Agela Catalog 2009-2010

Transcription

Agela technologies
Chromatography
Catalog
2009-2010
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Agela Technologies — A Global Supplier for Chromatography Solutions
2008 Message From Agela Technologies
As Agela technologies is poised to enter its fifth year with confidence and pride in its innovative
separation, purification, and sample preparation products, we would like to thank our many loyal
customers for your continuous support and trust. With your support and our effort in delivering the highest
quality products to you, our company has grown remarkably. This has allowed us to expand our research
and development effort, and thus introduce more innovative products to better service your application
needs.
In 2007, we had tremendous accomplishments: We cataloged over one thousand different products. Our
manufacturing and R&D operation was certified in compliance with ISO 9001 and passed many quality
audits by customers and distributors, including VWR. As a global wide company, we not only have our
own international sales force but have also formed a marketing alliance with VWR international. This will
allow us to reach higher goals for 2008 and to provide our customers with even better quality products
and faster service.
Our 2008 mission statement and commitment:
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Provide products with our INNOVATIVE technologies at the best PERFOR MANCE to COST ratio.
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Deliver products with guaranteed quality.
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Provide global support with quick responses
How to Place Orders
Our office is open from 9:00 am to 6:00 pm Eastern Standard Time, Monday through Friday.
To place an order or receive a quote, you may choose from the following contacts:
Mail Address: Agela Technologies Inc.
625 DAWSON DRIVE, Newark, DE19713
Phone: (302) 438-8798
Fax: (302) 235-1833
Email: [email protected]
On-line: www.agela.com
Please include the following information with your order or request: Account number (if you have one),
purchase order number, contact name, organization name, shipping and billing address, telephone
number, fax number or email address, product number, brief description and quantity, method of
payment, preferred method of delivery. A written confirmation will be sent to you by email or fax. We
accept business checks, wire transfers, and major credit cards as methods of payment.
Checks:
Please make checks payable to:
Agela Technologies Inc. and send to:
Agela Technologies Inc.
625 DAWSON DRIVE, Newark, DE19713
Wire Transfer:
Please contact us by phone, fax or email for account information.
Credit Cards:
Please include the card type and number, expiration date,
and card holder name. Due to security concerns, please
do not email the information. Please call or send a fax to
provide your credit card information.
Terms and Conditions
PLEASE READ THESE TERMS BEFORE ORDERING. IF YOU HAVE ANY QUESTIONS, PLEASE DO
NOT HESITATE TO CONTACT US AND OUR STAFF WILL BE GLAD TO ASSIST YOU.
Acceptance and Availability
All orders placed are subject to the agreement of Agela Technologies Inc. The catalogue does not
constitute an engagement of the company to sell all listed products. You are guaranteed to be notified at
the time of ordering if the ordered items are in back-order or discontinued.
Price and Payment
The prices are in effect at the time of printing. Agela Technologies reserves the right to change the prices
without notice, though we do our best to provide our customers with advance notice. The prices quoted at
the time of ordering will be guaranteed. The general payment term is net 30 days, F.O.B., Newark,
Delaware, USA. However we reserve the right to ask for prepayment if customers’ account information is
not satisfactory. A 1.5% per month service charge will be added to delinquent accounts. If a purchase
order is less than $1000.00, a $50.00 extra charge will be added to the invoice.
Changes
Agela Technologies reserves the right to change product specifications, quantities, designs or prices
without prior notice and without liability for such changes.
Shipping Policy
The standard shipping method is 2 day FedEx within the United States and Canada. We will try to
accommodate requests for other shipping methods if they are available. All shipping and handling
charges will be billed separately. Should you receive damaged goods, it is imperative that you notify us
immediately and save all packing materials for inspection by the carrier.
Application
All products in this catalog should be used for laboratory or manufacturing use only. They are not
intended for direct medicinal or food use. Agela Technologies assumes no liability for any misuse of the
products.
Returns
Agela Technologies tries to accommodate all requests for returns of unused goods. However, return of
some items may be restricted by the original manufacturers. Please contact us for return authorization
before returning any items. A restocking charge may be applied to certain products.
Warranty
All Agela Technologies products are warranted to be free of defects in materials and workmanship. They
are not warranted for any other particular purpose. Agela Technologies shall not under any circumstance
be liable for any incidental, consequential or compensatory damage in conjunction with its products. The
maximum liability shall not exceed the invoice price of the product.
CONTENTS
HPLC Columns and Media
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Introduction
-------------------------------------------------------------------HPLC Columns by Family
----------------------------------------------------Selected Solutions for Popular Applications
------------------------------Ordering Information by the Type of Stationary Phases
---------------HPLC Column Selection Guide
----------------------------------------------Applications
-------------------------------------------------------------------Appendix
-----------------------------------------------------------------------
002
002
033
044
054
058
075
SPE Columns and Wellplates

Cleanert SPE Columns,Wellplates and Media
----------------------------The Development of SPE Method
-----------------------------------------Applications
-------------------------------------------------------------------Cross-reference Table
--------------------------------------------------------
078
103
106
114
Purification Products

Preparative HPLC Columns
-------------------------------------------------- 116
Flash Chromatography Media and Columns
------------------------------ 120
Flash Chromatography System
--------------------------------------------- 125
GC Columns
126
Accessories and Supplies
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HPLC Accessories and Supplies
---------------------------------------------SPE Accessories and Supplies
----------------------------------------------GC Accessories and Supplies
-----------------------------------------------Filtration Products
------------------------------------------------------------Vials and Caps
-----------------------------------------------------------------
146
153
157
162
166
HPLC Columns and Media
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Introduction
-------------------------------------------------------------------- 002
HPLC Columns by Family ---------------------------------------------------- 002
Unisol Technology and Column Products–NEW
---------------------------------Optimix Technology Column Products–NEW
------------------------------------Durashell Technology and Column Products –NEW
-----------------------------Venusil Family of HPLC Columns
------------------------------------------------Promosil HPLC Columns
---------------------------------------------------------Other Manufacturers’ Brands
-----------------------------------------------------
002
011
015
017
031
032
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Selected Solutions for Popular Applications
033
033
039
040
042
043
043
043
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Ordering Information by the Type of Stationary Phases
044
044
047
049
050
051
051
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HPLC Column Selection Guide
---------------------------------------------- 054
Applications
-------------------------------------------------------------------- 058
Appendix
------------------------------------------------------------------------ 075
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------------------------------Solutions for Highly Water Soluble Compounds
---------------------------------Solutions for Low pH and High pH Applications
---------------------------------Solutions for LC-MS
-------------------------------------------------------------Solutions for Fast Analysis
------------------------------------------------------Solutions for Bio-molecules
-----------------------------------------------------Solutions for Preparative HPLC
-------------------------------------------------Solutions for SFC
----------------------------------------------------------------------------Reverse Phase Columns
--------------------------------------------------------Normal Phase Columns
---------------------------------------------------------Ion-exchange Columns
---------------------------------------------------------Mixed Phase Columns
----------------------------------------------------------Size Exclusion Columns (Silica Based)
------------------------------------------Polymer Phase Columns
---------------------------------------------------------
Introduction
As one of the emerging leaders in chromatography technology, Agela Technologies
proudly presents five new HPLC columns developed using our latest cutting-edge
technologies. These products exhibit superior performance and are designed to meet the
ever-increasing demands of our customers.
1.Unisol C18 - A type of column that is a true universal reverse phase column for the separation of acidic,
basic, and neutral molecules. It has superior performance to any conventional C18 phases in almost
every aspect.
2.Unisol Amide - A unique phase that is very different from other stationary phases in its separation
mechanism. It can be used as a RP or a NP column. It has superior performance than any of the other
HILLIC columns on the market. It has superior retention for polar compounds when operated as a HILIC
column.
3.Optimix columns – A class of columns that provide optimized selectivity and performance for HPLC
separations. It has balanced hydrophilicity and hydrophobicity: extended retention for polar compounds;
non-excessive retention for strong hydrophobic compounds.
4.Durashell columns - A class of column that can be used in a wide pH range of 1-12.
5.Venusil ASB columns – A class of columns that are excellent for low pH and LC-MS applications.
HPLC Columns by Family
Unisol Technology and Column Products
NEW in HPLC !
Agela’ s unique sol-gel process technology generates a uniform surface that produces a universal
column applicable to many HPLC applications. Peak tailing adversely affects chromatographic efficiency
and reduces the resulting accuracy and precision of the data. Peak tailing and insufficient retention of
polar compounds are among the toughest problems for silica based reverse phase columns. High acidity
of the surface silanols, non-uniformity of the surface, and non-uniformity of the bonding process are the
three major causes of peak tailing.
High purity synthetic silica is typically obtained by hydrolyzing silicic acid esters followed by high
temperature sinteration. The sintered silica is further leached with dilute acid to enrich silanol on the
surface. The process does not generate a uniform, silanol covered surface. It will create some isolated
silanol groups with very strong acidity or some “un-leached islands” during the leaching process.
Furthermore, the high temperature procedure may cause formation of micro-crystallization domains on
the amorphous silica, further contributing to the nonuniformity of the surface.
Unisol process technology is specifically designed to solve these problems. In our patented Unisol
process, gas phase tetramethoxy silane is adsorbed evenly on the silica surface. It is subsequently
hydrolyzed by a controlled amount of moisture. A fresh layer of silanols is then formed by a mild heating
process. This process produces a surface with lower acidity and high uniformity, which is further bonded
with a high purity C18 silane, and then di-functional end-capped. The overall process yields an excellent
HPLC stationary phase with a very smooth surface that is friendly to basic compounds (minimal ionic
interactions) and polar compounds (provides significantly increased retention).
Major benefits of the Unisol Technology for reverse phase and normal phase chromatography:
●Greatly improved peak shape for basic compounds
●100% water compatibility
●Significantly increased retention for highly water soluble compounds and balanced hydrophobicity in the
reverse phase mode
●Elimination of excessive retention for polar compounds in the normal phase mode (“hot spots”)
Balanced Retention for Hydrophilic and Hydrophobic Compounds
Mobile Phase: 35% 20 mM KH2PO4 pH=7.0，65% Methanol
Temperature:
23℃
Flow:
1mL/min
Detection:
UV 254 nm
Sample:
Uracil
Propranolol hydrochloride
Butyl Phthalate
Dimethyl Phthalate,
Naphthalene
Acenaphthene
Amitriptyline
Extremely Low Metal Effects
Mobile Phase: A: Water, B: Methanol; A:B = 65:35
Flow Rate: 1 mL/min
Temperature: 25℃
Detection: UV 230 nm
Sample: 2,3-Dihydroxynaphthalene+2,7-Dihydroxynaphthalene
Unisol C18 5μm, 4.6mm×150mm
Brand W, AQ Column 5μm, 4.6mm×150mm
VWD1 A, Wavelength=230 nm (LPF\07091702.D)
Brand H, C18 Column 5μm, 4.6mm×150mm
Brand Z,C18 Column 5μm, 4.6mm×150mm
Unisol C18
A Slightly Polar, 100% Water Compatible and Universal Reverse Phase
A unique and universal C18 HPLC phase made by our patented Unisol Technology. Unprecedented
separation performance for compounds with a wide range of properties from hydrophilic to hydrophobic:
polar, semi-polar and non-polar compounds.
Characteristics: Metal Impurity < 10 ppm; Pore Size 100 Å; Specific Surface Area 410 m2/g; Carbon
Loading 17%; Single end-cap; Available Particle Size: 2.5 μm, 3μm, 5μm, 10μm
Consider this column as the first option for your HPLC method development
●One of the most universal reverse phase columns
●Great inertness and efficiency for basic compounds
●Enhanced retention of hydrophilic compounds
●100% aqueous compatibility
●Robust and reproducible performance
●Wide pH range (1.5-9.0)
●Low bleed and high sensitivity for LC-MS
Great Inertness and Efficiency for
Basic Compounds
Good Retention for Polar Compounds
Mobile Phase： A: Water (Contain 0.01mM
Na2PO4 and 0.01mM Citric Acid, pH=4.5 )
B: ACN , A:B =95:5
Sample:
Cytosine, Uracil, Guanine
Temperature： 35℃
Sample: uracil, toluene, doxepin, amitriptyline
Column: 4.6 x 150mm, 5 μm
Mobile phase: 10 mM sodium phosphate (pH =7)
in 60% ACN.
Temperature: 30℃; Flow rate: 1 mL /min.
The plate numbers in regular font are for toluene,
the plate numbers in Italic are for amitriptyline
Detection：
254 nm
Good Tolerance of Directly Injected Biological Matrix
Sample:
uridine in calf blood plasma (diluted 20
times with 0.1% formic acid)
Injection:
1μl (not be cleaned up with SPE)
Column:
Unisol C18 2.1×50mm,5μm
Temperature: 30℃
Flow Rate:
0.3mL/min
Mobile phase: 0.1% formic acid solution (100% aqueous
phase)
Run time:
8 min
After 500 injection, wash with methanol /0.1% formic
acid solution = 50/50 for 30mins，column temperature 40
℃; rinse with 95%MeOH for 30mins, column
Enable Methods to be Compatible with LC-MS
Analysis of Quaternary Ammonium Alkaloids from
Copitidis with a Non-Volatile Ion Pair Reagent using
Brand Z C18 Column.
Mobile Phase: ACN: 0.05 mol/L KH2PO4 Aq.
(40∶60, Contain 0.015 mol/L SDS)
Analysis of Quaternary Ammonium Alkaloids from
Copitidis without a Non-Volatile Ion Pair Reagent
Using Unisol C18 Column .
Mobile Phase : Water + 0.2％ TFA :acetonitrile= 75:25
Compatibility with 100% Aqueous Mobile Phase
Sample:
Column:
Uridine
4.6 x 150 mm,5 μm
Mobile phase:
Flow rate:
100% water
1mL/min; the flow was stopped for 5
minutes during the testing period for each column.
Temperature:
30 ℃
Greater Volume Loading Capacity Than Other Brands
Large Volume Injection (Injection volume =100 μL)
Sample:
4-methoxybenzenesulfon amide,
10 μg/mL in MeOH/Water (1:1)
Column:
4.6 x 150 mm, 5μm
Mobile phase:
MeOH/Water
Flow rate:
1mL/min
Temperature:
30 ℃
Stability of Unisol C18 at pH=1.5
Sample: Uracil, Doxepin，Amitriptyline,Toluene.
Column: Unisol C18, 4.6 x 150 mm, 5 μm
Mobile phase: TFA (0.25%, pH 1.5) in 60% acetonitrile
Flow rate: 1 mL/ min.;
Temperature: 40 ℃
Perfect Peak Symmetry and Good Stability at Mid-pH for Basic Compounds
Sample: Uracil, Doxepin, Nortriptyline,Amitriptyline, Trimipramine
Column: Unisol C18, 4.6 x 250 mm, 5 μm
Mobile phase: 0.01 M sodium phosphate: ACN = 25 : 75, pH=7.0
Flow rate: 1.3 mL/min.
Temperature: 30 ℃
Unisol Amide (HILIC)
Unisol Amide stationary phase is made from high purity spherical silica particles bonded with a neutral
hydrophilic amide group. It can be used for normal phase，reverse phase, or hydrophilic interaction HPLC.
The Unisol Amide (NP) column is a good replacement for NH2 and Silica columns that are currently
marketed as HILIC. Compared with traditional silica and NH2 columns, the Unisol Amide columns have
better reproducibility and column lifetime. It is especially useful for the separation of strong hydrophilic
compounds, whether they are acidic, basic or neutral. It is compatible with aqueous mobile phases in the
pH range of 2-8. It has selectivity very different from most of conventional stationary phases
Loading 6%; Single End-cap; Available Particle Size: 3μm, 5μm, 10μm
Main features:
●Strong retention of polar compounds in HILIC mode
●Very unique selectivity and good complementary to the conventional reverse phase
●Can be used as reverse phase, normal phase and HILIC phase
●More robust and reproducible performance than silica and amino stationary phase
●100% aqueous and 100% organic solvents compatible
●pH range (2-8)
Separation of Validamycin
Mobile Phase: A: 0.1% TFA in WaterB: Acetonitrile
Gradient: 40% A to 85% A in 30 min
Flow Rate: 1 mL/min
Temperature: 25℃
Detection:
Sample:
UV 210 nm
Validamycin
Mobile Phase: A: 0.005M NaH2PO4(pH=7) ;
B:MeOH A:B=98:2
Flow Rate:
1 mL/min
Temperature: 25℃
Detection:
Sample:
UV 210 nm
Validamycin
Separation of Shikimic Acid
Column: Unisol Amide, 5 μm, 4.6mm×150mm
Part No.: UA951505-0
Mobile Phase:A: 1% Fomic Acid in Water B: Acetonitrile
Gradient: 60% A to 90% A in 20 min
Flow Rate: 1 mL/min
Temperature: 25℃
Sample: Shikimic Acid
Water-soluble Vitamins
Unisol Amide，5 μm, 4.6mm×150mm
Brand W, NH2 Column, 5 μm, 4.6×150
mm
Brand W Silica Column, 5μm, 4.6×150mm
Mobile Phase:A: 0.1% TFA in Water ；B:
0.1% TFA in Acetonitrile A: B = 90:10
Flow Rate: 1 mL/min
Temperature: 30℃
Sample: VB1+VB6+VC+VB2
Ordering Information
Surface Area: 410 m2/g, Pore Size: 100Å
Dimension
Particle
Unisol
Unisol
(mm)
Size(μm)
C18
Amide(HILIC)
Fast analysis
2.1×30
2.5
UO920302-0
Fast analysis
2.1×50
2.5
UO920502-0
Fast analysis
2.1×100
2.5
UO921002-0
Fast analysis
3.0×30
2.5
UO920303-0
Fast analysis
3.0×50
2.5
UO920503-0
Fast analysis
3.0×100
2.5
UO921003-0
Fast analysis
4.6×50
2.5
UO920505-0
Fast analysis
4.6×100
2.5
UO921005-0
Analytical
2.1×30
5
UO950302-0
VH950302-0
Analytical
2.1×50
5
UO950502-0
VH950502-0
Analytical
2.1×100
5
UO951002-0
VH951002-0
Analytical
2.1×150
5
UO951502-0
VH951502-0
Analytical
3.0×30
5
UO950303-0
VH950303-0
Analytical
3.0×50
5
UO950503-0
VH950503-0
Analytical
3.0×100
5
UO951003-0
VH951003-0
Analytical
4.6×50
5
UO950505-0
VH950505-0
Analytical
4.6×100
5
UO951005-0
VH951005-0
Analytical
4.6×150
5
UO951505-0
VH951505-0
Analytical
4.6×250
5
UO952505-0
VH952505-0
Gaurd cartridge
4.6×10
5
UO950105-0
VH950105-0
Fast analysis
2.1×30
3
UO930302-0
VH930302-0
Fast analysis
2.1×50
3
UO930502-0
VH930502-0
Fast analysis
2.1×100
3
UO931002-0
VH931002-0
Fast analysis
2.1×150
3
UO931502-0
VH931502-0
Fast analysis
3.0×30
3
UO930303-0
VH930303-0
Fast analysis
3.0×50
3
UO930503-0
VH930503-0
Fast analysis
3.0×100
3
UO931003-0
VH931003-0
Fast analysis
4.6×50
3
UO930505-0
VH930505-0
Fast analysis
4.6×100
3
UO931005-0
VH931005-0
Fast analysis
4.6×150
3
UO931505-0
VH931505-0
Gaurd cartridge
4.6×10
3
UO930105-0
VH930105-0
Semi-preparative
10×150
5
UO951510-0
VH951510-0
Semi-preparative
10×250
5
UO952510-0
VH952510-0
Preparative
20×50
5
UO950520-0
VH950520-0
Preparative
20×150
5
UO951520-0
VH951520-0
Preparative
20×250
5
UO952520-0
VH952520-0
Preparative
30×100
5
UO951030-0
VH951030-0
Preparative
30×150
5
UO951530-0
VH951530-0
Preparative
30×250
5
UO952530-0
VH952530-0
Guard cartridge
20×20
5
UO950220-0
VH950220-0
Type
Optimix Technology Column Products
NEW in HPLC !
Conventional silica based HPLC stationary phases are usually bonded with mono-type alkyl chain
molecules, e.g., C18, C8, CN, etc. Recent studies revealed that synergistic effects can be obtained when
the bonded surface consists of mixed phases of C18 and a shorter chain (C8, amide, CN, nitro-phenyl,
etc). These new products offer different selectivity, coordinated multi-functionality, as well as a spacer
effect (C18/C8).
It is difficult to produce a uniformly bonded C18 surface on silica due to steric hindrance of the bulky C18
chain. This problem can be easily overcome when the surface is bonded with mixed chain lengths, C18
and a shorter silane. As a result, there are many advantages: improved surface uniformity, better
penetration of molecules during partitioning due to less steric hindrance, and different selectivity
compared to a mono-chain type stationary phase.
As one of the leaders in separation technologies, Agela Technologies has innovatively developed a
family of non-ionic, mixed-phase HPLC/SFC columns.
Consider these columns as options for your HPLC method development
Optimix C18/C8:
Improved performance from a mixed-phase C18/C8 column compared to mono-chain C18 or C8
columns
●Alternative selectivity
●Improved selectivity for stereo isomers, better differentiation of shapes
●Better peak shape due to uniformity of the bonding.
●Balanced hydrophilicity and hydrophobicity: extended retention for polar compounds; non-excessive
retention for strong hydrophobic compounds
Characteristics: Metal Impurity < 10 ppm; Pore Size: 100 Å; Specific Surface Area: 410 m2/g;
Carbon Loading: 16%; Double End-cap; Available Particle Size: 3μm, 5μm, 10μm.
Analysis of Vancomycin and its impurity
Column:
Optimix C18 /C8,5μm 4.6×250mm，
Part No.:
OP952505-OC
Mobile Phase: A: 0.7% TEA and 3% Acetic Acid inWater
B: Methanol ;C:THF ;A:B:C(88:10:2)
Flow Rate:
1 mL/min
Temperature:
25℃
Detection:
UV 210 nm
Sample:
Vancomycin＋Impurity
Column:
Brand W C18 Column,5μm , 4.6×250mm，
Mobile Phase: USP 24
A: 0.2% TEA (pH3.2)-Acetonitrile-THF(92:7:1)
B: 0.2% TEA (pH3.2)-Acetonitrile-THF(70:29:1)
Gradient：
USP 24
Time(min)
0
12
20
22
23
30
B%
0
0
100
100
0
0
Optimix C18/Amide
A mixed-phase of C18 and propionic amide provides unique selectivity which allows extremely polar and
non-polar compounds to be analyzed in a single run under isocratic conditions.
Characteristics: Metal Impurity < 10 ppm; Pore Size: 100 Å; Specific Surface Area : 410 m2/g; Carbon
Loading : 11%; Single End-cap; Available Particle Size: 3 μm, 5 μm
Performance Features:
●Unique selectivity
●Balanced retention for extremely polar and non-polar compounds
●Greater retention for polar compounds due to coordinated effects of C18 and amide
●Minimal tailing for basic compounds compared to any other type of columns, due to suppression of
silanol effects by the amide group.
