Billion Microbes
Transcription
Billion Microbes
BILLION MICROBES INTERNATIONAL SDN BHD (Company No: 1078763 W) BillionMicrobes No.15, Jalan PJU 7/23, Mutiara Damansara, 46000 Petaling Jaya, Selangor, Malaysia. Tel: (603) 7728 3122 Fax: (603) 7728 4122 E-mail : [email protected] CONTENTS 1. BILLION MICROBES (PRODUCT DETAIL) 3-19 2. MATERIAL SAFETY DATA SHEET & TEST REPORT 20-26 3. DOSAGE / APPLICATION 27-29 4. TESTIMONIES (BILLION MICROBES) 30-42 5. LAPORAN - TEST PLOT MADA REPORT 43-54 6. LAPORAN - TEST PLOT BERNAS REPORT 55-67 7. COST & PROFIT 68 8. MULTIPLE APPLICATION OF BILLIONMICROBES 69-76 9. NURSERIES 77-84 10. COMPOSTING 85-108 11. BILLION MICROBES – MISSION 109 12. ARTICLE ON MICROBES BY ANN REID (Director of the American Academy of Microbiology) 110-117 13. BACKGROUND OF THE COMPANY 118-120 2 BILLION MICROBES (PRODUCT DETAILS) 3 What Does Billion Microbes Do? “Billion Microbes” is a product that protects healthy plants, promotes their growth and restores beneficial soil Microbes. 1. Protection The product contains Thricoderma that works in a natural way to protect plant roots from soil pathogen attack such as Pythium, Fusarium, Scleroctinia and Rhizoctonia or generally described as Fungi. 2. Growth Promoter Healthy plants will have more roots to assimilate nutrients from the soil. In this case, it is nitrates (NO3-) that most plants need to grow. By adding BillionMicrobes to the soil, it will help boost microbial activities and convert Nitrogen (N2) to Nitrates (NO3-). 3. Soil Remediation Spraying BillionMicrobes to the soil not only helps keep plants in good condition. It also introduces the product micro organisms to the soil environment. In a normal situation, fertilizers, chemical herbicides and pesticides are used to provide nutrients to control unwanted weeds and steer pests away from the plants. At the same time, the leftover by-products are transformed into damaging pollutants to the soil making it unsuitable for microbes to live in. By introducing our product to the soil, it helps add and replace the lost chain in the Nitrogen cycle thus making the soil fertilized with healthy nutrients. BillionMicrobes has been tested on paddy fields, palm tree plantations, cash crop, landscape area, plant nurseries and even in household flowerpots and vases. The results were overwhelmingly positive and the cost of maintaining the soil was greatly reduced. 4 At the same time, the leftover by-products are transformed into damaging pollutants to the soil making it unsuitable for microbes to live in. By introducing our product to the soil, it helps add and replace the lost chain in the Nitrogen cycle thus making the soil fertilized with healthy nutrients. BillionMicrobes has been tested on paddy fields, palm tree plantations, cash crop, landscape area, plant nurseries and even in household flowerpots and vases. The results were overwhelmingly positive and the cost of maintaining the soil was greatly reduced. 5 Palm Tree Plantations 6 Oil Palm Harvesting & Extraction 7 Statistics of Palm Oil in Malaysia & Indonesia 8 Characteristic of Oil Palm 9 Characteristic of Oil Palm 10 Paddy Planting 11 Paddy Grains 12 Indonesia 2012 Paddy Rice Production Up 5% at 69 Million Tons: Statistics Agency Indonesia’s 2012 paddy production is estimated to have reached about 69.05 million tons (about 46.4 million tons, basis milled), up about 5% from the previous year, according to the country’s Central Statistics Agency (BPS). The BPS said that the increase in rice production last year is due to increase in rice acreage to around 13.4 million hectares (6.2 million hectares in Java, and 7.2 million hectares in other islands), up about 1.8% or 239,800 hectares from 2011. Yield also increased by about 0.15 tons per hectare to around 5.9 tons per hectare, said the agency. The BPS uses a milling conversion rate of 62.7%, almost on par with that of the USDA. However, the USDA estimates of around 57.48 million tons of paddy (about 36.5 million tons, basis milled) production figures for 2012 are about 17% lower than the estimates by the BPS. USDA estimates of rice acreage at around 12.1 million hectares and yield of about 4.7 tons per hectare in 2012 are also lower than those of the BPS. Final estimates by the BPS are expected in July. Indonesia is targeting self-sufficiency in rice this year. The country’s rice buying agency, Bureau of logistics (BULOG), has said that it would not import any rice this year if the government production target of around 72 million tons of paddy (a year-on-year increase of around 3% as per PBS estimates) is met in 2013. In a report published earlier this year, the USDA suggested that Indonesia may achieve self-sufficiency in rice in 2013, and most of the 800,000 tons of rice imports scheduled in 2013 would comprise 2012 carry over imports by BULOG. 13 Indonesia rice production 2013 Indonesia 2013 Paddy Rice Production Forecast to Reach 69.3 Million Tons Indonesia’s paddy rice production is expected to reach around 69.3 million tons (about 43.6 million tons, basis milled) in 2013, down from previous forecasts due to localized adverse weather... 4 months 1 week ago Indonesia rice production 2013 , Asia, Indonesia _______________________________________________________________________ Indonesia Rice Self-Sufficiency Likely to Continue in Coming Years, Says Minister Indonesia is expected to sustain its rice self-sufficiency in the remaining months of this year and in the coming years due to higher production and increasing government stocks, the deputy agriculture... 4 months 2 weeks ago Indonesia rice imports, Indonesia rice production 2013 , Asia, Indonesia ________________________________________________________________________ USDA Post Projects Indonesia MY 2012-13 Rice Imports at 1 Million Tons The USDA Post in Jakarta says that Indonesia’s total rice exports in MY 2012-13 (beginning January 2013) may reach about one million tons, down about 49% from an estimated 1.96 million tons of rice... 6 months 3 weeks ago Indonesia rice imports, Indonesia rice production 2013 , Asia, Indonesia ________________________________________________________________________ Indonesia Close to Self-Sufficiency in Rice Government initiatives to increase rice acreage and favorable weather conditions are likely to help rice production in Indonesia cross the targeted 72 million tons of paddy (about 48 million tons, basis... Indonesia rice production 2013, FAO, USDA, Central Statistics Agency, BPS 14 Kiraan untuk Keluasan tanah dalam ukuran Hektar, Ekar dan Relung 1 hektar = 2.471054 ekar 1 hektar = 3.4749196 relung persegi 1 hektar = 10 km persegi 1 ekar = 4046.86 meter persegi (4.046.86 km persegi) 1 ekar = 0.4047 hektar 1 ekar = 1.40625 relung persegi 1 relung = 2,877.764 meter persegi (2.877.764 km persegi) 1 relung =0.28777 hektar 1 relung = 0.71111111 ekar 15 The Anatomy of Rice 16 Billion Microbes 1. Liquid form biological treatment product. 