
1
0.69
1
23
1.7
1
0.12
1
(20)
4.9
1
0.97
1
(23)
2.5
1
gi|157165374
Mass: 33156
Score: 574
Queries matched: 17
Peptide
R.EHILLSR.Q
K.AGQDGEWSAK.I
K.AGQDGEWSAK.I
K.TTLTAAISAVLSR.K
R.QVGVPYIVVFMNK.A + Oxidation (M)
K.ALEEAKAGQDGEWSAK.I
R.ISVELIAPVALEEGTR.F
K.EMDQGEAGDNVGVLLR.G
K.EMDQGEAGDNVGVLLR.G + Oxidation (M)
R.GITIATSHIEYETEKR.H
K.IMELMDAVDSYIPTPVR.A
K.IMELMDAVDSYIPTPVR.A + Oxidation (M)
K.IMELMDAVDSYIPTPVR.A + Oxidation (M)
K.IMELMDAVDSYIPTPVR.A + 2 Oxidation (M)
R.ATDKDLLMPIEDVFSISGR.G
R.ATDKDLLMPIEDVFSISGR.G + Oxidation (M)
R.ATDKDLLMPIEDVFSISGR.G + Oxidation (M)
R.ELLNEYNFPGDDTPIVSGSALK.A
R.TTDVTGSITLPEGTEMVMPGDNVR.I
R.TTDVTGSITLPEGTEMVMPGDNVR.I + Oxidation (M)
R.TTDVTGSITLPEGTEMVMPGDNVR.I + 2 Oxidation (M)
K.VGDTIEIVGIKPTQTTTVTGVEMFR.K
K.VGDTIEIVGIKPTQTTTVTGVEMFRK.E
K.VGDTIEIVGIKPTQTTTVTGVEMFRK.E + Oxidation (M)
K.VGDTIEIVGIKPTQTTTVTGVEMFRK.E + Oxidation (M)
emPAI: 1.36
Query
67
302
340
460
538
615
Observed
426.2346
582.7858
614.2982
692.8328
756.8806
838.3996
Mr(expt)
850.4546
1163.5570
1226.5818
1383.6510
1511.7466
1674.7846
Mr(calc)
850.4549
1163.5571
1226.5819
1383.6518
1511.7468
1674.7850
ppm Miss Score
Expect Rank
-0.26
1
23
7.9
1
-0.03
1
22
7.7
3
-0.04
0
21
9.1
1
-0.54
0
88 1.5e-006
1
-0.07
1
42
0.067
1
-0.19
0
53
0.0046
1
Peptide
K.AKGFDVSK.L
K.FDGAKDENLR.S
K.AILDNFDEYK.T
K.DSGLTSDLSSFAGK.K
K.DSGLTSDLSSFAGKK.Y
12/9/2009
652
653
744
775
778
779
789
790
802
803
814
6.
573.6744
860.0084
970.4806
994.5285
670.9952
1005.9910
1034.5280
690.0213
713.6953
1070.0400
731.3957
1718.0014
1718.0022
1938.9466
1987.0424
2009.9638
2009.9674
2067.0414
2067.0421
2138.0641
2138.0654
2191.1653
1718.0018
1718.0018
1938.9469
1987.0414
2009.9694
2009.9694
2067.0419
2067.0419
2138.0644
2138.0644
2191.1637
-0.27
0.24
-0.15
0.51
-2.82
-0.99
-0.21
0.09
-0.15
0.49
0.73
0
0
0
1
0
0
1
1
1
1
1
(47)
0.0023
50
0.0014
74 4.8e-005
26
2.5
(25)
2.9
65
0.00032
58
0.0018
(23)
5.7
(23)
5.8
31
1
20
5.8
Page 4 of 22
1
1
1
1
1
1
1
1
1
1
1
R.SVVAIYPELLAFVVAK.D
K.CSVQSTGGSVYNVNNVLK.K + Acrylamide (C)
K.AILDNFDEYKTLHPALK.S
K.KEDLVQGLSAPLHPAAEAAFK.E
gi|27807517
Mass: 42533
Score: 441
Queries matched: 17
emPAI: 0.45
Query
109
192
208
233
250
256
261
317
318
443
486
496
600
629
630
644
826
7.
Observed
454.7452
507.7185
515.7165
532.7958
549.2903
554.2714
555.2545
590.3601
590.3601
675.8490
714.3689
722.3665
818.8925
566.3100
848.9615
571.6415
744.0213
Mr(expt)
907.4758
1013.4224
1029.4184
1063.5770
1096.5660
1106.5282
1108.4944
1178.7056
1178.7056
1349.6834
1426.7232
1442.7184
1635.7704
1695.9082
1695.9084
1711.9027
2229.0421
Mr(calc)
907.4763
1013.4236
1029.4186
1063.5774
1096.5665
1106.5284
1108.4938
1178.7063
1178.7063
1349.6835
1426.7238
1442.7188
1635.7709
1695.9090
1695.9090
1711.9039
2229.0410
-0.52
0
34
0.59
7
-1.18
0
63 0.00011
1
-0.11
0
(60) 0.00016
1
-0.35
1
28
1.6
1
-0.44
0
56
0.0024
1
-0.16
0
33
0.61
1
0.61
0
28
0.8
1
-0.57
0
56 0.00044
1
-0.57
0
(48) 0.0028
1
-0.07
0
20
13
1
-0.41
0
(23)
5.8
1
-0.21
0
32
0.77
1
-0.25
0
23
5.1
1
-0.49
1
46
0.017
1
-0.32
1
(32)
0.46
1
-0.73
1
(25)
2.6
1
0.46
0
21
6.6
1
gi|194676990
Mass: 45494
Score: 439
Queries matched: 12
Peptide
R.DTQYLLR.T
R.ADFSGMSSGR.G
R.ADFSGMSSGR.G + Oxidation (M)
R.RDTQYLLR.T
K.HINTWVAEK.T
K.TYDFLSSFK.D
K.GNWDTQFNK.E
R.LVLVNAIYFK.G
R.LVLVNAIYFK.G
K.NVEKPVQMMFK.K
R.DLLPANSVNPMTR.L
R.DLLPANSVNPMTR.L + Oxidation (M)
K.GNTAAQMCQTLSLNK.S + Carbamidomethyl (C)
K.IRDLLPANSVNPMTR.L
K.IRDLLPANSVNPMTR.L
K.IRDLLPANSVNPMTR.L + Oxidation (M)
K.SSGGGEDVHQGFQNLLSEVNR.R
emPAI: 0.32
Query
9
53
92
114
166
175
205
238
239
335
675
853
8.
Observed
357.6982
417.7050
439.7527
465.7662
493.7975
497.8057
513.8007
540.2958
540.2958
608.3035
884.4128
831.3988
Mr(expt)
713.3818
833.3954
877.4908
929.5178
985.5804
993.5968
1025.5868
1078.5770
1078.5770
1214.5924
1766.8110
2491.1746
gi|62126072
Mass: 46687
Mr(calc)
713.3820
833.3953
877.4909
929.5182
985.5808
993.5971
1025.5869
1078.5771
1078.5771
1214.5931
1766.8112
2491.1754
-0.24
0
22
3.9
1
0.20
0
35
0.31
1
-0.05
0
35
0.37
1
-0.37
0
37
0.4
1
-0.34
0
54
0.0042
1
-0.26
0
38
0.038
1
-0.06
1
31
0.62
1
-0.04
0
47
0.023
1
-0.04
0
(44)
0.041
1
-0.57
0
39
0.11
1
-0.06
0
67 0.00016
1
-0.34
1
33
0.5
1
Score: 422
Queries matched: 12
Peptide
R.NSLQPR.H
K.MQDLEAK.L
K.LINEYVK.N
K.LTPETLTR.W
R.DILSQLGIK.K
K.LAVSQVIHK.A
K.QLALKDPNK.N
R.HIDELSLPR.F
R.HIDELSLPR.F
R.FSISSDYQLR.D
K.DAEAFYASEVLSTNFKDSEAAVK.L
emPAI: 0.15
Query
9
53
92
114
175
205
236
238
239
285
335
675
9.
Observed
357.6982
417.7050
439.7527
465.7662
497.8057
513.8007
358.8759
540.2958
540.2958
568.3218
608.3035
884.4128
Mr(expt)
713.3818
833.3954
877.4908
929.5178
993.5968
1025.5868
1073.6059
1078.5770
1078.5770
1134.6290
1214.5924
1766.8110
Mr(calc)
713.3820
833.3953
877.4909
929.5182
993.5971
1025.5869
1073.6089
1078.5771
1078.5771
1134.6285
1214.5931
1766.8112
-0.24
0
22
3.9
1
0.20
0
35
0.31
1
-0.05
0
35
0.37
1
-0.37
0
37
0.4
1
-0.26
0
38
0.038
1
-0.06
1
31
0.62
1
-2.85
1
24
3.5
1
-0.04
0
47
0.023
1
-0.04
0
(44)
0.041
1
0.51
0
48
0.016
1
-0.57
0
39
0.11
1
-0.06
0
67 0.00016
1
gi|157164497
Mass: 29815
Score: 393
Queries matched: 11
Peptide
R.NSLQPR.H
K.MQDLEAK.L
K.LINEYVK.N
K.LTPETLTR.W
K.LAVSQVIHK.A
K.QLALKDPNK.N
R.VKVPMMTLR.L
R.HIDELSLPR.F
R.HIDELSLPR.F
K.ETQSIIFLGK.V
R.FSISSDYQLR.D
emPAI: 0.70
12/9/2009
10.
Page 5 of 22
Query
137
291
381
398
448
491
511
558
590
591
Observed
479.7350
573.8113
630.3400
637.8597
678.8815
719.8732
736.3910
783.9216
811.4377
811.4380
Mr(expt)
957.4554
1145.6080
1258.6654
1273.7048
1355.7484
1437.7318
1470.7674
1565.8286
1620.8608
1620.8614
Mr(calc)
957.4556
1145.6081
1258.6656
1273.7030
1355.7483
1437.7324
1470.7679
1565.8274
1620.8610
1620.8610
-0.14
0
22
3.4
1
-0.01
0
49
0.023
1
-0.12
0
70 0.00014
1
1.44
1
27
2
2
0.14
1
22
4.3
1
-0.41
0
51
0.011
1
-0.28
0
36
0.26
1
0.80
1
33
0.55
1
-0.09
1
(24)
5.1
1
0.28
1
44
0.03
1
Peptide
K.GYTEGYLR.K
K.SVVAEPLFER.K
K.LEDELLELASK.T
R.KSVVAEPLFER.K
K.MLVPADGIEGVKK.V
K.NTTNNTPAVIHTR.I
R.KNTTNNTPAVIHTR.I
R.YAKLEDELLELASK.T
R.YAKLEDELLELASK.T
598
817.9332
1633.8518
1633.8523
-0.25
K.AQTTESSSLGKDILGK.I
1
39
0.17
1
gi|158605017
Mass: 30046
Score: 357
Queries matched: 15
emPAI: 0.69
Query
32
33
41
146
172
186
428
572
573
575
576
588
621
622
697
11.
Observed
398.2031
398.2033
404.7317
485.2611
496.7925
504.2741
657.8382
799.8976
534.2935
800.9380
800.9385
807.8953
844.4721
844.4722
605.6295
Mr(expt)
794.3916
794.3920
807.4488
968.5076
991.5704
1006.5336
1313.6618
1597.7806
1599.8587
1599.8614
1599.8624
1613.7760
1686.9296
1686.9298
1813.8667
gi|38683423
Mass: 46972
endopin 2B [Bos taurus]
Mr(calc)
794.3923
794.3923
807.4490
968.5080
991.5702
1006.5335
1313.6615
1597.7810
1599.8620
1599.8620
1599.8620
1613.7759
1686.9305
1686.9305
1813.8669
-0.81
0
(31)
1.2
1
-0.30
0
34
0.64
1
-0.23
1
20
13
6
-0.33
1
32
0.72
1
0.24
1
21
8.6
1
0.15
1
29
1.7
1
0.24
1
43
0.057
1
-0.23
0
66 0.00026
1
-2.11
0
(27)
1.8
1
-0.37
0
47
0.022
1
0.25
0
(34)
0.39
1
0.07
0
(44)
0.041
1
-0.49
1
35
0.21
1
-0.38
1
(32)
0.39
1
-0.12
0
32
0.67
1
Score: 337
Queries matched: 9
Peptide
K.GVTFTDR.G
K.GVTFTDR.G
K.SIADKFK.G
K.DGIKFFSR.G
K.QSFELIKK.D
K.VEKEFVEK.G
R.GTYIKYEANQK.G
R.NAVMYNDFVVIADK.S
K.LLDKPLFTPDANTR.K
K.LLDKPLFTPDANTR.K
K.LLDKPLFTPDANTR.K
emPAI: 0.23
Query
9
53
92
114
166
205
285
676
853
12.
Observed
357.6982
417.7050
439.7527
465.7662
493.7975
513.8007
568.3218
884.9022
831.3988
Mr(expt)
713.3818
833.3954
877.4908
929.5178
985.5804
1025.5868
1134.6290
1767.7898
2491.1746
Mr(calc)
713.3820
833.3953
877.4909
929.5182
985.5808
1025.5869
1134.6285
1767.7952
2491.1754
-0.24
0
22
3.9
1
0.20
0
35
0.31
1
-0.05
0
35
0.37
1
-0.37
0
37
0.4
1
-0.34
0
54 0.0042
1
-0.06
1
31
0.62
1
0.51
0
48
0.016
1
-3.02
0
42
0.038
1
-0.34
1
33
0.5
1
gi|115495209
Mass: 59194
Score: 303
Queries matched: 11
Peptide
R.NSLQPR.L
K.MQDLEAK.L
K.LINEYVK.N
K.LTPETLTR.W
K.DILSQLGIK.K
K.QLALKDPNK.N
K.ETQSIIFLGK.V
K.IFTSDADFSGITDDHK.L
emPAI: 0.24
Query
15
39
97
107
298
314
369
383
385
657
848
13.
Observed
367.2339
403.7114
443.7280
451.7256
580.7896
587.7974
626.2787
632.7740
633.2862
865.3610
804.6765
Mr(expt)
732.4532
805.4082
885.4414
901.4366
1159.5646
1173.5802
1250.5428
1263.5334
1264.5578
1728.7074
2411.0077
Mr(calc)
732.4534
805.4083
885.4419
901.4368
1159.5656
1173.5812
1250.5424
1263.5336
1264.5580
1728.7083
2411.0084
-0.21
0
49 0.0046
1
-0.04
0
28
1.3
1
-0.47
0
46 0.0095
1
-0.15
0
(27)
1
1
-0.78
0
47
0.016
1
-0.80
0
(22)
6.6
1
0.37
0
32
0.36
1
-0.10
0
29
0.36
1
-0.14
0
(25)
2.2
1
-0.50
1
22
1.1
1
-0.31
1
48 0.0032
1
gi|157165649
Mass: 40295
Score: 290
Queries matched: 7
DNA-binding response regulator [Campylobacter concisus 13826]
Peptide
R.FNLVLK.N
R.FVGDGGVR.M
R.FSVQMFK.F
R.FSVQMFK.F + Oxidation (M)
R.TCRPEEECR.L + 2 Acrylamide (C)
emPAI: 0.27
Query
268
436
498
518
543
Observed
557.8451
668.3141
727.3666
740.8458
759.3853
Mr(expt)
1113.6756
1334.6136
1452.7186
1479.6770
1516.7560
Mr(calc)
1113.6757
1334.6136
1452.7184
1479.6769
1516.7562
-0.08
1
27
0.78
1
0.00
0
21
6.8
2
0.20
0
60 0.00098
1
0.08
0
67 0.00015
1
-0.11
0
21
9.1
1
Peptide
K.AILESGKLVGK.C
R.DNSILGCGTGDVK.A + Carbamidomethyl (C)
K.AIYTWVANTMQR.D
K.EFSYDYVSTELK.K.GYGWIPVDPADVTK.V
12/9/2009
545
888
14.
764.9201
969.8556
1527.8256
2906.5450
1527.8256
2906.5430
0.01
0.69
1
0
40
55
0.087
0.0014
gi|157164319
Mass: 38441
Score: 274
Queries matched: 6
elongation factor Ts [Campylobacter concisus 13826]
1
1
Page 6 of 22
K.ALEIIGNTKGDLER.A
R.IWLPLPLSTTYQQLTQDYVINTTAK.N
emPAI: 0.28
Query
198
202
376
439
489
607
Observed
508.7797
511.2631
629.3245
671.3254
717.3672
826.9097
Mr(expt)
1015.5448
1020.5116
1256.6344
1340.6362
1432.7198
1651.8048
Mr(calc)
1015.5451
1020.5128
1256.6361
1340.6361
1432.7198
1651.8053
-0.21
0
45
0.071
1
-1.09
0
40
0.11
2
-1.30
0
43
0.042
1
0.13
0
54
0.0042
1
0.03
1
26
3.6
1
-0.28
0
66 0.00037
1
Peptide
R.NPQFQALAK.D
R.FELGEGLEK.K
K.DTTAHIQSSGIK.T
R.FYADNTVLDQR.L
K.KVDDFAAEVAAQIG.K.ATISEINSETDFVAR.N
gi|171855138
Mass: 39114
Score: 274
Queries matched: 6
RecName: Full=Elongation factor Ts; Short=EF-Ts
15.
gi|77736341
Mass: 52315
Score: 274
Queries matched: 6
emPAI: 0.20
Query
110
143
295
403
579
726
Observed
456.2688
481.7487
577.8355
641.3094
801.9149
939.9434
Mr(expt)
910.5230
961.4828
1153.6564
1280.6042
1601.8152
1877.8722
Mr(calc)
910.5236
961.4828
1153.6567
1280.6037
1601.8161
1877.8717
-0.61
0
52
0.0037
1
-0.00
0
58
0.0017
1
-0.25
1
26
1.5
1
0.42
0
44
0.1
1
-0.56
1
29
1.4
1
0.31
0
64 0.00041
1
gi|109940161
Mass: 52314
Score: 274
RecName: Full=Antithrombin-III; Short=ATIII;
gi|157838185
Mass: 48618
Score: 274
Chain A, Crystal Structure Of Cleaved Bovine
16.
Peptide
R.LPGIVAEGR.S
K.SSELVSANR.L
K.SRLPGIVAEGR.S
R.SDLYVSDAFHK.A
K.LQPLDFKGNAEQSR.L
K.EQLQDMGLEDLFSPEK.S
Queries matched: 6
Flags: Precursor
Queries matched: 6
Antithrombin Iii At 3.2 Angstroms Resolution
gi|157164740
Mass: 36430
Score: 266
Queries matched: 7
emPAI: 0.30
outer membrane fibronectin-binding protein [Campylobacter concisus 13826]
Query
46
61
108
423
534
625
626
17.
Observed
411.7392
422.7372
453.2633
653.3561
752.9222
564.3173
845.9730
Mr(expt)
821.4638
843.4598
904.5120
1304.6976
1503.8298
1689.9301
1689.9314
Mr(calc)
821.4647
843.4603
904.5130
1304.6976
1503.8297
1689.9301
1689.9301
-0.99
1
29
1.5
1
-0.50
0
28
0.84
3
-1.07
1
26
2.5
1
0.04
0
66 0.00029
1
0.12
1
57
0.0013
1
-0.03
0
(23)
3.3
1
0.79
0
62 0.00032
1
Peptide
K.YAAEIKK.V
R.YHANLVK.N
R.FALKAEAR.D
K.AVAEVLAGYGVEK.A
R.AKAVAEVLAGYGVEK.A
K.ISTVGYGELKPIATNK.T
gi|154175426
Mass: 28890
Score: 257
Queries matched: 5
emPAI: 0.39
enoyl-(acyl carrier protein) reductase [Campylobacter curvus 525.92]
Query
124
147
167
259
526
Observed
471.2920
485.2898
494.2665
554.2931
746.4065
Mr(expt)
940.5694
968.5650
986.5184
1106.5716
1490.7984
Mr(calc)
940.5706
968.5655
986.5185
1106.5720
1490.7981
ppm Miss Score
Expect Rank
-1.18
0
50
0.0081
1
-0.43
0
32
0.37
1
-0.06
0
24
5
2
-0.34
0
70
0.00015
1
0.26
0
81 7.3e-006
1
Peptide
K.GLIVGVANAK.S
R.VNAISAGPIK.T
R.YLAHDLGAK.N
K.TLAASGIGDFR.M
K.EGGSVLTLTYLGGPK.F
Mass: 29118
Score: 257
Queries matched: 5
gi|157165165
18.
gi|157164029
Mass: 49918
Score: 233
Queries matched: 8
emPAI: 0.29
Query
20
196
210
512
611
616
Observed
380.2339
508.3017
516.2997
736.4039
831.9177
839.9142
Mr(expt)
758.4532
1014.5888
1030.5848
1470.7932
1661.8208
1677.8138
Mr(calc)
758.4538
1014.5896
1030.5845
1470.7930
1661.8195
1677.8145
ppm Miss Score
-0.72
0
31
-0.73
0
48
0.33
0
(36)
0.17
0
57
0.79
0
33
-0.36
0
(26)
Expect Rank
1.7
1
0.0082
1
0.3
5
0.002
1
0.71
1
3.1
1
Peptide
K.TDLAVIK.I
R.GGIMQGLVIK.R.GGIMQGLVIK.- + Oxidation (M)
K.ISPDTQGVLVTDVK.S
K.SGGNNGIGFAIPSNMVK.D
K.SGGNNGIGFAIPSNMVK.D + Oxidation (M)
12/9/2009
764
769
19.
986.4879
994.4842
1970.9612
1986.9538
1970.9619
1986.9568
-0.32
-1.49
0
0
64 0.00041
(27)
2.3
gi|157164610
Mass: 46159
Score: 224
Queries matched: 5
translocation protein TolB [Campylobacter concisus 13826]
Page 7 of 22
1
1
K.EGALISGVEQGMPADEAGLK.R
K.EGALISGVEQGMPADEAGLK.R + Oxidation (M)
emPAI: 0.23
20.
Query
325
Observed
601.3301
Mr(expt)
1200.6456
Mr(calc)
1200.6463
ppm Miss Score
-0.51
0
56
360
427
530
531
623.3639
657.3405
747.8845
749.3650
1244.7132
1312.6664
1493.7544
1496.7154
1244.7129
1312.6663
1493.7548
1496.7147
0.30
0.09
-0.23
0.49
gi|11935049
Mass: 66027
Score: 219
0
0
0
0
24
50
62
32
Expect Rank
0.0032
1
2.6
0.0098
0.00085
0.73
Queries matched: 6
Peptide
K.ELGGQSSLGVVR.L
1
1
1
1
K.TIVSGGLNIFPK.W
R.FSSDGQSVVFIK.E
R.YALEGSMGSPLTLR.V
K.DQPDIFIYNTGSK.N
emPAI: 0.05
Query
90
150
213
234
420
Observed
437.7536
487.2686
517.2615
533.2642
651.8612
Mr(expt)
873.4926
972.5226
1032.5084
1064.5138
1301.7078
Mr(calc)
873.4920
972.5240
1032.5087
1064.5138
1301.7078
ppm Miss Score
Expect Rank
0.77
0
26
6.6
3
-1.36
0
32
1.3
2
-0.27
0
26
2.7
1
0.04
0
31
1.3
1
0.02
0
74 4.1e-005
1
Peptide
R.SLVNLGGSK.S
K.IEISELNR.V
R.TLLEGEESR.M
K.AQYEDIAQK.S
R.SLDLDSIIAEVK.A
464
697.3697
1392.7248
1392.7249
-0.02
R.TNAENEFVTIKK.D
gi|119395750
Mass: 65999
Score: 219
gi|160961491
Mass: 65450
Score: 219
21.
gi|136429
Mass: 24394
Score: 208
1
31
0.83
1
Queries matched: 6
Queries matched: 6
Queries matched: 11
emPAI: 0.47
Query
57
58
59
217
218
818
819
820
821
833
838
Observed
421.7582
421.7582
421.7583
523.2852
523.2852
1106.0550
737.7061
737.7062
1106.0560
1142.0940
767.0624
Mr(expt)
841.5018
841.5018
841.5020
1044.5558
1044.5558
2210.0954
2210.0965
2210.0968
2210.0974
2282.1734
2298.1654
Mr(calc)
841.5022
841.5022
841.5022
1044.5564
1044.5564
2210.0967
2210.0967
2210.0967
2210.0967
2282.1729
2298.1678
-0.36
0
(39)
0.18
1
-0.36
0
56
0.0042
1
-0.12
0
(41)
0.11
1
-0.48
0
35
0.37
1
-0.48
0
(24)
5.2
1
-0.58
0
91
1e-006
1
-0.11
0
(63) 0.00063
1
0.02
0
(43)
0.057
1
0.33
0
(49)
0.015
1
0.25
0
(21)
8.4
1
-1.06
0
27
2.1
1
Peptide
R.VATVSLPR.S
R.VATVSLPR.S
R.VATVSLPR.S
K.LSSPATLNSR.V
K.LSSPATLNSR.V
K.IITHPNFNGNTLDNDIMLIK.L + Oxidation (M)
Mass: 23458
Score: 208
Queries matched: 11
gi|494360
gi|999627
Mass: 8814
Score: 208
Queries matched: 11
gi|1942351
Mass: 13284
Score: 208
Queries matched: 11
gi|2914482
Mass: 23460
Score: 208
Queries matched: 11
Mass: 23457
Score: 208
Queries matched: 11
gi|3318722
gi|110590762
Mass: 23458
Score: 208
Queries matched: 11
gi|157878102
Mass: 24157
Score: 208
Queries matched: 11
gi|242253868
Mass: 25865
Score: 208
Queries matched: 11
22.
gi|121531628
Mass: 46282
Score: 208
Queries matched: 6
emPAI: 0.15
Query
9
53
191
205
272
Observed
357.6982
417.7050
506.7949
513.8007
561.3135
Mr(expt)
713.3818
833.3954
1011.5752
1025.5868
1120.6124
Mr(calc)
713.3820
833.3953
1011.5753
1025.5869
1120.6128
-0.24
0
22
3.9
1
0.20
0
35
0.31
1
-0.04
0
21
6.8
1
-0.06
1
31
0.62
1
-0.33
0
51 0.0073
1
Peptide
R.NSLQPR.R
K.MQDLEAK.L
R.IHELYLPK.F
K.QLALKDPNK.N
K.DTQSIIFLGK.V
12/9/2009
440
673.8692
1345.7238
1345.7241
-0.21
gi|226373739
Mass: 46361
Score: 208
23.
1
49
0.016
1
Page 8 of 22
K.IEELFKDLSPR.T
Queries matched: 6
gi|157164830
Mass: 38482
Score: 208
Queries matched: 8
emPAI: 0.28
lipopolysaccharide biosynthesis protein [Campylobacter concisus 13826]
Query
174
193
245
348
362
363
365
366
24.
Observed
497.7472
507.7951
543.8056
619.3379
416.5989
624.3948
624.3953
624.3955
Mr(expt)
993.4798
1013.5756
1085.5966
1236.6612
1246.7749
1246.7750
1246.7760
1246.7764
Mr(calc)
993.4801
1013.5757
1085.5968
1236.6615
1246.7761
1246.7761
1246.7761
1246.7761
-0.26
0
33
0.58
1
-0.05
1
35
0.26
1
-0.16
0
45
0.034
1
-0.20
0
44
0.036
1
-1.00
0
(49) 0.00036
1
-0.86
0
(25)
0.11
1
-0.06
0
(36)
0.011
1
0.26
0
50 0.00037
1
Peptide
R.SITDMQGVK.V + Oxidation (M)
K.VSVPEKVEK.I
K.DLQIEELVK.L
K.NGFSAVNLIFR.D
K.VLHLLGGANLLK.V
K.VLHLLGGANLLK.V
K.VLHLLGGANLLK.V
K.VLHLLGGANLLK.V
gi|122614
Mass: 15998
Score: 198
Queries matched: 7
emPAI: 0.78
Query
386
399
400
485
776
781
843
Observed
633.4185
637.8658
637.8668
713.8463
1000.4650
1008.4620
776.0720
Mr(expt)
1264.8224
1273.7170
1273.7190
1425.6780
1998.9154
2014.9094
2325.1942
Mr(calc)
1264.8231
1273.7183
1273.7183
1425.6776
1998.9146
2014.9095
2325.1964
-0.51
0
50 0.00026
1
-0.98
0
(26)
2
1
0.59
0
40
0.08
1
0.32
0
21
8.4
1
0.43
0
64 0.00029
1
-0.03
0
(43)
0.033
1
-0.98
1
24
3.8
1
gi|255683515
Mass: 16129
Score: 198
25.
Peptide
R.LLGNVLVVVLAR.H
R.LLVVYPWTQR.F
R.LLVVYPWTQR.F
K.DFTPELQASYQK.V
K.AAVLALWDKVNEEEVGGEALGR.L
Queries matched: 7
gi|194038347
Mass: 46611
Score: 193
Queries matched: 6
PREDICTED: alpha-1-antichymotrypsin 3 [Sus scrofa]
emPAI: 0.07
Query
50
140
164
228
270
811
26.
Observed
417.2221
480.7790
493.7789
529.2802
558.8300
725.3749
Mr(expt)
832.4296
959.5434
985.5432
1056.5458
1115.6454
2173.1029
Mr(calc)
832.4299
959.5440
985.5444
1056.5465
1115.6451
2173.1015
gi|28317
Mass: 59492
Score: 192
-0.30
0
24
6.5
1
-0.57
0
49
0.015
1
-1.17
0
32
0.77
1
-0.58
1
27
1.9
2
0.32
1
31
0.58
1
0.62
1
29
1.3
1
Queries matched: 6
Peptide
K.VPMMSLR.G
R.IDALYLPR.F
K.QLVSLDPSK.N
R.WRDSLQPR.R
R.RIDALYLPR.F
K.VFTHEADLSGVTGDNKLGVSK.V
emPAI: 0.11
Query
40
211
345
382
457
642
Observed
404.2031
516.3025
617.8430
631.8016
691.3280
854.3904
Mr(expt)
806.3916
1030.5904
1233.6714
1261.5886
1380.6414
1706.7662
Mr(calc)
806.3923
1030.5910
1233.6717
1261.5899
1380.6408
1706.7649
-0.76
0
24
4.3
1
-0.55
0
29
0.69
1
-0.20
1
25
3.2
1
-0.96
0
29
0.97
1
0.44
0
46
0.032
1
0.79
0
40
0.077
1
Peptide
R.LAADDFR.L
R.VLDELTLTK.A
R.LKYENEVALR.Q
R.SLLEGEGSSGGGGR.G
R.ALEESNYELEGK.I
gi|21961605
Mass: 58792
Score: 192
Queries matched: 6
Mass: 62761
Score: 192
Queries matched: 6
gi|114667509
gi|114667511
Mass: 58244
Score: 192
Queries matched: 6
gi|114667513
Mass: 56692
Score: 192
Queries matched: 6
gi|119581085
Mass: 63308
Score: 192
Queries matched: 6
gi|195972866
Mass: 58766
Score: 192
Queries matched: 6
Mass: 58818
Score: 192
Queries matched: 6
gi|251757513
12/9/2009
Page 9 of 22
gi|114667507
Mass: 100282
Score: 191
Queries matched: 6
27.
gi|291474
Mass: 17423
Score: 190
Queries matched: 4
emPAI: 0.19
Query
209
297
330
Observed
516.2615
579.7764
604.3068
Mr(expt)
1030.5084
1157.5382
1206.5990
Mr(calc)
1030.5083
1157.5387
1206.5993
515
738.8682
1475.7218
1475.7216
0.11
0
40
0.11
1
-0.36
0
25
1.9
1
-0.21
1
21
9.8
1
0.15
0
104
4e-008
Peptide
K.YAASSYLTR.T
R.ASGVPDRFSGSK.S
1
K.VNDAVTTDGVQTTR.S
gi|291478
Mass: 22654
Score: 190
Queries matched: 4
28.
gi|1942778
Mass: 15089
Score: 176
Queries matched: 7
emPAI: 0.50
Query
216
225
226
246
290
392
532
Observed
521.2753
529.2728
529.2728
544.3163
573.3297
634.8585
750.3654
Mr(expt)
1040.5360
1056.5310
1056.5310
1086.6180
1144.6448
1267.7024
1498.7162
gi|146149083
Mass:
gi|146149272
Mass:
29.
Mr(calc)
1040.5365
1056.5314
1056.5314
1086.6186
1144.6452
1267.7024
1498.7164
-0.43
0
38
0.12
1
-0.35
0
(23)
4.7
1
-0.35
0
(27)
2
1
-0.49
1
21
12
1
-0.28
1
44
0.033
1
0.06
0
25
1.9
1
-0.13
0
48
0.015
1
15220
Score: 176
caballus]
15220
Score: 176
caballus]
Peptide
R.MFLGFPTTK.T
K.LRVDPVNFK.L
-.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEFGAEALER.M
Queries matched: 7
Queries matched: 7
gi|32267367
Mass: 37749
Score: 173
Queries matched: 9
emPAI: 0.29
putative C4-dicarboxylatebinding periplasmic protein [Helicobacter hepaticus ATCC 51449]
Query
24
25
134
262
263
477
478
479
480
30.
Observed
385.1974
385.1975
477.7556
555.3317
555.3317
474.9485
474.9486
711.9195
711.9199
Mr(expt)
768.3802
768.3804
953.4966
1108.6488
1108.6488
1421.8237
1421.8240
1421.8244
1421.8252
Mr(calc)
768.3806
768.3806
953.4971
1108.6492
1108.6492
1421.8242
1421.8242
1421.8242
1421.8242
-0.50
0
(36) 0.046
1
-0.24
0
45 0.0063
1
-0.43
1
24
4.8
1
-0.30
0
51 0.0052
1
-0.30
0
(41)
0.05
1
-0.36
1
(34) 0.084
1
-0.15
1
(36) 0.056
1
0.18
1
53
0.001
1
0.75
1
(39) 0.027
1
gi|119914036
Mass: 34009
Score: 172
Queries matched: 4
Peptide
K.AADFFAK.R
K.AADFFAK.R
K.GKAADFFAK.R
K.KPIIVPEDAK.G
K.KPIIVPEDAK.G
K.KPIIVPEDAKGQK.M
K.KPIIVPEDAKGQK.M
K.KPIIVPEDAKGQK.M
K.KPIIVPEDAKGQK.M
emPAI: 0.20
Query
9
53
440
785
31.
Observed
357.6982
417.7050
673.8692
1021.9880
Mr(expt)
713.3818
833.3954
1345.7238
2041.9614
Mr(calc)
713.3820
833.3953
1345.7241
2041.9633
-0.24
0
22
3.9
1
0.20
0
35
0.31
1
-0.21
1
49
0.016
1
-0.89
0
67 0.00021
1
gi|2232299
Mass: 47885
Score: 172
Queries matched: 9
Peptide
R.NSLQPR.K.MQDLEAK.L
K.IEELFKDLSPR.T
emPAI: 0.14
Query
249
258
387
389
596
597
684
693
694
Observed
547.2814
554.2890
633.8090
633.8091
545.2870
817.4279
595.3304
900.4896
900.4896
Mr(expt)
1092.5482
1106.5634
1265.6034
1265.6036
1632.8392
1632.8412
1782.9694
1798.9646
1798.9646
Mr(calc)
1092.5485
1106.5641
1265.6040
1265.6040
1632.8413
1632.8413
1782.9702
1798.9651
1798.9651
-0.24
0
44
0.058
1
-0.63
0
(29)
1.8
1
-0.46
0
35
0.38
1
-0.30
0
(30)
1.3
1
-1.29
0
(36)
0.36
1
-0.02
0
68 0.00022
1
-0.47
0
(22)
3.9
1
-0.27
0
(25)
2.8
1
-0.27
0
26
2.2
1
Peptide
K.ALETHTYFER.H
K.ALETHTYFER.H
K.GFAPADVFVQWLQR.G
K.GFAPADVFVQWLQR.G
12/9/2009
Page 10 of 22
gi|3834667
Mass: 48183
Score: 172
Queries matched: 9
gi|24496448
Mass: 65621
Score: 172
Queries matched: 9
Mass: 49106
Score: 172
Queries matched: 9
gi|33413902
gi|34538498
Mass: 49909
Score: 172
Queries matched: 9
32.
gi|157165029
Mass: 34028
Score: 170
Queries matched: 4
emPAI: 0.20
branched-chain amino acid aminotransferase [Campylobacter concisus 13826]
Query
148
301
411
671
33.
Observed
486.2583
582.3029
644.8325
882.3986
Mr(expt)
970.5020
1162.5912
1287.6504
1762.7826
Mr(calc)
970.5018
1162.5910
1287.6499
1762.7832
0.25
0
32
0.56
1
0.21
1
31
1.1
1
0.40
0
62 0.0007
1
-0.29
0
45
0.019
1
Peptide
K.LAPAAQMNR.A
K.KYESFLTYI.K.AYFDVVYGLNK.K
K.ASSNYLSSQMANYEAK.E
gi|122411
Mass: 15236
Score: 165
Queries matched: 8
emPAI: 0.22
Query
216
225
226
246
290
392
539
540
Observed
521.2753
529.2728
529.2728
544.3163
573.3297
634.8585
505.9106
758.3630
Mr(expt)
1040.5360
1056.5310
1056.5310
1086.6180
1144.6448
1267.7024
1514.7100
1514.7114
Mr(calc)
1040.5365
1056.5314
1056.5314
1086.6186
1144.6452
1267.7024
1514.7113
1514.7113
-0.43
0
38
0.12
1
-0.35
0
(23)
4.7
1
-0.35
0
(27)
2
1
-0.49
1
21
12
1
-0.28
1
44
0.033
1
0.06
0
25
1.9
1
-0.91
0
37
0.19
2
0.07
0
(31)
0.69
1
Peptide
R.MFLGFPTTK.T
K.LRVDPVNFK.L
M.VLSAADKTNVK.A
K.FLSSVSTVLTSK.Y
K.VGGHAGEYGAEALER.M
K.VGGHAGEYGAEALER.M
gi|230479
Mass: 15105
Score: 165
Queries matched: 8
gi|230632
Mass: 15105
Score: 165
Queries matched: 8
gi|313500
Mass: 15236
Score: 165
Queries matched: 8
Mass: 15105
Score: 165
Queries matched: 8
gi|24158717
34.
gi|47523844
Mass: 47164
Score: 163
Queries matched: 4
emPAI: 0.07
Query
197
264
571
756
35.
Observed
508.3108
555.8051
799.4225
982.4784
Mr(expt)
1014.6070
1109.5956
1596.8304
1962.9422
Mr(calc)
1014.6073
1109.5968
1596.8300
1962.9422
-0.28
0
37
0.15
1
-1.07
0
25
1.9
1
0.28
0
31
0.72
1
0.02
0
70 0.00012
1
gi|223039637
Mass: 39873
Score: 157
Queries matched: 4
DNA-binding response regulator [Campylobacter rectus RM3267]
Peptide
K.SLLGNLGITK.V
K.LTISGTYDLK.S
R.IAPNLADFAFSLYR.Q
emPAI: 0.27
36.
Query
268
431
Observed
557.8451
661.3063
Mr(expt)
1113.6756
1320.5980
Mr(calc)
1113.6757
1320.5980
436
498
668.3141
727.3666
1334.6136
1452.7186
1334.6136
1452.7184
ppm Miss Score
Expect Rank
-0.08
1
27
0.78
1
0.03
0
70 7.6e-005
1
0.00
0.20
0
0
(45)
60
0.024
0.00098
gi|1245695
Mass: 28553
Score: 157
Queries matched: 5
1
1
Peptide
K.AILESGKLVGK.C
R.DNSVIGCGTGDVK.A + Carbamidomethyl (C)
R.DNSVIGCGTGDVK.A + Acrylamide (C)
K.AIYTWVANTMQR.D
emPAI: 0.12
Query
5
9
114
191
272
Observed
352.6839
357.6982
465.7662
506.7949
561.3135
Mr(expt)
703.3532
713.3818
929.5178
1011.5752
1120.6124
Mr(calc)
703.3541
713.3820
929.5182
1011.5753
1120.6128
-1.20
0
26
1.8
1
-0.24
0
22
3.9
1
-0.37
0
37
0.4
1
-0.04
0
21
6.8
1
-0.33
0
51 0.0073
1
Peptide
K.TPFDPK.H
R.NSLQPR.R
K.LTPETLTR.W
R.IHELYLPK.F
K.DTQSIIFLGK.V
Mass: 46175
Score: 157
Queries matched: 5
gi|31340900
12/9/2009
Page 11 of 22
gi|160332365
Mass: 46208
Score: 157
Queries matched: 5
gi|226373737
Mass: 46208
Score: 157
Queries matched: 5
37.
gi|154175542
Mass: 22096
Score: 154
Queries matched: 3
membrane protein [Campylobacter curvus 525.92]
emPAI: 0.53
Query
327
424
514
Observed
602.8438
654.3513
737.8986
Mr(expt)
1203.6730
1306.6880
1473.7826
Mr(calc)
1203.6724
1306.6881
1473.7827
0.54
0
40
0.076
1
-0.06
1
48
0.016
1
-0.07
0
66 0.00029
1
Peptide
R.HAVINDLPLGR.N
R.GSFLLDKDGTVR.H
R.GFDVLLEDAGVALR.G
gi|157164012
Mass: 22011
Score: 154
Queries matched: 3
gi|255321903
Mass: 21900
Score: 154
Queries matched: 3
2-Cys peroxiredoxin BAS1 [Campylobacter showae RM3277]
38.
gi|157163930
Mass: 45716
Score: 153
Queries matched: 4
outer membrane protein [Campylobacter concisus 13826]
emPAI: 0.07
Query
155
333
393
394
39.
Observed
489.7536
606.7801
635.8447
635.8448
Mr(expt)
977.4926
1211.5456
1269.6748
1269.6750
Mr(calc)
977.4930
1211.5458
1269.6757
1269.6757
-0.40
0
46
0.046
1
-0.16
0
38
0.13
1
-0.70
0
69 0.00013
1
-0.54
0
(58) 0.0014
1
gi|157164579
Mass: 31381
Score: 148
Queries matched: 4
Peptide
K.NVDLSGFAR.Y
R.NEWSLDASYK.Y
K.LTFLSWYAAAK.D
K.LTFLSWYAAAK.D
emPAI: 0.11
Query
120
288
342
792
40.
Observed
469.2622
573.2808
615.8276
1035.0530
Mr(expt)
936.5098
1144.5470
1229.6406
2068.0914
Mr(calc)
936.5103
1144.5472
1229.6404
2068.0953
-0.43
0
27
1.3
1
-0.17
0
69 0.0001
1
0.18
0
26
4.1
1
-1.86
0
26
1.8
1
gi|157164268
Mass: 36935
Score: 147
Queries matched: 3
ketol-acid reductoisomerase [Campylobacter concisus 13826]
Peptide
R.IAFNMITK.M
K.VSNDDALNAAR.A
K.VLFIAPDNGER.Y
K.IIAVEPEASPVLSGGNPGPHK.I
emPAI: 0.29
Query
445
594
727
41.
Observed
676.8839
813.4290
940.9365
Mr(expt)
1351.7532
1624.8434
1879.8584
Mr(calc)
1351.7533
1624.8420
1879.8588
-0.06
0
52 0.0032
1
0.87
0
39
0.11
1
-0.19
0
55 0.0026
1
Peptide
K.APANIDVIMIAPK.A
R.GGGIPDLIAVEQNASGK.A
R.YSISNTAEYGDYVSGVR.V
gi|157165110
Mass: 29741
Score: 144
Queries matched: 4
emPAI: 0.24
hydrogenase accessory protein HypB [Campylobacter concisus 13826]
Query
128
170
542
661
42.
Observed
472.2955
495.2976
759.3723
865.9129
Mr(expt)
942.5764
988.5806
1516.7300
1729.8112
Mr(calc)
942.5750
988.5804
1516.7310
1729.8119
1.57
0
48 0.0097
1
0.21
0
29
0.6
1
-0.65
0
26
3
1
-0.36
0
42
0.06
1
Peptide
R.AADVLLITK.A
K.TTLLEATIK.A
K.ASLAPHFDFDIER.V
K.IGVVEGDLETNQDADR.I
gi|6320157
Mass: 19051
Score: 137
Queries matched: 6
emPAI: 0.63
Query
346
347
367
451
736
737
Observed
617.8553
617.8556
625.3301
681.9030
961.5582
641.3747
Mr(expt)
1233.6960
1233.6966
1248.6456
1361.7914
1921.1018
1921.1023
Mr(calc)
1233.6969
1233.6969
1248.6462
1361.7918
1921.1037
1921.1037
-0.68
0
54 0.0019
1
-0.19
0
(48) 0.0097
1
-0.47
0
29
1.5
1
-0.29
1
24
1.1
1
-0.96
1
30
0.14
1
-0.73
1
(27)
0.28
1
Peptide
K.IVEIPGEVPPGK.Y
K.IVEIPGEVPPGK.Y
K.EVNLDPNPPVR.G
K.KIVEIPGEVPPGK.Y
gi|151941952
Mass: 19021
Score: 137
Queries matched: 6
12/9/2009
Page 12 of 22
gi|190405057
Mass: 19007
Score: 137
Queries matched: 6
43.
gi|157164144
Mass: 33246
Score: 136
Queries matched: 4
thioredoxin-disulfide reductase [Campylobacter concisus 13826]
emPAI: 0.21
44.
Query
521
522
794
Observed
496.5996
744.3965
1039.4870
Mr(expt)
1486.7770
1486.7784
2076.9594
Mr(calc)
1486.7780
1486.7780
2076.9600
ppm Miss Score
Expect Rank
-0.68
1
28
2.4
1
0.31
1
(27)
2.3
1
-0.25
0
71 7.1e-005
1
Peptide
K.GLFVAGDIREDAPK.Q
K.GLFVAGDIREDAPK.Q
K.GEPGGQITSSSEIENYPGQK.A
845
791.7416
2372.2030
2372.2046
-0.68
K.QVIVAAGDGAVAALSAMSYIESLH.-
0
37
0.19
gi|157164859
Mass: 27476
Score: 122
Queries matched: 5
1
emPAI: 0.12
Query
324
384
583
654
662
45.
Observed
601.3244
632.8299
803.3979
860.4537
868.4500
Mr(expt)
1200.6342
1263.6452
1604.7812
1718.8928
1734.8854
Mr(calc)
1200.6350
1263.6459
1604.7794
1718.8913
1734.8862
-0.61
0
29
1.5
1
-0.54
0
30
1.5
1
1.12
0
35
0.4
1
0.92
0
(24)
4.7
1
-0.42
0
28
1.8
1
Peptide
K.GNTELLEAINK.A
K.NGATVVVADGFSK.G
K.ADVVFNQVSINEDR.K
gi|157165776
Mass: 34805
Score: 118
Queries matched: 4
putative carbon storage regulator-like protein [Campylobacter concisus 13826]
Query
157
361
378
438
46.
Observed
490.2583
623.8631
629.8612
670.3352
Mr(expt)
978.5020
1245.7116
1257.7078
1338.6558
Mr(calc)
978.5022
1245.7122
1257.7081
1338.6568
-0.12
0
35
0.56
1
-0.41
0
40
0.077
1
-0.19
1
23
5.7
1
-0.71
0
21
8.7
1
Peptide
K.LYEENLAK.F
K.VIFVAPQFPTK.A
K.ALIEKFPQNAK.L
K.GADPHTYEPKPK.Q
gi|158604943
Mass: 37938
Score: 107
Queries matched: 3
emPAI: 0.09
aspartate-semialdehyde dehydrogenase [Campylobacter concisus 13826]
Query
433
696
786
47.
Observed
662.3849
602.9639
1022.5370
Mr(expt)
1322.7552
1805.8699
2043.0594
Mr(calc)
1322.7558
1805.8697
2043.0598
-0.42
1
51 0.0042
1
0.11
0
24
5.8
1
-0.18
0
32
0.54
1
Peptide
K.APSVVVVDNPAKK.E
K.FAADSGAVVIDNTSHFR.M
R.VMEEVDFPVGELLPLASAK.S
gi|157164277
Mass: 36126
Score: 106
Queries matched: 3
emPAI: 0.19
glyceraldehyde-3-phosphate dehydrogenase, type I [Campylobacter concisus 13826]
Query
446
504
609
Observed
678.8775
732.3906
828.4736
Mr(expt)
1355.7404
1462.7666
1654.9326
Mr(calc)
1355.7409
1462.7668
1654.9328
-0.30
0
50 0.0083
1
-0.07
1
22
6.6
1
-0.08
0
34
0.13
1
Peptide
R.AAALNIGPTTTGAAK.A
K.VISDDFIEVNGKK.I
R.VPVANVSMVDLTAVLK.R
gi|255323513
Mass: 36026
Score: 106
Queries matched: 3
glyceraldehyde-3-phosphate dehydrogenase, type I [Campylobacter showae RM3277]
48.
gi|157164748
Mass: 39704
Score: 105
Queries matched: 2
emPAI: 0.17
methyl-accepting chemotaxis protein [Campylobacter concisus 13826]
49.
Query
232
Observed
531.7820
Mr(expt)
1061.5494
Mr(calc)
1061.5506
-1.06
0
50
0.012
1
Peptide
R.GFAVVADEVR.K
329
603.3240
1204.6334
1204.6339
-0.40
K.AGFEELLEAVK.A
0
55
0.0045
gi|157165527
Mass: 43876
Score: 100
Queries matched: 3
hydrogenase-4 component I [Campylobacter concisus 13826]
1
emPAI: 0.08
Query
117
155
595
50.
Observed
467.2266
489.7536
816.3726
gi|157165509
Mr(expt)
932.4386
977.4926
1630.7306
Mass: 37709
Mr(calc)
932.4392
977.4930
1630.7304
-0.59
0
27
2.4
1
-0.40
0
46
0.046
1
0.17
0
27
1.2
1
Score: 84
Queries matched: 2
Peptide
R.FAYQYNK.K
K.NVDLSGFAR.Y
K.GNFFYATSAYTFDK.F
emPAI: 0.18
12/9/2009
Page 13 of 22
DNA-directed RNA polymerase subunit alpha [Campylobacter concisus 13826]
Query
470
555
51.
Observed
708.8886
783.4500
Mr(expt)
1415.7626
1564.8854
Mr(calc)
1415.7620
1564.8865
0.47
0
40
0.08
1
-0.67
0
43
0.03
1
Peptide
K.LLSSVEDLNLSAR.S
R.LLYTSTVGFAPIGVK.I
gi|119916467
Mass: 44475
Score: 79
Queries matched: 2
emPAI: 0.07
PREDICTED: similar to serpin peptidase inhibitor, clade B like [Bos taurus]
Query
284
434
Observed
568.3033
663.3201
Mr(expt)
1134.5920
1324.6256
Mr(calc)
1134.5921
1324.6259
-0.03
0
26
3.5
1
-0.18
0
54 0.0039
1
Peptide
K.FVSLEDVQAK.I
K.STDAYELSVANR.L
gi|119916469
Mass: 44234
Score: 79
Queries matched: 2
gi|119916473
Mass: 44489
Score: 79
Queries matched: 2
gi|119923092
Mass: 44484
Score: 79
Queries matched: 2
gi|119923094
Mass: 44261
Score: 79
Queries matched: 2
gi|119923096
Mass: 44332
Score: 79
Queries matched: 2
Mass: 44370
Score: 79
Queries matched: 2
gi|119924105
PREDICTED: serine (or cysteine) proteinase inhibitor, clade B [Bos taurus]
gi|156121289
Mass: 38623
Score: 79
Queries matched: 2
gi|157785603
Mass: 44029
Score: 79
Queries matched: 2
serpin peptidase inhibitor, clade B like [Bos taurus]
52.
gi|66046197
Mass: 68703
Score: 79
Queries matched: 2
emPAI: 0.05
histidine kinase, HAMP region: chemotaxis sensory transducer [Pseudomonas syringae pv. syringae B728a]
Query
18
232
Observed
373.2136
531.7820
Mr(expt)
744.4126
1061.5494
Mr(calc)
744.4130
1061.5506
-0.44
0
29
5.7
1
-1.06
0
50
0.012
1
Peptide
K.ADALSLR.I
R.GFAVVADEVR.S
gi|71738069
Mass: 68791
Score: 79
Queries matched: 2
methyl-accepting chemotaxis protein [Pseudomonas syringae pv. phaseolicola 1448A]
53.
gi|181402
Mass: 65825
Score: 79
Queries matched: 2
Query
150
435
Observed
487.2686
665.3665
Mr(expt)
972.5226
1328.7184
Mr(calc)
972.5240
1328.7187
-1.36
0
32
1.3
2
-0.20
0
47
0.022
1
Peptide
K.IEISELNR.V
R.NLDLDSIIAEVK.A
Mass: 65393
Score: 79
Queries matched: 2
gi|47132620
gi|148727309
Mass: 65944
Score: 79
Queries matched: 2
54.
gi|157163909
Mass: 45295
Score: 78
Queries matched: 1
serine hydroxymethyltransferase [Campylobacter concisus 13826]
emPAI: 0.07
Query
660
55.
Observed
865.4520
Mr(expt)
1728.8894
Mr(calc)
1728.8894
0.05
0
78 2e-005
1
Peptide
K.IADVLSDINNASLQEK.I
gi|157164709
Mass: 47282
Score: 76
Queries matched: 3
emPAI: 0.07
peptidoglycan associated lipoprotein [Campylobacter concisus 13826]
Query
206
207
655
56.
Observed
514.3287
514.3293
863.4725
gi|225632
Mr(expt)
1026.6428
1026.6440
1724.9304
Mass: 24420
Mr(calc)
1026.6437
1026.6437
1724.9309
Score: 74
-0.85
0
55 0.0011
1
0.32
0
(47) 0.0075
1
-0.24
0
21
6.6
1
Queries matched: 4
Peptide
K.TPITITILR.K
K.TPITITILR.K
K.GSVQVILPIDDTEALR.L
emPAI: 0.29
12/9/2009
Page 14 of 22
casein alphaS1
Query
390
391
461
462
Observed
634.3557
634.3557
692.8685
692.8686
Mr(expt)
1266.6968
1266.6968
1383.7224
1383.7226
Mr(calc)
1266.6972
1266.6972
1383.7227
1383.7227
-0.27
0
39
0.093
1
-0.27
0
(23)
3.6
1
-0.21
0
(25)
3.1
1
-0.06
0
35
0.3
1
Peptide
R.YLGYLEQLLR.L
R.YLGYLEQLLR.L
R.FFVAPFPEVFGK.E
R.FFVAPFPEVFGK.E
Mass: 24513
Score: 74
Queries matched: 4
gi|30794348
gi|70905149
Mass: 24383
Score: 74
Queries matched: 4
alpha-S1 casein [Bubalus bubalis]
Mass: 24311
Score: 74
Queries matched: 4
gi|75038951
RecName: Full=Alpha-S1-casein; Flags: Precursor
gi|159793187
Mass: 13839
Score: 74
Queries matched: 4
gi|159793191
Mass: 23598
Score: 74
Queries matched: 4
gi|159793193
Mass: 18671
Score: 74
Queries matched: 4
gi|159793197
Mass: 23473
Score: 74
Queries matched: 4
gi|159793209
Mass: 22442
Score: 74
Queries matched: 4
gi|195973860
Mass: 24427
Score: 74
Queries matched: 4
57.
gi|114667176
Mass: 107386
Score: 72
Queries matched: 2
emPAI: 0.03
Query
40
296
58.
Observed
404.2031
579.2988
Mr(expt)
806.3916
1156.5830
Mr(calc)
806.3923
1156.5836
-0.76
0
24
4.3
1
-0.52
0
48
0.014
1
gi|27806941
Mass: 46075
Score: 70
Queries matched: 2
serine proteinase inhibitor, clade A, member 1 [Bos taurus]
Peptide
R.LAADDFR.T
R.QGVDADINGLR.Q
emPAI: 0.07
Query
304
745
59.
Observed
584.8087
647.3560
Mr(expt)
1167.6028
1939.0462
Mr(calc)
1167.6023
1939.0455
0.47
0
36
0.23
1
0.35
0
34
0.24
1
Peptide
K.LSISETYDLK.S
K.VLDPNTVFALVNYISFK.G
gi|157164923
Mass: 39225
Score: 69
Queries matched: 2
peptide methionine sulfoxide reductase family protein [Campylobacter concisus 13826]
Query
91
449
60.
Observed
439.7523
679.3557
Mr(expt)
877.4900
1356.6968
Mr(calc)
877.4909
1356.6965
-1.01
0
30
1.2
1
0.23
0
39
0.14
1
gi|157164833
Mass: 45147
Score: 59
Queries matched: 2
phosphopyruvate hydratase [Campylobacter concisus 13826]
Peptide
K.IFGVVDTK.V
R.LGYEEFIPYVK.-
emPAI: 0.07
Query
153
563
61.