Bring extremely polar and non-polar analytes closer to shorten the analysis time
under isocratic conditions
Mobile Phase：A: Water, B: Methanol
Sample:
Uracil, Toluene
Flow rate:
1 mL/min
Detection:
UV = 254 nm
t0= 1.52 min
Column: Optimix C18/amide,5μm, 4.6×150mm
Column: C18, 5μm,4.6×150mm
Mobile Phase: A : B = 50 : 5
Mobile Phase: A : B = 60 : 40
Surface Area: 410 m2/g, Pore Size: 100Å
Dimension
Particle
Optimix
Optimix
Optimix
Optimix
(mm)
size(μm)
C18/C8
C18/amide
C18/phenyl
C18/CN
Analytical
2.1×30
5
OP950302-OC
OP950302-AM
OP950302-PH
OP950302-CN
Analytical
2.1×50
5
OP950502-OC
OP950502-AM
OP950502-PH
OP950502-CN
Analytical
2.1×100
5
OP951002-OC
OP951002-AM
OP951002-PH
OP951002-CN
Analytical
2.1×150
5
OP951502-OC
OP951502-AM
OP951502-PH
OP951502-CN
Analytical
4.6×50
5
OP950505-OC
OP950505-AM
OP950505-PH
OP950505-CN
Analytical
4.6×100
5
OP951005-OC
OP951005-AM
OP951005-PH
OP951005-CN
Analytical
4.6×150
5
OP951505-OC
OP951505-AM
OP951505-PH
OP951505-CN
Analytical
4.6×200
5
OP952005-OC
OP952005-AM
OP952005-PH
OP952005-CN
Analytical
4.6×250
5
OP952505-OC
OP952505-AM
OP952505-PH
OP952505-CN
4.6×10
5
OP950105-OC
OP950105-AM
OP950105-PH
OP950105-CN
Fast analysis
2.1×30
3
OP930302-OC
OP930302-AM
OP930302-PH
OP930302-CN
Fast analysis
2.1×50
3
OP930502-OC
OP930502-AM
OP930502-PH
OP930502-CN
Fast analysis
2.1×100
3
OP931002-OC
OP931002-AM
OP931002-PH
OP931002-CN
Fast analysis
2.1×150
3
OP931502-OC
OP931502-AM
OP931502-PH
OP931502-CN
Fast analysis
4.6×50
3
OP930505-OC
OP930505-AM
OP930505-PH
OP930505-CN
Fast analysis
4.6×100
3
OP931005-OC
OP931005-AM
OP931005-PH
OP931005-CN
Fast analysis
4.6×150
3
OP931505-OC
OP931505-AM
OP931505-PH
OP931505-CN
4.6×10
3
OP930105-OC
OP930105-AM
OP930105-PH
OP930105-CN
Type
Guard
cartridge
Guard
cartridge
Dimension
Particle
Optimix
Optimix
Optimix
(mm)
size(μm)
C18/SCX
C18/SAX
C18/Nitrophenyl
Analytical
2.1×30
5
OP950302-SC
OP950302-SA
OP950302-NP
Analytical
2.1×50
5
OP950502-SC
OP950502-SA
OP950502-NP
Analytical
2.1×100
5
OP951002-SC
OP951002-SA
OP951002-NP
Analytical
2.1×150
5
OP951502-SC
OP951502-SA
OP951502-NP
Analytical
4.6×50
5
OP950505-SC
OP950505-SA
OP950505-NP
Analytical
4.6×100
5
OP951005-SC
OP951005-SA
OP951005-NP
Analytical
4.6×150
5
OP951505-SC
OP951505-SA
OP951505-NP
Analytical
4.6×200
5
OP952005-SC
OP952005-SA
OP952005-NP
Analytical
4.6×250
5
OP952505-SC
OP952505-SA
OP952505-NP
4.6×10
5
OP950105-SC
OP950105-SA
OP950105-NP
Fast analysis
2.1×30
3
OP930302-SC
OP930302-SA
OP930302-NP
Fast analysis
2.1×50
3
OP930502-SC
OP930502-SA
OP930502-NP
Fast analysis
2.1×100
3
OP931002-SC
OP931002-SA
OP931002-NP
Fast analysis
2.1×150
3
OP931502-SC
OP931502-SA
OP931502-NP
Fast analysis
4.6×50
3
OP930505-SC
OP930505-SA
OP930505-NP
Fast analysis
4.6×100
3
OP931005-SC
OP931005-SA
OP931005-NP
Fast analysis
4.6×150
3
OP931505-SC
OP931505-SA
OP931505-NP
4.6×10
3
OP930105-SC
OP930105-SA
OP930105-NP
Type
Guard
cartridge
Guard
cartridge
Durashell Technology and Column Products NEW in HPLC !
Agela’ s Durashell column has a pH range of 1-12. Different from the current marketed products that also
can be used at high pH,Durashell’ s stationary phase is made with a dentrimer technology followed by
molecular modifications. Agela’ s technology produces a strong hydrophobic protection layer over the
silica surface allowing the columns to be used at extremely high and low pH. This technology also
reduces excessive hydrophobic interactions between the stationary phase and the compounds, and yet
maintains great interfacial kinetics for high efficiency.
Loading 15%; Double End-cap; Available Particle Size: 5μm, 10 μm
Main Features:
●Wide pH range (1.5-12)
●Minimal silanol activity
●Better efficiency than many other high pH compatible columns
●High loading capacity for basic compounds for preparative applications
High pH Stability
Column:
Durashell RP; 4.6 x 150 mm
Mobile Phase: 50% ACN/50% 0.05M Ammonia
(pH=9.0)
Flow rate:
1.5 mL/min
Sample:
doxepin, nortriptyline, amitriptyline,
trimipramine ,
Temperature: 35℃
Surface Area: 240m2/g, Pore Size: 150Å
Particle
Dimension
Size(μm)
(mm)
Analytical
5
2.1×30
DS950302-0
Analytical
5
2.1×50
DS950502-0
Analytical
5
2.1×100
DS951002-0
Analytical
5
2.1×150
DS951502-0
Analytical
5
4.6×50
DS950505-0
Analytical
5
4.6×100
DS951005-0
Analytical
5
4.6×150
DS951505-0
Analytical
5
4.6×200
DS952005-0
Analytical
5
4.6×250
DS952505-0
Guard cartridge
5
4.6×10
DS950105-0
Fast analysis
3
2.1×30
DS930302-0
Fast analysis
3
2.1×50
DS930502-0
Fast analysis
3
2.1×100
DS931002-0
Fast analysis
3
2.1×150
DS931502-0
Fast analysis
3
4.6×50
DS930505-0
Fast analysis
3
4.6×100
DS931005-0
Fast analysis
3
4.6×150
DS931505-0
Guard cartridge
3
4.6×10
DS930105-0
Semi-preparative
5
10×150
DS951510-0
Semi-preparative
5
10×250
DS952510-0
Preparative
5
20×50
DS950520-0
Preparative
5
20×150
DS951520-0
Preparative
5
20×250
DS952520-0
Preparative
5
30×100
DS951030-0
Preparative
5
30×150
DS951530-0
Preparative
5
30×250
DS952530-0
Guard cartridge
5
20×20
DS950220-0
Semi-preparative
10
10×150
DS901510-0
Semi-preparative
10
10×250
DS902510-0
Preparative
10
20×50
DS900520-0
Preparative
10
20×150
DS901520-0
Preparative
10
20×250
DS902520-0
Preparative
10
30×100
DS901030-0
Preparative
10
30×150
DS901530-0
Preparative
10
30×250
DS902530-0
Preparative
10
50×150
DS901550-0
Preparative
10
50×250
DS902550-0
Guard cartridge
10
20×20
DS900220-0
Type
Durashell RP
Venusil Family of HPLC Columns Full Line of High Quality Products
Introduction of Venusil HPLC Columns
Venusil HPLC columns are manufactured from the highest purity spherical silica particles on the market.
The outstanding properties of the HPLC phases are results of subjecting high quality silica to Agela’ s
innovative surface modification and unique bonding processes. Our proprietary nanosurface modification
generates a very smooth and even surface. This reduces the interaction between the silica surface and
polar compounds, resulting in symmetric peak shape even for very basic compounds. Based on this
technology, a series of unique columns were developed to meet the tough requirements for the analysis
of highly polar compounds. Lot-to-lot reproducibility is ensured by a stable bonding/end-capping process.
The uniform, spherical particles have a nominal surface area of 380m2/g, 200 m2/g or 45m2/g, with a
controlled pore size of 100Å, 150Å or 300Å, respectively. All columns are packed using a consistent
slurry packing process to achieve uniform and stable beds for maximum column efficiency, lifetime and
column-to-column reproducibility.
Unprecedented Experience in Chromatography
You will experience unprecedented performance using Venusil columns for your HPLC separations
Best Peak Symmetry and Efficiency
The Venusil columns generate symmetric peaks with high efficiency over their entire applicable pH
range,outperforming competitors columns.
The pH-independent high performance feature of Venusil columns allows scientists to establish rugged
methods with flexible choice of pH.
Tightest Specification
All our columns have tighter specification (see the table) than other brand-name columns. This ensures
that all columns have great performance and identical selectivity
Comparison of Specifications
Waters Symmetry-C18
Zorbax XDB-C18
Venusil XBP C18 (2)
4.6×150mm, 5μm
4.6×150mm ,5μm
4.6×150mm ,5μm
Spec. on efficiency
N/A
8000
12000
Spec. on tailing factor(toluene)
N/A
0.98-1.25
0.98-1.12
Spec. on tailing factor-amitriptyline
1.8
Spec. on
N/A(typically
1.5-2.0)
1.4
+/- 10%
N/A
+/- 7%
pH range
2-8
2-9
1.5-9
Specific Surface
300
180
380
Carbon%
16%
12%
19%
selectivity(amitriptyline/acenaphthene)
Characteristics of Venusil Columns
Benefit to the Columns’ users
Perfect peak symmetry, independent of pH
Highest flexibility for any application,better sensitivity,
and mobile phase
greater confidence on HPLC results
Broad pH range (1-10.5)
Flexibility for method development
Compatible with 100% aqueous mobile phase
Maximum versatility
Highest surface coverage
Tolerance of large volume and mass injection
A wide variety of chemistries
Broad selection of polarity and maximum versatility
Minimum buffer concentration needed
Better sensitivity for LC/MS, extended column lifetime
Tightest specification
Better reproducibility, greater confidence on the HPLC results
Excellent column efficiency
Better and faster separation
Great column lifetime
Lower cost; greater convenience
Expanding the Capabilities with the Venusil Family of C18 Columns
Agela Technologies has developed a series of high quality C18 stationary phases to meet a wide range
of application needs.These columns contain an ultra pure silica subjected to our patented surface
deactivation process. By altering the column chemistry, we are able to tune the surface properties of the
silica particles and alter selectivity to meet a variety of application needs.
Many of our columns have very unique properties to address some special needs. Here are some
examples:
Venusil XBP-C18 (2): a phase designed for balanced adsorption to avoid excessive retention of
hydrophobic compounds,
• Great peak symmetry for all types of compounds,
• Improved separation of stereo isomers
• Extremely narrow specification during manufacture to offer high column-to-column reproducibility
• Non-excessive retention for hydrophobic peaks (less peak broadening of later eluting compounds
compared to other columns)
Venusil ASB C18: a phase designed for low pH, low bleed (high sensitivity for LC-MS) and strong
separation power for polar compounds:
• Extremely low pH stability: pH limit =0.8 at 70 ℃
• Extremely low bleed offering high sensitivity for LC-MS under acidic conditions
• Compatible with 100% aqueous to 100% organic mobile phases
• Non-endcapped but with low surface acidity/activity compared to other non-endcapped stationary
phases
Venusil AQ C18: a phase designed for polar and semi-polar compounds, to be compatible with 100%
water :
• Compatible with 100% aqueous to 100% organic mobile phases
• Applicable to a variety of analytes: from very polar to non-polar
• Operates over a wide pH range: 1.0-9.0
• Applicable to a wide range of sample types: plasma, urine, drug formulation, food extraction
• Available in a range of column diameters suitable for LC-MS, conventional analytical, and preparative
scale
Venusil XBP-C18: a phase designed for maximum hydrophobicity and high pH tolerance:
• The highest carbon loading and the most hydrophobic column on the market.
• High pH tolerance
• Not suggested for samples containing highly hydrophobic compounds
Venusil XBP-C18 (L): a phase designed for larger molecules and highly hydrophobic compounds:
• Larger pore size and lower surface area
• Accelerated elution for highly hydrophobic compounds
• Easier column clean-up for samples containing hydrophobic impurities or samples extracted by
non-polar solvents
• Better choice for molecules > 500 Dalton
Venusil XBP C18 (2) Series Columns
Venusil XBP C18 (2) column packing material is made with ultra pure silica. The silica surface is
processed with Agela’ s patented surface deactivation technology, followed by a unique bonding
process that can reduce the carbon content while maintaining a high bonding coverage. The Venusil XBP
C18 column does not have excessive retention for highly hydrophobic compounds, and it is great for the
separation of acidic, basic, and neutral compounds. Moreover, this column also has superb resolution
power for isomers. The perfect peak symmetry offered by this RP column makes it a great first-choice for
HPLC method development.
Universal, Highly Inert C18 Columns
z
Balanced hydrophobic adsorption which results in a broader suitability
z
Stronger separation power for isomers
z
Great column-to-column and batch-to-batch reproducibility
z
Perfect symmetry for basic, acidic and neutral compounds
A good replacement of Luna C18 (2) columns!
Technical Parameters
z
Metal Impuriy < 10ppm
z
Particle Size: 5μm, 3μm, 10μm
z
Specific Surface: 380m2/g
z
Pore Size: 100Å
z
Carbon Loading: 19%
z
Double End-cap
z
pH Range: 1.5-9.0
z
Performance: Efficiency＞80,000/m (5μm)
z
TF: 0.98-1.12
Hydrophobicity Test
Column:
Sample:
Venusil XBP C18 (2), 4.6 × 150mm, 5 μm
Uracil, Toluene
Mobile phase: MeOH: 25 mM KH2PO4/K2HPO4 (pH 6.0) =
80 : 20
Inertness Test
Detection:
UV 254 nm
Flow rate:
1mL/min
Column:
Sample:
Venusil XBP C18 (2), 4.6 × 150mm, 5 μm
Amitriptyline
Mobile phase: NaH2PO4/Na2HPO4 （ 20mM, pH 6.9）:
MeOH = 29 : 71
Detection:
UV 254 nm
Flow rate:
1mL/min
Amitriptyline is a strong basic antidepressant. It is recognized
as a compound difficult to obtain good peak shape; But the
XBP C18（2）column performed outstandingly and gave a great
peak symmetry （TF = 1.12）.
Extremely Low Metal Effects
Selectivity of Basic Compounds
Mobile Phase: Water :Methanol=65:35
Mobile Phase： MeOH：Acetic Acid/ sodium acetate
Flow Rate:
aq.
1 mL/min
Temperature: 35℃
(pH 5.0）=60：40
Detection:
UV 230 nm
Flow Rate：
Sample:
2,3-Dihydroxynaphthalene＋2,7
-Dihydroxynaphthalene
Detection：
1 mL/min
UV 254 nm
Surface Area: 380m2/g,Pore Size: 100Å
Type
Dimension (mm)
Particle size(μm)
Venusil XBP-C18 (2)
Analytical
2.1×30
5
VX950302-2
Analytical
2.1×50
5
VX950502-2
Analytical
2.1×100
5
VX951002-2
Analytical
2.1×150
5
VX951502-2
Analytical
4.6×50
5
VX950505-2
Analytical
4.6×100
5
VX951005-2
Analytical
4.6×150
5
VX951505-2
Analytical
4.6×250
5
VX952505-2
Guard cartridge
4.6×10 4/pk
5
VX950105-2
Venusil XBP Series Columns
Venusil XBP C18
Venusil XBP C18 columns have the maximum bonding density therefore the highest hydrophobicity (the
lowest polarity). This allows for the least interaction between the analytes and the silanol groups. Venusil
XBP columns have extraordinary column stability at high pHs.
Pore size: 100Å, 300 Å; Specific surface: 380 m2/g,45m2/g; Purity > 99.999%;
1) Inertness: one of most base-friendly columns.
2) Efficiency: excellent at any applicable pH.
3) Large volume injection: maintain very high efficiency with very large injection volumes.
4) Broad pH range (1.5 – 10.0 ): low pH, 0.2 % TFA; high pH,0.010 M triethylamine.
Other Venusil XBP Columns: A complete line of stationaryphases include C8, C4, C1, NH2, Phenyl, CN,
Silica, SCX,SAX;
Quality of Column Packing
Perfect Peak Symmetry and Great Lifetime of
the Column for Basic Compounds at Mid-pH
Sample:
uracil, phenol,chloronitrobenzene,
Sample:
toluene(in methanol/water=50/50)
Column:
2.1 x 50 mm, 5 μm
Uracil, Doxepin,Nortriptyline,Amitriptyline,
Trimipramine
Column:
Venusil XBP-C18, 4.6 x 150 mm, 5 μm
Mobile phase: methanol: water = 65: 35
Mobile phase:
Flow rate:
Flow rate:
1.5 mL/min
Temperature:
45 ℃
0.20 mL/min
Temperature: 30 ℃
0.01 M sodium phosphate: ACN= 50 : 50, pH=7.6
Comparison of Venusil-C8 Phases
Plates (Toluene)
Tailing Factor
Brand Z-C8
3805
1.12
Luna C8
2546
1.15
Venusil XBP-C8
4012
1.03
Dimension
Particle
Venusil
Venusil
Venusil
Venusil
(mm)
(μm)
XBP-C18
XBP-C8
XBP-C4
XBP-C1
Analytical
2.1×30
5
VX950302-0
VX850302-0
VX450302-0
VX150302-0
Analytical
2.1×50
5
VX950502-0
VX850502-0
VX450502-0
VX150502-0
Analytical
2.1×100
5
VX951002-0
VX851002-0
VX451002-0
VX151002-0
Analytical
2.1×150
5
VX951502-0
VX851502-0
VX451502-0
VX151502-0
Analytical
4.6×50
5
VX950505-0
VX850505-0
VX450505-0
VX150505-0
Analytical
4.6×100
5
VX951005-0
VX851005-0
VX451005-0
VX151005-0
Analytical
4.6×150
5
VX951505-0
VX851505-0
VX451505-0
VX151505-0
Analytical
4.6×200
5
VX952005-0
VX852005-0
VX452005-0
VX152005-0
Analytical
4.6×250
5
VX952505-0
VX852505-0
VX452505-0
VX152505-0
Guard cartridge
4.6×10 4/pk
5
VX950105-0
VX850105-0
VX450105-0
VX150105-0
Fast analysis
2.1×30
3
VX930302-0
VX830302-0
Fast analysis
2.1×50
3
VX930502-0
VX830502-0
Fast analysis
2.1×100
3
VX931002-0
VX831002-0
Fast analysis
2.1×150
3
VX931502-0
VX831502-0
Fast analysis
4.6×50
3
VX930505-0
VX830505-0
Fast analysis
4.6×100
3
VX931005-0
VX831005-0
Fast analysis
4.6×150
3
VX931505-0
VX831505-0
Guard cartridge
4.6×10 4/pk
3
VX930105-0
VX830105-0
Semi-preparative
10×150
5
VX951510-0
VX851510-0
Semi-preparative
10×250
5
VX952510-0
VX852510-0
Preparative
20×50
5
VX950520-0
VX850520-0
Preparative
20×150
5
VX951520-0
VX851520-0
Preparative
20×250
5
VX952520-0
VX852520-0
Preparative
30×100
5
VX951030-0
VX851030-0
Preparative
30×150
5
VX951530-0
VX851530-0
Preparative
30×250
5
VX952530-0
VX852530-0
Guard cartridge
20×10
5
VX950120-0
VX850120-0
Semi-preparative
10×150
10
VX901510-0
VX801510-0
Semi-preparative
10×250
10
VX902510-0
VX802510-0
Preparative
20×50
10
VX900520-0
VX800520-0
Preparative
20×150
10
VX901520-0
VX801520-0
Preparative
20×250
10
VX902520-0
VX802520-0
Preparative
30×100
10
VX901030-0
VX801030-0
Preparative
30×150
10
VX901530-0
VX801530-0
Type
Preparative
30×250
10
VX902530-0
VX802530-0
Preparative
50×150
10
VX901550-0
VX801550-0
Preparative
50×250
10
VX902550-0
VX802550-0
Guard cartridge
20×10
10
VX900120-0
VX800120-0
Dimension
Particle
Venusil
Venusil
Venusil
Venusil
(mm)
(μm)
XBP-NH2
XBP-Phenyl
XBP-CN
Type Silica
Analytical
2.1×30
5
VN850302-0
VX650302-0
VC950302-0
VSi950302-0
Analytical
2.1×50
5
VN850502-0
VX650502-0
VC950502-0
VSi950502-0
Analytical
2.1×100
5
VN851002-0
VX651002-0
VC951002-0
VSi951002-0
Analytical
2.1×150
5
VN851502-0
VX651502-0
VC951502-0
VSi951502-0
Analytical
4.6×50
5
VN850505-0
VX650505-0
VC950505-0
VSi950505-0
Analytical
4.6×100
5
VN851005-0
VX651005-0
VC951005-0
VSi951005-0
Analytical
4.6×150
5
VN851505-0
VX651505-0
VC951505-0
VSi951505-0
Analytical
4.6×200
5
VN852005-0
VX652005-0
VC952005-0
VSi952005-0
Analytical
4.6×250
5
VN852505-0
VX652505-0
VC952505-0
VSi952505-0
4.6×10 4/pk
5
VN850105-0
VX650105-0
VC950105-0
VSi950105-0
Type
Guard
cartridge
Dimension
Particle
Venusil
Venusil
Venusil
Venusil
(mm)
(μm)
XBP-C18
XBP-C8
XBP-C4
XBP-C1
Analytical
2.1×30
5
VX950302-T
VX850302-T
VX450302-T
VX150302-T
Analytical
2.1×50
5
VX950502-T
VX850502-T
VX450502-T
VX150502-T
Analytical
2.1×100
5
VX951002-T
VX851002-T
VX451002-T
VX151002-T
Analytical
2.1×150
5
VX951502-T
VX851502-T
VX451502-T
VX151502-T
Analytical
4.6×50
5
VX950505-T
VX850505-T
VX450505-T
VX150505-T
Analytical
4.6×100
5
VX951005-T
VX851005-T
VX451005-T
VX151005-T
Analytical
4.6×150
5
VX951505-T
VX851505-T
VX451505-T
VX151505-T
Analytical
4.6×200
5
VX952005-T
VX852005-T
VX452005-T
VX152005-T
Analytical
4.6×250
5
VX952505-T
VX852505-T
VX452505-T
VX152505-T
Guard cartridge
4.6×10 4/pk
5
VX950105-T
VX850105-T
VX450105-T
VX150105-T
Semi-preparative
10×150
5
VX951510-T
VX851510-T
Semi-preparative
10×250
5
VX952510-T
VX852510-T
Preparative
20×50
5
VX950520-T
VX850520-T
Preparative
20×150
5
VX951520-T
VX851520-T
Preparative
20×250
5
VX952520-T
VX852520-T
Guard cartridge
20×10
5
VX950120-T
VX850120-T
Dimension
Particle
Venusil
Venusil
Venusil
Venusil
(mm)
(μm)
XBP-NH2
XBP-Phenyl
XBP-CN
Type Silica
Type
2
Surface Area: 45m /g,Pore Size: 300Å
Type
Analytical
2.1×30
5
VN850302-T
VX650302-T
VC950302-T
VSi950302-T
Analytical
2.1×50
5
VN850502-T
VX650502-T
VC950502-T
VSi950502-T
Analytical
2.1×100
5
VN851002-T
VX651002-T
VC951002-T
VSi951002-T
Analytical
2.1×150
5
VN851502-T
VX651502-T
VC951502-T
VSi951502-T
Analytical
4.6×50
5
VN850505-T
VX650505-T
VC950505-T
VSi950505-T
Analytical
4.6×100
5
VN851005-T
VX651005-T
VC951005-T
VSi951005-T
Analytical
4.6×150
5
VN851505-T
VX651505-T
VC951505-T
VSi951505-T
Analytical
4.6×200
5
VN852005-T
VX652005-T
VC952005-T
VSi952005-T
Analytical
4.6×250
5
VN852505-T
VX652505-T
VC952505-T
VSi952505-T
Guard cartridge
4.6×10 4/pk
5
VN850105-T
VX650105-T
VC950105-T
VSi950105-T
Venusil XBP (L) Series Columns
XBP (L) C18 has an identical bonded phase as XBP C18. However, XBP (L) C18 has relatively low surface
area which allows compounds to have shorter retention times. These columns are an excellent alternative to
Hypersil BDS-C18, Hypersil BDS-C8; Zorbax XDB-C18, Zorbax XDB-C8 which comparitively could have too
much retention.
