Brochure "Naticol"

Skin Care-Anti-ageing
Healthy Joints
Energy-Sport nutrition
Low carb meals &snacks
®
Hair care products
1000
®
4000
Part I. What is NatiCol ?
®
The Weishardt company introduces,
NatiCol® 1000 and NatiCol® 4000,
a complete range of marine hydrolyzed
collagen
NatiCol® is selectively derived from
fish skin & scales which have been
consumed by mankind for a long time.
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Part I. NatiCol Flow chart
®
Enzymatic
hydrolysis
Purification
Extraction
Weishardt
Internationa
l is certified
Acid treatment
Degreasing
ISO
9001/2000
100% Fish &
Scales skin
Filtration
Concentration
Sterilisation
Spray-drying
®
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Part I. NatiCol Traceability
®
Weishardt controls
each step and can
guarantee total
traceability from the
farm to the delivered
batch.
Integrating the fish activity from beginning to end, Weishardt
controls each step. All raw material is veterinary controlled.
Page 4
Part I. NatiCol safety
®
Applying gelatine process affords the best security.
2 sterilisations assure the best bacteriology.
Ionic demineralisation and filtrations the maximum purity and
highest collagen ratio.
Weishardt, while purchasing various species worldwide guarantees
the best long term security, for you and your customers.
®
Choosing NatiCol means also avoiding some risks and questions
regarding BSE for bovine collagen, avian flu for chicken collagen or
any epizootic disease which could appear localy.
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Part I. NatiCol composition?
®
NatiCol contains:
®
・　93-95% protein mainly
NatiCol is:
®
collagen peptides type I.
・ Less than 6% water
・ Less than 2% mineral salts 
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Carbohydrate FREE
Fat FREE
PURINE FREE
CHOLESTEROL FREE
OGM FREE
PRESERVATIVE FREE
Part I. NatiCol molecular weight
®
Weishardt has developed two molecular weights covering the main
market needs and adaped to various applications.
NatiCol® 4000 is close to usual Marine collagen found on the market
(Av<4000Da). Its very high organoleptic properties make Naticol
4000 one of the most appreciated and make the difference!
NatiCol® 1000 is obtained through a special process in order to obtain
low molecular weight short chains of peptides (Av<2000Da)
It is particularly adapted to topic applications.
Due to its faster assimilation by organism, it can be also adapted to
some oral applications.
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Part I. NatiCol 1000 specificity
®
Molecular weight Distribution
(MWD)


0,3-1 Kda minimum 40%
1-2 Kda
30%
®
Brand X
1000
NatiCol® has an
extraordinary level of
low molecular weight
which makes it so
unique compared to
conventional collagen
®
4000
3-5 Kda
0,3-1 Kda
Page 7
MWD
(Kda)
Part I. NatiCol
®
Tyrosine
Amino-acid (AA) composition
85.5%
Hydroxylisine
NatiCol® contains:
Histidine
AA (%)
Serine
・　27.6% of Glycine
・　23% of Proline & Hydroxyproline
Arginine
Alanine
Ac.aspart ique
Ac.glutamique
which are the main components of skin
Hydroxyproline
Proline
Glycine
0
5
10
15
20
25
30
Essential AA(%)
Valine
14.5%
Tryptophane
NatiCol® contains:
Tréonine

Phenylalanine
Methionine

Lysine
Leucine
Isoleucine
0
0,5
1
1,5
2
2,5
3
3,5
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8 essentials amino- acids
which represents
14,5% of the total AA
Part I. NatiCol
®
Amino-acid (AA) composition
Arginine
Histidine
Hydroxyl isine
Lysine
Scales
Tyrosine
Phényl al anine
Naticol
Leucine
AA composition varies slightly
according to age, food and origin.
I soleucine
Méthionine
Cystéine
Val ine
Al anine
Gl ycine
Hydroxyprol ine
Prol ine
Gl utamic acid
Sérine
Thréonine
Aspartic acid
0,00
5,00
10,00
15,00
20,00
25,00
30,00
CHEMICAL INDEX ( way to look for the ideal protein)

Naticol index is 146 bearing in mind that above 100 means complete
protein. Only the absence of Tryptophane is a limiting factor as for all
collagens.