Observed
488.2848
788.9147
Mr(expt)
974.5550
1575.8148
Mr(calc)
974.5549
1575.8145
0.17
0
21
14
2
0.25
0
38
0.18
1
Peptide
K.SLNIPLYR.Y
K.VQLVGDDLFVTNEK.I
gi|154174678
Mass: 36635
Score: 57
Queries matched: 2
outer membrane fibronectin-binding protein [Campylobacter curvus 525.92]
Query
108
144
62.
Observed
453.2633
482.7640
Mr(expt)
904.5120
963.5134
Mr(calc)
904.5130
963.5138
-1.07
1
26
2.5
1
-0.33
1
31
1.2
1
gi|157164023
Mass: 34756
Score: 50
Queries matched: 1
carbon-nitrogen family hydrolase [Campylobacter concisus 13826]
Peptide
R.FALKAEAR.D
R.RVEATFNK.-
emPAI: 0.10
Query
432
Observed
661.8635
Mr(expt)
1321.7124
Mr(calc)
1321.7129
-0.35
0
50 0.0074
1
Peptide
K.ILILSEGDYATK.V
12/9/2009
Page 15 of 22
Query
490
159
253
484
656
115
539
703
210
805
567
202
702
586
49
138
306
109
162
150
309
29
455
355
123
142
90
77
34
122
87
177
557
227
73
305
319
469
185
321
556
111
36
61
180
70
350
Observed
717.8646
490.7511
550.3214
713.3801
863.9707
465.7719
505.9106
914.4413
516.2997
1071.9970
793.9307
511.2631
914.4408
807.4359
415.2483
480.2919
584.8506
454.7452
492.3032
487.2686
585.8579
395.2395
685.8588
622.8457
470.2969
481.7396
437.7536
432.7446
398.7137
469.2713
435.7738
498.2635
783.8739
529.2800
429.7496
584.8500
590.8525
708.3690
503.7635
596.3173
783.8739
458.2559
403.2442
422.7372
499.8029
428.7660
619.8299
Mr(expt)
1433.7146
979.4876
1098.6282
1424.7456
1725.9268
929.5292
1514.7100
1826.8680
1030.5848
2141.9794
1585.8468
1020.5116
1826.8670
1612.8572
828.4820
958.5692
1167.6866
907.4758
982.5918
972.5226
1169.7012
788.4644
1369.7030
1243.6768
938.5792
961.4646
873.4926
863.4746
795.4128
936.5280
869.5330
994.5124
1565.7332
1056.5454
857.4846
1167.6854
1179.6904
1414.7234
1005.5124
1190.6200
1565.7332
914.4972
804.4738
843.4598
997.5912
855.5174
1237.6452
Mr(calc)
1433.7150
979.4876
1098.6285
1424.7452
1725.9261
929.5294
1514.7113
1826.8686
1030.5845
2141.9793
1585.8464
1020.5128
1826.8686
1612.8573
828.4818
958.5699
1167.6863
907.4763
982.5923
972.5240
1169.7020
788.4644
1369.7030
1243.6772
938.5800
961.4651
873.4960
863.4752
795.4126
936.5280
869.5334
994.5157
1565.7283
1056.5465
857.4858
1167.6863
1179.6903
1414.7238
1005.5131
1190.6217
1565.7395
914.4974
804.4745
843.4603
997.5920
855.5178
1237.6489
ppm Miss Score
-0.27
0
48
0.07
0
48
-0.21
0
48
0.31
0
46
0.43
0
46
-0.17
0
46
-0.91
0
45
-0.32
0
45
0.35
0
44
0.06
0
43
0.29
0
42
-1.09
0
41
-0.87
0
40
-0.02
0
40
0.36
1
40
-0.66
0
39
0.29
0
39
-0.50
1
39
-0.49
0
37
-1.36
0
37
-0.61
0
36
0.10
0
36
0.01
0
36
-0.28
0
35
-0.85
0
35
-0.46
1
34
-3.82
0
34
-0.70
0
33
0.26
0
33
0.04
1
32
-0.46
0
32
-3.32
0
32
3.16
1
32
-0.97
1
32
-1.39
0
32
-0.74
0
32
0.09
0
31
-0.26
0
31
-0.64
1
31
-1.35
0
30
-4.00
0
30
-0.16
1
30
-0.85
1
30
-0.50
0
29
-0.79
0
29
-0.42
0
29
-2.93
0
29
Expect Rank
0.022
1
0.018
1
0.013
1
0.022
1
0.017
1
0.044
1
0.027
1
0.039
1
0.046
1
0.038
1
0.054
1
0.088
1
0.11
1
0.092
1
0.24
1
0.063
1
0.047
1
0.2
1
0.068
1
0.44
1
0.076
1
0.16
1
0.29
1
0.29
1
0.056
1
0.52
1
1
1
0.77
1
0.39
1
0.54
1
0.79
1
0.44
1
0.58
1
0.52
1
1
1
0.21
1
0.18
1
0.81
1
1.4
1
0.96
1
0.9
1
0.92
1
0.59
1
0.57
1
0.85
1
1.6
1
1.6
1
Peptide
ALDAPSGVYNAATGK
VGYVSGWGR
VAGDIEAVVVK
AIQAAFFYLEPR
LLPTNAVEILQTSGDR
SSNLLQLR
VGAHAGDYGAEALER
GEADALNLDGGYIYTAGK
LQCNTIVLK
YSTTPAQLDSDGSYFLYSK
AIAPTFGGINLEDIR
EFLIEDAGK
GEADALNLDGGYIYTAGK
NLQFVELEGAQIPR
VASIRVGQ
VVATLSLEK
LIDVLLEPTR
SEKEFIR
ALGILNLNR
NGAISEIAAK
ELIGVTQGLIK
IVTITDK
TNYGVAFFPNLK
AGSGEVGINLLSK
IAVEVAPLK
NVDEAMKR
EAVLPFAK
YIPGISSK
ASSAYLGK
ADKTYVIK
VVSLSIPR
VFDCLSLK + Acrylamide (C)
WVGRTPADR
VLEVVDGK
LIDVLLEPTR
VLIVVSPYYK
ACENAAVLGAIAGAK + Carbamidomethyl (C)
YPQIDKDK
NEMSNLNDIFIEK
AGDFHKLK
VFKALDL
NWLNAVK
LIGPTSVVGR
LATVVTPR
SMQLTNILFR + Oxidation (M)
167
10
334
228
89
815
398
121
260
328
454
494.2665
359.2288
607.8008
529.2802
437.7167
1104.5320
637.8597
469.2710
554.7852
603.3171
684.8779
986.5184
716.4430
1213.5870
1056.5458
873.4188
2207.0494
1273.7048
936.5274
1107.5558
1204.6196
1367.7412
986.5219
716.4432
1213.5867
1056.5498
873.4192
2207.0481
1273.7064
936.5280
1107.5560
1204.6200
1367.7409
-3.48
-0.24
0.30
-3.77
-0.39
0.60
-1.21
-0.61
-0.16
-0.31
0.28
0
0
0
1
0
1
1
1
0
1
0
29
28
28
28
28
28
27
27
26
26
26
1.8
2.6
1.4
1.6
1.9
1.7
1.8
1.8
3.4
3.4
1.7
1
1
1
1
1
1
1
1
1
1
1
CTLPNLQK + Acrylamide (C)
AVIISSK
DFTYVDDIVK
CASVPRTAPR
VANEGEQK
LLDKIEDDDDVQAVYTNIE
KEGVGLGLSICK + Acrylamide (C)
ADKTYVIK
VGYISTSPER
SKFDPNLTQR
ATNVLLDGNLNPK
103
471
152
201
242
156
308
603
505
266
587
230
282
52
450.7556
709.4121
488.2768
510.7714
542.2749
489.7536
585.3118
548.3047
488.9206
556.8027
807.8478
531.2740
566.7867
417.2395
899.4966
1416.8096
974.5390
1019.5282
1082.5352
977.4926
1168.6090
1641.8923
1463.7400
1111.5908
1613.6810
1060.5334
1131.5588
832.4644
899.4964
1416.8089
974.5396
1019.5321
1082.5356
977.4964
1168.6088
1641.8937
1463.7409
1111.5913
1613.6856
1060.5335
1131.5632
832.4654
0.28
0.54
-0.61
-3.78
-0.37
-3.82
0.22
-0.88
-0.62
-0.44
-2.84
-0.06
-3.89
-1.17
0
0
1
1
0
1
1
0
0
1
0
1
1
0
26
26
26
26
25
25
25
25
25
25
25
25
25
24
3
0.93
2.4
5.2
1.6
5.4
3
2.4
5.2
2.7
0.98
6.1
4
3.6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
TPIDDVIK
ILALINSFSQGVR
VANTDLKSK
SIEMKVEGK
DSAGVAPDPVR
MNSANVKSK
LADSVRDSLPP
NINEVIEGVISTLNK
AVSVFTWAAEEVR
YLKSTFPEK
MACGVGACLVCTCK + 2 Acrylamide (C); 2 Carbamidomethyl (C)
MASIKGPGER + Oxidation (M)
VTTNDQGNKR
SIGSQTIK
12/9/2009
Page 16 of 22
302
732
430
605
194
21
401
153
506
221
585
582.7858
949.5225
658.8745
548.3054
507.8135
382.2265
639.3037
488.2848
732.8777
525.7692
807.4199
1163.5570
1897.0304
1315.7344
1641.8944
1013.6124
762.4384
1276.5928
974.5550
1463.7408
1049.5238
1612.8252
1163.5531
1897.0309
1315.7347
1641.8937
1013.6121
762.4388
1276.5904
974.5549
1463.7442
1049.5249
1612.8243
3.42
-0.22
-0.20
0.40
0.37
-0.46
1.93
0.17
-2.31
-1.00
0.59
1
0
0
0
0
1
0
1
1
0
0
24
24
24
24
24
24
24
24
23
23
23
4.7
2.3
2.8
2.5
2.2
8.5
3.9
7.1
6.9
4.5
5.1
1
1
1
1
1
1
1
1
1
1
1
DTKSTNQQSR
AYNTQILAEIPIEPAVR
TGTLDLSANLALK
NINEVIEGVISTLNK
IATQEVLIK
IREFAK
SLNIWSKK
MRLSTWGIEIDK + Oxidation (M)
MIECIINK + Acrylamide (C); Oxidation (M)
MLGEVNTQPQVVGNK
80
145
71
520
163
22
102
215
564
41
93
68
74
332
433.7113
484.2494
428.7661
495.6001
493.7486
384.2244
450.7266
517.7689
791.3912
404.7317
441.7393
426.7505
429.7508
606.3659
865.4080
966.4842
855.5176
1483.7785
985.4826
766.4342
899.4386
1033.5232
1580.7678
807.4488
881.4640
851.4864
857.4870
1210.7172
865.4083
966.4804
855.5178
1483.7745
985.4804
766.4337
899.4357
1033.5226
1580.7617
807.4490
881.4607
851.4865
857.4858
1210.7173
-0.25
3.96
-0.15
2.69
2.32
0.69
3.28
0.63
3.89
-0.23
3.84
-0.04
1.41
-0.02
0
0
0
0
0
0
1
0
1
1
1
0
0
0
23
23
23
23
23
23
23
23
22
22
22
22
22
22
1.5
4.5
6.4
4.9
2.7
4.5
3.8
11
8
8.1
5.2
2.9
8
2.5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
GVFSWDR
MTASSVLSR + Oxidation (M)
IAISLPSR
NSLFLTFCELIK + Carbamidomethyl (C)
GHLTYHMK
QHIIEK
YLSCMRK
LQEIASMAR + Oxidation (M)
GGKMEDVIFGGTQAR + Oxidation (M)
ISKGEFK
TRYVSEK
LAPADLPR
VLEVVDGK
ELLTVISPLNI
229
98
63
235
482
560
570
341
60
343
104
743
169
281
200
770
183
190
62
240
307
81
761
132
679
38
326
495
529.3093
443.7522
424.7305
537.8005
475.5716
525.2628
798.9510
614.8337
421.7583
411.8991
450.7558
645.6782
494.7870
565.7700
510.2689
497.7671
502.2330
506.2626
422.7375
541.2852
585.3116
433.7415
983.5134
476.2517
887.9519
403.7114
602.3143
721.4102
1056.6040
885.4898
847.4464
1073.5864
1423.6930
1572.7666
1595.8874
1227.6528
841.5020
1232.6755
899.4970
1934.0128
987.5594
1129.5254
1018.5232
1987.0393
1002.4514
1010.5106
843.4604
1080.5558
1168.6086
865.4684
1965.0122
950.4888
1773.8892
805.4082
1202.6140
1440.8058
1056.6040
885.4920
847.4473
1073.5903
1423.6943
1572.7606
1595.8883
1227.6499
841.5021
1232.6799
899.4937
1934.0109
987.5600
1129.5291
1018.5270
1987.0417
1002.4480
1010.5107
843.4603
1080.5539
1168.6088
865.4698
1965.0128
950.4895
1773.8897
805.4083
1202.6143
1440.8048
0.09
-2.40
-1.04
-3.59
-0.95
3.80
-0.51
2.36
-0.10
-3.56
3.72
0.98
-0.60
-3.26
-3.65
-1.20
3.40
-0.00
0.20
1.84
-0.13
-1.55
-0.30
-0.72
-0.26
-0.04
-0.19
0.71
1
0
0
0
1
0
1
0
0
1
1
1
0
0
1
1
0
0
0
0
1
0
0
0
0
0
0
1
22
22
22
22
22
22
21
21
21
21
21
21
21
21
21
20
20
20
20
20
20
20
20
20
20
20
20
20
7.2
12
13
13
8.6
8.9
4.4
7.2
11
8.2
9.7
6.9
7.7
6.1
16
7.7
5.7
11
8.7
6.8
8
12
9.5
8.4
16
9.4
11
6.7
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
SGNRLELLR
LGPETTLR
MLATTSPK
LQMEVVALR + Oxidation (M)
LNYGGSDKGDAVTK
ISMESIYHHSELK
QDSVVIKGIDNAIPK
FPVADVEGGLPK
LASVSPLR
IKIGDNVTVMK + Oxidation (M)
EAGNVVRR
QQYGEGIATAVKQTLEAK
ELQSTLAVK
YYVEEVSNK
EMFAIHKK + Oxidation (M)
ILTALCLCPAGRDLCVK + 2 Acrylamide (C); Carbamidomethyl (C)
SNEYFCIK
LMDIVYNK + Oxidation (M)
YQIVGHK
MPFPLPHAR + Oxidation (M)
LADSVRDSLPP
SFQFPLK
IDMLYAIIASDVANSLEK
TMSQPFLK
INTVQEAIDFVAANAAQ
FVGDGGVR
NTDGSITSLPAK
LANRINELLNSGK
388
450
27
269
497
223
695
133
237
710
414
84
437
37
422.8751
679.3588
385.7078
557.8452
723.4113
526.2639
600.6623
476.2603
537.8114
616.6548
433.9270
434.2559
669.3458
403.7111
1265.6035
1356.7030
769.4010
1113.6758
1444.8080
1050.5132
1798.9651
950.5060
1073.6082
1846.9426
1298.7592
866.4972
1336.6770
805.4076
1265.6040
1356.7038
769.4010
1113.6757
1444.8038
1050.5134
1798.9651
950.5073
1073.6094
1846.9425
1298.7558
866.4974
1336.6809
805.4083
-0.44
-0.54
0.04
0.10
2.92
-0.19
-0.03
-1.28
-1.08
0.06
2.63
-0.15
-2.89
-0.78
0
1
0
1
0
0
0
0
1
1
1
0
0
0
20
20
20
20
19
19
19
19
19
19
19
19
19
19
14
14
5.9
4.7
7.9
16
7.6
14
10
15
6.3
6.7
16
11
1
1
1
1
1
1
1
1
1
1
1
1
1
1
ALETHTYFER
GVTFTDRGTYIK
AEFYIK
DILSQLGIKK
ANGGVFLGLNGTVVK
LFDHYQTK
EAINTYLK
RAVPPGPGPAR
GITIATSHIEYETEKR
KNLIQNILESK
EHIILSR
IAAMGYDVTQLR
FVGDGGVR
614
222
267
30
396
537
75
126
528
559.2686
526.2636
557.8448
395.7139
424.5761
504.9226
430.2119
471.7844
747.3904
1674.7840
1050.5126
1113.6750
789.4132
1270.7065
1511.7460
858.4092
941.5542
1492.7662
1674.7850
1050.5128
1113.6757
789.4133
1270.7067
1511.7468
858.4083
941.5546
1492.7681
-0.59
-0.13
-0.62
-0.08
-0.21
-0.51
1.09
-0.35
-1.24
0
0
1
1
0
1
0
0
1
19
19
19
19
19
19
19
19
19
12
18
5
8
9.3
17
7.2
12
16
1
1
1
1
1
1
1
1
1
GSYDGVDHDVNTIGVK
DILSGKILAGK
KNNWTK
SDKPVVLVGAMR
DSGLTSDLSSFAGKK
IDTDGNPK
TIGNTPIVK
LGCVRHGAAQSPAR + Acrylamide (C)
12/9/2009
Page 17 of 22
161
130
356
832
859
738
839
873
244
352
364
491.2841
475.7143
622.8659
761.7316
846.0683
641.3751
767.0634
902.1259
543.7825
620.8376
416.5991
980.5536
949.4140
1243.7172
2282.1730
2535.1831
1921.1035
2298.1684
2703.3559
1085.5504
1239.6606
1246.7755
980.5542
949.4175
1243.7176
2282.1729
2535.1833
1921.1037
2298.1678
2703.3537
1085.5505
1239.6612
1246.7761
-0.61
-3.61
-0.29
0.04
-0.09
-0.11
0.25
0.79
-0.08
-0.42
-0.52
0
0
0
0
0
1
0
1
1
1
0
19
19
19
19
19
19
18
18
18
18
18
7.4
11
8.7
14
14
2.3
13
17
16
13
0.57
1
1
1
1
1
1
1
1
1
1
1
YLTTGSVIK
NEADQMVK + Oxidation (M)
LATLIPLFDNK
TTDVTGSITLPEGTEMVMPGDNVR + Oxidation (M)
IVEIPGEVPPGKYVVVAR
IATIKGEQISNIGSQDMNNEVWLK + Oxidation (M)
GYTEGYLRK
YQVTDRFALK
VLHLLGGANLLK
26
837
377
602
402
82
283
878
565
850
689
417
279
312
385.1990
764.7260
629.3656
821.4366
641.3090
433.7420
566.8399
940.8434
791.3917
811.6996
895.4700
433.9272
376.5451
586.3394
768.3834
2291.1562
1256.7166
1640.8586
1280.6034
865.4694
1131.6652
2819.5084
1580.7688
2432.0770
1788.9254
1298.7598
1126.6135
1170.6642
768.3840
2291.1533
1256.7169
1640.8603
1280.6037
865.4698
1131.6652
2819.5103
1580.7716
2432.0781
1788.9266
1298.7558
1126.6135
1170.6648
-0.72
1.27
-0.19
-0.99
-0.21
-0.39
0.06
-0.69
-1.72
-0.48
-0.65
3.09
-0.02
-0.51
0
1
0
1
0
0
0
1
1
0
1
1
0
0
18
18
18
18
18
18
18
18
18
18
18
18
18
18
14
16
8.3
18
42
2.8
8.1
6.6
17
8.7
20
9
16
18
1
1
1
1
1
1
1
1
1
1
1
1
1
1
MIFTNK + Oxidation (M)
VFSDEADLSGVTEEQPLKLSK
VVLVNYIYFK
FFVAPFPEVFGKEK
SDLYVSDAFHK
FSWLVSK
LHPLPISDLK
VGDTIEIVGIKPTQTTTVTGVEMFRK
NTIMASKSVGTAEEK + Oxidation (M)
TPFNPNHTYESEFHVSQNER
VMNNLNLPYPKMIDK
LINAGKILENSK
FAHVVAASTPK
AYPTTPALIPK
106
79
562
796
840
94
6
715
178
95
519
527
158
344
669
638
86
165
357
47
113
729
589
2
647
719
404
636
451.2663
433.2481
788.4299
696.0241
1150.0920
441.7395
356.2128
620.2988
498.2647
442.2190
494.5880
747.3716
490.2838
617.3456
587.3206
568.2744
435.7735
493.7793
622.8660
411.7394
459.7661
947.9402
808.9357
350.2110
571.9697
620.3138
641.3094
566.6389
900.5180
864.4816
1574.8452
2085.0505
2298.1694
881.4644
710.4110
1857.8746
994.5148
882.4234
1480.7422
1492.7286
978.5530
1232.6766
1758.9400
1701.8014
869.5324
985.5440
1243.7174
821.4642
917.5176
1893.8658
1615.8568
698.4074
1712.8873
1857.9196
1280.6042
1696.8949
900.5215
864.4817
1574.8450
2085.0490
2298.1678
881.4647
710.4115
1857.8745
994.5157
882.4269
1480.7456
1492.7270
978.5538
1232.6798
1758.9338
1701.7992
869.5334
985.5444
1243.7176
821.4647
917.5182
1893.8666
1615.8570
698.4075
1712.8886
1857.9221
1280.6037
1696.8971
-3.83
-0.10
0.16
0.69
0.72
-0.31
-0.68
0.05
-0.89
-3.94
-2.33
1.12
-0.82
-2.59
3.50
1.28
-1.15
-0.36
-0.13
-0.54
-0.57
-0.39
-0.07
-0.08
-0.78
-1.35
0.42
-1.29
0
1
1
1
0
0
0
0
0
0
1
1
0
1
0
0
0
0
0
0
1
0
0
1
0
0
0
1
18
18
18
18
18
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
16
12
18
18
17
6.2
4.2
68
12
20
23
27
18
22
16
19
25
22
12
23
19
15
28
27
21
25
54
19
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
LLCIGLGGR
FRIQSSK
CQSATLNLVNSLKK + Carbamidomethyl (C)
AYLQGQIGNPEGEDKPNKK
QVVDFFK
YFIIR
LSIYGNDYDTIDGTGVR
MIYDNIVK
SCNTIFK + Acrylamide (C)
SSGLIGYCVAERR + Acrylamide (C)
SQYEQLAEQNRK
FAISVPFAK
SKDICVLLQSK
IPDFTLLAEALGCAGLR
EMDQGEAGDNVGVLLR
VVSLSIPR
IVDVGIENK
LATLIPLFDNK
TVAAYLGK
EAKTLTAGK
EQLQDMGLEDLFSPEK + Oxidation (M)
FGVSSAAGIGGLPNIEK
ADRLPK
LSGFDVNALYPHGVPK
VVLAGHTDSVGAEAYNQK
SDLYVSDAFHK
LSSTCRVYLPFIGGK + Carbamidomethyl (C)
525
529
720
154
685
313
553
4
14
834
43
533
300
105
745.3860
747.4128
620.6666
489.7373
892.4920
586.3397
782.8805
350.7336
363.6946
761.7318
408.2183
752.8672
581.8101
451.2661
1488.7574
1492.8110
1858.9780
977.4600
1782.9694
1170.6648
1563.7464
699.4526
725.3746
2282.1736
814.4220
1503.7198
1161.6056
900.5176
1488.7572
1492.8112
1858.9797
977.4600
1782.9702
1170.6608
1563.7459
699.4531
725.3748
2282.1729
814.4225
1503.7214
1161.6064
900.5181
0.14
-0.11
-0.95
0.06
-0.43
3.46
0.36
-0.59
-0.25
0.30
-0.54
-1.02
-0.61
-0.53
1
0
1
1
0
1
0
0
0
0
1
0
1
0
17
17
17
17
17
16
16
16
16
16
16
16
16
16
28
15
23
26
15
24
22
12
3.8
23
14
31
31
48
1
1
1
1
1
1
1
1
1
1
1
1
1
1
ERSVEDWTNLLK
QVGVPYIVVFMNK
CLHLILTMSPIGDKFR + Oxidation (M)
KAEQEMAR + Oxidation (M)
DVAMKPPSVYLLPPTR
ELLLRSEPSK
VILCMPESMSIER + Carbamidomethyl (C)
IVDIIK
FIDGFK
TKEFYK
TVIEKCVDGK + Acrylamide (C)
TLVWINR
139
339
195
686
668
141
665
65
56
480.7627
613.8061
508.2808
892.4922
880.4761
481.2708
871.9214
425.2194
421.2608
959.5108
1225.5976
1014.5470
1782.9698
1758.9376
960.5270
1741.8282
848.4242
840.5070
959.5076
1225.6013
1014.5458
1782.9702
1758.9377
960.5240
1741.8311
848.4248
840.5069
3.35
-2.96
1.25
-0.21
-0.00
3.17
-1.66
-0.67
0.20
1
0
1
0
0
0
1
0
0
16
16
16
16
16
16
16
16
16
33
51
46
18
19
31
26
28
19
1
1
1
1
1
1
1
1
1
TIRYPDPV
NIAGTLMFDTK + Oxidation (M)
DNIDAAIKR
DVAMKPPSVYLLPPTR
HQGLPQEVLNENLLR
ITAGGATATAK
WATSEEGQKFIGDFK
VPMAMLR + 2 Oxidation (M)
IITPAQAK
12/9/2009
Page 18 of 22
466
453
659
12
483
310
623
887
646
577
492
699.8176
456.9208
865.4140
362.6973
712.8546
586.3393
563.3173
968.7997
857.4072
801.3873
720.8722
1397.6206
1367.7406
1728.8134
723.3800
1423.6946
1170.6640
1686.9301
2903.3773
1712.7998
1600.7600
1439.7298
1397.6245
1367.7409
1728.8141
723.3803
1423.6943
1170.6608
1686.9305
2903.3753
1712.8048
1600.7621
1439.7296
-2.75
-0.21
-0.38
-0.32
0.25
2.78
-0.24
0.67
-2.91
-1.26
0.14
1
0
0
0
1
1
1
0
1
0
0
16
16
16
15
15
15
15
15
15
15
15
16
18
27
23
28
30
17
27
28
27
34
1
1
1
1
1
1
1
1
1
1
1
YKGQDGNSEAAMK
QIGAEVEPGLLSR
AITEYK
NLAKSGDYNDSIK
ELLLRSEPSK
TGVDIKWTAVGTGAALK
NVSTGDVNVEMNAAPGVDLTQLLNNMR + 2 Oxidation (M)
FSVSGDYQLEDTLK
VDLAFLDSFASQK
688
136
17
643
698
315
773
574
667
16
351
468
739
3
895.4582
478.7364
367.7259
855.4391
908.9865
589.3425
663.3543
534.2940
871.9222
367.7132
620.3091
704.3688
964.9623
350.6971
1788.9018
955.4582
733.4372
1708.8636
1815.9584
1176.6704
1987.0411
1599.8602
1741.8298
733.4118
1238.6036
1406.7230
1927.9100
699.3796
1788.9046
955.4585
733.4374
1708.8573
1815.9618
1176.6689
1987.0370
1599.8620
1741.8311
733.4123
1238.6004
1406.7227
1927.9051
699.3803
-1.56
-0.31
-0.24
3.73
-1.86
1.33
2.06
-1.17
-0.75
-0.56
2.66
0.22
2.57
-0.93
0
0
0
1
0
0
0
0
1
0
0
0
0
0
15
15
15
15
15
15
15
15
15
15
15
15
15
15
37
24
12
29
35
9.2
31
35
33
39
43
56
33
38
1
1
1
1
1
1
1
1
1
1
1
1
1
1
SSASSITAIVPYFGYAR
YYMNPLR
IVDIFK
WEAFLAKEGAATPYR
SSIAQFPDVSSIEVIPK
MFILASVQLR
IAIDGAMPDLTLVLDICAK + Oxidation (M)
LLDKPLFTPDANTR
WATSEEGQKFIGDFK
EIFGIR
GDGDRPPAELGR
MIENENILYIR
YVLDIDYDVNLDETNK
IDDPIK
119
601
19
493
353
444
64
771
425
316
762
78
255
784
751
793
508
500
231
13
692
248
31
204
293
849
1
320
467.7711
547.9601
377.2016
720.8724
622.3079
676.3591
424.7650
663.3541
655.3229
589.8076
983.5451
432.7493
553.8062
1021.5050
650.6551
690.3725
735.3634
485.2692
531.7770
363.6782
600.6619
546.2810
397.2263
513.3087
575.3051
804.7467
350.1891
395.5560
933.5276
1640.8585
752.3886
1439.7302
1242.6012
1350.7036
847.5154
1987.0405
1308.6312
1177.6006
1965.0756
863.4840
1105.5978
2040.9954
1948.9435
2068.0957
1468.7122
1452.7858
1061.5394
725.3418
1798.9639
1090.5474
792.4380
1024.6028
1148.5956
2411.2183
698.3636
1183.6462
933.5284
1640.8603
752.3891
1439.7265
1242.6026
1350.7031
847.5167
1987.0414
1308.6310
1177.6013
1965.0723
863.4865
1105.6019
2040.9898
1948.9452
2068.0953
1468.7133
1452.7864
1061.5393
725.3418
1798.9676
1090.5441
792.4382
1024.6029
1148.5938
2411.2179
698.3639
1183.6423
-0.77
-1.10
-0.58
2.62
-1.13
0.41
-1.51
-0.49
0.19
-0.52
1.68
-2.84
-3.68
2.76
-0.88
0.18
-0.70
-0.44
0.13
0.05
-2.08
3.07
-0.14
-0.06
1.61
0.15
-0.37
3.23
0
1
0
1
1
1
0
1
1
1
1
0
1
1
0
0
0
1
0
0
1
0
0
1
1
1
0
0
15
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
48
38
35
39
23
44
24
32
37
47
19
62
38
42
42
28
36
20
58
26
33
72
96
38
63
45
25
36
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
AVGFNVISK
FFVAPFPEVFGKEK
MIGSFAK
GMFLENLMRVAK + 2 Oxidation (M)
EHEVTCKAAQK
KDAVDVSFETLK
LLASGIFK
AILDNFDEYKTLHPALK
DAGFRTTELDGK
TKTNLPEMTK + Oxidation (M)
LNPFEFIIGNEILYRK
DPAVPPLR
KVVFELDEK
NDLETEITRHNNDVIMK
IDELMDAVDSYIPTPVR + Oxidation (M)
IIAVEPEASPVLSGGNPGPHK
AIYTWVANTMQR + Oxidation (M)
FYDVIGKELIEK
AEYPDIQVK
LFSCVS + Acrylamide (C)
LLKDIISLDEGDNINK
VQQTQMVNK + Oxidation (M)
VGIDYVK
ARVEVNIPK
RSPITEQYR
ALGLEEDIAKEQLEAANEEIAR
EIFYK
MFEVLPPPVR
254
456
28
705
100
862
271
373
724
599
359
747
415
524
550.7816
686.8786
393.7343
919.4659
449.2608
851.4005
559.7958
627.3222
937.4593
545.6246
623.2896
647.6495
433.9271
745.3857
1099.5486
1371.7426
785.4540
1836.9172
896.5070
2551.1797
1117.5770
1252.6298
1872.9040
1633.8520
1244.5646
1939.9267
1298.7595
1488.7568
1099.5484
1371.7470
785.4548
1836.9105
896.5079
2551.1782
1117.5768
1252.6299
1872.9040
1633.8498
1244.5642
1939.9244
1298.7558
1488.7532
0.21
-3.17
-0.96
3.68
-0.99
0.57
0.25
-0.04
0.05
1.34
0.38
1.19
2.85
2.44
1
1
0
1
0
0
0
0
0
1
0
1
1
1
14
13
13
13
13
13
13
13
13
13
13
13
13
13
50
53
20
54
26
39
92
47
59
62
29
58
27
67
1
1
1
1
1
1
1
1
1
1
1
1
1
1
YYMNPLRK + Oxidation (M)
EVNATQRLSLNK
VYIVHR
NSGVDKEAGYESVELIK
NINSKPPK
TTDVTGSITLPEGTEMVMPGDNVR + 2 Oxidation (M)
ENVQLFSGPK
EDAHVIAELEK
ILNGAMSSSSQFYNNIK
AQTTIQDPVFQKMK
AGCDPIVAPCSGR
CANSICNGASAINFKSAK + 2 Acrylamide (C)
IQNIIKVEQSK
IDAVSVESTRDAAR
584
116
257
289
634
179
810
380
429
807.3376
466.7562
554.2787
382.5554
849.4526
499.3111
723.0745
630.2974
439.5853
1612.6606
931.4978
1106.5428
1144.6444
1696.8906
996.6076
2166.2017
1258.5802
1315.7341
1612.6650
931.4975
1106.5430
1144.6452
1696.8865
996.6080
2166.2010
1258.5798
1315.7356
-2.70
0.41
-0.15
-0.70
2.44
-0.34
0.32
0.34
-1.16
1
0
0
1
1
1
0
0
0
13
13
13
13
13
13
13
13
13
9.6
77
86
39
46
16
15
38
36
1
1
1
1
1
1
1
1
1
DMFNAYMSGDFRK + 2 Oxidation (M)
VSTGSKPSVA
FIMNNDPLK + Oxidation (M)
VLSAADKNTVK
RIPHTQAMGMLLTGR + Oxidation (M)
LIKPGDKAR
LLLVFVEYTESNCLLLIK + Carbamidomethyl (C)
CAQMLADHGVK + Acrylamide (C); Oxidation (M)
SCVGIPIVMLLR + Oxidation (M)
12/9/2009
Page 19 of 22
55
173
370
379
639
536
368
187
687
711
713
421.2605
496.7980
626.2787
630.2971
852.3983
756.8579
625.7659
505.2692
892.9395
927.4636
929.4622
840.5064
991.5814
1250.5428
1258.5796
1702.7820
1511.7012
1249.5172
1008.5238
1783.8644
1852.9126
1856.9098
840.5069
991.5815
1250.5424
1258.5764
1702.7838
1511.7013
1249.5179
1008.5240
1783.8628
1852.9175
1856.9124
-0.52
-0.00
0.37
2.56
-1.05
-0.06
-0.55
-0.14
0.91
-2.62
-1.38
0
1
0
0
0
1
0
1
0
1
1
13
13
13
13
13
13
12
12
12
12
12
37
21
34
41
51
48
12
56
66
69
71
1
1
1
1
1
1
1
1
1
1
1
ILVSPANK
KTLPAPTHK
MPEDCVPTFR + Carbamidomethyl (C)
GWEAPEVCAAR + Acrylamide (C)
GFELNYEDGEYIVR
AGNTGMQMGGWFKK
TCRPEEECR + Acrylamide (C); Carbamidomethyl (C)
ETLASYGRL
QYFEPAQTSGEISLSK
EEMVRNYICVAITQK + Carbamidomethyl (C)
CQKHLMGEDLESLNLK
203
670
338
582
648
275
894
640
804
188
592
723
458
546
512.2403
588.3243
613.3383
802.4487
572.2999
563.7855
1090.5210
852.9536
714.6879
505.2874
541.9551
467.7871
691.8444
769.3240
1022.4660
1761.9511
1224.6620
1602.8828
1713.8779
1125.5564
3268.5412
1703.8926
2141.0419
1008.5602
1622.8435
1867.1193
1381.6742
1536.6334
1022.4637
1761.9513
1224.6615
1602.8837
1713.8832
1125.5601
3268.5380
1703.8916
2141.0429
1008.5604
1622.8450
1867.1216
1381.6734
1536.6297
2.27
-0.12
0.46
-0.53
-3.11
-3.21
0.97
0.61
-0.48
-0.13
-0.96
-1.26
0.62
2.45
1
1
1
0
1
0
1
0
1
1
1
0
0
0
12
12
12
12
12
12
12
12
12
12
12
11
11
11
61
43
48
39
74
80
59
66
75
33
89
3
76
12
1
1
1
1
1
1
1
1
1
1
1
1
1
1
ECLKCGTR + 2 Carbamidomethyl (C)
YKISPDTQGVLVTDVK
KHINTWVAEK
GALLTMNCLAILVGK + Acrylamide (C); Oxidation (M)
IARLIQEVPTGCSDR + Carbamidomethyl (C)
LLECLGHGER
IAGEVPEKDSWYMQTGQGMLATINAAAEQK + 2 Oxidation (M)
VMGSNEYVLNLIQPK
VLYSSEAFPTNFRDPEAAK
ITPPDKNPK
GVLTVGECPHALKDK + Carbamidomethyl (C)
MDVLAVVGNLAVIIVITK
NVEKPVQMMFK + 2 Oxidation (M)
SGNSCLSGDSHGAMK + Acrylamide (C); Oxidation (M)
824
277
844
612
772
678
677
649
412
410
45
645
472
620
358
690
405
658
628
852
765
691
276
774
704
35
44
549
740.0666
564.2774
790.7598
832.4182
663.3541
591.0036
591.0031
857.9468
650.3865
644.8173
409.7238
856.9593
710.8640
843.3916
623.2847
598.3222
641.8493
577.2431
565.6088
825.0756
660.3282
897.9113
563.7857
663.3545
915.9687
399.1930
409.2365
517.2689
2217.1780
1126.5402
2369.2576
1662.8218
1987.0405
1769.9890
1769.9875
1713.8790
1298.7584
1287.6200
817.4330
1711.9040
1419.7134
1684.7686
1244.5548
1791.9448
1281.6840
1728.7075
1693.8046
2472.2050
1977.9628
1793.8080
1125.5568
1987.0417
1829.9228
796.3714
816.4584
1548.7849
2217.1827
1126.5367
2369.2591
1662.8174
1987.0343
1769.9941
1769.9941
1713.8733
1298.7558
1287.6169
817.4334
1711.9079
1419.7146
1684.7735
1244.5530
1791.9519
1281.6825
1728.7083
1693.8028
2472.2146
1977.9585
1793.8027
1125.5601
1987.0370
1829.9193
796.3715
816.4593
1548.7891
-2.14
3.15
-0.64
2.65
3.11
-2.88
-3.72
3.35
2.06
2.43
-0.42
-2.27
-0.84
-2.87
1.51
-3.99
1.21
-0.49
1.02
-3.89
2.17
2.96
-2.85
2.37
1.92
-0.09
-1.01
-2.76
1
0
1
1
1
1
1
1
1
0
0
1
0
0
0
0
0
1
1
1
0
1
0
0
0
0
0
1
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
10
10
10
10
10
10
54
68
46
81
67
34
35
77
43
84
46
69
1.1e+002
64
34
68
69
16
1e+002
1e+002
99
1e+002
1.1e+002
87
1e+002
46
1.3e+002
1.5e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
VKVMAFLNALLDAEGINLDR + Oxidation (M)
LNSHTSDTPR
GYKVAVLAVDPSSGVTHGSILGDK
VDEFLDMPLKEAEK
ACLASTRAQAIHVSSVVMK + Oxidation (M)
VQAAPFTLIGGHLYKR
VQAAPFTLIGGHLYKR
RGVLGGHDHMHILEK + Oxidation (M)
LESLKVPASSLR
CYNDYLVTVK + Acrylamide (C)
VDASWLK
RILMPESWIGEPLR + Oxidation (M)
YVNWIQQTIAAN
SCGNICAIIYCLER + Acrylamide (C); Carbamidomethyl (C)
TICVFCGSSSGK + Carbamidomethyl (C)
TLQFIPVEYLNTQAR
FVITSNMMLVK
YCTDPATAEDKCDR + 2 Acrylamide (C)
LCHTVTAADGTRMYR
GASQYNWTDSDVRVAVAHAVGAAK
GIDSGLYLYVDFHGHASK
EETSNSEEQATKQTGR
VVHEACIER + Acrylamide (C)
IAIDGAMPDLTLVLDICAK + Oxidation (M)
EGGVITLTSMVVPDAEGR
ISDYGSR
TIDDIIK
TIMETPEKLVGGMK + Oxidation (M)
160
76
708
728
550
395
722
447
674
699
407
51
66
746
490.7744
430.7474
923.9652
942.9492
775.4001
636.3597
930.4968
452.9231
589.9437
909.9709
642.4001
417.2356
425.7610
970.9703
979.5342
859.4802
1845.9158
1883.8838
1548.7856
1270.7048
1858.9790
1355.7475
1766.8093
1817.9272
1282.7856
832.4566
849.5074
1939.9260
979.5338
859.4803
1845.9216
1883.8821
1548.7817
1270.7002
1858.9829
1355.7482
1766.8112
1817.9312
1282.7874
832.4555
849.5072
1939.9205
0.43
-0.12
-3.11
0.95
2.53
3.65
-2.09
-0.57
-1.07
-2.17
-1.33
1.34
0.25
2.84
1
0
1
0
1
1
0
0
0
1
1
0
1
0
10
10
10
10
10
10
10
10
10
10
10
10
10
10
95
43
1.1e+002
96
1.5e+002
67
98
80
90
1.1e+002
15
1.6e+002
62
1.3e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
NDVYKTLK
GPSIYVPK
MKLQMEEPSEITAVPK + Oxidation (M)
GVSSSNTLSSSNTLSSSNR
TLQMETNKLNETK
CLNVPVLVCRR
NVPQEEGIVPFVFVGTK
MLNETLGPQLIK
IFTSDADFSGITNDHK
FIDGHELDILKDFTR
GRLPALLFAVAR
VAINGFGR
LVRSFTK
MLEMEFPLAALAGTTCR + Acrylamide (C); Oxidation (M)
714
544
830
780
416
299
118
72
354
929.9438
759.3855
759.0690
1006.4890
650.3871
581.7907
467.7306
428.7785
622.8314
1857.8730
1516.7564
2274.1852
2010.9634
1298.7596
1161.5668
933.4466
855.5424
1243.6482
1857.8719
1516.7562
2274.1799
2010.9568
1298.7558
1161.5666
933.4450
855.5430
1243.6448
0.59
0.16
2.32
3.30
2.99
0.21
1.76
-0.59
2.74
0
0
1
1
1
0
1
0
0
10
10
10
10
10
10
10
10
9
3.9e+002
1.2e+002
86
1.1e+002
60
1.8e+002
1.2e+002
33
1.5e+002
1
1
1
1
1
1
1
1
1
FINNCSPNFYIIDNK + Carbamidomethyl (C)
GYGWIPVDPADVTK
VCALARQMVGGHYVQMAIIK + Acrylamide (C); Oxidation (M)
ADNYVTKLTNDLAALSMDG
ATGAGEGIIALKAK
IQEDGQVFLN
MSNAERAR
LLVLVDGK
EDDLWLIVNK
12/9/2009
Page 20 of 22
374
797
406
813
755
509
725
554
131
189
881
627.3484
697.6881
642.3406
726.6766
981.9756
735.3649
937.9512
783.3979
475.7287
505.8155
940.8475
1252.6822
2090.0425
1282.6666
2177.0080
1961.9366
1468.7152
1873.8878
1564.7812
949.4428
1009.6164
2819.5207
1252.6816
2090.0354
1282.6629
2177.0059
1961.9308
1468.7207
1873.8880
1564.7807
949.4440
1009.6172
2819.5103
0.53
3.39
2.88
0.96
3.00
-3.69
-0.07
0.38
-1.18
-0.70
3.67
0
0
1
0
1
0
1
0
1
0
1
9
9
9
9
9
9
9
9
9
9
9
95
1.4e+002
1.1e+002
1e+002
1.4e+002
1.3e+002
1.2e+002
2.2e+002
96
22
41
1
1
1
1
1
1
1
1
1
1
1
TVLGNFSAFVAK
DLVGMGYQILAISPESPER + Oxidation (M)
EHLAVTRTDNK
GEQISNIGSQDMNNEVWLK + Oxidation (M)
VGGMVCASCPPRIEMSIGR
VPMPPPPTYAMPR + Oxidation (M)
AMGPAFGDEEREPLLDK
ENVFLEEINALMK + Oxidation (M)
MFDPRER
AEGLVPALLK
VGDTIEIVGIKPTQTTTVTGVEMFRK
717
635
568
371
650
748
817
825
788
768
580
666
791
783
929.9535
566.6386
794.8633
626.3580
572.3003
970.9712
737.3734
744.0206
1029.5010
661.3437
534.9459
581.6172
690.3201
680.3219
1857.8924
1696.8940
1587.7120
1250.7014
1713.8791
1939.9278
2209.0984
2229.0400
2056.9874
1981.0093
1601.8159
1741.8298
2067.9385
2037.9439
1857.8891
1696.8903
1587.7086
1250.6983
1713.8794
1939.9230
2209.1015
2229.0437
2056.9809
1981.0051
1601.8195
1741.8240
2067.9432
2037.9374
1.81
2.15
2.15
2.54
-0.17
2.48
-1.42
-1.67
3.16
2.11
-2.29
3.33
-2.30
3.19
1
1
0
0
0
1
1
1
0
1
1
1
1
1
9
9
9
9
9
9
9
8
8
8
8
8
8
8
1.4e+002
1.1e+002
87
80
1.4e+002
1.7e+002
1.7e+002
1.2e+002
1.4e+002
1.6e+002
1.6e+002
1.5e+002
1.1e+002
1.3e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
REVEGGEGVETMVVDPR
QVRINCIRPNNNTR
HDSELTLEVEDCAK
RPPILIGGDGEK
MALIAQLVDDVMVHK + 2 Oxidation (M)
CAKSASASTLEEAMEVVK + Acrylamide (C); Oxidation (M)
YNVGNPGSGNEVSTLEVFKAK
DEYDRYDDILNTLSELQK
MLVFGGDSILVTDLECSSR + Oxidation (M)
SSISNSQVKQFSCQIVR + Acrylamide (C)
QLVDMAAKVGADVNR + Oxidation (M)
HGGCGAQQPKISIDGMK + Oxidation (M)
MDGDNVAADFGNRFAAEIR
CCDNVPRPSHPSWRTAR + Carbamidomethyl (C)
463
707
323
806
561
337
754
581
566
673
372
247
517
712
763
548
303
418
890
619
322
767
252
758
706
742
331
889
695.8466
615.3245
598.3441
1073.5080
788.3851
610.8532
980.9529
535.3014
791.9055
883.8948
627.2769
364.2083
739.8138
927.4636
656.0325
517.2687
584.7729
650.3873
984.7789
842.9007
596.8193
991.5106
550.2448
655.7057
922.4821
645.3196
605.3169
973.4703
1389.6786
1842.9517
1194.6736
2145.0014
1574.7556
1219.6918
1959.8912
1602.8824
1581.7964
1765.7750
1252.5392
1089.6031
1477.6130
1852.9126
1965.0757
1548.7843
1167.5312
1298.7600
2951.3149
1683.7868
1191.6240
1981.0066
1098.4750
1964.0953
1842.9496
1932.9370
1208.6192
2917.3891
1389.6810
1842.9588
1194.6720
2144.9935
1574.7610
1219.6925
1959.8926
1602.8837
1581.7960
1765.7802
1252.5434
1089.6039
1477.6112
1852.9167
1965.0683
1548.7896
1167.5302
1298.7598
2951.3138
1683.7814
1191.6248
1981.0091
1098.4739
1964.0877
1842.9509
1932.9330
1208.6190
2917.3859
-1.67
-3.87
1.34
3.68
-3.39
-0.51
-0.70
-0.85
0.29
-2.91
-3.32
-0.71
1.24
-2.16
3.74
-3.45
0.89
0.19
0.37
3.22
-0.59
-1.24
1.02
3.84
-0.70
2.07
0.23
1.08
0
0
1
0
0
1
1
1
0
1
0
1
0
0
1
1
1
0
1
0
1
1
0
1
1
1
0
1
8
8
8
8
8
8
8
8
8
8
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
1.9e+002
1.8e+002
81
1.5e+002
1.8e+002
89
1.1e+002
1.5e+002
1.6e+002
1.1e+002
1e+002
1.5e+002
41
2.1e+002
95
3.5e+002
1.5e+002
1.1e+002
1.4e+002
2.5e+002
2.8e+002
2.1e+002
58
66
2.2e+002
2.5e+002
3.1e+002
2.1e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
LVEVEATLCDNK + Carbamidomethyl (C)
ARPSIADVWDVLSSATR
NIGLIAHDSKK
YNIQIIEDSCEALGSEYK + Acrylamide (C)
GKPVKYTGGSVK
NLLSMAGEMKMMPFGAGK + 3 Oxidation (M)
LGMKPVDGVTKVCIK + Oxidation (M)
ITTSAYMPTEIEVK
WECVSGSGTKNSNQNR
EPDPNAFYGLM
VKVPMMTLR + Oxidation (M)
AVTGSCHCVECGGK + Acrylamide (C); Carbamidomethyl (C)
EINTDHVGLVAESNIDK
TVRLTVAEAFANYLAAQK
IGVLREYVEDGSGR
SRETATSSACR
FLLHSLILESK
AAVHAKNCQTTTGNGNPVECSQSESSFK + Carbamidomethyl (C)
FVNPTPCTVEYTDK + Acrylamide (C)
LKGGYVANENK
LVMRNGQFEAALFLNDK + Oxidation (M)
MHCFFVSR + Carbamidomethyl (C); Oxidation (M)
LLVVDEHNRCVGLITVK + Carbamidomethyl (C)
LMPVQLDESGGAVLRDK + Oxidation (M)
WTEAVDPYARAAAVNGDK
GYVHVGYSISK
YSINYIPLGCCRLSWNCANGMILR + 2 Acrylamide (C); Oxidation (M)
135
99
886
731
265
664
721
795
733
171
749
551
610
734
478.2534
448.7177
968.1383
632.6478
556.3164
581.6165
620.6668
1042.0090
636.0144
496.7208
972.9554
775.4008
829.3951
953.9545
954.4922
895.4208
2901.3931
1894.9216
1110.6182
1741.8277
1858.9786
2082.0034
1905.0214
991.4270
1943.8962
1548.7870
1656.7756
1905.8944
954.4923
895.4222
2901.3969
1894.9207
1110.6146
1741.8271
1858.9836
2082.0027
1905.0248
991.4281
1943.8903
1548.7826
1656.7739
1905.8890
-0.06
-1.47
-1.31
0.44
3.32
0.33
-2.70
0.38
-1.77
-1.02
3.04
2.86
1.08
2.85
0
1
0
0
1
1
1
1
0
0
1
1
1
1
6
6
6
6
6
6
6
6
6
6
6
5
5
5
1.7e+002
2.7e+002
2.4e+002
3.1e+002
1.9e+002
2.5e+002
2.4e+002
2.9e+002
1.9e+002
87
2.4e+002
4.4e+002
2.9e+002
2.8e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
ALNVFSPHA
MFGEERK
EIMNFLGTVFQVELGDYYAAYIAMK + Oxidation (M)
NMDQFVATGVTGSIVSNR
LSDTGRVHVK
LDLDAAGSYSRNDAFK
GHLCLSSLKVVEAPGHR + Carbamidomethyl (C)
VEIIQTLVNNYPDIFK
TPDEMIDR + Oxidation (M)
VDIMTFCQQKAAQSCK + Acrylamide (C); Carbamidomethyl (C); Oxidation (M)
KTIQGPVGMALCGMK + Oxidation (M)
SMEEMVDKYPLTAK + Oxidation (M)
ILNNEYEDMRLPDER
875
467
125
503
701
808
481
507
865
921.1356
703.8584
471.7572
731.3895
608.7024
716.0084
712.8361
734.3590
859.7443
2760.3850
1405.7022
941.4998
1460.7644
1823.0854
2145.0034
1423.6576
1466.7034
2576.2111
2760.3831
1405.7062
941.4971
1460.7657
1823.0815
2144.9983
1423.6613
1466.7075
2576.2107
0.67
-2.82
2.95
-0.84
2.13
2.37
-2.57
-2.77
0.16
1
1
1
1
1
0
0
0
0
5
5
5
5
5
5
5
5
5
3.4e+002
3.7e+002
9.3e+002
3.8e+002
23
2.7e+002
2.7e+002
4.4e+002
2.9e+002
1
1
1
1
1
1
1
1
1
VWDIVSRFGGLDADIDSLNLSQGQR
VNGELRQYGNTR
WQEGVKVP
TELQELKLNMAR + Oxidation (M)
LLQRPCVTIKIGGQLK + Carbamidomethyl (C)
HCNLAELSALVHMSGSFEK + Carbamidomethyl (C); Oxidation (M)
NCQSGTLSSADITK
QIIADFIEMESR + Oxidation (M)
EIDVILMDLQMPICNGQEACIR + Carbamidomethyl (C); Oxidation (M)
12/9/2009
Page 21 of 22
547
408
822
641
85
787
816
465
856
864
618
772.8525
428.6026
1109.5950
854.3761
435.2760
685.6523
737.3731
699.2883
836.1295
855.7537
840.8830
1543.6904
1282.7860
2217.1754
1706.7376
868.5374
2053.9351
2209.0975
1396.5620
2505.3667
2564.2393
1679.7514
1543.6903
1282.7860
2217.1794
1706.7392
868.5382
2053.9310
2209.0945
1396.5612
2505.3665
2564.2475
1679.7494
0.09
-0.03
-1.77
-0.93
-0.86
2.00
1.34
0.61
0.07
-3.21
1.20
0
1
1
1
1
1
1
0
0
1
1
5
5
5
4
4
4
4
4
4
4
4
1.9e+002
48
2.6e+002
1.6e+002
1.1e+002
3.1e+002
4.4e+002
55
1.2e+002
4.3e+002
2.5e+002
1
1
1
1
1
1
1
1
1
1
1
ATGQEFSDFGAASTR
KPDETLVLLKK
VQVFPSAQLIDDDRVFGALK
NTVGSFECSCQKGYK + Carbamidomethyl (C)
IVKAPVDK
DTVSSQNGIPAEFMCRAR + Carbamidomethyl (C); Oxidation (M)
KLGVDTVGFLMMAHMISAEK + 2 Oxidation (M)
CEEAEHCVHAR + 2 Carbamidomethyl (C)
AIPPLAIGVCSWSLQVTNVPELK + Acrylamide (C)
GSIFQSSSDSECIIHLMARSIQR
ASEECGEGLKAVCEK + Acrylamide (C); Carbamidomethyl (C)
807
877
413
741
828
842
883
569
829
88
212
879
851
891
716.0079
935.7980
650.3868
965.4534
744.0427
773.7079
945.4628
796.8904
744.7469
436.7747
516.3142
705.8846
816.4058
990.1147
2145.0019
2804.3722
1298.7590
1928.8922
2229.1063
2318.1019
2833.3666
1591.7662
2231.2189
871.5348
1030.6138
2819.5093
2446.1956
2967.3223
2145.0022
2804.3666
1298.7558
1928.8963
2229.0987
2318.1026
2833.3754
1591.7705
2231.2274
871.5378
1030.6175
2819.5069
2446.1881
2967.3268
-0.13
1.99
2.53
-2.11
3.41
-0.32
-3.11
-2.65
-3.82
-3.44
-3.57
0.83
3.06
-1.54
1
0
1
0
0
1
0
0
0
1
0
0
1
0
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3.7e+002
3.9e+002
2.1e+002
3.9e+002
4.5e+002
3.9e+002
4.1e+002
6.1e+002
1.6e+002
2.3e+002
1.5e+002
1.7e+002
4.8e+002
2.2e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
VPLVSRDQCSHQQSYGDR + Acrylamide (C)
VSQLALFDQFPYTHHMECGVLLEK
QNTAISPLKISK
SIANIIPNDGTDENDVDK
MLAGELYLPGGPELAADEAANK
GPVKVLYVDDNNENNGSELDK
TFGVATGPFGAIIAWCIAGGGMYMLAR + Acrylamide (C); 2 Oxidation (M)
AALDVMHYFDPSVK
IAEAAGGVVGVGLGGASVPPALVDR
ELKGILSL
LEVVVFLGR
TVIYSSPLGNNVVLTGTVPAGNATFTVK
LPNMCRALDISTLGMYGMFLK + Carbamidomethyl (C); Oxidation (M)
CEVCGNIVEVLNGGIGELVCCNQDMK + 3 Acrylamide (C); Oxidation (M)
750
861
69
552
831
516
672
854
718
874
882
606
48
168
419
869
127
683
855
375
841
876
716
617
101
867
730
868
972.9592
846.4105
428.2616
777.8377
759.0692
738.8998
883.8941
836.1273
929.9537
921.1336
941.4748
550.0007
414.7615
494.3007
650.3878
867.7704
472.2877
892.4285
836.1276
627.7689
769.6681
921.7055
929.9447
840.8813
450.2747
860.7603
632.6470
867.7692
1943.9038
2536.2097
854.5086
1553.6608
2274.1858
1475.7850
1765.7736
2505.3601
1857.8928
2760.3790
2821.4026
1646.9803
827.5084
986.5868
1298.7610
2600.2894
942.5608
1782.8424
2505.3610
1253.5232
2305.9825
2762.0947
1857.8748
1679.7480
898.5348
2579.2591
1894.9192
2600.2858
1943.9081
2536.2166
854.5086
1553.6668
2274.1889
1475.7885
1765.7763
2505.3528
1857.8940
2760.3752
2821.3956
1646.9832
827.5116
986.5873
1298.7598
2600.2864
942.5611
1782.8458
2505.3690
1253.5276
2305.9775
2762.1002
1857.8679
1679.7468
898.5348
2579.2512
1894.9220
2600.2947
-2.19
-2.74
0.03
-3.81
-1.39
-2.34
-1.51
2.90
-0.59
1.36
2.47
-1.75
-3.86
-0.41
0.95
1.13
-0.21
-1.86
-3.19
-3.50
2.17
-2.00
3.72
0.74
0.03
3.05
-1.51
-3.42
0
0
1
0
1
1
1
0
1
1
1
1
0
1
0
1
1
1
0
0
1
1
1
1
1
1
1
1
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
4.1e+002
4.9e+002
2.1e+002
1.9e+002
4.1e+002
5.2e+002
3.2e+002
1.8e+002
5.7e+002
6.4e+002
5.3e+002
36
4.3e+002
2.8e+002
93
6.4e+002
6.1e+002
6.7e+002
2.4e+002
2.3e+002
2e+002
45
2.7e+003
4.4e+002
9.2e+002
8.1e+002
9.1e+002
8.6e+002
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
LTPDNMTGHELVDMLSR + Oxidation (M)
MGSVGMCLLLSVCLACLACIAGHK + Acrylamide (C); Carbamidomethyl (C); Oxidation (M)
RVPALSGR
ACGTNDEAFAELEK + Carbamidomethyl (C)
TQLGLGLADDGAEICITLSTRK
LEVHRFGITGYGK
KHTGEKPYECSECGK + Acrylamide (C)
VLGSTLVVGLLVGMGYFAFMLFR + Oxidation (M)
DLIRYFCMPCKPTK + Acrylamide (C); Carbamidomethyl (C); Oxidation (M)
LADAATATEGLQMAAEDLPSEFVVRR
AELFSTEQLAELPILDRCFDAVDAR
VAKVTALAHDLAVALR
VIPITASK
LKAAGATLSR
NLILLTGPPFSK
RGLPSGCLIISGDDGSADETVGAALR + Acrylamide (C)
AVIIDTRR
ELCKGISEVNYNSEK + Acrylamide (C)
QLLLADESAIPAALAILESSPDLR
MMMETMDPIR
IISFATNMCHKCGMQCVYG + Acrylamide (C); 2 Carbamidomethyl (C); Oxidation (M)
DSWMSTSSMDTAMDMSIGMYAKER + 2 Oxidation (M)
TFQLGAGREDDYLMSR
DGSGGCVRIQGTNTCR + Carbamidomethyl (C)
NKRPGSLK
MMFELHGVIPSETEENLFRIR + 2 Oxidation (M)
AGQEEGLAAHVVHYRCR
DLQSIGMICALAYSVCGMLRLVR + Carbamidomethyl (C); 2 Oxidation (M)
735
866
292
23
870
7
8
11
42
96
112
184
426
513
478.7913
860.4259
574.2829
384.2353
875.4474
357.6368
357.6369
362.2219
406.2484
442.2765
458.7875
502.8146
656.7883
491.2990
1911.1361
2578.2559
1146.5512
766.4560
2623.3204
713.2590
713.2592
722.4292
810.4822
882.5384
915.5604
1003.6146
1311.5620
1470.8752
1911.1332
2578.2526
1146.5517
766.4589
2623.3138
1.51
1.27
-0.38
-3.67
2.49
1
1
1
0
0
1
1
0
0
0
50
9.1e+002
8.4e+002
2.2e+002
9.3e+002
1
1
1
1
1
ELKEIPIIDIFDILLK
LKPAETSVDRSFIDYGVFFECR
VNTDGAEGGSKL
EIPAIPK
MGMEVFATLAGATIQGQIVGVYHAK + 2 Oxidation (M)
541
613
627
663
700
740
753
757
777
505.9743
555.6757
564.6763
579.3520
456.7785
483.0479
489.7979
983.0544
500.8037
1514.9011
1664.0053
1691.0071
1735.0342
1823.0849
1928.1625
1955.1625
1964.0942
1999.1857
12/9/2009
782
801
505.0618
533.8229
Page 22 of 22
2016.2181
2131.2625
Search Parameters
Type of search
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
MS/MS Ion Search
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
894
12/9/2009
Select Summary Report (N_18_7_09_10.RAW relex2)
Page 1 of 18
User
:
Email
:
Search title
:
MS data file
:
Database
:
Timestamp
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
Protein hits
:
LTQFT Daemon
N_18_7_09_10.RAW relex2
D:\Data\Mark\18_7-09\N_18_7_09_10.RAW
9 Dec 2009 at 04:21:42 GMT
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
1500
gi|171855138 RecName: Full=Elongation factor Ts; Short=EF-Ts
gi|28317
gi|126165236 hypothetical protein LOC617667 [Bos taurus]
gi|157164740 outer membrane fibronectin-binding protein [Campylobacter concisus 13826]
gi|154173809 translocation protein TolB [Campylobacter curvus 525.92]
gi|157164833 phosphopyruvate hydratase [Campylobacter concisus 13826]
gi|158604943 aspartate-semialdehyde dehydrogenase [Campylobacter concisus 13826]
gi|157164054 cytochrome c oxidase, heme b and copper-binding subunit, membrane-bound [Campylobacter concisus 13826]
gi|181402
gi|157165008 transketolase A [Campylobacter concisus 13826]
gi|1942778
gi|136429
gi|157164277 glyceraldehyde-3-phosphate dehydrogenase, type I [Campylobacter concisus 13826]
gi|122411
gi|47523270 alpha-1-antichymotrypsin 2 [Sus scrofa]
gi|157165527 hydrogenase-4 component I [Campylobacter concisus 13826]
gi|225632
casein alphaS1
gi|157164634 type II secretion system protein E [Campylobacter concisus 13826]
gi|122614
gi|2232299
gi|157164122 DNA polymerase III subunit beta [Campylobacter concisus 13826]
gi|6320157
Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick typ
gi|157165398 argininosuccinate synthase [Campylobacter concisus 13826]
gi|157163909 serine hydroxymethyltransferase [Campylobacter concisus 13826]
gi|32267367 putative C4-dicarboxylatebinding periplasmic protein [Helicobacter hepaticus ATCC 51449]
gi|157165776 putative carbon storage regulator-like protein [Campylobacter concisus 13826]
gi|157165509 DNA-directed RNA polymerase subunit alpha [Campylobacter concisus 13826]
gi|157165191 adenylosuccinate synthetase [Campylobacter concisus 13826]
gi|291470
gi|157164944 sigma 54 modulation protein/ribosomal protein S30EA [Campylobacter concisus 13826]
gi|149699777 PREDICTED: similar to Haptoglobin precursor [Equus caballus]
gi|157165110 hydrogenase accessory protein HypB [Campylobacter concisus 13826]
gi|157164063 3-oxoacyl-(acyl carrier protein) synthase II [Campylobacter concisus 13826]
gi|15027001 immunoglobulin gamma 4 heavy chain constant region [Equus caballus]
gi|157164697 phosphoglycerate kinase [Campylobacter concisus 13826]
12/9/2009
gi|27806941
gi|158604987
gi|154174678
gi|157165029
gi|157163983
gi|157164875
gi|157164923
gi|157164030
gi|229552
gi|157164579
gi|157164144
gi|27807349
gi|123506
gi|504
gi|28603766
gi|154173772
gi|154175359
gi|157165526
gi|157164168
gi|154173771
gi|154175458
gi|27806963
gi|157165326
gi|157165403
gi|157165668
gi|218459
gi|157164887
gi|112542
gi|154173631
Page 2 of 18
aminoacyl-histidine dipeptidase [Campylobacter concisus 13826]
aspartate kinase [Campylobacter concisus 13826]
glutamate-1-semialdehyde aminotransferase [Campylobacter concisus 13826]
albumin
serpin peptidase inhibitor, clade G, member 1 [Bos taurus]
serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 [Bos taurus]
NifS family cysteine desulfurase [Campylobacter curvus 525.92]
citrate (Si)-synthase [Campylobacter curvus 525.92]
general glycosylation pathway protein [Campylobacter concisus 13826]
phosphoribosylaminoimidazole synthetase [Campylobacter concisus 13826]
phosphoglycerate kinase [Campylobacter curvus 525.92]
Cys/Met metabolism PLP-dependent enzyme superfamily protein [Campylobacter concisus 13826]
Pir2p [Saccharomyces cerevisiae]
NifS family cysteine desulfurase [Campylobacter concisus 13826]
51K outer membrane protein - Wolinella recta (ATCC 33238) (fragment)
171
3
1.75 %
300
3
1.00 %
Select Summary Report
Format As
Help
Select Summary (protein hits)
Standard scoring
Show sub-sets 0
Require bold red
Show pop-ups
MudPIT scoring
Suppress pop-ups
1.