XBP (L) C18 have relatively larger pore size (150Å) and are suitable for the separation of large molecules.
Dimension
Particle
Venusil
Venusil
Venusil
(mm)
(μm)
XBP(L) C18
XBP(L) C8
XBP(L) Silica
Analytical
2.1×30
5
VX950302-L
VX850302-L
VSi950302-L
Analytical
2.1×50
5
VX950502-L
VX850502-L
VSi950502-L
Analytical
2.1×100
5
VX951002-L
VX851002-L
VSi951002-L
Analytical
2.1×150
5
VX951502-L
VX851502-L
VSi951502-L
Analytical
4.6×50
5
VX950505-L
VX850505-L
VSi950505-L
Analytical
4.6×100
5
VX951005-L
VX851005-L
VSi951005-L
Analytical
4.6×150
5
VX951505-L
VX851505-L
VSi951505-L
Analytical
4.6×250
5
VX952505-L
VX852505-L
VSi952505-L
Type
StationaryPhase
Specific
Pore
SurfaceArea
Size
2
(Å)
(m /g)
Particle
Carbon
Size
pH
Loading
Available
Range
Characteristics
(μm)
Balanced adsorption to avoid excessive
reten-tion; a wider range of suitability;
Venusil XBP-C18(2)
380
100
19%
3, 5, 10
1.5
perfect peak symmetry for acidic, basic
and neutral com-pounds; greater
separation power for isomers.
The most hydrophobic adsorption; better
Venusil XBP-C18
380
100
22%
3, 5, 10
1.5
high pH tolerance; not suggested for
samples which contain highly
hydrophobic compounds.
Larger pore size and lower surface area;a
Venusil XBP-C18(L)
200
150
15%
3, 5, 10
1.5
better choice for the analysis of large
molecule and strong hydrophobic
compounds.
Slightly polar C18 and enhanced
Venusil AQ C18
380
100
18%
3, 5, 10
1
retention for polar compounds; 100%
water compatible; good peak shape for
acidic, basic and neutral compounds.
Polar C18; extremely low bleed at low
Venusil ASB-C18
200
150
12%
3, 5, 10
0.8
pH; great
for LC-MS and peptide
separation; stable at pH as low as 0.8 at
70℃.
Venusil AQ-C18 Columns
The Venusil AQ-C18 column is designed for the separation of polar, semi-polar and non-polar compounds
at low to medium pH. This column is more polar than XBP-C18, but less polar than ASB-C18. With a special
surface treatment, Venusil AQ-C18 is made to be compatible with 100% aqueous mobile phases., and it can
be used to replace Waters Symmetry, Inertsil C18, Zorbax SB, Atlantis dC18, and other AQ columns. This
column has a pH range of 1.5-9.0.
Pore size: 100 Å; Specific surface: 380 m2/g; Purity > 99.999%;
1) Inertness: one of most base-friendly columns, excellent peak symmetry of basic compounds.
2) Efficiency: The best column for polar compounds at any pH.
3) Large volume injection: maintains very high efficiency even if the injection volume is exceptionally large.
4) 100% water compatible: very unique for a universal C18 column; much better peak shape, retention, and
efficiency than other brand AQ columns.
5) Low pH stability (pH=1.5):better stability than most of the popular brand columns (including other brand
AQ columns) on the market.
Compatibility with 100% Aqueous Mobile Phase
Sample:
Ranitidine
Column:
4.6×150mm,5μm
Mobile phase:
100% Water
Flow rate:
1mL/min; the flow was stopped for 5 minutes
during the testing period for each column.
Temperature: 30℃
Balanced Retention for Hydrophilic and Hydrophobic Compounds
Mobile Phase:
35% v/v 20.0 mM KH2PO4/K2HPO4
at pH7.00/65% Methanol at 23℃
Flow:
1mL/min
Detection:
UV 254nm
Sample:
Uracil Propranolol hydrochloride
Butyl ,Phthalate Dimethyl Phthalate
Naphthalene ,Acenaphthene Amitriptyline
Great Peak Shape for All Type of Compounds
Mobile Phase：1% Acetic Acid (pH=2.57) :MeOH =50:50
Flow ：
1mL/min
Detection：
Sample：
275nm
Uracil、Paracetamol、acetophenone and
2-Hydroxybenzoic acid
Type
Dimension
Particle
Venusil
(mm)
(μm)
AQ-C18
Analytical
2.1×30
5
VA950302-0
Analytical
2.1×50
5
VA950502-0
Analytical
2.1×100
5
VA951002-0
Analytical
2.1×150
5
VA951502-0
Analytical
4.6×50
5
VA950505-0
Analytical
4.6×100
5
VA951005-0
Analytical
4.6×150
5
VA951505-0
Analytical
4.6×250
5
VA952505-0
Guard cartridge
4.6×10 4/pk
5
VA950105-0
Fast analysis
2.1×30
3
VA930302-3
Fast analysis
2.1×50
3
VA930502-0
Fast analysis
2.1×100
3
VA931002-0
Fast analysis
2.1×150
3
VA931502-0
Fast analysis
4.6×50
3
VA930505-0
Fast analysis
4.6×100
3
VA931005-0
Fast analysis
4.6×150
3
VA931505-0
Guard cartridge
4.6×10 4/pk
3
VA930105-0
Semi-preparative
10×150
5
VA951510-0
Semi-preparative
10×250
5
VA952510-0
Preparative
20×50
5
VA950520-0
Preparative
20×150
5
VA951520-0
Preparative
20×250
5
VA952520-0
Preparative
30×100
5
VA951030-0
Preparative
30×150
5
VA951530-0
Preparative
30×250
5
VA952530-0
Guard cartridge
20×10
5
VA950120-0
Venusil ASB Columns
The Venusil ASB column is specially designed for the separation of polar compounds from low (extremely
stable at pH=1) to medium pH. The stationary phase is end-capped with polar groups and thus has the
highest polarity among all Venusil family columns. This column can be used to replace Water Atlantis,
Zorbax SB, and other brand AQ columns.
Pore size: 150Å; Specific surface: 200m2/g; Purity＞99.999%
1) Low pH stability: great stability even at pH=1
2) Peak shape and efficiency: Excellent peak symmetry for basic compounds comparing to other brand
polar phase columns
3) Polar C18 phase: Very strong separation power for polar compounds
4) 100% aqueous compatible: Much better peak shape, retention, and efficiency comparing to other brand
“AQ” columns.
The Stability in the Low pH
Column:
Venusil ASB C18, 5μm, 4.6×150mm
Sample:
Naphthol
Aging:
40 ℃, TFA in 80% methanol
(pH=1.0), 400 hours
Mobile Phase:TFA in 80% methanol (pH=1.5)
Flow Rate:
1 mL/min
Injection:
5 μL
Temperature: 30℃
The separation of Organic Acid
Column:
Venusil ASB C18, 5μm, 4.6×150mm
Sample:
Vc, malonic acid, lactic acid, citric acid
Mobile Phase:20 mM Phosphate buffer saline (PBS) ,
pH=2.0
Flow Rate:
1 mL/min
Injection:
5 μL
Detection:
UV 210 nm
Surface: 200m2/g,Pore size: 150Å
Dimension
Particle
Venusil
Venusil
Venusil
(mm)
(μm)
ASB-C18
ASB-C8
ASB-Phenyl
Analytical
2.1×30
5
VS950302-0
VS850302-0
VS650302-0
Analytical
2.1×50
5
VS950502-0
VS850502-0
VS650502-0
Analytical
2.1×100
5
VS951002-0
VS851002-0
VS651002-0
Analytical
2.1×150
5
VS951502-0
VS851502-0
VS651502-0
Analytical
4.6×50
5
VS950505-0
VS950505-0
VS650505-0
Analytical
4.6×100
5
VS951005-0
VS851005-0
VS651005-0
Analytical
4.6×150
5
VS951505-0
VS851505-0
VS651505-0
Analytical
4.6×250
5
VS952505-0
VS852505-0
VS652505-0
Guard cartridge
4.6×10 4/pk
5
VS950105-0
VS850105-0
VS650105-0
Semi-preparative
10×150
5
VS951510-0
VS851510-0
Semi-preparative
10×250
5
VS952510-0
VS852510-0
Preparative
20×50
5
VS950520-0
VS850520-0
Preparative
20×150
5
VS951520-0
VS851520-0
Preparative
20×250
5
VS952520-0
VS852520-0
Guard cartridge
20×10
5
VS950120-0
VS850120-0
Type
2
Surface Aera: 45m /g,Pore Size: 300Å
Dimension
Particle
Venusil
Venusil
(mm)
(μm)
ASB-C18
ASB-C8
Analytical
2.1×30
5
VS950302-T
VS850302-T
Analytical
2.1×50
5
VS950502-T
VS850502-T
Analytical
2.1×100
5
VS951002-T
VS851002-T
Analytical
2.1×150
5
VS951502-T
VS851502-T
Analytical
4.6×50
5
VS950505-T
VS850505-T
Analytical
4.6×100
5
VS951005-T
VS851005-T
Analytical
4.6×150
5
VS951505-T
VS851505-T
Analytica
4.6×250
5
VS952505-T
VS852505-T
Guard cartridge
4.6×10 4/pk
5
VS950105-T
VS850105-T
Type
Venusil SCX HPLC Columns
SCX HPLC Column: made of spherical silica particles of the highest purity (>99.999%), bonded with
aromatic sulfonic acid group.They can be used forthe separation of basic, water-soluble compounds and
bio-molecules.
Surface Aera: 200m2/g,Pore Size: 150Å
Type
Dimension (mm)
Particle (μm)
Venusil SCX
Analytical
4.6×100
5
VSc951005-0
Analytical
4.6×150
5
VSc951505-0
Analytical
4.6×250
5
VSc952505-0
Guard cartridge
4.6×10 4/pk
5
VSc950105-0
2
Type
Dimension
(mm)
Particle (μm)
Venusil
SCX
Analytical
4.6×100
5
VSc951005-T
Analytical
4.6×150
5
VSc951505-T
Analytical
4.6×250
5
VSc952505-T
Guard cartridge
4.6×10 4/pk
5
VSc950105-T
Venusil SAX HPLC Columns
SAX HPLC Column: the stationary phase is made with high purity spherical silica particles and bonded with
quaternary amine groups.The column can be used for the separation of acidic, water-soluble compounds
and bio-molecules
Type
Dimension
(mm)
Particle (μm)
Venusil
SAX
Analytical
4.6×100
5
VSa951005-0
Analytical
4.6×150
5
VSa951505-0
Analytical
4.6×250
5
VSa952505-0
Guard cartridge
4.6×10 4/pk
5
VSa950105-0
2
Type
Dimension
(mm)
Particle (μm)
Venusil
SAX
Analytical
4.6×100
5
VSa951005-T
Analytical
4.6×150
5
VSa951505-T
Analytical
4.6×250
5
VSa952505-T
Guard cartridge
4.6×10 4/pk
5
VSa950105-T
Promosil HPLC Columns
Using the silica of high-purity and high mechanical strength, the Promosil C18s are made with high-purity
monosilane through Agela Technologies’ well controlled bonding process. They have high surface bonding
coverage and completely capped. The carbon content is as much as 18%. They are stable at pH range
1.5-9.0 showing good peak shape for acidic and basic compounds. They have excellent tolerance of
contamination and long life-time. They are the best choice of high performance-to-cost value.
z
Double End-cap, Good Inertness
z
Good Stability and Reproducibility
z
Excellent Performance-to-cost Value
Technical Parameters:
z
High-purity silica, Metal Impurity<10ppm
z
Average Particle Size: 5μm
z
Specific Surface: 320m2/g
z
Pore Size: 100Å
z
Carbon Loading: 18%
z
pH Range: 1.5-9.0
z
Efficiency: >80, 000/m
z
TF: 0.98-1.20
Hydrophobicity Test
Column:
Promosil C18, 4.6 × 150mm, 5 μm
Sample:
Uracil, Toluene
Mobile phase: MeOH: 25 mM KH2PO4/K2HPO4 ( pH 6.0 )
= 80 : 20
Detection:
UV 254 nm
Flow rate:
1mL/min
Inertness Test
Column:
Promosil C18, 4.6 × 150mm, 5 μm
Sample:
Amitriptyline
Mobile phase: NaH2PO4/Na2HPO4 (20mM, pH 6.9): MeOH
= 32 : 68
Detection:
UV 254 nm
Flow rate:
1mL/min
Surface area: 320m2/g,Pore size: 100Å
Dimension
(mm)
Particle (μm)
Promosil
Promosil
Promosil
Promosil
Promosil
C18
C8
NH2
CN
Silica
2.1×30
5
PM950302-0
PM850302-0
PM750302-0
PM550302-0
PM050302-0
2.1×50
5
PM950502-0
PM850502-0
PM750502-0
PM550502-0
PM050502-0
2.1×100
5
PM951002-0
PM851002-0
PM751002-0
PM551002-0
PM051002-0
2.1×150
5
PM951502-0
PM851502-0
PM751502-0
PM551502-0
PM051502-0
4.6×50
5
PM950505-0
PM850505-0
PM750505-0
PM550505-0
PM050505-0
4.6×100
5
PM951005-0
PM851005-0
PM751005-0
PM551005-0
PM051005-0
4.6×150
5
PM951505-0
PM851505-0
PM751505-0
PM551505-0
PM051505-0
4.6×250
5
PM952505-0
PM852505-0
PM752505-0
PM552505-0
PM052505-0
4.6×10, 4/pk
5
PM950105-0
PM850105-0
PM750105-0
PM550105-0
PM050105-0
Other Manufacturers’ Brands
Agela Technologies may also supply a number of major brand name HPLC columns made by other
manufacturers, as listed below. Please contact us for availability and price information. These brands are:
Zobax, Kromasil, Hypersil, ES-Industry, Shodex, Jordi
Selected Solutions for Popular Applications
Solutions for Highly Water Soluble Compounds:
Venusil AQ C18
Venusil ASB C18
Unisol Amide
It is known that highly water-soluble compounds often have difficulties in HPLC analysis, such as lack of
retention, bad peak shape and inconsistent results. A large scientific effort has been made in developing
HPLC stationary phases that can provide solutions to these problems. However, no single stationary phase
may resolve all these difficulties. Agela Technologies has developed a series of stationary phases along
with development schemes to help chemists systematically develop methods for the analysis of highly
water-soluble compounds. The series includes two reverse stationary phases which are compatible with
100% water and one strong hydrophilic interaction stationary phase which has a neutral functionality on its
surface. Some application examples are included as a guide to your column selection.
Venusil AQ-C18 is a slightly-polar C18 column, and it is our most versatile high aqueous reverse phase
column. Venusil AQ-C18 has a broad pH suitability (1.5-9.0) and thus can be your first option for most of
your HPLC applications. Venusil ASB-C18 has pH range of 0.8-7.0. The column stationary phase is non-end
capped polar C18 and good for low pH conditions. Unisol Amide (HILIC) has a pH range of 2-8 . Unisol
Amide column stationary phase is modified with strong hydrophilic and neutral functionalities, it has
hydrophilic interaction with the compounds. Unisol Amide provides the strongest retention to hydrophilic
compounds among these three types of columns.
In general, your method development may start with a Venusil AQ-C18 column and a mobile phase
containing a mixture of methanol or acetonitrile with an aqueous acidic buffer solution (pH=2-5). This
approach may be applicable to the HPLC analysis of more than 50% of small molecules (<2000 daltons). If
required, you can minimize the percentage of the stronger organic mobile phase to increase retention by
using up to 100% aqueous acidic buffer mobile phase. In the cases that you achieve adequate retention, but
not enough resolution for the compounds of your interest, you may chose to adjust mobile phase ratio,
mobile phase pH (very effective for ionizable compounds), or chose to replace the column with a Venusil
ASB-C18 or Unisol Amide for an alternative selectivity. Figure 1 shows an HPLC separation of four organic
acids using Unisol C18 column in 100% aqueous mobile phase at pH=2.
In the cases that you cannot achieve adequate retention after analysis in 100% aqueous mobile phase using
the Venusil AQ-C18, you have the following options depending on the type of compounds. For acidic
compounds, you may try ASB-C18 columns and lower the pH to further reduce the solubility of the
compounds in water. For such low pH applications, TFA is the preferred acidic modifier. TFA may give you
the highest retention of both acidic and basic compounds than other organic or inorganic acidic modifiers. If
necessary, you may also try a Unisol Amide column in HILIC mode for even higher retention and/or different
selectivity. When using a Unisol Amide column, it will behave like a NP column. More polar compounds will
elute later than less polar compounds. Figure 2 shows a good separation of highly water-soluble shikimic
acid and related substances using a Unisol Amide column. The retention of these compounds was not
adequate for analysis on either an AQ-C18 or an ASB-C18 column.
For basic compounds, you may start with a AQ- C18 column. For compounds with pKa > 9, another
alternative is to use a Unisol Amide column in place of the AQ- C18 column and operate at a low to medium
pH (2-7). Figure 3 shows that even with a 100% aqueous mobile phase, validamycin (basic and highly water
soluable) still has poor retention on a AQ-C18 column. However, a Unisol Amide column operating at pH 2
in the hydrophilic interaction mode resulted in a suitable validamycin separation.
For neutral compounds, changing pH will have a limited effect on retention. If a suitable method cannot be
developed using an AQ-C18 column, an ASB-C18 or Unisol Amide column may be required.
Usually during method development, the desired retention is achieved first, then the mobile phase pH and
ratio is further adjusted to get the desired resolution. When the desired retention or resolution cannot be
obtained for highly water soluble compounds using a AQ-C18 or ASB-C18 columns, a Unisol Amide column
will be a good alternative. Unisol Amide columns offer completely different selectivity from reverse phase
columns and offer much higher retentivity.
The multiple selection of columns and this comprehensive method development scheme for the separation
of highly water-soluble compounds will allow you to develop HPLC methods at ease.
Figure 2. HPLC Analysis of Shikimic Acid
Figure 1. HPLC Separation of Organic Acids
(3,4,5-Trihydroxy-1-cyclohexene-1-carboxylic Acid)
Sample:
Vitamin C, malonic acid, lactic acid, citric
Column:
Column:
Unisol Amide (HILIC) 4.6 x 250 mm, 5
μm
acid.
AQ-C18, 4.6 x 150mm, 5 μm
Mobile phase:ACN/1% formic acid 90-60% in 20 min
Mobile phase:20mmol phosphate buffer，pH=2.0
Detection：
UV 210nm
Detection：
Flow rate:
1.0 mL/min
Flow rate:
UV 210nm
1.0 mL/min
Temperature: 30℃
Temperature: 30℃
Figure 3. HPLC Analysis of Validamycin Raw Products
Column:
Waters Atlantis C18, 4.6 x 250 mm, 5 μm
Column:
Unisol Amide (HILIC) 4.6 x 250 mm, 5 μm
Mobile phase: TFA 1%
Mobile phase: ACN/0.1%TFA 85-40% in 30 min
Flow rate:
Detection：
UV 210nm
Flow rate:
1.0 mL/min
1.0 mL/min
Temperature: Ambient
Other Stationary Phases from Agela Technologies for Hydrophilic
Compounds Separation:
Unisol C18
Venusil XBP-Silica (HILIC II)
Venusil XBP-NH2 (HILIC III)
Venusil ASB C18
DAD1 A, Sig=240, 4 Ref=360,100 (AGELA\07052506.D)
Venusil ASB C18（4.6×250 mm，5 μm）
Column:
(VS952505-0)
Mobile Phase：100mM NH4AC:ACN=95:5
Detection：
240 nm
Flow rate：
1mL/min
Sample:
Melamine in Feed
Unisol C18
Column:
Unisol C18; 4.6 × 150 mm
Mobile Phase: A: NH4AC Buffer (Adjust pH 6.0)
B: ACN
Gradient:
10% B to 60% B in 20 min.
Flow rate:
1.0 mL/min
Sample:
Teicoplanin+Impurity
Temperature: 35℃
Column:
Unisol C18 4.6×150mm ( UO951505-0)
Mobile Phase: Water:ACN:THF (50:50:1,0.1%H3PO4)
Flow Rate:
1mL/min
Sample:
Nicousamide
Detection:
UV 254nm
HILIC Column Family From Agela Technologies
Unisol Amide ( Agela HILIC )
Venusil XBP-Silica ( Agela HILIC II)
Venusil XBP-NH2 (Agela HILIC III)
Comparison:
●Selectivity
XBP-silica is slightly acidic (pH=5.6); while Unisol Amide is slightly basic (pH=7-9), and XBP-NH2 is more
basic (pH=9.2).
XBP-silica has stronger retention of basic compounds, while Unisol Amide and XBP-NH2 have better
retention of acidic compounds.