2. Combination of Micro Organisms with nutrients and stimulants. 3. Promote healthier plant growth. Enhancing soil structure. 17 Billion Microbes AGRO is a synergistic blend of spore-forming microbes in liquid form, which decomposes organic materials in the root zone mix, and restricts pathogenic diseases through its aggressive enhancement of supplying vital proteins, vitamins, and growth regulators to the plants. ADVANTAGES o o o o o Improves root zone and its ability to receive adequate moisture for healthy growth and improved yield. Improves decomposition of toxic chemicals Increases availability of vital nutrients (i.e. nitrogen and phosphorus) to the roots Helps prevent disease Reduces the need for harsh chemicals, fertilizer, and fungicide use The 5 “E” BENEFITS 1. EFFECTIVE 2. EASY 3. ECONOMICAL 4. ECO-FRIENDLY 5. ELSEWHERE 1. EFFECTIVE • Increases yields up to 30% or more on poor soils • Will provide a maximum increase of yield up to 50% compared with non-microbe yields on normal soil 2. EASY • To apply, just spray on soil or foliage • Does not require additional machinery or manpower • Mix in with pesticides during routine spraying i.e. can piggy-back on existing chemicals and spraying schedule. (Hence, not a separate spraying and less costly / fussy procedures) 3. ECONOMICAL • Eventually after 6 months, will replace up to 30% of current fertilizer usage thus resulting in big savings. • Provides a firewall protection against spread of Ganoderma and Xanthomonas, long term prophylactic protection against new infection of some fungi and bacterial organisms within the plant tissues. • Proven also against Bacterial Leaf Blight, Red Rust, Moko, Panama disease etc. 4. ECO-FRIENDLY • Does not easily leach into rivers (Rhizosphere colonizing – sticks to roots) • Increases soil hydration hence less dependence on irrigation. (Trehalose effect) • Promotes sustainability. Less Ganoderma infestation to reused / reploughed Palm Oil 2nd generation plantations. Hence, chances are less need to clear new jungle areas for crop. Acceptability to Eurozone sustainability practices. • Revitalizes / replenishes ‘dead’ soil within six (6) months. 5. ELSEWHERE (OTHER CROPS / APPLICATION) • Rice (Paddy) • Oil Palm • Maize • Most Legumes • Vegetables • Wheat • Sugarcane • Rye • Barley • Tea • Fruits • Alfalfa / Rapeseed • Sorghum and etc. 18 Key Features • Nitrogen fixing bacterium • Phosphate solubizing bacterium • Potash solubizing and bacteria • Trichoderma Fungus • Seaweed Extract • Humid Acid APPLICATION: Once monthly for first six (6) months and thereafter once every two (2) months. Dilute 1 liter to 40 parts of water/mix with fertilisers and/or pesticides/herbicides and spray directly on the soil. BIO-CONTROL ADVANTAGES : • Non pathogenic to Oil Palm and environment. Root / Rhizosphere preference hence not leached away or diluted during heavy rains/ flooding. • Able to compete with other pathogens and persist in soil (Rhizosphere competent.) • Lengthy shelf life. Resistant to most commercial fertilizers, pesticides and fungicides. Attachment to the host hyphae by coiling : Lectin-carbohydrate interaction Penetrate the host cell walls by secreting lytic enzymes : a. Chitinases b. Proteases c. Glucanases Successfully prevents the spread of Ganoderma by : •Direct competition for space and nutrients. •Producing toxins against Ganoderma. •Induces the host plant to secrete its own antibiotics for localized control of pathogens •Also protects roots from other diseases e.g. Pythium, Rhizoctonia and Fusarium 19 MATERIAL SAFETY DATA SHEET & TEST REPORT BY JABATAN PERTANIAN (DEPARTMENT of AGRICULTURE) BAHAGIAN PEGURUSAN DAN PEMULIHARAAN SUMBER TANAH 20 21 22 23 24 25 How Safe Is BillionMicrobes? 1.This is a liquid form biological treatment product. It is an all-natural product, safe for the environment and living beings. Selected Class One (1) micro organisms have been combined with nutrients and stimulants, resulting in a higher potential of achieving the expected results. The concentrated product does not include any harmful substance making it easy to handle and use. This product is developed and produced specifically to promote healthier plant growth by enhancing the soil structure. 2.BillionMicrobes contains selective strains of Class One (1) natural occurring micro organisms, cultured under stringent quality control using a unique formula suitable for tropical climates. The ingredients also include living spores of selected fungi in the genus Trichoderma that will colonize plant roots making them impenetrable to other fungi that may cause disease to plants. 26 DOSAGE / APPLICATIONS 27 28 Application of Billion Microbes to Paddy Plantation Standard End User Price Computation (2014 only) 1 Acre of Paddy Field requires ½ Liter of Microbes Per Spray 5 Sprays (five (5) times) Per season of 95-120 days on 1 Acre = 5 x ½ Liters per Spray = 2.5 Liters of Spraying per acre per season recommended on day 1, 20, 45, 65, 85 RM120.00 per liter x 2.5 Liters of Spray = RM300.00 per Acre per Season of 5 Sprays 29 TESTIMONIES (BILLION MICROBES APPLICATIONS) 30 OUR CASE HISTORY A farmer in Rawang & Ulu Yam, Selangor was trying to maximize his yield for cucumbers and was struggling with weak plants with leaves that yellow prematurely and fruits that are mal-formed and drop prematurely. After consulting with Billion Microbes International Sdn Bhd Specialists, we recommended a dual regime of spray whereby the 2.3 acres of land was treated with 2 liters of BillionMicrobes and the seedlings for the cucumber was sprayed with 10ml of BillionMicrobes after appropriate dilution. We followed the growth of the plants from inception to fruit bearing and it is captured below in the following chronograph. Week 1: Just finished tilling the soil and spraying the farm with BillionMicrobes after appropriate dilution Sprayed Sapling in Week 3 after soil and bed preparation 31 Week 7 28 days after planting of Seedlings. Plant has grown rapidly with lush leaves that are larger than normal and fruit is already forming on the vines 32 Week 8 The plants show no sign of fading after the first week of fruiting and is continuing to grow robustly and forming a canopy along the wires. In fact many of the trees are also producing multiple fruits. 