MSTP Post Fall 2013 - University of Wisconsin School of Medicine

Fall 2013
THE MSTP POST
THE MSTP POST
MSTP Symposium 2014:
The Who What When and Where
Details You’ve Been Waiting For
The annual MSTP symposium has been scheduled for
Thursday, April 24th, 2014
and will be held in the Wisconsin Institutes for Discovery Town Center and H.F.
DeLuca Forum.
Our keynote speaker this
year will be Dr. Mike Yaffe
(pictured to the left). Dr.
Yaffe will be visiting from
Massachusetts Institute of
Technology, where he currently serves as the David
H. Koch Professor of Biology and Biological Engineering. He is also affiliated with the Beth Israel
Deaconess Medical Center of Harvard Medical
School where he practices as an attending surgeon
and has helped to found multiple pharmaceutical
companies.
Dr. Yaffe recieved his MD-PhD training at Case
Western Reserve University. His lab currently investigates signaling pathways that control cell cycle
progression and DNA damage responses in cancer
and cancer therapy as well as the cross-talk between
inflammation, cytokine signaling, and cancer.
In addition to Dr. Yaffe’s address, the event will also
showcase research from current MSTP students in
the form of posters and talks throughout the day. For
all those current MSTP students with awesome
research to share, there will be a call for student
presenters in Spring of 2014.
In the past, other disinguished guests in attendence
at MSTP Symposium have included UW MSTP
alumni, physician scientists currently affiliated with
UW-Madison, and former UW MSTP director Deane
Mosher. So mark your calendars because you won’t
want to miss it!
University of Wisconsin - Madison
In This Issue
A look at the M1 class
and our new program
directors
Page 2; Page 4
Scientific Review: Spotlight on Jeff Jensen
Page 3
New MSTP Publications
Pages 5-7
Scenes from the MSTP
Retreat 2013
Page 8
Cook’s Corner: Food
Edition
Page 9
A Day in the Life: Spotlight on Laura Felley
Page 10
On Wisconsin!
1
Fall 2013
THE MSTP POST
Wow, those are some really really ridiculously good looking people!
A quick look at our
2013 M1 MSTP class.
From left to right:
Richard Merkhofer
Jack Hunt
Erin DeCloux
Mazdak Bradberry
Yang Hu
Amelia Haj
Peter Carlson
Jorge Rodriguez-Gil
Nick Vogt
Laura Swanson
Check out Page 4 for some quick facts about our
new administrators and our M1 class!
We didn’t just gain a new M1 class this fall, we also welcomed two new associate
directors to the program. Here’s a quick look at these new members of the
UW MSTP community.
Caitlin Pepperell MD
Scott Reeder MD, PhD
Caitlin Pepperell recieved
her MD from Queen’s University in Kingston, ON.
She then continued her
medical training with an
internal medicine residency
and fellowship in infectious
Scott Reeder recieved his
MD-PhD from Johns Hopkins University in Baltimore,
MD and continued his training with a radiology residency and fellowship in
abdominal and cardiovascu-
disease at the University of Toronto and an
additional fellowship in infectious disease
at Stanford University.
Dr. Pepperell’s research focuses on bacterial pathogenesis with an emphasis on
granulomatous diseases, human-pathogen
interactions, and the ecology and evolution
of human pathogens.
lar imaging at Stanford University.
In addition to acting as an MSTP associate
director, he now serves as the UW Chief of
MRI and the Director of the UW Liver Imaging Research Program. Dr. Reeder’s current
research focuses on the development of new
MRI methods and contrast agents for quantitative measures used to assess healthy and
diseased livers.
Fun fact: Both of our new MSTP associate directors come to us from Canada, eh?
University of Wisconsin - Madison
2
THE MSTP POST
Fall 2013
Scientific Review: Jeff Jensen’s recent publication “MAP3K8 kinase regu-
lates myeloma growth by cell-autonomous and non-autonomous mechanisms
involving myeloma-associated monocytes/macrophages”
by Laura Felley
Multiple myeloma has been a thorn in
the side of oncologists for decades. The
production of neoplastic plasma cells
essentially chokes the bone marrow,
preventing the formation of normal blood
cells and causing anemia. Overproduction of light chains by the malignant cells
can damage the kidneys, resulting in
renal failure. Patients may complain of
chronic bone pain, due to lesions and
fractures precipitated by overactive
osteoclasts stimulated tumor cell RANKL
expression. However, the truly disheartening feature of this disease is the lack of
good treatment options: while many
oncologists use combinations of chemotherapy, radiotherapy, and immunomodulators, the disease remains incurable. Average survival for an untreated
patient is three to four years. Aggressive,
intensive therapy only boosts the mean
survival time to five to seven years. With
this bleak prognosis in mind, Jeff’s goal is
to identify new targets for therapy by
studying the immunologic milieu associated with the cancer.