Unisol Amide has a balanced retention power for neutral, acidic and basic compounds, and thus the highest
versatility.
●Reproducibility and Lifetime
Unisol Amide has the best reproducibility and lifetime because of a bonded and close-to-neutral protection
layer.
Water-soluble Vitamins
●Selectivity comparison on 3 Columns
●Stability comparison of Unisol Amide and silica
Unisol Amide Column,
5μm, 4.6mm×150mm
XBP -NH2 Column,
5μm, 4.6×150mm
XBP-Silica Column,
5μm, 4.6×150mm
Mobile Phase: A: 0.1% TFA in Water ；
B: 0.1% TFA in Acetonitrile ; A:B =90:10
Flow Rate:
1 mL/min
Temperature:
30℃
Detection:
UV 280 nm
Sample:
VB1+VB6+VC+VB2
Stability Comparison of Unisol Amide and Silica
Samples:VB1, VB6, VC, VB2
Aging Conditions:
MeOH:20 mM NaH2PO4 (pH=7.00) = 40 : 60; 1.0mL/min；Temp：40℃；
HPLC Conditions:
Mobile Phase: 0.1%TFA : ACN=90:10
Detection: 280nm; Flow: 1.0 mL/min; Temp: 30?;Inj: 2 μL
Validamycin
Validamycin
Mobile Phase: A: 0.1% TFA in Water
B: Acetonitrile
Gradient:
40% A to 85% A in 30 min
Flow Rate:
1 mL/min
Temperature: 25℃
Detection:
UV 210 nm
Sample:
Validamycin
Solutions for Low pH and High pH Applications
Agela Technologies offers a family of stationary phases for applications to be run at extremely low or
extremely high pH. These columns extend the capability of regular reverse phase HPLC columns with a
typical pH range of 2-8, and provide more options for your applications and method development needs.
Venusil ASB-C18 (pH: 0.8-7.5);
Venusil ASB-C8 (pH: 1.0-7.5);
Durashell RP (pH: 1.5-12.0)
Low pH Stability of ASB-C18
Column:
Venusil ASB-C18, 4.6 ×150 mm, 5 μm
Sample:
Naphthol
Aging:
40℃, TFA in 80% methanol
(pH=1.0), 400 hours
Mobile phase:TFA in 80% methanol (pH=1.5)
Flow rate:
Injection:
1.0 mL/min
5 μL
Temperature: 30℃
High pH Stability of Durashell RP
Column:
Flow rate:
Durashell RP ; 4.6 × 150 mm
1.5 mL /min, 50% ACN/50% 0.05M
Ammonia (pH=9.0)
Sample:
Doxepin, nortriptyline, amitriptyline,
trimipramine ,
Temperature: 35℃
Solutions for LC-MS
Agela Technologies provides columns that can meet the needs of LC-MS applications. The most popular
LC-MS columns from Agela include:
●Unisol C18 (versatile)
●Venusil ASB-C18 and ASB-C8 (extremely low bleed and long lifetime at low pH)
●Unisol Amide (for extremely polar compounds)
Benefits:
●Low bleed and symmetric peak shape→ Sensitivity
●High retention for polar compounds → Sensitivity, versatility, and low ion suppression for bioanalytical
analysis.
●Compatible with 100% water to 100% organic solvents → Simplified method development effort
●Stability → Long column life means a reduced cost for customers
Pseudoephedrine in Plasma
Mass system:
API Qtrap 3200, Applied Biosystem
Mass condition: Positive Ion Mode, MRM
HPLC Column: Venusil ASB C18 Column 2.1mm×50mm
(Part No.: VS950502-0）
Sample:
Sample prepared by SPE (Cleanert
PCX
60mg/3mL; Part No.: CX0603)
Mobile Phase:
A: 1% Formic Acid in Water ；B:
Methanol
Gradient: 20%B to 95%B in 2 min, hold
for
0.5 min, thenswitch to A:B (20:80)
Flow Rate:
0.5 mL/min
Temperature:
25℃
Analysis of 14 Kinds of Hormone
Mass system:
Agilent 1100 Series LC/MSD Trap
HPLC Column: Venusil XBP C18 Column 2.1mm×150mm（ Part No.: VX951502-0）
Sample:
Estriol, Adosterone, Estrone+Estradial, Testosterone, Dehydro-iso-androsterone
Methyl- Testosterone, Epitestosterone,
Medroxyprogestrone+Stanolone,Progesetrone, Androsterone, 5-pregen-3beta-20-one
Mobile Phase: A: Water. ;B: MeOH
Gradient: 40%B to 80%B in 24 min, then hold for 12 min.
Flow Rate:
0.8 mL /min
Temperature: 25℃
Oleic Acid in Plasma
Mass system:
API Qtrap 3200, Applied Biosystem
Mass condition: Negative Ion Mode, MRM
HPLC Column: Venusil ASB C18 Column 2.1mm×50mm（ Part No.: VS950502-0）
Sample:
Sample prepared by SPE (Cleanert PEP 60mg/3mL;Part No.: PE0603)
Mobile Phase: A: 13mmol/L ammonium acetate aq., B: Acetonitrile
Gradient: 5%B to 95%B in 2 min, hold for 2 min., switch to A:B (95:5) then hold for 2 min.
Flow Rate:
0.8 mL/min
Temperature:
25℃
Solutions for Fast Analysis
Agela Technologies provides two approaches for fast HPLC analyses with shorter run times and higher
column efficiencies.
●Small particle size or fused-core technology: Venusil AQ-C18 (2.5μm) , HALO (2.7μm)
●Unique retention mechanism : Optimix C18/Amide
Mobile Phase: A: NH4AC Buffer (Adjust pH 6.0)
B: ACN
Gradient:
10%B to 60%B in 20 min.
Flow rate:
1.0 mL / min
Sample:
Teicoplanin+Impurity
Temperature: 35℃,
Separation by Optimix C18/Amide
Mobile Phase：A: Water, B: Methanol
Sample:
Uracil, Toluene
Flow rate:
1mL/min
Detection:
UV 254nm
t0= 1.52 min
Bring extremely polar and non-polar analytes closer to shorten the analysis time under isocratic conditions
Column: Optimix C18/amide,5μm, 4.6*150mm
Column: C18, 5μm, 4.6*150mm
Mobile Phase: A : B = 50:50
Mobile Phase: A : B= 60 : 40
Solutions for Bio-molecules
Agela Technologies offers a broad line of HPLC columns for Bio-molecules, including reverse phase, normal
phase, ion-exchange, HILIC and size exclusion columns. All column packing materials are made of ultra
pure silica, bonded with pure silanes to ensure the surface inertness.
General guide for the column selection:
1. Small peptide: C18, C8, C4; 100Å or 150 Å
2. Large peptide and proteins: C8, C4, 300 Å; ion-exchange; HILIC; size exclusion
3. Mono- and oligo-saccharides: ion-exchange; NH2; HILIC
4. Polysaccharides: ion-exchange; size exclusion
5. Oligo-nuclei: ion-exchange; reverse phase
6. Nuclei acids: ion-exchange; size exclusion
See the section of “ordering information by the type of stationary phase” for ordering or
call our technical staff for helps in selecting the columns you need.
Solutions for Preparative HPLC (See Purification Section for More Information)
Agela Technologies offers a full line of preparative HPLC columns to meet a variety of application needs.
Our preparative columns feature:
1. Great scalability
2. Excellent bed stability
3. High loading capacity
4. Broad solvent compatibility from Unisol C18, Unisol Amide and Venusil ASB-C18 (100% aqueous to
100% organic solvents)
5. Broad pH range (1-12) from Venusil Durashell
6. Unique selectivity from Unisol Amide and Venusil ASB-C18
Unisol-Amide: an unique phase from Agela Technologies that offers a good alternative selectivity to
reverse phase columns and an excellent solution for purifying highly polar compounds.
Durashell RP: a high pH tolerance enable the use of high pH mobile phase, which results in great
improvement of peak shape and loadability of basic compounds.
Solutions for SFC
Agela Technologies offers a broad line of normal phase columns of different selectivity for SFC applications,
including:
Unisol Amide
Venusil NH2
Venusil Silica
Venusil CN
Venusil Nitrophenyl
They are all made of high purity silica materials and are packed using our robust and reproducible process
to ensure the high efficiency and the extended lifetime.
See the section of “Ordering Information by the Type of Stationary Phase” for ordering or call our
technical staff for helps.
Ordering Information by the Type of Stationary Phases
Reverse Phase Columns
Reverse Phase Columns for Small Molecules
Analytical Columns, Particle Size: 5μm
Dimension
Promosil
Promosil
Promosil
Venusil
Venusil
Venusil
Venusil
(mm)
C18
C8
CN
XBP Phenyl
XBP C4
XBP C1
XBP CN
General C18,
General C8,
100Å, 320
100Å, 320
100Å, 320
100Å, 380
100Å, 380
100Å, 380
100Å, 380
m2/g; C%=10
m2/g; C%=10
m2/g, C%=5
m2/g, C%=12
m2/g, C%=7
m2/g; C%= 3
m2/g, C%=7
2.1×20
PM950202-0
PM850202-0
PM550202-0
VX650202-0
VX450202-0
VX150202-0
VC950202-0
2.1×30
PM950302-0
PM850302-0
PM550302-0
VX650302-0
VX450302-0
VX150302-0
VC950302-0
2.1×50
PM950502-0
PM850502-0
PM550502-0
VX650502-0
VX450502-0
VX150502-0
VC950502-0
2.1×100
PM951002-0
PM851002-0
PM551002-0
VX651002-0
VX451002-0
VX151002-0
VC951002-0
2.1×150
PM951505-0
PM851505-0
PM551505-0
VX651502-0
VX451502-0
VX151502-0
VC951502-0
3.0x30
PM950303-0
PM850303-0
PM550303-0
VX650303-0
VX450303-0
VX150303-0
VC950303-0
3.0x50
PM950503-0
PM850503-0
PM550503-0
VX650503-0
VX450503-0
VX150503-0
VC950503-0
3.0x100
PM951003-0
PM851003-0
PM551003-0
VX651003-0
VX451003-0
VX151003-0
VC951003-0
3.0x150
PM951503-0
PM851503-0
PM551503-0
VX651503-0
VX451503-0
VX151503-0
VC951503-0
4.6×50
PM950505-0
PM850505-0
PM550505-0
VX650505-0
VX450505-0
VX150505-0
VC950505-0
4.6×100
PM951005-0
PM851005-0
PM551005-0
VX651005-0
VX451005-0
VX151005-0
VC951005-0
4.6×150
PM951505-0
PM851505-0
PM551505-0
VX651505-0
VX451505-0
VX151505-0
VC951505-0
4.6×200
PM952005-0
PM852005-0
PM552005-0
VX652005-0
VX452005-0
VX152005-0
VC952005-0
4.6×250
PM952505-0
PM852505-0
PM552505-0
VX652505-0
VX452505-0
VX152505-0
VC952505-0
4.6×10
PM950105-0
PM850105-0
PM550105-0
VX650105-0
VX450105-0
VX150105-0
VC950105-0
ID: 2.1, 3.0, 4.6
CH-100
CH-100
CH-100
CH-100
CH-100
CH-100
CH-100
Column Type
Dimension
Particle
Venusil XBP C18
Venusil XBP
(mm)
Size (μm)
(2)
C18 (L)
ultra surface inertness
and reproducibility;
balanced retention;
100Å, 380 m2/g,
C%=19
same as XBP C18
with larger pore
size; 150Å, 200
m2/g; C%=15
Analytical
2.1×30
5
VX950302-2
VX950302-L
Analytical
2.1×50
5
VX950502-2
VX950502-L
Analytical
2.1×100
5
VX951002-2
VX951002-L
Analytical
2.1×150
5
VX951502-2
VX951502-L
Analytical
3.0x30
5
VX950303-2
VX950303-L
Analytical
3.0x50
5
VX950503-2
VX950503-L
Analytical
3.0x100
5
VX951003-2
VX951003-L
Analytical
3.0x150
5
VX951503-2
VX951503-L
Analytical
4.6×50
5
VX950505-2
VX950505-L
Analytical
4.6×100
5
VX951005-2
VX951005-L
Analytical
4.6×150
5
VX951505-2
VX951505-L
Analytical
4.6×200
5
VX952005-2
VX952005-L
Analytical
4.6×250
5
VX952505-2
VX952505-L
ard Cartridge
4.6×10, 4/pk
5
VX950105-2
VX950105-L
rtridge Holder
20x10
CH-200
CH-200
Dimension
Particle
Venusil
Venusil
Venusil AQ
(mm)
Size (μm)
ASB C18
ASB C8
C18
extreme low
Unisol C18
for high aqueous
universal RP;
pH tolerance
C8 for low pH
applications;
compatible
(0.8-7.5), low
and low bleed;
compatible with
with 100%
2
bleed; 150 Å,
150 Å, 200m /g,
100% water;
water; 100Å,
200m2/g;
C%=7
100Å, 380 m2/g,
410 m2/g,
C%=18
C%=17
C%=12
Durashell
Venusil
Venusil
RP
XBP-C18
XBP-C8
high pH
tolerance
(1.5-12);
150Å, 240
m2/g, C%=15
highest
general RP;
hydrophobicity;
2
100Å, 380
100Å, 380m /g,
m2/g; C%=
C%=22
14
2.1×30
5
VS950302-0
VS850302-0
VA950302-0
UO950302-0
DS950302-0
VX950302-0
VX850302-0
2.1×50
5
VS950502-0
VS850502-0
VA950502-0
UO950502-0
DS950502-0
VX950502-0
VX850502-0
2.1×100
5
VS951002-0
VS851002-0
VA951002-0
UO951002-0
DS951002-0
VX951002-0
VX851002-0
2.1×150
5
VS951502-0
VS851502-0
VA951502-0
UO951502-0
DS951502-0
VX951502-0
VX851502-0
3.0x30
5
VS950303-0
VS850303-0
VA950303-0
UO950303-0
DS950303-0
VX950303-0
VX850303-0
3.0x50
5
VS950503-0
VS850503-0
VA950503-0
UO950503-0
DS950503-0
VX950503-0
VX850503-0
3.0x100
5
VS951003-0
VS851003-0
VA951003-0
UO951003-0
DS951003-0
VX951003-0
VX851003-0
3.0x150
5
VS951503-0
VS851503-0
VA951503-0
UO951503-0
DS951503-0
VX951503-0
VX851503-0
4.6×50
5
VS950505-0
VS850505-0
VA950505-0
UO950505-0
DS950505-0
VX950505-0
VX850505-0
4.6×100
5
VS951005-0
VS851005-0
VA951005-0
UO951005-0
DS951005-0
VX951005-0
VX851005-0
4.6×150
5
VS951505-0
VS851505-0
VA951505-0
UO951505-0
DS951505-0
VX951505-0
VX851505-0
4.6×200
5
VS952005-0
VS852005-0
VA952005-0
UO952005-1
DS952005-0
VX952005-0
VX852005-0
4.6×250
5
VS952505-0
VS852505-0
VA952505-0
UO952505-0
DS952505-0
VX952505-0
VX852505-0
G 4.6×10, 4/pk
5
VS950105-0
VS850105-0
VA950105-0
UO950105-0
DS950105-0
VX950105-0
VX850105-0
2.1×20
3
VS930202-0
VS830202-0
VA930202-0
UO930202-0
DS930202-0
VX930202-0
VX830202-0
2.1×30
3
VS930302-0
VS830302-0
VA930302-0
UO930302-0
DS930302-0
VX930302-0
VX830302-0
2.1×50
3
VS930502-0
VS830502-0
VA930502-0
UO930502-0
DS930502-0
VX930502-0
VX830502-0
2.1×100
3
VS931002-0
VS831002-0
VA931002-0
UO931002-0
DS931002-0
VX931002-0
VX831002-0
2.1×150
3
VS931502-0
VS831502-0
VA931502-0
UO931502-0
DS931502-0
VX931502-0
VX831502-0
3.0x30
3
VS930303-0
VS830303-0
VA930303-0
UO930303-0
DS930303-0
VX930303-0
VX830303-0
3.0x50
3
VS930503-0
VS830503-0
VA930503-0
UO930503-0
DS930503-0
VX930503-0
VX830503-0
3.0x100
3
VS931003-0
VS831003-0
VA931003-0
UO931003-0
DS931003-0
VX931003-0
VX831003-0
3.0x150
3
VS931503-0
VS831503-0
VA931503-0
UO931503-0
DS931503-0
VX931503-0
VX831503-0
G 3.0×10
3
VS930103-0
VS830103-0
VA930103-0
UO930103-0
DS930103-0
VX930103-0
VX830103-0
4.6×50
3
VS930505-0
VS830502-0
VA930505-0
UO930505-0
DS930505-0
VX930505-0
VX830502-0
4.6×100
3
VS931005-0
VS831002-0
VA930505-0
UO931005-0
DS931005-0
VX931005-0
VX831002-0
4.6×150
3
VS931505-0
VS831505-0
VA931505-0
UO931505-0
DS931505-0
VX931505-0
VX831505-0
G4.6×10
3
VS930105-0
VS830105-0
VA930105-0
UO930105-0
DS930105-0
VX930105-0
VX830105-0
CH-100
CH-100
CH-100
CH-100
CH-100
CH-100
GCH 2.1, 3.0,
4.6
10×150
5
VS951510-0
VA951510-0
UO951510-0
DS951510-0
VX951510-0
VX851510-0
10×250
5
VS952510-0
VA952510-0
UO952510-0
DS952510-0
VX952510-0
VX852510-0
20×50
5
VS950520-0
VA950520-0
UO950520-0
DS950520-0
VX950520-0
VX850520-0
20×150
5
VS951520-0
VA951520-0
UO951520-0
DS951520-0
VX951520-0
VX851520-0
20×250
5
VS952520-0
VA952520-0
UO952520-0
DS952520-0
VX952520-0
VX852520-0
30×100
5
VS951030-0
VA951030-0
UO951030-0
DS951030-0
VX951030-0
VX851030-0
30×150
5
VS951530-0
VA951530-0
UO951530-0
DS951530-0
VX951530-0
VX851530-0
30×250
5
VS952530-0
VA952530-0
UO952530-0
DS952530-0
VX952530-0
VX852530-0
G 20×10
5
VS950120-0
VA950120-0
UO950120-0
DS950120-0
VX950120-0
VX850120-0
10×150
10
VS901510-0
UO901510-0
DS901510-0
VX901510-0
VX801510-0
10×250
10
VS902510-0
UO902510-0
DS902510-0
VX902510-0
VX802510-0
20×50
10
VS900520-0
UO900520-0
DS900520-0
VX900520-0
VX800520-0
20×150
10
VS901520-0
UO901520-0
DS901520-0
VX901520-0
VX801520-0
20×250
10
VS902520-0
UO902520-0
DS902520-0
VX902520-0
VX802520-0
30×100
10
VS901030-0
UO901030-0
DS901030-0
VX901030-0
VX801030-0
30×150
10
VS901530-0
UO901530-0
DS901530-0
VX901530-0
VX801530-0
30×250
10
VS902530-0
UO902530-0
DS902530-0
VX902530-0
VX802530-0
50×150
10
VS901550-0
UO901550-0
DS901550-0
VX901550-0
VX801550-0
50×250
10
VS902550-0
UO902550-0
DS902550-0
VX902550-0
VX802550-0
G 20×10
10
VS90120-0
UO900120-0
DS900120-0
VX900120-0
VX800120-0
CH-200
CH-200
CH-200
CH-200
GCH 20x10
CH-200
CH-200
CH-200
G=Guard Cartridge; GCH= Guard Cartridge Holder
Reverse Phase Columns for Large Molecules
2
Particle Size: 5μm; Pore Size: 300Å; Surface Area: 45 m /g
Column
Dimension
Venusil XBP
Venusil
Venusil
Venusil
Type
(mm)
Phenyl
XBP C4
XBP C1
XBP CN
Analytical
2.1×30
VX650302-T
VX450302-T
VX150302-T
VC950302-T
Analytical
2.1×50
VX650502-T
VX450502-T
VX150502-T
VC950502-T
Analytical
2.1×100
VX651002-T
VX451002-T
VX151002-T
VC951002-T
Analytical
2.1×150
VX651502-T
VX451502-T
VX151502-T
VC951502-T
Analytical
3.0x30
VX650303-T
VX450303-T
VX150303-T
VC950303-T
Analytical
3.0x50
VX650503-T
VX450503-T
VX150503-T
VC950503-T
Analytical
3.0x100
VX651003-T
VX451003-T
VX151003-T
VC951003-T
Analytical
3.0x150
VX651503-T
VX451503-T
VX151503-T
VC951503-T
Analytical
4.6×50
VX650505-T
VX450505-T
VX150505-T
VC950505-T
Analytical
4.6×100
VX651005-T
VX451005-T
VX151005-T
VC951005-T
Analytical
4.6×150
VX651505-T
VX451505-T
VX151505-T
VC951505-T
Analytical
4.6×200
VX652005-T
VX452005-T
VX152005-T
VC952005-T
Analytical
4.6×250
VX652505-T
VX452505-T
VX152505-T
VC952505-T
Guard
4.6×10,
Cartridge
4/pk
VX650105-T
VX450105-T
VX150105-T
VC950105-T
Cartridge
2.1, 3.0,
Holder
4.6
CH-100
CH-100
CH-100
CH-100
Semi-prep
10×150
VX651510-T
VX451510-T
VX151510-T
VC951510-T
Semi-prep
10×250
VX652510-T
VX452510-T
VX152510-T
VC952510-T
Semi-prep
20×50
VX650520-T
VX450520-T
VX150520-T
VC950520-T
Semi-prep
20×150
VX651520-T
VX451520-T
VX151520-T
VC951520-T
Semi-prep
20×250
VX652520-T
VX452520-T
VX152520-T
VC952520-T
20×10
VX650120-T
VX450120-T
VX150120-T