gi|157164629
Mass: 43586
Score: 1055
Queries matched: 61
emPAI: 7.30
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
88
417.2398
832.4650
832.4655 -0.49
0
25
3.5
1
R.TVGSGVVSK.I 87
133
434.2558
866.4970
866.4974 -0.38
0
41
0.041
1
R.EHILLSR.Q 134
351
524.7382 1047.4618 1047.4621 -0.27
0
51
0.0046
1
K.AGQDGEWSAK.I 350
365
530.3113 1058.6080 1058.6084 -0.35
1
25
2
3
R.GTVVTGRIEK.G
532
599.8213 1197.6280 1197.6281 -0.07
0
27
1.5
1
K.FEGEVYILTK.E
673
652.3826 1302.7506 1302.7507 -0.04
0
68 4.8e-005
1
K.TTLTAAISAVLSR.K 674
874
747.4129 1492.8112 1492.8112
0.02
0
43
0.032
1
R.QVGVPYIVVFMNK.A 873
886
755.4104 1508.8062 1508.8061
0.07
0
(34)
0.32
1
R.QVGVPYIVVFMNK.A 887 888
1040
845.4072 1688.7998 1688.8005 -0.41
1
62
0.0007
1
1053
848.9777 1695.9408 1695.9407
0.10
0
71 4.3e-005
1
R.ISVELIAPVALEEGTR.F 1052 1054
1061
851.9070 1701.7994 1701.7992
0.15
0
(79)
1e-005
1
K.EMDQGEAGDNVGVLLR.G 1060
1065
854.3765 1706.7384 1706.7383
0.07
1
55
0.0013
1
K.DYDNIDNAPEEKER.G
1089
859.9043 1717.7940 1717.7941 -0.04
0
102 4.8e-008
1
1123
590.2667 1767.7783 1767.7787 -0.27
0
23
3.2
1
R.HYAHVDCPGHADYVK.N
1171
915.9536 1829.8926 1829.8942 -0.82
1
67
0.00021
1
R.KEMDQGEAGDNVGVLLR.G
1183
924.4789 1846.9432 1846.9425
0.43
1
59
0.0015
1
R.GITIATSHIEYETEKR.H 1181 1182
1261
650.6620 1948.9642 1948.9638
0.19
0
(32)
0.85
1
K.IMELMDAVDSYIPTPVR.A 1259 1260
1275
983.4861 1964.9576 1964.9587 -0.55
0
(64) 0.00045
1
K.IMELMDAVDSYIPTPVR.A 1274
1277
983.4867 1964.9588 1964.9587
0.06
0
(51)
0.011
1
K.IMELMDAVDSYIPTPVR.A 1273 1276
1287
991.4840 1980.9534 1980.9536 -0.10
0
104 4.8e-008
1
1343 1054.0400 2106.0654 2106.0667 -0.59
1
52
0.0054
1
R.ATDKDLLMPIEDVFSISGR.G 1344
1348 1062.0380 2122.0614 2122.0616 -0.08
1
(51)
0.0084
1
R.ATDKDLLMPIEDVFSISGR.G 1346 1347 1349
1414 1190.0900 2378.1654 2378.1642
0.54
0
60
0.0012
1
R.ELLNEYNFPGDDTPIVSGSALK.A 1410 1411
1437 1260.6020 2519.1894 2519.1884
0.42
0
51
0.0071
1
R.TTDVTGSITLPEGTEMVMPGDNVR.I 1436
1440 1268.5990 2535.1834 2535.1833
0.06
0
(42)
0.061
1
R.TTDVTGSITLPEGTEMVMPGDNVR.I 1439 1441
1446
851.4000 2551.1782 2551.1782 -0.02
0
(44)
0.039
1
1462
898.1456 2691.4150 2691.4154 -0.14
0
(26)
1.4
1
K.VGDTIEIVGIKPTQTTTVTGVEMFR.K 1463
1465
903.4766 2707.4080 2707.4103 -0.85
0
38
0.11
1
1474
940.8427 2819.5063 2819.5103 -1.43
1
(26)
1.1
1
K.VGDTIEIVGIKPTQTTTVTGVEMFRK.E 1475 1477
1483
946.1756 2835.5050 2835.5052 -0.09
1
29
0.63
1
K.VGDTIEIVGIKPTQTTTVTGVEMFRK.E 1481
2.
gi|171855138
Mass: 39114
Score: 760
Queries matched: 21
emPAI: 0.77
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
12/9/2009
9
32
322
327
355
378
391
397
402
431
445
465
613
704
771
803
934
1012
1036
1135
353.6976
374.7211
508.7797
511.2634
525.7697
533.7670
541.7645
543.3315
544.7952
554.7794
559.7790
567.7585
629.3256
671.3251
707.4063
717.3666
785.9195
826.9100
844.4381
890.9577
705.3806
747.4276
1015.5448
1020.5122
1049.5248
1065.5194
1081.5144
1084.6484
1087.5758
1107.5442
1117.5434
1133.5024
1256.6366
1340.6356
1412.7980
1432.7186
1569.8244
1651.8054
1686.8616
1779.9008
705.3810
747.4279
1015.5451
1020.5128
1049.5249
1065.5198
1081.5148
1084.6492
1087.5761
1107.5448
1117.5437
1133.5023
1256.6361
1340.6361
1412.7987
1432.7198
1569.8249
1651.8053
1686.8611
1779.9003
-0.43
-0.34
-0.21
-0.50
-0.07
-0.36
-0.28
-0.68
-0.21
-0.50
-0.25
0.17
0.45
-0.32
-0.48
-0.81
-0.31
0.08
0.35
0.33
0
0
0
0
0
0
0
1
0
1
0
0
0
0
0
1
1
0
0
1
25
3.8
20
10
39
0.29
42
0.092
(22)
7.7
31
0.76
(20)
8.6
22
3.1
27
1.7
33
0.58
27
2.6
30
0.55
55
0.0038
58
0.0015
79 1.1e-005
47
0.033
47
0.034
71
8e-005
64
0.0004
44
0.049
1
1
1
5
7
1
2
2
1
1
2
1
1
1
1
1
1
1
1
1
Page 3 of 18
K.AAEFIR.N
K.EFIALR.A
R.NPQFQALAK.D
R.FELGEGLEK.K
-.MEITAQMVK.E
-.MEITAQMVK.E
-.MEITAQMVK.E
K.ELGGKIEIVK.Y
K.TIEQVIEEK.S
K.DLDKDFVEK.E
R.CQIGEAELAK.A
K.ALGEANGDMEK.A
K.DTTAHIQSSGIK.T
R.FYADNTVLDQR.L
K.TQIATIGENLVVR.R
K.KVDDFAAEVAAQIG.K.AIEEELKAEGKPEK.I 933
K.ATISEINSETDFVAR.N
R.VGVLIGAACESAEVANK.A
K.KATISEINSETDFVAR.N
3.
gi|157164029
Mass: 49918
Score: 728
Queries matched: 22
emPAI: 1.02
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
37
380.2343
758.4540
758.4538
0.33
0
46
0.05
1
K.TDLAVIK.I
167
451.2661
900.5176
900.5181 -0.53
0
22
13
1
K.TLVWINR.G
244
485.7166
969.4186
969.4192 -0.57
0
24
1
1
K.AEDFGFER.G
249
487.2685
972.5224
972.5240 -1.60
0
28
3.4
1
R.GDLVISANGK.A
319
508.3018 1014.5890 1014.5896 -0.53
0
54
0.0022
1
R.GGIMQGLVIK.338
516.2995 1030.5844 1030.5845 -0.05
0
(43)
0.055
1
R.GGIMQGLVIK.369
531.3138 1060.6130 1060.6128
0.21
0
24
2.1
1
K.LSVVNISTTK.T
458
565.3199 1128.6252 1128.6251
0.12
1
44
0.05
1
K.RGDLVISANGK.A
696
665.3074 1328.6002 1328.5997
0.42
1
21
5.7
1
K.SGSKAEDFGFER.G
699
667.8875 1333.7604 1333.7605 -0.06
0
82 2.5e-006
1
R.GYLVGINSAILSK.S
827
728.8638 1455.7130 1455.7140 -0.65
1
26
2.3
1
K.LVNMDHNAKDATK.S
839
736.4039 1470.7932 1470.7930
0.17
0
42
0.057
1
K.ISPDTQGVLVTDVK.S
1009
825.9203 1649.8260 1649.8260
0.01
0
78 2.6e-005
1
K.IEANGLNAITFADSSK.L 1010
1015
831.9171 1661.8196 1661.8195
0.07
0
61
0.00097
1
1026
839.9143 1677.8140 1677.8145 -0.24
0
(46)
0.029
1
1118
588.3240 1761.9502 1761.9513 -0.63
1
46
0.018
1
K.YKISPDTQGVLVTDVK.S 1119 1120
1283
986.4875 1970.9604 1970.9619 -0.73
0
86 2.7e-006
1
1295
994.4854 1986.9562 1986.9568 -0.28
0
(65) 0.00034
1
1306 1007.0000 2011.9854 2011.9851
0.18
0
44
0.045
1
K.NFIGSLTPNSSVDITYER.S
4.
gi|157165109
Mass: 35585
Score: 668
Queries matched: 22
emPAI: 1.43
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
3
350.2291
698.4436
698.4439 -0.40
0
45
0.044
1
K.AGVVVVR.D
224
478.7363
955.4580
955.4585 -0.52
0
21
4.9
1
K.YYMNPLR.K
276
494.2762
986.5378
986.5396 -1.80
0
26
5.1
1
R.INELLNSGK.A
298
501.3249 1000.6352 1000.6355 -0.20
0
(52) 0.00024
1
R.VLLMLALTK.T 297
324
509.3223 1016.6300 1016.6304 -0.32
0
57 9.4e-005
1
R.VLLMLALTK.T
342
517.7690 1033.5234 1033.5233
0.16
0
36
0.38
1
K.IQEFFLTH.357
527.3185 1052.6224 1052.6230 -0.50
0
51
0.0017
1
K.LIAAVPDINK.I
393
542.2696 1082.5246 1082.5244
0.20
0
34
0.27
1
K.TNTTGVDTFK.S
415
549.3027 1096.5908 1096.5917 -0.78
0
28
1.4
1
K.IGTVFYGNVK.Y
657
644.3579 1286.7012 1286.7017 -0.32
0
24
4.6
1
K.SDKPVVLVGAMR.N
814
721.4101 1440.8056 1440.8048
0.57
1
41
0.051
1
K.LANRINELLNSGK.A
836
735.8647 1469.7148 1469.7150 -0.13
0
37
0.22
1
K.YGFLTSDNLNAQK.A
986
813.3511 1624.6876 1624.6887 -0.62
0
32
0.19
1
R.VGSGETTMNGEVDDAK.Y
1013
827.4078 1652.8010 1652.8006
0.27
1
78 1.6e-005
1
1362 1081.5120 2161.0094 2161.0110 -0.70
0
52
0.0057
1
1366 1089.5100 2177.0054 2177.0059 -0.20
0
(27)
1.7
1
1385
744.0425 2229.1057 2229.1066 -0.42
0
33
0.52
1
R.VDIIYSHANDNPDFVNVAVK.N 1386
1395
763.4012 2287.1818 2287.1808
0.42
0
31
0.79
1
1494 1021.1400 3060.3982 3060.3982 -0.02
1
(27)
1.3
1
1495 1026.4710 3076.3912 3076.3931 -0.64
1
42
0.042
1
5.
gi|157165273
Mass: 40059
Score:
Query Observed
Mr(expt)
Mr(calc)
248 486.7554
971.4962
971.4964
253 487.7793
973.5440
973.5444
287 498.2634
994.5122
994.5124
452 563.7838 1125.5530 1125.5529
470 571.7810 1141.5474 1141.5478
480 576.3082 1150.6018 1150.6022
509 586.3397 1170.6648 1170.6648
554 609.8273 1217.6400 1217.6404
572 411.8993 1232.6761 1232.6765
675 653.3379 1304.6612 1304.6612
693 661.3449 1320.6752 1320.6747
745 692.8102 1383.6058 1383.6064
654
Queries matched: 27
emPAI: 0.89
Expect Rank
ppm Miss Score
-0.12
0
36
0.18
1
-0.36
1
44
0.057
1
-0.13
0
28
2.4
1
0.17
0
(49)
0.018
1
-0.29
0
59
0.0011
1
-0.35
0
36
0.39
1
0.01
0
25
3
1
-0.29
0
46
0.043
1
-0.31
1
21
9.5
1
0.04
1
52
0.0097
1
0.38
1
43
0.077
1
-0.39
0
69 8.6e-005
1
Peptide
K.ISDYAYIK.T
K.SVDDIKGLK.I
K.VFDPYNIK.I 288
K.SPFAMLDFAK.S 451
K.SPFAMLDFAK.S
R.IPGFGGEIYAK.L
K.SFPPEVIAALK.K
K.AIFEAAAAEVAR.D
R.IEIRIDYPSK.H
K.NAYEKLPDDLK.A
K.MKAEYPDIQVK.S
R.AGNTGMQMGGWFK.K
12/9/2009
773
780
802
935
1132
1239
708.8047
474.2694
717.3402
787.3904
889.8986
960.4827
1415.5948
1419.7864
1432.6658
1572.7662
1777.7826
1918.9508
1415.5962
1419.7874
1432.6663
1572.7671
1777.7835
1918.9523
-0.97
-0.73
-0.32
-0.54
-0.48
-0.78
0
1
0
1
0
1
(34)
40
44
44
49
20
0.13
0.064
0.032
0.055
0.0071
12
1
1
1
1
1
1
Page 4 of 18
R.AGNTGMQMGGWFK.K 774
K.IRIPGFGGEIYAK.L 781 782 783
K.VFYANVEYWDK.M 801
K.ISDYAYIKTNEEK.K.SGQYDIGYTASYYYK.G 1133 1134
K.ATNELLDEESAKDPLFK.E
6.
gi|157165374
Mass: 33156
Score: 632
Queries matched: 15
emPAI: 1.36
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
19
359.2289
716.4432
716.4432
0.02
0
27
3.3
5
K.AVLVTSK.D
108
426.2343
850.4540
850.4549 -0.96
1
25
4.6
1
K.AKGFDVSK.L
492
582.7859 1163.5572 1163.5571
0.14
1
50
0.012
1
K.FDGAKDENLR.S
746
692.8331 1383.6516 1383.6518 -0.11
0
95 3.4e-007
1
K.DSGLTSDLSSFAGK.K
894
756.8806 1511.7466 1511.7468 -0.07
1
24
4.2
1
K.DSGLTSDLSSFAGKK.Y
1022
838.3994 1674.7842 1674.7850 -0.42
0
54
0.0038
1
K.GSYDGVDHDVNTIGVK.A 1023
1091
860.0087 1718.0028 1718.0018
0.59
0
42
0.0082
1
1252
970.4805 1938.9464 1938.9469 -0.25
0
77 2.1e-005
1
K.CSVQSTGGSVYNVNNVLK.K
1298
663.3545 1987.0417 1987.0414
0.12
1
27
2
1
K.AILDNFDEYKTLHPALK.S
1304 1005.9920 2009.9694 2009.9694
0.01
0
88 1.9e-006
1
1332 1034.5280 2067.0414 2067.0419 -0.21
1
60
0.0012
1
K.CSVQSTGGSVYNVNNVLKK.E 1333
1354
713.6944 2138.0614 2138.0644 -1.41
1
34
0.47
1
1369
731.3961 2191.1665 2191.1637
1.27
1
27
1.3
1
K.KEDLVQGLSAPLHPAAEAAFK.E
7.
gi|157163977
Mass: 36762
Score: 590
Queries matched: 20
emPAI: 1.17
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score Expect Rank Peptide
42 385.1974
768.3802
768.3806 -0.50
0
49
0.0025
1
K.AADFFAK.R
48 389.2469
776.4792
776.4796 -0.45
1
30
0.79
5
K.KLEIFK.I
91 421.2263
840.4380
840.4382 -0.13
0
23
2.5
1
K.FYDVIGK.E
117 429.7501
857.4856
857.4858 -0.19
0
23
7.8
6
K.VLEEQIK.A
211 473.7511
945.4876
945.4879 -0.30
1
54
0.0055
1
K.RAEELSGGK.I 212
457 564.3136 1126.6126 1126.6135 -0.75
0
53
0.0052
1
K.FAHVVAASTPK.G 456
471 572.7957 1143.5768 1143.5772 -0.28
0
41
0.075
1
K.VQDGAVGEELK.G
582 619.8041 1237.5936 1237.5938 -0.16
0
64 0.00039
1
K.QALSEATAYER.E
708 675.3477 1348.6808 1348.6809 -0.04
0
(38)
0.2
1
K.LGNVQMAAPSFSK.F
727 683.3451 1364.6756 1364.6758 -0.13
0
60
0.0015
1
K.LGNVQMAAPSFSK.F
825 727.4004 1452.7862 1452.7864 -0.11
1
55
0.0017
1
K.FYDVIGKELIEK.T 823 824
864 744.8616 1487.7086 1487.7085
0.08
0
51
0.0072
1
K.GFVALDYWDAGFK.H
961 801.9099 1601.8052 1601.8049
0.19
0
20
11
1
K.VQVFPSAQLLDDDR.V
1003 821.9540 1641.8934 1641.8938 -0.19
1
65 0.00026
1
K.VQDGAVGEELKGLVTK.K 1004 1005
8.
gi|27807517
Mass: 42533
Score: 576
Queries matched: 26
emPAI: 0.82
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
23
362.6973
723.3800
723.3803 -0.32
0
26
1.9
1
K.ALTYEK.F
173
454.7451
907.4756
907.4763 -0.74
0
43
0.082
2
R.DTQYLLR.T 174
317
507.7192 1013.4238 1013.4236
0.20
0
(50)
0.003
1
R.ADFSGMSSGR.G
334
515.7162 1029.4178 1029.4186 -0.70
0
76 3.2e-006
1
R.ADFSGMSSGR.G 335
373
532.7957 1063.5768 1063.5774 -0.54
1
30
1.1
1
R.RDTQYLLR.T
413
549.2903 1096.5660 1096.5665 -0.44
0
24
3.8
1
K.HINTWVAEK.T 414
425
554.2711 1106.5276 1106.5284 -0.70
0
37
0.21
1
K.TYDFLSSFK.D
433
555.2537 1108.4928 1108.4938 -0.84
0
43
0.034
1
K.GNWDTQFNK.E
517
590.3602 1178.7058 1178.7063 -0.40
0
49
0.0022
1
R.LVLVNAIYFK.G 518 519
534
600.7915 1199.5684 1199.5683
0.11
0
21
7.3
1
K.EHTEERPFR.V
711
675.8489 1349.6832 1349.6835 -0.22
0
25
4.7
1
K.NVEKPVQMMFK.K
797
714.3690 1426.7234 1426.7238 -0.27
0
(35)
0.36
1
R.DLLPANSVNPMTR.L
815
722.3669 1442.7192 1442.7188
0.35
0
41
0.09
1
R.DLLPANSVNPMTR.L
997
818.8926 1635.7706 1635.7709 -0.13
0
68
0.00018
1
K.GNTAAQMCQTLSLNK.S
1050
566.3100 1695.9082 1695.9090 -0.49
1
43
0.038
1
K.IRDLLPANSVNPMTR.L 1048 1049
1074
571.6412 1711.9018 1711.9039 -1.25
1
(35)
0.25
1
K.IRDLLPANSVNPMTR.L
1383 1115.5290 2229.0434 2229.0410
1.08
0
51
0.0065
1
K.SSGGGEDVHQGFQNLLSEVNR.R 1382 1384
9.
gi|194676990
Mass: 45494
Score:
PREDICTED: similar to endopin 2B [Bos
Query Observed
Mr(expt)
Mr(calc)
16 357.6982
713.3818
713.3820
89 417.7048
833.3950
833.3953
151 439.7526
877.4906
877.4909
191 465.7662
929.5178
929.5182
275 493.7975
985.5804
985.5808
286 497.8057
993.5968
993.5971
332 513.8007 1025.5868 1025.5869
389 540.2957 1078.5768 1078.5771
442 557.8450 1113.6754 1113.6757
551 608.3035 1214.5924 1214.5931
1121 884.4123 1766.8100 1766.8112
1421 811.6998 2432.0776 2432.0781
1433 831.3996 2491.1770 2491.1754
536
Queries matched: 18
emPAI: 0.52
taurus]
-0.24
0
27
1.2
1
R.NSLQPR.H 15
-0.28
0
35
0.36
1
K.MQDLEAK.L
-0.28
0
40
0.11
1
K.LINEYVK.N
-0.37
0
41
0.16
1
K.LTPETLTR.W
-0.34
0
42
0.077
1
R.DILSQLGIK.K
-0.26
0
49
0.0035
1
K.LAVSQVIHK.A
-0.06
1
29
0.97
3
K.QLALKDPNK.N
-0.22
0
63 0.00051
1
R.HIDELSLPR.F 387 388
-0.26
1
28
0.63
1
R.DILSQLGIKK.I
-0.57
0
53
0.004
1
R.FSISSDYQLR.D 552
-0.63
0
66 0.00023
1
-0.23
0
29
0.68
1
K.TPFNPNHTYESEFHVSQNER.V 1422
0.62
1
35
0.31
1
12/9/2009
10.
gi|62126072
Mass: 46687
Query Observed
Mr(expt)
16 357.6982
713.3818
89 417.7048
833.3950
99 424.2298
846.4450
151 439.7526
877.4906
191 465.7662
929.5178
286 497.8057
993.5968
332 513.8007 1025.5868
385 537.8115 1073.6084
389 540.2957 1078.5768
405 364.2084 1089.6034
406 364.2084 1089.6034
467 568.3213 1134.6280
551 608.3035 1214.5924
1121 884.4123 1766.8100
Score: 510
Mr(calc)
713.3820
833.3953
846.4456
877.4909
929.5182
993.5971
1025.5869
1073.6089
1078.5771
1089.6039
1089.6039
1134.6285
1214.5931
1766.8112
Queries matched: 19
Page 5 of 18
emPAI: 0.41
-0.24
0
27
1.2
1
-0.28
0
35
0.36
1
-0.60
0
32
1.2
1
-0.28
0
40
0.11
1
-0.37
0
41
0.16
1
-0.26
0
49
0.0035
1
-0.06
1
29
0.97
3
-0.45
1
(22)
5.8
1
-0.22
0
63 0.00051
1
-0.44
1
36
0.21
1
-0.44
1
(29)
0.92
1
-0.37
0
40
0.074
1
-0.57
0
53
0.004
1
-0.63
0
66 0.00023
1
Peptide
R.NSLQPR.H 15
K.MQDLEAK.L
K.VPMMTLR.L
K.LINEYVK.N
K.LTPETLTR.W
K.LAVSQVIHK.A
K.QLALKDPNK.N
R.VKVPMMTLR.L 384
R.VKVPMMTLR.L
R.VKVPMMTLR.L
K.ETQSIIFLGK.V
R.FSISSDYQLR.D 552
11.
gi|157165649
Mass: 40295
Score: 506
Queries matched: 18
emPAI: 0.61
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
2 350.2110
698.4074
698.4075 -0.10
0
25
4.2
1
R.SVGIPAR.E
46 385.7076
769.4006
769.4010 -0.48
0
27
0.95
1
R.AEFYLK.G
155 442.2190
882.4234
882.4236 -0.14
0
29
1.3
3
R.NFDVNFK.H
245 485.7186
969.4226
969.4226
0.07
0
42
0.035
1
R.FSDQMGSAK.D
271 493.7159
985.4172
985.4175 -0.25
0
(23)
1.6
1
R.FSDQMGSAK.D
440 557.8447 1113.6748 1113.6757 -0.80
1
50
0.0037
1
K.AILESGKLVGK.C
537 602.3140 1202.6134 1202.6143 -0.67
1
25
3
3
R.LGEKLTNDDAK.I
700 668.3139 1334.6132 1334.6136 -0.30
0
39
0.11
2
R.DNSILGCGTGDVK.A
821 727.3663 1452.7180 1452.7184 -0.21
0
48
0.016
1
K.AIYTWVANTMQR.D 822
835 735.3638 1468.7130 1468.7133 -0.15
0
(22)
5.2
1
K.AIYTWVANTMQR.D
851 740.8455 1479.6764 1479.6769 -0.32
0
72 4.5e-005
1
K.EFSYDYVSTELK.898 759.3854 1516.7562 1516.7562
0.03
0
49
0.014
1
K.GYGWIPVDPADVTK.V 899
905 764.9201 1527.8256 1527.8256
0.01
1
33
0.41
1
K.ALEIIGNTKGDLER.A 906
910 770.8658 1539.7170 1539.7174 -0.23
0
65
0.00022
1
K.CTDINSVFVGLCR.S
928 777.8734 1553.7322 1553.7330 -0.51
0
(57)
0.002
1
K.CTDINSVFVGLCR.S
12.
gi|28317
Mass: 59492
Score: 494
Query Observed
Mr(expt)
Mr(calc)
67 404.2032
806.3918
806.3923
339 516.3026 1030.5906 1030.5910
374 532.8088 1063.6030 1063.6026
403 545.7692 1089.5238 1089.5237
494 583.2953 1164.5760 1164.5775
573 617.8432 1233.6718 1233.6717
621 631.8023 1261.5900 1261.5899
741 691.3276 1380.6406 1380.6408
751 695.8443 1389.6740 1389.6736
868 747.3710 1492.7274 1492.7270
1066 854.3900 1706.7654 1706.7649
Queries matched: 11
emPAI: 0.38
ppm Miss Score
Expect Rank
-0.51
0
30
1.2
1
-0.36
0
47
0.01
1
0.45
1
32
0.49
1
0.15
0
55
0.0037
1
-1.22
0
29
1.7
1
0.13
1
32
0.64
1
0.15
0
48
0.014
1
-0.14
0
59
0.0016
1
0.36
0
80 9.9e-006
1
0.32
1
38
0.17
1
0.32
0
44
0.026
1
Peptide
R.LAADDFR.L
R.VLDELTLTK.A
R.LASYLDKVR.A
K.VTMQNLNDR.L
R.LENEIQTYR.S
R.LKYENEVALR.Q
R.SLLEGEGSSGGGGR.G
R.ALEESNYELEGK.I
K.QSLEASLAETEGR.Y
R.SQYEQLAEQNRK.D
Mass: 58792
Score: 494
Queries matched: 11
gi|21961605
gi|114667509
Mass: 62761
Score: 494
Queries matched: 11
Mass: 58244
Score: 494
Queries matched: 11
gi|114667511
gi|114667513
Mass: 56692
Score: 494
Queries matched: 11
gi|119581085
Mass: 63308
Score: 494
Queries matched: 11
gi|195972866
Mass: 58766
Score: 494
Queries matched: 11
Mass: 58818
Score: 494
Queries matched: 11
gi|251757513
Mass: 100282
Score: 492
Queries matched: 11
gi|114667507
13.
gi|38683423
Mass: 46972
Query Observed
Mr(expt)
16 357.6982
713.3818
89 417.7048
833.3950
151 439.7526
877.4906
191 465.7662
929.5178
275 493.7975
985.5804
301 502.3056 1002.5966
332 513.8007 1025.5868
442 557.8450 1113.6754
467 568.3213 1134.6280
Score: 457
Mr(calc)
713.3820
833.3953
877.4909
929.5182
985.5808
1002.5974
1025.5869
1113.6757
1134.6285
Queries matched: 15
ppm Miss Score
-0.24
0
27
-0.28
0
35
-0.28
0
40
-0.37
0
41
-0.34
0
42
-0.79
0
33
-0.06
1
29
-0.26
1
28
-0.37
0
40
emPAI: 0.40
Expect Rank
1.2
1
0.36
1
0.11
1
0.16
1
0.077
1
0.18
1
0.97
3
0.63
1
0.074
1
Peptide
R.NSLQPR.L 15
K.MQDLEAK.L
K.LINEYVK.N
K.LTPETLTR.W
K.DILSQLGIK.K
K.LAVSHVIHK.A
K.QLALKDPNK.N
K.DILSQLGIKK.I
K.ETQSIIFLGK.V
12/9/2009
1124
1421
1433
884.9034
811.6998
831.3996
1767.7922
2432.0776
2491.1770
1767.7952
2432.0781
2491.1754
-1.66
-0.23
0.62
0
0
1
79
29
35
8.8e-006
0.68
0.31
1
1
1
Page 6 of 18
K.IFTSDADFSGITDDHK.L 1125
14.
gi|157164610
Mass: 46159
Score: 425
Queries matched: 11
emPAI: 0.41
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
52 395.2306
788.4466
788.4466
0.07
0
23
1.9
1
K.IMLGDLK.V
132 433.7419
865.4692
865.4698 -0.62
0
23
0.83
1
R.SFQFPLK.V
284 497.7365
993.4584
993.4590 -0.51
0
34
0.5
1
R.VYNMPDGAK.Y
536 601.3304 1200.6462 1200.6463 -0.01
0
70
0.00013
1
K.ELGGQSSLGVVR.L
594 623.3632 1244.7118 1244.7129 -0.83
0
33
0.31
1
K.TIVSGGLNIFPK.W 595
684 657.3405 1312.6664 1312.6663
0.09
0
49
0.012
1
R.FSSDGQSVVFIK.E
875 747.8849 1493.7552 1493.7548
0.31
0
66
0.00032
1
R.YALEGSMGSPLTLR.V
877 749.3640 1496.7134 1496.7147 -0.85
0
55
0.0047
1
K.DQPDIFIYNTGSK.N
1286 990.4584 1978.9022 1978.9021
0.09
0
71 6.6e-005
1
K.NNNSVSTFENYVVYSSR.E 1285
15.
gi|121531628
SERPINA3-4 [Bos
Query Observed
16 357.6982
49 389.7206
89 417.7048
220 476.2605
316 506.7947
332 513.8007
448 561.3133
500 584.8459
527 596.8323
707 673.8691
1356 714.6884
Mass: 46282
taurus]
Mr(expt)
713.3818
777.4266
833.3950
950.5064
1011.5748
1025.5868
1120.6120
1167.6772
1191.6500
1345.7236
2141.0434
Score: 402
Mr(calc)
713.3820
777.4272
833.3953
950.5073
1011.5753
1025.5869
1120.6128
1167.6764
1191.6499
1345.7241
2141.0429
emPAI: 0.23
-0.24
0
27
1.2
1
-0.75
0
24
3.2
1
-0.28
0
35
0.36
1
-0.87
0
44
0.052
1
-0.43
0
34
0.35
1
-0.06
1
29
0.97
3
-0.69
0
55
0.0031
1
0.73
1
28
0.3
1
0.11
1
35
0.36
1
-0.36
1
64 0.00046
1
0.22
1
27
2.3
1
gi|226373739
Mass: 46361
Score: 402
Mass: 59194
Score:
membrane glycoprotein
Mr(expt)
Mr(calc)
732.4528
732.4534
805.4076
805.4083
885.4414
885.4419
901.4366
901.4368
1159.5652 1159.5656
1173.5814 1173.5812
1244.5646 1244.5648
1250.5420 1250.5424
1264.5578 1264.5580
1283.5348 1283.5340
1355.6112 1355.6115
1728.7084 1728.7083
2411.0089 2411.0084
Queries matched: 14
Peptide
R.NSLQPR.R 15
K.IEELFK.D
K.MQDLEAK.L
K.SLINDYVK.N
R.IHELYLPK.F
K.QLALKDPNK.N
K.DTQSIIFLGK.V 449
R.RIHELYLPK.F
K.TQGKIEELFK.D
K.IEELFKDLSPR.T 706
R.VLYSSEAFPTNFRDPEAAK.S
Queries matched: 14
16.
gi|115495209
zymogen granule
Query Observed
28 367.2337
65 403.7111
156 443.7280
168 451.7256
485 580.7899
511 587.7980
593 623.2896
607 626.2783
624 633.2862
652 642.7747
718 678.8129
1099 865.3615
1419 804.6769
378
Queries matched: 14
emPAI: 0.31
2 [Bos taurus]
-0.75
0
46 0.0086
1
R.FNLVLK.N
-0.78
0
24
3.7
2
R.FVGDGGVR.M 66
-0.47
0
46 0.0099
1
R.FSVQMFK.F
-0.15
0
(33)
0.25
1
R.FSVQMFK.F
-0.26
0
(25)
3.8
1
R.LEGTPQCSLR.Y
0.22
0
36
0.35
1
R.LEGTPQCSLR.Y
-0.14
0
23
3
1
K.ACPGPYYVYR.L
-0.27
0
(28)
1
1
R.MPEDCVPTFR.C
-0.14
0
38
0.11
1
R.MPEDCVPTFR.C
0.69
0
29
0.39
1
R.YCTDPATAEDK.C
-0.17
0
52 0.0043
1
R.LVSGTWGCFCR.Q
0.08
1
39
0.024
1
R.YCTDPATAEDKCDR.T
0.19
1
45
0.006
1
R.STENTEDIQGCDSDKHGWYR.F
17.
gi|126165236
Mass: 46368
Score: 353
Queries matched: 12
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
16 357.6982
713.3818
713.3820 -0.24
0
27
1.2
1
R.NSLQPR.R 15
49 389.7206
777.4266
777.4272 -0.75
0
24
3.2
1
K.IEELFK.Y
89 417.7048
833.3950
833.3953 -0.28
0
35
0.36
1
K.MQDLEAK.L
191 465.7662
929.5178
929.5182 -0.37
0
41
0.16
1
K.LTPETLTR.W
220 476.2605
950.5064
950.5073 -0.87
0
44
0.052
1
R.SLINDYVK.N
316 506.7947 1011.5748 1011.5753 -0.43
0
34
0.35
1
R.IHELYLPK.F
448 561.3133 1120.6120 1120.6128 -0.69
0
55 0.0031
1
K.DTQSIIFLGK.V 449
500 584.8459 1167.6772 1167.6764
0.73
1
28
0.3
1
R.RIHELYLPK.F
527 596.8323 1191.6500 1191.6499
0.11
1
35
0.36
1
K.TQGKIEELFK.Y
683 656.8174 1311.6202 1311.6208 -0.39
0
31
0.84
1
K.HTEQAEFHVSK.N
18.
gi|11935049
Mass: 66027
Query Observed
Mr(expt)
250 487.2690
972.5234
341 517.2616 1032.5086
375 533.2638 1064.5130
672 651.8610 1301.7074
754 697.3695 1392.7244
846 738.3957 1474.7768
998 819.9339 1637.8532
1087 858.9288 1715.8430
Score: 347
Mr(calc)
972.5240
1032.5087
1064.5138
1301.7078
1392.7249
1474.7780
1637.8525
1715.8438
Queries matched: 8
emPAI: 0.21
ppm Miss Score
Expect Rank
-0.54
0
40
0.22
1
-0.08
0
28
1.6
1
-0.71
0
41
0.12
1
-0.29
0
74 4.5e-005
1
-0.30
1
43
0.053
1
-0.77
0
33
0.66
1
0.43
1
27
1.7
1
-0.44
0
61
0.00083
1
Peptide
K.IEISELNR.V
R.TLLEGEESR.M
K.AQYEDIAQK.S
R.SLDLDSIIAEVK.A
R.TNAENEFVTIKK.D
R.FLEQQNQVLQTK.W
K.SLNNQFASFIDKVR.F
K.QISNLQQSISDAEQR.G
12/9/2009
gi|119395750
Mass: 65999
gi|160961491
Mass: 65450
Score: 347
Queries matched: 8
Score: 347
Queries matched: 8
19.
gi|158605017
Mass: 30046
Score: 340
Queries matched: 12
emPAI: 0.23
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
59 398.2034
794.3922
794.3923 -0.05
0
28
2.2
1
K.GVTFTDR.G
68 404.7316
807.4486
807.4490 -0.48
1
25
3.9
1
K.SIADKFK.G
182 460.2220
918.4294
918.4294
0.04
0
27
1.4
1
K.TDIENAEK.F
306 504.2739 1006.5332 1006.5335 -0.24
1
38
0.22
1
K.VEKEFVEK.G
685 657.8384 1313.6622 1313.6615
0.55
1
37
0.24
1
R.GTYIKYEANQK.G
722 679.3590 1356.7034 1356.7038 -0.25
1
28
2.2
1
K.GVTFTDRGTYIK.Y 723
953 799.8969 1597.7792 1597.7810 -1.11
0
(47)
0.023
1
R.NAVMYNDFVVIADK.S
956 800.9381 1599.8616 1599.8620 -0.25
0
37
0.21
1
K.LLDKPLFTPDANTR.K
976 807.8951 1613.7756 1613.7759 -0.18
0
95 3.2e-007
1
R.NAVMYNDFVVIADK.S
1158 907.9399 1813.8652 1813.8669 -0.90
0
26
2.8
1
K.LGEDCNADILFVHSPK.V 1159
20.
gi|157164497
Mass: 29815
Score: 335
Queries matched: 10
emPAI: 0.70
Query Observed
Mr(expt)
Mr(calc)
398 543.7817 1085.5488 1085.5505 -1.56
1
34
0.34
1
K.GYTEGYLRK.S 399
475 573.8112 1145.6078 1145.6081 -0.19
0
28
3.1
1
K.SVVAEPLFER.K
619 630.3396 1258.6646 1258.6656 -0.76
0
60
0.0011
1
K.LEDELLELASK.T 620
808 719.8738 1437.7330 1437.7324
0.42
0
47
0.03
1
K.NTTNNTPAVIHTR.I
838 736.3912 1470.7678 1470.7679 -0.00
0
62 0.00057
1
983 811.4365 1620.8584 1620.8610 -1.57
1
39
0.15
1
R.YAKLEDELLELASK.T 984
995 817.9332 1633.8518 1633.8523 -0.25
1
67 0.00028
1
K.AQTTESSSLGKDILGK.I
21.
gi|77736341
Mass: 52315
serpin peptidase inhibitor,
Query Observed
Mr(expt)
1 350.1886
698.3626
175 456.2690
910.5234
236 481.7486
961.4826
481 577.8354 1153.6562
647 641.3091 1280.6036
966 801.9155 1601.8164
1214 939.9427 1877.8708
1224 947.9420 1893.8694
Page 7 of 18
Score: 317
Queries matched: 13
emPAI: 0.28
clade C (antithrombin), member 1 [Bos taurus]
Mr(calc)
698.3639 -1.80
0
22
4.1
1
K.ELFYK.A
910.5236 -0.17
0
32
0.4
1
R.LPGIVAEGR.S
961.4828 -0.21
0
68 0.00019
1
K.SSELVSANR.L
1153.6567 -0.42
1
39
0.082
1
K.SRLPGIVAEGR.S 482
1280.6037 -0.05
0
36
0.68
1
R.SDLYVSDAFHK.A 644 645 646
1601.8161
0.19
1
49
0.013
1
K.LQPLDFKGNAEQSR.L
1877.8717 -0.44
0
72
6e-005
1
1893.8666
1.51
0
(37)
0.16
1
K.EQLQDMGLEDLFSPEK.S 1223
gi|109940161
Mass: 52314
Score: 317
gi|157838185
Mass: 48618
Score: 317
Queries matched: 13
Flags: Precursor
Queries matched: 13
22.
gi|194038347
Mass: 46611
Score:
PREDICTED: alpha-1-antichymotrypsin 3
Query Observed
Mr(expt)
Mr(calc)
20 360.1913
718.3680
718.3683
83 417.2218
832.4290
832.4299
103 425.2194
848.4242
848.4248
231 480.7791
959.5436
959.5440
273 493.7795
985.5444
985.5444
362 529.2803 1056.5460 1056.5465
443 558.8295 1115.6444 1115.6451
1041 845.4077 1688.8008 1688.8006
1364 725.3746 2173.1020 2173.1015
291
Queries matched: 10
emPAI: 0.15
[Sus scrofa]
-0.40
0
29
2
1
R.MAAVEAK.L
-1.02
0
(20)
16
1
K.VPMMSLR.G
-0.67
0
21
8.6
4
K.VPMMSLR.G
-0.36
0
49
0.024
1
R.IDALYLPR.F
0.05
0
29
1.6
1
K.QLVSLDPSK.N
-0.39
1
29
1.5
6
R.WRDSLQPR.R 363
-0.58
1
48
0.013
1
R.RIDALYLPR.F
0.15
0
56 0.0025
1
K.VFTHEADLSGVTGDNK.L
0.20
1
31
0.8
1
K.VFTHEADLSGVTGDNKLGVSK.V
23.
gi|157164740
Mass: 36430
Score: 283
Queries matched: 9
emPAI: 0.30
Query Observed
Mr(expt)
Mr(calc)
77 411.7396
821.4646
821.4647 -0.02
1
28
1.8
1
K.YAAEIKK.V
98 422.7371
843.4596
843.4603 -0.73
0
26
1.5
3
R.YHANLVK.N
170 453.2639
904.5132
904.5130
0.26
1
41
0.11
1
R.FALKAEAR.D
585 620.8376 1239.6606 1239.6612 -0.42
1
30
0.95
1
R.YQVTDRFALK.A
677 653.3560 1304.6974 1304.6976 -0.12
0
61 0.00097
1
K.AVAEVLAGYGVEK.A
1043 845.9725 1689.9304 1689.9301
0.20
0
59
0.0006
1
1199 929.9672 1857.9198 1857.9221 -1.21
0
39
0.16
1
K.VVLAGHTDSVGAEAYNQK.L 1198 1200
24.
gi|119914036
Mass: 34009
Score: 281
Queries matched: 9
emPAI: 0.20
Query
Observed
Mr(expt)
Mr(calc)
16
357.6982
713.3818
713.3820 -0.24
0
27
1.2
1
R.NSLQPR.- 15
49
389.7206
777.4266
777.4272 -0.75
0
24
3.2
1
K.IEELFK.D
89
417.7048
833.3950
833.3953 -0.28
0
35
0.36
1
K.MQDLEAK.L
220
476.2605
950.5064
950.5073 -0.87
0
44
0.052
1
K.SLINDYVK.K
527
596.8323 1191.6500 1191.6499
0.11
1
35
0.36
1
K.TQGKIEELFK.D
707
673.8691 1345.7236 1345.7241 -0.36
1
64 0.00046
1
K.IEELFKDLSPR.T 706
12/9/2009
1324
1021.9890
2041.9634
2041.9633
0.09
0
52
0.0073
1
Page 8 of 18
25.
gi|154173809
Mass: 46458
Score: 266
Queries matched: 8
emPAI: 0.32
translocation protein TolB [Campylobacter curvus 525.92]
Query Observed
Mr(expt)
Mr(calc)
52 395.2306
788.4466
788.4466
0.07
0
23
1.9
1
K.IMLGDLK.V
132 433.7419
865.4692
865.4698 -0.62
0
23
0.83
1
R.SFQFPLK.V
536 601.3304 1200.6462 1200.6463 -0.01
0
70 0.00013
1
K.ELGGQSSLGVVR.L
594 623.3632 1244.7118 1244.7129 -0.83
0
33
0.31
1
K.TIVSGGLNIFPK.W 595
684 657.3405 1312.6664 1312.6663
0.09
0
49
0.012
1
R.FSSDGQSVVFIK.E
942 790.4075 1578.8004 1578.7997
0.48
0
67 0.00057
1
K.IIDSMGMLIASDVSK.D
971 806.4020 1610.7894 1610.7895 -0.05
0
(53) 0.0067
1
K.IIDSMGMLIASDVSK.D
26.
gi|223039637
Mass: 39873
Score: 263
Queries matched: 9
emPAI: 0.61
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
46 385.7076
769.4006
769.4010 -0.48
0
27
0.95
1
R.AEFYLK.G
440 557.8447 1113.6748 1113.6757 -0.80
1
50
0.0037
1
K.AILESGKLVGK.C
691 661.3062 1320.5978 1320.5980 -0.12
0
72 4.9e-005
1
R.DNSVIGCGTGDVK.A
700 668.3139 1334.6132 1334.6136 -0.30
0
(70) 7.3e-005
1
R.DNSVIGCGTGDVK.A
821 727.3663 1452.7180 1452.7184 -0.21
0
48
0.016
1
K.AIYTWVANTMQR.D 822
835 735.3638 1468.7130 1468.7133 -0.15
0
(22)
5.2
1
K.AIYTWVANTMQR.D
910 770.8658 1539.7170 1539.7174 -0.23
0
65
0.00022
1
K.CTDINSVFVGLCR.A
928 777.8734 1553.7322 1553.7330 -0.51
0
(57)
0.002
1
K.CTDINSVFVGLCR.A
27.
gi|157164833
phosphopyruvate
Query Observed
194 466.7454
257 488.2845
530 597.8036
680 654.8541
883 752.9037
939 788.9146
1234 635.3286
28.
gi|158604943
Mass: 37938
Score: 260
Queries matched: 6
emPAI: 0.40
aspartate-semialdehyde dehydrogenase [Campylobacter concisus 13826]
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
115
429.7432
857.4718
857.4719 -0.07
0
41
0.13
1
R.VGAATNAVR.I
694
662.3842 1322.7538 1322.7558 -1.47
1
40
0.037
1
K.APSVVVVDNPAKK.E
717
677.3361 1352.6576 1352.6572
0.32
0
54
0.0038
1
R.VDVSTYQAASGAGK.E
1156
903.9421 1805.8696 1805.8697 -0.01
0
78 2.1e-005
1
K.FAADSGAVVIDNTSHFR.M 1155
1326 1022.5370 2043.0594 2043.0598 -0.18
0
47
0.018
1
R.VMEEVDFPVGELLPLASAK.S
29.
gi|157164054
Mass: 39558
Score: 255
Queries matched: 7
emPAI: 0.49
cytochrome c oxidase, heme b and copper-binding subunit, membrane-bound [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
314 506.2798 1010.5450 1010.5437
1.33
0
21
4.8
1
K.DIVSFGFVK.N
972 806.9380 1611.8614 1611.8620 -0.37
0
58
0.0012
1
K.VGILDADIYGPNIPR.M
974 807.4194 1612.8242 1612.8243 -0.03
0
65 0.00034
1
R.MLGEVNTQPQVVGNK.L
988 815.4166 1628.8186 1628.8192 -0.35
0
(47)
0.027
1
R.MLGEVNTQPQVVGNK.L
1063 852.9530 1703.8914 1703.8916 -0.10
0
54
0.0038
1
R.VMGSNEYVLNLIQPK.I
1231 949.5229 1897.0312 1897.0309
0.20
0
57
0.0014
1
K.AYNTQILAEIPIEPAVR.V 1230
30.
gi|181402
Mass: 65825
Score: 253
Query Observed
Mr(expt)
Mr(calc)
250 487.2690
972.5234
972.5240
426 554.2744 1106.5342 1106.5356
612 627.8071 1253.5996 1253.6001
697 665.3663 1328.7180 1328.7187
828 730.9030 1459.7914 1459.7922
846 738.3957 1474.7768 1474.7780
Mass: 45147
Score: 262
Queries matched: 8
emPAI: 0.24
hydratase [Campylobacter concisus 13826]
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
931.4762
931.4763 -0.06
0
26
3.7
1
R.AATEIYHK.L
974.5544
974.5549 -0.45
0
21
13
2
K.SLNIPLYR.Y
1193.5926 1193.5928 -0.12
0
42
0.075
1
K.EAGYELGSQIK.L
1307.6936 1307.6972 -2.75
0
30
1.2
1
K.LALDVASSELYK.D
1503.7928 1503.7933 -0.28
0
39
0.13
1
K.IAEAVIGLDAYNQK.A
1575.8146 1575.8145
0.12
0
71 9.4e-005
1
K.VQLVGDDLFVTNEK.I
1902.9640 1902.9647 -0.37
1
33
0.59
1
R.LSDGTEASAIVPSGASTGKR.E 1233
Queries matched: 6
emPAI: 0.10
-0.54
0
40
0.22
1
-1.22
0
40
0.081
1
-0.32
0
33
0.48
1
-0.50
0
63 0.00038
1
-0.53
0
44
0.044
1
-0.77
0
33
0.66
1
Peptide
K.IEISELNR.V
K.AQYEEIAQR.S
R.GFSSGSAVVSGGSR.R
R.NLDLDSIIAEVK.A
K.VDLLNQEIEFLK.V
R.FLEQQNQVLQTK.W
gi|47132620
Mass: 65393
Score: 253
Queries matched: 6
gi|148727309
Mass: 65944
Score: 253
Queries matched: 6
31.
gi|47523844
Mass: 47164
Score: 242
Queries matched: 5
emPAI: 0.14
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
320 508.3109 1014.6072 1014.6073 -0.08
0
36
0.17
1
K.SLLGNLGITK.V
436 555.8051 1109.5956 1109.5968 -1.07
0
35
0.19
1
K.LTISGTYDLK.S
951 799.4225 1596.8304 1596.8300
0.28
0
51
0.0065
1
R.IAPNLADFAFSLYR.Q
1269 982.4783 1962.9420 1962.9422 -0.09
0
96 2.5e-007
1
1396 764.7248 2291.1526 2291.1533 -0.30
1
23
5
1
K.VFSDEADLSGVTEEQPLKLSK.A
12/9/2009
Page 9 of 18
32.
gi|114667176
Mass: 107386
Score:
PREDICTED: similar to Keratin, type I
Query Observed
Mr(expt)
Mr(calc)
67 404.2032
806.3918
806.3923
374 532.8088 1063.6030 1063.6026
403 545.7692 1089.5238 1089.5237
483 579.2988 1156.5830 1156.5836
568 616.8022 1231.5898 1231.5906
578 618.2682 1234.5218 1234.5215
237
Queries matched: 6
emPAI: 0.09
cytoskeletal 14 (Cytokeratin-14) (CK-14) (Keratin-14) (K14) [Pan troglodytes]
-0.51
0
30
1.2
1
R.LAADDFR.T
0.45
1
32
0.49
1
R.LASYLDKVR.A
0.15
0
55 0.0037
1
K.VTMQNLNDR.L
-0.52
0
40
0.091
1
R.QGVDADINGLR.Q
-0.58
0
46
0.027
1
0.32
0
35
0.11
1
R.FSSSSGYGGGSSR.V
33.
gi|157164268
Mass: 36935
ketol-acid reductoisomerase
Query Observed
Mr(expt)
57 396.7159
791.4172
716 676.8840 1351.7534
735 684.8813 1367.7480
987 813.4285 1624.8424
1216 940.9365 1879.8584
34.
gi|157165008
transketolase A
Query Observed
55 395.7184
204 471.7572
268 491.2841
550 607.3083
946 793.9307
980 810.4443
1025 839.0011
35.
gi|157164709
Mass: 47282
Score: 226
Queries matched: 7
emPAI: 0.14
Query Observed
Mr(expt)
Mr(calc)
270 492.7952
983.5758
983.5764 -0.52
0
23
3.6
1
K.ANGIILDLR.N
333 514.3290 1026.6434 1026.6437 -0.27
0
51 0.0026
1
K.TPITITILR.K
401 544.3030 1086.5914 1086.5921 -0.57
1
29
1.3
1
K.KVDEDIQLK.T
477 574.8190 1147.6234 1147.6237 -0.24
0
39
0.16
1
R.AVIIGENTFGK.G
766 704.3683 1406.7220 1406.7227 -0.49
0
45
0.058
1
K.MIENENILYIR.V
787 712.3657 1422.7168 1422.7177 -0.56
0
(23)
6.2
2
K.MIENENILYIR.V
1129 886.4514 1770.8882 1770.8887 -0.27
0
39
0.15
1
K.DSALTVISPIEDTPADK.A
36.
gi|157163930
Mass: 45716
Score:
outer membrane protein [Campylobacter
Query Observed
Mr(expt)
Mr(calc)
262 489.7536
977.4926
977.4930
549 606.7801 1211.5456 1211.5458
636 635.8449 1269.6752 1269.6757
732 684.8306 1367.6466 1367.6469
948 795.8746 1589.7346 1589.7361
37.
gi|126165264
SERPINA3-8 [Bos
Query Observed
30 367.7258
89 417.7048
220 476.2605
448 561.3133
1130 887.4473
Score: 233
Queries matched: 5
emPAI: 0.41
[Campylobacter concisus 13826]
Mr(calc)
ppm Miss Score
Expect Rank Peptide
791.4177 -0.62
0
27
2.7
1
K.DFILER.K
1351.7533
0.09
0
63
0.00026
1
K.APANIDVIMIAPK.A
1367.7482 -0.14
0
(28)
0.96
1
K.APANIDVIMIAPK.A
1624.8420
0.25
0
61
0.00072
1
1879.8588 -0.19
0
82 4.5e-006
1
Mass: 44829
Score: 230
Queries matched: 8
emPAI: 0.15
Mr(expt)
Mr(calc)
789.4222
789.4232 -1.23
0
28
2.8
1
K.ASGVSELK.F
941.4998
941.5004 -0.61
0
35
0.91
1
K.HIVMIDSK.G
980.5536
980.5542 -0.59
0
24
2.3
1
K.EPVPAEVLK.A
1212.6020 1212.6026 -0.49
1
28
1.4
1
R.VKDFDVEAYK.A
1585.8468 1585.8464
0.29
0
42
0.057
1
K.ALAPTFGGINLEDIR.A
1618.8740 1618.8753 -0.76
0
37
0.19
1
K.GADMFLGLSKPGVVTK.E 981
1675.9876 1675.9872
0.26
1
36
0.022
1
K.ALAQLAKEPVPAEVLK.A
Mass: 46888
taurus]
Mr(expt)
733.4370
833.3950
950.5064
1120.6120
1772.8800
225
Queries matched: 6
emPAI: 0.23
concisus 13826]
-0.40
0
53
0.0082
1
K.NVDLSGFAR.Y
-0.16
0
46
0.025
1
R.NEWSLDASYK.Y
-0.38
0
63 0.00053
1
K.LTFLSWYAAAK.D 637
-0.21
1
34
0.39
1
K.RNEWSLDASYK.Y
-0.94
1
29
1
1
R.NEWSLDASYKYSK.K
Score: 219
Mr(calc)
733.4374
833.3953
950.5073
1120.6128
1772.8792
Queries matched: 6
emPAI: 0.15
-0.51
0
25
1.2
1
-0.28
0
35
0.36
1
-0.87
0
44
0.052
1
-0.69
0
55 0.0031
1
0.46
0
60
0.001
1
gi|151554250
Mass: 46935
Score: 219
Peptide
K.IVDLFK.D
K.MQDLEAK.L
K.SLINDYVK.N
K.DTQSIIFLGK.V 449
K.AVLDVGEEGTEGAAATGVK.V
Queries matched: 6
38.
gi|157164830
Mass: 38482
Score: 213
Queries matched: 9
emPAI: 0.39
Query Observed
Mr(expt)
Mr(calc)
285 497.7472
993.4798
993.4801 -0.26
0
36
0.32
1
R.SITDMQGVK.V
400 543.8055 1085.5964 1085.5968 -0.34
0
40
0.098
2
K.DLQIEELVK.L
580 619.3378 1236.6610 1236.6615 -0.36
0
55
0.0031
1
K.NGFSAVNLIFR.D
600 624.3953 1246.7760 1246.7761 -0.06
0
47 0.00087
1
K.VLHLLGGANLLK.V 597 598 599 601
1351 711.3422 2131.0048 2131.0044
0.16
1
35
0.32
1
K.NGFSAVNLIFRDYPDMEK.S
39.
gi|1942778
Mass: 15089
Chain A, Deoxy-Haemoglobin
Query Observed
Mr(expt)
343 521.2753 1040.5360
360 529.2729 1056.5312
474 573.3297 1144.6448
Score: 203
Queries matched: 8
emPAI: 1.25
Trapped In The High-Affinity (R) State
Mr(calc)
1040.5365 -0.43
0
45
0.025
1
R.MFLGFPTTK.T
1056.5314 -0.16
0
(34)
0.37
1
R.MFLGFPTTK.T 359
1144.6452 -0.28
1
57 0.0018
1
-.VLSAADKTNVK.A
12/9/2009
632
881
634.8585
750.3658
1267.7024
1498.7170
gi|146149083
Mass:
Mass:
gi|146149272
40.