Jensen’s lab noticed a strange feature of
the macrophages associated with myeloma tumors. Typically, a macrophage
is thought of as either M1 or M2. A macrophage with an “M1” profile represents
what we classically think of as a macrophage: these cells are very anti-microbial,
readily engulfing pathogens or infected
cells. M1 macrophages have a high IL-12
to IL-10 secretion ratio, which is typically
regarded as inflammatory. An “M2”
macrophage is referred to as “alternatively activated”, and can be viewed as
the anti-inflammatory counterpart to the
M1 cell. M2 macrophages are involved in
healing and inflammation resolution, and
secrete comparably high levels of IL-10.
Tumor associated macrophages are gen-
University of Wisconsin - Madison
erally thought of as having an M2 profile.
Jensen’s lab previously showed that macrophages located in myeloma tumors acquire
an inflammatory phenotype, and transcribe
genes associated with an M1 profile. However, the lab has also found that these myeloma macrophages/monocytes are not limited to M1-associated genes, and freely transcribe genes associated with an M2 profile.
This led the group to hypothesize that myeloma promotes “significant plasticity” in
terms of inflammatory profile. But how
might this plasticity be achieved?
A potential mechanism was identified in
MAP3K8. The beautifully named mitogenactivated protein kinase kinase kinase 8
appears to have a key role in regulating the
innate immune response, as well as growth
signals capable of activating ERK. (ERK,
which stands for “extracellular-signal-related
kinase”, is a component of an extensive
signaling pathway involved in mitosis and
meiosis). Jensen and his lab hypothesized
that MAP3K8 regulates macrophagemyeloma tumor cell interactions within the
e bone marrow; and it is through these intertions that macrophages achieve their promyeloma inflammatory phenotype.
To test this hypothesis, bone marrow aspirates were collected from patients with multiple myeloma. Macrophages were isolated
and lysed. An antibody against phosphorylated (and thus activated) MAP3K8 was
added to the lysates. Of all samples collected,
MAP3K8 was universally phosphorylated,
regardless of disease stage or treatment
received. Furthermore, phosphorylated ERK
was seen in myeloma patient smaples, but
not in control samples—this finding reflects
the fact that MAP3K8-dependent ERK activity promotes the synthesis of pro-myeloma
inflammatory mediators. Likewise, ADAM17, a protease needed to cleave and process
Continued on Page 4
3
Fall 2013
THE MSTP POST
The MSTP Post polled the incoming
M1 class and the MSTP Administrators with random questions.
Here ‘s what we learned*.
More M1’s prefer cake to pie. (The great
cake-pie divide was split evenly among
our administrators.)
Unnecessary Pie Chart
About M1 Pie Preferences
Prefer Pie
Prefer Cake
The M1 class is rather well-travelled!
Total Countries Visited
This is not the appropriate way to
graph the worldliness of our M1 class
20
15
10
5
0
0
2
4
6
8
10
M1 Responder
The high school versions of our administrators were more mathematically inclined
than the high school versions of our M1s.
Why is this format even an option?
0.8
0.6
0.4
0.2
0
Admininstrators
M1s
Proportion reporting a better score on SAT math than SAT verbal
One administrator reports their age as 100!
*Facts and figures based on responses from 9
M1’s and 4 MSTP Administrators
University of Wisconsin - Madison
Continued from Page 3
the pro-inflammatory TNFα, is a known target
of ERK. As one might expect, analysis of the
lysates revealed constitutive ADAM-17 phosphroylation at a site regulated by MAP3K8
signaling.
Interestingly, MAP3K8 activation seems to
be very important to multiple myeloma;
expression levels of activated MAP3K8 are
much higher as compared to other tumor cells.
To investigate whether MAP3K8 is capable of
transmitting inflammatory signals in myeloma
cells themselves, the lab used a line of human
myeloma tumors. It is known that myeloma
cells respond to the inflammatory TNFα signal
by activating ERK, but the lab found that this
effect could be reduced by the addition of a
MAP3K8 inhibitor. Inhibiting MAP3K8 also
reduces phosphorylation of another kinase
(MEK kinase); this reduction in kinase activation is associated with a deficit in growth.
Reasoning that MAP3K8 appears to be tightly
linked to myeloma growth, myeloma cells
were imaged for MAP3K8 activation as well as
cell cycle markers. Cells that contained activated MAP3K8 tended to show ploidy (an
increased number of chromosome sets). Interestingly, MAP3K8 was not only necessary for
expansion—it also appeared to be critical for
survival. Myeloma cells showed higher levels
of apoptosis when blocked with a MAP3K8
inhibitor, even when incubated with supportive cellular stroma.
It is clear that MAP3K8 appears essential to
multiple myeloma cells in numerous contexts.
--Its expression in tumor-associated macrophages promotes an inflammatory environment and growth signals that are beneficial to
myeloma survival, while its expression in the
cancerous cells promotes proliferation and
survival. MAP3K8 thus appears to be a fantastic therapeutic target. Amazingly, loss of
MAP3K8 is compatible with normal blood cell
growth and development, so a treatment that
inhibited the kinase would likely be welltolerated. Jeff’s lab is currently testing its
therapeutic potential in mouse models of myeloma, with promising responses. Go Jeff, and
great paper!