VC950120-T
20×10
CH-200
CH-200
CH-200
CH-200
Guard
Cartridge
Cartridge
Holder
2
Particle Size: 5μm; Pore Size: 300Å; Surface Area: 45 m /g
Type
Dimension
Venusil
Venusil
Venusil
Venusil
(mm)
ASB C18
ASB C8
XBP C18
XBP C8
highest
General
hydrophobicity
RP
extreme
low pH
C8 for low
tolerance
pH and
(0.8-7.5),
low bleed
low bleed
Analytical
2.1×30
VS950302-T
VS850302-T
VX950302-T
VX850302-T
Analytical
2.1×50
VS950502-T
VS850502-T
VX950502-T
VX850502-T
Analytical
2.1×100
VS951002-T
VS851002-T
VX951002-T
VX851002-T
Analytical
2.1×150
VS951502-T
VS851502-T
VX951502-T
VX851502-T
Analytical
3.0x30
VS950303-T
VS850303-T
VX950303-T
VX850303-T
Analytical
3.0x50
VS950503-T
VS850503-T
VX950503-T
VX850503-T
Analytical
3.0x100
VS951003-T
VS851003-T
VX951003-T
VX851003-T
Analytical
3.0x150
VS951503-T
VS851503-T
VX951503-T
VX851503-T
Analytical
4.6×50
VS950505-T
VS850505-T
VX950505-T
VX850505-T
Analytical
4.6×100
VS951005-T
VS851005-T
VX951005-T
VX851005-T
Analytical
4.6×150
VS951505-T
VS851505-T
VX951505-T
VX851505-T
Analytical
4.6×200
VS952005-T
VS852005-T
VX952005-T
VX852005-T
Analytical
4.6×250
VS952505-T
VS852505-T
VX952505-T
VX852505-T
4.6×10, 4/pk
VS950105-T
VS850105-T
VX950105-T
VX850105-T
CH-100
CH-100
CH-100
CH-100
Guard
Cartridge
Cartridge
ID: 2.1, 3.0,
Holder
4.6
Semi-Prep
10×150
VS951510-T
VS851510-T
VX951510-T
VX851510-T
Semi-Prep
10×250
VS952510-T
VS852510-T
VX952510-T
VX852510-T
Semi-Prep
20×50
VS950520-T
VS850520-T
VX950520-T
VX850520-T
Semi-Prep
20×150
VS951520-T
VS851520-T
VX951520-T
VX851520-T
Semi-Prep
20×250
VS952520-T
VS852520-T
VX952520-T
VX852520-T
20×10
VS950120-T
VS850120-T
VX950120-T
VX850120-T
20×10
CH-200
CH-200
CH-200
CH-200
Guard
Cartridge
Cartridge
Holder
Normal Phase Columns
Normal Phase Columns for Small Molecules
Particle Size: 5μm
Type
Dimension
(mm)
Particle
Unisol
Venusil
Venusil
Size
Amide
Silica
XBP-NH2(HILIC
(μm)
(HILIC)
(HILIC II)
III)
unique
selectivity;
100Å, 410
m2/g,
ultra
pure ;100Å,
2
380m /g,
ultra pure: 100
Å,380m2/g;
Venusil
Venusil
XBP-Diol
XBP CN
ultra pure:
ultra
100Å, 380
pure:100Å,380
2
m /g
m2/g
Analytical
2.1×30
5
VH950302-0
VSi950302-0
VN850302-0
VC950302-0
VD950302-0
Analytical
2.1×50
5
VH950502-0
VSi950502-0
VN850502-0
VC950502-0
VD950502-0
Analytical
2.1×100
5
VH951002-0
VSi951002-0
VN851002-0
VC951002-0
VD951002-0
Analytical
2.1×150
5
VH951502-0
VSi951502-0
VN851502-0
VC951502-0
VD951502-0
Analytical
3.0x30
5
VH950303-0
VSi950303-0
VN850303-0
VC950303-0
VD950303-0
Analytical
3.0x50
5
VH950503-0
VSi950503-0
VN850503-0
VC950503-0
VD950503-0
Analytical
3.0x100
5
VH951003-0
VSi951003-0
VN851003-0
VC951003-0
VD951003-0
Analytical
3.0x150
5
VH951503-0
VSi951503-0
VN851503-0
VC951503-0
VD951503-0
Analytical
4.6×50
5
VH950505-0
VSi950505-0
VN850505-0
VC950505-0
VD950505-0
Analytical
4.6×100
5
VH951005-0
VSi951005-0
VN851005-0
VC951005-0
VD951005-0
Analytical
4.6×150
5
VH951505-0
VSi951505-0
VN851505-0
VC951505-0
VD951505-0
Analytical
4.6×200
5
VH952005-0
VSi952005-0
VN852005-0
VC952005-0
VD952005-0
Analytical
4.6×250
5
VH952505-0
VSi952505-0
VN852505-0
VC952505-0
VD952505-0
5
VH950105-0
VSi950105-0
VN850105-0
VC950105-0
VD950105-0
CH-100
CH-100
CH-100
CH-100
CH-100
Guard cartridge,
4.6×10 ,
4/pk
GCH
2.1,3.0,4.6
Semi-preparative
10×150
5
VH951510-0
VSi951510-0
VN851510-0
VC951510-0
Semi-preparative
10×250
5
VH952510-0
VSi952510-0
VN852510-0
VC952510-0
Preparative
20×50
5
VH950520-0
VSi950520-0
VN850520-0
VC950520-0
Preparative
20×150
5
VH951520-0
VSi951520-0
VN851520-0
VC951520-0
Preparative
20×250
5
VH952520-0
VSi952520-0
VN852520-0
VC952520-0
Preparative
30×100
5
VH951030-0
VSi951030-0
VN851030-0
VC951030-0
Preparative
30×150
5
VH951530-0
VSi951530-0
VN851530-0
VC951530-0
Preparative
30×250
5
VH952530-0
VSi952530-0
VN852530-0
VC952530-0
Guard Cartridge
20×10
VH950220-0
VSi950220-0
VN850220-0
VC950220-0
Semi-preparative
10×150
10
VH901510-0
VSi901510-0
VN801510-0
VC901510-0
Semi-preparative
10×250
10
VH902510-0
VSi902510-0
VN802510-0
VC902510-0
Preparative
20×50
10
VH900520-0
VSi900520-0
VN800520-0
VC900520-0
Preparative
20×150
10
VH901520-0
VSi901520-0
VN801520-0
VC901520-0
Preparative
20×250
10
VH902520-0
VSi902520-0
VN802520-0
VC902520-0
Preparative
30×100
10
VH901030-0
VSi901030-0
VN801030-0
VC901030-0
Preparative
30×150
10
VH901530-0
VSi901530-0
VN801530-0
VC901530-0
Preparative
30×250
10
VH902530-0
VSi902530-0
VN802530-0
VC902530-0
Preparative
50×150
10
VH901550-0
VSi901550-0
VN801550-0
VC901550-0
Preparative
50×250
10
VH902550-0
VSi902550-0
VN802550-0
VC902550-0
Guard Cartridge
20×10
10
VH900120-0
VSi900120-0
VN800120-0
VC900120-0
GCH
2.1,3.0,4.6
CH-200
CH-200
CH-200
CH-200
GCH=Guard Cartridge Holder
High Purity; Pore Size: 100 Å; Surface Aera: 320m2/g
Type
Dimension
(mm)
Particle
Size
(μm)
Promosil
-CN
Promosil-Silica
Promosil
-NH2
Analytical
2.1×30
5
PM550302-0
PM050302-0
PM750302-0
Analytical
2.1×50
5
PM550502-0
PM050502-0
PM750502-0
Analytical
2.1×100
5
PM551002-0
PM051002-0
PM751002-0
Analytical
2.1×150
5
PM551502-0
PM051502-0
PM751505-0
Analytical
3.0x30
5
PM550303-0
PM050303-0
PM750303-0
Analytical
3.0x50
5
PM550503-0
PM050503-0
PM750503-0
Analytical
3.0x100
5
PM551003-0
PM051003-0
PM751003-0
Analytical
3.0x150
5
PM551503-0
PM051503-0
PM751503-0
Analytical
4.6×50
5
PM550505-0
PM050505-0
PM750505-0
Analytical
4.6×100
5
PM551005-0
PM051005-0
PM751005-0
Analytical
4.6×150
5
PM551505-0
PM051505-0
PM751505-0
Analytical
4.6×200
5
PM552005-0
PM052005-0
PM752005-0
Analytical
4.6×250
5
PM552505-0
PM052505-0
PM752505-0
Guard
4.6×10 ,
cartridge,
4/pk
5
PM550105-0
PM050105-0
PM750105-0
CH-100
CH-100
CH-100
Guard cartridge
Holder
2.1,3.0,4.6
10×150
5
PM051510-0
10×250
5
PM052510-0
Preparative
20×50
5
PM050520-0
Preparative
20×150
5
PM051520-0
Preparative
20×250
5
PM052520-0
Preparative
30×100
5
PM051030-0
Preparative
30×150
5
PM051530-0
Preparative
30×250
5
PM052530-0
20×20
5
PM050220-0
Semi-preparative
Semi-preparative
Guard
Cartridge
Normal Phase Columns for Large Molecules
Ultra Pure Silica; Pore Size:300 Å; Surface Area:45m2/g
Type
Dimension
Particle
Venusil
Venusil
Venusil
Unisol Amide
Venusil
(mm)
Size (μm)
XBP NH2
XBP Diol
XBP CN
(HILIC)
XBP Silica
Analytical
2.1×30
5
VN850302-T
VD950302-T
VC950302-T
VH950302-T
VSi950302-T
Analytical
2.1×50
5
VN850502-T
VD950502-T
VC950502-T
VH950502-T
VSi950502-T
Analytical
2.1×100
5
VN851002-T
VD951002-T
VC951002-T
VH951002-T
VSi951002-T
Analytical
2.1×150
5
VN851502-T
VD951502-T
VC951502-T
VH951502-T
VSi951502-T
Analytical
3.0x30
5
VN850303-T
VD950303-T
VC950303-T
VH950303-T
VSi950303-T
Analytical
3.0x50
5
VN850503-T
VD950503-T
VC950503-T
VH950503-T
VSi950503-T
Analytical
3.0x100
5
VN851003-T
VD951003-T
VC951003-T
VH951003-T
VSi951003-T
Analytical
3.0x150
5
VN851503-T
VD951503-T
VC951503-T
VH951503-T
VSi951503-T
Analytical
4.6×50
5
VN850505-T
VD950505-T
VC950505-T
VH950505-T
VSi950505-T
Analytical
4.6×100
5
VN851005-T
VD951005-T
VC951005-T
VH951005-T
VSi951005-T
Analytical
4.6×150
5
VN851505-T
VD951505-T
VC951505-T
VH951505-T
VSi951505-T
Analytical
4.6×200
5
VN852005-T
VD952005-T
VC952005-T
VH952005-T
VSi952005-T
Analytical
4.6×250
5
VN852505-T
VD952505-T
VC952505-T
VH952505-T
VSi952505-T
Guard Cartridge
4.6×10, 4/pk
5
VN850105-T
VD950105-T
VC950105-T
VH950105-T
VSi950105-T
CH-100
CH-100
CH-100
CH-100
CH-100
ID: 2.1, 3.0,
GCH
4.6
Semi-preparative
10×150
5
VN851510-T
VC951510-T
VSi951510-T
Semi-preparative
10×250
5
VN852510-T
VC952510-T
VSi952510-T
Preparative
20×50
5
VN850520-T
VC950520-T
VSi950520-T
Preparative
20×150
5
VN851520-T
VC951520-T
VSi951520-T
Preparative
20×250
5
VN852520-T
VC952520-T
VSi952520-T
Preparative
30×100
5
VN851030-T
VC951030-T
VSi951030-T
Preparative
30×150
5
VN851530-T
VC951530-T
VSi951530-T
Preparative
30×250
5
VN852530-T
VC952530-T
VSi952530-T
Guard Cartridge
20×10
5
VN850120-T
VC950120-T
VSi950120-T
GCH
20x10
CH-200
CH-200
CH-200
GCH=Guard cartridge Holder
Ion-exchange Columns
Dimension
Particle
Venusil
Venusil
(mm)
Size (μm)
SAX
SCX
Analytical
4.6×100
5
VSa951005-0
VSc951005-0
Analytical
4.6×150
5
VSa951505-0
VSc951505-0
Analytical
4.6×250
5
VSa952505-0
VSc952505-0
4.6×10 4/pk
5
VSa950105-0
VSc950105-0
Dimension
Particle
Venusil
Venusil
(mm)
Size (μm)
SAX
SCX
Analytical
4.6×100
5
VSa951005-T
VSc951005-T
Analytical
4.6×150
5
VSa951505-T
VSc951505-T
Analytical
4.6×250
5
VSa952505-T
VSc952505-T
4.6×10 4/pk
5
VSa950105-T
VSc950105-T
Type
Guard
cartridge
Type
Guard
cartridge
Mixed Phase Columns
Type
Dimension
Particle
Optimix
Optimix
Optimix
Optimix
(mm)
Size(μm)
C18/C8
C18/amide
C18/phenyl
C18/CN
Analytical
2.1×30
5
OP950302-OC
OP950302-AM
OP950302-PH
OP950302-CN
Analytical
2.1×50
5
OP950502-OC
OP950502-AM
OP950502-PH
OP950502-CN
Analytical
2.1×100
5
OP951002-OC
OP951002-AM
OP951002-PH
OP951002-CN
Analytical
2.1×150
5
OP951502-OC
OP951502-AM
OP951502-PH
OP951502-CN
Analytical
4.6×50
5
OP950505-OC
OP950505-AM
OP950505-PH
OP950505-CN
Analytical
4.6×100
5
OP951005-OC
OP951005-AM
OP951005-PH
OP951005-CN
Analytical
4.6×150
5
OP951505-OC
OP951505-AM
OP951505-PH
OP951505-CN
Analytical
4.6×200
5
OP952005-OC
OP952005-AM
OP952005-PH
OP952005-CN
Analytical
4.6×250
5
OP952505-OC
OP952505-AM
OP952505-PH
OP952505-CN
4.6×10
5
OP950105-OC
OP950105-AM
OP950105-PH
OP950105-CN
Fast analysis
2.1×30
3
OP930302-OC
OP930302-AM
OP930302-PH
OP930302-CN
Fast analysis
2.1×50
3
OP930502-OC
OP930502-AM
OP930502-PH
OP930502-CN
Fast analysis
2.1×100
3
OP931002-OC
OP931002-AM
OP931002-PH
OP931002-CN
Fast analysis
2.1×150
3
OP931502-OC
OP931502-AM
OP931502-PH
OP931502-CN
Fast analysis
4.6×50
3
OP930505-OC
OP930505-AM
OP930505-PH
OP930505-CN
Fast analysis
4.6×100
3
OP931005-OC
OP931005-AM
OP931005-PH
OP931005-CN
Fast analysis
4.6×150
3
OP931505-OC
OP931505-AM
OP931505-PH
OP931505-CN
4.6×10
3
OP930105-OC
OP930105-AM
OP930105-PH
OP930105-CN
Guard
cartridge
Guard
cartridge
Type
Dimension
Particle
Optimix
Optimix
Optimix
(mm)
Size(μm)
C18/SCX
C18/SAX
C18/Nitrophenyl
Analytical
2.1×30
5
OP950302-SC
OP950302-SA
OP950302-NP
Analytical
2.1×50
5
OP950502-SC
OP950502-SA
OP950502-NP
Analytical
2.1×100
5
OP951002-SC
OP951002-SA
OP951002-NP
Analytical
2.1×150
5
OP951502-SC
OP951502-SA
OP951502-NP
Analytical
4.6×50
5
OP950505-SC
OP950505-SA
OP950505-NP
Analytical
4.6×100
5
OP951005-SC
OP951005-SA
OP951005-NP
Analytical
4.6×150
5
OP951505-SC
OP951505-SA
OP951505-NP
Analytical
4.6×200
5
OP952005-SC
OP952005-SA
OP952005-NP
Analytical
4.6×250
5
OP952505-SC
OP952505-SA
OP952505-NP
4.6×10
5
OP950105-SC
OP950105-SA
OP950105-NP
Fast analysis
2.1×30
3
OP930302-SC
OP930302-SA
OP930302-NP
Fast analysis
2.1×50
3
OP930502-SC
OP930502-SA
OP930502-NP
Fast analysis
2.1×100
3
OP931002-SC
OP931002-SA
OP931002-NP
Fast analysis
2.1×150
3
OP931502-SC
OP931502-SA
OP931502-NP
Fast analysis
4.6×50
3
OP930505-SC
OP930505-SA
OP930505-NP
Fast analysis
4.6×100
3
OP931005-SC
OP931005-SA
OP931005-NP
Fast analysis
4.6×150
3
OP931505-SC
OP931505-SA
OP931505-NP
4.6×10
3
OP930105-SC
OP930105-SA
OP930105-NP
Guard
cartridge
Guard
cartridge
Size Exclusion Columns (Silica Based)
Type
Dimension
Particle
(mm)
Size (μm)
GF
GF 300
GF amide
GFamide-300
ultra pure,high
ultra pure,high
ultra pure,high
ultra pure,high
porosity;bonded
porosity;bonded
porosity;bonded with
porosity;bonded with
120Å,
300Å,
amide 120Å,
amide 300Å,
Analytical
4.6x 150
5
GF151505-0
GF351505-0
GF151505-A
GF301505-A
Analytical
4.6x 250
5
GF152505-0
GF352505-0
GF152505-A
GF302505-A
Analytical
4.6x 150
10
GF101505-0
GF301505-0
GF101505-A
GF301505-A
Analytical
4.6x 250
10
GF102505-0
GF302505-0
GF102505-A
GF302505-A
Semi-prep.
10x150
5
GF151510-0
GF351510-0
GF151510-A
GF301510-A
Semi-prep.
10x250
5
GF152510-0
GF352510-0
GF152510-A
GF302510-A
Semi-prep.
10x150
10
GF101510-0
GF301510-0
GF101510-A
GF301510-A
Semi-prep.
10x250
10
GF102510-0
GF302510-0
GF102510-A
GF302510-A
Polymer Phase Columns
RPC-Analytical Prepacked Columns
Dimension
Particle
(mm)
Size (μm)
AKF-RP-M-154607
4.6×150
7
AKF-RP-M-154610
4.6×150
10
AKF-RP-M-154620
4.6×150
20
AKF-RP-H-254605
4.6×250
5
AKF-RP-H-254607
4.6×250
7
AKF-RP-H-254610
4.6×250
10
AKF-RP-H-254620
4.6×250
20
Matrix
RPC-Preparative Prepacked Columns
Matrix
Dimension
(mm)
Particle Size (μm)
AKF-RP-M-052007
20×50
5
AKF-RP-M-102007
20×100
5
AKF-RP-M-251007
10×250
7
AKF-RP-M-252007
20×250
7
AKF-RP-M-151010
10×150
10
AKF-RP-M-152010
20×150
10
AKF-RP-H-151010
10×150
10
AKF-RP-H-152010
20×150
10
AKF-RP-M-251010
10×250
10
AKF-RP-M-252010
20×250
10
AKF-RP-H-251010
10×250
10
AKF-RP-H-252010
20×250
10
AKF-RP-H-152020
20×150
20
AKF-RP-H-252020
20×250
20
AKF-RP-H-105020
50×100
20
AKF-RP-H-255020
50×250
20
AKF-RP-H-2510020
100×250
20
Hydrophobic Interaction Chromatography Columns
Dimension
Particle Size
(mm)
(μm)
AKF-PHEMM-154607
4.6×150
7
AKF-PHEMM-154610
4.6×150
10
AKF-PHEMM-154620
4.6×150
20
AKF-PHEMH-254607
4.6×250
7
AKF-PHEMH-254610
4.6×250
10
AKF-PHEMH-254620
4.6×250
20
AKF-PHEMH-036410
6.4×30
10
AKF-C4-MM-154607
4.6×150
7
AKF-C4-MM-154610
4.6×150
10
AKF-C4-MM-154620
4.6×150
20
AKF-C4-MH-254607
4.6×250
7
AKF-C4-MH-254610
4.6×250
10
AKF-C4-MH-254620
4.6×250
20
AKF-C4-MH-036410
6.4×30
10
AKF-C8-MM-154607
4.6×150
7
AKF-C8-MM-154610
4.6×150
10
AKF-C8-MM-154620
4.6×150
20
AKF-C8-MH-254607
4.6×250
7
AKF-C8-MH-254610
4.6×250
10
AKF-C8-MH-254620
4.6×250
20
AKF-C8-MH-036410
6.4×30
10
AKF-ETHMM-154607
4.6×150
7
AKF-ETHMM-154610
4.6×150
10
AKF-ETHMM-154620
4.6×150
20
AKF-ETHMH-254607
4.6×250
7
AKF-ETHMH-254610
4.6×250
10
AKF-ETHMH-254620
4.6×250
20
AKF-ETHMH-036410
6.4×30
10
Matrix
Ion Exchange Chromatography Columns (strong cation)
Matrix
Dimension
Particle Size
(mm)
(μm)
AKF-CISLM-050207
2.0×50
5
AKF-CISLM-150207
2.0×150
5
AKF-CISLM-154607
4.6×150
7
AKF-CISLM-154610
4.6×150
10
AKF-CISLM-154620
4.6×150
20
AKF-CISLH-154607
4.6×150
7
AKF-CISLH-154610
4.6×150
10
AKF-CISLH-154620
4.6×150
20
AKF-CISLM-254610
4.6×250
10
AKF-CISLH-254610
4.6×250
10
AKF-CISLH-251010
10×250
10
AKF-CISLH-101010
10×100
10
Ion Exchange Chromatography Columns (Weak cation)
Dimension
Particle Size
(mm)
(μm)
AKF-CLV-050207
2.0×50
5
AKF-CLV-150207
2.0×150
5
AKF-CLV-154607
4.6×150
7
AKF-CLV-154610
4.6×150
10
AKF-CLV-154620
4.6×150
20
AKF-CHV-154607
4.6×150
7
AKF-CHV-154610
4.6×150
10
AKF-CHV-154620
4.6×150
20
AKF-CLV-254610
4.6×250
10
AKF-CHV-254610
4.6×250
10
AKF-CHV-251010
10×250
10
AKF-CHV-101010
10×100
10
Matrix
Ion Exchange Chromatography Columns (Strong Anion)
Dimension
Particle
(mm)
Size (μm)
AKF-QLV-050207
2.0×50
5
AKF-QLV-150207
2.0×150
5
AKF-QLV-154607
4.6×150
7
AKF-QLV-154610
4.6×150
10
AKF-QLV-154620
4.6×150
20
AKF-QHV-154607
4.6×150
7
AKF-QHV-154610
4.6×150
10
AKF-QHV-154620
4.6×150
20
AKF-QLV-254610
4.6×250
10
AKF-QHV-254610
4.6×250
10
AKF-QHV-251010
10×250
10
AKF-QHV-101010
10×100
10
Matrix
Ion Exchange Chromatography Columns (Weak Anion)
Matrix
Dimension
Particle
(mm)
Size (μm)
AKF-DEAL-050207
2.0×50
5
AKF-DEAL-150207
2.0×150
5
AKF-DEAL-154607
4.6×150
7
AKF-DEAL-154610
4.6×150
10
AKF-DEAL-154620
4.6×150
20
AKF-DEAH-154607
4.6×150
7
AKF-DEAH-154610
4.6×150
10
AKF-DEAH-154620
4.6×150
20
AKF-DEAL-254610
4.6×250
10
AKF-DEAH-254610
4.6×250
10
AKF-DEAH-251010
10×250
10
AKF-DEAH-101010
10×100
10
HPLC Column Selection Guide
Column Selection Parameters
Stationary Phases
z
Reverse Phase [C18 (XBP, AQ, ASB), C8 (XBP, ASB), C4 , Phenyl ]: most HPLC analytical and
preparative separations; use shorter chain if the retention is too high on C18 columns；use shorter
chain (C8, C4) for proteins and larger peptides.
z
Normal Phase [Silica, Amino, SAX, SCX]: for those not applicable on reverse phase; polysaccharides
(amino), ion-exchange chromatography, some preparative needs.
z
Bi-mode [Cyano, Venusil HILIC]: can be used in both reverse and normal phase modes, alternative
selectivity to hydrocarbon-based reverse phases, inert and better reproducibility than silica columns.