1267.7024
1498.7164
0.06
0.40
15220
Score: 203
caballus]
15220
Score: 203
caballus]
gi|136429
Mass: 24394
Score: 202
Query
Observed
Mr(expt)
Mr(calc)
95
421.7581
841.5016
841.5022
347
523.2855 1044.5564 1044.5564
1376 1106.0560 2210.0974 2210.0967
1393 1142.0930 2282.1714 2282.1729
1400
767.0634 2298.1684 2298.1678
0
0
55
47
0.002
0.018
1
1
Page 10 of 18
K.FLSSVSTVLTSK.Y
K.VGGHAGEFGAEALER.M 879 880
Queries matched: 8
Queries matched: 8
Queries matched: 11
emPAI: 0.67
Expect Rank
ppm Miss Score
-0.60
0
56
0.0042
1
0.09
0
46
0.047
1
0.33
0
77 2.5e-005
1
-0.63
0
(22)
6.5
1
0.25
0
23
4.4
1
Peptide
R.VATVSLPR.S 93 94
K.LSSPATLNSR.V 346
R.LGEHNIDVLEGNEQFINAAK.I 1374 1375 1377
Mass: 23458
Score: 202
Queries matched: 11
gi|494360
gi|999627
Mass: 8814
Score: 202
Queries matched: 11
gi|1942351
Mass: 13284
Score: 202
Queries matched: 11
gi|2914482
Mass: 23460
Score: 202
Queries matched: 11
gi|3318722
Mass: 23457
Score: 202
Queries matched: 11
gi|110590762
Mass: 23458
Score: 202
Queries matched: 11
gi|157878102
Mass: 24157
Score: 202
Queries matched: 11
gi|242253868
Mass: 25865
Score: 202
Queries matched: 11
41.
gi|157164277
Mass: 36126
Score: 197
Queries matched: 5
emPAI: 0.19
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
86 417.2347
832.4548
832.4555 -0.83
0
28
0.74
1
K.VAINGFGR.I
719 678.8771 1355.7396 1355.7409 -0.89
0
55
0.003
1
R.AAALNIGPTTTGAAK.A
915 773.9016 1545.7886 1545.7899 -0.81
1
28
2
1
K.RPASKEEINEAFR.A
1014 828.4739 1654.9332 1654.9328
0.28
0
86 8.8e-007
1
1021 836.4720 1670.9294 1670.9277
1.04
0
(46)
0.014
1
42.
gi|122411
Mass: 15236
Score: 195
Queries matched: 6
emPAI: 0.83
Query Observed
Mr(expt)
Mr(calc)
343 521.2753 1040.5360 1040.5365 -0.43
0
45
0.025
1
R.MFLGFPTTK.T
360 529.2729 1056.5312 1056.5314 -0.16
0
(34)
0.37
1
R.MFLGFPTTK.T 359
474 573.3297 1144.6448 1144.6452 -0.28
1
57 0.0018
1
M.VLSAADKTNVK.A
632 634.8585 1267.7024 1267.7024
0.06
0
55
0.002
1
K.FLSSVSTVLTSK.Y
896 758.3628 1514.7110 1514.7113 -0.20
0
38
0.12
1
K.VGGHAGEYGAEALER.M
Mass: 15105
Score: 195
Queries matched: 6
gi|230479
gi|230632
Mass: 15105
Score: 195
Queries matched: 6
gi|313500
Mass: 15236
Score: 195
Queries matched: 6
gi|24158717
Mass: 15105
Score: 195
Queries matched: 6
43.
gi|47523270
Mass: 46616
Score: 195
Queries matched: 6
emPAI: 0.07
alpha-1-antichymotrypsin 2 [Sus scrofa]
Query Observed
Mr(expt)
Mr(calc)
20 360.1913
718.3680
718.3683 -0.40
0
29
2
1
R.MAAVEAK.L
30 367.7258
733.4370
733.4374 -0.51
0
25
1.2
1
K.IVDLFK.K
63 402.2168
802.4190
802.4193 -0.37
0
26
6.7
1
R.VPMMGIR.A
193 465.7717
929.5288
929.5294 -0.60
0
58 0.0025
1
R.SSNLLQLR.L
487 580.8389 1159.6632 1159.6641 -0.74
0
24
2.3
1
R.WIVELYLPK.F
614 629.3655 1256.7164 1256.7169 -0.35
0
31
0.41
1
K.VVLVNYIYFK.A
44.
gi|157165527
Mass: 43876
Score: 175
Queries matched: 4
emPAI: 0.16
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
196 467.2265
932.4384
932.4392 -0.80
0
25
4.1
1
R.FAYQYNK.K
262 489.7536
977.4926
977.4930 -0.40
0
53
0.0082
1
K.NVDLSGFAR.Y
12/9/2009
368
989
45.
531.2742
816.3726
1060.5338
1630.7306
1060.5341
1630.7304
-0.28
0.17
1
0
29
68
2.2
9.1e-005
1
1
Page 11 of 18
R.FAYQYNKK.L
K.GNFFYATSAYTFDK.F
gi|154175542
Mass: 22096
Score: 166
Queries matched: 5
emPAI: 0.33
Query Observed
Mr(expt)
Mr(calc)
328 511.2727 1020.5308 1020.5314 -0.54
0
27
1.9
1
R.FPIVADMTK.S
539 602.8431 1203.6716 1203.6724 -0.62
0
49
0.01
1
R.HAVINDLPLGR.N
679 654.3513 1306.6880 1306.6881 -0.06
1
34
0.46
1
R.GSFLLDKDGTVR.H 678
844 737.8987 1473.7828 1473.7827
0.07
0
57 0.0026
1
R.GFDVLLEDAGVALR.G
gi|157164012
Mass: 22011
Score: 166
Queries matched: 5
46.
gi|225632
Mass: 24420
casein alphaS1
Query Observed
Mr(expt)
630 634.3560 1266.6974
747 692.8685 1383.7224
1002 821.4373 1640.8600
1115 880.4762 1758.9378
Score: 164
Mr(calc)
1266.6972
1383.7227
1640.8603
1758.9377
Queries matched: 7
emPAI: 0.47
0.20
0
45
0.025
1
-0.21
0
40
0.087
1
-0.14
1
23
4.5
1
0.11
0
55 0.0021
1
Peptide
R.YLGYLEQLLR.L 629
R.FFVAPFPEVFGK.E 748
R.FFVAPFPEVFGKEK.V
K.HQGLPQEVLNENLLR.F 1116
gi|30794348
Mass: 24513
Score: 164
Queries matched: 7
Mass: 13839
Score: 164
Queries matched: 7
gi|159793187
Mass: 23598
Score: 164
Queries matched: 7
gi|159793191
gi|159793193
Mass: 18671
Score: 164
Queries matched: 7
gi|159793197
Mass: 23473
Score: 164
Queries matched: 7
gi|159793209
Mass: 22442
Score: 164
Queries matched: 7
gi|195973860
Mass: 24427
Score: 164
Queries matched: 7
47.
gi|157164634
Mass: 48581
Score: 161
Queries matched: 3
emPAI: 0.14
type II secretion system protein E [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
340 516.7949 1031.5752 1031.5764 -1.09
0
37
0.17
2
K.ADAFLVALGR.V
1007 824.9373 1647.8600 1647.8580
1.22
0
67 0.00023
1
R.LQTNVSNIYAVGDVR.G
1232 952.4572 1902.8998 1902.8999 -0.04
0
57
0.0019
1
K.EAYAPFEITNSNVFSSK.T
48.
gi|122614
Mass: 15998
Score: 159
RecName: Full=Hemoglobin subunit beta;
Query
Observed
Mr(expt)
Mr(calc)
640
637.8663 1273.7180 1273.7183
796
713.8463 1425.6780 1425.6776
1302 1000.4640 1998.9134 1998.9146
1307 1008.4620 2014.9094 2014.9095
Queries matched: 4
emPAI: 0.78
AltName: Full=Hemoglobin beta chain; AltName: Full=Beta-globin
ppm Miss Score
Expect Rank Peptide
-0.19
0
40
0.066
1
R.LLVVYPWTQR.F
0.32
0
50
0.011
1
K.DFTPELQASYQK.V
-0.57
0
(51)
0.0059
1
-0.03
0
69 8.6e-005
1
gi|230480
Mass: 15970
Score: 159
Queries matched: 4
gi|255683515
Mass: 16129
Score: 159
Queries matched: 4
49.
gi|2232299
Mass: 47885
Score: 158
Queries matched: 13
emPAI: 0.14
Query Observed
Mr(expt)
Mr(calc)
410 547.2813 1092.5480 1092.5485 -0.42
0
(26)
3.2
1
R.ESASVTCLVK.G
429 554.2891 1106.5636 1106.5641 -0.45
0
35
0.39
1
R.ESASVTCLVK.G
626 633.8088 1265.6030 1265.6040 -0.78
0
38
0.18
1
K.ALETHTYFER.H 625 627
993 817.4279 1632.8412 1632.8413 -0.02
0
48
0.018
1
K.GFAPADVFVQWLQR.G 994
1137 595.3306 1782.9700 1782.9702 -0.14
0
(31)
0.47
1
K.DVAMKPPSVYLLPPTR.E 1136
1151 900.4901 1798.9656 1798.9651
0.29
0
35
0.23
1
K.DVAMKPPSVYLLPPTR.E 1148 1150 1152
gi|3834667
Mass: 48183
Score: 158
Queries matched: 13
gi|24496448
Mass: 65621
Score: 158
Queries matched: 13
gi|33413902
Mass: 49106
Score: 158
Queries matched: 13
gi|34538498
Mass: 49909
Score: 158
Queries matched: 13
12/9/2009
50.
Page 12 of 18
gi|154175426
Mass: 28890
Score: 155
Queries matched: 3
emPAI: 0.24
Query Observed
Mr(expt)
Mr(calc)
243 485.2900
968.5654
968.5655 -0.01
0
46
0.015
1
R.VNAISAGPIK.T
430 554.2929 1106.5712 1106.5720 -0.70
0
57 0.0027
1
K.TLAASGIGDFR.M
867 746.4061 1490.7976 1490.7981 -0.28
0
52 0.0056
1
K.EGGSVLTLTYLGGPK.F
Mass: 29118
Score: 155
Queries matched: 3
gi|157165165
gi|223038888
Mass: 29342
Score: 155
Queries matched: 3
enoyl-[acyl-carrier-protein] reductase [NADH] (nadh-dependent enoyl-acp reductase) [Campylobacter rectus RM3267]
gi|255323509
Mass: 29040
Score: 155
Queries matched: 3
enoyl-[acyl-carrier-protein] reductase [Campylobacter showae RM3277]
51.
gi|157164122
Mass: 40793
Score: 152
Queries matched: 5
emPAI: 0.17
DNA polymerase III subunit beta [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
548 606.3656 1210.7166 1210.7173 -0.51
0
25
1
1
K.ELLTVISPLNI.553 609.3120 1216.6094 1216.6088
0.55
0
28
2.3
1
K.ATDHEIGLAYK.L
579 619.3063 1236.5980 1236.5986 -0.46
0
42
0.084
1
K.IVDEGYATANGK.K
900 759.9115 1517.8084 1517.8089 -0.32
0
29
1.5
1
R.DLSAITSHIYISAK.D
1029 840.4355 1678.8564 1678.8566 -0.09
0
27
2.2
1
K.FANEASNEFSLIIPK.K
52.
gi|194038353
Mass: 47192
Score: 151
Queries matched: 6
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
273 493.7795
985.5444
985.5444
0.05
0
29
1.6
1
K.QLVSLDPSK.N
362 529.2803 1056.5460 1056.5465 -0.39
1
29
1.5
6
R.WRDSLQPR.R 363
448 561.3133 1120.6120 1120.6128 -0.69
0
55 0.0031
1
R.DTQSIIFLGK.V 449
538 602.8376 1203.6606 1203.6612 -0.43
1
39
0.14
1
K.LLDKFVQDAR.E
53.
gi|6320157
Mass: 19051
Functional homolog of human
Query Observed
Mr(expt)
10 353.7158
705.4170
577 617.8555 1233.6964
602 625.3301 1248.6456
1242 961.5588 1921.1030
Score: 151
Queries matched: 7
emPAI: 0.38
NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention
Mr(calc)
705.4174 -0.44
0
32
0.71
1
K.YVVVAR.A
1233.6969 -0.35
0
40
0.056
1
K.IVEIPGEVPPGK.Y 574 576
1248.6462 -0.47
0
43
0.073
1
K.EVNLDPNPPVR.G
1921.1037 -0.33
1
37
0.028
1
K.IVEIPGEVPPGKYVVVAR.A 1241
gi|151941952
Mass: 19021
Score: 151
Queries matched: 7
Mass: 19007
Score: 151
Queries matched: 7
gi|190405057
54.
gi|194038351
Mass: 46968
Score: 146
Queries matched: 4
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
30 367.7258
733.4370
733.4374 -0.51
0
25
1.2
1
K.IVDLFK.Q
188 460.7876
919.5606
919.5603
0.36
0
24
0.93
1
R.ITALHLPR.F
538 602.8376 1203.6606 1203.6612 -0.43
1
39
0.14
1
K.LLDKFVQDAR.E
1110 872.9349 1743.8552 1743.8540
0.70
0
57 0.0022
1
K.VFSHQADLSGITDQAR.L
55.
gi|157165398
Mass: 45766
Score: 144
Queries matched: 3
emPAI: 0.15
argininosuccinate synthase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
82 415.2480
828.4814
828.4818 -0.36
0
39
0.31
1
K.GNVTILGR.S
323 509.2823 1016.5500 1016.5502 -0.15
0
56 0.0029
1
R.AIESITLDR.G
737 687.3582 1372.7018 1372.7020 -0.12
0
49
0.014
1
K.MSPAEILTELNR.L
56.
gi|157163909
Mass: 45295
Score: 143
Queries matched: 5
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
140 436.2532
870.4918
870.4923 -0.53
0
38
0.24
1
R.VGSPALTAR.G 139
259 489.2740
976.5334
976.5342 -0.75
0
33
0.7
1
R.SPFITSGIR.V
1104 865.4515 1728.8884 1728.8894 -0.53
0
73 6.2e-005
1
K.IADVLSDINNASLQEK.I 1103
57.
gi|32267367
Mass: 37749
Score: 143
Queries matched: 5
emPAI: 0.18
Query Observed
Mr(expt)
Mr(calc)
42 385.1974
768.3802
768.3806 -0.50
0
49 0.0025
1
K.AADFFAK.R
434 555.3318 1108.6490 1108.6492 -0.12
0
51 0.0047
1
K.KPIIVPEDAK.G
786 711.9197 1421.8248 1421.8242
0.46
1
42
0.012
3
K.KPIIVPEDAKGQK.M 784 785
58.
gi|157165776
Mass: 34805
Score: 141
Queries matched: 4
putative carbon storage regulator-like protein [Campylobacter concisus 13826]
12/9/2009
Query
5
264
596
615
Observed
350.7212
490.2583
623.8630
629.8615
Mr(expt)
699.4278
978.5020
1245.7114
1257.7084
Mr(calc)
699.4279
978.5022
1245.7122
1257.7081
-0.06
0
44
0.11
1
-0.12
0
30
1.6
2
-0.57
0
40
0.073
1
0.29
1
26
2.4
1
Page 13 of 18
Peptide
K.AANLIAK.E
K.LYEENLAK.F
K.VIFVAPQFPTK.A
K.ALIEKFPQNAK.L
59.
gi|157165509
Mass: 37709
DNA-directed RNA polymerase
Query Observed
Mr(expt)
775 708.8881 1415.7616
931 783.4507 1564.8868
945 791.9052 1581.7958
954 799.9026 1597.7906
Score: 141
Queries matched: 4
emPAI: 0.40
subunit alpha [Campylobacter concisus 13826]
Mr(calc)
ppm Miss Score
Expect Rank Peptide
1415.7620 -0.23
0
70 9.7e-005
1
K.LLSSVEDLNLSAR.S
1564.8865
0.23
0
28
0.91
1
R.LLYTSTVGFAPIGVK.I
1581.7960 -0.09
0
43
0.055
1
K.ITTSAYMPTEIEVK.S
1597.7909 -0.16
0
(41)
0.087
1
K.ITTSAYMPTEIEVK.S
60.
gi|157165191
Mass: 45841
adenylosuccinate synthetase
Query Observed
Mr(expt)
353 524.7564 1047.4982
432 554.7850 1107.5554
558 613.3099 1224.6052
569 617.3400 1232.6654
Score: 140
Queries matched: 4
emPAI: 0.07
Mr(calc)
1047.4985 -0.23
0
25
5.4
1
R.YEDLPANAR.A
1107.5560 -0.53
0
37
0.24
1
K.VGYISTSPER.S
1224.6060 -0.63
0
25
2.6
1
R.LDGVDTYALMK.L
1232.6653
0.15
0
52
0.006
1
K.LDVLDGFEVVK.I
61.
gi|291470
Mass: 19537
Query Observed
Mr(expt)
336 516.2613 1030.5080
484 579.7766 1157.5386
847 738.8680 1475.7214
Score: 139
Mr(calc)
1030.5083
1157.5387
1475.7216
Queries matched: 3
emPAI: 0.37
-0.27
0
48
0.024
1
-0.02
0
23
3.2
1
-0.13
0
68 0.00017
1
Peptide
K.YAASSYLTR.T
K.SYSSVSCQVK.H
K.VNDAVTTDGVQTTR.S
gi|291474
Mass: 17423
Score: 139
Queries matched: 3
gi|291478
Mass: 22654
Score: 139
Queries matched: 3
gi|9858133
Mass: 24186
Score: 139
Queries matched: 3
gi|194214037
Mass: 17257
Score: 139
Queries matched: 3
Mass: 15452
Score: 139
Queries matched: 3
gi|194214039
62.
gi|157164944
Mass: 41414
Score:
sigma 54 modulation protein/ribosomal
Query Observed
Mr(expt)
Mr(calc)
344 522.7479 1043.4812 1043.4825
662 645.8423 1289.6700 1289.6689
1088 859.8983 1717.7820 1717.7829
134
Queries matched: 4
emPAI: 0.08
protein S30EA [Campylobacter concisus 13826]
-1.18
0
36
0.13
1
R.NDFQFFAR.V 345
0.87
0
36
0.41
1
R.ELNEMVLFAPK.G
-0.47
0
63 0.00042
1
K.ELSPVVMADETNEER.I
63.
gi|149699777
Mass: 38441
Score: 134
Queries matched: 3
emPAI: 0.09
PREDICTED: similar to Haptoglobin precursor [Equus caballus]
Query Observed
Mr(expt)
Mr(calc)
186 460.7345
919.4544
919.4552 -0.82
0
34
0.64
1
K.GSFPWQAK.L
266 490.7508
979.4870
979.4876 -0.54
0
49
0.015
1
R.VGYVSGWGR.N
985 811.9084 1621.8022 1621.8022
0.05
0
51 0.0098
1
K.YVTLPVADQDTCVK.H
64.
gi|157165110
Mass: 29741
Score: 132
Queries matched: 3
emPAI: 0.24
hydrogenase accessory protein HypB [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
210 472.2947
942.5748
942.5750 -0.13
0
58 0.00085
1
R.AADVLLITK.A
280 495.2973
988.5800
988.5804 -0.40
0
28
0.68
1
K.TTLLEATIK.A
1106 865.9131 1729.8116 1729.8119 -0.13
0
46
0.022
1
K.IGVVEGDLETNQDADR.I
65.
gi|157164063
3-oxoacyl-(acyl
Query Observed
74 409.7601
878 749.3773
1161 909.9608
66.
gi|157164859
Mass: 27476
Score: 124
Queries matched: 3
emPAI: 0.26
Query Observed
Mr(expt)
Mr(calc)
535 601.3248 1200.6350 1200.6350
0.06
0
52
0.01
1
K.GNTELLEAINK.A
623 632.8307 1263.6468 1263.6459
0.72
0
22
10
1
K.NGATVVVADGFSK.G
970 803.3965 1604.7784 1604.7794 -0.62
0
51
0.011
1
K.ADVVFNQVSINEDR.K
67.
gi|15027001
Mass: 42057
Score: 129
Queries matched: 3
emPAI: 0.16
carrier protein) synthase II [Campylobacter concisus 13826]
Mr(expt)
Mr(calc)
817.5056
817.5062 -0.63
0
32
0.18
1
R.FIQLGIK.A
1496.7400 1496.7399
0.11
0
45
0.039
1
K.EITSFDVSEFPVK.I
1817.9070 1817.9047
1.29
0
52 0.0076
1
K.IAAEITDFDPNSILDGK.E
Mass: 36537
Score: 116
Queries matched: 4
emPAI: 0.09
12/9/2009
immunoglobulin gamma 4 heavy
Query
Observed
Mr(expt)
85
417.2313
832.4480
109
426.7503
851.4860
912
771.3623 1540.7100
1357 1071.9970 2141.9794
chain constant region [Equus caballus]
Mr(calc)
832.4477
0.46
0
22
10
1
851.4865 -0.51
0
23
2.2
3
1540.7086
0.96
0
27
1.6
1
2141.9793
0.06
0
43
0.035
1
Page 14 of 18
Peptide
K.DVLMISR.T
K.ALPAPVER.T
K.DFYPTDIDIEWK.S
K.YSTTPAQLDSDGSYFLYSK.L
Mass: 35698
Score: 116
Queries matched: 4
gi|42528293
immunoglobulin gamma 4 heavy chain [Equus caballus]
68.
gi|157164697
Mass: 43758
Score: 116
Queries matched: 4
phosphoglycerate kinase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
105 425.2449
848.4752
848.4756 -0.42
0
28
2.2
1
512 587.8563 1173.6980 1173.6982 -0.13
0
25
0.77
1
902 760.3827 1518.7508 1518.7566 -3.77
0
30
1.5
1
959 801.4226 1600.8306 1600.8308 -0.08
0
34
0.57
1
Peptide
K.FSLQGVAK.R
K.LQALHNLLPR.V
R.EVIFAEDVIGNDAK.T
M.SDILSINDLELNGAK.V
69.
gi|27806941
Mass: 46075
Score: 113
Queries matched: 4
emPAI: 0.15
Query Observed
Mr(expt)
Mr(calc)
6 350.7337
699.4528
699.4531 -0.31
0
33
0.28
1
K.IVELVK.V
315 506.7747 1011.5348 1011.5349 -0.05
0
41
0.05
1
K.GNTHTEILK.G
496 584.8083 1167.6020 1167.6023 -0.21
0
39
0.071
1
K.LSISETYDLK.S 497
70.
gi|158604987
Mass: 46508
Score: 103
Queries matched: 3
emPAI: 0.07
aminoacyl-histidine dipeptidase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
198 467.7713
933.5280
933.5284 -0.34
0
49
0.022
1
K.AVGFNVISK.D
776 709.4115 1416.8084 1416.8089 -0.31
0
31
0.27
1
K.ILALINSFSQGVR.A
817 723.8316 1445.6486 1445.6497 -0.71
0
23
3.8
1
K.IEIVYGDDGYMR.A
71.
gi|154174678
Mass: 36635
Score: 101
Queries matched: 3
Query Observed
Mr(expt)
Mr(calc)
170 453.2639
904.5132
904.5130
0.26
1
41
0.11
1
R.FALKAEAR.D
238 482.7641
963.5136
963.5138 -0.12
1
30
1.2
1
R.RVEATFNK.585 620.8376 1239.6606 1239.6612 -0.42
1
30
0.95
1
R.YQVTDRFALK.A
72.
gi|157165029
Mass: 34028
Score: 98
Queries matched: 2
emPAI: 0.10
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
490 582.3030 1162.5914 1162.5910
0.38
1
20
12
1
K.KYESFLTYI.661 644.8323 1287.6500 1287.6499
0.09
0
78 2.1e-005
1
K.AYFDVVYGLNK.K
73.
gi|157163983
Mass: 42666
Score: 97
Queries matched: 2
emPAI: 0.08
aspartate kinase [Campylobacter concisus 13826]
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
450 562.2919 1122.5692 1122.5670
2.04
0
22
6.6
1
K.FGGTSVGTLER.I
950 799.3930 1596.7714 1596.7705
0.61
0
76 2.8e-005
1
K.ISYDEMLELASAGAK.V
Mass: 42797
Score: 97
Queries matched: 2
gi|158604990
asparate kinase, monofunctional class [Campylobacter concisus 13826]
74.
gi|157164875
Mass: 45924
Score: 94
Queries matched: 2
emPAI: 0.15
glutamate-1-semialdehyde aminotransferase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
642 639.3366 1276.6586 1276.6598 -0.91
0
37
0.21
1
K.VIGGGMNVAAFGGK.A
826 728.8588 1455.7030 1455.7028
0.19
0
57 0.0019
1
R.FVSSGTEATMSAIR.V
75.
gi|157164923
Mass: 39225
Score: 94
Queries matched: 2
emPAI: 0.08
Query Observed
Mr(expt)
Mr(calc)
150 439.7525
877.4904
877.4909 -0.56
0
27
2
1
K.IFGVVDTK.V
721 679.3551 1356.6956 1356.6965 -0.65
0
66 0.00024
1
R.LGYEEFIPYVK.-
76.
gi|157164030
Mass: 45502
Score: 93
Queries matched: 2
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
779 710.8773 1419.7400 1419.7398
0.18
0
25
3.3
1
K.FLEELGLKPNFN.872 747.3959 1492.7772 1492.7773 -0.02
0
67 0.00018
1
K.LSQDELANLLYSK.N
77.
gi|229552
Mass: 66088
albumin
Query Observed
Mr(expt)
Score: 90
Mr(calc)
Queries matched: 3
ppm
emPAI: 0.05
Peptide
12/9/2009
100
491
1000
424.2551
582.3185
820.4720
846.4956
1162.6224
1638.9294
846.4963
1162.6234
1638.9305
-0.80
-0.79
-0.63
1
0
1
31
35
24
0.58
0.34
1.5
2
1
1
Page 15 of 18
R.LSQKFPK.A
K.LVNELTEFAK.T
R.KVPQVSTPTLVEVSR.S
Mass: 69248
Score: 90
Queries matched: 3
gi|1351907
Mass: 69278
Score: 90
Queries matched: 3
gi|30794280
Mass: 69190
Score: 90
Queries matched: 3
gi|74267962
gi|154425704
Mass: 69248
Score: 90
Queries matched: 3
78.
gi|157164579
Mass: 31381
Score: 86
Queries matched: 3
Query Observed
Mr(expt)
Mr(calc)
199 469.2621
936.5096
936.5103 -0.65
0
30
0.56
1
206 471.7842
941.5538
941.5546 -0.78
0
30
0.88
1
473 573.2811 1144.5476 1144.5472
0.36
0
26
2.3
1
Peptide
R.IAFNMITK.M
K.TIGNTPIVK.V
K.VSNDDALNAAR.A
79.
gi|157164144
Mass: 33246
Score: 80
Queries matched: 2
emPAI: 0.10
Query
Observed
Mr(expt)
Mr(calc)
862
744.3959 1486.7772 1486.7780 -0.50
1
30
1.4
1
K.GLFVAGDIREDAPK.Q
1336 1039.4860 2076.9574 2076.9600 -1.22
0
50 0.0089
1
80.
gi|27807349
Mass: 51691
Query Observed
Mr(expt)
439 556.8031 1111.5916
1027 839.9363 1677.8580
81.
gi|123506
Mass: 8353
Score: 79
Queries matched: 2
emPAI: 0.42
Query Observed
Mr(expt)
Mr(calc)
269 492.3032
982.5918
982.5923 -0.49
0
43
0.016
1
R.ALGILNLNR.N
655 644.3456 1286.6766 1286.6765
0.13
0
36
0.36
1
R.NLQLILNSCGR.S
Score: 80
Queries matched: 2
emPAI: 0.06
clade G, member 1 [Bos taurus]
Mr(calc)
1111.5914
0.26
0
30
1
1
R.LYQDFSVLK.K
1677.8573
0.42
0
50
0.011
1
R.SAEAVLGEALTDFSLR.L
gi|5019730
Mass: 12474
Score: 79
Queries matched: 2
gi|76782253
Mass: 12343
Score: 79
Queries matched: 2
82.
gi|504
Bovine
Query
412
1169
Mass: 75134
Score: 77
Lactoferrin [Bos taurus]
Observed
Mr(expt)
Mr(calc)
549.2561 1096.4976 1096.4978
914.4419 1826.8692 1826.8686
Queries matched: 5
emPAI: 0.04
-0.13
0
23
3.6
1
0.33
0
54 0.0049
1
Peptide
K.YYGYTGAFR.C
K.GEADALNLDGGYIYTAGK.C 1167 1168 1170
gi|506
Mass: 78070
Score: 77
Queries matched: 5
Mass: 77873
Score: 77
Queries matched: 5
gi|163285
lactoferrin
gi|408928
Mass: 78117
Score: 77
Queries matched: 5
lactoferrin
Mass: 78025
Score: 77
Queries matched: 5
gi|9650974
gi|22795592
Mass: 38836
Score: 77
Queries matched: 5
gi|30794292
Mass: 78006
Score: 77
Queries matched: 5
gi|34810001
Mass: 37957
Score: 77
Queries matched: 5
gi|46015771
Mass: 36554
Score: 77
Queries matched: 5
gi|83754256
Mass: 37586
Score: 77
Queries matched: 5
gi|146157606
Mass: 78129
Score: 77
Queries matched: 5
gi|146387335
Mass: 37439
Score: 77
Queries matched: 5
gi|157830374
Mass: 76095
Score: 77
Queries matched: 5
gi|193299659
Mass: 78006
Score: 77
Queries matched: 5
gi|255762013
Mass: 76226
Score: 77
Queries matched: 5
At 1.5a Resolution
12/9/2009
Page 16 of 18
83.
gi|28603766
Mass: 45268
Score: 72
Queries matched: 1
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
705 673.3688 1344.7230 1344.7224
0.51
0
72 7.4e-005
1
R.LPIQEAFLGAMR.T
84.
gi|154173772
Mass: 42702
Score: 67
Queries matched: 1
emPAI: 0.08
NifS family cysteine desulfurase [Campylobacter curvus 525.92]
Query Observed
Mr(expt)
Mr(calc)
739 689.3722 1376.7298 1376.7299 -0.07
0
67 0.0002
1
R.TALDQLYAGINAK.D
85.
gi|154175359
Mass: 48388
Score: 67
Queries matched: 3
citrate (Si)-synthase [Campylobacter curvus 525.92]
Query Observed
Mr(expt)
Mr(calc)
468 569.8163 1137.6180 1137.6182 -0.16
0
39
0.13
1
638 635.8615 1269.7084 1269.7081
0.27
0
28
1.7
1
gi|157164090
Mass:
citrate (Si)-synthase
gi|255322867
Mass:
citrate (Si)-synthase
86.
Peptide
K.IPTIAAFSYR.Y 469
R.GLPIIYPNLDR.G
48234
Score: 67
Queries matched: 3
48470
Score: 67
Queries matched: 3
[Campylobacter showae RM3277]
gi|157165526
Mass: 46029
Score:
general glycosylation pathway protein
Query Observed
Mr(expt)
Mr(calc)
241 484.7797
967.5448
967.5450
772 708.4200 1414.8254 1414.8258
67
Queries matched: 2
-0.20
0
33
0.3
1
K.ALANPINQK.S
-0.24
0
34
0.1
1
K.IIILPFGSMTVPK.V
gi|255321958
Mass: 45799
Score: 67
Queries matched: 2
general glycosylation pathway protein [Campylobacter showae RM3277]
87.
gi|157164168
Mass: 34845
Score: 63
Queries matched: 2
emPAI: 0.10
phosphoribosylaminoimidazole synthetase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
502 584.8503 1167.6860 1167.6863 -0.22
0
29
0.41
1
K.LIDVLLEPTR.I
1184 924.4837 1846.9528 1846.9538 -0.49
0
34
0.46
1
K.STQTPNVIGGIGSFSGAVR.L
88.
gi|154173771
Mass: 43606
Score: 62
Queries matched: 2
phosphoglycerate kinase [Campylobacter curvus 525.92]
Query Observed
Mr(expt)
Mr(calc)
331 513.7826 1025.5506 1025.5505
0.11
0
38
0.14
1
512 587.8563 1173.6980 1173.6982 -0.13
0
25
0.77
1
Peptide
R.AHASVEAITK.F
K.LQALHNLLPR.V
gi|223038840
Mass: 43477
Score: 62
Queries matched: 2
phosphoglycerate kinase [Campylobacter rectus RM3267]
89.
gi|154175458
Mass: 18862
ProP protein [Campylobacter
Query Observed
Mr(expt)
293 500.2482
998.4818
562 613.7898 1225.5650
Score: 61
Queries matched: 2
curvus 525.92]
Mr(calc)
998.4821 -0.28
0
34
0.39
1
1225.5649
0.14
0
27
1.9
1
Peptide
K.FHIDNPEK.Q
K.MDTGVGNYELK.F
gi|157165691
Mass: 18808
Score: 61
Queries matched: 2
90.
gi|27806963
Mass: 26002
Score: 59
Queries matched: 2
emPAI: 0.13
Query Observed
Mr(expt)
Mr(calc)
265 490.2841
978.5536
978.5538 -0.19
0
24
4.9
1
K.FALPQYLK.T
731 684.3516 1366.6886 1366.6881
0.42
0
35
0.38
1
K.ALNEINQFYQK.F
91.
gi|157165326
Mass: 18857
Score: 59
Queries matched: 2
Query Observed
Mr(expt)
Mr(calc)
495 584.7726 1167.5306 1167.5309 -0.20
0
25
2.9
1
736 685.8589 1369.7032 1369.7030
0.16
0
34
0.49
1
92.
Peptide
K.AGGHWDPAETK.M
K.TNYGVAFFPNLK.G
gi|157165403
Mass: 22036
Score: 56
Queries matched: 1
emPAI: 0.15
Query Observed
Mr(expt)
Mr(calc)
588 622.8455 1243.6764 1243.6772 -0.60
0
56 0.0028
1
R.AGSGEVGINLLSK.F
12/9/2009
93.
gi|157165668
Mass: 41734
Score: 53
Queries matched: 2
Cys/Met metabolism PLP-dependent enzyme superfamily protein [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
960 801.4303 1600.8460 1600.8461 -0.01
0
25
3.2
1
K.VLEHGADIVVYSATK.Y
1256 971.5200 1941.0254 1941.0248
0.35
0
28
1.3
1
K.GALVIVDNTFLTPYYQK.V
94.
gi|218459
Mass: 41048
Score: 52
Queries matched: 1
Query Observed Mr(expt) Mr(calc)
81 415.2477 828.4808 828.4817 -1.07
0
52
0.013
1
Page 17 of 18
Peptide
R.IGSIVANR.Q
gi|256081
Mass: 41165
Score: 52
Queries matched: 1
HSP150 [Saccharomyces cerevisiae]
gi|404129
Mass: 36354
Score: 52
Queries matched: 1
gi|6322303
Mass: 23227
Score: 52
Queries matched: 1
Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family; Cis3p [Saccharomyces cerevis
gi|6322684
Mass: 34601
Score: 52
Queries matched: 1
O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p
gi|6322685
Mass: 32984
Score: 52
Queries matched: 1
O-glycosylated covalently-bound cell wall protein required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subj
gi|30267752
Mass: 12208
Score: 52
Queries matched: 1
Hsp150p [Saccharomyces cerevisiae]
gi|30267776
Mass: 8323
Score: 52
Queries matched: 1
YJL160Cp [Saccharomyces cerevisiae]
gi|33339732
Mass: 34428
Score: 52
Queries matched: 1
cell wall protein Pir2p [Pichia angusta]
gi|34979819
Mass: 26146
Score: 52
Queries matched: 1
HSP150 [Saccharomyces cerevisiae]
gi|42742254
Mass: 30197
Score: 52
Queries matched: 1
Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is req
gi|50290175
Mass: 34605
Score: 52
Queries matched: 1
hypothetical protein CAGL0I06182g [Candida glabrata CBS138]
gi|50290177
Mass: 35741
Score: 52
Queries matched: 1
hypothetical protein CAGL0I06204g [Candida glabrata CBS138]
gi|50294606
Mass: 34055
Score: 52
Queries matched: 1
hypothetical protein CAGL0M08492g [Candida glabrata CBS138]
gi|116006493
Mass: 41034
Score: 52
Queries matched: 1
O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for c
gi|146413995
Mass: 44534
Score: 52
Queries matched: 1
hypothetical protein PGUG_04923 [Pichia guilliermondii ATCC 6260]
Mass: 32199
Score: 52
Queries matched: 1
gi|156846985
hypothetical protein Kpol_2001p21 [Vanderwaltozyma polyspora DSM 70294]
gi|156846987
Mass: 37012
Score: 52
Queries matched: 1
gi|156846989
Mass: 23015
Score: 52
Queries matched: 1
gi|156847960
Mass: 35811
Score: 52
Queries matched: 1
gi|164710430
Mass: 28983
Score: 52
Queries matched: 1
structural cell wall protein [Saccharomyces cerevisiae]
gi|164710432
Mass: 28997
Score: 52
Queries matched: 1
gi|164710434
Mass: 33271
Score: 52
Queries matched: 1
gi|207343636
Mass: 21137
Score: 52
Queries matched: 1
YKL164Cp-like protein [Saccharomyces cerevisiae AWRI1631]
gi|239977090
Mass: 23014
Score: 52
Queries matched: 1
RecName: Full=Cell wall mannoprotein CIS3; AltName: Full=Covalently-linked cell wall protein 5/11; AltName: Full=Protein with internal repeats 4; Alt
gi|239977282
Mass: 36741
Score: 52
Queries matched: 1
RecName: Full=Cell wall mannoprotein HSP150; AltName: Full=150 kDa heat shock glycoprotein; AltName: Full=Protein with internal repeats 2; AltName: F
gi|239977283
Mass: 30510
Score: 52
Queries matched: 1
gi|239977284
Mass: 38693
Score: 52
Queries matched: 1
gi|239977520
Mass: 34617
Score: 52
Queries matched: 1
RecName: Full=Cell wall mannoprotein PIR1; AltName: Full=Protein with internal repeats 1; AltName: Full=Covalently-linked cell wall protein 6; Flags:
gi|239977521
Mass: 34587
Score: 52
Queries matched: 1
gi|239977522
Mass: 31216
Score: 52
Queries matched: 1
gi|239977523
Mass: 41451
Score: 52
Queries matched: 1
gi|239977524
Mass: 30182
Score: 52
Queries matched: 1
RecName: Full=Cell wall protein PIR5; AltName: Full=Protein with internal repeats 5; Flags: Precursor
gi|239977526
Mass: 30269
Score: 52
Queries matched: 1
RecName: Full=Cell wall protein PIR5; AltName: Full=Protein with internal repeats 5; Flags: Precursor
gi|255716288
Mass: 39663
Score: 52
Queries matched: 1
KLTH0F05016p [Lachancea thermotolerans]
gi|256270853
Mass: 34627
Score: 52
Queries matched: 1
12/9/2009
Page 18 of 18
Pir3p [Saccharomyces cerevisiae JAY291]
gi|256271354
Mass: 30213
Score: 52
Queries matched: 1
YJL160C-like protein [Saccharomyces cerevisiae JAY291]
95.
gi|157164887
Mass: 43293
Score: 52
Queries matched: 1
emPAI: 0.08
NifS family cysteine desulfurase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
698 665.8554 1329.6962 1329.6963 -0.01
0
52 0.0069
1
R.SGTLDVPGIVGMGK.A
96.
gi|112542
Mass: 3333
Score: 50
Queries matched: 2
emPAI: 1.15
164 450.7552 899.4958 899.4964 -0.58
0
50
0.013
1
-.TPLEEAIK.N 165
gi|281063
Mass: 3326
Score: 50
Queries matched: 2
gi|1086940
Mass: 3588
Score: 50
Queries matched: 2
major outer membrane protein, MOMP=porin {N-terminal} [Campylobacter jejuni, Peptide Partial, 31 aa]
97.
gi|154173631
Mass: 19261
Score: 50
Queries matched: 1
emPAI: 0.17
Query Observed
Mr(expt)
Mr(calc)
653 643.8510 1285.6874 1285.6878 -0.25
0
50
0.012
1
K.SNLLDVLNDGVK.-
gi|157165227
Mass: 19284
Score: 50
Queries matched: 1
12/9/2009
Page 1 of 20
User
:
Email
:
Search title
:
MS data file
:
Database
:
Timestamp
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
Protein hits
:
LTQFT Daemon
N_18_7_09_11.RAW relex2
D:\Data\Mark\18_7-09\N_18_7_09_11.RAW
9 Dec 2009 at 04:28:15 GMT
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
1572
gi|157164095 FAD binding domain-containing protein [Campylobacter concisus 13826]
gi|157165518 elongation factor P (EF-P) [Campylobacter concisus 13826]
gi|157165097 chaperonin GroEL [Campylobacter concisus 13826]
gi|28317
gi|84000377 endopin 1b [Bos taurus]
gi|194038349 PREDICTED: similar to SERPINA3-6 [Sus scrofa]
gi|160332365 RecName: Full=Serpin A3-1; AltName: Full=Endopin-1A; AltName: Full=Muscle endopin-1A; Short=mEndopin-1A; Flags: Precu
gi|157165093 trigger factor [Campylobacter concisus 13826]
gi|27806941 serine proteinase inhibitor, clade A, member 1 [Bos taurus]
gi|157164988 flagellin B [Campylobacter concisus 13826]
gi|157165314 thiamine biosynthesis protein ThiC [Campylobacter concisus 13826]
gi|76363596 RecName: Full=Serum albumin; Flags: Precursor
gi|157165243 S-layer-RTX protein [Campylobacter concisus 13826]
gi|42528291 immunoglobulin gamma 7 heavy chain [Equus caballus]
gi|146741296 keratin 1 [Sus scrofa]
gi|181402
gi|28603766 serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 [Bos taurus]
gi|9858135
immunoglobulin G heavy chain [Equus caballus]
gi|194225324 PREDICTED: similar to alpha-1-antitrypsin; serine protease inhibitor [Equus caballus]
gi|154175408 flagellin B [Campylobacter curvus 525.92]
gi|136429
gi|1942778
gi|157165083 transcription termination factor Rho [Campylobacter concisus 13826]
gi|122411
gi|157164344 biotin carboxylase [Campylobacter concisus 13826]
gi|122614
gi|291470
gi|2232299
gi|157165135 ComEC/Rec2 family protein [Campylobacter concisus 13826]
gi|167621418 alpha-1-antitrypsin [Equus caballus]
gi|157165008 transketolase A [Campylobacter concisus 13826]
gi|223041213 extracellular tungstate binding protein [Campylobacter rectus RM3267]
12/9/2009
gi|114051856
gi|157165286
gi|157165527
gi|27807349
gi|157165191
gi|18999435
gi|157165109
gi|172048405
gi|157164634
gi|157164859
gi|157165087
gi|27807209
gi|157165398
gi|157164740
gi|157164819
gi|229552
gi|157164833
gi|255322858
gi|123506
gi|157164553
gi|157164319
gi|154175426
gi|157164353
gi|158604964
gi|157165180
gi|157164241
gi|154173809
gi|42560938
gi|157165609
gi|154148662
gi|157163909
gi|16329620
gi|225632
gi|157347169
gi|16755594
gi|157164015
gi|118475424
gi|6320157
gi|157164517
Page 2 of 20
keratin 7 [Bos taurus]
phosphoenolpyruvate carboxykinase [Campylobacter concisus 13826]
serpin peptidase inhibitor, clade G, member 1 [Bos taurus]
adenylosuccinate synthetase [Campylobacter concisus 13826]
RecName: Full=Formate--tetrahydrofolate ligase; AltName: Full=Formyltetrahydrofolate synthetase; Short=FHS; Short=FTH
D-3-phosphoglycerate dehydrogenase [Campylobacter concisus 13826]
alpha-2-plasmin inhibitor [Bos taurus]
beta ketoacyl-acyl carrier protein synthase II [Campylobacter concisus 13826]
albumin
phosphopyruvate hydratase [Campylobacter concisus 13826]
flagellin B [Campylobacter showae RM3277]
molecular chaperone DnaK [Campylobacter concisus 13826]
succinate dehydrogenase flavoprotein subunit [Campylobacter concisus 13826]
aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (Asp/Glu-ADT subunit B) [Campylobacter concisus 13826]
2-isopropylmalate synthase [Campylobacter concisus 13826]
Na+ ABC transporter, ATP-binding component [Mycoplasma mycoides subsp. mycoides SC str. PG1]
glutamine synthetase, type I [Campylobacter concisus 13826]
phosphomannomutase/phosphoglucomutase (PMM/PGM) [Campylobacter hominis ATCC BAA-381]
methyl-accepting chemotaxis protein [Synechocystis sp. PCC 6803]
casein alphaS1
unnamed protein product [Vitis vinifera]
mucin [Bos taurus]
trimethylamine-n-oxide reductase 1 (tmaoreductase 1; trimethylamine oxidase 1) [Campylobacter concisus 13826]
dihydroxy-acid dehydratase [Campylobacter fetus subsp. fetus 82-40]
192
6
3.13 %
353
6
1.70 %
Format As
Standard scoring
Show sub-sets 0
Require bold red
Show pop-ups
1.