4
Fall 2013
THE MSTP POST
New Pubs!
Wow, the UW MSTP certainly seems to
have had a prolific year. Here are all the
publications our students have contributed to since the last newsletter, including those from our class of 2013 grads.
Good job, everyone!
Jabe Best:
Markandeya YS, Feng L, Best JM, Lea
ML, Buss CR, Kamp TJ, Balijepalli RC.
Loss of Caveolin-3 Results in Qt(C)
Prolongation and Causes Delayed
Cardiac Repolarization in a CardiacSpecific Conditional CAV-3 Knockout
Mouse Model. Biophysical Journal. 2013
Jan 29;104(2):209A-209A
Boczek NJ, Best JM, Tester DJ, Giudicessi
JR, Middha S, Evans JM, Kamp TJ,
Ackerman MJ. Exome sequencing and
systems biology converge to identify
novel mutations in the L-type calcium
channel, CACNA1C, linked to autosomal dominant long QT syndrome. Circ
Cardiovasc Genet. 2013 Jun;6(3):279-89.
PMID: 23677916
Patrick Brown:
Brown PT, Handorf AM, Jeon WB, Li
WJ. Stem Cell-based Tissue Engineering
Approaches for Musculoskeletal Regeneration. Curr Pharm Des. 2013 Feb
11;19(19):3429-45. PMID: 23432679
Eric Bultman:
Brodsky EK, Bultman EM, Johnson KM,
Horng DE, Schelman WR, Block WF,
Reeder SB. High-spatial and hightemporal resolution dynamic contrastenhanced perfusion imaging of the liver
r with time-resolved three-dimensiona
l radial MRI. Magn Reson Med. 2013 Ma
r 20. [Epub ahead of print] PMID
: 235198
Tim Chang:
Chang TS, Lemanske Jr RF, Guilbert TW,
Gern JE, Coen MH, Evans MD, Gangnon
RE, Page CD, Jackson DJ. Evaluation of
the modified asthma predictive index in
high risk preschool children. Journal of
Allergy and Clinical Immunology: In
Practice. 2013 Mar; 1(2):152-156.
Peter Chen:
Colson BA, Patel JR, Chen PP,
Bekyarova T, Abdalla MI, Tong CW,
Fitzsimons DP, Irving TC, Moss RL.
Myosin binding protein-C phosphorylation is the principal mediator of protein
kinase A effects on thick filament
structure in myocardium. J Mol Cell
Cardiol. 2012 Nov;53(5):609-16. PMID:
22850286
Jason Chiang:
Chiang J, Hynes K, Brace CL. Flowdependent vascular heat transfer during
microwave thermal ablation. Conf Proc
IEEE Eng Med Biol Soc. 012;2012:5582-5.
PMID: 2336719
Chiang J, Hynes KA, Bedoya M, Brace
CL. A dual-slot microwave antenna for
more spherical ablation zones: ex vivo
and in vivo validation. Radiology. 2013
Aug;268(2):382-9. PMID:23579048
Chiang J, Wang P, Brace CL. Computational modelling of microwave tumour
ablations. Int J Hyperthermia. 2013
Jun;29(4):308-17. PMID: 23738698
Omar Demerdash:
Zhu X, Ericksen SS, Demerdash ON,
Mitchell JC. Data driven models for
protein interaction and design. Proteins.
2013 Aug 28. [Epub ahead of print]
PMID: 24038640.
Mike Devinney:
Devinney MJ, Huxtable AG, Nichols NL,
Mitchell GS. Hypoxia-induced phrenic
long-term facilitation: emergent properties. Ann N Y Acad Sci. 2013
Apr;1279(1):143-53. PMID: 23531012
Mike Devinney & Daryl Fields:
Devinney MJ, Fields DP and Mitchell
GS. Spinal PKC-θ activity is required for
phrenic long-term facilitation after acute
intermittent hypoxia. Faseb Journal.
2013 Apr;27.
John Floberg:
Struck AF, Hall LT, Kusmirek JE,
Gallagher CL, Floberg JM, Jaskowiak CJ,
Perlman SB. (18)F-DOPA PET with and
without MRI fusion, a receiver operator
characteristics comparison. Am J Nucl
Med Mol Imaging. 2012 Oct 30;2(4):47582. PMID: 23145363
Floberg JM, Struck AF, Peters BK,
Jaskowiak CJ, Perlman SB, Hall LT.
Impact of expectation-maximization
reconstruction iterations on the diagnosis of temporal lobe epilepsy with PET.
Am J Nucl Med Mol Imaging.
2012;2(3):335-43.
Floberg JM, Holden JE. Nonlinear
spatio-temporal filtering of dynamic
PET data using a four-dimensional
Gaussian filter and expectationmaximization deconvolution. Phys Med
Biol. 2013 Feb 21;58(4):1151-68. PMID:
2337069
Brendan Floyd:
Still AJ, Floyd BJ, Hebert AS, Bingman
CA, Carson JJ, Gunderson DR, Dolan
BK, Grimsrud PA, Dittenhafer-Reed KE,
Stapleton DS, Keller MP, Westphall MS,
Denu JM, Attie AD, Coon JJ, Pagliarini
DJ. Quantification of mitochondrial
acetylation dynamics highlights prominent sites of metabolic regulation. J Biol
Chem. 2013 Sep 6;288(36):26209-19.