33 As can be seen from the pictures, the case study for cucumber was highly successful in a farm where some of the crops has failed. This time around, the farmer has experienced a bumper crop not only in the number of cucumbers but also the size and weight of the cucumbers. The flesh to seed ratio is also very large making for juice cucumbers. The cucumbers regularly weigh from 600 to 700 gram. After the successful application BillionMicrobes the farmer will be using it on all his other produces to enhance the soil conditions and restore it to its natural fertile state as well as to improve plant health and yields. 34 Fruits slightly more than 8 inches in length with a width of a Business Card. 35 Miraculously, a “Nam-nam” Tree that was barren for 7 years started flowering within two weeks of receiving treatment of Billion Microbes and subsequently bore its first fruit upon receiving a treatment of Billion Microbes (Soil Microbes). "A Revolutionary Formula Break Through." 36 37 Comparison Between Palm Oil Fruits With Microbes Application And Non-Microbes Application Fruit sprayed with Billion Microbes on the right hand side appears bigger in size with thicker flesh causing the “kernel” to be small resulting in production of more oil. 38 39 40 41 Comparison Between Palm Oil Fruits With Microbes Application And Non-Microbes Application Fruit sprayed with Billion Microbes appears bigger in size with thicker flesh Mesocarp resulting in production of more oil. (OER = 37%) Concurrently the kernel size is reduced to 30% Paddy Plants sprayed with Billion Microbes have increased number of productive tillers, 25% to 30% taller plants, longer leaves and more upright, fresh green leaves with no burnt tips. Proven extra yield between 25% - 69.4% for comparison done in various season.1000 grain weight for MR220 is 36.5 grams. Application of Billion Microbes to crops at the Nursery level right from the commencement of planting up to the bearing of th e fruits Billion Microbes promotes healthier, fresher and greener vegetation enhancing faster growth and larger size of plants and fruits. The size of the banana is bigger 42 It is visibly evident after the application of Billion Microbes the fruits become larger and leaves become bigger as seen above. Laporan(Report) Laporan (Report) Test Plots MADA BB-IV Sg Sg.. Korok Korok,, Alor Setar Setar,, Kedah, Malaysia 43 44 44 45 45 46 46 47 48 49 50 51 52 53 54 Laporan(Report) Laporan (Report) Test On Projek Plot Sawah Padi Bernas Kg. Kebun Teduh Teduh,, Kedah, Malaysia 55 56 57 58 59 60 61 62 Summary of Results Padi Test Plot En Ismail Sharii • Increased in yield for En Ismail test plot as shown in Bernas report dated 24th Mac 2013 is 23% • However, this does not account for several important factors namely:- 1. Fertilisation was only done once this time. However, it should have been 4 times. There was no insecticide and fungicide application. 2. 50% or 0.72 hectares of the plot was affected seriously by Weedy rice which reduced considerably the final harvest caterpillar and fungal problems arose in late season. 3. Despite these problems there was still and overall yield increased of 0.9 metric tons in the 1.4 hectare trial plot compared to the similar season in the previous year. 63 64 65 66 HAJI ISMAIL SHARII’S* PADDY YIELD COMPARISON FOR 8 SEASONS Berikut adalah hasil padi Hj Ismail sepanjang 8 musim berturut-turut iaitu:- Musim • 1/2014 • 2/2013 Berat Bersih (kg) 11,204.00 11,778.00 NEW Average: 11,491 kg • • • • • • 4,848.00 9,354.00 3,821.00 3,586.00 9,312.60 9,786.00 1/2013 2/2012 1/2012 2/2011 1/2011 2/2010 +69.4% increased in yield Average: 6,784.60 kg Untuk dapatkan berat kasar, bahagikan berat bersih dengan 83%. Ini adalah kerana purata potongan pemutuan di kawasan utara adalah 17%. Contoh kiraan berat kasar:11,204kg / 83% = 13,498.80kg. Berat Kasar = 13,500kg (Jumlah berat kasar perlulah dalam kiraan 10 terdekat ) 67 COST AND PROFIT PLOT OWNER : HAJI ISMAIL SHARII PLOT ADDRESS : KG. KUBANG TEDUH, SUNGAI BARU , KEDAH 1000 Grain Weight (mixed sample) Average Number of Grains per Panicle = 35.7 grams = 67 grains. Total number of Tillers = 2571 Number of Failed Tillers per av. m² sample = 1.18 Number of Productive Tillers per m² sample = 512 Av. Number of Tillers per m² = 514 % Failure Rate of Tillers = 0.92 Total Grain Weight for m² sample = 1.360 kg Extrapolated Total Grain Weight (include Failed Grains) For 1 Hectare (10,000 m²) This Season = 13.60 MT/ Hectare % Moisture = 14% Official Results Report for 5 Relong Season: May – Sept 2012 Total Yield Weight = 11,778 kg = 2.36 MT/Relong ( No Microbes Applied) Season: May – Sept 2013 Total Yield Weight = 14,202 kg = 2.84 MT/ Relong (Application of Billion Microbes) Yield increase due to Application of Billion Microbes +480kg per Relong = + 1680kg per Hectare = + RM 2,352.00 per Hectare Amount spent on Billion Microbes / Relong = RM 200.00 per season Five (5) Applications per season per Hectare = RM 750.00 per season Return of Investment = RM 2,352.00 / RM 750.00 # Increased Yield of 3.1 times in One (1) Season 68 BILLION MICROBES (MULTIPLE APPLICATIONS) 69 Pemilik Sawah Padi : En. Lee Sui Ann Sekinchan, Selangor Gambar menunjukkan penyakit Bacterial Leaf Blight (BLB) secara dekat pada hari ke-56 dan seterusnya jangkitan merebak ke daun utama (flag leave) pada hari ke-68 Gambar menunjukkan serangan Bacterial Leaf Blight (BLB) pada hari ke-60 dengan tiada tanda-tanda menunjukkan penyakit ini akan terus reda 70 Tapak ujian jenis padi MR 220 pada hari ke-68 yang disembur dengan BillionMicrobes ini dijangkiti Bacterial Leaf Blight (BLB) pada hari ke-50 Selepas semburan BillionMicrobes serangan BLB menurun dan terkawal dalam masa 5 hari Tapak padi hari ke-60 tidak menunjukkan ciri-ciri serangan BLB seperti mana pada hari ke-48 ini setelah Jadual semburan BillionMicrobes telah dilaksanakan 71 Pemilik Sawah Padi: En. Samat Md Nafiah Tali Air 7, Sg Leman, Sekinchan, Selangor. Kemunculan daun utama (flag leaves) tangkai padi tidak terbantut 72 Pemilik sawah Padi: Dato’ Badaruddin Jalil Mohamad Azmi Lot 24, Pengkalan Kundur, Jalan Sg Korok, Alor Setar, Kedah No BillionMicrobes Thrip infestation Day 20 Final yield 1.23mt/relong Water stress Day 75-90 BillionMicrobes No Thrip infestation Final yield 2.01 mt/relong Moderate resistance