Particle Size
z
3 μm: fast analysis, high throughput analytical applications, micro/nano HPLC
z
5 μm: analytical and semi-preparative separation
z
10 μm: preparative separation
Pore Size
z
Narrow pore (100-150 Å): MW <3000
z
Large pore (300-500 Å): 3000< MW <50000
z
Mega pore (1000 Å): MW >50000
Column Length
z
Short (30 mm, 50 mm): high throughput analysis and purification, pre-separation
z
Regular (100 mm, 150 mm, 250 mm): more complex sample, larger injection volume
Column Diameter
z
Capillary (0.5 mm, 1.0 mm): LC-MS, micro-HPLC, very small sample volume
z
Analytical (2.1 mm, 4.6 mm, 10 mm): standard HPLC, analytical and mini-prep
z
Preparative (21 mm, 30 mm, 50 mm): preparative HPLC
Basic Considerations in Choosing HPLC Columns
Analyte
z
Molecular Weight – small molecule (<3000), narrow pore; medium molecule(3000- 50000), large
pore; large molecule(>50000), mega pore.
z
Solubility in aqueous solutions – very hydrophilic, use AQ-C18, ASB-C18 or Venusil HILIC; very
hydrophobic, use shorter chain phases (C8, C4, Phenyl, CN); in between, use AQ-C18, ASP-C18,
XPB-C18 or Phenyl .
z
Difference between the compounds to be separated – by polarity, use AQ C18, ASB-C18, cyano,
HILIC; by shape or regio-isomer, use XBP-C18, phenyl.
Mobile Phase
•
Solution solvent: 97-100% aqueous solution, AQ-C18 or ASB-C18; normal phase mode with
aqueous mobile
z
phase, Venusil HILIC
•
pH: pH < 2, C18 phases; pH > 9, XBP-C18; pH= 2-9, most phases; (check the pH range for each
column
z
before use!)
•
Salt concentration: high salt concentration >0.1 M (should be avoided if possible), XBP-C18,
SCX, SAX.
Sample
z
Analyte mass/concentration: high mass load, larger diameter column (>10 for preparative).
z
Sample volume: small volume, small diameter; large volume, large diameter and length.
z
Sample complexity: simple separation, short column; complex separation, long column.
Instrument and Application
z
Traditional HPLC analysis: 3μm, 5μm; 4.6 x 100 mm, 4.6 x 150 mm, 4.6 x 250 mm
z
High throughput analysis: 3μm, 5μm; 2.1 x 30 mm, 2.1 x 50 mm, 4.6 x 50 mm.
z
LC-MS application: 3μm, 5μm; 1.0 x 30 mm, 1.0 x 50 mm, 2.1 x 30 mm, 2.1 x 50 mm, 2.1 x 100 mm.
z
Micro HPLC: 3μm, 5μm; 0.5 x 30 mm, 0.5 x 50 mm, 0.5 x 100 mm.
z
Preparative HPLC: 5μm, 10μm; 21 x 50 mm, 21 x 150 mm, 21 x 250 mm, 30 x 150 mm, 30 x 250 mm,
50 x 250 mm.
Cross-reference for Agela Venusil Series Columns
XBP C18
XBP C18 (2)
XBP C8
XBP(L) C18
XBP(L) C8
ASB C18
ASB C8
AQ C18
HILIC
Agilent
SB-C18
R
SB-C8
R
XDB-C18
R
XDB-C8
R
SB-Aq
R
Waters
Sunfire-C18
R
R
Sunfire C8
R
Atlantis-C18 (AQ)
E
Atlantis-HILIC
R
Phenomenex
Luna-C18
E
Luna-C8
E
Luna-C18 (2)
E
Supelco
Discovery-C18
E
Discovery-C8
Asentics-C18
Asentics-C8
E
E
E
GL Science
Insertsil ODS-3
R
Comparison of Particle Characteristics of Typical HPLC Stationary Phases
Stationary Phase
Particle
(μm)
Pore(A)
Surface
2
C%
(m /g)
End
High Purity
Capping
Silica
Venusil XBP-C18 (2)
5
100
380
19
double
yes
Venusil XBP-C18
5
100
380
22
double
yes
Venusil AQ-C18
5
100
380
18
yes
yes
Venusil ASB-C18
5
150
200
11
no
yse
Venusil XBP-C18(L)
5
150
200
14
double
yes
ZORBAX SB-C18
5
80
180
10
no
yes
ZORBAX XDB-C18
5
80
180
12
yes
yes
Waters SunFire C18
5
100
340
16
yes
yes
Waters Symmetry C18
5
100
335
19
yes
yes
Waters XTerra MS C18
5
125
—
15.5
yes
—
Hypersil BDS C18
5
130
170
11
yes
no
Hypersil ODS
5
120
170
10
yes
no
Inertsil ODS
5
100
350
14
yes
no
Inertsil ODS3
5
100
450
15
yes
yes
Kromasil C18
5
100
340
19
yes
special
Supelco Discovery C18
5
180
200
12
yes
yes
Luna C18(2)
5
100
400
17.5
yes
yes
USP Column Selection Guide
USP
L01
Specifications
Octadecyl silane bonded to porous silica or ceramic
microparticles, 3 to 10μm in diameter.
Column
Venusil C18
Particle
Shape
Spherical
Octadecyl silane chemically bonded to silica gel of a controlled
L02
surface porosity that has been bonded to a solid spherical core, 30
Please call
to 50μm in diameter
L03
L04
L05
Porous silica microparticles, 5 to 10 μm in diameter
Silica gel of controlled surface porosity bonded to a solid
spherical core, 30 to 50μm in diameter.
Alumina of controlled surface porosity bonded to a solid
spherical core, 30 to 50 μm in diameter.
Venusil silica
Spherical
Please call
Please call
Strong cation-exchange packing: sulfonated fluorocarbon
L06
polymer coated on a solid spherical core, 30 to 50 μm in
Please call
diameter.
L07
Octylsilane bonded to totally porous microsilica particles, 3 to 10
μm in diameter.
Venusil C8
Spherical
Venusil NH2
Spherical
Please call
Irregular
Venusil CN
Spherical
Venusil Phenyl
Spherical
An essentially monomolecular layer of aminopropyl-silane
L08
chemically bonded to totally porous silica gel support, 10 μm in
diameter.
L09
L10
L11
10 μm irregular totally porous silica gel having a chemically
bonded, strongly acidic cation-exchange coating.
Nitrile groups chemically bonded to porous silica microparticles,
3 to 10 μm in diameter.
Phenyl groups chemically bonded to porous silica
micro-particles, 3 to 10 μm in diameter.
Strong anion-exchange packing made by chemically bonding a
L12
quaternary amine to a solid silica spherical core, 30 to 50 μm in
Please call
diameter
L13
L14
L15
L16
Trimethylsilane chemically bonded to porous silica
microparticles, 3 to 10 μm in diameter.
Silica gel, 10 μm in diameter, having a chemically bonded,
strongly basic quaternary ammonium anion-exchange coating.
Hexyl silane chemically bonded to totally porous silica particles,
3 to 10 μm in diameter.
Dimethyl silane chemically bonded to totally porous silica
particles, 3 to 10 μm in diameter.
Please call
Spherical
Please call
Spherical
Please call
Please call
Spherical
Strong cation exchange resin consisting of sulfonated
L17
cross-linked styrene-divinylbenzene copolymer in the hydrogen
form, 7 to 11 μm in diameter.
L18
Amino and cyano groups chemically bonded to porous silica
L19
cross-linked styrene-divinylbenzene copolymer in the calcium
form, 9 μm in diameter.
L20
Dihydroxypropane groups chemically bonded to
AKF CISLH
columns
Please call
AKF CISLM
columns
Please call
Spherical
Irregular
Spherical
Spherical
USP Column Selection Guide
USP
L21
L22
Specifications
A rigid, spherical styrene-divinylbenzene copolymer, 5
to 10 μm in diameter.
A cation exchange resin made of porous polystyrene gel
with sulfonic acid groups, about 10 μm in size.
Column
AKF RP
columns
AKF CISLH
columns
Particle
Shape
Spherical
Spherical
An ion exchange resin made of porous polymethacrylate
L23
or polyacrylate gel with quaternary ammonium groups,
Please call
Spherical
about 10 μm in size.
A semi-rigid hydrophilic gel consisting of vinyl
L24
polymers with numerous hydroxyl groups on the matrix
Please call
surface, 32 to 63 μm in diameter.
Packing having the capacity to separate compounds with
L25
a MW range from 100 to 5000 daltons (as determined by
Please call
Spherical
Venusil C4
Spherical
polyethylene oxide), applied to neutral
L26
L27
Butyl silane chemically bonded to totally porous silica
Porous silica particles, 30 to 50 μm in diameter.
Please call
A multifunctional support, which consists of a high
L28
purity, 100 Å spherical silica substrate that has been
Please call
bonded with anionic (amine) functionality.
Gamma alumina, reversed phase, low carbon percentage
L29
by weight, alumina-based polybutadiene spherical
Please call
particles, 5 μm diameter w/a pore diameter of 80 Å
L30
Ethyl silane chemically bonded to a totally porous silica
particle, 3 to 10 μm in diameter.
Please call
Irregular
A strong anion-exchange resin-quaternary amine bonded
L31
on latex particles attached to a core of 8.5 μm
Please call
macroporous particles having a pore size of 2000 Å.
A chiral ligand-exchange packing- L-proline copper
L32
complex covanlently bonded to irregularly shaped silica
Please call
Spherical
Please call
Spherical
Please call
Spherical
Please call
Spherical
Packing having the capacity to separate proteins of 4,000
L33
to 400,000 daltons. It is spherical, silicabased and
processed to provide pH stability.
L34
cross-linked styrene-divinylbenzene copolymer in the lead
form, 9 μm in diameter.
A zirconium-stabilized spherical silica packing with a
L35
hydrophilic (diol-type) molecular monolayer bonded
phase having a pore size of 150 Å.
L36
L-Phenylglycine-3,5-dinitrobenzoyl on 5 μm
aminopropyl silica
Nucleosil
Chiral-3
Spherical
L37
Polymethacrylate gel for proteins 2000~40,000 MW
Please call
Spherical
L38
Methacrylate-based SEC column for water-solubles
Please call
Spherical
Please call
Spherical
L39
L40
Hydrophilic polyhydroxymethacrylate gel of totally
porous spherical resin
Cellulose tris-3,5 dimethylphenylcarbamate coated
Please call
Applications
1.Antibiotics
1.1 Benzylpenicillin Sodium
Column:
Unisol C18（4.6×150mm，5μm）
Part No.:
UO951505-0
Mobile phase: 0.1mol/L potassium dihydrogen phosphate
buffer
（pH 2.5）:ACN＝70:30
Detection:
UV 225 nm
Flow rate:
1 mL/min
Injection:
4 μL
1.2 Amoxicillin Sodium
Column:
Part No.:
UO951505-0
Mobile phase: Phosphate buffer （0.05mol/L ,pH5.0）:
ACN=97.5:2.5
Detection:
UV 254 nm
Flow rate:
1 mL/min
Injection:
5 μL
Column:
Part No.:
UO951505-0
1.3 Cefradine
Mobile phase: Water/MeOH/3.86% sodiumacetate
solution/4％
acetic acid solution＝742:240:15:3
Detection:
UV 254 nm
Flow rate:
1 mL/min
Injection:
2μL
Column:
Part No.:
UO951505-0
1.4 Cefaclor
Mobile phase: 0.05mol/L phosphate buffer（pH 3.4）:
ACN＝92:8
Detection:
UV 254 nm
Flow rate:
1 mL/min
Injection:
5μL
1.5 Cefadroxil
Column:
Part No.:
UO951505-0
Mobile phase: 0.05mol/L phosphate buffe（r pH 5.5）:
CAN=96:4
Detection:
UV 230 nm
Flow rate:
1 mL/min
Injection:
2μL
Column:
Part No.:
UO951505-0
1.6 Cefalexin
Mobile phase: Water/MeOH/3.86% sodium acetate
solution/4％
acetic acid solution=742:240:15:3
1.7 Cefotaxime Sodium
Detection:
UV 254 nm
Flow rate:
1 mL/min
Injection:
2 μL
Column:
Part No.:
UO951505-0
Mobile phase: phosphate buffer（0.4mM KH2PO4+8mM
K2HPO4）
/MeOH=89:11
1.8 Sulbactam Sodium + Cefoperazone Sodium
Detection:
UV 254 nm
Flow rate:
1 mL/min
Injection:
2 μL
Column:
Venusil XBP C18, (4.6×250 mm，5 μm)
Part No.:
VX952505-0
Mobile phase: 0.005 mol/L TABOH (adjust pH to 5.0 with
H3PO4):
ACN =70:30
Detection:
UV 220nm
Flow rate:
1 mL/min
Injection:
2 μL
1.9 Acetylspiramycin
Column:
Part No.:
UO952505-0
Mobile phase: ACN:NaClO4/ H3PO4 （pH 2.2）
=30:70
Temperature: 30℃
Flow rate:
0.8 mL/min
Injection:
20 μL
Column:
Venusil XBP-C8, (4.6×150 mm，5 μm)
Part No.:
VX851505-0
1.10 Meleumycin
Mobile phase: ammonium formate（0.2mol/L，
adjust to pH 7.3 with TEA）:ACN=62:38
Detection:
UV 232nm
Flow rate:
1 mL/min
Temperature: 30℃
2.Synthetic antimicrobial agents
2.1 Norfloxacin
Column:
Part No.:
VX952505-0
Mobile phase: H3PO4 (0.025 mol/L，adjust to pH 3.0 with
TEA):ACN＝87:13
Detection:
UV 278nm
Flow rate:
1 mL/min
Injection:
20 μL
2.2 Ofloxacin
Column:
Part No.:
VX952505-0
Mobile phase ：NH4AC/ KClO4 (40mM , pH 2.0):ACN=85:15
Detection:
UV 294nm
Flow rate:
1 mL/min
Injection:
4 μL
2.3 Ciprofloxacin Lactate
Column:
Part No.:
VX951505-0
Mobile phase: citric acid buffer (0.05 mol/L, adjust to pH 3.5
with TFA):ACN=82:28
2.4 Gatifloxacin hydrochloride
Detection:
UV 277nm
Flow rate:
1 mL/min
Injection:
10 μL
Column:
Venusil XBP C18(L),(4.6×150mm，5 μm)
Part No.:
VX951505-L
Mobile phase:1%TEA（pH 4.5）:ACN=87:13
Detection:
UV 325nm
Flow rate:
1.5 mL/min
Injection:
10 μL
Temperature: 40℃
2.5 Fluconazole
Column:
Part No.:
VX951505-0
Mobile phase: KH2PO4 buffer(adjust to pH7.0 with
NaOH):MeOH＝55:45
Detection:
UV 260nm
Flow rate:
1 mL/min
Injection:
10 μL
Column:
Venusil XBP-C8, (4.6×250 mm，5 μm)
Part No.:
VX852505-0
3.Anti-virus medicine
3.1 Rifampicin
Mobile phase: MeOH/ACN/KH2PO4 （0.075 mol/L）/citric acid
（1mol/L）＝30:30:36:4
Detection:
UV 254nm
Flow rate:
1 mL/min
Injection:
2 μL
3.2 Ganciclovir
Column:
Part No.:
UO951505-0
Mobile phase:Water:MeOH＝95:5
Detection:
UV 252nm
Flow rate:
1 mL/min
Injection:
2 μL
Column:
Part No.:
UO951505-0
4. Steriod Hormones
4.1 Ethinylestradiol
Mobile phase:MeOH:Water＝70:30
4.2 Triamcinolone Acetonide
Detection:
UV 281nm
Flow rate:
1 mL/min
Injection:
2 μL
Column:
Part No.:
UO951505-0
Mobile phase: MeOH:Water＝21:19
Detection:
UV 240nm
Flow rate:
1 mL/min
Injection:
2 μL
Column:
Part No.:
UO951505-0
4.3 Hydrocortisone
Mobile phase: MeOH:Water＝70:30
Detection:
UV 240nm
Flow rate:
1 mL/min
Injection:
10 μL
4.4 Dexamethasone
Column:
Part No.:
VX951505-0
Mobile phase: citric acid buffer (0.05 mol/L, adjust to pH
3.5 with TFA):ACN=82:28
Detection:
UV 277nm
Flow rate:
1 mL/min
Injection:
10 μL
4.5 Levonorgestrel
Column:
Part No.:
UO951505-0
Mobile phase:MeOH:Water＝70:30
Detection:
UV 281nm
Flow rate:
1 mL/min
Injection:
2 μL
Column:
Part No.:
UO951505-0
4.6 Prednisone
Mobile phase: Water :THF :MeOH＝668:250:62
Detection:
UV 254nm
Flow rate:
1 mL/min
Injection:
2 μL
Column:
Part No.:
UO951505-0
4.7 Clobetasol Propionate
Mobile phase: phosphate buffer（0.05 mol/L ,pH 2.5）
/ACN/MeOH＝425:475:100
Detection:
UV 240nm
Flow rate:
1mL/min
Injection:
2μL
4.8 Fluocinonide
Column:
Venusil XBP C18 （4.6×150mm，5μm）
Part No.:
VX951505-0
Mobile phase: MeOH :ACN :Water=60:10:30
Detection:
UV 240nm
Flow rate:
1 mL/min
Injection:
10 μL
5 Medicine for Gastric Ulcer
5.1 Ranitidine Hydrochloride
Column:
Venusil XBP C18（4.6×150mm，5μm）
Part No.:
VX951505-0
Mobile phase: MeOH :0.77%
Ammonium acetate aq.＝285:115
Detection:
UV 320nm
Flow rate:
1 mL/min
Injection:
10 μL
5.2 Cimetidine
Column:
Venusil XBP C18 （4.6×150mm，5μm）
Part No.:
VX951505-0
Mobile phase:
MeOH:Water:H3PO4:TFA
＝200:800:0.3:0.2
Detection:
UV 220nm
Flow rate:
1mL/min
Injection:
10 μL
5.3 Omeprazole
Column:
Venusil XBP-C8（4.6×250mm，5μm）
Part No.:
VX852505-0
Mobile phase: NaH2PO4 Buffer (0.01 mol/L, adjust to
pH7.6 with H3PO4): ACN＝60:40
Detection:
UV 302nm
Flow rate:
1 mL/min
Injection:
4 μL
6. Analysis of Alkaloids
6.1 Quaternary Ammonium Alkaloids from Coptidis
Column:
Part No.:
UO952505-0
Mobile phase: water(0.3%TEA):ACN=75:25
Detection:
UV 254nm
Flow rate:
1 mL/min
Temperature: 25℃
6.2 Matrine
Column:
Venusil XBP-NH2（4.6×250mm，5μm）
Part No.:
VN952505-0
Mobile phase:ACN:Ethanol:3% H3PO4 aq.＝80:10:10
Detection:
UV 220nm
Flow rate:
1 mL/min
Injection:
20 μL
6.3 Ephedrine from Coptidis
Mobile phase:
ACN: SDS/H3PO4＝40:60
Detection:
UV 210nm
Flow rate:
1mL/min
6.4 Berberine from Phellodendron
Mobile phase:
ACN: 1%TFA＝35:65
Detection:
UV 346nm
Flow rate:
1mL/min
6.5 Aconitine、Mesaconitine、Hypaconitin
Mobile phase:
ACN: 2% Acetate Buffer （adjust pH to
6.5 with TEA）＝15:85
Detection:
UV 235nm
Flow rate:
1 mL/min
6.6 Morphine from Papaver somniferum
Mobile phase: MeOH: CH3COONa aq.(0.03mol/L, adjust
pH to 3.5with Acetic acid) =15:85
Detection:
UV 238nm
Flow rate:
1 mL/min
7.Agricultural Chemical
Sample:
dazomet、Cymoxanil、Triadimefon、Tebuconazole、
Thiophanate-Methyl、hexaconazole、flutriafol、Iprodione、
7.1 Analysis of 14 Kinds Herbicides
Metalaxyl、Procymidone、carboxin、Prochlora、
Diethofencar、Triadimenol、
1,2,3,6-tetrahydro-N-(trichloromethylthio)phthalimide
Column:
Venusil XBP C18（4.6×150mm，5μm）
Part No.:
VX951505-0
Mobile phase: A: Ammonium acetate buffer B:ACN
Gradient: 40%B to 45%B in 8 min, hold for 40min.
7.2 Haloxyfop-P-methyl
Detection:
UV 225nm
Injection:
20 μL
Flow rate: 1 mL/min
Temperature:
25℃
Column:
Venusil XBP C18（4.6×150 mm，5 μm）
Part No.:
VX951505-0
Mobile phase: ACN:Water=70:30
Detection:
UV 225nm
Flow rate:
1 mL/min
Injection:
5 μL
Temperature:
25℃
7.3 Fluazifop-p-butyl
Column: Venusil XBP C18（4.6×150 mm，5 μm）
Part No.: VX951505-0
Mobile phase:ACN:Water=70:30
Detection: UV 225nm
Flow rate: 1 mL/min
Injection: 5 μL
Temperature: 25℃
7.4 1,2-Dibenzoyl-1-tert-butylhydrazine
Column:
Venusil XBP C18（4.6×150 mm，5 μm）
Part No.:
VX951505-0
Mobile phase:Water:MeOH =25:75
Detection:
UV 254nm
Flow rate:
1 mL/min
Injection:
10 μL
Temperature: 25℃
8.Analysis of Amino Acid
Column:
Venusil AA（4.6×250 mm，5 μm）
Part No.:
AA952505-0
Mobile phase: A: 0.1mol/L CH3COONa(Adjust pH 6.5 with Acetic Acid):Acetonitrile (93:7)
B:Water: Acetonitrile (4:1) Gradient:
Time(min)
0
11
13.9
14
29
29.1
37
37.1
45
%B
0
7
12
15
34
100
100
0
0
Flow rate:
1mL/min
Detection:
UV 254nm
Temperature: 40℃
Sample:
PTC-AA (derivatization of amino acids with PITC)
8.1 Standards of Amino Acids
8.2 Amino Acid from Salmon Calcitonin
8.3 Amino Acid from Terlipressin
9 Applications in LC-MS
9.1 Pseudoephedrine in Plasma
Mass system:
API Qtrap 3200 Applied Biosystem
Mass condition: Cation Detector Mode,MRM
HPLC Column: Venusil ASB C18 Column 2.1mm×50mm （Part No.: VS950502-0）
Sample:
Prepared sample by SPE(Cleanert PCX 60mg/3ml;Part No.: CX0603)
Mobile Phase: A: 1% Formic Acid in Water ；B: Methanol
Gradient: 20%B to 95%B in 2 min, hold for 0.5 min, then switch to A:B(20:80)
Flow Rate:
0.5 mL/min
Temperature: 25℃
9.2 Oleic Acid in Plasma
Mass system:
API Qtrap 3200 Applied Biosystem
Mass condition:
Anion Detector Mode,MRM
HPLC Column: Venusil ASB C18 Column 2.1mm×50mm （Part No.: VS950502-0）
Sample:
Prepared sample by SPE(Cleanert PEP 60mg/3ml;Part No.: PE0603)
Mobile Phase: A: 13mmol/L ammonium acetate aq. ;B: Acetonitrile
Gradient: 5%B to 95%B in 2 min, hold for 2 min., switch to A:B(95:5) then hold for 2 min.