PMID: 23864654
University of Wisconsin - Madison
Travelle Franklin-Ford:
Franklin-Ford T, Shah N, Leiferman E,
Chamberlain CS, Raval A, Vanderby R,
Murphy WL. Tracking injectable microspheres in dynamic tissues with encapsulated superparamagnetic iron oxide
nanoparticles. Macromol Biosci. 2012
Dec;12(12):1615-21. PMID: 23124987
Keith Hanson:
Zhan L, Hanson KA, Kim SH, Tare A,
Tibbetts RS. Identification of Genetic
Modifiers of TDP-43 Neurotoxicity in
Drosophila. PLoS One. 2013;8(2):e57214.
PMID: 23468938
Shawn Jackson:
Jackson SS, Coughlin EE, Coon JJ,
Miyamoto S. Identifying posttranslational modifications of NEMO by
tandem mass spectrometry after high
affinity purification. Protein Expr Purif.
2013 Sep 6. [Epub ahead of print] PMID:
2401278
Jeffrey Jensen:
Hebron E, Hope C, Kim J, Jensen JL,
Flanagan C, Bhatia N, Maroulakou I,
Mitsiades C, Miyamoto S, Callander N,
Hematti P, Asimakopoulos F. MAP3K8
kinase regulates myeloma growth by
cell-autonomous and non-autonomous
mechanisms involving myelomaassociated monocytes/macrophages. Br J
Haematol. 2013 Mar;160(6):779-84.
PMID: 2325262
Asimakopoulos F, Kim J, Denu RA,
Hope C, Jensen JL, Ollar SJ, Hebron E,
Flanagan C, Callander N, Hematti P.
Macrophages in multiple myeloma:
emerging concepts and therapeutic
implications. Leuk Lymphoma. 2013 Apr
11. [Epub ahead of print] PMID:
23432691
*Co-authored by Ryan Denu
Bornali Kundu:
Kundu B, Sutterer DW, Emrich SM,
Postle BR. Strengthened effective
connectivity underlies transfer of
working memory training to tests of
short-term memory and attention. J
Neurosci. 2013 May 15;33(20):8705-15.
PMID: 23678114
Voss J, Meier TB, Freidel R, Kundu B,
Nair VA, Holdsworth R, Kuo JS, Prabhakaran V. The role of secondary motor
and language cortices in morbidity and
mortality: a retrospective functional MRI
study of surgical planning for patients
with intracranial tumors. Neurosurg
Focus. 2013 Apr;34(4):E7. PMID:
23544413
Continued on Page 6
5
Fall 2013
THE MSTP POST
Continued from Page 5
Kundu B, Penwarden A, Wood JM,
Gallagher TA, Andreoli MJ, Voss J,
Meier T, Nair VA, Kuo JS, Field AS,
Moritz C, Meyerand ME, Prabhakaran
V. Association of functional magnetic
resonance imaging indices with
postoperative language outcomes in
patients with primary brain tumors.
Neurosurg Focus. 2013 Apr;34(4):E6.
PMID: 23544412
Eric Landsness:
Plante DT, Landsness EC, Peterson MJ,
Goldstein MR, Riedner BA, Wanger T,
Guokas JJ, Tononi G, Benca RM.
Sex-related differences in sleep slow
wave activity in major depressive
disorder: a high-density EEG investigation. BMC Psychiatry. 2012 Sep
18;12:146. PMID: 22989072
Plante DT, Goldstein MR, Landsness
EC, Peterson MJ, Riedner BA,
Ferrarelli F, Wanger T, Guokas JJ,
Tononi G, Benca RM. Topographic and
sex-related differences in sleep
spindles in major depressive disorder:
a high-density EEG investigation. J
Affect Disord. 2013 Mar
20;146(1):120-5. PMID: 22974470
Plante DT, Goldstein MR, Landsness
EC, Riedner BA, Guokas JJ, Wanger T,
Tononi G, Benca RM. Altered
overnight modulation of spontaneous
waking EEG reflects altered sleep
homeostasis in major depressive
disorder: A high-density EEG investigation. J Affect Disord. 2013 Sep
25;150(3):1167-73. PMID: 23810359
Josh LaRocque:
LaRocque JJ, Lewis-Peacock JA,
Drysdale AT, Oberauer K, Postle BR.
Decoding attended information in
short-term memory: an EEG study. J
Cogn Neurosci. 2013 Jan;25(1):127-42.
PMID: 23198894
Emrich SM, Riggall AC, Larocque JJ,
Postle BR. Distributed patterns of
activity in sensory cortex reflect the
precision of multiple items maintained
in visual short-term memory. J
Neurosci. 2013 Apr 10;33(15):6516-23.