The index of amino-acids (Pro, Hypro, Gly) necessary for skin is double
that of soy or milk protein.
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I. NatiCol solubility
®
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Excellent solubility in cold water (5%)
Highly dispersible (up to 30% of MG)
Very good clarity
®
®
Brand X
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100% Natural Fish Collagen
2 types: Powder (Pd) & Micro-granulated (MG)
White powder, Odourless
Part I. NatiCol Key benefits
®
■
■
■
■
■
■
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100% Natural Marine product
2 types: Powder & Micro-granulated
93 to 95% of pure Collagen
High level of oligo-peptides
High level of essential amino-acids
Neutral taste and odour
Excellent solubility in cold water
Halaal certified
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Part II. NatiCol Skin benefits
®
NatiCol is a natural source of collagen type I, the
Epidermis
major protein found in connective tissues and
skin (more than 75%) which plays an essential
role in maintaining skin tone, suppleness &
Dermis
elasticity. Unfortunately after the age of 25
collagen synthesis reduces at a rate of 1,5% a
year. By the age of 45 collagen levels can have
fallen by as much as 30% 1,2,3.
Hypodermis
The external effects of this process are the
appearance of wrinkles, fine lines & dry skin
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Part II. NatiCol Skin benefits
®
• NatiCol provides a pure & concentrated level
of marine collagen (95%). Used in rejenuvating
formulaes it help to replenish the body’s own
collagen and provides essential nutrients to rebuild collagen cells due to its small molecule
(tripeptides) and its high bioavailability.
• Regular use of products integrating NatiCol
marine collagen may help slow the signs of
ageing by increasing Collagen synthesis, skin
elasticity & firmness and reduce fine lines and
wrinkles .
Page 13
Part II. NatiCol
Skin care markets &
applications
®
Applications
Skin care markets
Skin Anti-ageing
Anti-wrinkle effect
Reduce fine lines
Skin firmness
Skin elasticity
Increase collagen synthesis
1. Topical cosmetic
products
2. Oral cosmetic
products
Page 14
Part II. NatiCol Cosmetic use
®
COSMETIC USE
•
•
NatiCol® can be used in topical cosmetic anti-age and skin care
preparations (cream, lotions, emulsions).
NatiCol® can be used in oral cosmetic «Cosmetofood» anti-age and
skin care preparations (tablets, soft gels, beauty drink..)
SAFETY/TOLERANCE
NatiCol® is 100% safe and has a good tolerance.
DOSAGE/FREQUENCY
1.
2.
4.
3.
Topical Dosage: 0,2 to 2%
Oral Dosage: 5g per day
Frequency of use: Daily or twice daily for 2-4 months
Solubility : NatiCol is soluble in water, by stirring at room temperature.
Page 15
Part II. NatiCol
Cosmetofood suggested formulations
®
Example 1: Cosmetic supplements (tablets,
for anti-wrinkle and anti-ageing effect
•NatiCol® (marine Collagen)
•Vitamin C
•Vitamin A (retinol)
• OPC (grape seed extract)
Example 2: Cosmetic supplements
(sticks)
for instant drinks
•NatiCol® (marine Collagen) : 800mg
• Acerola extract (vit C)
: 100mg
• CoQ10
: 10 mg
• Marine Chondroitin sulfate
: 100 mg
• Flavour
Page 16
:1000mg
: 100mg
: 50 mg
: 50 mg
capsules)
Part II. NatiCol
Cosmetofood suggested formulations
®
Example 3: Cosmetic Supplements for Skin Whitening &
Anti-ageing effect
•NatiCol® (marine Collagen)
•Fytoperfect (skin whitening complex)
•Vitamin C
•Vitamin E
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:800mg
: 800mg
: 100 mg
: 100 mg
Part II. NatiCol
Cosmetic suggested
formulations
®
Example 4: Cosmetic gel for anti-wrinkle effect
• NatiCol® (95% pure marine Collagen)
: 2%
• Other ingredients: Carpobol , Glycerin, Perfume, sodium sulfate,
ethanolamin, preservatives
Example 5: Cosmetic cream for anti-wrinkle & moisturizing
effect
• NatiCol® (95% pure marine Collagen)
: 2%
• Other ingredients: Vitamin E, Soy& Advocate oil, Glycerin,
perfume, sodium sulfate, preservatives….
Page 18
Part II. Bibliography
•
Babraj JA et al 2005: Collagen synthesis in human musculoskeletal tisues and
skin. Am J. Ph. Endocrinol Metab. 289 (5) : 864-9.
•
Fligiel SE et al (2003): Collagen degradation in aged/phodamaged skin in vivo and
after exposure to matrix metalloproteinase-1 in vitro. J. Invest. Dermatol. 120, (5)
842-8.
•
Chung JH (2001): Modulation of skin collagen metabolism in aged and photoaged
human skin in vivo. J. Invest. Dermatol. 117, (5): 1218-24.
•
Reuther T et al (2004): Active ingredients in dermatocosmetics. Hautartz.. 55 (7)
:630-6.
Page 19
Part III. NatiCol : Function in