to water stress Vegetative growth 25% 73 l Fruits slightly more than 8 inches in length with a width of a Business Card. Size Banana bigger than the Blackberry Handphone 10 month old Samples upon delivery to UTCL (EPA Management Sdn Bhd) on 23rd of November 2012 Application of BillionMicrobes Oil Palm Non Application of BillionMicrobes Oil Palm 74 Non Application of BillionMicrobes Application of BillionMicrobes SABAH TEA PLANTATION TRIAL PLOT WITH APPLICATION OF BILLIONMICROBES Before (18 April 2012) 45 Days after Spraying (After 15 June 2012) Usage of Application: One (1) Liter for Day 1 and one (1) Liter for Day 30 Total of 2 Applications 75 TEST PADI PLOT AT KEDAH Non Application of BillionMicrobes Paddy Field Application of BillionMicrobes Paddy Field Application of BillionMicrobes Close up Image Application of BillionMicrobes Close up Image 76 NURSERIES 77 78 10 month old Samples upon delivery to UTCL (EPA Management Sdn Bhd) on 23rd of November 2012 Billion Microbes WORKER 5’ 6” ht CONTROL 79 UTCL Biomass measurements & preparation 80 Refer to full set report Cohort - Billion Microbes 4 samples Control – 4 samples 3 months pre-nursery, 6 months nursery Plants transferred to plantation in late November 2012 Standard MPOB fertiliser regime All summarized comments assumed referring to Billion Microbes compared to Control Samples SOIL ANALYSIS Less acidic 5.125 : 4.93 +6% total nitrogen +5% organic carbon Carbon to nitrogen ratios marginally less -28% total phosphorus Available phosphorus almost equal Exchangeable K & Mg -20% Ca +16% Na, SO4 same Cation exchange capacity +9% 81 K -10% in stem Mg -25% in roots N marginally higher in leaves marginally lower in roots and stems P same in both Ca -25% in all leaf, stem and roots Boron +10% in all leaf, stem and roots Fe (ferum) -40% in leaves, -6% in roots Cu -20% in roots, +3% in leaf and stem Mn roots -12% in leaf, -12% in stem, + 5% in Zn notably lower in all leaf, stem and roots especially in roots where it is -60% Total ash content % is same at around 24% There is +30% more derived from roots 82 1.Total wet weights; Control = 4300g Billion Microbes = 6550g + 52% more in Billion Microbes 2.Total dry weights: Control = 1550g Billion Microbes = 1925g + 24% more in Billion Microbes 3. Percentage loss of mass due to lab dessication; Control = 65% loss Billion Microbes = 71% loss +9% more hydrated in Billion Microbes 4. Wet root mass expressed as a % of the total plant biomass; Control = 19.7 % Billion Microbes = 22.7 % +12 % more in wet root percentages 83 PLANT PHYSICAL ANALYSIS CONTROL Billion Microbes Pinne Lengths Microbes 43.26cm vs 52.51cm +21.4% Billion Pinne Breaths Microbes 3.32 cm vs 3.60 cm +8% Billion # of Pinne Microbes 28.3pcs vs 35.8pcs +25% Billion # of Fronds Microbes 15.25pcs vs 16.75pcs +16% Billion Length of Fronds 144.5cm Microbes vs 191.25cm +32.4% Billion Highest Frond Microbes vs 216.5cm +23% Billion Girth Microbes 176cm 9.53 cm vs 11.58cm +21.5% Billion - 4.2% Control Petiole Weight 2.58 g vs 2.47 g Petiole Depth 1.5 mm vs 1.33mm -11.3% Control 84 COMPOSTING 85 A. Proposed use of Billion Microbes (BM) on shredded Empty Fruit Bunches in the Mill Tangible Benefits: 1. 2. 3. 4. Reduces cost of disposing EFB Savings from EFB transporting and application Expected sustainable yield increase (about 10% to 20%) with improved soil properties Expected revenue from additional yield increase within 9 months of application together with selected Billion Microbes Physical benefits 1. 2. 3. 4. 5. 6. Reduces input of chemical fertilizers leading to savings on fertilizer cost Increase nutrient uptake due to improvement of soil physically, chemically and biologically Reduces nutrient losses through surface runoff and leaching Conserves moisture Controls weed and soil erosion Environmentally friendly, reduces soil damage due to heavy application of chemical fertilizers 86 Early stage of aggressive Microbial degradation of fibres forming white fungal mat all over the surfaces. Later stage of Microbial degradation. The fibres had been broken down and became black in colour. Shredded Empty Fruit Bunches. Ingredients mixing with a Caterpillar shovel, which is also used in turning of compost piles in the aeration process. 87 EFB mobile-shredder in action. Compost piles in windrows covered with plastics to keep excess moisture out and maintain high temperature composting process. Compost dryer to dry the finished product to desirable moisture content. Finally, the finished product. 88 89 90 30 Days of deoiling of EFB compost result in a decrease of 97% from 0.14% to 0.03% oil content 91 30 Days of deoiling of EFB compost result in a decrease of 97% from 0.14% to 0.03% oil content 92 EFB COMPOST REPORT EFB Compost values 3 months after commencement of trial. Total Dry NPK = 9.68 C: N Ratio = 19.5 93 94 Simplified Flow Chart of Billion Microbes International Sdn Bhd Compost Technology for Palm Oil Mills Shredded EFB Essential Microbe Culture Oil Mill Wastes Wastes Mixing Windrows: Turning Shredding Temp. & pH monitoring Matured Compost Sieving (optional) Drying (optional) Applied in Plantation 95 Early stage of aggressive microbial degradation of fibres forming white fungal mat all over the surfaces Later stage of microbial degradation. The fibres had been broken down and became black in colour Shredded empty fruit bunches 96 Ingredients mixing with a Caterpillar shovel, which is also used in turning of compost piles in the aeration process EFB mobile-shredder in action The compost piles in windrows covered with plastics to keep excess moisture out and maintain high temperature composting process 97 Compost dryer to dry the finished product to desirable moisture content The finished product 98 B. PROPOSED USE OF BILLION MICROBES (BM) ON RAW UNSHREDDED EMPTY FRUIT BUNCHES IN THE OIL PALM PLANTATION Location : Any designated Prime Mature / Mature Oil Plantation Preliminary: i. Existing Palm Oil Mill with a limited special platform space Hence, no effective in-Mill Composting of EFBs ii. Current EFB output averaging 4,000 Metric Ton per month or more iii Storage and/or disposal is a pressing issue regards to space obstruction to smooth Mill operations and DOE’s guidelines and regulations iv. Provides for a slow release format of nutrients into the plantation Proposal Framework: • Placement of EFBs as inter-row rafts in Oil Palm Plantation within close proximity to the Mill • Arrangement as indicated below • Inter row Blanket arrangement width/length of heap = 1.8m x 9m • Height of Raft = 0.2m • Raft capacity (weight) = 3.2 m³ • Weight of Raft = 1mt or 1000kg approximately • One raft accessed by 4 Palms (average) • One Hectare = 136 Trees hence 34 rafts • It is an Open-Raft. No covering required • Estimated 30-35 Metric Ton of EFBs per hectare per application per year (Author: Loong et al.1998) 99 COMPARISONS BETWEEN MILL STANDARD COMPOSTING AND IN THE FIELD EFB COMPOSTING – DRAFT ONLY Mill Standard Compost Start up with shredded EFB Higher concentration Billion Lower activator requirements In-The-Field EFB Compost Mostly unshredded EFB Lower concentration Billion microbes Higher activator requirements Sprayed on site at Mill Treatment at Mill prior to transfer to field or spray at field location The former is more effective + economical Careful monitoring and periodic turning No monitoring required at field Temperature control No temperature control 2 month duration of composting-technically complete when temperature is below 58º Celsius Estimated 4-8 months composting. Temperature remains below 58º Celsius Ambient temperature at heap cortex Release of nutrients to soil over 2 months period (month 3+4) Release of nutrients to soil over 5 months period or longer Aesthetically compost finer with fibres available 1-2 inches long and separated (Day 60). Clumpy appearance with remnants of bunch maybe recognizable (Day 120). Some core polyphenols present. Fibres more than 4 inches and some dehiscence Cost of 1mt compost = RM 172 approximately Cost of 1mt treated EFB = RM 28 approx or cost of 1mt eventual compost is RM 56 Labour + machinery requirements. Controlled processing. Less labour No further machinery required after initial treatment. NPK attributes within a narrow range Total NPK above 6.5 NPK attributes within a broader range due to variable decomposition pattern and exposure to the elements. Application to field up to 6 times per year Application to field up to once a year All 34mt EFB to one hectare More likely to produce vegetative/fruiting improvements within 6 months of application based on a dosage per tree Will require more than 6 months to produce results based on a application rate UNLESS there is increase application weightage and active Billion microbes blanket spraying to the field at least once every 2 months No special obstruction at mill compound provided raw EFBs are treated The compost mill will require space to park 60 windrows total volume and transported to plantation promptly equivalent to 2 month’s production of EFBs at any one time Price per tree depends on dosage of compost per year, excluding packaging, labour and transport Price per tree RM7.00 per year exclude labour + transport NB: The above comparison chart and the prior proposal re use of Billion Microbes on EFB in the field are strictly draft copies and subject to amendments after site (Mill) evaluation and discussion with client. 100 101 102 103 104 PALM OIL TREES AS SOURCE OF RENEWABLE BIOMASS • Estimated 90 million tonnes of renewable biomass from Malaysia’s 3 million hectares of oil palm plantations and 300 palm oil mills. • Trunk, fronds and wastes (EFB’s, mesocarp fibre, shell, etc) are valuable Biomass. • EFB generated is about 12 millions tonnes and 25 million tonnes POME. Dept of Environment consider EFBs and POME as wastes, and impose stringent standards and regulations on its disposal. • By applying Billion Microbes and the right composting processes and technologies, these wastes could be recycled into high quality compost to enhance soil fertility, promote plant growth, increase yield, and reduce dependence on chemical fertilisers. 105 Our Consultancy and Management Services 1. Undertake Feasibility studies 2. Select Wastes for Composting • Empty fruit bunches • Mesocarp fibres • Kernel cake • Kernel shell • Decanter cake • Bunch ash • Pome 3. Technology Transfer • • Billion Microbes will provide on-site hands-on training on the composting for a period of 8 to 10 weeks until the first windrow is ready for use as compost. Excluding of operating cost, the production cost for one metric ton of compost ranges from RM180 and upwards. 106 Dr. Chew Boon Hock, KMN, AMN ; B. Sc. Hons (Mal.), M. Sc.(Wales), Ph.D. (Wales) He worked 30 years as Senior Scientist with MARDI (Malaysian Agricultural Research and Development Institute) in agricultural research, heading Rice Research and later Plant Science and Genetics Units. His expertise is in Organic Agriculture and has many years experience in Microbial Culture and Organic Composting Technology. Presently he is a Consultant in Organic Agriculture for Billion Microbes International Sdn Bhd. Dr. Frank Ow Yang Abdullah MBBCh, LRCP & SI, BAO, NUI. Director of Billion Microbes International Sdn Bhd. He obtained his Medical degree from the Royal College of Surgeons in Ireland. After working in the surgical field for 5 years, he started his landscaping company with interest in creative innovation. He applies his ideas to organic agriculture and has interest in beneficial Microbes. 107 CONCLUSION We humbly propose:•Commercial application of Billion Microbes Solution and Compost for Eco Harmonic Solutions Sdn Bhd and its market. 108 BILLION MICROBES MISSION Our Targets: 1. Rice Industry -Increased yield in the Paddy Plantations and control of certain diseases. 2. 3. Oil Palm Industry - Oil Palm Nurseries and Plantations - Increased yield - Oil Palm Mills for Composting of EFBs. - Increase resistance to Ganoderma. Animal Feedstock - As an important supplement to improve protein / nutrient availability. - Primary growth promoter for Napier Grass, Feedstock grains etc. 4. Sugar Industry - Increased yield in Sugar Plantations. 5. Fruit Industry - Increased yield in Banana Plantations. - Inhibits “Panama” disease and eliminates “Mako”. - Increase in the number, size and weight of fruits. 6. Vegetable Industry - Increased yield. 7. Tea Industry - Among others helps fight “Red Rust” Disease. - Increase the size of tea leaves thus enhancing the weight. 8. Aquaculture Tilapia (Water treatment) - Healthier fish and increase in size. 109 110 Microbes Helping To Improve Crop Productivity Plant-associated microbes not only provide several agronomic benefits but also furnish promising antimicrobial mixtures Ann Reid is the Director of the American Academy of Microbiology. Summary • Fungi associated with plant roots can increase the efficiency of phosphate uptake in crops such as potatoes and rice. • Plant-associated bacteria that produce a particular deaminase can protect host plants against a variety of stresses. • Plants carrying trehalose-producing bacteria prove resistant to drought and produce more foliage and deeper roots. • A double-stranded RNA virus, paired with a fungal endophyte, enables some plants to grow in high- temperature soils. • Some endophytic fungi produce mixtures of volatile chemicals with potent antimicrobial activity. 