PMID: 23575849
Siclari F, Larocque JJ, Postle BR,
Tononi G. Assessing sleep consciousness within subjects using a serial
awakening paradigm. Front Psychol.
2013 Aug 20;4:542. PMID: 23970876
Jeremy Lavine:
Krautkramer KA, Linnemann AK,
Fontaine DA, Whillock AL, Harris TW,
Schleis GJ, Truchan NA, Marty-Santos
L, Lavine JA, Cleaver O, Kimple ME,
Davis DB. Tcf19 is a novel islet factor
necessary for proliferation and
survival in the INS-1 beta cell line. Am
J Physiol Endocrinol Metab. 2013
Sep;305(5):E600-10. PMID: 23860123
*Co-authored by Kim Krautkramer
Rachel Lenhart:
Thelen DG, Lenz AL, Francis C, Lenhart
RL, Hernández A. Empirical assessment
of dynamic hamstring function during
human walking. J Biomech. 2013 Apr
26;46(7):1255-61. PMID: 23540723
Francis CA, Lenz AL, Lenhart RL,
Thelen DG. The modulation of forward
propulsion, vertical support, and center
of pressure by the plantarflexors during
human walking. Gait Posture. 2013 Jun
17. pii: S0966-6362(13)00239-7. [epub
ahead of print] PMID: 23787149
Lenhart RL, Thelen DG, Wille CM,
Chumanov ES, Heiderscheit BC. Increasing Running Step Rate Reduces Patellofemoral Joint Forces. Med Sci Sports
Exerc. 2013 Aug 2. [Epub ahead of print]
PMID: 23917470
Mike Levine:
Levine MN, Hoang TT, Raines RT.
Fluorogenic probe for constitutive
cellular endocytosis. Chem Biol. 2013
Apr 18;20(4):614-8. PMID: 23601650
David Manthei:
Denlinger LC, Manthei DM, Seibold
MA, Ahn K, Bleecker E, Boushey HA,
Calhoun WJ, Castro M, Chinchili VM,
Fahy JV, Hawkins GA, Icitovic N, Israel
E, Jarjour NN, King T, Kraft M, Lazarus
SC, Lehman E, Martin RJ, Meyers DA,
Peters SP, Sheerar D, Shi L, Sutherland
ER, Szefler SJ, Wechsler ME, Sorkness
CA, Lemanske RF Jr; the NHLBI Asthma
Clinical Research Network Investigators.
P2X 7 –Regulated Protection from
Exacerbations and Loss of Control is
Independent of Asthma Maintenance
Therapy. Am J Respir Crit Care Med.
2013 Jan 1;187(1):28-33. PMID: 23144325
Anna Mirer:
Hagen EW, Mirer AG, Palta M, Peppard
PE. The sleep-time cost of parenting:
sleep duration and sleepiness among
employed parents in the wisconsin sleep
cohort study. Am J Epidemiol. 2013 Mar
1;177(5):394-401. PMID: 23378502
Clint Morgan:
Morgan CT, Noble D, Kimble J. Mitosismeiosis and sperm-oocyte fate decisions
are separable regulatory events. Proc
Natl Acad Sci U S A. 2013 Feb
26;110(9):3411-6. PMID: 2340150
Julian Motzkin:
Pujara M, Motzkin JC, Newman JP,
Kiehl KA, Koenigs M. Neural correlates
of reward and loss sensitivity in
psychopathy. Soc Cogn Affect Neurosci.
2013 May 9. [Epub ahead of print]
PMID: 23552079
University of Wisconsin - Madison
Farsh Moussavi-Harami:
Moussavi-Harami SF, Annis DS, Ma W,
Berry SM, Coughlin EE, Strotman LN,
Maurer LM, Westphall MS, Coon JJ,
Mosher DF, Beebe DJ. Characterization of
Molecules Binding to the 70K N-Terminal
Region of Fibronectin by IFAST Purification Coupled with Mass Spectrometry. J
Proteome Res. 2013 Jul 5;12(7):3393-404.
PMID: 23750785
*Co-authored by MSTP 2012 graduate Lisa
Maurer
Philip Mudd:
Mudd PA, Martins MA, Ericsen AJ, Tully
DC, Power KA, Bean AT, Piaskowski SM,
Duan L, Seese A, Gladden AD, Weisgrau
KL, Furlott JR, Kim YI, Veloso de Santana
MG, Rakasz E, Iii SC, Wilson NA, Bonaldo
MC, Galler R, Allison DB, Piatak Jr M,
Haase AT, Lifson JD, Allen TM, Watkins
DI. Vaccine-induced CD8(+) T cells control
AIDS virus replication. Nature. 2012 Nov
1;491(7422):129-33. PMID: 23023123
Walsh AD, Bimber BN, Das A, Piaskowski
SM, Rakasz EG, Bean AT, Mudd PA,
Ericsen AJ, Wilson NA, Hughes AL,
O'Connor DH, Maness NJ. Acute Phase
CD8+ T Lymphocytes against Alternate
Reading Frame Epitopes Select for Rapid
Viral Escape during SIV Infection. PLoS
One. 2013 May 6;8(5):e61383. PMID:
23671565
Mike Palte:
Caes BR, Palte MJ, Raines RT. Organocatalytic conversion of cellulose into a
platform chemical. Chemical Science. 2013
April; 4(1):196-199.