healthy joints
p
i
Ep
is
s
hy
Synovial
membrane
Articular
cartilage
surface
Joint
capsule
Epip
hys
is
Both ends of the bone
(epiphysis) -covered by the
articular cartilage- are kept in
position by the joint capsule.
The synovial membrane binds
the 2 articular surfaces and
secretes a synovial fluid which
ensures cartilage lubrification.
Part III. NatiCol- Normal Articular
Cartilage
Chondrocytes(Joint cells)
•Articular cartilage is a connective tissue
made of cellular components (Chondrocytes)
and an extracellular matrix , formed by
collagen fibres and a gel called fundamental
substance.
•This « fundamental substance » contains
free proteoglycanes and free
glycoproteines..
•Proteoglucanes are large molecules made
up of a long chain of hyaluronic acid bound
with protein and GlycoAminoGlycans (mainly
Chonroitin sulfate).
Extracellular matrix
Part III. What are Chondrocytes?
Chondrocytes are cells that help cartilage to
grow and stay healthy.
They synthesize the collagen and proteoglucanes
that form the extracellular matrix, the basis of
the cartilage tissue
Part III. What are GAGs?
GlycosAminoGlycanes(GAGs)-previously called
mucopolysaccharides-are large molecules formed by the
repetition of more than 100 polyssacharide units.
Chondroitin sulfate(CS) is the main GAG found in the
articular cartilage.
CS is a glucoside sulfate polymer (50-100 Kda) formed by the
repetition of dissacharide units of galactosamine and
glycuronic acid.
CS has a strong electronegative structure which confers a
high hydrophobic capacity.
Part III. What is Collagen?
•Collagen is an essential and major component of connective
tissues (joints, tendons, cartilage, ligaments, blood vessels), and
makes up 25% of the total protein mass.
•It is a complex trimer structural protein with a high level of
glycine and proline and morever contains Hydroxyproline and
Hydroxylysine found in very few other proteins
• There are 3 main types of collagen:
•Type I and III (found mainly in skin, tendon and bone)
•Type II (found exclusively in cartilage)

Part III. NatiCol Healthy Joints markets &
applications
Joints markets
1. Senior Joint Health
2. Sportsmen Joint health
3. Menopausal women
Applications
1. Osteoarthritis
2. Healthy Joint functions
3. Osteoporosis
Part III. What is Osteoarthritis?
Osteoarthritis (OA) is the most common form of age-related
disease in people aged 65 and older.
It is a degenerative joint disease characterised by the
breakdown of joint articular cartilage, triggering pain, stiffness
and swelling.
Rheumatoid arthritis (RA) is an autoimmune disease different
from OA. In fact, the immune system attacks the body’s own
tissue as though it were a foreign invader.
People with RA produce rheumatoid factor, an immunity-related
substance that targets the synovial membrane (protective layer that
encases joints). The consequences are severe pain and inflammation,
joint pain and loss of joint movement & functions.
Part III. How does NatiCol work?
Clinical studies have shown that hydrolysed collagen targets
and has a positive effect on joint tissue 1, 2, 3,4, 7.
Daily supplements of 6-10 g of hydrolysed collagen are
generally admitted to reduce pain and improve mobility
when use with complementary ingredients.
It has been suggested that hydrolysed collagen stimulates
chondrocyte production, which is involved in the
biosynthesis of type II collagen, essential to new cartilage
formation. 1, 2, 4, 6,
Part III. Bibliography
•
Oesser S Seifert J. (2003): Stimulation of type II collagen biosynthesis and
secretion in bovine chondrocytes cutured with degraded collagen. Cell Tissue Res.
311 (3) : 393-9.
•
Oesser S et al (1999) : Oral administration of 14C labeled gelatin hydrolysate
leads to an accumulation of radioactivity in cartilage of mice (C57/BL). Nutrient
matabolism 129, 1891-1895.
•
Deal Cl, Moskowitz RW (1999): Nutraceuticals as therapetic agents in
osteoarthritis. The role of glycosamine, chondroitin sulfate, and collagen
hydrolysate. Rheum Dis Clin North Am. 25 (2) : 379-95
•
Moskowitz RW (2000): Role of Collagen hydrolysate in bone and joint disease.
Semin Arthritis Rheum.. 30 (2) : 87-99.
•
Takeda et al 1982 (1982) : ACute and subacute toxicity studies on collageb
wound dressing (CAS) in mice and rats. J Toxicol Sci. 7 (2): 63-91.
•
Pesakova V, Stol M, Adam M. (1990) : Comparison of the influence of gelatine
and collagen substrates on growth of chondrocytes. Folia Biol. 36 (5): 264-70.
•
Novotna et al. (1991): The role of cartilage minor collagens in inducing arthritis.
Z. Rheumato. 50 (2): 93-8.