111 Nearly 1 billion people go hungry every day, and providing food for them is one of the great challenges facing humanity, a challenge that continues to grow. Arable land and water for irrigation are limited resources, while the productivity gains from the Green Revolution are now mostly part of the status quo. Moreover, the financial and environmental costs of using fossil fuels to ship foods and fertilizers around the world are becoming prohibitive. Plant breeding and engineering crop plants with newer genetic technologies continue to improve yields or other traits, but are expensive, slow, and applicable mainly to the most widely planted crop species. Further, genetically modifying each and every agriculturally valuable plant species to grow optimally in many different environments does not appear practical. Thus, we need less costly, more sustainable approaches to improving productivity of a wide variety of plant crops. One promising but largely untapped approach to improving crop plant productivity involves harnessing fungi and other plant-associated microorganisms, according to experts who spoke during a plenary session, "How Microbes Can Help Feed the World," convened during the 2011 ASM General Meeting in New Orleans last May. Those five scientists bring a different perspective to this challenge, one that considers how, over evolutionary time, plants and microbes developed mutually beneficial, cooperative relationships. Importantly, such microbe-plant partnerships can improve the resistance of host plants to a wide variety of stresses, including disease, drought, salinity, nutrient shortages, and extreme temperature. Further, understanding these natural relationships between host plants and their associated microorganisms could be put to better use and might lead to ways of increasing crop productivity while holding costs down and without harming the environment. Indeed, this approach could spark a new Green Revolution. 112 Several Critical Food-Crop Challenges Although humans need to produce more food, simply adding more and more fertilizers to current crops will do little to improve yields. Part of the global challenge of producing more food entails increasing the productivity of regionally important crops instead of merely boosting productivity of commodity crops being grown in already highly efficient settings. By relying more on locally produced foodstuffs, we can begin to move away from transporting commodities such as wheat flour and rice from one place to another while consuming more and more fossil fuels in the process. Another goal is to reduce the dependence of farmers, particularly those in developing countries, on imported seeds that, typically, are selected for their high productivity where they were bred. Greater agricultural productivity is unlikely to come from farmers expanding their efforts into new territories--the global supply of high quality arable land is more or less fixed, and unlikely to expand significantly without sacrifices, for example, in biodiversity. If anything, the goal is to grow crops on reduced acreage or on land that is considered marginally useful for agricultural purposes. Not all such land is marginal in the same way; some of it may be too dry, too salty, or have limited nutrients. Each situation calls for crop plants with different adaptations. Thus, we need to develop crop plants that continue to be productive even when growth conditions are poor. Rising global temperatures are yet another important factor complicating efforts to improve global crop productivity. One goal is to ensure that staple crops continue to thrive within the climate zones where they now grow. Another goal is to adjust or take advantage of zones in which they cannot grow but soon might. Collectively, these challenges constitute a huge and perhaps overwhelming task for plant breeders. Plants routinely establish relationships with microorganisms, some of which may be familiar while others are little recognized or appreciated. Nodule For instance, nitrogen-fixing bacteria typically live in nodules along the roots of leguminous plants, forming a mutually beneficial relationship. Bacteria are not the only members of the microbial world to form close partnerships with plants. Many fungi and viruses also form such partnerships. Some of these relationships between plants and microbes, which developed over millions of years, are close to being harnessed on a commercial scale to support crop growth, according to several experts who spoke at the ASM General Meeting session. 113 For instance, arbuscular myccorhizhal fungi (AMF) live within plant roots, from which they send out filaments that collect phosphate, a critical nutrient for their host plants, according to Ian Sanders from the University of Lausanne in Switzerland, who spoke during that session. These fungi, which microbiologists first recognized about 40 years ago, are "found in all soils," where they form "symbioses with plant roots," he says. When scientists applied AMF to crops years ago, they conducted those field trials in North America and Europe where plants grow well with conventional phosphate fertilizers, he says. Thus, adding the fungi had little effect, "and hardly anyone uses them." Farming in the tropics is another story, Sanders continues. "In the tropics, farmers need to add huge amounts of phosphates," he says. "All farmers add phosphate, but we're aiming to increase yields and reduce the amounts of phosphate being added." His research involves selecting and adapting isolates of AMF that will thrive in tropical soils, improve uptake of phosphate along the roots of plants being grown commercially in those regions, and testing whether adding such fungi will improve yields of crops such as cassava, rice and potatoes. "Those crops form natural [symbioses] with myccorhizhal fungi, and we're adding more and modifying the genetics hopefully to get better yields," he says. There are some promising results, particularly with potatoes being grown in test plots by his collaborators at the National University of Colombia, according