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MudPIT scoring
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gi|157164095
Mass: 55914
Score: 1078
Queries matched: 43
emPAI: 2.94
FAD binding domain-containing protein [Campylobacter concisus 13826]
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
4
350.7337
699.4528
699.4531 -0.31
0
38
0.09
1
K.VVLIEK.M
64
400.2028
798.3910
798.3912 -0.18
0
26
1.4
2
R.YNEFVK.K
68
402.2256
802.4366
802.4371 -0.55
0
30
1.9
1
K.AIAAGICK.K
79
409.2335
816.4524
816.4527 -0.34
0
(23)
7.3
2
K.AIAAGICK.K
199
464.2502
926.4858
926.4861 -0.32
1
37
0.15
1
R.YNEFVKK.G
214
471.2868
940.5590
940.5593 -0.28
1
35
0.18
4
K.IPVDELKK.T
325
506.2317 1010.4488 1010.4491 -0.25
0
30
0.98
1
R.YMNELADR.R
409
540.3138 1078.6130 1078.6135 -0.40
0
44
0.014
1
R.GINHVDLLAK.I 408
442
553.7922 1105.5698 1105.5703 -0.38
0
(41)
0.1
1
K.GVVLAAGGFCR.D
467
560.8001 1119.5856 1119.5859 -0.23
0
61
0.00074
1
K.GVVLAAGGFCR.D
524
584.2820 1166.5494 1166.5502 -0.65
1
28
1.3
2
R.YMNELADRR.T
744
678.8373 1355.6600 1355.6609 -0.61
0
51
0.0065
1
K.FDTLDALAAEYK.I
794
711.3692 1420.7238 1420.7245 -0.47
0
(36)
0.28
1
K.VHHTMGGLNINTK.A 795
796
711.8630 1421.7114 1421.7125 -0.76
0
36
0.33
1
R.ALPHMLPAHYEK.A
818
719.3663 1436.7180 1436.7194 -0.97
0
47
0.02
1
K.VHHTMGGLNINTK.A
828
732.3801 1462.7456 1462.7456
0.02
0
53
0.0078
1
K.GIDISVPPFYAER.G 829
867
750.3202 1498.6258 1498.6246
0.84
0
61
0.00022
1
K.FDGNLMSDDVENK.G 866
1012
825.9208 1649.8270 1649.8274 -0.22
0
63
0.0007
1
K.YIDQITHAGGHSVPR.S 1008 1011
1020
555.2892 1662.8458 1662.8465 -0.44
0
43
0.066
1
K.GVDEDFGKPVVATTTK.G 1019
1142
896.4775 1790.9404 1790.9415 -0.56
1
60
0.0012
1
K.KGVDEDFGKPVVATTTK.G 1143
1268
653.2859 1956.8359 1956.8371 -0.63
1
41
0.026
1
K.FDGNLMSDDVENKGGDTK.Y
1350 1053.5180 2105.0214 2105.0212
0.13
0
46
0.029
1
R.TGGNSVINGGIFAVPNSDMQK.K
1362 1061.5150 2121.0154 2121.0161 -0.30
0
(33)
0.58
1
1431 1118.5810 2235.1474 2235.1457
0.79
0
54
0.0037
1
R.SLQTEVGSGAGIVLPMTATFEK.L 1430
1437 1126.5780 2251.1414 2251.1406
0.37
0
(48)
0.018
1
R.SLQTEVGSGAGIVLPMTATFEK.L
12/9/2009
1460
1556
1560
1561
1563
1159.5860
984.1852
1019.8340
1019.8340
1025.1660
2317.1574
2949.5338
3056.4802
3056.4802
3072.4762
2317.1550
2949.5369
3056.4808
3056.4808
3072.4757
1.06
-1.05
-0.20
-0.20
0.16
0
1
0
0
0
79 1.6e-005
55
0.0017
61
0.00077
(27)
2
(46)
0.026
Page 3 of 20
1
1
1
1
1
R.ITPETDSTNQPGSTAGALLAAFR.A 1457 1458 1459
R.SLQTEVGSGAGIVLPMTATFEKLEGASIK.K
K.AQVMNSQTAMPIPNLYAAGEITGGVHGASR.L
K.AQVMNSQTAMPIPNLYAAGEITGGVHGASR.L 1562
2.
gi|157165518
Mass: 53621
Score: 992
Queries matched: 28
emPAI: 1.60
elongation factor P (EF-P) [Campylobacter concisus 13826]
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
22
360.1896
718.3646
718.3650 -0.46
0
36
0.11
1
K.SGEWIK.N
178
453.2762
904.5378
904.5382 -0.36
1
33
0.33
1
K.FIEAAVKK.F
213
470.3152
938.6158
938.6164 -0.61
1
38
0.014
1
R.LIDKILPK.G
217
471.7899
941.5652
941.5658 -0.60
0
52
0.0084
1
K.GLINIVTGR.G 216
313
502.7756 1003.5366 1003.5372 -0.55
0
24
5.4
1
K.NIMIDLTGK.V
429
546.8002 1091.5858 1091.5862 -0.36
1
36
0.29
1
K.FKDEAELIK.M
502
576.3058 1150.5970 1150.5982 -1.03
0
35
0.29
1
K.LAFTGSTEIGR.D
626
623.3162 1244.6178 1244.6183 -0.36
0
29
1.4
1
K.VESMDNGKPIR.E
642
627.8169 1253.6192 1253.6193 -0.04
0
33
0.43
1
K.YGLFINGEWR.D
658
634.3902 1266.7658 1266.7659 -0.08
1
50
0.0022
1
R.RIIPATLELGGK.S
672
638.3415 1274.6684 1274.6693 -0.64
0
44
0.057
1
K.IILEHYTQMK.N
690
646.3392 1290.6638 1290.6642 -0.26
0
(32)
0.87
1
K.IILEHYTQMK.N
821
721.3514 1440.6882 1440.6885 -0.17
0
63
0.00053
1
K.SANIFFSDANLDK.A
884
759.3580 1516.7014 1516.7005
0.61
0
90 7.6e-007
1
K.IADATEEDVNDAVR.A
945
792.9532 1583.8918 1583.8923 -0.29
0
66
0.00013
1
R.VAQEEIFGPVGVVIK.F 946 947
994
816.9514 1631.8882 1631.8882
0.01
1
42
0.047
1
K.QAEQILNYVEIGKK.E 993
1162
607.9742 1820.9008 1820.9017 -0.49
1
25
3.9
1
K.DGATLDAKNPANGEHLAK.I
1211
938.9771 1875.9396 1875.9401 -0.22
0
48
0.019
1
K.GAFVEPTLLVDVTNDMR.V
1224
946.9739 1891.9332 1891.9350 -0.92
0
(38)
0.2
1
K.GAFVEPTLLVDVTNDMR.V
1305 1014.9830 2027.9514 2027.9510
0.23
0
84 3.8e-006
1
R.YFAGVIMGEEGSANVLDEK.Q
1328 1022.9790 2043.9434 2043.9459 -1.20
0
(83) 4.1e-006
1
1354 1056.5300 2111.0454 2111.0476 -1.03
0
55
0.004
1
R.VWINTYNQIPAGSPFGGYK.N
1389 1073.0130 2144.0114 2144.0096
0.86
0
(75) 3.4e-005
1
K.MVNDSEYGLGGGIFTQDITK.A
1402 1081.0090 2160.0034 2160.0045 -0.49
0
108 1.3e-008
1
K.MVNDSEYGLGGGIFTQDITK.A
3.
gi|194038347
Mass: 46611
Score: 837
Queries matched: 42
emPAI: 0.98
PREDICTED: alpha-1-antichymotrypsin 3 [Sus scrofa]
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
24
360.1913
718.3680
718.3683 -0.40
0
28
2.8
1
R.MAAVEAK.L 23
59
396.7159
791.4172
791.4177 -0.62
0
33
0.63
1
K.LLDEFR.Q
96
417.2222
832.4298
832.4299 -0.06
0
29
2.5
1
K.VPMMSLR.G 95
116
425.2195
848.4244
848.4248 -0.44
0
(22)
6.5
5
K.VPMMSLR.G
117
425.2195
848.4244
848.4248 -0.44
0
(29)
1.5
1
K.VPMMSLR.G
244
480.7791
959.5436
959.5440 -0.36
0
52
0.012
1
R.IDALYLPR.F 245
275
491.7531
981.4916
981.4920 -0.33
0
34
0.65
1
R.GLETPYFR.D
284
493.7794
985.5442
985.5444 -0.16
0
50
0.012
1
K.QLVSLDPSK.N 283
302
499.3110
996.6074
996.6080 -0.54
1
30
0.34
1
K.LLPETLRR.W
382
529.2803 1056.5460 1056.5465 -0.39
1
34
0.45
1
R.WRDSLQPR.R 378 379 381 383
462
558.8296 1115.6446 1115.6451 -0.40
1
49
0.0086
1
R.RIDALYLPR.F 463
488
572.2874 1142.5602 1142.5608 -0.48
0
32
0.58
1
R.FSVSGDYQLK.D
651
631.8062 1261.5978 1261.5979 -0.05
0
60
0.0012
1
K.FASDVFSTNFK.D
664
635.8658 1269.7170 1269.7180 -0.75
0
54
0.0021
1
R.GPTLTELLEGLK.F 665
812
717.3727 1432.7308 1432.7310 -0.12
1
38
0.19
1
K.LLDEFRQDAQAK.F 811
875
754.3938 1506.7730 1506.7719
0.79
0
31
1.2
1
K.DPGAAVVFINDYVK.N
968
800.9146 1599.8146 1599.8144
0.13
1
66
0.00027
1
R.FSVSGDYQLKDTLK.Q
1043
845.4074 1688.8002 1688.8006 -0.21
0
80 1.1e-005
1
K.VFTHEADLSGVTGDNK.L 1041 1042
1285
657.9846 1970.9320 1970.9374 -2.77
0
25
3.1
1
K.TPFDPEHTIESTFHVSK.N
1410 1087.5580 2173.1014 2173.1015 -0.04
1
69
0.00013
1
K.VFTHEADLSGVTGDNKLGVSK.V 1406 1407 1408 1409 1411 1412
1464
773.7269 2318.1589 2318.1583
0.24
0
45
0.042
1
R.TPVDPHAIVPSNTDFAFSLYK.Q 1462 1463
4.
gi|157164629
Mass: 43586
Score: 807
Queries matched: 40
emPAI: 3.31
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
98
417.2397
832.4648
832.4655 -0.73
0
23
4.5
2
R.TVGSGVVSK.I
149
434.2557
866.4968
866.4974 -0.61
0
33
0.32
1
R.EHILLSR.Q 148
366
524.7385 1047.4624 1047.4621
0.31
0
56
0.0014
1
K.AGQDGEWSAK.I 365
386
530.3112 1058.6078 1058.6084 -0.53
1
28
1
1
R.GTVVTGRIEK.G
563
599.8212 1197.6278 1197.6281 -0.23
0
40
0.071
1
K.FEGEVYILTK.E
698
652.3827 1302.7508 1302.7507
0.11
0
69 4.8e-005
1
K.TTLTAAISAVLSR.K
861
498.6105 1492.8097 1492.8112 -1.04
0
32
0.51
1
R.QVGVPYIVVFMNK.A 862
877
755.4103 1508.8060 1508.8061 -0.06
0
(20)
7
1
R.QVGVPYIVVFMNK.A
1045
845.4080 1688.8014 1688.8005
0.54
1
29
1.5
1
1055
848.9775 1695.9404 1695.9407 -0.13
0
54
0.0021
1
1062
851.9069 1701.7992 1701.7992
0.03
0
(52)
0.0054
1
K.EMDQGEAGDNVGVLLR.G 1061
1081
859.9043 1717.7940 1717.7941 -0.04
0
82 5.6e-006
1
1185
924.4781 1846.9416 1846.9425 -0.44
1
70
0.00012
1
R.GITIATSHIEYETEKR.H 1184
1266
975.4884 1948.9622 1948.9638 -0.80
0
(41)
0.1
1
1280
983.4857 1964.9568 1964.9587 -0.96
0
(64)
0.0005
1
1281
983.4860 1964.9574 1964.9587 -0.65
0
(46)
0.03
1
K.IMELMDAVDSYIPTPVR.A 1282
1288
991.4839 1980.9532 1980.9536 -0.20
0
82 7.6e-006
1
1352 1054.0410 2106.0674 2106.0667
0.36
1
59
0.0016
1
1366
708.3611 2122.0615 2122.0616 -0.07
1
(43)
0.057
1
R.ATDKDLLMPIEDVFSISGR.G 1367 1368
1470 1190.0890 2378.1634 2378.1642 -0.30
0
49
0.016
1
R.ELLNEYNFPGDDTPIVSGSALK.A 1471
12/9/2009
1511
1515
1517
1519
1530
1546
1260.6010
846.0682
1268.6000
851.4000
903.4775
940.8434
2519.1874
2535.1828
2535.1854
2551.1782
2707.4107
2819.5084
2519.1884
2535.1833
2535.1833
2551.1782
2707.4103
2819.5103
-0.37
-0.21
0.85
-0.02
0.15
-0.69
0
0
0
0
0
1
(42)
(39)
(34)
53
22
27
0.067
0.14
0.43
0.0044
4.8
0.84
Page 4 of 20
1
1
1
1
1
1
K.VGDTIEIVGIKPTQTTTVTGVEMFRK.E 1547
5.
gi|194676990
Mass: 45494
Score:
Query Observed
Mr(expt)
Mr(calc)
15 357.6981
713.3816
713.3820
57 396.2421
790.4696
790.4701
100 417.7048
833.3950
833.3953
119 425.7024
849.3902
849.3902
157 439.7526
877.4906
877.4909
203 465.7661
929.5176
929.5182
286 493.7975
985.5804
985.5808
297 497.8060
993.5974
993.5971
345 513.8005 1025.5864 1025.5869
405 540.2956 1078.5766 1078.5771
459 557.8448 1113.6750 1113.6757
588 608.3039 1214.5932 1214.5931
1114 884.4126 1766.8106 1766.8112
1141 896.4250 1790.8354 1790.8363
1229 632.6423 1894.9051 1894.9061
1485 811.6989 2432.0749 2432.0781
1500 831.3976 2491.1710 2491.1754
1541 915.1403 2742.3991 2742.3977
752
Queries matched: 41
emPAI: 1.01
taurus]
Expect Rank Peptide
ppm Miss Score
-0.52
0
33
0.33
1
R.NSLQPR.H 14 16
-0.58
1
32
1
1
K.LLDKFR.K
-0.28
0
35
0.31
1
K.MQDLEAK.L
0.06
0
(23)
5.4
7
K.MQDLEAK.L
-0.28
0
37
0.22
1
K.LINEYVK.N
-0.58
0
39
0.2
1
K.LTPETLTR.W
-0.34
0
48
0.019
1
R.DILSQLGIK.K 285 287
0.34
0
27
0.51
1
K.LAVSQVIHK.A
-0.45
1
33
0.33
1
K.QLALKDPNK.N 344
-0.41
0
57
0.0019
1
-0.62
1
30
0.45
1
R.DILSQLGIKK.I 460
0.09
0
69
0.00013
1
R.FSISSDYQLR.D 587 589 590
-0.29
0
100 8.5e-008
1
K.IFTSDADFSGITNDHK.L 1112 1113
-0.46
0
55
0.003
1
K.DAEAFYASEVLSTNFK.D
-0.55
1
49
0.015
1
K.KIFTSDADFSGITNDHK.L 1227 1230 1232 1233
-1.34
0
34
0.24
1
-1.79
1
46
0.025
1
K.DAEAFYASEVLSTNFKDSEAAVK.L 1501 1502 1503
0.50
1
29
1
1
K.IFTSDADFSGITNDHKLAVSQVIHK.A 1538 1540
6.
gi|157165097
Mass: 57700
Score: 735
Queries matched: 21
emPAI: 0.84
chaperonin GroEL [Campylobacter concisus 13826]
Query
Observed
Mr(expt)
Mr(calc)
207
467.7531
933.4916
933.4920 -0.35
0
27
5
1
K.SFGAPNITK.D
583
606.8327 1211.6508 1211.6510 -0.11
0
67 0.00018
1
R.NVTAGANPIEVK.R
591
609.8071 1217.5996 1217.6000 -0.31
1
45
0.034
1
K.DRVDDALSATR.A
691
647.3214 1292.6282 1292.6282
0.05
1
39
0.13
1
K.VGAATETEMKEK.K
707
657.8196 1313.6246 1313.6252 -0.38
1
53
0.0052
1
M.AKEIFYSDDAR.N
731
672.8718 1343.7290 1343.7296 -0.44
1
45
0.036
1
K.VGKDGVITVEEAK.S
755
684.8837 1367.7528 1367.7521
0.55
1
38
0.088
1
R.NVTAGANPIEVKR.G
896
764.8877 1527.7608 1527.7609 -0.05
0
53
0.0047
1
R.GYLSPYFITNPEK.M 895
963
799.8805 1597.7464 1597.7471 -0.42
1
71
9e-005
1
K.AQIAETTSDYDKEK.L
1183
923.9728 1845.9310 1845.9319 -0.49
0
69 0.00013
1
K.EIAQIATISANSDESIGK.L
1187
926.0509 1850.0872 1850.0877 -0.23
1
34
0.034
1
K.ITNLKDLLPVLEQVQK.S
1205
934.9383 1867.8620 1867.8622 -0.08
0
38
0.12
1
K.SIQDELSVVEGMQFDR.G
1239
952.4932 1902.9718 1902.9720 -0.10
1
44
0.044
1
K.EVELKDTIENMGASLVR.E 1237 1238
1251
640.6629 1918.9669 1918.9670 -0.05
1
(30)
1.1
1
K.EVELKDTIENMGASLVR.E
1348
697.6837 2090.0293 2090.0280
0.61
0
39
0.15
1
R.QIAENAGFDAGVVANAVETSK.D
1374 1066.5350 2131.0554 2131.0546
0.41
0
26
2.9
1
K.TNDQAGDGTTTATVLAHAIFK.E
1567 1087.5530 3259.6372 3259.6420 -1.47
1
46
0.02
1
R.TLESATINDLGQASSVVIDKDNTTIVNGAGEK.S 1568
7.
gi|28317
Mass: 59492
Score: 727
Query
Observed
Mr(expt)
Mr(calc)
74
404.2031
806.3916
806.3923
355
516.3027 1030.5908 1030.5910
392
532.8085 1063.6024 1063.6026
423
545.7687 1089.5228 1089.5237
440
553.7665 1105.5184 1105.5186
522
583.2957 1164.5768 1164.5775
604
617.8427 1233.6708 1233.6717
650
631.8025 1261.5904 1261.5899
750
683.3235 1364.6324 1364.6320
769
695.8440 1389.6734 1389.6736
816
717.8888 1433.7630 1433.7626
858
747.3709 1492.7272 1492.7270
1069
854.3896 1706.7646 1706.7649
1346 1041.9840 2081.9534 2081.9575
1423
738.0363 2211.0871 2211.0906
1552
963.4681 2887.3825 2887.3804
Queries matched: 17
emPAI: 0.46
ppm Miss Score
Expect Rank
-0.76
0
31
0.77
1
-0.17
0
56
0.0013
1
-0.11
1
55
0.0025
1
-0.77
0
(40)
0.1
1
-0.14
0
45
0.04
1
-0.53
0
48
0.022
1
-0.68
1
35
0.37
1
0.47
0
67
0.00017
1
0.31
0
30
0.91
1
-0.08
0
93 5.6e-007
1
0.29
1
36
0.2
1
0.18
1
44
0.046
1
-0.15
0
110 6.2e-009
1
-1.96
0
33
0.36
1
-1.61
1
22
6.4
1
0.72
0
21
6.8
1
Peptide
R.LAADDFR.L
R.VLDELTLTK.A
R.LASYLDKVR.A
K.VTMQNLNDR.L
K.VTMQNLNDR.L
R.LENEIQTYR.S
R.LKYENEVALR.Q 605
R.SLLEGEGSSGGGGR.G
R.SQYEQLAEQNR.K
K.QSLEASLAETEGR.Y
K.IRLENEIQTYR.S
R.SQYEQLAEQNRK.D
R.AETECQNTEYQQLLDIK.I
K.SKELTTEIDNNIEQISSYK.S
R.NVSTGDVNVEMNAAPGVDLTQLLNNMR.S
Mass: 58792
Score: 727
Queries matched: 17
gi|21961605
gi|114667509
Mass: 62761
Score: 727
Queries matched: 17
gi|114667511
Mass: 58244
Score: 727
Queries matched: 17
gi|114667513
Mass: 56692
Score: 727
Queries matched: 17
gi|119581085
Mass: 63308
Score: 727
Queries matched: 17
12/9/2009
Page 5 of 20
gi|195972866
Mass: 58766
Score: 727
Queries matched: 17
gi|251757513
Mass: 58818
Score: 727
Queries matched: 17
gi|114667507
Mass: 100282
Score: 724
Queries matched: 17
8.
gi|11935049
Mass: 66027
Query Observed
Mr(expt)
94 416.7477
831.4808
153 437.7530
873.4914
261 487.2691
972.5236
305 500.2259
998.4372
332 508.2234 1014.4322
359 517.2614 1032.5082
394 533.2641 1064.5136
486 571.2634 1140.5122
541 590.3036 1178.5926
654 633.3217 1264.6288
697 651.8611 1301.7076
726 670.8380 1339.6614
762 692.3485 1382.6824
771 697.3698 1392.7250
844 738.3957 1474.7768
1000 819.9338 1637.8530
1077 858.9292 1715.8438
Score: 701
Mr(calc)
831.4814
873.4920
972.5240
998.4379
1014.4328
1032.5087
1064.5138
1140.5121
1178.5931
1264.6299
1301.7078
1339.6619
1382.6830
1392.7249
1474.7780
1637.8525
1715.8438
10.
gi|62126072
Mass: 46687
Query Observed
Mr(expt)
15 357.6981
713.3816
57 396.2421
790.4696
100 417.7048
833.3950
113 424.2298
846.4450
119 425.7024
849.3902
136 432.2271
862.4396
137 432.2274
862.4402
157 439.7526
877.4906
158 440.2248
878.4350
198 463.2423
924.4700
203 465.7661
929.5176
297 497.8060
993.5974
345 513.8005 1025.5864
403 537.8116 1073.6086
405 540.2956 1078.5766
425 364.2083 1089.6031
426 364.2084 1089.6034
443 369.5401 1105.5985
482 568.3214 1134.6282
588 608.3039 1214.5932
1114 884.4126 1766.8106
1229 632.6423 1894.9051
1541 915.1403 2742.3991
gi|121531628
Mass: 46282
Query
Observed
Mr(expt)
15
357.6981
713.3816
49
389.7206
777.4266
100
417.7048
833.3950
119
425.7024
849.3902
226
476.2607
950.5068
243
480.7626
959.5106
327
506.7947 1011.5748
345
513.8005 1025.5864
468
561.3135 1120.6124
529
584.8453 1167.6760
553
596.8320 1191.6494
592
407.2274 1218.6604
664
635.8658 1269.7170
737
673.8691 1345.7236
1321 1021.5010 2040.9874
1330 1029.4990 2056.9834
1338
691.6689 2071.9849
1341 1037.4970 2072.9794
1380
714.6881 2141.0425
Score: 696
Mr(calc)
713.3820
790.4701
833.3953
846.4456
849.3902
862.4405
862.4405
877.4909
878.4354
924.4705
929.5182
993.5971
1025.5869
1073.6089
1078.5771
1089.6039
1089.6039
1105.5988
1134.6285
1214.5931
1766.8112
1894.9061
2742.3977
Peptide
K.SISISVAR.G
R.SLVNLGGSK.S
K.IEISELNR.V
K.DVDGAYMTK.V
K.DVDGAYMTK.V
R.TLLEGEESR.M
K.AQYEDIAQK.S
R.DYQELMNTK.L
K.YEELQITAGR.H
R.TNAENEFVTIK.K
R.SLDLDSIIAEVK.A
K.SKAEAESLYQSK.Y
K.SLNNQFASFIDK.V
R.TNAENEFVTIKK.D 772
R.FLEQQNQVLQTK.W
K.SLNNQFASFIDKVR.F
K.QISNLQQSISDAEQR.G
Queries matched: 18
Queries matched: 18
Queries matched: 40
emPAI: 0.85
Expect Rank
ppm Miss Score
-0.52
0
33
0.33
1
-0.58
1
32
1
1
-0.28
0
35
0.31
1
-0.60
0
38
0.27
1
0.06
0
(23)
5.4
7
-0.96
0
(20)
10
1
-0.26
0
(20)
11
1
-0.28
0
37
0.22
1
-0.39
0
(29)
1.8
1
-0.49
0
26
1.7
1
-0.58
0
39
0.2
1
0.34
0
27
0.51
1
-0.45
1
33
0.33
1
-0.26
1
(36)
0.22
1
-0.41
0
57
0.0019
1
-0.71
1
(30)
0.8
1
-0.44
1
39
0.091
1
-0.27
1
(27)
1.8
1
-0.19
0
51
0.0059
1
0.09
0
69
0.00013
1
-0.29
0
100 8.5e-008
1
-0.55
1
49
0.015
1
0.50
1
29
1
1
Score: 677
Mr(calc)
713.3820
777.4272
833.3953
849.3902
950.5073
959.5110
1011.5753
1025.5869
1120.6128
1167.6764
1191.6499
1218.6608
1269.7180
1345.7241
2040.9900
2056.9850
2071.9851
2072.9799
2141.0429
emPAI: 0.40
ppm Miss Score
Expect Rank
-0.66
0
27
1.8
1
-0.60
0
39
0.28
1
-0.33
0
43
0.1
1
-0.65
0
(23)
2.3
1
-0.56
0
32
0.15
1
-0.46
0
33
0.44
1
-0.15
0
26
3.9
1
0.13
0
34
0.3
1
-0.40
0
39
0.18
1
-0.84
0
29
1.4
1
-0.14
0
88 1.8e-006
1
-0.35
1
49
0.013
1
-0.41
0
56
0.0028
1
0.13
1
44
0.042
1
-0.77
0
51
0.0091
1
0.31
1
27
1.6
1
0.02
0
81 7.7e-006
1
gi|119395750
Mass: 65999
Score: 701
gi|160961491
Mass: 65450
Score: 701
9.
Queries matched: 18
Queries matched: 34
Peptide
R.NSLQPR.H 14 16
K.LLDKFR.K
K.MQDLEAK.L
K.VPMMTLR.L 114
K.MQDLEAK.L
K.VPMMTLR.L
K.VPMMTLR.L
K.LINEYVK.N
K.VPMMTLR.L
R.LETPYFR.D
K.LTPETLTR.W
K.LAVSQVIHK.A
K.QLALKDPNK.N 344
R.VKVPMMTLR.L
R.VKVPMMTLR.L
R.VKVPMMTLR.L
R.VKVPMMTLR.L
K.ETQSIIFLGK.V
R.FSISSDYQLR.D 587 589 590
K.IFTSDADFSGITNDHK.L 1112 1113
K.KIFTSDADFSGITNDHK.L 1227 1230 1232 1233
K.IFTSDADFSGITNDHKLAVSQVIHK.A 1538 1540
emPAI: 1.45
-0.52
0
33
0.33
1
-0.75
0
26
2.2
1
-0.28
0
35
0.31
1
0.06
0
(23)
5.4
7
-0.45
0
54
0.0046
1
-0.35
0
33
0.61
1
-0.43
0
31
0.62
1
-0.45
1
33
0.33
1
-0.33
0
64 0.00037
1
-0.29
1
29
0.59
1
-0.39
1
38
0.15
1
-0.36
1
31
1.1
1
-0.75
0
54
0.0021
1
-0.36
1
60
0.0013
1
-1.27
0
(48)
0.016
1
-0.73
0
(34)
0.39
1
-0.11
0
40
0.1
1
-0.21
0
49
0.012
1
-0.20
1
37
0.24
1
Peptide
R.NSLQPR.R 14 16
K.IEELFK.D
K.MQDLEAK.L
K.MQDLEAK.L
K.SLINDYVK.N
K.LTPEMLTR.W
R.IHELYLPK.F 328
K.QLALKDPNK.N 344
K.DTQSIIFLGK.V
R.RIHELYLPK.F
K.TQGKIEELFK.D
K.LLDKFIEDAR.V
R.GPTLTEILEGLK.F 665
K.IEELFKDLSPR.T 735 736 738
K.TVEVPMMTLDLETPYFR.D
R.VDGHTLASSNTDFAFSLYK.Q 1339 1340
K.TVEVPMMTLDLETPYFR.D 1342
R.VLYSSEAFPTNFRDPEAAK.S 1379 1381 1382
12/9/2009
1426
11.
743.7023
2228.0851
gi|84000377
Mass: 46297
Query
Observed
Mr(expt)
15
357.6981
713.3816
49
389.7206
777.4266
100
417.7048
833.3950
119
425.7024
849.3902
226
476.2607
950.5068
243
480.7626
959.5106
327
506.7947 1011.5748
343
513.3087 1024.6028
468
561.3135 1120.6124
529
584.8453 1167.6760
553
596.8320 1191.6494
592
407.2274 1218.6604
664
635.8658 1269.7170
737
673.8691 1345.7236
1321 1021.5010 2040.9874
1330 1029.4990 2056.9834
1338
691.6689 2071.9849
1341 1037.4970 2072.9794
1380
714.6881 2141.0425
1426
743.7023 2228.0851
2228.0862
-0.50
Score: 666
Mr(calc)
713.3820
777.4272
833.3953
849.3902
950.5073
959.5110
1011.5753
1024.6029
1120.6128
1167.6764
1191.6499
1218.6608
1269.7180
1345.7241
2040.9900
2056.9850
2071.9851
2072.9799
2141.0429
2228.0862
1
28
1.9
Queries matched: 33
Page 6 of 20
1
R.RVDGHTLASSNTDFAFSLYK.Q
emPAI: 1.45
-0.52
0
33
0.33
1
-0.75
0
26
2.2
1
-0.28
0
35
0.31
1
0.06
0
(23)
5.4
7
-0.45
0
54
0.0046
1
-0.35
0
33
0.61
1
-0.43
0
31
0.62
1
-0.04
1
22
5.4
1
-0.33
0
64 0.00037
1
-0.29
1
29
0.59
1
-0.39
1
38
0.15
1
-0.36
1
31
1.1
1
-0.75
0
54
0.0021
1
-0.36
1
60
0.0013
1
-1.27
0
(48)
0.016
1
-0.73
0
(34)
0.39
1
-0.11
0
40
0.1
1
-0.21
0
49
0.012
1
-0.20
1
37
0.24
1
-0.50
1
28
1.9
1
Peptide
R.NSLQPR.R 14 16
K.IEELFK.D
K.MQDLEAK.L
K.MQDLEAK.L
K.SLINDYVK.N
K.LTPEMLTR.W
R.IHELYLPK.F 328
K.QLALKNPNK.N
K.DTQSIIFLGK.V
R.RIHELYLPK.F
K.TQGKIEELFK.D
K.LLDKFIEDAR.V
R.VLYSSEAFPTNFRDPEAAK.S 1379 1381 1382
12.
gi|194038349
Mass: 38243
Score: 647
Queries matched: 31
emPAI: 1.29
PREDICTED: similar to SERPINA3-6 [Sus scrofa]
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
59
396.7159
791.4172
791.4177 -0.62
0
33
0.63
1
K.LLDEFR.Q
244
480.7791
959.5436
959.5440 -0.36
0
52
0.012
1
R.IDALYLPR.F 245
284
493.7794
985.5442
985.5444 -0.16
0
50
0.012
1
K.QLVSLDPSK.N 283
462
558.8296 1115.6446 1115.6451 -0.40
1
49
0.0086
1
R.RIDALYLPR.F 463
651
631.8062 1261.5978 1261.5979 -0.05
0
60
0.0012
1
K.FASDVFSTNFK.D
664
635.8658 1269.7170 1269.7180 -0.75
0
54
0.0021
1
812
717.3727 1432.7308 1432.7310 -0.12
1
38
0.19
1
K.LLDEFRQDAQAK.F 811
974
801.3883 1600.7620 1600.7621 -0.01
0
95 2.8e-007
1
R.FSVSGDYQLEDTLK.Q 972 973 975 976
1043
845.4074 1688.8002 1688.8006 -0.21
0
80 1.1e-005
1
1410 1087.5580 2173.1014 2173.1015 -0.04
1
69
0.00013
1
K.VFTHEADLSGVTGDNKLGVSK.V 1406 1407 1408 1409 1411 1412
1440
757.0613 2268.1621 2268.1638 -0.75
1
22
7
1
R.FSVSGDYQLEDTLKQLGIQK.V
1464
773.7269 2318.1589 2318.1583
0.24
0
45
0.042
1
R.TPVDPHAIVPSNTDFAFSLYK.Q 1462 1463
13.
gi|160332365
Mass: 46208
Score: 607
Queries matched: 23
emPAI: 1.29
Query
Observed
Mr(expt)
Mr(calc)
15
357.6981
713.3816
713.3820 -0.52
0
33
0.33
1
R.NSLQPR.R 14 16
49
389.7206
777.4266
777.4272 -0.75
0
26
2.2
1
K.IEELFK.Y
203
465.7661
929.5176
929.5182 -0.58
0
39
0.2
1
K.LTPETLTR.W
226
476.2607
950.5068
950.5073 -0.45
0
54
0.0046
1
R.SLINDYVK.N
327
506.7947 1011.5748 1011.5753 -0.43
0
31
0.62
1
R.IHELYLPK.F 328
343
513.3087 1024.6028 1024.6029 -0.04
1
22
5.4
1
K.QLALKNPNK.N
468
561.3135 1120.6124 1120.6128 -0.33
0
64 0.00037
1
K.DTQSIIFLGK.V
529
584.8453 1167.6760 1167.6764 -0.29
1
29
0.59
1
R.RIHELYLPK.F
553
596.8320 1191.6494 1191.6499 -0.39
1
38
0.15
1
K.TQGKIEELFK.Y
648
630.8561 1259.6976 1259.6973
0.30
0
53
0.0042
1
R.GSTLTEILEGLK.F
1093
867.9551 1733.8956 1733.8948
0.50
0
74
4e-005
1
K.SNYELNDILSQLGIR.K
1200
932.0023 1861.9900 1861.9897
0.17
1
24
3.4
1
K.SNYELNDILSQLGIRK.I
1321 1021.5010 2040.9874 2040.9900 -1.27
0
(48)
0.016
1
1330 1029.4990 2056.9834 2056.9850 -0.73
0
(34)
0.39
1
1338
691.6689 2071.9849 2071.9851 -0.11
0
40
0.1
1
1341 1037.4970 2072.9794 2072.9799 -0.21
0
49
0.012
1
1426
743.7023 2228.0851 2228.0862 -0.50
1
28
1.9
1
gi|226373737
Mass: 46208
Score: 607
14.
gi|38683423
Mass: 46972
Query Observed
Mr(expt)
15 357.6981
713.3816
57 396.2421
790.4696
100 417.7048
833.3950
119 425.7024
849.3902
157 439.7526
877.4906
203 465.7661
929.5176
286 493.7975
985.5804
312 502.3058 1002.5970
345 513.8005 1025.5864
459 557.8448 1113.6750
482 568.3214 1134.6282
Score: 603
Mr(calc)
713.3820
790.4701
833.3953
849.3902
877.4909
929.5182
985.5808
1002.5974
1025.5869
1113.6757
1134.6285
Queries matched: 23
Queries matched: 26
emPAI: 0.40
-0.52
0
33
0.33
1
-0.58
1
32
1
1
-0.28
0
35
0.31
1
0.06
0
(23)
5.4
7
-0.28
0
37
0.22
1
-0.58
0
39
0.2
1
-0.34
0
48
0.019
1
-0.39
0
38
0.064
1
-0.45
1
33
0.33
1
-0.62
1
30
0.45
1
-0.19
0
51 0.0059
1
Peptide
R.NSLQPR.L 14 16
K.LLDKFR.K
K.MQDLEAK.L
K.MQDLEAK.L
K.LINEYVK.N
K.LTPETLTR.W
K.DILSQLGIK.K 285 287
K.LAVSHVIHK.A
K.QLALKDPNK.N 344
K.DILSQLGIKK.I 460
K.ETQSIIFLGK.V
12/9/2009
577
1116
1141
1485
1500
605.3165
884.9033
896.4250
811.6989
831.3976
1208.6184
1767.7920
1790.8354
2432.0749
2491.1710
1208.6190
1767.7952
1790.8363
2432.0781
2491.1754
-0.42
-1.77
-0.46
-1.34
-1.79
0
0
0
0
1
40
53
55
34
46
0.14
0.0031
0.003
0.24
0.025
1
1
1
1
1
Page 7 of 20
R.FSISSHYQLK.D
K.IFTSDADFSGITDDHK.L
K.DAEAFYASEVLSTNFKDSEAAVK.L 1501 1502 1503
15.
gi|119914036
Mass: 34009
Score: 584
Queries matched: 29
emPAI: 2.36
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
15
357.6981
713.3816
713.3820 -0.52
0
33
0.33
1
R.NSLQPR.- 14 16
49
389.7206
777.4266
777.4272 -0.75
0
26
2.2
1
K.IEELFK.D
100
417.7048
833.3950
833.3953 -0.28
0
35
0.31
1
K.MQDLEAK.L
119
425.7024
849.3902
849.3902
0.06
0
(23)
5.4
7
K.MQDLEAK.L
226
476.2607
950.5068
950.5073 -0.45
0
54
0.0046
1
K.SLINDYVK.K
243
480.7626
959.5106
959.5110 -0.35
0
33
0.61
1
K.LTPEMLTR.W
343
513.3087 1024.6028 1024.6029 -0.04
1
22
5.4
1
K.QLALKNPNK.N
553
596.8320 1191.6494 1191.6499 -0.39
1
38
0.15
1
K.TQGKIEELFK.D
592
407.2274 1218.6604 1218.6608 -0.36
1
31
1.1
1
K.LLDKFIEDAR.V
664
635.8658 1269.7170 1269.7180 -0.75
0
54
0.0021
1
737
673.8691 1345.7236 1345.7241 -0.36
1
60
0.0013
1
1321 1021.5010 2040.9874 2040.9900 -1.27
0
(48)
0.016
1
1326 1021.9890 2041.9634 2041.9633
0.09
0
79 1.3e-005
1
R.VLYSSEAFPTNFGDPEAAK.S 1324 1325 1327
1330 1029.4990 2056.9834 2056.9850 -0.73
0
(34)
0.39
1
1338
691.6689 2071.9849 2071.9851 -0.11
0
40
0.1
1
1341 1037.4970 2072.9794 2072.9799 -0.21
0
49
0.012
1
1426
743.7023 2228.0851 2228.0862 -0.50
1
28
1.9
1
16.
gi|157165093
Mass: 50503
Score: 568
Queries matched: 18
emPAI: 0.88
trigger factor [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
138 432.7265
863.4384
863.4389 -0.47
0
31
0.84
1
K.FAEAAVEK.F
160 440.7497
879.4848
879.4854 -0.68
1
44
0.036
1
K.FKFDVPK.N
193 460.2262
918.4378
918.4381 -0.32
0
22
6.9
3
K.AHLEMYR.N
257 486.2707
970.5268
970.5270 -0.12
0
31
0.56
1
R.NQGMLPAIK.M
288 494.2681
986.5216
986.5219 -0.24
0
(24)
3.8
1
R.NQGMLPAIK.M
301 499.2709
996.5272
996.5280 -0.77
0
27
1.7
1
K.LFSELFNK.E
415 542.3240 1082.6334 1082.6335 -0.06
0
47
0.0071
1
K.LINEELKPK.F 414
547 595.8426 1189.6706 1189.6707 -0.01
0
67
0.00017
1
R.LTFIIDELAR.V
694 650.3431 1298.6716 1298.6718 -0.10
0
46
0.025
1
K.NDDLIGEPIVSK.F
775 701.8446 1401.6746 1401.6736
0.77
0
54
0.004
1
K.ELTNDAEQDVLR.D
805 714.3907 1426.7668 1426.7667
0.08
1
57
0.0017
1
K.KNDDLIGEPIVSK.F
891 761.3720 1520.7294 1520.7293
0.09
0
67
0.00023
1
K.NIVEQEIDMQFR.N 890
900 769.3685 1536.7224 1536.7242 -1.16
0
(61)
0.0012
1
K.NIVEQEIDMQFR.N 901
1249 958.9734 1915.9322 1915.9316
0.35
0
76 3.8e-005
1
K.VSDQEVVQAIYFEAYR.S 1248
17.
gi|126165236
Mass: 46368
Score: 556
Queries matched: 23
emPAI: 0.86
Query
Observed
Mr(expt)
Mr(calc)
15
357.6981
713.3816
713.3820 -0.52
0
33
0.33
1
R.NSLQPR.R 14 16
49
389.7206
777.4266
777.4272 -0.75
0
26
2.2
1
K.IEELFK.Y
100
417.7048
833.3950
833.3953 -0.28
0
35
0.31
1
K.MQDLEAK.L
119
425.7024
849.3902
849.3902
0.06
0
(23)
5.4
7
K.MQDLEAK.L
203
465.7661
929.5176
929.5182 -0.58
0
39
0.2
1
K.LTPETLTR.W
226
476.2607
950.5068
950.5073 -0.45
0
54
0.0046
1
R.SLINDYVK.N
327
506.7947 1011.5748 1011.5753 -0.43
0
31
0.62
1
R.IHELYLPK.F 328
343
513.3087 1024.6028 1024.6029 -0.04
1
22
5.4
1
K.QLALKNPNK.N
468
561.3135 1120.6124 1120.6128 -0.33
0
64 0.00037
1
K.DTQSIIFLGK.V
529
584.8453 1167.6760 1167.6764 -0.29
1
29
0.59
1
R.RIHELYLPK.F
553
596.8320 1191.6494 1191.6499 -0.39
1
38
0.15
1
K.TQGKIEELFK.Y
592
407.2274 1218.6604 1218.6608 -0.36
1
31
1.1
1
K.LLDKFIEDAR.V
664
635.8658 1269.7170 1269.7180 -0.75
0
54
0.0021
1
704
656.8174 1311.6202 1311.6208 -0.39
0
48
0.015
1
K.HTEQAEFHVSK.N 705
1321 1021.5010 2040.9874 2040.9900 -1.27
0
(48)
0.016
1
1330 1029.4990 2056.9834 2056.9850 -0.73
0
(34)
0.39
1
1341 1037.4970 2072.9794 2072.9799 -0.21
0
49
0.012
1
18.
gi|194038351
Mass: 46968
Score: 553
Queries matched: 17
emPAI: 0.72
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
35 367.7258
733.4370
733.4374 -0.51
0
32
0.26
1
K.IVDLFK.Q
86 412.7403
823.4660
823.4664 -0.48
0
34
0.22
1
K.VSQVVHR.A
196 460.7873
919.5600
919.5603 -0.29
0
42
0.016
1
R.ITALHLPR.F
572 602.8375 1203.6604 1203.6612 -0.59
1
58
0.0019
1
K.LLDKFVQDAR.E 573
648 630.8561 1259.6976 1259.6973
0.30
0
53
0.0042
1
R.GSTLTELLEGLK.F
908 771.4214 1540.8282 1540.8283 -0.02
0
(23)
3.7
1
R.MAAVEAQLLPETLR.R
928 779.4192 1556.8238 1556.8232
0.42
0
68
0.00013
1
R.MAAVEAQLLPETLR.R
944 792.8809 1583.7472 1583.7467
0.32
0
86 2.3e-006
1
K.DSDAAVEFINNYVK.N
1058 566.6505 1696.9297 1696.9294
0.17
1
24
2.3
1
R.MAAVEAQLLPETLRR.W
1073 571.9819 1712.9239 1712.9243 -0.25
1
(22)
3.9
1
R.MAAVEAQLLPETLRR.W
1097 872.9340 1743.8534 1743.8540 -0.33
0
69
0.00015
1
K.VFSHQADLSGITDQAR.L 1096
1208 624.9899 1871.9479 1871.9490 -0.59
1
38
0.19
1
K.KVFSHQADLSGITDQAR.L 1209
12/9/2009
1478
1484
806.0811
811.4123
2415.2215
2431.2151
2415.2216
2431.2165
-0.06
-0.60
0
0
(23)
50
5.2
0.013
1
1
Page 8 of 20
R.ALSQPSNLLQLNVGNAMFVDER.L
R.ALSQPSNLLQLNVGNAMFVDER.L
19.
gi|114667176
Mass: 107386
Score:
Query Observed
Mr(expt)
Mr(calc)
74 404.2031
806.3916
806.3923
185 457.2079
912.4012
912.4011
348 515.2998 1028.5850 1028.5866
387 530.7851 1059.5556 1059.5560
392 532.8085 1063.6024 1063.6026
423 545.7687 1089.5228 1089.5237
440 553.7665 1105.5184 1105.5186
507 579.2988 1156.5830 1156.5836
602 616.8021 1231.5896 1231.5906
609 618.2679 1234.5212 1234.5215
1140 896.3671 1790.7196 1790.7205
1507 837.3823 2509.1251 2509.1245
517
Queries matched: 13
emPAI: 0.16
ppm Miss Score
Expect Rank Peptide
-0.76
0
31
0.77
1
R.LAADDFR.T
0.14
0
24
2.3
1
R.MTLDDFR.I
-1.50
0
34
0.47
1
R.VLDELTLAR.A
-0.36
0
35
0.43
1
K.TLLDIDNTR.M
-0.11
1
55
0.0025
1
R.LASYLDKVR.A
-0.77
0
(40)
0.1
1
K.VTMQNLNDR.L
-0.14
0
45
0.04
1
K.VTMQNLNDR.L
-0.52
0
40
0.087
1
R.QGVDADINGLR.Q
-0.74
0
86 2.5e-006
1
-0.17
0
55
0.00094
1
R.FSSSSGYGGGSSR.V
-0.47
0
56
0.00033
1
0.24
0
56
0.0015
1
K.EIETYHNLLEGGQEDFESSGAGK.I 1506
20.
gi|194038353
Mass: 47192
Score: 488
Queries matched: 21
emPAI: 0.40
Query Observed
Mr(expt)
Mr(calc)
110 421.7888
841.5630
841.5637 -0.73
1
50
0.0031
1
K.IVELIKK.L
210 467.7767
933.5388
933.5396 -0.79
0
40
0.077
1
R.IDALHLPR.F 209
284 493.7794
985.5442
985.5444 -0.16
0
50
0.012
1
K.QLVSLDPSK.N 283
382 529.2803 1056.5460 1056.5465 -0.39
1
34
0.45
1
R.WRDSLQPR.R 378 379 381 383
468 561.3135 1120.6124 1120.6128 -0.33
0
64 0.00037
1
R.DTQSIIFLGK.V
572 602.8375 1203.6604 1203.6612 -0.59
1
58
0.0019
1
K.LLDKFVQDAR.E 573
785 706.8826 1411.7506 1411.7500
0.46
0
33
0.43
1
R.FDSPFLFATVLR.D
808 715.4072 1428.7998 1428.8017 -1.28
0
46
0.024
1
R.TVAVLVNYIYFK.A 809
927 779.4100 1556.8054 1556.8054
0.02
0
41
0.085
1
R.MAAVEANLLPEMLR.R
935 787.4078 1572.8010 1572.8003
0.45
0
(30)
0.95
1
R.MAAVEANLLPEMLR.R
1072 571.9761 1712.9065 1712.9065 -0.03
1
29
1
1
R.MAAVEANLLPEMLRR.W
1145 599.6517 1795.9333 1795.9356 -1.28
1
22
6.6
1
R.FSISSDYKLEEILPR.L
1432 746.6866 2237.0380 2237.0389 -0.41
0
21
6.5
1
R.ELYHSEAFPTNFQDLEAAR.R
21.
gi|157164029
Mass: 49918
Score: 484
Queries matched: 9
emPAI: 0.56
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
171 451.2662
900.5178
900.5181 -0.31
0
23
3.1
1
K.TLVWINR.G
333 508.3020 1014.5894 1014.5896 -0.13
0
48
0.013
1
R.GGIMQGLVIK.390 531.3135 1060.6124 1060.6128 -0.35
0
36
0.11
1
K.LSVVNISTTK.T
555 597.2811 1192.5476 1192.5473
0.33
0
48
0.013
1
K.FNEANQDVTR.V
723 667.8875 1333.7604 1333.7605 -0.06
0
82 2.4e-006
1
R.GYLVGINSAILSK.S
840 736.4038 1470.7930 1470.7930
0.03
0
51
0.007
1
K.ISPDTQGVLVTDVK.S
1010 825.9206 1649.8266 1649.8260
0.37
0
81 9.7e-006
1
K.IEANGLNAITFADSSK.L
1017 831.9170 1661.8194 1661.8195 -0.05
0
41
0.095
1
1287 986.4885 1970.9624 1970.9619
0.29
0
74 4.2e-005
1
22.
gi|27806941
Mass: 46075
Score: 446
Queries matched: 12
emPAI: 0.32
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
2 350.7336
699.4526
699.4531 -0.59
0
33
0.3
1
K.IVELVK.V
70 402.7265
803.4384
803.4389 -0.53
0
41
0.16
1
R.ADLSGITK.E
159 440.7242
879.4338
879.4338
0.08
0
32
0.98
1
K.INDYVEK.G
318 504.7714 1007.5282 1007.5287 -0.48
1
24
6.7
1
K.KINDYVEK.G
326 506.7745 1011.5344 1011.5349 -0.44
0
26
1.2
1
K.GNTHTEILK.G
527 584.8082 1167.6018 1167.6023 -0.38
0
53
0.0031
1
K.LSISETYDLK.S
540 589.8187 1177.6228 1177.6231 -0.18
0
67
0.00017
1
K.LVDTFLEDVK.N
565 600.8226 1199.6306 1199.6298
0.68
0
25
3
1
K.YASSANLHLPK.L
712 659.8173 1317.6200 1317.6201 -0.05
0
25
3.3
1
R.DFHVDEQTTVK.V
953 797.4092 1592.8038 1592.8046 -0.49
0
77 2.7e-005
1
K.SVLGDVGITEVFSDR.A 954
1119 884.9943 1767.9740 1767.9730
0.59
1
43
0.02
1
K.LQQLEDKLNNELLAK.F
23.
gi|157164988
Mass: 52381
Score: 383
Queries matched: 8
emPAI: 0.44
flagellin B [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
76 405.2112
808.4078
808.4079 -0.10
0
30
1.6
1
R.ATFDVTR.T
788 708.8175 1415.6204 1415.6205 -0.02
0
87
1e-006
1
R.DVDFASESANFSK.H
1154 902.4575 1802.9004 1802.9010 -0.31
0
118 1.9e-009
1
R.IQTAADDASGLSIADSLR.S
1413 730.0101 2187.0085 2187.0080
0.22
1
41
0.085
1
K.SAESQIRDVDFASESANFSK.H
1481 808.4137 2422.2193 2422.2200 -0.31
0
45
0.032
1
R.INTNVNALNTHANAVSNNVDLSK.S 1482
1483 809.0939 2424.2599 2424.2608 -0.40
0
31
0.71
1
R.SQASALGQAIANGNDAIGIIQVADK.A
1526 890.8057 2669.3953 2669.3984 -1.19
0
32
0.46
1
R.SDLGSVQNQLQATVNNITVTQVNVK.S
24.
gi|157164930
Mass: 58243
Score: 377
Queries matched: 11
emPAI: 0.39
Query Observed
Mr(expt)
Mr(calc)
38 377.2103
752.4060
752.4068 -1.06
0
25
2.7
1
K.TAFSISK.V
187 458.2482
914.4818
914.4821 -0.31
0
46
0.034
1
K.IVGDENLR.L
12/9/2009
342
636
789
950
1015
1059
1075
1235
512.2710
625.8530
709.3879
795.4487
830.4273
850.4108
858.4081
948.9704
1022.5274
1249.6914
1416.7612
1588.8828
1658.8400
1698.8070
1714.8016
1895.9262
1022.5284
1249.6918
1416.7612
1588.8824
1658.8416
1698.8076
1714.8025
1895.9265
-0.94
-0.27
0.00
0.27
-0.95
-0.31
-0.49
-0.13
0
1
0
1
0
0
0
1
49
0.013
39
0.069
45
0.041
38
0.078
46
0.028
55 0.0034
(52) 0.0057
33
0.52
1
1
1
1
1
1
1
1
Page 9 of 20
K.SVEILGDYK.V
K.SVEILGDYKVK.I
K.LNAPAISNYEVVK.S
R.IALNYAVNKDEIVK.K
K.LFHGYASVANNPIEK.S 1016
R.ALMFNFNDPLFQDK.N
R.ALMFNFNDPLFQDK.N
K.DIATDKFNDAPIGTGAYK.L
25.
gi|157165314
Mass: 50806
Score: 376
Queries matched: 14
emPAI: 0.29
thiamine biosynthesis protein ThiC [Campylobacter concisus 13826]
Query
Observed
Mr(expt)
Mr(calc)
5
351.2005
700.3864
700.3868 -0.45
0
32
2.1
4
K.IANDLR.L
6
351.2132
700.4118
700.4119 -0.09
0
36
0.63
1
K.IADALAK.A
357
516.7605 1031.5064 1031.5070 -0.51
0
56 0.0031
1
K.AVGDICLER.G
360
517.2618 1032.5090 1032.5096 -0.53
0
25
3.4
2
K.NMLNPSMVK.369
525.2595 1048.5044 1048.5045 -0.07
0
(24)
5.1
1
K.NMLNPSMVK.438
552.3062 1102.5978 1102.5982 -0.32
1
30
1.1
1
K.SDIEKITAAR.E
461
558.7894 1115.5642 1115.5645 -0.20
0
25
3.8
1
K.LTANPEACLK.S
499
575.2589 1148.5032 1148.5033 -0.03
0
54
0.002
1
R.ATENFHMSGR.T
500
575.3289 1148.6432 1148.6441 -0.76
1
22
3.8
2
K.LKEITGVDFK.K
687
644.3529 1286.6912 1286.6911
0.15
1
24
4.7
1
R.TLKEYPYFVK.A 686
1357 1057.5610 2113.1074 2113.1096 -1.00
0
34
0.32
1
K.SVLGSVGIVTAFNPYIGYEK.S
1479
807.4073 2419.2001 2419.2020 -0.78
1
38
0.16
1
K.KADDFIAATQDTADFVHVSGALK.T 1480
26.
gi|126165264
SERPINA3-8 [Bos
Query Observed
35 367.7258
57 396.2421
100 417.7048
119 425.7024
218 472.7399
226 476.2607
343 513.3087
468 561.3135
1124 887.4465
Mass: 46888
taurus]
Mr(expt)
733.4370
790.4696
833.3950
849.3902
943.4652
950.5068
1024.6028
1120.6124
1772.8784
Score: 374
Mr(calc)
733.4374
790.4701
833.3953
849.3902
943.4651
950.5073
1024.6029
1120.6128
1772.8792
Queries matched: 9
emPAI: 0.23
-0.51
0
32
0.26
1
-0.58
1
32
1
1
-0.28
0
35
0.31
1
0.06
0
(23)
5.4
7
0.17
0
35
0.2
1
-0.45
0
54
0.0046
1
-0.04
1
22
5.4
1
-0.33
0
64 0.00037
1
-0.44
0
100
1e-007
1
gi|151554250
Mass: 46935
Score: 374
Peptide
K.IVDLFK.D
K.LLDKFR.E
K.MQDLEAK.L
K.MQDLEAK.L
R.FSIATDYK.L
K.SLINDYVK.N
K.QLALKNPNK.N
K.DTQSIIFLGK.V
Queries matched: 9
27.
gi|47523844
Mass: 47164
Score: 361
Queries matched: 10
emPAI: 0.31
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
37 375.7054
749.3962
749.3959
0.40
0
22
5.5
1
K.FLEDVK.N
334 508.3109 1014.6072 1014.6073 -0.08
0
40
0.073
1
K.SLLGNLGITK.V
452 555.8055 1109.5964 1109.5968 -0.35
0
35
0.18
1
K.LTISGTYDLK.S
598 613.8299 1225.6452 1225.6455 -0.20
0
28
1.4
1
R.YPSSANLHLPK.L
619 622.3215 1242.6284 1242.6278
0.52
0
26
2.4
1
K.LHQLEDMLTK.E
960 799.4217 1596.8288 1596.8300 -0.72
0
86 2.4e-006
1
R.IAPNLADFAFSLYR.Q 961 962
1279 982.4778 1962.9410 1962.9422 -0.59
0
94
4e-007
1
1454 764.7250 2291.1532 2291.1533 -0.04
1
29
1.4
1
K.VFSDEADLSGVTEEQPLKLSK.A
28.
gi|76363596
Mass: 68494
Score: 360
Queries matched: 9
emPAI: 0.21
RecName: Full=Serum albumin; Flags: Precursor
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
112 422.7480
843.4814
843.4814
0.08
1
35
1.3
1
K.LDALKER.I
115 424.2554
846.4962
846.4963 -0.09
1
40
0.062
1
R.LSQKFPK.A
144 433.7164
865.4182
865.4181
0.13
0
40
0.15
1
K.ADFAEVSK.I
232 477.7425
953.4704
953.4706 -0.11
0
25
2
1
K.TYEATLEK.C
240 479.7816
957.5486
957.5495 -0.86
0
58
0.0025
1
K.QSALAELVK.H
639 627.3480 1252.6814 1252.6816 -0.11
0
54
0.0031
1
K.TVLGNFSAFVAK.C
817 718.8277 1435.6408 1435.6402
0.47
0
39
0.1
1
K.YICEHQDSISGK.L
957 798.4590 1594.9034 1594.9043 -0.50
1
70 3.2e-005
1
K.KAPQVSTPTLVEIGR.T 956
gi|126723507
Mass: 68554
Score: 360
preproalbumin [Equus caballus]
29.
Queries matched: 9
gi|157165243
Mass: 55988
Score: 351
Queries matched: 9
emPAI: 0.26
S-layer-RTX protein [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
272 489.7658
977.5170
977.5182 -1.15
0
25
4.3
1
K.LLNEFSQK.T
308 501.2972 1000.5798 1000.5804 -0.60
0
62 0.00046
1
K.LLGEIVETK.G
389 531.2768 1060.5390 1060.5400 -0.93
0
23
5.2
1
K.DLTQELTNK.V
466 560.7743 1119.5340 1119.5342 -0.17
0
51
0.01
1
K.ASNDGSMLVAR.S
481 568.2905 1134.5664 1134.5669 -0.42
0
26
4.5
1
R.YTTIANSHTK.L
594 612.8583 1223.7020 1223.7026 -0.48
0
29
0.66
1
K.AQVFLIHPATK.N
914 773.3708 1544.7270 1544.7260
0.71
0
53
0.0043
1
K.AHDGANDFTYPLPK.D 913
997 819.4001 1636.7856 1636.7845
0.68
0
81
1e-005
1
K.IPQDEYFVTANQGR.L
12/9/2009
Page 10 of 20
30.
gi|42528291
Mass: 35699
Query
Observed
Mr(expt)
93
416.7297
831.4448
97
417.2308
832.4470
194
460.2672
918.5198
416
542.8090 1083.6034
674
639.8609 1277.7072
907
771.3615 1540.7084
1111
882.4227 1762.8308
1132
889.4305 1776.8464
1217
627.6666 1879.9780
1384 1071.9960 2141.9774
31.
gi|115495209
zymogen granule
Query Observed
33 367.2338
73 403.7113
165 443.7280
173 451.7255
512 580.7902
537 587.7975
652 632.7742
653 633.2860
680 642.7741
729 671.8052
743 678.8124
1472 804.6765
1545 939.7750
32.
gi|157163977
Mass: 36762
Score: 337
Queries matched: 10
emPAI: 0.41
Query Observed
Mr(expt)
Mr(calc)
45 385.1974
768.3802
768.3806 -0.50
0
23
0.93
1
K.AADFFAK.R
104 421.2261
840.4376
840.4382 -0.60
0
25
1.6
1
K.FYDVIGK.E
131 429.7501
857.4856
857.4858 -0.19
0
25
5.1
4
K.VLEEQIK.A
219 473.7511
945.4876
945.4879 -0.30
1
35
0.42
1
K.RAEELSGGK.I
475 564.3142 1126.6138 1126.6135
0.31
0
59
0.0013
1
K.FAHVVAASTPK.G
489 572.7958 1143.5770 1143.5772 -0.10
0
36
0.24
2
K.VQDGAVGEELK.G
613 619.8041 1237.5936 1237.5938 -0.16
0
41
0.077
1
K.QALSEATAYER.E
739 675.3476 1348.6806 1348.6809 -0.19
0
66 0.00037
1
K.LGNVQMAAPSFSK.F
751 683.3449 1364.6752 1364.6758 -0.42
0
(60) 0.0013
1
K.LGNVQMAAPSFSK.F
825 727.4000 1452.7854 1452.7864 -0.66
1
26
1.7
1
K.FYDVIGKELIEK.T
33.
gi|146741296
Mass: 65160
Query Observed
Mr(expt)
153 437.7530
873.4914
261 487.2691
972.5236
359 517.2614 1032.5082
446 554.2748 1106.5350
486 571.2634 1140.5122
762 692.3485 1382.6824
844 738.3957 1474.7768
1000 819.9338 1637.8530
34.