Flow Rate:
0.8 mL/min
Temperature:
25℃
10.others
10.1 Glipizide
Column:
Part No.:
UO951505-0
Mobile phase: phosphate Buffer（0.1 mol/L ,pH
6.0）:MeOH=55:45
Flow rate:
1mL/min
Injection:
20 μL
Column:
Part No.:
UO951505-0
10.2 Terfenadine
Mobile phase:MeOH: H3PO4/Triethylamine
(0.1mol/L)＝80:20
Detection:
UV 254nm
Flow rate:
1 mL/min
Injection:
10 μL
10.3 Dopamine and Its Metabolin
Sample:
Dopamine、Isoprenaline、Soprenaline
Column:
Venusil F5PH（4.6×250mm，5μm）
Part No.:
VF952505-0
Mobile phase: MeOH: CH3COOH /CH3COONH4 Buffer
(pH 4.5) =15:85
Detection:
UV 280nm
Flow rate:
0.8 mL/min
10.4 Organic Acids
Determination of organic acids in Fermentation Broth
Sample:
Formic acid, acetic acid, maleic acid,
succinic acid, fumaric acid
Column:
Unisol C18（4.6×250 mm，5 μm）
Part No.:
UO951505-0
Mobile phase: 0.02M NH4AC Aq.:MeOH=95:5
Detection:
UV 215nm
Flow rate:
1 mL/min
Injection:
10 μL
Temperature: 20℃
10.5 Catechins in Tea
Sample:
Extract of tea
Column:
Venusil Unisol C18 （4.6×250 mm，5 μm）
Part No.:
UO952505-0
Mobile phase: 0.02%H3PO4: MeOH=81:19
Detection:
UV 278nm
Flow rate:
1 mL/min
Temperature: 40℃
10.6 Saccharin Sodium in Milk Powder
Sample:
Benzoic acid, 2,4-Hexadienoic acid,
Sodium
saccharine, milk powder
Column:
Venusil Unisol C18 （4.6×250 mm，5 μm）
Part No:
UO952505-0
Mobile phase:MeOH:CH3COONH4 (0.02 M) =5:95
Detection:
UV 230nm
Flow rate:
1 mL/min
Temperature: 30℃
10.7 DHA in the Health Food
Column:
Unisol C18 （4.6×250 mm，5 μm）
Part No.:
UO951505-0
Mobile phase:MeOH:Water =80:20
Detection:
UV 254nm
Flow rate:
1 mL/min
Injection:
10 μL
10.8 Tartrazine in Food
Column:
Unisol C18（ 4.6×250 mm，5 μm）
Part No.:
UO951505-0
Mobile phase: A: Ammonium acetate
buffer（pH 4.0）;B: MeOH
Gradient:
35%B to 50%B in 5 min.
Detection:
UV 254nm
Flow rate:
1 mL/min
Injection:
10 μL
Column:
Part No.:
VS952505-0
10.9 Melamine in Feed
Mobile phase: 10mM Citric acid+10mM Perfluorooctane
sulfonate( pH 3.0) :ACN=85:15
Detection:
UV 240nm
Flow rate:
1 mL/min
Injection:
10 μL
Temperature: 40 ℃
10.10 Tetrodotoxin（TTX）
Column:
Part No.:
VS952505-0
Mobile phase: 0.02% H3PO4:MeOH=40:60
Detection:
UV 200nm
Flow rate:
0.5mL/min
Temperature: 25 ℃
10.11 Baicalin
Column:
Venusil ASB C18, (4.6×150 mm，5 μm)
Part No.:
VA951505-0
Mobile phase: MeOH : 1% acetic acid =50:50
Detection:
UV 274nm
Flow rate:
1 mL/min
Injection:
5 μL
APPENDIX
Due to interactions between the stationary phase and sample components, HPLC columns may
occasionally require cleaning or regeneration. The following conditions apply to silica-based columns. Flow
rates should be 1/5 - 1/2 of the typical flow rate.
To estimate the column volume, use the following equation:
V = column volume in mL
V=pr2 / L
r = column radius in cm
L = column length in cm
Rinse with 10 column volumes each of: Hexane, Methylene, Chloride,
UNBONDED SILICA COLUMNS (SILICA)
Isopropanol, Methylene Chloride. Mobile phase: Flush column with 30mL
2.5% 2,2-dimethoxy- propane and 2.5% glacial acetic acid in hexane
REVERSED PHASE COLUMNS (C18, C8,
Rinse with 10 column volumes of: 95% Water/5% Acetonitrile (for buffer
C4, C2, C1, PHENYL, CN, NH2)
removal) followed by 95% Acetonitrile/5% Water mobile phase
Rinse with 20 column volumes of mobile phase with buffer removed run
REVERSED PHASE PROTEIN/PEPTIDE
gradient (2x): A) 0.1% Aqueous TFA in Water B) 0.1% TFA in
COLUMNS (C18, C8, C5, C4, PHENYL)
Acetonitrile/Isopropanol (1:2) 25% B 100% B for 30 minutes Equilibrate
with 10 column volumes of mobile phase.
Rinse with 10 column vol umes each of : Chloroform,Isopropanol,
Methylene chloride, mobile phase. Exception: Recommended for cleaning
BONDED NORMAL PHASE COLUMNS
Luna Amino when used in reversed phase mode: 1. Wash with at least 30
(CN, NH2, DIOL )
column volumes of Sodium Hydroxide (pH 11.0) 2. Flush with at least 30
column volumes of Water (HPLC grade) 3. Re-equilibrate to Mobile phase
conditions.
(300 X 7.8mm size columns) Rinse with 5 column volumes of: 0.1M
GFC/SEC COLUMNS FOR PROTEINS
Phosphate buffer pH 3.0. For strongly retained proteins: Run 100% Water to
100% Acetonitrile to 100% Water over 60 minutes or wash with
5 column volumes of SDS or 6M Guanidine Thiocyanate or 10% DMSO
Rinse with 10 column volumes of:
500mM Phosphate buffer pH 7 10% Acetic acid (Aq) 5 Column Volumes of
Water
10 Column Volumes of Phosphate buffer pH 7 5 Column Volumes of Water
ION-EXCHANGE COLUMNS
10 Column Volumes of Methanol
(SAX, SCX, NH2, DEAE)
10 Column Volumes of Water
For protein removal Follow the above procedure with this exception:
Substitute 10 column volumes of Methanol with 10 column volumes of 5M
Urea or 5M
Guanidine Thiocyanate
The Methods of Maintaining Good Column Lifetime and Performance
z
Inject only well-prepared (filtration, liquid/liquid extraction, SPE) clean samples
z
Minimize pressure surge; avoid mechanical and thermo shock.
z
Use guard columns or on-line filtration.
z
Flush columns frequently using an appropriate program
z
Remove unstable and strongly retained components of no-interest from samples
z
Use low pH (1-6) mobile phase if possible.
z
Use organic buffer when operating at medium to high pH (6-10)
z
Avoid elevated temperature unless it is necessary
z
Add 200 ppm sodium azide in aqueous mobile phase to suppress the growth of the bacteria
z
Wash out all buffer salts and store the columns in high organic solution for overnight or long time
storage
SPE Columns and Wellplates
z
Cleanert SPE Columns, Wellplates and Media
z
z
The Development of SPE Method ----------------------------------------------------- 103
Applications ---------------------------------------------------------------------------------- 106
Cross-reference Table --------------------------------------------------------------------- 114
z
----------------------------------Cleanert Plus SPE Columns and 96 Wellplates-NEW
-------------------------------OMM Technology for SPE-NEW
------------------------------------------------SLE Technology and Sorbents
------------------------------------------------------------Bonded Silica SPE
--------------------------------------------------------------------------Non-silica Adsorption Sorbent
------------------------------------------------------------Mixed and Layered Phases
----------------------------------------------------------------Specialized Phases
-------------------------------------------------------------------------Cleanert Pre-IC
------------------------------------------------------------------------------
078
080
082
089
091
097
099
100
102
Cleanert SPE Columns, Wellplates and Media
Agela Technologies provides a full line of SPE sorbents, columns and wellplates.
OMM SPE Products (Optimized Molecular Modification)- PEP Plus, PEP, PAX, PCX, PWAX, PWCX and
PS are all based on polystyrene/divinylbenzene while each phase has different functionality and unique
selectivity. They are highly recommended for the extraction of a wide range of compounds in
pharmaceutical, agricultural, food, and environmental industries. PEP and PEP Plus are good replacement
of Water Oasis® HLB. Average particle diameter: 35-70μm; Average pore size: 80Å; Specific surface area:
600m2/g.
SLE(Solid supported Liquid/liquid Extraction) columns and well-plates
Specially treated diatomite materials are packed in columns and well plates. The liquid/liquid extraction is
run on the surface of the materials, which is easily automated in parallel and time-saving.
Bonded silica based SPE column
C18, C18-N, C8, NH2, COOH, Silica, PSA, PRS, SCX, SAX; All of them are made of high quality and low
metal silica particles. Using the special surface modification methodology, the activity of silica surface is
reduced largely, which in turn will reduce the tailing of compounds and will ensure high recovery and good
reproducibility.
Characteristics of silica particles:
Average particle diameter: 50μm
Average pore size: 60Å
Volume of pore: 0.8cm2/g
Specific surface area: 480m2/g
Surface of silica: made by special surface modification
Non-silica adsorption phase columns-Florisil, PestiCarb, Alumina(N, A, B). They are commonly used to
remove polar interference from non-polar samples. Cleanert adsorption sorbents have high purity, high
recovery and good reproducibility. They are widely used in sample preparation for environmental and food
analysis.
Mixed and layered phases-C8/SCX, PestiCarb/NH2, PestiCarb/PSA
Specialized phases-SUL-5, DNPH-Silica,HXN
New Product!
Cleanert Plus SPE Columns and 96 Wellplates
Easier, faster and more reliable!
Description
Cleanert Plus SPE products use the particles with narrowly controlled size distribution, a novel frit material
& design, unique pre-activation process on reverse phase, and a pre-drying on normal phase columns.
Benefits:
Stable flow rate, clogging free:
The usual problem of cartridge clogging caused by the sticky and slimy samples can be completely
eliminated by using the PEP Plus product line, which makes the sample preparation easier, faster, and more
reliable than ever.
No activation step is needed, saving 1/3 of work load and time:
Using the new Cleanert PEP Plus can save you 1/3 of your work load eliminating the pre-activation steps.
PEP Plus does not suffer from vacuum-drying effect, and has balanced adsorption for a wide range of
analytes. PEP Plus is the first choice for reverse phase SPE methods development.
More consistent performance from normal phase by pre-drying:
The new Cleanert Florisil Plus, Cleanert Alumina Plus, Cleanert Pesticarb Plus , Cleanert Silica Plus and
Cleanert NH2 Plus will give an unprecedented performance consistency owing to the use of special frits,
narrow particle distribution, and pre-drying features.
Faster and reliable alternative to liquid/liquid extraction:
The new Cleanert SLE Plus Solid Supported Liquid/Liquid Extraction columns and plates use specially
treated diatomite materials to run liquid/liquid extraction on solid supports. Cleanert SLE Plus products are
designed for automation and parallel process for time saving.
Application Examples:
SPE-HPLC Analysis of Terramycin in Serum of Mouse using Cleanert PEP Plus
method and the result of Cleanert PEP 60mg/3mL
method and the result of Cleanert PEP Plus 60 mg/3mL
a. Wash with 2mL methanol, 2mL water
Preconditioned
12 mins by gravity
b. Load 2mL sample solution(the mouse serum which
contains 10ppm terramycin); 15 mins by gravity
0 mins
Load 2mL sample solution(the mouse serum which contains 10ppm
terramycin); 4 mins by gravity
c. Wash with 3mL water, then drying the column fully
Wash with 3mL water, then drying the column fully
d. Elute terramycin with 3mL methanol
Elute terramycin with 3mL methanol
e. Evaporate the elution by nitrogen at room temperature;
Evaporate the elution by nitrogen at room temperature; dissolve
dissolve the residue with 2mL mobile phase
f. Recovery: 83.2%
the residue with 2mL mobile phase
Recovery: 81.4%
Cleanert PEP Plus
Material
Sorbent
Vol
Tubes/box
Cat.
Number
A hydrophilic and
lipophilic balanced
material, made of
polydivinyl benzene
functionalized with a
polar group
30mg
1mL
100
PE0301-P
50mg
1mL
100
PE0501-P
60mg
3mL
50
PE0603-P
100mg
3mL
50
PE1003-P
200mg
6mL
30
PE2006-P
500mg
6mL
30
PE5006-P
15mg/well
2mL
96-wellplate
PE0152-WP
30mg/well
2mL
96-wellplate
PE0302-WP
50mg/well
2mL
96-wellplate
PE0502-WP
Cleanert Florisil Plus
Description
The new Cleanert Florisol Plus SPE products features a special frit design which made these columns
clogging-free. The SPE columns also offer an unique “pre-drying” function to samples by removing water
that is contained in the samples. These columns have unprecedented consist performance.
Average particle diameter: 80-100μm
Pore size: 80Å
Specific surface area: 250-300m2/g
Material
Sodium
sulfate/Florisil
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
FS1001-P
200mg
3mL
100
FS2003-P
500mg
3mL
50
FS5003-P
500mg
6mL
50
FS5006-P
1g
6mL
30
FS0003-P
2g
10mL
15
FS2000-P
100mg/well
2mL
96-wellplate
FS1002-WP
Cleanert Silica Plus
Description
The new Cleanert Silical Plus SPE products features a special frit design which made these columns
clogging-free. The SPE columns also offer an unique “pre-drying” function to samples by removing water
that is contained in the samples. These columns have unprecedented consist performance.
Average particle diameter: 40-60μm
Pore size: 60Å
2
Specific surface area: 480m /g
Material
Sodium
sulfate/Silica
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
SI1001-P
200mg
3mL
100
SI2003-P
500mg
3mL
50
SI5003-P
500mg
6mL
50
SI5006-P
1g
6mL
30
SI0006-P
50mg/well
2mL
96-wellplate
SI0502-WP
100mg/well
2mL
96-wellplate
SI1002-WP
OMM Technology (Optimized Molecular
Modification) for SPE
New Product!
Agela’ s SPE products have been developed based on a thorough understanding of the science, e.g.,
interactive natures of chemical molecules. Our SPE products can thus better meet customer’ s needs. Our
R&D results demonstrated that the adsorption/desorption property of the polymeric SPE materials is
regulated by the types of the functional groups and the degree of substitution of the surface modification.
In general, modification with electron donor groups will help to retain the electron-deficient molecules, while
modification with electronwithdrawing groups will prefer to retain the electron-rich molecules. Different SPE
materials have been developed by incorporating proper types of functional groups and degree of
substitutions on the surface, and thus providing optimized and balanced performance for all types of
molecules.
Cleanert PEP (Polar Enhanced Polymer) is a polymer based SPE sorbent which consists of hydrophilically
modified divinyl benzene. Cleanert PEP polymer sorbent is water-wettable and it does not suffer from
column drying effect. It thus offers consistent performance due to proper wetting capability which is
especially important for 96-well plates. It also has high retention for polar compounds and high capacity for a
wide spectrum of analytes.
Good Water-Wettability
The Cleanet PEP (polar polymer) sorbent is a unique hydrophilic-lipophilic balance material. It provides
excellent wettability since the sorbent hydrophobic surface is highly modified with polar functional groups.
The SPE mechanism follows the reverse phase separation principle.
Effect of Drying on Recovery - PEP Versus C18 Sorbents
High Retention for Polar Compounds
The electron donating and withdrawing functionalities of the sorbent allows it to have enhanced retention for
polar compounds.
Consistent Performance for Acidic, Neutral and Basic Compounds
PEP sorbents are water-wettable, maintaining high retention and high capacity for a wide spectrum of
analytes, even when the SPE column runs dry.
High Capacity
The PEP sorbent has 2-3x more surface area and shows a dramatic increase of sample capacity compared
to silica-based C18. The volume of elution solvents can be reduced, and subsequently the total operation
time (including solvent evaporation) will be shortened
Excellent Batch-to-Batch Reproducibility
The OMM technology offers consistent surface modifications and thus batch-to-batch reproducibility.
Multiple batches of PEP, PCX, and PAX have been successfully used for various compounds with
consistent results.
PEP Plus (Pre-conditioned and Clog Resistant)
Description
The PEP Plus columns are packed with high quality PEP sorbents. The sorbents contain the high surface
area polydivinylbenzene spherical particles that are modified with polar groups to offer a balanced
hydrophilicity and hydrophobicity. The characteristics of the sorbent are: water-wettable (don’ t suffer from
undesirable drying effect), wide usable pH range (1-14), high retention for polar compounds, and high
capacity.
PEP Plus has a novel frit material & design, and it has undergone a pre-activation process step during
manufacturing. This eliminates the need for an activation step prior to use thus reducing working load by
~30%.more than 1/3 work load can be saved.
Particle Characteristics
2
Polar and spherica l particles: Average particle size: 40μm; Average pore size: 70Å ;Special surface area: 600m /g
Material
Cleanert
PEP
Plus
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
PE0301-P
60mg
3mL
50
PE0603-P
100mg
3mL
50
PE1003-P
200mg
6mL
30
PE2006-P
500mg
6mL
30
PE5006-P
30mg/well
2mL
96-wellplate
PE0302-WP
50mg/well
2mL
96-wellplate
PE0502-WP
10g
PE0010-P
100g
PE0100-P
PEP(Polar Polymers)
Description:PEP (polar polymers, alternative to Oasis HLB)
PEP is made of polydivinylbenzene on which the surface is functionalized with vinyl prolidone. The material
has a balanced hydrophilic and hydrophobic property and can be used in the entire pH range of 1-14.
PEP can be used to extract a variety of polar and non-polar compounds. Some highly hydrophilic
compounds which have little retention on C18 columns, such as chlorinated phenols, phosphate esters and
drug metabolites, can be effectively retained on PEP
2
Functionalized polymer sorbents; Average particle size: 35μm.Average pore size: 80Å; Surface area: 600m /g
Material
Cleanert
PEP
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
PE0301
60mg
3mL
50
PE0603
100mg
3mL
50
PE1003
200mg
6mL
30
PE2006
500mg
6mL
30
PE5006
30mg/well
2mL
96-wellplate
PE0302-W
50mg/well
2mL
96-wellplate
PE0502-W
10g
PE0010
100g
PE0100
PAX(RP/Strong Anion Exchange)
Description
It is designed to overcome the limitations of traditional silica based mixed-mode SPE sorbents such as
C18/SAX. It is a RP/strong anionexchange mixed mode polystyrene/divinylbenzene sorbent, stable from pH
0-14.
Based on functioalized polystyrene/divinylbenzene;
Average particle diameter: 40μm; Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area:
2
600m /g.
Material
Cleanert PAX
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
AX0301
60mg
3mL
50
AX0603
100mg
3mL
50
AX1003
200mg
6mL
30
AX2006
500mg
6mL
30
AX5006
30mg/well
2mL
96-wellplate
AX0302-W
50mg/well
2mL
96-wellplate
AX0502-W
10g
AX0010
100g
AX0100
PCX(RP/Strong Cation Exchange)
Description
PCX is a mixed-mode, strong cation exchange sorbent. It has reverse-phase and anion-exchange dual functionality. It
high surface area and a wide usable pH range of 0-14.
Based on Functionalized polystyrene/divinylbenzene;
2
Average particle diameter: 40μm; Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m /g.
Material
Cleanert PCX
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
CX0301
60mg
3mL
50
CX0603
100mg
3mL
50
CX1003
200mg
6mL
30
CX2006
500mg
6mL
30
CX5006
30mg/well
2mL
96-wellplate
CX0302-W
50mg/well
2mL
96-wellplate
CX0502-W
10g
CX0010
100g
CX0100
PWAX(RP/Weak Anion Exchange)
Description
Cleanert PWAX provides the dual modes of retention, weak anion exchange and reverse phase on a stable
polymer sorbent , which improves the retention for acidic analytes.
Based on partially functionalized aminopolystyrene/divinylbenzene; Average particle diameter: 40μm;
2/
Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m g.
Material
Cleanert PWAX
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
WAX0301
60mg
3mL
50
WAX0603
100mg
3mL
50
WAX1003
200mg
6mL
30
WAX2006
500mg
6mL
30
WAX5006
30mg/well
2mL
96-wellplate
WAX0302-W
50mg/well
2mL
96-wellplate
WAX0502-W
10g
WAX0010
100g
WAX0100
PWCX(RP/Weak Cation Exchange)
Description
Cleanert PWCX provides the dual modes of retention, weak cation exchange and reverse phase on a stable
polymer sorbent, which improves the retention for basic analytes.
Based on partially functionalized polystyrene/divinylbenzene; Average particle diameter: 40μm; Average
pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g.
Material
Cleanert PWCX
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
WCX0301
60mg
3mL
50
WCX0603
100mg
3mL
50
WCX1003
200mg
6mL
30
WCX2006
500mg
6mL
30
WCX5006
30mg/well
2mL
96-wellplate
WCX0302-W
50mg/well
2mL
96-wellplate
WCX0502-W
10g
WCX0010
100g
WCX0100
PEP-ED
Description
PEP-ED is made of the polydivinylbenzene having a surface functionalized with a electron donating group.
They can be used in the entire pH 1-14. The surface has a balanced hydrophilic and hydrophobic property
and can extract a variety of polar and non-polar compounds, especially having electron-deficient structures .