Arick Park:
Yang C, Park AC, Davis NA, Russell JD,
Kim B, Brand DD, Lawrence MJ, Ge Y,
Westphall MS, Coon JJ, Greenspan DS.
Comprehensive Mass Spectrometric
Mapping of the Hydroxylated Amino
Acid residues of the α1(V) Collagen Chain.
J Biol Chem. 2012 Nov 23;287(48):40598610. PMID: 23060441
Kelli Pointer:
Pointer K, Kuo JS, Dempsey RJ.
CLARITY-A Clearer View of the Brain.
Neurosurgery. 2013 Aug;73(2):N16. PMID:
23867270
Rene Roy:
Roy RM, Paes HC, Nanjappa SG, Sorkness
R, Gasper D, Sterkel A, Wüthrich M, Klein
BS. Complement component 3C3 and C3a
receptor are required in chitin-dependent
allergic sensitization to Aspergillus
fumigatus but dispensable in chitininduced innate allergic inflammation.
MBio. 2013 Apr 2;4(2). PMID: 2354991
Roy RM, Klein BS. Fungal glycan interactions with epithelial cells in allergic airway
disease. Curr Opin Microbiol. 2013
Aug;16(4):404-8. PMID: 23602359
Continued on Page 7
6
Fall 2013
THE MSTP POST
Continued from Page 6
Brandhorst TT, Roy R, Wüthrich M,
Nanjappa S, Filutowicz H, Galles K,
Tonelli M, McCaslin DR, Satyshur K,
Klein B. Structure and function of a
fungal adhesion that binds heparin and
mimics thrombospondin-1 by blocking T
cell activation and effector function.
PLoS Pathog. 2013 Jul;9(7):e1003464.
PMID: 23853587
Yash Somnay:
Somnay Y, Simon K, Harrison AD,
Kunnimalaiyaan S, Chen H, Kunnimalaiyaan M. Neuroendocrine phenotype
alteration and growth suppression
through apoptosis by MK-2206, an
allosteric inhibitor of AKT, in carcinoid
cell lines in vitro. Anticancer Drugs. 2013
Jan;24(1):66-72. PMID: 23147412
Somnay Y, Chen H, Kunnimalaiyaan M.
Synergistic Effect of Pasireotide and
Teriflunomide in Carcinoids in vitro.
Neuroendocrinology. 2013;97(2):183-92.
PMID: 22965070
Aadhavi Sridharan:
Sridharan A, Xu G, Kastman E, Oh J,
Ries M, Hermann B, LaRue A, Asthana
S, Carlsson C, Gleason C, Sager M,
Johnson S. Longitudinal changes in left
anterior hippocampal activation in
cognitively normal subjects with APOE
4 polymorphism. Alzheimer’s &
Dementia. 2011 Jul;7(4):S225-S225.
Sridharan A, Pehar M, Shahriar Salamat
M, Pugh TD, Bendlin BB, Willette AA,
Anderson RM, Kemnitz JW, Colman RJ,
Weindruch RH, Puglielli L, Johnson SC.
Calorie restriction attenuates astrogliosis
but not amyloid plaque load in aged
rhesus macaques: a preliminary quantitative imaging study. Brain Res. 2013
May 1;1508:1-8. PMID: 23473840
Jon Stefely:
Hebert AS, Merrill AE, Stefely JA, Bailey
DJ, Wenger CD, Westphall MS, Pagliarini DJ, Coon JJ. Amine-reactive
neutron-encoded labels for highlyplexed proteomic quantitation. Mol Cell
Proteomics. 2013 Jul 23. [Epub ahead of
print] PMID: 2388203
Jeremy Weiss:
Weiss JC, Natarajan S, Peissig PL,
McCarty C, Page D. Machine Learning
for Personalized Medicine: Predicting
Primary Myocardial Infarction from
Electronic Health Records. AI Magazine.
2012 Dec;33(4):33-45.
Weiss J, Natarajan S, Page D. Multiplicative Forests for Continuous-Time
Processes. Neural Information Processing Systems (NIPS). 2012 Dec; 25;467475.
Weiss JC, Natarajan S, Peissig P,
McCarty C, Page CD. Statistical
Relational Learning to Predict Primary
Myocardial Infarction from Electronic
Health Records. 2012 July. Proceedings
of the Twenty-Fourth IAAI Conference.
Weiss JC, Page CD. Forest-based point
processes for event prediction from
electronic health records. In Blockeel H,
Kersting K, Nijssen S, Zelezny F (eds.),
Machine Learning and Knowledge and
Discovery in Databases. Berlin,
Germany: Springer Berlin Heidelberg.
2013: 547-562).