Score: 350
Queries matched: 14
emPAI: 0.43
chain [Equus caballus]
Mr(calc)
ppm Miss Score
Expect Rank Peptide
831.4450 -0.21
0
24
9.3
3
K.LTVETNR.W
832.4477 -0.74
0
31
1.5
1
K.DVLMISR.T
918.5208 -1.08
0
38
0.17
1
K.VSVTCLVK.D
1083.6037 -0.19
0
40
0.088
1
K.TISKPTGQPR.E
1277.7092 -1.50
0
26
1.2
1
K.VNNQALPAPVQK.T
1540.7086 -0.08
0
35
0.24
1
K.DFYPTDIDIEWK.S
1762.8308
0.01
0
(59)
0.0012
1
K.SQTYICNVAHPASSTK.V
1776.8465 -0.01
0
74 3.9e-005
1
1879.9792 -0.65
1
25
2.8
1
R.EPQVYVLAPHRDELSK.N 1216 1219
2141.9793 -0.88
0
55
0.0022
1
K.YSTTPAQLDSDGSYFLYSK.L 1385 1386
Mass: 59194
Score:
Mr(expt)
Mr(calc)
732.4530
732.4534
805.4080
805.4083
885.4414
885.4419
901.4364
901.4368
1159.5658 1159.5656
1173.5804 1173.5812
1263.5338 1263.5336
1264.5574 1264.5580
1283.5336 1283.5340
1341.5958 1341.5958
1355.6102 1355.6115
2411.0077 2411.0084
2816.3032 2816.3035
348
Queries matched: 14
emPAI: 0.24
2 [Bos taurus]
-0.48
0
42
0.024
1
R.FNLVLK.N
-0.29
0
22
5
1
R.FVGDGGVR.M
-0.47
0
41
0.035
1
R.FSVQMFK.F
-0.37
0
(38) 0.088
1
R.FSVQMFK.F
0.26
0
(27)
2.4
1
R.LEGTPQCSLR.Y
-0.63
0
45
0.038
1
R.LEGTPQCSLR.Y
0.22
0
21
2.5
2
R.TCRPEEECR.L
-0.46
0
25
2.4
1
R.MPEDCVPTFR.C
-0.25
0
32
0.18
1
R.YCTDPATAEDK.C
0.01
0
(22)
3.8
1
R.LVSGTWGCFCR.Q
-0.91
0
32
0.41
1
R.LVSGTWGCFCR.Q
-0.31
1
37
0.042
1
R.STENTEDIQGCDSDKHGWYR.F 1473
-0.12
0
52 0.0051
1
R.QSEEENWISVTNPTQAGACGNILER.N
Score: 330
Mr(calc)
873.4920
972.5240
1032.5087
1106.5356
1140.5121
1382.6830
1474.7780
1637.8525
gi|181402
Mass: 65825
Score: 310
Query Observed
Mr(expt)
Mr(calc)
261 487.2691
972.5236
972.5240
446 554.2748 1106.5350 1106.5356
641 627.8071 1253.5996 1253.6001
720 665.3666 1328.7186 1328.7187
827 730.9035 1459.7924 1459.7922
844 738.3957 1474.7768 1474.7780
Queries matched: 8
emPAI: 0.10
-0.60
0
39
0.28
1
-0.33
0
43
0.1
1
-0.46
0
33
0.44
1
-0.50
0
45
0.044
1
0.13
0
34
0.3
1
-0.41
0
56 0.0028
1
-0.77
0
51 0.0091
1
0.31
1
27
1.6
1
Queries matched: 6
Peptide
R.SLVNLGGSK.S
K.IEISELNR.V
R.TLLEGEESR.M
K.AQYEEIAQR.S
R.DYQELMNTK.L
K.SLNNQFASFIDK.V
R.FLEQQNQVLQTK.W
K.SLNNQFASFIDKVR.F
emPAI: 0.22
-0.33
0
43
0.1
1
-0.50
0
45
0.044
1
-0.32
0
60 0.00085
1
-0.05
0
53
0.0051
1
0.16
0
57
0.0017
1
-0.77
0
51
0.0091
1
Peptide
K.IEISELNR.V
K.AQYEEIAQR.S
R.GFSSGSAVVSGGSR.R
R.NLDLDSIIAEVK.A
K.VDLLNQEIEFLK.V
R.FLEQQNQVLQTK.W
gi|47132620
Mass: 65393
Score: 310
Queries matched: 6
gi|148727309
Mass: 65944
Score: 310
Queries matched: 6
35.
gi|28603766
Mass: 45268
Score: 307
Queries matched: 9
emPAI: 0.33
Query
Observed
Mr(expt)
Mr(calc)
85
411.7211
821.4276
821.4283 -0.79
0
35
0.5
1
K.INDYVAK.Q
90
415.2154
828.4162
828.4164 -0.14
0
22
4.3
1
R.VSEMVHK.A
155
438.2651
874.5156
874.5164 -0.83
0
21
6.8
1
R.LELYLPK.F
12/9/2009
732
1243
1370
1521
673.3681
954.4595
1065.0070
855.4247
1344.7216
1906.9044
2127.9994
2563.2523
1344.7224
1906.9061
2128.0001
2563.2514
-0.53
-0.88
-0.29
0.34
0
0
0
0
66
68
63
33
0.00032
0.00018
0.00042
0.53
1
1
1
1
Page 11 of 20
R.LPIQEAFLGAMR.T 733
K.STHEQDFYVTPETVVR.V 1244
R.TLYLADTFPTNFEDPEGAK.K
K.AQILEGLGIGPGEGSEEELHSASQR.L
36.
gi|15027001
Mass: 36537
Query
Observed
Mr(expt)
93
416.7297
831.4448
97
417.2308
832.4470
416
542.8090 1083.6034
480
567.8129 1133.6112
907
771.3615 1540.7084
1111
882.4227 1762.8308
1132
889.4305 1776.8464
1384 1071.9960 2141.9774
Score: 299
Queries matched: 10
emPAI: 0.42
Mr(calc)
ppm Miss Score
Expect Rank Peptide
831.4450 -0.21
0
24
9.3
3
K.LTVETNR.W
832.4477 -0.74
0
31
1.5
1
K.DVLMISR.T
1083.6037 -0.19
0
40
0.088
1
R.TISKPTGQPR.E
1133.6115 -0.18
0
39
0.19
1
R.TNVSVTCLVK.D
1540.7086 -0.08
0
35
0.24
1
K.DFYPTDIDIEWK.S
1762.8308
0.01
0
(59)
0.0012
1
1776.8465 -0.01
0
74 3.9e-005
1
2141.9793 -0.88
0
55
0.0022
1
K.YSTTPAQLDSDGSYFLYSK.L 1385 1386
37.
gi|157165374
Mass: 33156
Score: 299
Queries matched: 5
emPAI: 0.33
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
520
582.7856 1163.5566 1163.5571 -0.37
1
22
7.9
1
K.FDGAKDENLR.S
763
692.8333 1383.6520 1383.6518
0.18
0
84 4.1e-006
1
K.DSGLTSDLSSFAGK.K
1027
838.3997 1674.7848 1674.7850 -0.07
0
53
0.0049
1
1083
860.0084 1718.0022 1718.0018
0.24
0
25
0.39
1
1295 1005.9920 2009.9694 2009.9694
0.01
0
115 3.9e-009
1
38.
gi|157164709
Mass: 47282
Score: 293
Queries matched: 8
emPAI: 0.22
Query Observed
Mr(expt)
Mr(calc)
281 492.7953
983.5760
983.5764 -0.31
0
36
0.18
1
K.ANGIILDLR.N
346 514.3287 1026.6428 1026.6437 -0.85
0
58 0.00054
1
K.TPITITILR.K
421 544.3031 1086.5916 1086.5921 -0.39
1
32
0.69
1
K.KVDEDIQLK.T
498 574.8189 1147.6232 1147.6237 -0.41
0
40
0.12
1
R.AVIIGENTFGK.G
783 704.3685 1406.7224 1406.7227 -0.20
0
(34)
0.65
1
K.MIENENILYIR.V
797 712.3664 1422.7182 1422.7177
0.42
0
51
0.01
1
K.MIENENILYIR.V
1087 863.4725 1724.9304 1724.9309 -0.24
0
31
0.59
1
K.GSVQVILPIDDTEALR.L
1121 886.4509 1770.8872 1770.8887 -0.83
0
45
0.036
1
K.DSALTVISPIEDTPADK.A
39.
gi|47523270
Mass: 46616
alpha-1-antichymotrypsin 2
Query Observed
Mr(expt)
24 360.1913
718.3680
35 367.7258
733.4370
81 410.2141
818.4136
204 465.7718
929.5290
302 499.3110
996.6074
514 580.8394 1159.6642
645 629.3657 1256.7168
664 635.8658 1269.7170
40.
gi|15020816
Mass: 37415
Query
Observed
Mr(expt)
194
460.2672
918.5198
759
689.8755 1377.7364
786
708.3243 1414.6340
1312 1019.0190 2036.0234
1372
710.3526 2128.0360
1414 1094.9870 2187.9594
Score: 293
Queries matched: 10
emPAI: 0.15
[Sus scrofa]
Mr(calc)
718.3683 -0.40
0
28
2.8
1
R.MAAVEAK.L 23
733.4374 -0.51
0
32
0.26
1
K.IVDLFK.K
818.4143 -0.75
0
24
6.8
1
R.VPMMGIR.A
929.5294 -0.38
0
58 0.0026
1
R.SSNLLQLR.L
996.6080 -0.54
1
30
0.34
1
K.LLPETLRR.W
1159.6641
0.12
0
29
0.82
1
R.WIVELYLPK.F
1256.7169 -0.03
0
38
0.081
1
K.VVLVNYIYFK.A
1269.7180 -0.75
0
54 0.0021
1
Score: 288
Queries matched: 8
emPAI:
Mr(calc)
ppm Miss Score
Expect Rank
918.5208 -1.08
0
38
0.17
1
1377.7364
0.01
0
53
0.0047
1
1414.6340
0.05
0
29
0.89
1
2036.0215
0.98
0
69
0.00014
1
2128.0358
0.07
0
26
2.5
1
2187.9597 -0.10
0
74 2.1e-005
1
0.29
Peptide
K.VSVTCLVK.D
K.VNNQALPQPIER.T
K.FNWYMDGVEVR.T
R.SQEPQVYVLAPHPDELSK.S 1311
R.TPEVTCVVVDVSQENPDVK.F 1371
K.YSTTQAQQDSDGSYFLYSK.L
gi|15026997
Mass: 37400
Score: 288
Queries matched: 8
immunoglobulin gamma 1 heavy chain constant region [Equus caballus]
gi|194228715
Mass: 56103
Score: 288
Queries matched: 8
PREDICTED: immunogobulin gamma 1 heavy chain constant region [Equus caballus]
41.
gi|77736341
Mass: 52315
Query Observed
Mr(expt)
184 456.2688
910.5230
250 481.7485
961.4824
505 577.8356 1153.6566
676 641.3093 1280.6040
977 801.9161 1601.8176
1212 939.9437 1877.8728
1226 947.9390 1893.8634
Score: 271
Queries matched: 7
emPAI: 0.13
Mr(calc)
910.5236 -0.61
0
38
0.087
1
R.LPGIVAEGR.S
961.4828 -0.42
0
40
0.11
1
K.SSELVSANR.L
1153.6567 -0.08
1
39
0.079
1
K.SRLPGIVAEGR.S
1280.6037
0.26
0
39
0.31
1
R.SDLYVSDAFHK.A
1601.8161
0.94
1
48
0.02
1
K.LQPLDFKGNAEQSR.L
1877.8717
0.63
0
66 0.00021
1
1893.8666 -1.66
0
(22)
4.6
1
gi|109940161
Mass: 52314
Score: 271
Queries matched: 7
12/9/2009
Page 12 of 20
RecName: Full=Antithrombin-III; Short=ATIII; Flags: Precursor
gi|157838185
Mass: 48618
Score: 271
Queries matched: 7
Chain A, Crystal Structure Of Cleaved Bovine Antithrombin Iii At 3.2 Angstroms Resolution
42.
gi|9858135
Mass: 46904
immunoglobulin G heavy chain
Query
Observed
Mr(expt)
56
396.2165
790.4184
480
567.8129 1133.6112
1127
888.4875 1774.9604
1173
921.4587 1840.9028
1378 1071.5040 2140.9934
1398
717.0554 2148.1444
Score: 251
Queries matched: 12
emPAI: 0.40
[Equus caballus]
Mr(calc)
790.4185 -0.03
0
35
0.35
1
K.LSVETSR.W
1133.6115 -0.18
0
39
0.19
1
K.NTVSVTCLVK.D
1774.9618 -0.74
0
62 0.00038
1
R.VPQVYVLAPHPDELAK.N 1128 1129 1130
1840.9029 -0.05
0
43
0.062
1
K.APDVFPLSICGNTPDPK.V 1172
2140.9953 -0.87
0
37
0.19
1
K.YSTTPAQLNSDGSYFLYSK.L
2148.1442
0.10
0
36
0.22
1
K.CPAPELPGGPSVFIFPPKPK.D 1397 1399
43.
gi|27807517
Mass: 42533
Score: 249
Queries matched: 8
emPAI: 0.45
Query Observed
Mr(expt)
Mr(calc)
182 454.7454
907.4762
907.4763 -0.08
0
37
0.33
2
R.DTQYLLR.T
331 507.7190 1013.4234 1013.4236 -0.19
0
(54) 0.0011
1
R.ADFSGMSSGR.G
349 515.7164 1029.4182 1029.4186 -0.31
0
58 0.00019
1
R.ADFSGMSSGR.G
445 554.2712 1106.5278 1106.5284 -0.52
0
45
0.03
1
K.TYDFLSSFK.D
543 590.3605 1178.7064 1178.7063
0.11
0
50
0.0019
1
R.LVLVNAIYFK.G 542
596 613.3380 1224.6614 1224.6615 -0.03
1
22
5.2
1
R.KHINTWVAEK.T
740 675.8487 1349.6828 1349.6835 -0.52
0
37
0.28
1
K.NVEKPVQMMFK.K
44.
gi|194225324
Mass: 46743
Score: 248
Queries matched: 6
emPAI: 0.23
PREDICTED: similar to alpha-1-antitrypsin; serine protease inhibitor [Equus caballus]
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
234 478.7558
955.4970
955.4975 -0.42
0
30
0.48
1
R.HLEDTLTK.G
317 504.7531 1007.4916 1007.4924 -0.70
0
21
9.8
3
K.QINDYVEK.G
960 799.4217 1596.8288 1596.8300 -0.72
0
86 2.4e-006
1
K.IAPNLADFAFSLYR.H 961 962
1100 879.9593 1757.9040 1757.9047 -0.37
0
111 9.7e-009
1
R.QADLSGITEEAPLTVSK.A
45.
gi|154175408
Mass: 51758
Score: 239
Queries matched: 4
emPAI: 0.28
flagellin B [Campylobacter curvus 525.92]
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
770 697.3441 1392.6736 1392.6741 -0.32
0
58
0.0025
1
R.GAMAVMSIAESAQK.T
1154 902.4575 1802.9004 1802.9010 -0.31
0
118 1.9e-009
1
R.IQTAADDASGLSIADSLR.S
1483 809.0939 2424.2599 2424.2608 -0.40
0
31
0.71
1
1526 890.8057 2669.3953 2669.3984 -1.19
0
32
0.46
1
R.SDLGSVQNQLQATVNNITVTQVNVK.S
46.
gi|18996197
Mass: 35917
Query
Observed
Mr(expt)
480
567.8129 1133.6112
1111
882.4227 1762.8308
1132
889.4305 1776.8464
1323 1021.5260 2041.0374
1356 1056.9910 2111.9674
1372
710.3526 2128.0360
Score: 229
Queries matched: 7
emPAI:
Expect Rank
Mr(calc)
ppm Miss Score
1133.6115 -0.18
0
39
0.19
1
1762.8308
0.01
0
(59)
0.0012
1
1776.8465 -0.01
0
74 3.9e-005
1
2041.0401 -1.32
0
40
0.12
1
2111.9688 -0.62
0
50
0.0076
1
2128.0358
0.07
0
26
2.5
1
0.42
Peptide
K.NTVSVTCLVK.D
R.QSSGLYSLSSMVTVPASSLK.S
K.YSTTPAQLDGDGSYFLYSK.L
R.TPEVTCVVVDVSQENPDVK.F 1371
47.
gi|157165649
Mass: 40295
Score: 226
Queries matched: 5
emPAI: 0.27
Query Observed
Mr(expt)
Mr(calc)
47 385.7078
769.4010
769.4010
0.04
0
23
2.6
1
R.AEFYLK.G
824 727.3666 1452.7186 1452.7184
0.20
0
51
0.0071
1
K.AIYTWVANTMQR.D
849 740.8459 1479.6772 1479.6769
0.22
0
53
0.0036
1
K.EFSYDYVSTELK.888 759.3856 1516.7566 1516.7562
0.29
0
35
0.33
1
K.GYGWIPVDPADVTK.V
898 764.9202 1527.8258 1527.8256
0.14
1
64 0.00036
1
K.ALEIIGNTKGDLER.A
48.
gi|136429
Mass: 24394
Score: 225
Query
Observed
Mr(expt)
Mr(calc)
109
421.7581
841.5016
841.5022
362
523.2853 1044.5560 1044.5564
1422 1106.0560 2210.0974 2210.0967
1450 1142.0940 2282.1734 2282.1729
Queries matched: 11
emPAI: 0.67
ppm Miss Score
Expect Rank
-0.60
0
56
0.0041
1
-0.29
0
37
0.23
1
0.33
0
92 7.3e-007
1
0.25
0
40
0.11
1
Peptide
R.VATVSLPR.S 107 108
K.LSSPATLNSR.V 363
K.IITHPNFNGNTLDNDIMLIK.L 1449
gi|494360
Mass: 23458
Score: 225
Queries matched: 11
gi|999627
Mass: 8814
Score: 225
Queries matched: 11
gi|1942351
Mass: 13284
Score: 225
Queries matched: 11
gi|2914482
Mass: 23460
Score: 225
Queries matched: 11
gi|3318722
Mass: 23457
Score: 225
Queries matched: 11
12/9/2009
Page 13 of 20
gi|110590762
Mass: 23458
Score: 225
Queries matched: 11
gi|157878102
Mass: 24157
Score: 225
Queries matched: 11
gi|242253868
Mass: 25865
Score: 225
Queries matched: 11
49.
gi|1942778
Mass: 15089
Chain A, Deoxy-Haemoglobin
Query Observed
Mr(expt)
361 521.2753 1040.5360
377 529.2728 1056.5310
422 544.3165 1086.6184
495 573.3297 1144.6448
659 634.8583 1267.7020
869 750.3656 1498.7166
gi|146149083
Mass:
gi|146149272
Mass:
50.
Score: 213
Queries matched: 9
emPAI: 0.84
Trapped In The High-Affinity (R) State
Mr(calc)
1040.5365 -0.43
0
(31)
0.58
1
R.MFLGFPTTK.T
1056.5314 -0.35
0
39
0.11
1
R.MFLGFPTTK.T 376
1086.6186 -0.12
1
26
3.6
1
K.LRVDPVNFK.L
1144.6452 -0.28
1
45
0.027
1
-.VLSAADKTNVK.A
1267.7024 -0.26
0
51 0.0047
1
K.FLSSVSTVLTSK.Y
1498.7164
0.13
0
52 0.0064
1
K.VGGHAGEFGAEALER.M 868 870
15220
Score: 213
caballus]
15220
Score: 213
caballus]
gi|15026999
Mass: 37470
Query
Observed
Mr(expt)
71
403.2157
804.4168
84
411.2125
820.4104
194
460.2672
918.5198
894
764.4018 1526.7890
1356 1056.9910 2111.9674
Queries matched: 9
Queries matched: 9
Score: 209
Queries matched: 5
emPAI:
Mr(calc)
ppm Miss Score
Expect Rank
804.4164
0.62
0
(23)
12
1
820.4113 -1.00
0
43
0.12
1
918.5208 -1.08
0
38
0.17
1
1526.7875
1.00
0
78 1.5e-005
1
2111.9688 -0.62
0
50
0.0076
1
0.18
Peptide
K.DALMISR.T
K.DALMISR.T
K.VSVTCLVK.D
K.CSVTNVGVPQPISR.A
K.YSTTPAQLDGDGSYFLYSK.L
51.
gi|194038345
Mass: 38368
Score: 209
Queries matched: 7
emPAI: 0.18
Query Observed
Mr(expt)
Mr(calc)
468 561.3135 1120.6124 1120.6128 -0.33
0
64 0.00037
1
R.DTQSIIFLGK.V
664 635.8658 1269.7170 1269.7180 -0.75
0
54
0.0021
1
785 706.8826 1411.7506 1411.7500
0.46
0
33
0.43
1
R.FDSPFLFATVLR.D
800 475.6156 1423.8250 1423.8259 -0.66
0
36
0.067
1
R.TLNRPSNLLQLR.L 802
1432 746.6866 2237.0380 2237.0389 -0.41
0
21
6.5
1
R.ELYHSEAFPTNFQDLEAAR.R
52.
gi|158605017
Mass: 30046
Score: 205
Queries matched: 7
emPAI: 0.23
Query Observed
Mr(expt)
Mr(calc)
61 398.2034
794.3922
794.3923 -0.05
0
21
13
1
K.GVTFTDR.G
316 504.2738 1006.5330 1006.5335 -0.44
1
32
0.89
1
K.VEKEFVEK.G
399 537.8006 1073.5866 1073.5869 -0.28
0
32
1.2
1
K.WTAVGTGAALK.L
709 657.8389 1313.6632 1313.6615
1.31
1
36
0.21
1
R.GTYIKYEANQK.G
965 799.8978 1597.7810 1597.7810
0.02
0
(55) 0.0032
1
R.NAVMYNDFVVIADK.S
971 800.9384 1599.8622 1599.8620
0.13
0
26
2
1
K.LLDKPLFTPDANTR.K
982 807.8951 1613.7756 1613.7759 -0.18
0
58 0.0016
1
R.NAVMYNDFVVIADK.S
53.
gi|157165083
Mass: 50030
Score: 202
Queries matched: 6
emPAI: 0.21
transcription termination factor Rho [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
384 529.3032 1056.5918 1056.5928 -0.88
0
58 0.0025
1
R.VGDIITGQVR.E 385
437 551.8284 1101.6422 1101.6434 -1.05
0
49 0.0078
1
R.VLDLFTPIGK.G
685 644.3435 1286.6724 1286.6718
0.54
1
23
6.1
2
K.DNKELLSILNE.749 682.3418 1362.6690 1362.6701 -0.74
0
28
1.6
1
R.SAIATMDDVEALK.F
815 717.8685 1433.7224 1433.7224
0.03
0
44
0.049
1
K.IEAVNYMPLADAK.E
54.
gi|122411
Mass: 15236
Score: 194
Queries matched: 8
emPAI: 0.22
Query Observed
Mr(expt)
Mr(calc)
361 521.2753 1040.5360 1040.5365 -0.43
0
(31)
0.58
1
R.MFLGFPTTK.T
377 529.2728 1056.5310 1056.5314 -0.35
0
39
0.11
1
R.MFLGFPTTK.T 376
422 544.3165 1086.6184 1086.6186 -0.12
1
26
3.6
1
K.LRVDPVNFK.L
495 573.3297 1144.6448 1144.6452 -0.28
1
45
0.027
1
M.VLSAADKTNVK.A
659 634.8583 1267.7020 1267.7024 -0.26
0
51 0.0047
1
K.FLSSVSTVLTSK.Y
880 758.3632 1514.7118 1514.7113
0.33
0
33
0.42
1
K.VGGHAGEYGAEALER.M 879
gi|230479
Mass: 15105
Score: 194
Queries matched: 8
gi|230632
Mass: 15105
Score: 194
Queries matched: 8
gi|313500
Mass: 15236
Score: 194
Queries matched: 8
gi|24158717
Mass: 15105
Score: 194
Queries matched: 8
12/9/2009
Page 14 of 20
55.
gi|157164344
Mass: 49258
Score: 193
Queries matched: 5
emPAI: 0.30
biotin carboxylase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
511 580.3143 1158.6140 1158.6132
0.74
1
20
14
1
R.VVEKEADLEK.A
662 635.3923 1268.7700 1268.7704 -0.25
0
42
0.01
1
K.VALDQLIISGIK.T
838 735.4091 1468.8036 1468.8024
0.83
0
43
0.04
1
K.LIEESPAILLDEK.T
902 769.3909 1536.7672 1536.7680 -0.49
0
34
0.46
1
K.FIGPSVAAMALMSDK.S
921 777.4122 1552.8098 1552.8097
0.09
0
53 0.0041
1
R.AGVPVIPGSDGAVADTK.A
56.
gi|154175542
Mass: 22096
Score: 180
Queries matched: 5
emPAI: 0.33
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
340 511.2722 1020.5298 1020.5314 -1.52
0
27
1.7
3
R.FPIVADMTK.S 339
574 602.8435 1203.6724 1203.6724
0.04
0
34
0.34
1
R.HAVINDLPLGR.N
702 654.3511 1306.6876 1306.6881 -0.37
1
36
0.28
1
R.GSFLLDKDGTVR.H
843 737.8987 1473.7828 1473.7827
0.07
0
84 5.1e-006
1
R.GFDVLLEDAGVALR.G
Mass: 22011
Score: 180
Queries matched: 5
gi|157164012
57.
58.
gi|157165273
Mass: 40059
Score:
Query Observed
Mr(expt)
Mr(calc)
258 486.7551
971.4956
971.4964
471 563.7837 1125.5528 1125.5529
487 571.7808 1141.5470 1141.5478
503 576.3080 1150.6014 1150.6022
699 653.3384 1304.6622 1304.6612
792 710.9010 1419.7874 1419.7874
gi|122614
Mass: 15998
Score: 163
Query
Observed
Mr(expt)
Mr(calc)
501
575.3405 1148.6664 1148.6666
670
637.8658 1273.7170 1273.7183
803
713.8458 1425.6770 1425.6776
1292 1000.4650 1998.9154 1998.9146
1299 1008.4620 2014.9094 2014.9095
167
Queries matched: 6
emPAI: 0.08
-0.74
0
28
1.2
1
-0.01
0
43
0.067
1
-0.64
0
(36)
0.21
1
-0.70
0
28
2
1
0.81
1
44
0.06
1
0.02
1
24
2.7
1
Peptide
K.ISDYAYIK.T
K.SPFAMLDFAK.S
K.SPFAMLDFAK.S
R.IPGFGGEIYAK.L
K.NAYEKLPDDLK.A
K.IRIPGFGGEIYAK.L
Queries matched: 6
emPAI: 0.78
-0.11
0
34
0.15
1
K.VVAGVANALAHK.Y
-0.98
0
23
4.1
1
R.LLVVYPWTQR.F 671
-0.39
0
40
0.099
1
K.DFTPELQASYQK.V
0.43
0
67 0.00017
1
-0.03
0
(66) 0.00018
1
Mass: 15970
Score: 163
Queries matched: 6
gi|230480
Mass: 16129
Score: 163
Queries matched: 6
gi|255683515
59.
gi|291470
Mass: 19537
Query Observed
Mr(expt)
351 516.2612 1030.5078
509 579.7767 1157.5388
845 738.8678 1475.7210
Score: 163
Mr(calc)
1030.5083
1157.5387
1475.7216
Queries matched: 3
emPAI: 0.37
ppm Miss Score
Expect Rank
-0.47
0
59
0.0019
1
0.16
0
24
2.9
1
-0.40
0
80 1.1e-005
1
Peptide
K.YAASSYLTR.T
K.SYSSVSCQVK.H
K.VNDAVTTDGVQTTR.S
gi|291474
Mass: 17423
Score: 163
Queries matched: 3
gi|291478
Mass: 22654
Score: 163
Queries matched: 3
gi|9858133
Mass: 24186
Score: 163
Queries matched: 3
gi|194214037
Mass: 17257
Score: 163
Queries matched: 3
gi|194214039
Mass: 15452
Score: 163
Queries matched: 3
60.
gi|2232299
Mass: 47885
Score: 163
Queries matched: 7
emPAI: 0.14
Query Observed
Mr(expt)
Mr(calc)
447 554.2889 1106.5632 1106.5641 -0.81
0
28
2.3
1
R.ESASVTCLVK.G
655 633.8090 1265.6034 1265.6040 -0.46
0
47
0.026
1
K.ALETHTYFER.H
996 817.4280 1632.8414 1632.8413
0.10
0
42
0.083
1
K.GFAPADVFVQWLQR.G 995
1133 892.4922 1782.9698 1782.9702 -0.21
0
46
0.016
1
K.DVAMKPPSVYLLPPTR.E 1134
1151 900.4901 1798.9656 1798.9651
0.29
0
(29)
1.1
1
gi|3834667
Mass: 48183
Score: 163
Queries matched: 7
gi|24496448
Mass: 65621
Score: 163
Queries matched: 7
12/9/2009
immunoglobulin
gi|33413902
immunoglobulin
gi|34538498
immunoglobulin
Page 15 of 20
Mass: 49106
Score: 163
Queries matched: 7
Mass: 49909
Score: 163
Queries matched: 7
heavy chain constant region [Bos taurus]
61.
gi|157165135
Mass: 62239
Score: 157
Queries matched: 3
emPAI: 0.17
ComEC/Rec2 family protein [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
661 635.3272 1268.6398 1268.6401 -0.19
0
61 0.00092
1
K.ATEFNYLQVGK.D
719 664.8095 1327.6044 1327.6044
0.01
0
45
0.025
1
K.ATLDAYQDEFR.I
730 671.8539 1341.6932 1341.6928
0.32
0
51
0.0082
1
R.YTLAQSNYIAAK.N
62.
gi|167621418
Mass: 46913
Score: 154
Queries matched: 7
emPAI: 0.23
alpha-1-antitrypsin [Equus caballus]
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
37 375.7054
749.3962
749.3959
0.40
0
22
5.5
1
K.FLEDVK.K
317 504.7531 1007.4916 1007.4924 -0.70
0
21
9.8
3
K.QINDYVEK.G
960 799.4217 1596.8288 1596.8300 -0.72
0
86 2.4e-006
1
K.IAPNLADFAFSLYR.H 961 962
1136 893.9758 1785.9370 1785.9360
0.58
0
25
3.4
1
R.QADLSGITEEVPLTVSK.A 1135
Mass: 46945
Score: 154
gi|197631751
Mass: 46973
Score: 154
gi|197631755
Mass: 46835
Score: 154
gi|197631757
gi|197631761
Mass: 46791
Score: 154
Queries matched: 7
Queries matched: 7
Queries matched: 7
Queries matched: 7
63.
gi|157163930
Mass: 45716
Score:
Query Observed
Mr(expt)
Mr(calc)
271 489.7535
977.4924
977.4930
582 606.7800 1211.5454 1211.5458
663 635.8456 1269.6766 1269.6757
143
Queries matched: 3
emPAI: 0.07
concisus 13826]
-0.61
0
43
0.049
1
K.NVDLSGFAR.Y
-0.32
0
47
0.017
1
R.NEWSLDASYK.Y
0.72
0
53 0.0055
1
K.LTFLSWYAAAK.D
64.
gi|157165008
transketolase A
Query Observed
274 491.2837
948 793.9304
985 810.4447
65.
gi|223041213
Mass: 30058
Score: 140
Queries matched: 5
extracellular tungstate binding protein [Campylobacter rectus RM3267]
Query Observed
Mr(expt)
Mr(calc)
61 398.2034
794.3922
794.3923 -0.05
0
21
13
1
K.GVTFTDR.G
316 504.2738 1006.5330 1006.5335 -0.44
1
32
0.89
1
K.VEKEFVEK.G
399 537.8006 1073.5866 1073.5869 -0.28
0
32
1.2
1
K.WTAVGTGAALK.L
971 800.9384 1599.8622 1599.8620
0.13
0
26
2
1
K.LLDKPLFTPDANTR.K
1157 907.9374 1813.8602 1813.8669 -3.66
0
29
1.2
1
K.LGENCDADILFVHSPK.V
Mass: 44829
Score: 142
Queries matched: 3
emPAI: 0.07
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
980.5528
980.5542 -1.41
0
32
0.32
1
K.EPVPAEVLK.A
1585.8462 1585.8464 -0.09
0
78 1.6e-005
1
K.ALAPTFGGINLEDIR.A
1618.8748 1618.8753 -0.26
0
32
0.45
1
K.GADMFLGLSKPGVVTK.E
Mass: 30100
Score: 140
Queries matched: 5
gi|255322100
extracellular tungstate binding protein [Campylobacter showae RM3277]
66.
gi|157164830
Mass: 38482
Score: 139
Queries matched: 5
emPAI: 0.18
Query Observed
Mr(expt)
Mr(calc)
269 489.7497
977.4848
977.4852 -0.34
0
(32)
1
1
R.SITDMQGVK.V
295 497.7480
993.4814
993.4801
1.35
0
33
0.44
1
R.SITDMQGVK.V
420 543.8054 1085.5962 1085.5968 -0.52
0
29
1.3
1
K.DLQIEELVK.L
612 619.3382 1236.6618 1236.6615
0.28
0
45
0.033
1
K.NGFSAVNLIFR.D
632 624.3950 1246.7754 1246.7761 -0.54
0
32
0.028
1
K.VLHLLGGANLLK.V
67.
gi|223039637
Mass: 39873
Score: 134
Queries matched: 3
emPAI: 0.17
Query Observed
Mr(expt)
Mr(calc)
47 385.7078
769.4010
769.4010
0.04
0
23
2.6
1
R.AEFYLK.G
714 661.3064 1320.5982 1320.5980
0.18
0
59 0.00087
1
R.DNSVIGCGTGDVK.A
824 727.3666 1452.7186 1452.7184
0.20
0
51
0.0071
1
K.AIYTWVANTMQR.D
68.
gi|157165368
Mass: 53010
Score: 133
Queries matched: 2
emPAI: 0.13
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
441 553.7828 1105.5510 1105.5516 -0.52
0
54
0.0057
1
K.GFNDVAQSLR.I
942 790.3578 1578.7010 1578.7009
0.08
0
80 6.6e-006
1
K.SLADDDIQNTTGDSK.F
12/9/2009
69.
Page 16 of 20
gi|114051856
Mass: 51546
Score: 125
Queries matched: 2
emPAI: 0.06
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
430 547.2671 1092.5196 1092.5199 -0.27
0
37
0.31
1
K.AQYEEIANR.S
697 651.8611 1301.7076 1301.7078 -0.14
0
88 1.8e-006
1
R.SLDLDSIIAEVK.A
Mass: 59000
Score: 125
Queries matched: 2
gi|116004057
gi|149714762
Mass: 62397
Score: 125
Queries matched: 2
PREDICTED: similar to keratin 5 isoform 1 [Equus caballus]
70.
gi|157165286
Mass: 58599
Score: 122
Queries matched: 4
Query Observed
Mr(expt)
Mr(calc)
78 407.2452
812.4758
812.4756
0.32
0
35
0.24
1
806 715.3458 1428.6770 1428.6772 -0.14
1
35
0.33
1
1021 836.3907 1670.7668 1670.7650
1.10
0
30
0.82
1
1206 936.5345 1871.0544 1871.0557 -0.65
0
23
1.4
1
Peptide
K.INQPITK.E
R.YEDVKEGVEYAK.A
K.EQAMYYFLSGYTAK.V
K.NIIFLSADAFGVLPPVAK.L
71.
gi|157165527
Mass: 43876
Score: 122
Queries matched: 3
emPAI: 0.08
Query Observed
Mr(expt)
Mr(calc)
205 467.2267
932.4388
932.4392 -0.37
0
26
3.1
1
R.FAYQYNK.K
271 489.7535
977.4924
977.4930 -0.61
0
43
0.049
1
K.NVDLSGFAR.Y
990 816.3725 1630.7304 1630.7304
0.04
0
52 0.0037
1
K.GNFFYATSAYTFDK.F
72.
gi|27807349
Mass: 51691
Query Observed
Mr(expt)
375 528.2611 1054.5076
458 556.8031 1111.5916
1030 839.9354 1677.8562
Score: 122
Queries matched: 4
emPAI: 0.13
Mr(calc)
1054.5084 -0.68
0
29
2
1
K.YPVASFTDR.T
1111.5914
0.26
0
32
0.61
1
R.LYQDFSVLK.K
1677.8573 -0.65
0
61 0.0012
1
R.SAEAVLGEALTDFSLR.L 1031
73.
gi|157165191
Mass: 45841
adenylosuccinate synthetase
Query Observed
Mr(expt)
449 554.7851 1107.5556
595 613.3102 1224.6058
603 617.3394 1232.6642
Score: 118
Queries matched: 3
emPAI: 0.07
Mr(calc)
1107.5560 -0.34
0
43
0.074
1
K.VGYISTSPER.S
1224.6060 -0.14
0
36
0.27
1
R.LDGVDTYALMK.L
1232.6653 -0.83
0
40
0.099
1
K.LDVLDGFEVVK.I
74.
gi|18999435
Mass: 62340
Score: 115
Queries matched: 3
emPAI: 0.05
Query Observed
Mr(expt)
Mr(calc)
430 547.2671 1092.5196 1092.5199 -0.27
0
37
0.31
1
K.AQYEEIANR.S
454 556.2897 1110.5648 1110.5669 -1.88
0
25
2.3
1
R.ISISTSGGSFR.N
720 665.3666 1328.7186 1328.7187 -0.05
0
53 0.0051
1
R.NLDLDSIIAEVK.A
gi|109096779
Mass: 60910
Score: 115
Queries matched: 3
PREDICTED: keratin 5 isoform 8 [Macaca mulatta]
gi|109096789
Mass: 62567
Score: 115
Queries matched: 3
gi|109096793
Mass: 58646
Score: 115
Queries matched: 3
gi|109096795
Mass: 60935
Score: 115
Queries matched: 3
gi|109096797
Mass: 61633
Score: 115
Queries matched: 3
gi|109096803
Mass: 59714
Score: 115
Queries matched: 3
gi|109096805
Mass: 53898
Score: 115
Queries matched: 3
gi|109096807
Mass: 52644
Score: 115
Queries matched: 3
Mass: 51539
Score: 115
Queries matched: 3
gi|109096809
gi|119395754
Mass: 62340
Score: 115
Queries matched: 3
gi|119617041
Mass: 59397
Score: 115
Queries matched: 3
keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), isoform CRA_d [Homo sapiens]
75.
gi|157165109
Mass: 35585
Score: 111
Queries matched: 4
emPAI: 0.09
Query Observed
Mr(expt)
Mr(calc)
310 501.3248 1000.6350 1000.6355 -0.40
0
45 0.0012
1
R.VLLMLALTK.T 309
374 527.3185 1052.6224 1052.6230 -0.50
0
30
0.2
1
K.LIAAVPDINK.I
0
37
0.2
1
K.IGTVFYGNVK.Y
433 549.3029 1096.5912 1096.5917 -0.42
12/9/2009
Page 17 of 20
76.
gi|172048405
Mass: 59490
Score: 108
Queries matched: 2
emPAI: 0.11
RecName: Full=Formate--tetrahydrofolate ligase; AltName: Full=Formyltetrahydrofolate synthetase; Short=FHS; Short=FTHFS
Query Observed
Mr(expt)
Mr(calc)
506 578.3525 1154.6904 1154.6910 -0.51
0
60 0.00032
1
K.GALELAELVLK.A
1026 837.4586 1672.9026 1672.9036 -0.56
0
48
0.013
1
K.LILVTATNPTPYGEGK.T
77.
gi|157164634
Mass: 48581
Score: 106
Queries matched: 3
emPAI: 0.14
Query Observed
Mr(expt)
Mr(calc)
358 516.7957 1031.5768 1031.5764
0.46
0
25
4.5
5
K.ADAFLVALGR.V
1005 824.9361 1647.8576 1647.8580 -0.23
0
42
0.063
1
R.LQTNVSNIYAVGDVR.G
1236 952.4556 1902.8966 1902.8999 -1.73
0
39
0.13
1
K.EAYAPFEITNSNVFSSK.T
78.
gi|157164859
Mass: 27476
Score: 101
Queries matched: 2
emPAI: 0.12
Query Observed
Mr(expt)
Mr(calc)
566 601.3244 1200.6342 1200.6350 -0.61
0
31
0.9
1
K.GNTELLEAINK.A
978 803.3961 1604.7776 1604.7794 -1.12
0
70 0.00016
1
K.ADVVFNQVSINEDR.K
79.
gi|157165087
Mass: 57228
Score: 95
Queries matched: 3
emPAI: 0.06
D-3-phosphoglycerate dehydrogenase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
916 774.3588 1546.7030 1546.7046 -1.00
0
25
2.3
1
R.AGVGVDNVDIDGCSR.R
923 778.3851 1554.7556 1554.7565 -0.58
0
34
0.47
1
R.GGLYNEEALYEGLK.S
932 784.4436 1566.8726 1566.8729 -0.17
0
36
0.13
2
R.NIAVEAVEQAILAAR.G
80.
gi|27807209
Mass: 54676
Score: 94
Queries matched: 3
emPAI: 0.06
alpha-2-plasmin inhibitor [Bos taurus]
Query Observed
Mr(expt)
Mr(calc)
307 501.2540 1000.4934 1000.4937 -0.30
0
64 0.0003
1
K.GEDLANINR.W
576 603.3171 1204.6196 1204.6200 -0.31
1
30
1.3
1
R.SKFDPNLTQR.G 575
81.
gi|157165398
Mass: 45766
Score: 92
Queries matched: 2
Query Observed
Mr(expt)
Mr(calc)
336 509.2823 1016.5500 1016.5502 -0.15
0
43
0.07
2
757 687.3580 1372.7014 1372.7020 -0.41
0
49
0.014
1
Peptide
R.AIESITLDR.G
K.MSPAEILTELNR.L
82.
gi|157164740
Mass: 36430
Score: 83
Queries matched: 2
emPAI: 0.19
Query Observed
Mr(expt)
Mr(calc)
700 653.3562 1304.6978 1304.6976
0.19
0
51 0.0093
1
K.AVAEVLAGYGVEK.A
1049 845.9726 1689.9306 1689.9301
0.32
0
32
0.26
1
83.
gi|157164819
Mass: 50763
Score: 82
Queries matched: 3
beta ketoacyl-acyl carrier protein synthase II [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
40 381.7068
761.3990
761.3993 -0.32
0
21
8.6
1
K.MLEELK.A
248 481.2728
960.5310
960.5314 -0.32
1
29
1.1
2
K.MLEELKAK.N
822 722.3481 1442.6816 1442.6824 -0.50
0
32
0.65
1
K.DSNNHVSMLVSPK.T
84.
gi|229552
Mass: 66088
albumin
Query Observed
Mr(expt)
115 424.2554
846.4962
519 582.3187 1162.6228
Score: 81
Mr(calc)
846.4963
1162.6234
Queries matched: 2
-0.09
1
40
0.062
1
-0.45
0
42
0.073
1
Peptide
R.LSQKFPK.A
K.LVNELTEFAK.T
gi|1351907
Mass: 69248
Score: 81
Queries matched: 2
Mass: 69278
Score: 81
Queries matched: 2
gi|30794280
gi|74267962
Mass: 69190
Score: 81
Queries matched: 2
Mass: 69248
Score: 81
Queries matched: 2
gi|154425704
85.
86.
gi|157164833
phosphopyruvate
Query Observed
558 597.8036
941 788.9142
Mass: 45147
Score: 78
Queries matched: 2
hydratase [Campylobacter concisus 13826]
Mr(expt)
Mr(calc)
1193.5926 1193.5928 -0.12
0
29
1.6
1
1575.8138 1575.8145 -0.38
0
50
0.015
1
gi|255322858
Mass: 52136
Score: 73
Queries matched: 2
Peptide
K.EAGYELGSQIK.L
K.VQLVGDDLFVTNEK.I
emPAI: 0.06
12/9/2009
flagellin B [Campylobacter showae RM3277]
Query Observed
Mr(expt)
Mr(calc)
419 543.3010 1084.5874 1084.5876 -0.18
0
42
0.074
1
1483 809.0939 2424.2599 2424.2608 -0.40
0
31
0.71
1
87.
Page 18 of 20
Peptide
K.GVGALAENINK.V
gi|123506
Mass: 8353
Score: 68
Queries matched: 2
Query Observed
Mr(expt)
Mr(calc)
278 492.3032
982.5918
982.5923 -0.49
0
43
0.016
1
R.ALGILNLNR.N
523 583.7666 1165.5186 1165.5186
0.05
0
25
2.3
1
R.SYPSNATCPR.T
gi|5019730
Mass: 12474
Score: 68
Queries matched: 2
gi|76782253
Mass: 12343
Score: 68
Queries matched: 2
88.
gi|157164553
Mass: 66898
Score: 68
Queries matched: 2
emPAI: 0.05
Query Observed
Mr(expt)
Mr(calc)
684 643.8696 1285.7246 1285.7241
0.40
1
28
1.4
1
K.INEVIKDAGLSK.S
925 778.4152 1554.8158 1554.8154
0.28
0
40
0.094
1
K.SLGNFNLEGIPAAPR.G
89.
gi|157164319
Mass: 38441
Score: 66
Queries matched: 2
Query Observed
Mr(expt)
Mr(calc)
643 629.3254 1256.6362 1256.6361
0.14
0
29
1.3
1
1013 826.9094 1651.8042 1651.8053 -0.64
0
37
0.26
1
Peptide
K.DTTAHIQSSGIK.T
K.ATISEINSETDFVAR.N
gi|171855138
Mass: 39114
Score: 66
Queries matched: 2
90.
gi|154175426
Mass: 28890
Score: 66
Queries matched: 2
Query Observed
Mr(expt)
Mr(calc)
256 485.2898
968.5650
968.5655 -0.43
0
36
0.16
1
R.VNAISAGPIK.T
854 746.4064 1490.7982 1490.7981
0.12
0
30
0.79
1
K.EGGSVLTLTYLGGPK.F
gi|157165165
Mass: 29118
Score: 66
Queries matched: 2
gi|223038888
Mass: 29342
Score: 66
Queries matched: 2
gi|255323509
Mass: 29040
Score: 66
Queries matched: 2
91.
gi|157164353
Mass: 66046
Score: 62
Queries matched: 2
succinate dehydrogenase flavoprotein subunit [Campylobacter concisus
Query Observed
Mr(expt)
Mr(calc)
485 569.3172 1136.6198 1136.6190
0.77
0
42
0.047
1
713 660.8427 1319.6708 1319.6721 -0.96
0
20
17
2
13826]
Peptide
K.SLVIATGGYTR.I
K.GAAYLADQDAVVK.F
92.
gi|158604964
Mass: 56612
Score: 60
Queries matched: 2
aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (Asp/Glu-ADT subunit B) [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
848 739.8979 1477.7812 1477.7816 -0.27
0
26
2.1
1
R.YFEELIAAGIQPK.L
979 805.4432 1608.8718 1608.8723 -0.26
0
34
0.32
1
R.AGTPLLEIVSEPDLR.S
93.
gi|157165180
Mass: 20941
Score: 60
Queries matched: 1
emPAI: 0.16
Query Observed
Mr(expt)
Mr(calc)
1089 863.9705 1725.9264 1725.9261
0.20
0
60 0.0007
1
94.
gi|157164241
Mass: 54307
Score: 60
Queries matched: 2
emPAI: 0.06
2-isopropylmalate synthase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
667 636.8304 1271.6462 1271.6470 -0.57
0
22
7.1
1
R.QVEGTINGIGER.A
807 715.3901 1428.7656 1428.7646
0.73
0
39
0.12
1
R.AGNAALEEIVMAIK.T
95.
gi|154173809
Mass: 46458
Score: 59
Queries matched: 2
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
628 623.3655 1244.7164 1244.7129
2.87
0
21
4.1
1
K.TIVSGGLNIFPK.W
706 657.3403 1312.6660 1312.6663 -0.22
0
39
0.14
1
R.FSSDGQSVVFIK.E
12/9/2009
Page 19 of 20
gi|157164610
Mass: 46159
Score: 59
Queries matched: 2
96.
gi|42560938
Mass: 75446
Score: 58
Queries matched: 2
Na+ ABC transporter, ATP-binding component [Mycoplasma mycoides subsp. mycoides SC str. PG1]
59 396.7159 791.4172 791.4177 -0.62
0
33
0.63
1
K.ILDEFR.R
222 474.7665 947.5184 947.5188 -0.42
1
25
4.3
1
K.ILDEFRR.K
gi|67920112
Mass: 69214
Score: 58
Queries matched: 2
unknown protein [Crocosphaera watsonii WH 8501]
gi|78186810
Mass: 89410
Score: 58
Queries matched: 2
primosomal protein n [Pelodictyon luteolum DSM 273]
gi|193212911
Mass: 39632
Score: 58
Queries matched: 2
histidinol-phosphate aminotransferase [Chlorobaculum parvum NCIB 8327]
gi|83319333
Mass: 71544
Score: 56
Queries matched: 2
ABC transporter, ATP-binding protein, putative [Mycoplasma capricolum subsp. capricolum ATCC 27343]
97.
gi|157165609
Mass: 54107
Score: 57
Queries matched: 1
emPAI: 0.06
glutamine synthetase, type I [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
1037 840.9330 1679.8514 1679.8519 -0.25
0
57 0.0024
1
K.FGNLVEAADNVQIYK.Y
98.
gi|154148662
Mass: 52006
Score: 56
Queries matched: 2
phosphomannomutase/phosphoglucomutase (PMM/PGM) [Campylobacter hominis ATCC BAA-381]
Query Observed
Mr(expt)
Mr(calc)
348 515.2998 1028.5850 1028.5866 -1.50
0
27
2.5
4
K.EIITIDGIR.I
510 579.8276 1157.6406 1157.6404
0.18
0
29
1.7
1
R.ASNTTPVIVTR.F
99.
gi|157163909
Mass: 45295
Score: 54
Queries matched: 1
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
1091 865.4522 1728.8898 1728.8894
0.28
0
54 0.0043
1
K.IADVLSDINNASLQEK.I
100.
gi|16329620
Mass: 93153
Score: 54
Queries matched: 2
emPAI: 0.04
methyl-accepting chemotaxis protein [Synechocystis sp. PCC 6803]
236 478.7977 955.5808 955.5815 -0.65
0
54 0.0051
1
R.TVGGILQIR.E 237
101.
gi|225632
Mass: 24420
casein alphaS1
Query Observed
Mr(expt)
657 634.3564 1266.6982
764 692.8680 1383.7214
Score: 52
Mr(calc)
1266.6972
1383.7227
Queries matched: 3
0.84
0
21
5.6
1
-0.93
0
31
0.86
1
Peptide
R.YLGYLEQLLR.L
R.FFVAPFPEVFGK.E 765
Mass: 24513
Score: 52
Queries matched: 3
gi|30794348
Mass: 24383
Score: 52
Queries matched: 3
gi|70905149
gi|75038951
Mass: 24311
Score: 52
Queries matched: 3
gi|159793187
Mass: 13839
Score: 52
Queries matched: 3
gi|159793191
Mass: 23598
Score: 52
Queries matched: 3
Mass: 18671
Score: 52
Queries matched: 3
gi|159793193
gi|159793197
Mass: 23473
Score: 52
Queries matched: 3
gi|159793209
Mass: 22442
Score: 52
Queries matched: 3
gi|195973860
Mass: 24427
Score: 52
Queries matched: 3
102.
gi|157347169
unnamed protein
Query Observed
290 494.7948
Mass: 30214
Score: 52
Queries matched: 1
emPAI: 0.11
product [Vitis vinifera]
Mr(expt) Mr(calc)
987.5750 987.5753 -0.26
0
52 0.0087
1
K.LDVLYPIR.G
gi|225450829
Mass: 29623
Score: 52
Queries matched: 1
PREDICTED: hypothetical protein [Vitis vinifera]
103.
gi|16755594
Mass: 55475
Score: 50
Queries matched: 1
mucin [Bos taurus]
Query Observed
Mr(expt)
Mr(calc)
478 566.3137 1130.6128 1130.6124
0.37
0
50
0.013
1
Peptide
R.VSLYFLSFR.I
12/9/2009
Page 20 of 20
gi|41386778
Mass: 58056
Score: 50
Queries matched: 1
mucin 1 [Bos taurus]
Mass: 33390
Score: 50
Queries matched: 1
gi|83715957
mucin VNTR11 [Bos taurus]
104.
gi|157164015
Mass: 43676
Score: 50
Queries matched: 1
emPAI: 0.08
trimethylamine-n-oxide reductase 1 (tmaoreductase 1; trimethylamine oxidase 1) [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
951 795.9115 1589.8084 1589.8089 -0.31
0
50
0.012
1
R.VLINTWFSQEEPK.I
105.
gi|118475424
Mass: 59542
Score: 50
Queries matched: 1
dihydroxy-acid dehydratase [Campylobacter fetus subsp. fetus 82-40]
Query Observed
Mr(expt)
Mr(calc)
644 629.3433 1256.6720 1256.6725 -0.34
0
50
0.04
1
R.GLGADVALITDGR.F
Mass: 59387
Score: 50
Queries matched: 1
gi|154148470
dihydroxy-acid dehydratase [Campylobacter hominis ATCC BAA-381]
gi|154175263
Mass: 59869
Score: 50
Queries matched: 1
dihydroxy-acid dehydratase [Campylobacter curvus 525.92]
gi|157165359
Mass: 62637
Score: 50
Queries matched: 1
dihydroxy-acid dehydratase [Campylobacter concisus 13826]
gi|223038459
Mass: 59660
Score: 50
Queries matched: 1
dihydroxy-acid dehydratase [Campylobacter rectus RM3267]
Mass: 59726
Score: 50
Queries matched: 1
gi|255323592
dihydroxy-acid dehydratase [Campylobacter showae RM3277]
106.
gi|6320157
Mass: 19051
Score: 49
Queries matched: 2
emPAI: 0.18
Query Observed
Mr(expt)
Mr(calc)
608 617.8558 1233.6970 1233.6969
0.13
0
49 0.0074
1
K.IVEIPGEVPPGK.Y 607
gi|151941952
Mass: 19021
Score: 49
Queries matched: 2
gi|190405057
Mass: 19007
Score: 49
Queries matched: 2
107.
gi|157164517
Mass: 96149
methyl-accepting chemotaxis
Query Observed
Mr(expt)
496 573.3318 1144.6490
567 601.3425 1200.6704
Score: 49
Queries matched: 2
protein [Campylobacter concisus 13826]
Mr(calc)
1144.6492 -0.12
0
21
9.1
1
1200.6714 -0.77
1
28
1.2
1
Peptide
K.AGALFLIPESK.S
R.GLSEKDVLAAAK.T
12/9/2009
Page 1 of 20
User
:
Email
:
Search title
:
MS data file
:
Database
:
Timestamp
:
Enzyme
:
Mass values
:
Protein Mass
:
:
Instrument type
:
Number of queries
:
Protein hits
:
LTQFT Daemon
N_18_7_09_12.RAW relex2
D:\Data\Mark\18_7-09\N_18_7_09_12.RAW
9 Dec 2009 at 04:34:32 GMT
Trypsin
Monoisotopic
Unrestricted
± 4 ppm
± 0.4 Da
1
ESI-TRAP
1522
gi|76363596 RecName: Full=Serum albumin; Flags: Precursor
gi|126723507 preproalbumin [Equus caballus]
gi|157164553 molecular chaperone DnaK [Campylobacter concisus 13826]
gi|157165471 radical SAM domain-containing protein [Campylobacter concisus 13826]
gi|7331218
gi|28317
gi|154175431 molecular chaperone DnaK [Campylobacter curvus 525.92]
gi|189027839 RecName: Full=Elongation factor G; Short=EF-G
gi|157165732 nitrate reductase [Campylobacter concisus 13826]
gi|255322039 translation elongation factor G [Campylobacter showae RM3277]
gi|435476
cytokeratin 9 [Homo sapiens]
gi|157165097 chaperonin GroEL [Campylobacter concisus 13826]
gi|157165518 elongation factor P (EF-P) [Campylobacter concisus 13826]
gi|194038349 PREDICTED: similar to SERPINA3-6 [Sus scrofa]
gi|2492797
gi|31340900 serpin peptidase inhibitor, clade A, member 3 [Bos taurus]
gi|181402
gi|51831151 immunoglobulin mu heavy chain constant chain secreted form [Equus caballus]
gi|229552
albumin
gi|157164952 arylsulfotransferase [Campylobacter concisus 13826]
gi|157164517 methyl-accepting chemotaxis protein [Campylobacter concisus 13826]
gi|122614
gi|122411
gi|136429
gi|1942778
gi|126352628 transferrin [Equus caballus]
gi|291470
gi|158604891 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase [Campylobacter concisus 13826]
gi|27807349 serpin peptidase inhibitor, clade G, member 1 [Bos taurus]
gi|123506
gi|2232299
gi|194216172 PREDICTED: similar to Alpha-1B-glycoprotein precursor (Alpha-1-B glycoprotein) [Equus caballus]
gi|157165065 tRNA synthetase, class II [Campylobacter concisus 13826]
12/9/2009
gi|154175542
gi|157164319
gi|9858135
gi|157163930
gi|18996197
gi|157164029
gi|77736341
gi|27806941
gi|6320157
gi|157165623
gi|157164353
gi|114051856
gi|154175347
gi|157164740
gi|157165180
gi|157164144
gi|149699076
gi|504
gi|157164277
gi|157165403
gi|225632
gi|157165527
gi|32267367
gi|28603766
gi|157165093
gi|15020816
gi|157164830
gi|157165286
gi|157164930
gi|154175135
gi|157347169
gi|149716548
gi|157165029
gi|160893406
gi|154175276
Page 2 of 20
outer membrane protein [Campylobacter concisus 13826]
aspartyl-tRNA synthetase [Campylobacter concisus 13826]
GTP-binding protein TypA/BipA [Campylobacter curvus 525.92]
PREDICTED: similar to carboxylesterase D1 isoform 1 [Equus caballus]
casein alphaS1
heat shock protein 90 [Campylobacter curvus 525.92]
unnamed protein product [Vitis vinifera]
hypothetical protein CLOL250_00955 [Clostridium sp. L2-50]
malic enzyme NAD binding subunit [Campylobacter curvus 525.92]
179
5
2.79 %
319
5
1.57 %
Format As
Standard scoring
Show pop-ups
1.