to Sanders. Yields of potatoes grown with AMF remain steady, but those plants require only 38% of the phosphate fertilizer that is usually added to this crop plant, he says. This savings in use of phosphate fertilizer is additionally important because intensive use of phosphate comes with an "environmental cost" and "phosphate reserves are being depleted," he points out. Meanwhile, in other experiments in Colombia in which such fungi are added to rice, there is a 20% increase in yield, according to Sanders. "We never expected this increase in yield, but it's very encouraging," he says. The prospects are bright for using AMF in many settings involving crop plants, in part because of the inherent genetic diversity of such fungi, Sanders adds. Each fungal cell contains thousands of nuclei with a range of genetic variation distributed among those nuclei. By crossing fungi and allowing nuclei to segregate into separate spores, it is possible to develop novel fungal lines to evaluate for their growth effects on host plants. Improved lines are sent to a biotechnology company that has methods for expanding fungal lines and for packaging them in a proprietary gel that makes it easier to ship and apply the material to plants in fields. 114 Some Microbial Products Reduce Stress Pathways in Host Plants Bacteria with the gene encoding the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase can protect host plants against a variety of stresses, including drought and flooding, heavy metals, high salinity, and pathogens, according to Bernard Glick from the University of Waterloo in Ontario, Canada, another speaker during the plenary session. ACC deaminase acts by damping a major stress response pathway in plants. When plants are stressed, they produce ethylene gas, which plays many roles in plant growth and development, including stimulating root lengthening and fruit ripening. However, when plants produce ethylene in response to stress, root growth stops, leaves fall off, and fruit production slows. While these effects may be protective in the wild, all of them reduce productivity in agricultural settings. Because ACC deaminase inactivates an ethylene precursor, plants no longer can produce the gas, even when stressed, and productivity remains high. Another approach easing drought stress on plants involves bacteria that make the sugar trehalose, according to another plenary session speaker, Gabriel Iturriaga from the Universidad Autonoma del Estado de Morelos in Mexico. Helping crop plants withstand drought is critically important because high temperatures and salinity affect more than 30% of arable land, reducing crop yields by up to 50%. Some naturally drought-tolerant plants produce trehalose, which stabilizes membranes and enzymes, protecting them against damage when cells are subjected to repeated cycles of drying and rehydration. Although the capacity to synthesize trehalose is rather scarce among plants, many microorganisms, including bacteria and fungi, can synthesize this simple disaccharide. "We were surprised to find several genes for the biosynthesis of trehalose in many plants that do not come from arid environments, where those genes are tuned down or silent," Iturriaga says. Inducing such plants to produce extra trehalose makes them resistant to drought. However, it might prove more effective to use plant-associated bacteria to provide the hosts with trehalose, instead of engineering plants to make more of the disaccharide, according to Iturriaga. In pursuit of that strategy, he and his collaborators induced Rhizobium etli, a bacterium that grows within the roots of bean plants, to overexpress trehalose. Plants carrying the trehalose-producing bacteria prove more resistant to drought and produce more foliage and deeper roots, he says. The productivity of such plants increases by more than 50% under normal conditions, and continues to produce at least 50% of normal yields under conditions of drought. Meanwhile, the productivity of plants without the trehalose-producing bacteria drops to nearly zero. Inoculating corn with trehalose-producing Azospirillum brasilense also improves drought tolerance and productivity, Iturriaga continues. Curiously, the drought resistance in the corn and bean plants does not correlate with increased trehalose production. Instead, the trehalose apparently signals several stressresistance pathways in the plants, he says. 115 Unexpectedly, Some Viruses Improve Plant Productivity If the notion that bacteria or fungi can improve plant productivity takes some getting used to, what about the idea that viruses also improve plant productivity and provide help toward reducing stress? However, symbiotic viruses help to explain how some plants manage to grow in soils next to hot springs in Yellowstone National Park, according to Marilyn Roossinck from the Noble Foundation in Ardmore, Okla. Soils surrounding such hot springs sometimes reach temperatures greater than 50oC (122oF), well above the usual limits for vascular plants. Nonetheless, panic grass thrives, growing in clumps surrounding hot springs. Panic grass survives by growing in symbiosis with an endophytic fungus, Curvularia protuberata. Plants with C. protuberata in their roots can survive soil held at 65oC for 10 days, Roosinck says. However, the fungus itself is not equipped to protect the plant, she finds. In the wild, that fungus is infected with a doublestranded RNA virus, and if the fungus is "cured" of the virus, its host plant can no longer withstand hot soils. Somehow this virus, whose genome encodes only five proteins, enables the fungus and the plant to survive high temperatures. Other kinds of plants living in hot volcanic soils in Central America carry fungus-virus pairs similar to those found in the Yellowstone panic grass, she adds. Roossinck is studying the molecular basis of these partnerships with the goals of better understanding how fungi-viral pairs can enable host plants to withstand hot soils and then using this information to apply to crop plants. In more general terms, endophytes from native plants growing in harsh environments may prove useful if they can be matched with, adapted to, and proved protective for commercially important plant crops, she says. 116 Some Fungi Protect Plants against Damaging Insects and Pathogens Fungi may help increase crop productivity and, thus, the food supply in another way--by reducing waste, according to another session participant, Gary Strobel of Montana State