Andrew Wentland:
Kramer JH, Arnoldi E, François CJ,
Wentland AL, Nikolaou K, Wintersperger BJ, Grist TM. Dynamic and
Static Magnetic Resonance Angiography
of the Supra-aortic Vessels at 3.0 T:
Intraindividual Comparison of Gadobutrol, Gadobenate
Dimeglumine, and Gadoterate Meglumine at Equimolar Dose. Invest Radiol.
2013 Mar;48(3):121-8. PMID: 23211552.
Richard Yang:
Van De Voort TJ, Felder MA, Yang RK,
Sondel PM, Rakhmilevich AL. Intratumoral delivery of low doses of anti-CD40
mAb combined with monophosphoryl
lipid a induces local and systemic
antitumor effects in immunocompetent
and T cell-deficient mice. J Immunother.
2013 Jan;36(1):29-40. PMID: 23211623
Yang RK, Kalogriopoulos NA, Rakhmilevich AL, Ranheim EA, Seo S, Kim
K,Alderson KL, Gan J, Reisfeld RA,
Gillies SD, Hank JA, Sondel PM. Intratumoral treatment of smaller mouse
neuroblastoma tumors with a recombinant protein consisting of IL-2 linked to
the Hu14.18 antibody increases intratumoral CD8+ T and NK cells and
improves survival. Cancer Immunol
Immunother. 2013 Aug;62(8):1303-13.
PMID: 23661160
Brittany Young:
Gallagher TA, Nair VA, Regner MF,
Young BM, Radtke A, Pankratz J,
Holdsworth RL, Baniulis D, Kornder
NK, Voss J, Austin BP, Moritz C,
Meyerand ME, Prabhakaran V. Characterizing the relationship between
functional MRI-derived measures and
clinical outcomes in patients with
vascular lesions. Neurosurg Focus. 2013
Apr;34(4):E8 . PMID: 23544414
Wentland AL, Wieben O, François CJ,
Boncyk C, Munoz Del Rio A, Johnson
KM, Grist TM, Frydrychowicz A. Aortic
pulse wave velocity measurements with
undersampled 4D flow-sensitive MRI:
comparison with 2D and algorithm
determination. J Magn Reson Imaging.
2012 Nov 2. PMID: 23124585
Wentland AL, Grist TM, Wieben O.
Repeatability and Internal Consistency
of Abdominal 2D and 4D Phase Contrast
MR Flow Measurements. Acad Radiol.
2013 Jun; 20(6):699-704. PMID: 23510798
Nadia Sundlass:
Sundlass NK, Eller CH, Cui Q, Raines
RT. Contribution of Electrostatics to the
Binding of Pancreatic-Type Ribonucleases to Membranes. Biochemistry. 2013
Aug 30. [Epub ahead of print] PMID:
23947917
Sarah Wernimont:
Wiemer AJ, Wernimont SA, Cung TD,
Bennin DA, Beggs HE, Huttenlocher A.
The focal adhesion kinase inhibitor
PF-562,271 impairs primary CD4+ T cell
activation. Biochem Pharmacol. 2013 Sep
15;86(6):770-81. PMID: 23928188
Heather Taff:
Mitchell KF, Taff HT, Cuevas MA,
Reinicke EL, Sanchez H, Andes DR. Role
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University of Wisconsin - Madison
In case you missed the
emails, Grumpy Cat Drew
wants you to know that the
UW MSTP has a blog.
Check out
wisconsinmstp.blogspot.com
for more Drew and other
related media from the UW
MSTP! Updated daily.
Send in blog submissions to
[email protected]
7
Fall 2013
THE MSTP POST
Photos!
Snapshots from the
2013 UW MSTP Retreat
Ray Zhang,
Jason Chiang,
and Andy
Voter enjoying
the last days of
summer
University of Wisconsin - Madison
8
Fall 2013
THE MSTP POST
Cook’s Corner
In this issue’s Cook’s Corner, students wanted to hear
Paul’s take on food, food, and more food. In particular, we wanted to get the scoop on some of his most
popular recipes.
Would you share the recipe for your Ginger Salsa?
See Side Panel
Would you share the recipe for your "salmony dip"?
Dip for salmon:
Mayonaise and ground horseradish to taste...... that's
it, really!
Some of us have heard about The Church of Paul,
also known as Brasserie V. When is the most likely
time that you might be found at your church, and
more importantly, what should someone order
when dining there?
The Church of Paul and All Mankind, otherwise
known as the Brasserie V on Monroe Street, has many
fine dishes. You may find me there at lunch or after
work, but I have been so busy that I haven't worshiped for some time. If you need a tour or companion, let me know. The Moules and Frites (mussels
and french fries) are a favorite..... however, I have
burned out on them. If you are there at lunch, the
sandwiches (the Ahi Tuna and Avacado BLT are two
of my favorites) and soups are wonderful. Saint
Bernardus Apt 12 is my beer of choice. Enjoy!
And a short announcement from Paul Cook himself:
I have officially moved!
My new office is next to Chelsea's, 2375 HSLC. It is
deep in the library, 2nd Floor. If you haven't been to
Chelsea's office yet, here are the directions. Enter the
library and turn left, walk to the end of the space and
turn right. Straight ahead you will see a door with a
sign that reads "Information Resources, Information
Architecture, Rm 2370-2376." Go through that door
(it is not a trap) and turn to your right. My office is
straight ahead. I lovingly call it "Storeroom B."