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MudPIT scoring
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gi|76363596
Mass: 68494
RecName: Full=Serum albumin;
Query
Observed
Mr(expt)
28
366.2365
730.4584
36
379.7151
757.4156
43
386.7229
771.4312
46
395.2393
788.4640
74
411.7032
821.3918
105
422.7476
843.4806
110
424.2552
846.4958
138
433.7161
865.4176
165
449.7443
897.4740
184
457.2424
912.4702
227
474.2707
946.5268
241
477.7423
953.4700
245
479.2445
956.4744
246
479.7817
957.5488
259
486.7894
971.5642
308
501.7950 1001.5754
407
543.8295 1085.6444
449
556.2291 1110.4436
467
563.2366 1124.4586
488
570.2446 1138.4746
501
575.3112 1148.6078
503
576.2552 1150.4958
550
597.3046 1192.5946
606
616.8096 1231.6046
610
617.8143 1233.6140
626
623.8178 1245.6210
640
627.3478 1252.6810
676
426.5747 1276.7023
696
650.3561 1298.6976
Sort unassigned
Decreasing Score
Show sub-sets 0
Require bold red
Score: 1537
Queries matched: 66
emPAI: 1.94
Flags: Precursor
Expect Rank Peptide
Mr(calc)
ppm Miss Score
730.4589 -0.57
0
30
0.87
5
R.ILLSSAK.E
757.4156
0.02
0
(39)
0.05
1
K.AGCLIPK.L
771.4313 -0.03
0
48
0.0024
1
K.AGCLIPK.L
788.4644 -0.41
0
31
0.51
1
K.IVTDLTK.V
821.3919 -0.09
0
34
0.4
4
R.FNDLGEK.H
843.4814 -0.87
1
51
0.031
1
K.LDALKER.I
846.4963 -0.56
1
32
0.33
1
R.LSQKFPK.A 109
865.4181 -0.57
0
55
0.0043
1
K.ADFAEVSK.I 137 139
897.4742 -0.18
0
37
0.14
1
R.LCVLHEK.T
912.4705 -0.27
0
33
0.35
1
K.YLYEVAR.R
946.5270 -0.14
0
38
0.23
2
K.LCTVATLR.A
953.4706 -0.53
0
29
0.74
1
K.TYEATLEK.C
956.4749 -0.50
0
25
1.9
1
K.SHCIAEVK.E
957.5495 -0.65
0
56
0.0034
1
K.QSALAELVK.H
971.5651 -0.90
0
42
0.075
1
K.LVASSQLALA.- 260
1001.5757 -0.25
1
26
2.1
1
K.TPVSEKITK.C
1085.6444
0.02
1
32
0.43
1
K.KQSALAELVK.H
1110.4434
0.23
0
49
0.0033
1
K.CCTDSLAER.R
1124.4590 -0.34
0
(33)
0.15
1
K.CCTDSLAER.R
1138.4747 -0.02
0
(48)
0.0058
1
K.CCTDSLAER.R
1148.6077
0.11
0
44
0.057
1
K.LVNEVTEFAK.K
1150.4964 -0.52
0
52
0.003
1
K.EACFAEEGPK.L
1192.5949 -0.18
1
35
0.43
1
R.DTHKSEIAHR.F 548
1231.6053 -0.53
0
(21)
9.5
1
K.ACCDKPLLQK.S
1233.6142 -0.13
1
35
0.52
1
R.FNDLGEKHFK.G 609
1245.6209
0.09
0
29
1.6
1
K.ACCDKPLLQK.S
1252.6816 -0.42
0
62
0.0005
1
K.TVLGNFSAFVAK.C 641
1276.7027 -0.32
1
40
0.082
1
K.LVNEVTEFAKK.C 677
1298.6983 -0.48
0
26
2.7
1
R.HPDYSVSLLLR.I
12/9/2009
772
784
825
840
850
854
901
930
980
1000
1001
1012
1291
1300
1446
1480
1513
1514
692.2614
699.2686
718.8275
725.8350
485.9403
728.7911
756.3089
513.9443
798.4594
804.4202
805.3985
541.2874
657.9344
662.6057
778.4055
630.3124
1010.4480
1015.1220
1382.5082
1396.5226
1435.6404
1449.6554
1454.7991
1455.5676
1510.6032
1538.8111
1594.9042
1606.8258
1608.7824
1620.8404
1970.7814
1984.7953
2332.1947
2517.2205
3028.3222
3042.3442
1382.5078
1396.5235
1435.6402
1449.6558
1454.7994
1455.5646
1510.6028
1538.8127
1594.9043
1606.8249
1608.7824
1620.8406
1970.7808
1984.7965
2332.1957
2517.2209
3028.3293
3042.3449
0.32
-0.58
0.20
-0.25
-0.21
2.08
0.32
-1.03
-0.00
0.58
0.02
-0.12
0.28
-0.60
-0.44
-0.18
-2.35
-0.25
0
0
0
0
1
0
1
1
1
0
0
0
1
1
1
1
0
0
58 3.1e-005
(43)
0.0013
(49)
0.01
53
0.0034
60
0.00061
38
0.015
58
0.00022
27
2.1
67 5.6e-005
(41)
0.14
49
0.014
41
0.074
41
0.0095
(31)
0.098
21
7.4
20
10
(24)
1.7
30
0.44
Page 3 of 20
1
1
1
1
1
1
1
2
1
1
1
1
1
1
1
1
1
1
K.CAADESAENCDK.S
K.CAADESAENCDK.S
K.YICEHQDSISGK.L 824
K.YICEHQDSISGK.L
R.RHPDYSVSLLLR.I 844 845 847 849 851
K.ADFTECCPADDK.A
K.KCAADESAENCDK.S
R.LCVLHEKTPVSEK.I
K.KAPQVSTPTLVEIGR.T 978 979 981
R.LPCSENHLALALNR.L
K.DVFLGTFLYEYSR.R
R.LPCSENHLALALNR.L
K.CCGAEDKEACFAEEGPK.L 1292
K.CCGAEDKEACFAEEGPK.L 1299
K.LPESERLPCSENHLALALNR.L
K.LKPEPDAQCAAFQEDPDKFLGK.Y
K.CCAEADPPACYATVFDQFTPLVEEPK.S
K.CCAEADPPACYATVFDQFTPLVEEPK.S
2.
gi|126723507
Mass: 68554
Score: 1533
Queries matched: 65
emPAI: 1.67
preproalbumin [Equus caballus]
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
28
366.2365
730.4584
730.4589 -0.57
0
30
0.87
5
R.ILLSSAK.E
46
395.2393
788.4640
788.4644 -0.41
0
31
0.51
1
K.IVTDLTK.V
74
411.7032
821.3918
821.3919 -0.09
0
34
0.4
4
R.FNDLGEK.H
105
422.7476
843.4806
843.4814 -0.87
1
51
0.031
1
K.LDALKER.I
110
424.2552
846.4958
846.4963 -0.56
1
32
0.33
1
R.LSQKFPK.A 109
138
433.7161
865.4176
865.4181 -0.57
0
55
0.0043
1
K.ADFAEVSK.I 137 139
165
449.7443
897.4740
897.4742 -0.18
0
37
0.14
1
R.LCVLHEK.T
184
457.2424
912.4702
912.4705 -0.27
0
33
0.35
1
K.YLYEVAR.R
227
474.2707
946.5268
946.5270 -0.14
0
38
0.23
2
K.LCTVATLR.A
241
477.7423
953.4700
953.4706 -0.53
0
29
0.74
1
K.TYEATLEK.C
245
479.2445
956.4744
956.4749 -0.50
0
25
1.9
1
K.SHCIAEVK.E
246
479.7817
957.5488
957.5495 -0.65
0
56
0.0034
1
K.QSALAELVK.H
259
486.7894
971.5642
971.5651 -0.90
0
42
0.075
1
K.LVASSQLALA.- 260
308
501.7950 1001.5754 1001.5757 -0.25
1
26
2.1
1
K.TPVSEKITK.C
407
543.8295 1085.6444 1085.6444
0.02
1
32
0.43
1
K.KQSALAELVK.H
449
556.2291 1110.4436 1110.4434
0.23
0
49
0.0033
1
K.CCTDSLAER.R
467
563.2366 1124.4586 1124.4590 -0.34
0
(33)
0.15
1
K.CCTDSLAER.R
488
570.2446 1138.4746 1138.4747 -0.02
0
(48)
0.0058
1
K.CCTDSLAER.R
501
575.3112 1148.6078 1148.6077
0.11
0
44
0.057
1
K.LVNEVTEFAK.K
503
576.2552 1150.4958 1150.4964 -0.52
0
52
0.003
1
K.EACFAEEGPK.L
550
597.3046 1192.5946 1192.5949 -0.18
1
35
0.43
1
R.DTHKSEIAHR.F 548
603
616.2615 1230.5084 1230.5088 -0.26
0
(37)
0.044
1
K.CSSFQNFGER.A
606
616.8096 1231.6046 1231.6053 -0.53
0
(21)
9.5
1
K.ACCDKPLLQK.S
610
617.8143 1233.6140 1233.6142 -0.13
1
35
0.52
1
R.FNDLGEKHFK.G 609
621
623.2693 1244.5240 1244.5244 -0.29
0
44
0.014
1
K.CSSFQNFGER.A
626
623.8178 1245.6210 1245.6209
0.09
0
29
1.6
1
K.ACCDKPLLQK.S
640
627.3478 1252.6810 1252.6816 -0.42
0
62
0.0005
1
K.TVLGNFSAFVAK.C 641
676
426.5747 1276.7023 1276.7027 -0.32
1
40
0.082
1
K.LVNEVTEFAKK.C 677
696
650.3561 1298.6976 1298.6983 -0.48
0
26
2.7
1
R.HPDYSVSLLLR.I
772
692.2614 1382.5082 1382.5078
0.32
0
58 3.1e-005
1
K.CAADESAENCDK.S
784
699.2686 1396.5226 1396.5235 -0.58
0
(43)
0.0013
1
K.CAADESAENCDK.S
825
718.8275 1435.6404 1435.6402
0.20
0
(49)
0.01
1
K.YICEHQDSISGK.L 824
840
725.8350 1449.6554 1449.6558 -0.25
0
53
0.0034
1
K.YICEHQDSISGK.L
850
485.9403 1454.7991 1454.7994 -0.21
1
60
0.00061
1
R.RHPDYSVSLLLR.I 844 845 847 849 851
854
728.7911 1455.5676 1455.5646
2.08
0
38
0.015
1
K.ADFTECCPADDK.L
901
756.3089 1510.6032 1510.6028
0.32
1
58
0.00022
1
K.KCAADESAENCDK.S
930
513.9443 1538.8111 1538.8127 -1.03
1
27
2.1
2
R.LCVLHEKTPVSEK.I
980
798.4594 1594.9042 1594.9043 -0.00
1
67 5.6e-005
1
K.KAPQVSTPTLVEIGR.T 978 979 981
1000
804.4202 1606.8258 1606.8249
0.58
0
(41)
0.14
1
R.LPCSENHLALALNR.L
1001
805.3985 1608.7824 1608.7824
0.02
0
49
0.014
1
K.DVFLGTFLYEYSR.R
1012
541.2874 1620.8404 1620.8406 -0.12
0
41
0.074
1
R.LPCSENHLALALNR.L
1231
627.6448 1879.9126 1879.9138 -0.66
0
(39)
0.18
1
R.RPCFSALELDEGYVPK.E 1233 1234
1241
632.3167 1893.9283 1893.9295 -0.63
0
48
0.023
1
R.RPCFSALELDEGYVPK.E
1446
778.4055 2332.1947 2332.1957 -0.44
1
21
7.4
1
K.LPESERLPCSENHLALALNR.L
1480
630.3124 2517.2205 2517.2209 -0.18
1
20
10
1
K.LKPEPDAQCAAFQEDPDKFLGK.Y
1514 1015.1220 3042.3442 3042.3562 -3.94
1
25
1.3
3
K.CCAEADPPACYRTVFDQFTPLVEEPK.S
3.
gi|157164553
Mass: 66898
Score: 1066
Queries matched: 35
emPAI: 0.96
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
78
413.2122
824.4098
824.4102 -0.41
0
22
4
1
K.LAEAMYK.K
175
452.2392
902.4638
902.4644 -0.58
0
36
0.41
2
K.GDIMALQR.L
178
453.7557
905.4968
905.4970 -0.21
1
24
8
3
K.AFGKELNK.S
192
460.2369
918.4592
918.4593 -0.05
0
(33)
1.1
8
K.GDIMALQR.L
301
499.7744
997.5342
997.5345 -0.27
0
31
0.85
1
R.LPYHVVDR.N
343
511.7538 1021.4930 1021.4936 -0.55
0
27
2
1
R.IMGLMSNEK.N 342
355
517.7683 1033.5220 1033.5226 -0.58
0
37
0.39
1
K.EVVMVGGSTR.V 354
369
527.7490 1053.4834 1053.4834
0.00
0
(21)
6.6
3
R.IMGLMSNEK.N
410
545.2713 1088.5280 1088.5284 -0.35
0
52
0.009
1
R.NGACAVEIAGK.V 411
432
552.2790 1102.5434 1102.5441 -0.56
0
(40)
0.11
1
R.NGACAVEIAGK.V
483
568.3032 1134.5918 1134.5921 -0.20
0
23
6
1
K.VYTPQEISAK.I
12/9/2009
530
580
629
687
742
874
885
939
1024
1044
1163
1194
1277
1424
1462
1490
584.8500
607.3484
624.2852
643.8691
677.3413
739.3879
747.3851
778.4150
820.4162
830.4512
894.4979
919.9818
976.0461
1140.5550
1216.6140
854.1102
1167.6854
1212.6822
1246.5558
1285.7236
1352.6680
1476.7612
1492.7556
1554.8154
1638.8178
1658.8878
1786.9812
1837.9490
1950.0776
2279.0954
2431.2134
2559.3088
1167.6863
1212.6826
1246.5565
1285.7241
1352.6684
1476.7606
1492.7556
1554.8154
1638.8175
1658.8879
1786.9828
1837.9495
1950.0786
2279.0951
2431.2118
2559.3068
4.
gi|157165471
Mass: 95490
Score:
radical SAM domain-containing protein
Query Observed
Mr(expt)
Mr(calc)
357 517.8032 1033.5918 1033.5920
409 544.7479 1087.4812 1087.4822
441 554.3057 1106.5968 1106.5972
444 555.2903 1108.5660 1108.5665
587 608.3381 1214.6616 1214.6619
654 633.3190 1264.6234 1264.6234
665 635.3495 1268.6844 1268.6837
778 694.3701 1386.7256 1386.7256
787 700.3536 1398.6926 1398.6932
899 754.9510 1507.8874 1507.8875
919 765.4120 1528.8094 1528.8097
975 531.6337 1591.8793 1591.8794
997 801.4196 1600.8246 1600.8250
1032 822.4235 1642.8324 1642.8315
1155 888.9382 1775.8618 1775.8618
1178 901.9063 1801.7980 1801.7981
5.
gi|7331218
Mass: 65978
Query Observed
Mr(expt)
37 379.7295
757.4444
88 416.7477
831.4808
146 437.7530
873.4914
261 487.2691
972.5236
303 500.2256
998.4366
327 508.2235 1014.4324
352 517.2614 1032.5082
468 563.2741 1124.5336
536 590.3036 1178.5926
655 633.3222 1264.6298
702 651.8611 1301.7076
725 670.8378 1339.6610
773 692.3496 1382.6846
782 697.3699 1392.7252
869 738.3962 1474.7778
912 762.3967 1522.7788
1113 858.9291 1715.8436
1142 883.3716 1764.7286
-0.74
-0.30
-0.51
-0.38
-0.28
0.42
0.06
0.02
0.23
-0.03
-0.90
-0.27
-0.48
0.16
0.67
0.78
1
0
1
1
0
1
1
0
0
0
1
1
0
0
0
1
48
0.0048
45
0.027
34
0.19
71 6.7e-005
58
0.0018
63
0.0006
(20)
16
76 2.5e-005
38
0.17
34
0.4
63
0.00043
47
0.019
39
0.043
96 2.9e-007
56
0.0026
45
0.034
Page 4 of 20
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
R.VPLVQEEVKK.A
K.EAGTIAGLNVLR.I
K.KDDDVIDAEVE.K.INEVIKDAGLSK.S
R.NQADALVHQTEK.S
K.SDIKEVVMVGGSTR.V 872
K.SDIKEVVMVGGSTR.V
K.SLGNFNLEGIPAAPR.G
K.FEGMIDSLVGETITK.I
R.IINEPTAAALAYGLDK.K
R.IINEPTAAALAYGLDKK.E
R.AKFEGMIDSLVGETITK.I
K.SVNPDEVVAIGAAIQGAVIK.G 1278
K.AQNITISGSSGLSEDEINSMVK.D
K.ELSSAQETEINLPFITADATGPK.H 1461 1463
K.KELSSAQETEINLPFITADATGPK.H 1489
797
Queries matched: 20
emPAI: 0.50
ppm Miss Score
Expect Rank Peptide
-0.16
0
42
0.05
1
R.IADALLHPGK.V
-0.87
0
34
0.14
1
R.SETAQYFDK.A
-0.29
0
61
0.0008
1
R.GEVLAGAYVTK.N
-0.43
0
62
0.00099
1
K.ATNYAFPVAR.I
-0.22
0
67
0.00025
1
K.GAVIGGVNSASVGK.F
0.03
0
52
0.0089
1
K.TGDLVCVFNAR.G
0.60
0
48
0.013
1
R.LHSQLSQTSLR.E
0.07
0
80 1.3e-005
1
K.AAAVAGTGQSWLQK.A
-0.40
0
41
0.081
1
K.GLQSVFAGSYGWK.S
-0.02
0
77 3.1e-006
1
K.VVVIWGANPLATLR.I 898
-0.15
0
50
0.012
1
K.NGSANVLTIDIPTSK.L
-0.09
0
33
0.21
1
K.LANGNISHTALVNIR.K 974
-0.19
0
73 5.1e-005
1
R.TVVVHEAYWTPTAK.M 998
0.61
0
28
1.8
1
K.SYLQNPDSPKPELR.G 1031
0.05
0
29
1.5
1
R.GIDEWVEVPYEEAIK.L
-0.04
0
20
5
1
K.LAEGAWYDGFDSGICK.N
Score: 775
Mr(calc)
757.4446
831.4814
873.4920
972.5240
998.4379
1014.4328
1032.5087
1124.5349
1178.5931
1264.6299
1301.7078
1339.6619
1382.6830
1392.7249
1474.7780
1522.7813
1715.8438
1764.7275
Queries matched: 22
emPAI: 0.55
ppm Miss Score
Expect Rank
-0.24
1
24
15
1
-0.66
0
29
1.3
2
-0.60
0
28
3.8
1
-0.33
0
46
0.047
1
-1.25
0
38
0.064
1
-0.36
0
(29)
0.27
1
-0.46
0
30
1
1
-1.14
0
21
5.5
1
-0.40
0
55
0.004
1
-0.05
0
55
0.005
1
-0.14
0
75 4.1e-005
1
-0.64
1
54
0.0036
1
1.18
0
45
0.031
1
0.27
1
56
0.0024
1
-0.09
0
58
0.0018
1
-1.63
1
32
0.88
1
-0.09
0
72 6.7e-005
1
0.65
0
57
0.00063
1
Peptide
R.RVDQLK.S
K.SISISVAR.G
R.SLVNLGGSK.S
K.IEISELNR.V
K.DVDGAYMTK.V
K.DVDGAYMTK.V
R.TLLEGEESR.M
K.AEAESLYQSK.Y
K.YEELQITAGR.H 535
R.TNAENEFVTIK.K
R.SLDLDSIIAEVK.A
K.SKAEAESLYQSK.Y
K.SLNNQFASFIDK.V
R.TNAENEFVTIKK.D 781
R.FLEQQNQVLQTK.W
R.LLRDYQELMNTK.L
K.QISNLQQSISDAEQR.G 1112
R.FSSCGGGGGSFGAGGGFGSR.S 1141
gi|11935049
Mass: 66027
Score: 775
Queries matched: 22
gi|119395750
Mass: 65999
Score: 775
Queries matched: 22
gi|160961491
Mass: 65450
Score: 775
Queries matched: 22
6.
gi|28317
Mass: 59492
Score: 714
Query Observed
Mr(expt)
Mr(calc)
64 404.2031
806.3916
806.3923
294 497.2527
992.4908
992.4927
351 516.3026 1030.5906 1030.5910
386 532.8084 1063.6022 1063.6026
414 545.7687 1089.5228 1089.5237
435 553.7667 1105.5188 1105.5186
521 583.2958 1164.5770 1164.5775
559 601.3118 1200.6090 1200.6098
650 631.8021 1261.5896 1261.5899
749 453.2441 1356.7105 1356.7110
758 683.3231 1364.6316 1364.6320
779 695.8443 1389.6740 1389.6736
Queries matched: 18
emPAI: 0.62
ppm Miss Score
Expect Rank
-0.76
0
25
3.6
1
-1.84
0
37
0.24
1
-0.36
0
71 4.2e-005
1
-0.30
1
42
0.04
1
-0.77
0
38
0.17
1
0.22
0
(33)
0.61
1
-0.36
0
50
0.012
1
-0.67
0
30
0.84
1
-0.17
0
53
0.0044
1
-0.35
1
23
6.6
1
-0.27
0
42
0.055
1
0.36
0
61
0.00086
1
Peptide
R.LAADDFR.L
K.YENEVALR.Q
R.VLDELTLTK.A
R.LASYLDKVR.A
K.VTMQNLNDR.L
K.VTMQNLNDR.L
R.LENEIQTYR.S
R.QSVEADINGLR.R
R.SLLEGEGSSGGGGR.G
R.QSVEADINGLRR.V
R.SQYEQLAEQNR.K
K.QSLEASLAETEGR.Y
12/9/2009
822
883
1098
1306
1508
1510
717.8887
747.3702
854.3897
998.9893
963.4684
968.7977
1433.7628
1492.7258
1706.7648
1995.9640
2887.3834
2903.3713
1433.7626
1492.7270
1706.7649
1995.9636
2887.3804
2903.3753
0.15
-0.75
-0.04
0.21
1.03
-1.39
1
1
0
0
0
0
30
0.92
37
0.23
78 8.9e-006
55
0.0036
(28)
1.4
41
0.076
1
1
1
1
1
1
Page 5 of 20
K.IRLENEIQTYR.S
R.SQYEQLAEQNRK.D
K.ELTTEIDNNIEQISSYK.S
Mass: 58792
Score: 714
Queries matched: 18
gi|21961605
gi|114667509
Mass: 62761
Score: 714
Queries matched: 18
gi|114667511
Mass: 58244
Score: 714
Queries matched: 18
Mass: 56692
Score: 714
Queries matched: 18
gi|114667513
gi|119581085
Mass: 63308
Score: 714
Queries matched: 18
gi|195972866
Mass: 58766
Score: 714
Queries matched: 18
gi|251757513
Mass: 58818
Score: 714
Queries matched: 18
gi|114667507
Mass: 100282
Score: 711
Queries matched: 18
7.
gi|154175431
Mass: 67179
Score: 676
Queries matched: 26
emPAI: 0.54
molecular chaperone DnaK [Campylobacter curvus 525.92]
Query
Observed
Mr(expt)
Mr(calc)
78
413.2122
824.4098
824.4102 -0.41
0
22
4
1
K.LAEAMYK.K
178
453.7557
905.4968
905.4970 -0.21
1
24
8
3
K.AFGKELNK.S
301
499.7744
997.5342
997.5345 -0.27
0
31
0.85
1
R.LPYHVVDR.N
343
511.7538 1021.4930 1021.4936 -0.55
0
27
2
1
R.IMGLMSNEK.N 342
355
517.7683 1033.5220 1033.5226 -0.58
0
37
0.39
1
K.EVVMVGGSTR.V 354
369
527.7490 1053.4834 1053.4834
0.00
0
(21)
6.6
3
R.IMGLMSNEK.N
410
545.2713 1088.5280 1088.5284 -0.35
0
52
0.009
1
R.NGACAVEIAGK.V 411
432
552.2790 1102.5434 1102.5441 -0.56
0
(40)
0.11
1
R.NGACAVEIAGK.V
483
568.3032 1134.5918 1134.5921 -0.20
0
23
6
1
K.VYTPQEISAK.I
530
584.8500 1167.6854 1167.6863 -0.74
1
48
0.0048
1
R.VPLVQEEVKK.A
580
607.3484 1212.6822 1212.6826 -0.30
0
45
0.027
1
K.EAGTIAGLNVLR.I
629
624.2852 1246.5558 1246.5565 -0.51
1
34
0.19
1
K.KDDDVIDAEVE.687
643.8691 1285.7236 1285.7241 -0.38
1
51
0.0063
2
K.INEVVKEAGLSK.S
760
683.3486 1364.6826 1364.6823
0.25
1
45
0.044
1
K.LKEDAEAYLGEK.V
1044
830.4512 1658.8878 1658.8879 -0.03
0
34
0.4
1
R.IINEPTAAALAYGLDK.K
1163
894.4979 1786.9812 1786.9828 -0.90
1
63 0.00043
1
R.IINEPTAAALAYGLDKK.E
1277
976.0461 1950.0776 1950.0786 -0.48
0
39
0.043
1
K.SVNPDEVVAIGAAIQGAVIK.G 1278
1462 1216.6140 2431.2134 2431.2118
0.67
0
56
0.0026
1
K.ELSSAQETEINLPFITADATGPK.H 1461 1463
1490
854.1102 2559.3088 2559.3068
0.78
1
45
0.034
1
K.KELSSAQETEINLPFITADATGPK.H 1489
8.
gi|189027839
Mass: 77053
Score: 628
Queries matched: 19
emPAI: 0.72
RecName: Full=Elongation factor G; Short=EF-G
Query Observed
Mr(expt)
Mr(calc)
35 379.2319
756.4492
756.4494 -0.20
0
33
1.3
1
K.GVVDLVR.M
76 412.7201
823.4256
823.4262 -0.66
0
26
2.8
1
K.LAASMGFK.E
77 412.7240
823.4334
823.4341 -0.75
0
46
0.004
1
R.IGANFFR.V
537 590.3276 1178.6406 1178.6407 -0.08
0
67 0.00017
1
R.NIGIAAHIDAGK.T
554 598.8101 1195.6056 1195.6060 -0.27
0
21
11
1
R.ILFFTGMSHK.I
817 716.3658 1430.7170 1430.7154
1.16
0
26
3.4
1
K.VDAEVGQPQVAYR.E
861 734.3958 1466.7770 1466.7769
0.08
0
40
0.1
1
R.GITITSAATTAFWK.G
1018 819.4396 1636.8646 1636.8647 -0.04
0
(35)
0.24
1
K.IMTDPFVGQLTFIR.V
1040 827.4356 1652.8566 1652.8596 -1.81
0
42
0.058
1
K.IMTDPFVGQLTFIR.V
1043 829.4293 1656.8440 1656.8433
0.46
0
43
0.057
1
R.MDFPEPVISVAVEPK.T
1050 835.4197 1668.8248 1668.8246
0.13
1
43
0.05
1
K.FFAGEELTEEEIKK.G
1051 837.4263 1672.8380 1672.8382 -0.10
0
(41)
0.098
1
R.MDFPEPVISVAVEPK.T
1058 840.4061 1678.7976 1678.7971
0.32
0
57
0.0025
1
K.LIEAVSETDDSLMEK.F
1153 887.9644 1773.9142 1773.9149 -0.35
0
52
0.0068
1
K.EALQSGVLAGYPVEDVK.V
1182 911.9890 1821.9634 1821.9625
0.53
0
34
0.28
1
K.ANPVPIQIPIGAEDNFK.G
1191 918.4680 1834.9214 1834.9214
0.05
1
54
0.0057
1
R.EFKVDAEVGQPQVAYR.E 1190
1202 923.9832 1845.9518 1845.9512
0.33
0
44
0.041
1
R.IEPLPAASGFEFVNDIK.G 1201
9.
gi|157165732
Mass: 104152
Score: 578
Queries matched: 16
emPAI: 0.32
nitrate reductase [Campylobacter concisus 13826]
Query
Observed
Mr(expt)
Mr(calc)
176
452.2424
902.4702
902.4709 -0.72
1
21
12
1
R.DLEDGKVK.F
224
472.7507
943.4868
943.4876 -0.77
0
26
3.4
3
R.EVGTFVHR.L
344
512.2354 1022.4562 1022.4570 -0.71
0
21
6.2
1
K.WGAYGNAER.R
489
570.3063 1138.5980 1138.5982 -0.17
0
38
0.11
1
K.VVNLSTYSTR.T
490
570.8076 1139.6006 1139.6009 -0.20
0
62 0.00062
1
R.LPADMVVGNPK.H
509
578.8048 1155.5950 1155.5958 -0.65
0
(33)
0.46
1
R.LPADMVVGNPK.H
604
616.3176 1230.6206 1230.6204
0.21
1
38
0.19
1
K.AGDTLENKNAAK.A
712
655.8531 1309.6916 1309.6918 -0.11
0
40
0.081
1
K.ALAPYTLDFTAK.V
727
671.3162 1340.6178 1340.6183 -0.35
0
45
0.03
1
K.QAFDVMEAQFR.K
783
698.3355 1394.6564 1394.6565 -0.04
0
69 0.00016
1
K.YAPSELDTAATEK.S
12/9/2009
911
954
965
1295
1307
1379
762.3826
788.9360
792.9224
991.4815
999.4794
1074.5270
1522.7506
1575.8574
1583.8302
1980.9484
1996.9442
2147.0394
1522.7515
1575.8582
1583.8308
1980.9503
1996.9452
2147.0430
-0.54
-0.47
-0.33
-0.92
-0.47
-1.63
1
0
0
0
0
0
26
4
25
2.1
30
0.98
61 0.00094
(57) 0.0019
76
3e-005
1
1
1
1
1
1
Page 6 of 20
K.KYAPSELDTAATEK.S
K.VADLILPTAMIYEK.W
K.LPAGTLSGTPGSHYVK.M
R.EMDNFIVVSDPYPGISAK.V
R.EMDNFIVVSDPYPGISAK.V
K.QALPGSGAFSLTGQPSACGTAR.E
10.
gi|194676990
Mass: 45494
Score:
Query Observed
Mr(expt)
Mr(calc)
14 357.6982
713.3818
713.3820
51 396.2422
790.4698
790.4701
151 439.7526
877.4906
877.4909
201 465.7662
929.5178
929.5182
285 493.7974
985.5802
985.5808
297 497.8056
993.5966
993.5971
347 513.8006 1025.5866 1025.5869
399 540.2956 1078.5766 1078.5771
456 557.8448 1113.6750 1113.6757
586 608.3036 1214.5926 1214.5931
1146 884.4131 1766.8116 1766.8112
1169 896.4262 1790.8378 1790.8363
1470 831.3978 2491.1716 2491.1754
551
Queries matched: 17
emPAI: 0.52
taurus]
-0.24
0
30
0.63
1
R.NSLQPR.H 15
-0.33
1
22
9
1
K.LLDKFR.K
-0.28
0
33
0.53
1
K.LINEYVK.N
-0.37
0
41
0.15
1
K.LTPETLTR.W
-0.54
0
52 0.0064
1
R.DILSQLGIK.K
-0.46
0
50 0.0026
1
K.LAVSQVIHK.A
-0.26
1
37
0.11
1
K.QLALKDPNK.N
-0.41
0
57 0.0021
1
R.HIDELSLPR.F 400
-0.62
1
35
0.14
1
R.DILSQLGIKK.I
-0.41
0
52 0.0055
1
R.FSISSDYQLR.D 584
0.28
0
75 3e-005
1
K.IFTSDADFSGITNDHK.L 1145
0.88
0
29
1.2
1
-1.55
1
38
0.15
1
11.
gi|114667176
Mass: 107386
Score:
Query Observed
Mr(expt)
Mr(calc)
64 404.2031
806.3916
806.3923
183 457.2078
912.4010
912.4011
380 530.7852 1059.5558 1059.5560
386 532.8084 1063.6022 1063.6026
387 533.2529 1064.4912 1064.4920
414 545.7687 1089.5228 1089.5237
435 553.7667 1105.5188 1105.5186
510 579.2988 1156.5830 1156.5836
605 616.8026 1231.5906 1231.5906
613 618.2680 1234.5214 1234.5215
1168 896.3677 1790.7208 1790.7205
1473 837.3820 2509.1242 2509.1245
506
Queries matched: 13
emPAI: 0.16
-0.76
0
25
3.6
1
R.LAADDFR.T
-0.08
0
30
0.55
1
R.MTLDDFR.I 182
-0.17
0
34
0.61
1
K.TLLDIDNTR.M
-0.30
1
42
0.04
1
R.LASYLDKVR.A
-0.74
0
32
0.56
1
K.STMQELNSR.L
-0.77
0
38
0.17
1
K.VTMQNLNDR.L
0.22
0
(33)
0.61
1
K.VTMQNLNDR.L
-0.52
0
58
0.0015
1
R.QGVDADINGLR.Q
0.07
0
52
0.0073
1
-0.00
0
64 0.00013
1
R.FSSSSGYGGGSSR.V
0.20
0
78
2e-006
1
-0.12
0
53
0.003
1
12.
gi|38683423
Mass: 46972
Query Observed
Mr(expt)
14 357.6982
713.3818
51 396.2422
790.4698
151 439.7526
877.4906
201 465.7662
929.5178
285 493.7974
985.5802
309 502.3052 1002.5958
347 513.8006 1025.5866
456 557.8448 1113.6750
484 568.3214 1134.6282
576 605.3164 1208.6182
1147 884.9022 1767.7898
1169 896.4262 1790.8378
1470 831.3978 2491.1716
13.
14.
gi|62126072
Mass: 46687
Query Observed
Mr(expt)
14 357.6982
713.3818
51 396.2422
790.4698
108 424.2298
846.4450
151 439.7526
877.4906
201 465.7662
929.5178
297 497.8056
993.5966
347 513.8006 1025.5866
396 358.8768 1073.6086
399 540.2956 1078.5766
484 568.3214 1134.6282
586 608.3036 1214.5926
1146 884.4131 1766.8116
Score: 503
Mr(calc)
713.3820
790.4701
877.4909
929.5182
985.5808
1002.5974
1025.5869
1113.6757
1134.6285
1208.6190
1767.7952
1790.8363
2491.1754
emPAI: 0.23
-0.24
0
30
0.63
1
-0.33
1
22
9
1
-0.28
0
33
0.53
1
-0.37
0
41
0.15
1
-0.54
0
52 0.0064
1
-1.59
0
30
0.3
1
-0.26
1
37
0.11
1
-0.62
1
35
0.14
1
-0.19
0
46
0.021
1
-0.59
0
36
0.39
1
-3.02
0
73 3e-005
1
0.88
0
29
1.2
1
-1.55
1
38
0.15
1
Score: 501
Mr(calc)
713.3820
790.4701
846.4456
877.4909
929.5182
993.5971
1025.5869
1073.6089
1078.5771
1134.6285
1214.5931
1766.8112
Queries matched: 15
Queries matched: 16
Peptide
R.NSLQPR.L 15
K.LLDKFR.K
K.LINEYVK.N
K.LTPETLTR.W
K.DILSQLGIK.K
K.LAVSHVIHK.A
K.QLALKDPNK.N
K.DILSQLGIKK.I
K.ETQSIIFLGK.V
R.FSISSHYQLK.D
K.IFTSDADFSGITDDHK.L 1148
emPAI: 0.31
-0.24
0
30
0.63
1
-0.33
1
22
9
1
-0.60
0
33
0.87
1
-0.28
0
33
0.53
1
-0.37
0
41
0.15
1
-0.46
0
50 0.0026
1
-0.26
1
37
0.11
1
-0.34
1
26
2.4
1
-0.41
0
57 0.0021
1
-0.19
0
46
0.021
1
-0.41
0
52 0.0055
1
0.28
0
75 3e-005
1
Peptide
R.NSLQPR.H 15
K.LLDKFR.K
K.VPMMTLR.L
K.LINEYVK.N
K.LTPETLTR.W
K.LAVSQVIHK.A
K.QLALKDPNK.N
R.VKVPMMTLR.L
R.HIDELSLPR.F 400
K.ETQSIIFLGK.V
R.FSISSDYQLR.D 584
K.IFTSDADFSGITNDHK.L 1145
gi|157164629
Mass: 43586
Score: 482
Queries matched: 17
emPAI: 1.07
Expect Rank Peptide
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
141
434.2557
866.4968
866.4974 -0.61
0
30
0.66
1
R.EHILLSR.Q
362
524.7380 1047.4614 1047.4621 -0.65
0
52
0.0041
1
K.AGQDGEWSAK.I
704
652.3824 1302.7502 1302.7507 -0.35
0
69
4e-005
1
K.TTLTAAISAVLSR.K
1087
848.9775 1695.9404 1695.9407 -0.13
0
52
0.0036
1
1095
851.9067 1701.7988 1701.7992 -0.20
0
79 1.2e-005
1
12/9/2009
1115
1203
1287
1296
1354
1363
1451
1483
1487
859.9046
616.6544
983.4852
991.4835
703.0298
708.3600
1190.0890
1260.6020
846.0678
1717.7946
1846.9414
1964.9558
1980.9524
2106.0676
2122.0582
2378.1634
2519.1894
2535.1816
1717.7941
1846.9425
1964.9587
1980.9536
2106.0667
2122.0616
2378.1642
2519.1884
2535.1833
15.
gi|194038347
Mass: 46611
Score:
PREDICTED: alpha-1-antichymotrypsin 3
Query Observed
Mr(expt)
Mr(calc)
20 360.1913
718.3680
718.3683
89 417.2220
832.4294
832.4299
113 425.2197
848.4248
848.4248
249 480.7791
959.5436
959.5440
284 493.7793
985.5440
985.5444
375 353.1893 1056.5461 1056.5465
458 558.8296 1115.6446 1115.6451
667 635.8668 1269.7190 1269.7180
820 717.3726 1432.7306 1432.7310
991 800.9143 1599.8140 1599.8144
1071 845.4078 1688.8010 1688.8006
1389 725.3738 2173.0996 2173.1015
1440 773.7264 2318.1574 2318.1583
16.
gi|121531628
Mass: 46282
Query
Observed
Mr(expt)
14
357.6982
713.3818
44
389.7207
777.4268
237
476.2608
950.5070
248
480.7627
959.5108
323
506.7948 1011.5750
347
513.8006 1025.5866
464
561.3135 1120.6124
591
407.2274 1218.6604
667
635.8668 1269.7190
733
673.8692 1345.7238
1340 1037.0000 2071.9854
1371
714.6882 2141.0428
0.31
-0.59
-1.46
-0.60
0.42
-1.62
-0.30
0.42
-0.68
(34)
25
(21)
50
(32)
35
52
39
(22)
0.32
4.5
9.2
0.012
0.65
0.34
0.0073
0.13
5.6
1
1
1
1
1
1
1
1
1
R.GITIATSHIEYETEKR.H
R.ATDKDLLMPIEDVFSISGR.G 1364
478
Queries matched: 18
emPAI: 0.41
[Sus scrofa]
-0.40
0
31
1.2
1
R.MAAVEAK.L
-0.54
0
28
2.5
1
K.VPMMSLR.G
0.03
0
(21)
8.8
1
K.VPMMSLR.G
-0.36
0
41
0.13
1
R.IDALYLPR.F
-0.36
0
47
0.022
1
K.QLVSLDPSK.N
-0.37
1
31
0.92
1
R.WRDSLQPR.R 374
-0.40
1
27
1.5
1
R.RIDALYLPR.F
0.82
0
38
0.11
1
R.GPTLTELLEGLK.F
-0.26
1
27
2.3
1
K.LLDEFRQDAQAK.F
-0.24
1
70 0.00011
1
R.FSVSGDYQLKDTLK.Q
0.27
0
66 0.00026
1
-0.90
1
51
0.0091
1
K.VFTHEADLSGVTGDNKLGVSK.V 1388 1391
-0.40
0
21
10
1
R.TPVDPHAIVPSNTDFAFSLYK.Q
Score: 474
Mr(calc)
713.3820
777.4272
950.5073
959.5110
1011.5753
1025.5869
1120.6128
1218.6608
1269.7180
1345.7241
2071.9851
2141.0429
0
1
0
0
1
1
0
0
0
Page 7 of 20
Queries matched: 20
emPAI: 0.23
-0.24
0
30
0.63
1
-0.49
0
26
2.1
1
-0.24
0
45
0.046
1
-0.14
0
31
0.97
1
-0.24
0
26
2.2
1
-0.26
1
37
0.11
1
-0.33
0
64 0.00039
1
-0.36
1
35
0.38
1
0.82
0
38
0.11
1
-0.21
1
61 0.00087
1
0.17
0
37
0.22
1
-0.06
1
44
0.046
1
Peptide
R.NSLQPR.R 15
K.IEELFK.D
K.SLINDYVK.N
K.LTPEMLTR.W
R.IHELYLPK.F
K.QLALKDPNK.N
K.DTQSIIFLGK.V
K.LLDKFIEDAR.V 589 590
R.GPTLTEILEGLK.F
K.IEELFKDLSPR.T 732 734
R.VLYSSEAFPTNFRDPEAAK.S 1372
17.
gi|255322039
Mass: 76997
Score: 461
Queries matched: 15
emPAI: 0.46
translation elongation factor G [Campylobacter showae RM3277]
Query Observed
Mr(expt)
Mr(calc)
76 412.7201
823.4256
823.4262 -0.66
0
26
2.8
1
K.LAASMGFK.E
537 590.3276 1178.6406 1178.6407 -0.08
0
67 0.00017
1
R.NIGIAAHIDAGK.T
554 598.8101 1195.6056 1195.6060 -0.27
0
21
11
1
R.ILFFTGMSHK.I
646 629.8190 1257.6234 1257.6241 -0.51
1
30
0.8
1
K.TVEQEYKYAK.Q
817 716.3658 1430.7170 1430.7154
1.16
0
26
3.4
1
K.VDAEVGQPQVAYR.E
1018 819.4396 1636.8646 1636.8647 -0.04
0
(35)
0.24
1
K.IMTDPFVGQLTFIR.V
1040 827.4356 1652.8566 1652.8596 -1.81
0
42
0.058
1
K.IMTDPFVGQLTFIR.V
1043 829.4293 1656.8440 1656.8433
0.46
0
43
0.057
1
K.MDFPEPVISVAVEPK.T
1051 837.4263 1672.8380 1672.8382 -0.10
0
(41)
0.098
1
K.MDFPEPVISVAVEPK.T
1058 840.4061 1678.7976 1678.7971
0.32
0
57
0.0025
1
K.LIEAVSETDDSLMEK.F
1153 887.9644 1773.9142 1773.9149 -0.35
0
52
0.0068
1
K.EALQSGVLAGYPVEDVK.V
1191 918.4680 1834.9214 1834.9214
0.05
1
54
0.0057
1
R.EFKVDAEVGQPQVAYR.E 1190
1202 923.9832 1845.9518 1845.9512
0.33
0
44
0.041
1
R.LEPLPAASGFEFVNDIK.G 1201
18.
gi|115495209
zymogen granule
Query Observed
30 367.2339
63 403.7112
160 443.7280
172 451.7254
515 580.7901
534 587.7976
651 632.7741
653 633.2860
660 634.2754
679 641.2834
685 642.7740
744 678.8130
1123 865.3618
1130 869.9010
1455 804.6764
1506 939.7753
Mass: 59194
Score:
Mr(expt)
Mr(calc)
732.4532
732.4534
805.4078
805.4083
885.4414
885.4419
901.4362
901.4368
1159.5656 1159.5656
1173.5806 1173.5812
1263.5336 1263.5336
1264.5574 1264.5580
1266.5362 1266.5373
1280.5522 1280.5529
1283.5334 1283.5340
1355.6114 1355.6115
1728.7090 1728.7083
1737.7874 1737.7880
2411.0074 2411.0084
2816.3041 2816.3035
19.
gi|157164057
Mass: 61832
453
Queries matched: 20
emPAI: 0.46
2 [Bos taurus]
-0.21
0
42
0.022
1
R.FNLVLK.N
-0.54
0
25
3
1
R.FVGDGGVR.M
-0.47
0
48
0.0062
1
R.FSVQMFK.F 161
-0.59
0
(33)
0.41
1
R.FSVQMFK.F 173
0.08
0
40
0.12
1
R.LEGTPQCSLR.Y
-0.46
0
(35)
0.42
1
R.LEGTPQCSLR.Y
0.06
0
26
0.71
1
R.TCRPEEECR.L
-0.46
0
(28)
1.2
1
R.MPEDCVPTFR.C
-0.84
0
(21)
3
1
R.MPEDCVPTFR.C
-0.55
0
42
0.031
1
R.MPEDCVPTFR.C
-0.40
0
35
0.073
1
R.YCTDPATAEDK.C
-0.02
0
21
5.7
1
R.LVSGTWGCFCR.Q
0.43
1
41
0.013
1
R.YCTDPATAEDKCDR.T 1122
-0.30
1
28
1.3
1
K.NCYATPTEDKTDPVK.Y
-0.43
1
39
0.03
1
R.STENTEDIQGCDSDKHGWYR.F 1456
0.20
0
67 0.00016
1
R.QSEEENWISVTNPTQAGACGNILER.N
Score: 440
Queries matched: 11
emPAI: 0.36
12/9/2009
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
27 366.2238
730.4330
730.4337 -0.96
0
21
126 430.7213
859.4280
859.4287 -0.75
0
36
134 432.7447
863.4748
863.4753 -0.50
0
31
465 562.2908 1122.5670 1122.5669
0.12
1
43
735 674.3687 1346.7228 1346.7234 -0.42
0
25
754 681.8587 1361.7028 1361.7038 -0.71
1
72
946 785.9380 1569.8614 1569.8614
0.04
0
61
1224 934.4712 1866.9278 1866.9265
0.74
0
41
1249 948.9464 1895.8782 1895.8789 -0.32
0
61
1413 747.3882 2239.1428 2239.1444 -0.73
1
24
1452 795.0604 2382.1594 2382.1591
0.13
0
26
Expect Rank
20
1
0.34
2
1.4
4
0.059
1
3.5
1
7e-005
1
0.00053
1
0.08
1
0.00071
1
4.2
1
2.9
1
Page 8 of 20
Peptide
K.VVITGSR.N
K.AGDELEVK.I
K.FSVGDIVK.G
K.VISYDNEKR.H
K.NLLPKPFDEFK.A
R.KEDLGSVDASTLK.I
K.IVSINGDEVLIDVGK.K
K.GGFITQDANGVEFFLPR.T
K.AFIEAYDPENSGEIDVK.V
K.KSEGILNVSEITDANGNLTHK.V
K.AATPDPWEEIINDGLEVGDTIK.V
20.
gi|194038353
Mass: 47192
Score: 434
Queries matched: 12
emPAI: 0.31
Query Observed
Mr(expt)
Mr(calc)
17 357.7416
713.4686
713.4687 -0.08
0
22
3.4
1
K.IVELIK.K
102 421.7890
841.5634
841.5637 -0.25
1
34
0.12
1
K.IVELIKK.L
284 493.7793
985.5440
985.5444 -0.36
0
47
0.022
1
K.QLVSLDPSK.N
375 353.1893 1056.5461 1056.5465 -0.37
1
31
0.92
1
R.WRDSLQPR.R 374
419 364.2208 1089.6406 1089.6407 -0.10
1
30
0.38
2
R.RIDALHLPR.F
464 561.3135 1120.6124 1120.6128 -0.33
0
64 0.00039
1
R.DTQSIIFLGK.V
567 602.8375 1203.6604 1203.6612 -0.59
1
53
0.0069
1
K.LLDKFVQDAR.E
794 706.8830 1411.7514 1411.7500
1.03
0
40
0.088
1
R.FDSPFLFATVLR.D
816 715.4078 1428.8010 1428.8017 -0.44
0
58
0.0014
1
R.TVAVLVNYIYFK.A
948 787.4071 1572.7996 1572.8003 -0.44
0
34
0.48
1
R.MAAVEANLLPEMLR.R
1173 599.6530 1795.9372 1795.9356
0.89
1
23
5.1
1
R.FSISSDYKLEEILPR.L
21.
gi|435476
Mass: 62092
Score: 430
cytokeratin 9 [Homo sapiens]
Query Observed
Mr(expt)
Mr(calc)
183 457.2078
912.4010
912.4011
380 530.7852 1059.5558 1059.5560
387 533.2529 1064.4912 1064.4920
510 579.2988 1156.5830 1156.5836
571 603.8052 1205.5958 1205.5962
605 616.8026 1231.5906 1231.5906
613 618.2680 1234.5214 1234.5215
1168 896.3677 1790.7208 1790.7205
1473 837.3820 2509.1242 2509.1245
Queries matched: 10
emPAI: 0.29
-0.08
0
30
0.55
1
-0.17
0
34
0.61
1
-0.74
0
32
0.56
1
-0.52
0
58
0.0015
1
-0.27
0
29
1.3
1
0.07
0
52
0.0073
1
-0.00
0
64 0.00013
1
0.20
0
78
2e-006
1
-0.12
0
53
0.003
1
Peptide
R.MTLDDFR.I 182
K.TLLDIDNTR.M
K.STMQELNSR.L
R.QGVDADINGLR.Q
R.QVLDNLTMEK.S
R.FSSSSGYGGGSSR.V
gi|55956899
Mass: 62027
Score: 430
Queries matched: 10
22.
gi|157165097
Mass: 57700
Score: 409
Queries matched: 10
emPAI: 0.47
chaperonin GroEL [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
579 606.8326 1211.6506 1211.6510 -0.28
0
55 0.0028
1
R.NVTAGANPIEVK.R
693 647.3210 1292.6274 1292.6282 -0.57
1
32
0.62
1
K.VGAATETEMKEK.K
730 672.8716 1343.7286 1343.7296 -0.74
1
39
0.091
1
K.VGKDGVITVEEAK.S
762 684.8835 1367.7524 1367.7521
0.26
1
37
0.11
1
R.NVTAGANPIEVKR.G
916 764.8881 1527.7616 1527.7609
0.47
0
39
0.11
1
R.GYLSPYFITNPEK.M
988 799.8812 1597.7478 1597.7471
0.46
1
53
0.004
1
K.AQIAETTSDYDKEK.L
1200 923.9736 1845.9326 1845.9319
0.38
0
58 0.0019
1
K.EIAQIATISANSDESIGK.L
1253 952.4940 1902.9734 1902.9720
0.74
1
66 0.0003
1
K.EVELKDTIENMGASLVR.E 1252
1368 711.3587 2131.0543 2131.0546 -0.14
0
29
1.4
1
K.TNDQAGDGTTTATVLAHAIFK.E
23.
gi|157165518
Mass: 53621
Score: 388
Queries matched: 10
emPAI: 0.35
elongation factor P (EF-P) [Campylobacter concisus 13826]
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
215
470.3152
938.6158
938.6164 -0.61
1
28
0.16
1
R.LIDKILPK.G
221
471.7901
941.5656
941.5658 -0.17
0
41
0.099
1
K.GLINIVTGR.G
421
546.8001 1091.5856 1091.5862 -0.55
1
25
3.1
2
K.FKDEAELIK.M
662
634.3904 1266.7662 1266.7659
0.24
1
24
0.93
1
R.RIIPATLELGGK.S
830
721.3517 1440.6888 1440.6885
0.25
0
53
0.006
1
K.SANIFFSDANLDK.A
907
759.3577 1516.7008 1516.7005
0.22
0
87 1.8e-006
1
K.IADATEEDVNDAVR.A
966
792.9534 1583.8922 1583.8923 -0.03
0
57
0.00094
1
R.VAQEEIFGPVGVVIK.F
1319 1014.9820 2027.9494 2027.9510 -0.76
0
44
0.04
1
1329 1022.9800 2043.9454 2043.9459 -0.23
0
(30)
0.74
1
1355 1056.5300 2111.0454 2111.0476 -1.03
0
30
1.1
1
R.VWINTYNQIPAGSPFGGYK.N
24.
gi|194038349
Mass: 38243
Score:
PREDICTED: similar to SERPINA3-6 [Sus
Query Observed
Mr(expt)
Mr(calc)
249 480.7791
959.5436
959.5440
284 493.7793
985.5440
985.5444
458 558.8296 1115.6446 1115.6451
667 635.8668 1269.7190 1269.7180
820 717.3726 1432.7306 1432.7310
362
Queries matched: 13
emPAI: 0.51
scrofa]
-0.36
0
41
0.13
1
R.IDALYLPR.F
-0.36
0
47
0.022
1
K.QLVSLDPSK.N
-0.40
1
27
1.5
1
R.RIDALYLPR.F
0.82
0
38
0.11
1
R.GPTLTELLEGLK.F
-0.26
1
27
2.3
1
K.LLDEFRQDAQAK.F
12/9/2009
995
1071
1389
1440
25.
801.3885
845.4078
725.3738
773.7264
1600.7624
1688.8010
2173.0996
2318.1574
1600.7621
1688.8006
2173.1015
2318.1583
0.24
0.27
-0.90
-0.40
44
66
51
21
0.035
0.00026
0.0091
10
1
1
1
1
R.FSVSGDYQLEDTLK.Q
K.VFTHEADLSGVTGDNKLGVSK.V 1388 1391
R.TPVDPHAIVPSNTDFAFSLYK.Q
gi|2492797
Mass: 67837
Score: 352
Queries matched: 11
emPAI: 0.10
Query Observed
Mr(expt)
Mr(calc)
43 386.7229
771.4312
771.4313 -0.03
0
29
0.19 10
K.AACLLPK.L
46 395.2393
788.4640
788.4644 -0.41
0
31
0.51
1
K.LVTDLTK.V
110 424.2552
846.4958
846.4963 -0.56
1
32
0.33
1
R.LSQKFPK.A 109
165 449.7443
897.4740
897.4742 -0.18
0
37
0.14
1
R.LCVLHEK.T
184 457.2424
912.4702
912.4705 -0.27
0
33
0.35
1
K.YLYEVAR.R
227 474.2707
946.5268
946.5270 -0.14
0
38
0.23
2
K.LCTVATLR.E
501 575.3112 1148.6078 1148.6077
0.11
0
44
0.057
1
K.LVNEVTEFAK.T
503 576.2552 1150.4958 1150.4964 -0.52
0
52
0.003
1
K.EACFAEEGPK.F
930 513.9443 1538.8111 1538.8127 -1.03
1
27
2.1
2
R.LCVLHEKTPVSEK.V
1025 820.4720 1638.9294 1638.9305 -0.63
1
29
0.46
1
K.KVPQVSTPTLVEVSR.N
Mass: 68829
Score: 352
gi|109074537
PREDICTED: albumin [Macaca mulatta]
Mass: 68726
Score: 352
gi|126143530
hypothetical protein [Macaca fascicularis]
26.