University. "For fun and work, I go to jungles in the equatorial regions of the world to find new microorganisms," he says. "I've been to rain forests, the tropics, and temperate zones looking for low-hanging ‘fruit' to bring back to the lab." Like Roossnick, he has a particular interest in endophytes, particularly filamentous fungal species that live on and within plant tissues, forming many different kinds of relationships with their host plants. About 12 years ago, Strobel returned from South America with plant samples that were infested with mites. After placing cuttings on agar to encourage endophyte growth, he put the materials in an airtight box for 12 days that, once opened, gave off a strange odor and contained a white fungus, which he later named Muscodor albus-"stinky white fungus." Indeed, this fungus produces a mix of about 30 volatile compounds, most of which are harmless in themselves, but collectively prove profoundly antimicrobial. Calling it as effective as bleach but safe enough to drink, Strobel says the mixture "is ready to go for decontaminating fruits and vegetables" and also "can be used to take care of biofilms." The fact that activity resides in a mix of chemicals is "important," he says. Many researchers "follow a mindset" of looking for single molecules with antimicrobial activity, "but nature doesn't work that way; it's a mixture." Other endophytic fungi that Strobel collected during his travels produce a diverse array of compounds with an equally diverse array of activities. "This field [of research] is enormous, and we just don't know how these microbes interact with plants," he says. "All kinds of barriers say this research is impossible." However, he urges others to take up many of its challenges, which include traveling to exotic locales to collect specimens, braving diseases and discomfort, conducting the chemical assays and the microbiological workups, and writing the patent applications to protect intellectual property needed to commercialize it. For the 1 billion people who face starvation, this emerging field of microbiological research "can really contribute" toward helping to "provide more food," adds Sanders from the University of Lausanne. "For those of us in microbiology, we need to use what we have and also find new things." 117 BACKGROUND OF THE COMPANY 118 Billion Microbes International Sdn Bhd (BMISB) (Company No:1078763) is a Private Limited Company that owns the marketing rights of the Microbe product known as “BillionMicrobes” (BM) (which was previously also known as Baja AGX (AGX Fertilizer) which is a Biological treatment product in liquid form comprises of a mixture of 30 different types of Micro Organisms with Nutrients and Stimulants which function as a booster to crops and which increases the crops immunity towards diseases, both for the local market as well as the international market. BMISB is the Sole Distributor for all the products under the brand name Billion Microbes which is being trademarked. Dr. Frank Ow Yang Abdullah (Dr. Frank) who is a Surgeon with the following qualification MBBCh LRCP & SI BAO (NUI) from the Royal College of Surgeon in Ireland who is also the inventor of the BM products together with another Scientist and who is a specialist in Agronomy and an expert in Microbial Agriculture Technology & Organic Recycling. The ownership of the BM is under Pondscapes Sdn Bhd (PSSB) (Company No: 504227-W). PSSB was incorporated since March 2011 in Petaling Jaya, Selangor by Dr. Frank and Puan Liana Ow Yang Abdullah and the said Company’s main activity is focused on Landscape Designs, Projects and Maintenance.The Company has an outstanding record of performance and has received various awards. As a result of Dr. Frank’s relentless hard work and effort, they succeeded in inventing and developing a Microbe based product which is a very effective crop booster which increases the crops endurance in resisting plant related diseases thus resulting in increased yield. Dr. Frank is also qualified as a Medical Physician/Specialist and a General Surgeon who graduated from the Royal College of Surgeons, Ireland (UK). After graduating Dr. Frank had served in the medical fraternity as a Specialized General Surgeon in Beaumont Hospital, Dublin, Ireland (at that time one of the most advanced hospitals in Europe) for several years before returning to Malaysia. Upon returning to his beloved homeland Malaysia in 1995 and doing landscaping for several years, Dr. Frank commenced his research and analysis in Microbial Agriculture Technology for more than twelve (12) years, before discovering a magical formula which is very effective and vigorous namely Billion Microbes. Finally, this product was successfully manufactured sometime in 2009 and marketed since 2010 onwards. OUR MISSION AND VISION Our Mission is to increase the production of yield, strengthen the crops resistance and to overcome the numerous problems in the Nation’s Agricultural Sector especially against the variety of diseases involving crops generally. With our successful invention of this formula in Microbial Agriculture Technology it is effective in that it has opened our minds and brought about awareness to all consumers and farmers regarding the importance of this technology especially today in the 21st Century, wherein pollution and the use of Chemicals on land and soil in agriculture has now reached very dangerous levels and thus alarming. In the meantime, should Agricultural yield be increased by the use of this Microbes based product it will also increase the income of the Country and the World. Notwithstanding the same, it will also enhance social security in the country and in the world, due to the increased yield of the farmers by leaps and bounds (which has been achieved and obtained repeatedly in our exhaustive field tests) and sustain future generations of farmers and planters in their fields. 119 OUR INITIAL ACHIEVEMENT (PONDSCAPES SDN BHD) MULTIPLE AWARD WINNER, by DR. FRANK 1. BEST LANDSCAPING AWARD FOR BUNGLOW CATEGORY IN TROPICANA 2000/2001” WINNER FOR 1st & 2nd PRIZE 2. SECOND BEST IN NATIONAL LANDSCAPE COMPETITION 2003, FOR CONTEMPORARY JAPANESE GARDEN & LANDSCAPE CARPARK IN WEST PORT MALAYSIA, PULAU INDAH, KLANG, SELANGOR. 3. ANUGERAH PRESTIJ IDEA DEKORASI IMPIANA 2010 ANUGERAH KEDIAMAN PEMBACA MAJALAH LAMAN ANUGERAH PRESTIJ KATEGORI BANGLO Anugerah disampaikan oleh: Y.B Dato’ Sri Ismail Sabri bin Yaakob Menteri Perdagangan Dalam Negeri, Koperasi dan Kepengunaan 2010. 120