Come visit sometime!
University of Wisconsin - Madison
PAUL’S SALSA
1 lg. can diced tomatoes – well
drained, then finely chopped and
drained again
1 garlic clove – minced
Equal amount of fresh ginger
root – minced
4-5 scallions, with green portion
– chopped
Fresh cilantro – chopped
Fresh basil – chopped (dried
basil is OK, too)
1-2 jalapeno peppers, with seeds
– chopped
Salt and pepper - to taste
Squeeze of lime juice
(optional....if it needs a little zest)
This is fairly easy. I am careful
to drain the tomatoes as well as
possible... I don’t want the salsa
to be too watery. (The lime juice
adds more liquid.) All ingredients are adjustable to your taste.
The ginger root and garlic are
essential, the fresh herbs are a
real treat.
Enjoy!!
9
Fall 2013
THE MSTP POST
A day in the life...
...of a 2nd year clinical investigations PhD student
Compiled by Cecilia Westbrook and Laura Felley
Name: Laura Felley
Year: G2
PhD Program: Clinical Investigations (ICTR)
Lab and general focus: Gumperz Lab, NKT cell
Research interests: I’m interested in determining
how the application of NKT cells might be used to
modulate immune responses to viral infections.
A project you're working on: Right now, I’m looking
at inflammasome activation in NKT-instructed
monocytes. The inflammasome is a protein complex
that activates caspase-1, which cleaves all sorts of
stuff, including the pro-form of IL-1β, so we use the
production of mature IL-1β as an indicator of inflammasome activation.
5:00 AM: Stagger out of bed to feed the cats. Plan on
changing into gym clothes to go for an early morning
run. But internet is calling me…
5:30 AM: Stare blankly at eyebrows in bathroom
mirror. Blink at self. Look at gym clothes on the
floor.
5:50 AM: Finally out the door!
7:00 AM: Stagger back inside. Be grateful that the
weather has cooled, thus staunching the epic torrents
of sweat. Wonder how some girls manage to look
attractive while exercising.
7-8 AM: Shower, dress, and assemble ALL THE
FOODS: three breakfasts (banana “pancake” for me,
bagel for Doug*, severed nightcrawler for axolotols),
1 lunch (ritz crackers and peanut butter, apple), 1
dinner (Trader Joe’s Breakfast Burrito, for I am a
rebel).
8-8:30 AM: I’m never sure what happens in this extra
half hour that ensures I’m never out the door by 8,
but it’s always there.
9:00 AM: Arrive in lab with Doug, who actually
works 2 floors upstairs as the Forrest’s lab artist in
residence. Draw blood for today’s experiment.
9-10 AM: As blood separates, work on a clinical
trial protocol proposal for BMI 544 (despite the fact
that I do no clinical research forever) and a piece in
the MSTPost.
10-11:30 AM: Isolate PBMCs, harvest and count
NKT cells, plan out experiment. Realize I left the
Trader Joe’s Breakfast Burrito on the counter.
Wonder how long I’d have to nuke the burrito to
kill the bacteria.
11:30 AM: Lab meeting is supposed to start.
11:50 AM: Lab meeting actually starts. Newish
post-doc is presenting data from his PhD. It looks
better than mine. I am jealous.
12:45 PM: Sneak out of lab meeting early to go to
Stat 641.
1-2:15 PM: Stat 641 is an actual statistics class with
math and everything, so I am woefully unprepared. Thankfully, the professor is competent,
kind and non-judgmental (useful when I show up
to office hours).
2:30-4:15 PM: The free-for-all. Finish setting up
first half of an experiment (I’m generating supernatants for a calcium flux assay that I will do tomorrow morning, which will require yet more blood),
answer emails, finish paper, eat stale leftover mini
donut from lab meeting, am unsatisfied with
donut, buy peanut butter M&Ms and tea and eat
those too.
4:40-6:10 PM: BMI 544. It is long. The students are
all early- to mid-career doctors, so I feel young and
stupid.
6:10-7:00 PM: Walk home
7:00-7:30PM: Futz around on internet, give up on
Trader Joe’s burrito, eat leftover tomato soup and a
grilled cheese instead.
7:30-9:00 PM: Study for virology exam on Friday,
interspersed with more futzing around online.
9:00-10:00 PM: Watch QI with Doug on Youtube
while half-heartedly pedaling on exercise bike.
10:15 PM: Goodnight
*Doug is Laura’s husband.
THE MSTP POST
THE MSTP POST
Editors:
Brittany Young
Sumit Kar
Many thanks to Chelsea Hanewall for making
the newsletter possible!
N.B. The MSTPost is written, edited, and assembled by
students of the UW MSTP. The views represented in the
MSTPost do not necessarily represent those of the University
of Wisconsin Madison, the UW School of Medicine and Public
Health, or the Medical Scientist Training Program.
University of Wisconsin - Madison
10