0
0
1
0
Page 9 of 20
gi|31340900
Mass: 46175
Query
Observed
Mr(expt)
14
357.6982
713.3818
44
389.7207
777.4268
201
465.7662
929.5178
237
476.2608
950.5070
323
506.7948 1011.5750
464
561.3135 1120.6124
649
630.8555 1259.6964
1126
867.9542 1733.8938
1340 1037.0000 2071.9854
Queries matched: 11
Queries matched: 11
Score: 350
Queries matched: 12
emPAI: 0.15
clade A, member 3 [Bos taurus]
Mr(calc)
713.3820 -0.24
0
30
0.63
1
R.NSLQPR.R 15
777.4272 -0.49
0
26
2.1
1
K.IEELFK.Y
929.5182 -0.37
0
41
0.15
1
K.LTPETLTR.W
950.5073 -0.24
0
45
0.046
1
R.SLINDYVK.N
1011.5753 -0.24
0
26
2.2
1
R.IHELYLPK.F
1120.6128 -0.33
0
64 0.00039
1
K.DTQSIIFLGK.V
1259.6973 -0.65
0
30
0.61
1
R.GSTLTEILEGLK.F
1733.8948 -0.53
0
52
0.0086
1
K.SNYELNDILSQLGIR.K
2071.9851
0.17
0
37
0.22
1
gi|160332365
Mass: 46208
Score: 350
Queries matched: 12
gi|226373737
Mass: 46208
Score: 350
Queries matched: 12
27.
gi|119914036
Mass: 34009
Score: 349
Queries matched: 17
emPAI: 0.20
Query
Observed
Mr(expt)
Mr(calc)
14
357.6982
713.3818
713.3820 -0.24
0
30
0.63
1
R.NSLQPR.- 15
44
389.7207
777.4268
777.4272 -0.49
0
26
2.1
1
K.IEELFK.D
237
476.2608
950.5070
950.5073 -0.24
0
45
0.046
1
K.SLINDYVK.K
248
480.7627
959.5108
959.5110 -0.14
0
31
0.97
1
K.LTPEMLTR.W
591
407.2274 1218.6604 1218.6608 -0.36
1
35
0.38
1
667
635.8668 1269.7190 1269.7180
0.82
0
38
0.11
1
R.GPTLTEILEGLK.F
733
673.8692 1345.7238 1345.7241 -0.21
1
61 0.00087
1
K.IEELFKDLSPR.T 732 734
1325 1021.9890 2041.9634 2041.9633
0.09
0
46
0.03
1
R.VLYSSEAFPTNFGDPEAAK.S 1326
1340 1037.0000 2071.9854 2071.9851
0.17
0
37
0.22
1
28.
gi|157164095
Mass: 55914
Score: 346
Queries matched: 15
emPAI: 0.67
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
826
719.3660 1436.7174 1436.7194 -1.38
0
25
3.5
1
K.VHHTMGGLNINTK.A 827
1039
550.9494 1649.8264 1649.8274 -0.63
0
47
0.026
1
K.YIDQITHAGGHSVPR.S 1038
1047
832.4309 1662.8472 1662.8465
0.45
0
45
0.035
1
K.GVDEDFGKPVVATTTK.G
1170
597.9880 1790.9422 1790.9415
0.40
1
26
2.2
1
K.KGVDEDFGKPVVATTTK.G
1353 1053.5140 2105.0134 2105.0212 -3.67
0
(26)
3
1
1362 1061.5150 2121.0154 2121.0161 -0.30
0
34
0.4
1
1416
750.7098 2249.1076 2249.1110 -1.54
1
21
11
1
R.TGGNSVINGGIFAVPNSDMQKK.A
1438 1159.5850 2317.1554 2317.1550
0.19
0
74 4.4e-005
1
R.ITPETDSTNQPGSTAGALLAAFR.A 1437 1439
1511
984.1854 2949.5344 2949.5369 -0.85
1
32
0.36
1
R.SLQTEVGSGAGIVLPMTATFEKLEGASIK.K
1517 1019.8330 3056.4772 3056.4808 -1.18
0
(26)
2.3
1
1518 1025.1650 3072.4732 3072.4757 -0.82
0
42
0.06
1
29.
gi|181402
Mass: 65825
Score: 341
Query Observed
Mr(expt)
Mr(calc)
261 487.2691
972.5236
972.5240
438 554.2749 1106.5352 1106.5356
643 627.8074 1253.6002 1253.6001
722 665.3666 1328.7186 1328.7187
856 730.9028 1459.7910 1459.7922
869 738.3962 1474.7778 1474.7780
Queries matched: 6
emPAI: 0.28
-0.33
0
46
0.047
1
-0.32
0
51
0.012
1
0.16
0
70 0.00011
1
-0.05
0
63 0.00061
1
-0.80
0
54
0.0038
1
-0.09
0
58
0.0018
1
Peptide
K.IEISELNR.V
K.AQYEEIAQR.S
R.GFSSGSAVVSGGSR.R
R.NLDLDSIIAEVK.A
K.VDLLNQEIEFLK.V
R.FLEQQNQVLQTK.W
12/9/2009
Page 10 of 20
gi|47132620
Mass: 65393
Score: 341
Queries matched: 6
Mass: 65944
Score: 341
Queries matched: 6
gi|148727309
30.
gi|51831151
Mass: 49407
Score: 335
Queries matched: 11
emPAI: 0.29
immunoglobulin mu heavy chain constant chain secreted form [Equus caballus]
Query
Observed
Mr(expt)
Mr(calc)
289
495.2742
988.5338
988.5342 -0.32
0
39
0.33
1
K.EISVSWLR.D
317
505.2529 1008.4912 1008.4916 -0.39
0
38
0.24
1
K.ESWPVTYK.V
439
554.2892 1106.5638 1106.5641 -0.27
0
34
0.58
1
R.ESASVTCLVK.G
507
578.3273 1154.6400 1154.6407 -0.61
0
20
4.5
1
K.QAISRPDAVAK.H
573
604.3107 1206.6068 1206.6067
0.12
0
56 0.0035
1
R.LVCQATGFSPK.E
594
611.3183 1220.6220 1220.6223 -0.24
0
(36)
0.39
1
R.LVCQATGFSPK.E
755
681.8796 1361.7446 1361.7456 -0.68
0
20
7.4
1
K.HPPSVYVLPPTR.E
1013
811.4712 1620.9278 1620.9279 -0.06
0
25
0.58
1
K.IFAIPPSFAGIFLTK.S
1033
824.4305 1646.8464 1646.8457
0.45
0
33
0.61
1
K.GFSPPDVFVQWLQK.G
1221
933.4680 1864.9214 1864.9241 -1.40
0
45
0.048
1
K.VTSMLTITESDWLNQK.V
1348 1045.5060 2088.9974 2088.9964
0.51
0
25
4
1
R.DGKPVESGFTTEEVQPQNK.E
31.
gi|157165649
Mass: 40295
Score: 328
Queries matched: 9
emPAI: 0.37
Query Observed
Mr(expt)
Mr(calc)
42 385.7076
769.4006
769.4010 -0.48
0
25
1.8
1
R.AEFYLK.G
257 485.7184
969.4222
969.4226 -0.34
0
31
0.44
1
R.FSDQMGSAK.D
455 557.8445 1113.6744 1113.6757 -1.16
1
25
1.2
1
K.AILESGKLVGK.C
723 668.3137 1334.6128 1334.6136 -0.60
0
29
1
2
R.DNSILGCGTGDVK.A
843 727.3665 1452.7184 1452.7184
0.07
0
52
0.0056
1
K.AIYTWVANTMQR.D
875 740.8455 1479.6764 1479.6769 -0.32
0
67 0.00017
1
K.EFSYDYVSTELK.908 759.3852 1516.7558 1516.7562 -0.24
0
45
0.032
1
K.GYGWIPVDPADVTK.V
918 764.9202 1527.8258 1527.8256
0.14
1
54
0.0034
1
K.ALEIIGNTKGDLER.A 917
32.
gi|126165236
Mass: 46368
Score: 327
Queries matched: 12
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
14 357.6982
713.3818
713.3820 -0.24
0
30
0.63
1
R.NSLQPR.R 15
44 389.7207
777.4268
777.4272 -0.49
0
26
2.1
1
K.IEELFK.Y
201 465.7662
929.5178
929.5182 -0.37
0
41
0.15
1
K.LTPETLTR.W
237 476.2608
950.5070
950.5073 -0.24
0
45
0.046
1
R.SLINDYVK.N
323 506.7948 1011.5750 1011.5753 -0.24
0
26
2.2
1
R.IHELYLPK.F
464 561.3135 1120.6124 1120.6128 -0.33
0
64 0.00039
1
K.DTQSIIFLGK.V
591 407.2274 1218.6604 1218.6608 -0.36
1
35
0.38
1
667 635.8668 1269.7190 1269.7180
0.82
0
38
0.11
1
R.GPTLTEILEGLK.F
714 656.8168 1311.6190 1311.6208 -1.30
0
22
6
1
K.HTEQAEFHVSK.N
33.
gi|194038351
Mass: 46968
Score: 321
Queries matched: 12
emPAI: 0.31
Query Observed
Mr(expt)
Mr(calc)
32 367.7258
733.4370
733.4374 -0.51
0
32
0.26
1
K.IVDLFK.Q
198 460.7873
919.5600
919.5603 -0.29
0
40
0.022
1
R.ITALHLPR.F 197
567 602.8375 1203.6604 1203.6612 -0.59
1
53 0.0069
1
K.LLDKFVQDAR.E
649 630.8555 1259.6964 1259.6973 -0.65
0
30
0.61
1
R.GSTLTELLEGLK.F
941 779.4194 1556.8242 1556.8232
0.68
0
40
0.079
1
R.MAAVEAQLLPETLR.R
961 792.8813 1583.7480 1583.7467
0.83
0
47
0.02
1
K.DSDAAVEFINNYVK.N
1091 566.6505 1696.9297 1696.9294
0.17
1
(23)
3.1
1
R.MAAVEAQLLPETLRR.W
1108 571.9819 1712.9239 1712.9243 -0.25
1
25
2.2
1
R.MAAVEAQLLPETLRR.W
1132 872.9336 1743.8526 1743.8540 -0.79
0
55
0.004
1
K.VFSHQADLSGITDQAR.L 1133 1134
34.
gi|157165374
Mass: 33156
Score: 308
Queries matched: 6
emPAI: 0.46
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
520
582.7856 1163.5566 1163.5571 -0.37
1
25
4.4
1
K.FDGAKDENLR.S
774
692.8331 1383.6516 1383.6518 -0.11
0
80 8.9e-006
1
K.DSGLTSDLSSFAGK.K
903
756.8802 1511.7458 1511.7468 -0.60
1
20
13
1
K.DSGLTSDLSSFAGKK.Y
1053
838.3999 1674.7852 1674.7850
0.17
0
51
0.0063
1
1116
860.0074 1718.0002 1718.0018 -0.92
0
36
0.029
1
1311 1005.9920 2009.9694 2009.9694
0.01
0
95 3.4e-007
1
35.
gi|157165273
Mass: 40059
Score:
Query Observed
Mr(expt)
Mr(calc)
258 486.7552
971.4958
971.4964
265 487.7794
973.5442
973.5444
298 498.2633
994.5120
994.5124
469 563.7834 1125.5522 1125.5529
492 571.7809 1141.5472 1141.5478
504 576.3082 1150.6018 1150.6022
706 653.3378 1304.6610 1304.6612
290
Queries matched: 9
emPAI: 0.27
-0.54
0
42
0.048
1
-0.16
1
56 0.0031
1
-0.33
0
21
10
3
-0.54
0
(39)
0.15
1
-0.46
0
51 0.0077
1
-0.35
0
27
2.7
1
-0.11
1
38
0.22
1
Peptide
K.ISDYAYIK.T
K.SVDDIKGLK.I
K.VFDPYNIK.I
K.SPFAMLDFAK.S
K.SPFAMLDFAK.S
R.IPGFGGEIYAK.L
K.NAYEKLPDDLK.A
12/9/2009
803
1158
710.9015
889.8999
1419.7884
1777.7852
1419.7874
1777.7835
0.73
0.98
1
0
27
28
1.3
0.98
Page 11 of 20
1
1
K.IRIPGFGGEIYAK.L
K.SGQYDIGYTASYYYK.G
36.
gi|47523270
Mass: 46616
alpha-1-antichymotrypsin 2
Query Observed
Mr(expt)
20 360.1913
718.3680
32 367.7258
733.4370
59 402.2168
802.4190
202 465.7718
929.5290
263 487.7320
973.4494
516 580.8387 1159.6628
645 629.3656 1256.7166
667 635.8668 1269.7190
37.
gi|157165109
Mass: 35585
Score: 279
Queries matched: 8
emPAI: 0.31
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
286
494.2766
986.5386
986.5396 -0.99
0
28
2.6
1
R.INELLNSGK.A
307
501.3246 1000.6346 1000.6355 -0.80
0
57 7.4e-005
1
R.VLLMLALTK.T
356
517.7686 1033.5226 1033.5233 -0.62
0
33
0.94
1
K.IQEFFLTH.368
527.3188 1052.6230 1052.6230
0.07
0
50
0.0023
1
K.LIAAVPDINK.I
401
542.2690 1082.5234 1082.5244 -0.91
0
38
0.12
1
K.TNTTGVDTFK.S 402
668
636.3602 1270.7058 1270.7067 -0.70
0
32
0.47
1
K.SDKPVVLVGAMR.N
1430 1144.5960 2287.1774 2287.1808 -1.47
0
41
0.079
1
38.
gi|157163977
Mass: 36762
Score: 274
Queries matched: 9
emPAI: 0.30
Query Observed
Mr(expt)
Mr(calc)
41 385.1974
768.3802
768.3806 -0.50
0
33
0.1
1
K.AADFFAK.R
95 421.2262
840.4378
840.4382 -0.37
0
24
2.1
1
K.FYDVIGK.E
225 473.7509
945.4872
945.4879 -0.72
1
47
0.032
1
K.RAEELSGGK.I
473 564.3141 1126.6136 1126.6135
0.14
0
34
0.37
1
K.FAHVVAASTPK.G 472
615 619.8040 1237.5934 1237.5938 -0.32
0
24
4.1
2
K.QALSEATAYER.E
737 675.3477 1348.6808 1348.6809 -0.04
0
(59) 0.0017
1
K.LGNVQMAAPSFSK.F
759 683.3452 1364.6758 1364.6758
0.02
0
64 0.00057
1
K.LGNVQMAAPSFSK.F
1028 821.9545 1641.8944 1641.8938
0.42
1
48
0.0086
1
39.
gi|229552
Mass: 66088
albumin
Query Observed
Mr(expt)
36 379.7151
757.4156
43 386.7229
771.4312
46 395.2393
788.4640
110 424.2552
846.4958
165 449.7443
897.4740
518 582.3187 1162.6228
550 597.3046 1192.5946
930 513.9443 1538.8111
1025 820.4720 1638.9294
40.
gi|126165264
SERPINA3-8 [Bos
Query Observed
32 367.7258
51 396.2422
237 476.2608
464 561.3135
1151 887.4476
Score: 281
Queries matched: 8
emPAI: 0.07
[Sus scrofa]
Mr(calc)
718.3683 -0.40
0
31
1.2
1
R.MAAVEAK.L
733.4374 -0.51
0
32
0.26
1
K.IVDLFK.K
802.4193 -0.37
0
32
2
1
R.VPMMGIR.A
929.5294 -0.38
0
48
0.023
2
R.SSNLLQLR.L
973.4505 -1.08
0
31
0.46
1
K.FSISSDYR.L
1159.6641 -1.09
0
37
0.12
1
R.WIVELYLPK.F
1256.7169 -0.19
0
32
0.38
1
K.VVLVNYIYFK.A
1269.7180
0.82
0
38
0.11
1
R.GPTLTELLEGLK.F
Mass: 46888
taurus]
Mr(expt)
733.4370
790.4698
950.5070
1120.6124
1772.8806
Score: 261
Mr(calc)
757.4156
771.4313
788.4644
846.4963
897.4742
1162.6234
1192.5949
1538.8127
1638.9305
Queries matched: 11
0.02
0
(31)
0.3
2
-0.03
0
36
0.046
3
-0.41
0
31
0.51
1
-0.56
1
32
0.33
1
-0.18
0
37
0.14
1
-0.45
0
31
0.77
1
-0.18
1
35
0.43
1
-1.03
1
29
1.3
1
-0.63
1
29
0.46
1
Score: 249
Mr(calc)
733.4374
790.4701
950.5073
1120.6128
1772.8792
emPAI: 0.05
Queries matched: 5
Peptide
K.GACLLPK.I
K.GACLLPK.I
K.LVTDLTK.V
R.LSQKFPK.A 109
R.LCVLHEK.T
K.LVNELTEFAK.T
-.DTHKSEIAHR.F 548
R.LCVLHEKTPVESK.V
R.KVPQVSTPTLVEVSR.S
emPAI: 0.15
ppm Miss Score
Expect Rank
-0.51
0
32
0.26
1
-0.33
1
22
9
1
-0.24
0
45
0.046
1
-0.33
0
64
0.00039
1
0.80
0
87 1.8e-006
1
gi|151554250
Mass: 46935
Score: 249
Peptide
K.IVDLFK.D
K.LLDKFR.E
K.SLINDYVK.N
K.DTQSIIFLGK.V
Queries matched: 5
41.
gi|157164952
Mass: 68422
Score: 247
Queries matched: 7
emPAI: 0.15
arylsulfotransferase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
67 405.7267
809.4388
809.4395 -0.86
0
27
1.2
1
R.HQDAVIK.I
274 490.2638
978.5130
978.5134 -0.40
0
25
5.7
1
K.QGGQVISYK.V
669 637.3323 1272.6500 1272.6503 -0.16
0
39
0.15
1
R.LYYVNNFVNK.T
925 769.3853 1536.7560 1536.7572 -0.77
1
31
1.5
1
K.TVEQIWEYGKER.G
987 799.8359 1597.6572 1597.6580 -0.45
0
38
0.031
1
K.QNDDGAMTWGYGQR.Y
1192 918.4800 1834.9454 1834.9465 -0.56
0
66 0.00027
1
K.IYAPAIYLESAGTPNQK.G
1205 925.4354 1848.8562 1848.8539
1.29
0
21
8.4
1
K.MTNAMGYQAFPFSLQK.A
42.
gi|157164517
Mass: 96149
Score: 240
Queries matched: 6
emPAI: 0.22
Query Observed
Mr(expt)
Mr(calc)
498 573.3316 1144.6486 1144.6492 -0.47
0
44
0.039
1
K.AGALFLIPESK.S
12/9/2009
563
588
928
936
938
43.
601.3431
608.8242
769.8857
775.8755
777.8822
1200.6716
1215.6338
1537.7568
1549.7364
1553.7498
1200.6714
1215.6347
1537.7559
1549.7372
1553.7508
gi|122614
Mass: 15998
Score: 234
Query
Observed
Mr(expt)
Mr(calc)
502
575.3405 1148.6664 1148.6666
656
633.4186 1264.8226 1264.8231
671
637.8663 1273.7180 1273.7183
812
713.8461 1425.6776 1425.6776
1308 1000.4650 1998.9154 1998.9146
1315 1008.4620 2014.9094 2014.9095
1444
776.0724 2325.1954 2325.1964
0.23
-0.69
0.64
-0.50
-0.60
53 0.0054
41
0.073
43
0.061
58 0.0017
(41) 0.093
1
1
1
1
1
R.GLSEKDVLAAAK.T
K.ATDDVIQEVVK.Y
K.LTPALTYNMGTNSR.T
R.TGEASPYATLAGQER.V
K.LTPALTYNMGTNSR.T
Queries matched: 8
emPAI: 1.16
-0.11
0
34
0.14
1
K.VVAGVANALAHK.Y
-0.35
0
52 0.00014
1
R.LLGNVLVVVLAR.H
-0.19
0
29
0.82
1
R.LLVVYPWTQR.F 672
0.04
0
26
2.7
1
K.DFTPELQASYQK.V
0.43
0
(45)
0.023
1
-0.03
0
65 0.00023
1
-0.46
1
27
1.7
1
K.AAVLALWDKVNEEEVGGEALGR.L
Mass: 16129
Score: 234
gi|255683515
44.
1
0
0
0
0
Page 12 of 20
Queries matched: 8
gi|122411
Mass: 15236
Score: 231
Queries matched: 9
emPAI: 0.83
Query Observed
Mr(expt)
Mr(calc)
358 521.2753 1040.5360 1040.5365 -0.43
0
(37)
0.16
1
R.MFLGFPTTK.T
371 529.2728 1056.5310 1056.5314 -0.35
0
39
0.12
1
R.MFLGFPTTK.T 372
496 573.3297 1144.6448 1144.6452 -0.28
1
66 0.00023
1
M.VLSAADKTNVK.A 497
663 634.8585 1267.7024 1267.7024
0.06
0
44
0.025
1
K.FLSSVSTVLTSK.Y
904 505.9109 1514.7109 1514.7113 -0.31
0
53
0.0043
1
K.VGGHAGEYGAEALER.M 905
1189 611.9686 1832.8840 1832.8846 -0.34
0
29
1.4
1
gi|230479
Mass: 15105
Score: 231
Queries matched: 9
gi|230632
Mass: 15105
Score: 231
Queries matched: 9
gi|313500
Mass: 15236
Score: 231
Queries matched: 9
gi|24158717
Mass: 15105
Score: 231
Queries matched: 9
45.
gi|136429
Mass: 24394
Score: 225
Query
Observed
Mr(expt)
Mr(calc)
100
421.7581
841.5016
841.5022
359
523.2853 1044.5560 1044.5564
1398 1106.0550 2210.0954 2210.0967
1426
761.7311 2282.1715 2282.1729
Queries matched: 11
emPAI: 0.90
ppm Miss Score
Expect Rank
-0.60
0
56
0.0041
1
-0.29
0
40
0.13
1
-0.58
0
98 1.8e-007
1
-0.62
0
32
0.69
1
Peptide
R.VATVSLPR.S 99 101
K.LSSPATLNSR.V 360
K.IITHPNFNGNTLDNDIMLIK.L 1428
gi|494360
Mass: 23458
Score: 225
Queries matched: 11
gi|999627
Mass: 8814
Score: 225
Queries matched: 11
gi|1942351
Mass: 13284
Score: 225
Queries matched: 11
gi|2914482
Mass: 23460
Score: 225
Queries matched: 11
gi|3318722
Mass: 23457
Score: 225
Queries matched: 11
gi|110590762
Mass: 23458
Score: 225
Queries matched: 11
gi|157878102
Mass: 24157
Score: 225
Queries matched: 11
gi|242253868
Mass: 25865
Score: 225
Queries matched: 11
46.
gi|27807517
Mass: 42533
Score: 223
Queries matched: 8
emPAI: 0.25
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
180 454.7454
907.4762
907.4763 -0.08
0
38
0.27
1
R.DTQYLLR.T 179
325 507.7189 1013.4232 1013.4236 -0.39
0
72 1.6e-005
1
R.ADFSGMSSGR.G
349 515.7162 1029.4178 1029.4186 -0.70
0
(27)
0.25
1
R.ADFSGMSSGR.G
538 590.3602 1178.7058 1178.7063 -0.40
0
51
0.0016
1
R.LVLVNAIYFK.G 539
738 675.8487 1349.6828 1349.6835 -0.52
0
40
0.13
1
K.NVEKPVQMMFK.K
813 714.3690 1426.7234 1426.7238 -0.27
0
22
6.6
1
R.DLLPANSVNPMTR.L
47.
gi|1942778
Mass: 15089
Score: 221
Queries matched: 9
emPAI: 0.84
Query Observed
Mr(expt)
Mr(calc)
12/9/2009
358
371
496
663
893
1189
521.2753
529.2728
573.3297
634.8585
750.3660
611.9686
1040.5360
1056.5310
1144.6448
1267.7024
1498.7174
1832.8840
Mass:
gi|146149083
gi|146149272
Mass:
1040.5365
1056.5314
1144.6452
1267.7024
1498.7164
1832.8846
-0.43
-0.35
-0.28
0.06
0.67
-0.34
15220
Score: 221
caballus]
15220
Score: 221
caballus]
0
0
1
0
0
0
(37)
0.16
39
0.12
66 0.00023
44
0.025
43
0.044
29
1.4
1
1
1
1
1
1
Page 13 of 20
R.MFLGFPTTK.T
R.MFLGFPTTK.T 372
-.VLSAADKTNVK.A 497
K.FLSSVSTVLTSK.Y
K.VGGHAGEFGAEALER.M 892
Queries matched: 9
Queries matched: 9
48.
gi|194225326
Mass: 46896
Score: 220
Queries matched: 5
emPAI: 0.23
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
243 478.7557
955.4968
955.4975 -0.63
0
29
0.69
1
R.HLEDTLTK.G
480 567.3193 1132.6240 1132.6240
0.01
0
48
0.081
1
K.GILATFLENR.H
753 679.8669 1357.7192 1357.7201 -0.64
0
72 6.8e-005
1
K.GTEAAGATIVEAIR.T 752
984 799.4223 1596.8300 1596.8300
0.03
0
71 6.3e-005
1
K.IAPNLADFAFSLYR.H
49.
gi|126352628
Mass: 78044
Score:
transferrin [Equus caballus]
Query Observed
Mr(expt)
Mr(calc)
149 439.7400
877.4654
877.4658
233 475.7617
949.5088
949.5094
339 510.7952 1019.5758 1019.5764
425 549.2554 1096.4962 1096.4978
837 725.3464 1448.6782 1448.6783
855 730.3737 1458.7328 1458.7323
206
Queries matched: 7
emPAI: 0.09
-0.35
0
37
0.34
4
-0.53
0
41
0.092
1
-0.50
0
21
7.7
1
-1.40
0
21
5.2
1
-0.06
0
62 0.00065
1
0.37
0
22
6.6
1
Peptide
K.DSALGFLR.I
R.APNHAVVSR.K
K.YLTAVANLR.Q
R.YYGYTGAFR.C
K.SSSDPDLTWNSLK.G 836
R.TAVPNLCQLCVGK.G
50.
gi|15027001
Mass: 36537
Query
Observed
Mr(expt)
85
416.7294
831.4442
90
417.2309
832.4472
481
567.8126 1133.6106
932
771.3619 1540.7092
1156
889.4297 1776.8448
1374 1071.9970 2141.9794
Score: 201
Queries matched: 6
emPAI: 0.19
Mr(calc)
831.4450 -0.93
0
28
3.8
1
K.LTVETNR.W
832.4477 -0.50
0
33
1.1
1
K.DVLMISR.T
1133.6115 -0.71
0
30
1.4
1
R.TNVSVTCLVK.D
1540.7086
0.44
0
23
3.7
1
K.DFYPTDIDIEWK.S
1776.8465 -0.91
0
29
1.6
1
2141.9793
0.06
0
58 0.0012
1
51.
gi|15027003
Mass: 36471
Query
Observed
Mr(expt)
85
416.7294
831.4442
90
417.2309
832.4472
678
639.8619 1277.7092
932
771.3619 1540.7092
1156
889.4297 1776.8448
1374 1071.9970 2141.9794
Score: 198
Queries matched: 6
emPAI: 0.19
Mr(calc)
831.4450 -0.93
0
28
3.8
1
K.LTVETNR.W
832.4477 -0.50
0
33
1.1
1
K.DVLMISR.T
1277.7092
0.06
0
26
1.4
1
K.VNNQALPAPVQK.T
1540.7086
0.44
0
23
3.7
1
K.DFYPTDIDIEWK.S
1776.8465 -0.91
0
29
1.6
1
2141.9793
0.06
0
58 0.0012
1
Mass: 35699
Score: 198
Queries matched: 6
gi|42528291
immunoglobulin gamma 7 heavy chain [Equus caballus]
52.
gi|194038345
Mass: 38368
Score: 195
Queries matched: 5
emPAI: 0.18
Query Observed
Mr(expt)
Mr(calc)
17 357.7416
713.4686
713.4687 -0.08
0
22
3.4
1
K.IVELIK.R
464 561.3135 1120.6124 1120.6128 -0.33
0
64 0.00039
1
R.DTQSIIFLGK.V
667 635.8668 1269.7190 1269.7180
0.82
0
38
0.11
1
R.GPTLTELLEGLK.F
794 706.8830 1411.7514 1411.7500
1.03
0
40
0.088
1
R.FDSPFLFATVLR.D
809 475.6155 1423.8247 1423.8259 -0.87
0
31
0.22
1
R.TLNRPSNLLQLR.L
53.
gi|47523844
Mass: 47164
Score: 192
Queries matched: 4
emPAI: 0.14
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
329 508.3109 1014.6072 1014.6073 -0.08
0
32
0.49
1
K.SLLGNLGITK.V 330
984 799.4223 1596.8300 1596.8300
0.03
0
71 6.3e-005
1
R.IAPNLADFAFSLYR.Q
1284 982.4780 1962.9414 1962.9422 -0.39
0
89 1.3e-006
1
54.
gi|291470
Mass: 19537
Query Observed
Mr(expt)
350 516.2613 1030.5080
511 579.7765 1157.5384
870 738.8679 1475.7212
Score: 181
Mr(calc)
1030.5083
1157.5387
1475.7216
Queries matched: 3
emPAI: 0.37
ppm Miss Score
Expect Rank
-0.27
0
65
0.00043
1
-0.19
0
26
1.4
1
-0.26
0
89 1.4e-006
1
Peptide
K.YAASSYLTR.T
K.SYSSVSCQVK.H
K.VNDAVTTDGVQTTR.S
12/9/2009
Page 14 of 20
gi|291474
Mass: 17423
Score: 181
Queries matched: 3
gi|291478
Mass: 22654
Score: 181
Queries matched: 3
Mass: 24186
Score: 181
Queries matched: 3
gi|9858133
gi|194214037
Mass: 17257
Score: 181
Queries matched: 3
Mass: 15452
Score: 181
Queries matched: 3
gi|194214039
55.
gi|154175426
Mass: 28890
Score: 179
Queries matched: 4
emPAI: 0.24
Query Observed
Mr(expt)
Mr(calc)
219 471.2925
940.5704
940.5706 -0.12
0
24
3.4
1
K.GLIVGVANAK.S
256 485.2901
968.5656
968.5655
0.19
0
45
0.021
1
R.VNAISAGPIK.T
440 554.2935 1106.5724 1106.5720
0.39
0
51
0.012
1
K.TLAASGIGDFR.M
881 746.4061 1490.7976 1490.7981 -0.28
0
59 0.0011
1
K.EGGSVLTLTYLGGPK.F
Mass: 29118
Score: 179
Queries matched: 4
gi|157165165
gi|223038888
Mass: 29342
Score: 179
Queries matched: 4
gi|255323509
Mass: 29040
Score: 179
Queries matched: 4
56.
gi|158604891
Mass: 85776
Score: 177
Queries matched: 4
emPAI: 0.08
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase [Campylobacter concisus 13826]
Expect Rank Peptide
Query
Observed
Mr(expt)
Mr(calc)
ppm Miss Score
157
441.7866
881.5586
881.5586
0.08
0
28
0.25
1
K.ALLEVLPK.E
631
624.8085 1247.6024 1247.6033 -0.71
0
25
3.4
1
K.YEENLNPEIK.S
713
656.3792 1310.7438 1310.7446 -0.54
0
75 1.4e-005
1
R.VPSVEEIVAQIK.A
1344 1040.0590 2078.1034 2078.1048 -0.66
0
50
0.0066
1
K.YGILPTTTIGSFPQTVDLR.V
57.
gi|194225324
Mass: 46743
Score: 172
Queries matched: 3
emPAI: 0.15
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
243 478.7557
955.4968
955.4975 -0.63
0
29
0.69
1
R.HLEDTLTK.G
984 799.4223 1596.8300 1596.8300
0.03
0
71 6.3e-005
1
K.IAPNLADFAFSLYR.H
1140 879.9598 1757.9050 1757.9047
0.20
0
72 7.3e-005
1
R.QADLSGITEEAPLTVSK.A
58.
gi|157164859
Mass: 27476
Score: 170
Queries matched: 6
emPAI: 0.58
Query Observed
Mr(expt)
Mr(calc)
561 601.3252 1200.6358 1200.6350
0.72
0
58 0.0027
1
K.GNTELLEAINK.A 560
652 632.8300 1263.6454 1263.6459 -0.39
0
35
0.4
1
K.NGATVVVADGFSK.G
999 803.3972 1604.7798 1604.7794
0.25
0
43
0.059
1
K.ADVVFNQVSINEDR.K
1117 860.4530 1718.8914 1718.8913
0.11
0
34
0.42
1
1127 868.4497 1734.8848 1734.8862 -0.77
0
(34)
0.48
1
59.
gi|157164059
Mass: 91250
Score: 167
Queries matched: 3
emPAI: 0.07
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
618 621.7853 1241.5560 1241.5564 -0.28
0
56
0.0016
1
K.EFYADAAEQAK.G
703 652.3482 1302.6818 1302.6819 -0.08
0
84 4.9e-006
1
R.VSWETALDLAAK.A
937 776.9505 1551.8864 1551.8872 -0.48
0
27
0.73
1
K.LSDIVLPVALEVER.N
60.
gi|27807349
Mass: 51691
Query Observed
Mr(expt)
370 528.2613 1054.5080
453 556.8031 1111.5916
513 579.8040 1157.5934
1057 839.9360 1677.8574
61.
gi|223039637
Mass: 39873
Score: 163
Queries matched: 5
emPAI: 0.17
Query Observed
Mr(expt)
Mr(calc)
42 385.7076
769.4006
769.4010 -0.48
0
25
1.8
1
R.AEFYLK.G
455 557.8445 1113.6744 1113.6757 -1.16
1
25
1.2
1
K.AILESGKLVGK.C
718 661.3063 1320.5980 1320.5980
0.03
0
61 0.00058
1
R.DNSVIGCGTGDVK.A
723 668.3137 1334.6128 1334.6136 -0.60
0
(47)
0.016
1
R.DNSVIGCGTGDVK.A
843 727.3665 1452.7184 1452.7184
0.07
0
52
0.0056
1
K.AIYTWVANTMQR.D
62.
gi|158605017
Mass: 30046
Score: 163
Queries matched: 6
emPAI: 0.23
Query Observed
Mr(expt)
Mr(calc)
Score: 166
Queries matched: 5
emPAI: 0.20
Mr(calc)
1054.5084 -0.30
0
20
14
1
K.YPVASFTDR.T
1111.5914
0.26
0
55
0.0035
1
R.LYQDFSVLK.K
1157.5928
0.56
0
25
3.8
1
R.LLDSLPEDTR.L
1677.8573
0.06
0
66 0.00035
1
R.SAEAVLGEALTDFSLR.L 1056
12/9/2009
26
312
716
989
994
1005
363.6945
504.2738
657.8379
799.8979
800.9383
807.8947
725.3744
1006.5330
1313.6612
1597.7812
1599.8620
1613.7748
725.3748
1006.5335
1313.6615
1597.7810
1599.8620
1613.7759
-0.53
-0.44
-0.21
0.14
0.00
-0.68
0
1
1
0
0
0
21
29
45
(30)
23
44
1.2
1.6
0.032
1.1
4
0.042
1
1
1
1
1
1
Page 15 of 20
K.FIGDFK.L
K.VEKEFVEK.G
R.GTYIKYEANQK.G
R.NAVMYNDFVVIADK.S
K.LLDKPLFTPDANTR.K
R.NAVMYNDFVVIADK.S
63.
gi|157164610
Mass: 46159
Score: 155
Queries matched: 5
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
140 433.7420
865.4694
865.4698 -0.39
0
20
1.6
1
R.SFQFPLK.V
562 601.3303 1200.6460 1200.6463 -0.17
0
33
0.62
1
K.ELGGQSSLGVVR.L
624 623.3636 1244.7126 1244.7129 -0.18
0
29
0.82
1
K.TIVSGGLNIFPK.W
715 657.3397 1312.6648 1312.6663 -1.13
0
24
4.5
1
R.FSSDGQSVVFIK.E
1294 990.4570 1978.8994 1978.9021 -1.32
0
49
0.012
1
K.NNNSVSTFENYVVYSSR.E
64.
gi|157164268
Mass: 36935
ketol-acid reductoisomerase
Query Observed
Mr(expt)
741 676.8839 1351.7532
1014 813.4290 1624.8434
1230 940.9371 1879.8596
65.
gi|123506
Mass: 8353
Score: 150
Queries matched: 4
emPAI: 1.87
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
Expect Rank Peptide
280 492.3031
982.5916
982.5923 -0.70
0
45
0.011
1
R.ALGILNLNR.N
522 583.7663 1165.5180 1165.5186 -0.46
0
30
0.77
1
R.SYPSNATCPR.T
688 644.3448 1286.6750 1286.6765 -1.12
0
74 4.1e-005
1
R.NLQLILNSCGR.S
699 651.3533 1300.6920 1300.6921 -0.06
0
(40)
0.13
1
R.NLQLILNSCGR.S
Score: 151
Queries matched: 3
emPAI: 0.29
Mr(calc)
1351.7533 -0.06
0
29
0.7
1
K.APANIDVIMIAPK.A
1624.8420
0.87
0
67 0.00018
1
1879.8588
0.45
0
55
0.0028
1
gi|5019730
Mass: 12474
Score: 150
Queries matched: 4
gi|76782253
Mass: 12343
Score: 150
Queries matched: 4
66.
gi|2232299
Mass: 47885
Score: 143
Queries matched: 5
Query Observed
Mr(expt)
Mr(calc)
439 554.2892 1106.5638 1106.5641 -0.27
0
34
0.58
1
658 633.8088 1265.6030 1265.6040 -0.78
0
37
0.24
1
1016 817.4281 1632.8416 1632.8413
0.23
0
41
0.11
1
1159 892.4922 1782.9698 1782.9702 -0.21
0
(22)
4.5
1
1176 600.6617 1798.9633 1798.9651 -1.04
0
32
0.58
1
Peptide
R.ESASVTCLVK.G
K.ALETHTYFER.H
K.GFAPADVFVQWLQR.G
gi|3834667
Mass: 48183
Score: 143
Queries matched: 5
gi|24496448
Mass: 65621
Score: 143
Queries matched: 5
Mass: 49106
Score: 143
Queries matched: 5
gi|33413902
gi|34538498
Mass: 49909
Score: 143
Queries matched: 5
67.
gi|194216172
Mass: 68222
Score: 141
Queries matched: 3
emPAI: 0.10
PREDICTED: similar to Alpha-1B-glycoprotein precursor (Alpha-1-B glycoprotein) [Equus caballus]
Query Observed
Mr(expt)
Mr(calc)
397 538.2723 1074.5300 1074.5305 -0.47
0
69 0.00014
1
R.VGDTEASAVAR.A
454 557.8034 1113.5922 1113.5931 -0.76
0
44
0.043
1
R.ALWSGAVTPGR.D
1499 900.1044 2697.2914 2697.2923 -0.33
0
28
1.6
1
R.EGDDQFLEVAEAPEDVVVTFPVHR.A
68.
gi|157165065
Mass: 77441
Score: 141
Queries matched: 3
emPAI: 0.04
tRNA synthetase, class II [Campylobacter concisus 13826]
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
491 571.7685 1141.5224 1141.5226 -0.16
0
22
4.3
3
R.FNFSPDLMR.A
701 651.8550 1301.6954 1301.6979 -1.91
0
29
1.5
1
R.IPATSSLQFPNK.F
823 718.4061 1434.7976 1434.7970
0.47
0
89 5.5e-007
1
K.IGIEGIYSLLETK.K
69.
gi|154175542
Mass: 22096
Score: 139
Queries matched: 3
emPAI: 0.53
Query Observed
Mr(expt)
Mr(calc)
569 602.8433 1203.6720 1203.6724 -0.29
0
34
0.28
1
R.HAVINDLPLGR.N
710 654.3510 1306.6874 1306.6881 -0.52
1
35
0.36
1
R.GSFLLDKDGTVR.H
868 737.8987 1473.7828 1473.7827
0.07
0
70 0.00012
1
R.GFDVLLEDAGVALR.G
12/9/2009
Page 16 of 20
gi|157164012
Mass: 22011
Score: 139
Queries matched: 3
gi|255321903
Mass: 21900
Score: 139
Queries matched: 3
2-Cys peroxiredoxin BAS1 [Campylobacter showae RM3277]
70.
gi|157164319
Mass: 38441
Score: 131
Queries matched: 3
emPAI: 0.18
Query Observed
Mr(expt)
Mr(calc)
644 629.3254 1256.6362 1256.6361
0.14
0
35
0.3
1
K.DTTAHIQSSGIK.T
728 671.3250 1340.6354 1340.6361 -0.47
0
42
0.061
1
R.FYADNTVLDQR.L
795 707.4064 1412.7982 1412.7987 -0.33
0
54 0.0034
1
K.TQIATIGENLVVR.R
gi|171855138
Mass: 39114
Score: 131
Queries matched: 3
71.
gi|9858135
Mass: 46904
Score: 121
Queries matched: 4
Query Observed
Mr(expt)
Mr(calc)
50 396.2161
790.4176
790.4185 -1.04
0
34
0.45
1
481 567.8126 1133.6106 1133.6115 -0.71
0
30
1.4
1
1154 888.4882 1774.9618 1774.9618
0.05
0
35
0.24
1
1381 717.0550 2148.1432 2148.1442 -0.46
0
23
4.3
1
Peptide
K.LSVETSR.W
K.NTVSVTCLVK.D
R.VPQVYVLAPHPDELAK.N
K.CPAPELPGGPSVFIFPPKPK.D
Mass: 35862
Score: 121
Queries matched: 4
gi|18996195
72.
gi|157163930
Mass: 45716
Score:
Query Observed
Mr(expt)
Mr(calc)
271 489.7536
977.4926
977.4930
578 606.7800 1211.5454 1211.5458
666 635.8451 1269.6756 1269.6757
120
Queries matched: 3
concisus 13826]
-0.40
0
43
0.087
1
-0.32
0
36
0.23
1
-0.07
0
41
0.075
1
73.
gi|18996197
Mass: 35917
Query
Observed
Mr(expt)
481
567.8126 1133.6106
1156
889.4297 1776.8448
1358 1056.9910 2111.9674
74.
gi|157164029
Mass: 49918
Score: 116
Queries matched: 4
Query Observed
Mr(expt)
Mr(calc)
328 508.3019 1014.5892 1014.5896 -0.33
0
37
0.12
1
383 531.3138 1060.6130 1060.6128
0.21
0
22
3
1
474 565.3193 1128.6240 1128.6251 -0.94
1
27
2
1
866 736.4042 1470.7938 1470.7930
0.58
0
31
0.81
1
75.
gi|77736341
Mass: 52315
Query Observed
Mr(expt)
253 481.7488
961.4830
506 577.8356 1153.6566
680 641.3091 1280.6036
Peptide
K.NVDLSGFAR.Y
R.NEWSLDASYK.Y
K.LTFLSWYAAAK.D
Score: 119
Queries matched: 4
emPAI: 0.19
Mr(calc)
1133.6115 -0.71
0
30
1.4
1
K.NTVSVTCLVK.D
1776.8465 -0.91
0
29
1.6
1
2111.9688 -0.62
0
60 0.00074
1
K.YSTTPAQLDGDGSYFLYSK.L 1357
Peptide
R.GGIMQGLVIK.K.LSVVNISTTK.T
K.RGDLVISANGK.A
K.ISPDTQGVLVTDVK.S
Score: 115
Queries matched: 3
emPAI: 0.06
Mr(calc)
961.4828
0.21
0
61 0.0011
1
K.SSELVSANR.L
1153.6567 -0.08
1
24
2.4
1
K.SRLPGIVAEGR.S
1280.6037 -0.05
0
30
2.8
1
R.SDLYVSDAFHK.A
gi|109940161
Mass: 52314
Score: 115
gi|157838185
Mass: 48618
Score: 115
Queries matched: 3
Flags: Precursor
Queries matched: 3
76.
gi|27806941
Mass: 46075
Score: 112
Queries matched: 3
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
4 350.7336
699.4526
699.4531 -0.59
0
28
0.78
1
K.IVELVK.V
322 506.7746 1011.5346 1011.5349 -0.25
0
40
0.048
1
K.GNTHTEILK.G
976 797.4095 1592.8044 1592.8046 -0.11
0
43
0.055
1
K.SVLGDVGITEVFSDR.A
77.
gi|6320157
Mass: 19051
Functional homolog of human
Query Observed
Mr(expt)
7 353.7160
705.4174
612 617.8557 1233.6968
1262 641.3748 1921.1026
Score: 109
Queries matched: 3
emPAI: 0.38
NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention
Mr(calc)
705.4174
0.13
0
29
1.3
1
K.YVVVAR.A
1233.6969 -0.03
0
52 0.0036
1
K.IVEIPGEVPPGK.Y
1921.1037 -0.58
1
28
0.19
1
12/9/2009
Page 17 of 20
gi|151941952
Mass: 19021
Score: 109
Queries matched: 3
gi|190405057
Mass: 19007
Score: 109
Queries matched: 3
78.
gi|157165623
Mass: 66820
Score: 109
Queries matched: 3
emPAI: 0.10
aspartyl-tRNA synthetase [Campylobacter concisus 13826]
Query Observed
Mr(expt)
Mr(calc)
543 591.8032 1181.5918 1181.5928 -0.80
0
24
2.9
1
R.SSNEIFSSIAK.D
659 633.8240 1265.6334 1265.6326
0.70
0
53 0.0069
1
K.MIDVIDIFER.S
962 792.8814 1583.7482 1583.7468
0.95
0
32
0.64
1
K.FFEQSDLDEIVSR.C
79.
gi|157164353
Mass: 66046
Score: 107
Queries matched: 2
emPAI: 0.05
Query Observed
Mr(expt)
Mr(calc)
487 569.3166 1136.6186 1136.6190 -0.29
0
38
0.1
1
K.SLVIATGGYTR.I
1069 844.4465 1686.8784 1686.8788 -0.24
0
69 0.00017
1
R.LGGNSLTDAVVTGDLAGK.G
80.
gi|114051856
Mass: 51546
Score: 103
Queries matched: 2
emPAI: 0.06
Expect Rank Peptide
Query Observed
Mr(expt)
Mr(calc)
ppm Miss Score
423 547.2667 1092.5188 1092.5199 -1.00
0
28
1.1
1
K.AQYEEIANR.S
702 651.8611 1301.7076 1301.7078 -0.14
0
75 4.1e-005
1
R.SLDLDSIIAEVK.A
gi|116004057
Mass: 59000
Score: 103
Queries matched: 2
gi|149714762
Mass: 62397
Score: 103
Queries matched: 2
PREDICTED: similar to keratin 5 isoform 1 [Equus caballus]
81.
gi|154175347
Mass: 66396
Score: 102
Queries matched: 4
GTP-binding protein TypA/BipA [Campylobacter curvus 525.92]
Query Observed
Mr(expt)
Mr(calc)
264 487.7689
973.5232
973.5233 -0.01
0
21
8.5
1
364 525.2809 1048.5472 1048.5474 -0.18
0
26
1.9
1
408 544.3107 1086.6068 1086.6073 -0.46
0
32
1.1
1
1268 970.4792 1938.9438 1938.9436
0.15
0
22
7.2
1
Peptide
R.IEFEIPAR.G
K.TTMVDELLK.Q
R.GVLFLDPQAK.V
K.INIIDTPGHADFGGEVER.V
gi|157165061
Mass: 66311
Score: 102
Queries matched: 4
GTP-binding protein TypA/BipA [Campylobacter concisus 13826]
82.
gi|157164740
Mass: 36430
Score: 99
Queries matched: 2
emPAI: 0.19
Query Observed
Mr(expt)
Mr(calc)
707 653.3557 1304.6968 1304.6976 -0.58
0
56 0.0029
1
K.AVAEVLAGYGVEK.A
1077 845.9723 1689.9300 1689.9301 -0.04
0
44
0.026
1
83.
gi|157165180
Mass: 20941
Score: 89
Queries matched: 3
emPAI: 0.16
Query Observed
Mr(expt)
Mr(calc)
111 424.7657
847.5168
847.5167
0.15
0
21
5.5
1
R.IISVAFAK.T
1120 863.9703 1725.9260 1725.9261 -0.03
0
68 0.00013
1
R.LLPTNAVEILQTSGDR.A 1121
84.
gi|157164144
Mass: 33246
Score: 89
Queries matched: 2
emPAI: 0.10
Query
Observed
Mr(expt)
Mr(calc)
877
744.3960 1486.7774 1486.7780 -0.36
1
38
0.18
1
K.GLFVAGDIREDAPK.Q
1343 1039.4860 2076.9574 2076.9600 -1.22
0
50 0.0087
1
85.
gi|149699076
Mass: 61446
Score: 85
Queries matched: 3
PREDICTED: similar to carboxylesterase D1 isoform 1 [Equus caballus]
Query Observed
Mr(expt)
Mr(calc)
348 513.8007 1025.5868 1025.5869 -0.08
0
29
1
1
485 568.7648 1135.5150 1135.5146
0.43
0
29
1.2
1
705 653.3123 1304.6100 1304.6095
0.39
0
27
1.6
1
Peptide
K.IGIPTQAAQK.L
K.AVEEPPQTEH.K.EGASEEEINLSK.I
gi|504
Bovine
Query
149
425
1185
Peptide
K.DSALGFLR.I
K.YYGYTGAFR.C
86.
Mass: 75134
Score: 83
Lactoferrin [Bos taurus]
Observed
Mr(expt)
Mr(calc)
439.7400
877.4654
877.4658
549.2554 1096.4962 1096.4978
914.4410 1826.8674 1826.8686
Queries matched: 3
-0.35
0
37
0.34
4
-1.40
0
21
5.2
1
-0.65
0
24
4.5
1
Mass: 78070
Score: 83
Queries matched: 3
gi|506
12/9/2009
Page 18 of 20
gi|163285
Mass: 77873
Score: 83
Queries matched: 3
lactoferrin
gi|408928
Mass: 78117
Score: 83
Queries matched: 3
lactoferrin
gi|9650974
Mass: 78025
Score: 83
Queries matched: 3
gi|30794292
Mass: 78006
Score: 83
Queries matched: 3
Mass: 78129
Score: 83
Queries matched: 3
gi|146157606
Mass: 76095
Score: 83
Queries matched: 3
gi|157830374
gi|193299659
Mass: 78006
Score: 83
Queries matched: 3
gi|255762013
Mass: 76226
Score: 83
Queries matched: 3
87.
gi|157164277
Mass: 36126
Score: 76
Queries matched: 3
emPAI: 0.09
Query Observed
Mr(expt)
Mr(calc)
746 678.8781 1355.7416 1355.7409
0.58
0
25
2.6
1
R.AAALNIGPTTTGAAK.A 745
1042 828.4736 1654.9326 1654.9328 -0.08
0
51 0.0025
1
gi|255323513
Mass: 36026
Score: 76
Queries matched: 3
glyceraldehyde-3-phosphate dehydrogenase, type I [Campylobacter showae RM3277]
88.
89.
gi|157165403
Mass: 22036
Score: 73
Queries matched: 2
Query Observed
Mr(expt)
Mr(calc)
459 559.3337 1116.6528 1116.6543 -1.28
0
28
0.79
1
619 622.8458 1243.6770 1243.6772 -0.12
0
46
0.029
1
Peptide
K.FLGALLQVEK.K
R.AGSGEVGINLLSK.F
gi|225632
Mass: 24420
casein alphaS1
Query Observed
Mr(expt)
661 634.3557 1266.6968
776 692.8688 1383.7230
Peptide
R.YLGYLEQLLR.L
R.FFVAPFPEVFGK.E
Score: 72
Mr(calc)
1266.6972
1383.7227
Queries matched: 2
emPAI: 0.29
-0.27
0
39
0.092
1
0.23
0
32
0.62
1
gi|30794348
Mass: 24513
Score: 72
Queries matched: 2
gi|70905149
Mass: 24383
Score: 72
Queries matched: 2
Mass: 24311
Score: 72
Queries matched: 2
gi|75038951
gi|159793187
Mass: 13839
Score: 72
Queries matched: 2
gi|159793191
Mass: 23598
Score: 72
Queries matched: 2
Mass: 18671
Score: 72
Queries matched: 2
gi|159793193
gi|159793197
Mass: 23473
Score: 72
Queries matched: 2
Mass: 22442
Score: 72
Queries matched: 2
gi|159793209
Mass: 24427
Score: 72
Queries matched: 2
gi|195973860
90.
gi|157165527
Mass: 43876
Score: 67
Queries matched: 2
205 467.2267 932.4388 932.4392 -0.37
0
24
5.5
1
271 489.7536 977.4926 977.4930 -0.40
0
43
0.087
1
Peptide
R.FAYQYNK.K
K.NVDLSGFAR.Y
91.
gi|32267367
Mass: 37749
Score: 66
Queries matched: 3
Query Observed
Mr(expt)
Mr(calc)
41 385.1974
768.3802
768.3806 -0.50
0
33
0.1
1
K.AADFFAK.R
805 711.9189 1421.8232 1421.8242 -0.66
1
33
0.12
1
K.KPIIVPEDAKGQK.M 804
92.
gi|28603766
Mass: 45268
Score: 65
Queries matched: 2
emPAI: 0.07
Query Observed
Mr(expt)
Mr(calc)
731 673.3680 1344.7214 1344.7224 -0.68
0
43
0.065
1
R.LPIQEAFLGAMR.T
1493 855.4240 2563.2502 2563.2514 -0.47
0
22
6.5
1
K.AQILEGLGIGPGEGSEEELHSASQR.L
93.
gi|157165093
Mass: 50503
Score: 63
Queries matched: 2
12/9/2009
Query
154
404
94.
Observed
440.7498
542.3235
Mr(expt)
879.4850
1082.6324
Mr(calc)
879.4854
1082.6335
gi|15020816
Mass: 37415
Query
Observed
Mr(expt)
769
689.8755 1377.7364
1394 1094.9880 2187.9614
-0.45
1
28
0.22
1
-0.98
0
36
0.099
1
Page 19 of 20
Peptide
K.FKFDVPK.N
K.LINEELKPK.F
Score: 62
Queries matched: 2
emPAI: 0.09
Mr(calc)
1377.7364
0.01
0
24
3.8
1
K.VNNQALPQPIER.T
2187.9597
0.82
0
38
0.076
1
K.YSTTQAQQDSDGSYFLYSK.L
gi|15026997
Mass: 37400
Score: 62
Queries matched: 2
gi|194228715
Mass: 56103
Score: 62
Queries matched: 2
PREDICTED: immunogobulin gamma 1 heavy chain constant region [Equus caballus]
95.
gi|157164830
Mass: 38482
Score: 59
Queries matched: 2
Query Observed
Mr(expt)
Mr(calc)
406 543.8054 1085.5962 1085.5968 -0.52
0
35
0.34
1
K.DLQIEELVK.L
630 624.3945 1246.7744 1246.7761 -1.34
0
24
0.12
1
K.VLHLLGGANLLK.V
96.
gi|157165286
Mass: 58599
Score: 57
Queries matched: 2
Query Observed
Mr(expt)
Mr(calc)
764 685.8511 1369.6876 1369.6878 -0.11
0
23
4.4
1
815 715.3453 1428.6760 1428.6772 -0.84
1
34
0.38
1
Peptide
K.GDTALFFGLSGTGK.T
R.YEDVKEGVEYAK.A
gi|157164930
Mass: 58243
Score: 53
Queries matched: 2
Query Observed
Mr(expt)
Mr(calc)
636 625.8530 1249.6914 1249.6918 -0.27
1
24
2.4
1
796 709.3877 1416.7608 1416.7612 -0.28
0
29
1.4
1
Peptide
K.SVEILGDYKVK.I
K.LNAPAISNYEVVK.S
97.
98.
gi|154175135
Mass: 70659
Score: 53
Queries matched: 1
emPAI: 0.05
heat shock protein 90 [Campylobacter curvus 525.92]
Query Observed
Mr(expt)
Mr(calc)
914 763.4144 1524.8142 1524.8148 -0.34
0
53
0.006
1
R.GVIDVEDLPLNVSR.E
gi|157164405
Mass: 70627
Score: 53
Queries matched: 1
heat shock protein 90 [Campylobacter concisus 13826]
99.
gi|157347169
unnamed protein
Query Observed
288 494.7948
Mass: 30214
Score: 52
Queries matched: 1
emPAI: 0.11
product [Vitis vinifera]
Mr(expt) Mr(calc)
987.5750 987.5753 -0.26
0
52 0.0081
1
K.LDVLYPIR.G
Mass: 29623
Score: 52
Queries matched: 1
gi|225450829
PREDICTED: hypothetical protein [Vitis vinifera]
100.
gi|149716548
Mass: 30312
Score: 52
Queries matched: 1
emPAI: 0.11
Query Observed
Mr(expt)
Mr(calc)
793 706.8560 1411.6974 1411.6983 -0.62
0
52 0.0089
1
R.EYVAQFEASALGK.Q
gi|184185461
Mass: 30485
Score: 52
Queries matched: 1
apolipoprotein A-I (predicted) [Rhinolophus ferrumequinum]
101.
gi|157165029
Mass: 34028
Score: 52
Queries matched: 1
emPAI: 0.10
Query Observed
Mr(expt)
Mr(calc)
692 644.8325 1287.6504 1287.6499
0.40
0
52 0.0084
1
K.AYFDVVYGLNK.K
102.
gi|160893406
Score: 51
Queries matched: 2
hypothetical protein CLOL250_00955 [Clostridium sp. L2-50]
4 350.7336 699.4526 699.4531 -0.59
0
28
0.78
1
5 350.7337 699.4528 699.4531 -0.31
0
26
1.4
1
103.
Peptide
K.IVELVK.T
R.VVIELK.K
gi|154175276
Score: 49
Queries matched: 1
malic enzyme NAD binding subunit [Campylobacter curvus 525.92]
Query Observed
Mr(expt)
Mr(calc)
968 793.9306 1585.8466 1585.8464
0.16
0
49
0.011
1
Peptide
K.ALAPTFGGINLEDIR.A
12/9/2009
Page 20 of 20
Mass: 44829
Score: 49
Queries matched: 1
gi|157165008
transketolase A [Campylobacter concisus 13826]
gi|223040740
Score: 49
Queries matched: 1
12/9/2009

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