Adsorption sorbents; Average particle size: 35μm.Average pore size: 80Å; Surface area: 600m2/g
Material
Cleanert PEP ED
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
PE0301-E
60mg
3mL
50
PE0603-E
100mg
3mL
50
PE1003-E
200mg
6mL
30
PE2006-E
500mg
6mL
30
PE5006-E
30mg/well
2mL
96-wellplate
PE0302-WE
50mg/well
2mL
96-wellplate
PE0502-WE
10g
PE0010-E
100g
PE0100-E
PEP-ER
Description
PEP-ER is made of the polydivinylbenzene having a surface functionalized with a electron withdrawing
group . They can be used in the entire pH 1-14. The surface has a balanced hydrophilic and hydrophobic
property and can extract a variety of polar and non-polar compounds, especially having a electron-rich
structure
Adsorption sorbents; Average particle size: 35μm.Average pore size: 80Å; Surface area: 600m2/g
Material
Cleanert PEP ER
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
PE0301-R
60mg
3mL
50
PE0603-R
100mg
3mL
50
PE1003-R
200mg
6mL
30
PE2006-R
500mg
6mL
30
PE5006-R
30mg/well
2mL
96-wellplate
PE0302-WR
50mg/well
2mL
96-wellplate
PE0502-WR
10g
PE0010-R
100g
PE0100-R
PS
Description
PS is made of non-substituted polydivinylbenzene. It has larger surface areas (>600m2/g.) and thus greater
capacity than reverse phase bonded silica.PS can be used for the extraction of non-polar and polar
compounds.
Based on polystyrene/divinylbenzene; Average particle diameter: 40μm;
2
Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m /g.
Material
Cleanert PS
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
PS0301
60mg
3mL
50
PS0603
100mg
3mL
50
PS1003
200mg
6mL
30
PS2006
500mg
6mL
30
PS5006
30mg/well
2mL
96-wellplate
PS0302-W
50mg/well
2mL
96-wellplate
PS0502-W
10g
PS0010
100g
PS0100
SLE (Solid Supported Liquid/liquid Extraction)
Technology and Sorbents
This technique utilizes a layer of solid material, which is a type of modified diatomaceous earth, as a support
for liquid/liquid extraction.
Mechanism
z
A modified form of diatomaceous earth is packed in a cartridge.
z
Biological fluid can be applied to the cartridge using gravity flow.
z
The aqueous sample is deposited as a thin film on the hydrophilic surface.
z
The analytes are extracted with a water immiscible organic solvent
SLE
Description
Solid supported liquid/liquid Extraction columns and plates use specially treated diatomiteous materials as a
solid support for liquid/liquid extractions. The SPE procedures can be easily automated.
The General Method Includes:
1. Load an aqueous sample into the column by gravity or a soft vacuum
2. Apply one or a multiple organic solvents by gravity or well-controlled vacuum
3. Vacuum the organic solution which contains the analyte out from bottom of the columns or plates, collect
the solution
4. Concentrate the collected solution
Material
Special treated
diatomite
Sorbent
Vol
Tubes/box
Cat. Number
200mg
3mL
100
SL2003
500mg
3mL
50
SL5003
500mg
6mL
50
SL5006
1g
6mL
30
SL0006
14g
40mL
10
SL1400
300mg/well
2mL
96-wellplate
SL3002-W
500mg/well
2mL
96-wellplate
SL5002-W
SLE Plus
Description
Solid Supported Liquid/Liquid Extraction columns and plates. They use the specially treated diatomite
materials to run liquid/liquid extractionson the solid supports, which are easily automated in parallel and time
saving.
Compared with SLE, the SLE Plus has a special frit design which keeps the columns clogging-free, even for
very nasty samples.
The general method includes:
1. Load an aqueous sample into the column by gravity or a soft vacuum
2. Apply one or a multiple organic solvents by gravity or well-controlled vacuum
3. Vacuum the organic solution which contains the analyte out from bottom of the columns or plates, collect
the solution
4. Concentrate the collected solution
Material
Special treated
diatomite
Sorbent
Vol
Tubes/box
Cat. Number
200mg
3mL
100
SL2003-P
500mg
3mL
50
SL5003-P
500mg
3mL
50
SL5006-P
1g
6mL
30
SL0006-P
14g
40mL
10
SL1400-P
300mg/well
2mL
96-wellplate
SL3002-WP
500mg/well
2mL
96-wellplate
SL5002-WP
Bonded Silica SPE
ODS C18(End-capped)
Description
ODS C18 products columns and plates are packed with reverse phase,
octadecylsilane bonded silica sorbents. The sorbent is double endcapped
and has a high bonding density (%C>17). These columns can be used as a
replacement for BondElute C18 and Super clean ENVI C18. These products
can be used for desalting biomolecules, such as proteins and DNAs.
Based on silica; C%: 18-19%; Average particle diameter: 50μm;
2
2
Average pore size: 60Å; Volume of pore: 0.8cm /g;Specific surface area: 480 /g
Material
Cleanert
ODS(C18,end-capped)
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
181001
200mg
3mL
50
182003
500mg
3mL
50
185003
500mg
6mL
30
185006
1g
6mL
30
180006
50mg/well
2mL
96-wellplate
180502-W
100mg/well
2mL
96-wellplate
181002-W
10g
180010
100g
18010
ODS C18-N(Non-end-capped)
Description
ODS C18-N products have silica based reverse phase sorbents bonded with octadecylsilane without endcapping. The
extra silanol residue of the sorbent provides additional polar interactions associated with surface silanol groups which
enhance the retention of basic compounds. These columns are similar to
Agilent AccuBond C18 and BondElute C18 OH.
2
Average pore size: 60Å; Volume of pore: 0.8cm /g;Specific surface area:
2
480m /g
Material
Cleanert
(C18,Non-end-capped)
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
181001-N
200mg
3mL
50
182003-N
500mg
3mL
50
185003-N
500mg
6mL
30
185006-N
1g
6mL
30
180006-N
50mg/well
2mL
96-wellplate
180502-N-W
100mg/well
2mL
96-wellplate
181002-N-W
10g
180010-N
100g
180100-N
C8 (Octyl)
Description
The property of C8 products is similar to ODS C18 products. However, this sorbent is slightly less retentive
than C18, which facilitates the elution of more hydrophobic
substance. C8 is successfully used for the extraction of both
water-soluble and fat-soluble vitamins from serum, as well as the
desalting of biological macromolecules.
2
Average pore size: 60Å; Volume of pore: 0.8cm /g;Specific
surface area: 480m2/g
Material
Cleanert C8
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
81001
200mg
3mL
50
82003
500mg
3mL
50
85003
500mg
6mL
30
85006
1g
6mL
30
80006
50mg/well
2mL
96-wellplate
080502-W
100mg/well
2mL
96-wellplate
081002-W
10g
80010
100g
80100
CN (Cyanopropyl)
Description
Cyano(CN) SPE have silica based sorbents bonded with
cyanopropyl functional group. This polar sorbent exhibits both
polar and non-polar interactions. It can be used for extraction
of both polar and non-polar molecules in either normal phase
reverse phase mode.
2
2
Average pore size: 60Å; Volume of pore: 0.8cm /g;Specific surface area: 480m /g
Material
Cleanert CN
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
CN1001
200mg
3mL
50
CN2003
500mg
3mL
50
CN5003
500mg
6mL
30
CN5006
1g
6mL
30
CN0006
50mg/well
2mL
96-wellplate
CN0502-W
100mg/well
2mL
96-wellplate
CN1002-W
10g
CN0010
100g
CN0100
NH2 (Aminopropyl)
Description
NH2 SPE products have silica based sorbents bonded with aminopropyl
funtional group. This sorbent can be used in either normal phase or
reverse phase mode. It retains the analytes either by a polar adsorption
(from non-polar solution) or by weak anion exchange (from aqueous
solution). pKa=9.8.
Based on silica; Average particle diameter: 50μm;
2
2
Material
Cleanert NH2
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
NH1001
200mg
3mL
50
NH2003
500mg
3mL
50
NH5003
500mg
6mL
30
NH5006
1g
6mL
30
NH0006
50mg/well
2mL
96-wellplate
NH0502-W
100mg/well
2mL
96-wellplate
NH1002-W
10g
NH0010
100g
NH0100
PSA 〔(N-aminoethyl) Aminopropyl〕
Description
PSA SPE is similar to Cleanert-NH2. PSA has two amino groups with
pKa=10.1 and 10.9 respectively. This sorbent is an anion exchanger
slightly stronger than NH2. It can be used for the extraction of metal ions
by chelating interactions. It is also commonly used to remove organic
acids, pigments an metal ions from organic samples such as vegetables
and fruits.
Based on silica; Average particle diameter: 50μm;
2
2
Material
Cleanert PSA
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
PA1001
200mg
3mL
50
PA2003
500mg
3mL
50
PA5003
500mg
6mL
30
PA5006
1g
6mL
30
PA0006
50mg/well
2mL
96-wellplate
PA0502-W
100mg/well
2mL
96-wellplate
PA1002-W
10g
PA0010
100g
PA0100
SAX (Strong Anion Exchanger)
Description
SAX SPE products have silica based sorbents bonded with a
quaternary amine. This strong anion exchanger is used to
extract compounds capable of carrying a negative charge from
both aqueous and non-aqueous solutions. They are ideally suit
to extraction of weak acids and desalting of biological
macromolecules, like BondElute-SAX.
Based on silica; C%: 9-10%; Average particle diameter: 50μm; Average pore size: 60Å;
2
2
Volume of pore: 0.8cm /g;Specific surface area: 480m /g The ion exchange degree: 0.5meq/g.
Material
Cleanert SAX
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
SA1001
200mg
3mL
50
SA2003
500mg
3mL
50
SA5003
500mg
6mL
30
SA5006
1g
6mL
30
SA0006
50mg/well
2mL
96-wellplate
SA0502-W
100mg/well
2mL
96-wellplate
SA1002-W
10g
SA0010
100g
SA0100
COOH (Weak Cation Exchanger)
Description
COOH SPE products consist of a propane carboxylic acid on the
inner silica surface. The pKa of the carboxylic acid group is
approximately 3.8. It is a useful orbent for quaternary ammonium salt
and other strong cations.
Average pore size: 60Å; Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Material
Cleanert COOH
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
CH1001
200mg
3mL
50
CH2003
500mg
3mL
50
CH5003
500mg
6mL
30
CH5006
1g
6mL
30
CH0006
50mg/well
2mL
96-wellplate
CH0502-W
100mg/well
2mL
96-wellplate
CH1002-W
10g
CH0010
100g
CH0100
PRS (Propane Sulfonic Acid)
Description
PRS SPE sorbent is a silica gel based strong cation exchanger.
This sorbent, consisting of a propane sulfonic acid, has slightly
less exchange caoability than SCX. It can be applied to the
extraction of weak cations, such as pyridine, with high recovery.
Based on silica; Average particle diameter: 50μm; Average pore
2
2
size: 60Å;Volume of pore: 0.8cm /g;Specific surface area: 480m /g The ion exchange degree: 0.3meq/g.
Material
Cleanert PRS
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
PR1001
200mg
3mL
50
PR2003
500mg
3mL
50
PR5003
500mg
6mL
30
PR5006
1g
6mL
30
PR0006
50mg/well
2mL
96-wellplate
PR0502-W
100mg/well
2mL
96-wellplate
PR1002-W
10g
PR0010
100g
PR0100
SCX (Strong Cation Exchanger)
Description
SCX products are strong cation exchangers based on silica gel,
with benzene sulfonic acid. The sorbent is used to extract
positively charged basic compounds or remove the salt from
biological samples. It can also be mixed with C18 sorbent to
extract the organic bases.
Based on silica; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g The ion exchange degree: 0.5meq/g.
Material
Cleanert SCX
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
SC1001
200mg
3mL
50
SC2003
500mg
3mL
50
SC5003
500mg
6mL
30
SC5006
1g
6mL
30
SC0006
50mg/well
2mL
96-wellplate
SC0502-W
100mg/well
2mL
96-wellplate
SC1002-W
10g
SC0010
100g
SC0100
Silica
Description
Silica SPE product has unbonded, activated irregular silica as the
sorbent. This sorbent exhibits high polar interaction and is used for the
extraction of weak-polar or non-polar compound, such as oil. In addition,
the silanol groups are ionzable at intermediate pH, so it can be used as a
weak cation exchanger.
Based on silica; Average particle diameter: 50μm; Average pore size: 60Å;
2
2
Volume of pore: 0.8cm /g;Specific surface area: 480m /g
Material
Cleanert Silica
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
SI1001
200mg
3mL
50
SI2003
500mg
3mL
50
SI5003
500mg
6mL
30
SI5006
1g
6mL
30
SI0006
50mg/well
2mL
96-wellplate
SI0502-W
100mg/well
2mL
96-wellplate
SI1002-W
10g
SI0010
100g
SI0100
Diol
Description
Silica based dihydroxy SPE. It is used to extract polar analytes from
non-polar solutions. It is a neutral sorbent and extracts compounds by
forming hydrogen bonding or polar-polar interaction. As an example, it
can be used to extract THC.
2/
2/
Average pore size: 60Å;Volume of pore: 0.8cm g;Specific surface area: 480m /g
Material
Cleanert Diol
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
DI1001
200mg
3mL
50
DI2003
500mg
3mL
50
DI5003
500mg
6mL
30
DI5006
1g
6mL
30
DI0006
50mg/well
2mL
96-wellplate
DI0502-W
100mg/well
2mL
96-wellplate
DI1002-W
10g
DI0010
100g
DI0100
Non-silica Adsorption Sorbent
Florisil (Magnesia Silica)
Description
PestiCarb is made of graphitized carbon by a distinct surface modification process, and has been used for
sample cleanup in pesticide residues in plant or animal tissues. This sorbent can effectively reduce the
background noise and increase the sensitivity, the functions of which are similar to Supelco Envicarb.
Adsorption sorbents; Average particle diameter: 80-100μm (40-60μm optional);
2
Average pore size: 80Å; Specific surface area: 291m /g
Material
Cleanert Florisil
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
FS1001
200mg
3mL
50
FS2003
500mg
3mL
50
FS5003
500mg
6mL
30
FS5006
1g
6mL
30
FS0006
50mg/well
2mL
96-wellplate
FS0502-W
100mg/well
2mL
96-wellplate
FS1002-W
10g
FS0010
100g
FS0100
PestiCarb (Graphitized Carbon)
Description
PestiCarb is made of graphitized carbon by a distinct surface modification process, and has been used for
sample cleanup in pesticide residues in plants or animal tissues. This sorbent can effectively reduce the
background noise and increase the sensitivity, the functions of which are similar to Supelco Envicarb.
Adsorption sorbents; Average particle size: 120-400 mesh.
Material
Cleanert PestiCarb
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
PC1001
200mg
3mL
50
PC2003
500mg
3mL
50
PC5003
500mg
6mL
30
PC5006
1g
6mL
30
PC0006
50mg/well
2mL
96-wellplate
PC0502-W
100mg/well
2mL
96-wellplate
PC1002-W
10g
PC0010
100g
PC0100
Alumina N (Aluminium Oxide;Neutral)
Description
Alumina N sorbents(pH=7.5) can adsorb molecules by interaction with the aluminum metal center. The
neutralized surface allows interaction with compounds whose heteroatoms are electronegative(e.g.N,S,P)
or with an electron-rich ,highly aromatic structure.
Adsorption sorbents;
Average particle size: 150 mesh; Average pore size: 58Å;
Material
Cleanert
Alumina N
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
AL1001-N
200mg
3mL
50
AL2003-N
500mg
3mL
50
AL5003-N
500mg
6mL
30
AL5006-N
1g
6mL
30
AL0006-N
50mg/well
2mL
96-wellplate
AL0502-N-W
100mg/well
2mL
96-wellplate
AL1002-N-W
10g
AL0010-N
100g
AL0100-N
Alumina A (Aluminium Oxide;Acidic)
Description
Alumina A sorbents(pH=4.5) can be used as strong polar absorbents or mild cation exchangers. This
sorbent is processed with a special deactivation procedure which ensures high analytes recovery.
Average particle size: 150 mesh; Average pore size: 58Å;
Material
Cleanert
Alumina A
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
AL1001-A
200mg
3mL
50
AL2003-A
500mg
3mL
50
AL5003-A
500mg
6mL
30
AL5006-A
1g
6mL
30
AL0006-A
50mg/well
2mL
96-wellplate
AL0502-A-W
100mg/well
2mL
96-wellplate
AL1002-A-W
10g
AL0010-A
100g
AL0100-A
Alumina B (Aluminium Oxide; Basic)
Description
Alumina B products(pH=10) can be used to remove organic acids and phenols in the sample matrix. They
have been pre-treated by special deactivation to ensure high analytes recovery.
Average particle size: 150 mesh; Average pore size: 58Å.
Material
Cleanert
Alumina B
Sorbent
Vol
Tubes/box
Cat. Number
100mg
1mL
100
AL1001-B
200mg
3mL
50
AL2003-B
500mg
3mL
50
AL5003-B
500mg
6mL
30
AL5006-B
1g
6mL
30
AL0006-B
50mg/well
2mL
96-wellplate
AL0502-B-W
100mg/well
2mL
96-wellplate
AL1002-B-W
10g
AL0010-B
100g
AL0100-B
Mixed and Layered Phases
PestiCarb/NH2
Description
PestiCarb/NH2 SPE column is packed of 500mg PestiCarb and 500mg NH2.It has been used wildly in
analysis of pesticides residue, esp. for the JapanesePositive List System. It can be used in pesticide residue
analysis, coloring matter,fatty acid and hydroxybenzene.
See Pesticarb and Cleanert NH2
Material
Sorbent
Vol
Tubes/box
Cat. Number
Cleanert PestiCarb/NH2
500mg/500mg
6ml
30
PN0006
C8/SCX
Description
Mixed mode SPE based on silica of C8 and strong cation-exchange. It is usually used for the extraction of
basic drugs from urine or blood
See C8 and SCX
Material
Sorbent
Vol
Tubes/box
Cat. Number
50mg
3mL
50
CS0503
130mg
3mL
50
CS1303
300mg
3mL
50
CS3003
500mg
6mL
30
CS5006
1g
6mL
30
CS0006
50mg/well
2mL
96-wellplate
CS0502-W
100mg/well
2mL
96-wellplate
CS1002-W
Cleanert C8/SCX
10g
CS0010
100g
CS0100
Specialized Phases
HXN(Mid Polar Polymers Specially for Sulfonyl Urea Samples)
Description
HXN is also made of polydivinylbenzene having surface modified with vinylpyrrolidone. This sorbent is
specially designed to extract sulfonyl ureas from water and soil at ppb level. It is less polar than PEP and
can also be used to extract, enrich and clean up samples from mid polar to high polar compounds.
Based on polystyrene/divinylbenzene;Average particle diameter: 40μm;
Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g
Material
Cleanert HXN
Sorbent
Vol
Tubes/box
Cat. Number
30mg
1mL
100
HX0301
60mg
3mL
50
HX0603
100mg
3mL
50
HX1003
200mg
6mL
30
HX2006
500mg
6mL
30
HX5006
30mg/well
2mL
96-wellplate
HX0302-W
50mg/well
2mL
96-wellplate
HX0502-W
10g
HX0010
100g
HX0100
SUL-5(Specific Columns for Sulfonamides)
Description
SUL-5（specific columns for sulfonamides）is specially designed for the extraction of five sulfonamides
(SM2,SMM,SMZ,SDM.SQ) in pork.
Adsorption sorbents.
Material
Sorbent
Vol
Tubes/box
Cat. Number
Cleanert SUL-5
2g
10ml
100
SUL-5
Cleanert Pre-IC:
Sample Clean-up Cartridges for the Ion Chromatography
Description
The sample preparation is necessary not only for HPLC but also for IC.
The demands for sample preparation of IC led to a development of a new SPE product family, which we
named“Cleanert Pre-IC”, and theycan perform excellently for sample preparation of IC thanks to their
sorbent of high capacity and a good flow property
Average
Part No.
The type of resin
particle
Capacity
Application
size
Sepcificat
ion
To remove substances such as
IC-RP
divinylbenzene resin
40μm
300mg/1cc
cartridge
aromatic dyes, some aromatic
carboxylic acids, hydrocarbons,
10 pk
and surfactants from sample
matrices
To remove the phenolic fraction
IC-P
polyvinylpyrrolidone(PVP)
polymer resin
40μm
350mg/1cc
of humic acids, tannic acids,
cartridge
lignins, anthocyanins, and
10 pk
azodyes from samples.
16%
IC-A
cross-linked,styrene-based,anio
n-exchangeresin in
40μm
0.7meq/1cc
cartridge
thebicarbonate form.
10 pk
sample solution.
alkaline earths and transition
acid ,sulfonic
acid ,styrene-based,sulfonic
and neutralize the strongly acidic
To remove high levels of
16% cross-linked,sulfonic
IC-H
To remove anion contaminant
40μm
acid ,cation-exchangeresin in
2.0-2.2meq/1cc
metals from sample matrices and
cartridge
in the neutralization of highly
10 pk
alkaline samples such as sodium
thehydrogen form
hydroxide or sodium carbonate.
To remove high levels of
alkaline earths and transition
16% cross-linked,sulfonic
acid ,sulfonic
IC-Na
acid ,styrene-based,sulfonic
thesodium form.
40μm
2.0-2.2me/1cc
cartridge
metals from sample matrices
without acidifying the sample.
This ensures good recovery of
acid labile analytes such as
nitrite.
10 pk
To remove chloride, bromide,
16%
and iodide from sample
cross-linked,styrene-based,sulf
IC-Ag
onic acid,cation-exchangeresin
in the
40μm
2.0-2.2meq/1cc
matrices. An IC- H cartridge
cartridge
should be used after the IC- Ag
silverstyrene-based,styrene-bas
cartridge to remove dissolved
ed,form.
Ag.
10 pk
To remove SO42- For
reproducible, quantitative
determinations in
styrene-based,sulfonic
IC-Ba
40μm
thebarium form.
2.0-2.2meq/1cc
cartridge
low-ionic-strength samples,
these cartridges should be
10 pk
activated with a chloride-based
activating solution. Then the
added chloride need to be
removed.
IC-M
iminodiacetateresin in
theammonium form
40μm
0.4meqmg/1cc
cartridge
To remove transition metals and
matrix elimination of alkali and
10 pk
alkaline earth metals.
IC-Ag/H
It is a layered cartridge that contains IC- Ag resin and IC- H resin.
10 pk
IC-Ba/Ag/H
It is a layered cartridge that contains IC-Ba resin, IC- Ag resin and IC- H resin.
10 pk
The Development of SPE Method
There are many factors influenced the establishment of SPE, and the following are the four main ones which
must be considered:
1) The Selection of Sorbent Retention Mechanism
The guide on this page briefly outlines the decision making process required to choose a suitable extraction
mechanism.
2) The Selection of SPE Columns
The guide on this page briefly outlines the decision making process required to choose a suitable sorbent.
The selection of column packaging and the parameter of sample loading and elution
For the normal phase and reversed phase SPE cartridges, the weight of sample can not be exceed the 5%
of cartridges. For the ion-exchange mode, the capacity of the ion-exchange must be considered.
The table below is the capability and eluted parameter of SPE:
Specification
Quality of Loading Sample
The Minimum Volume of Elution
50mg/1mL
2.5mg
125μL
100mg/1mL
5mg
250μL
200mg/3mL
10mg
500μL
500mg/6mL
25mg
1.2mL
1g/6mL
50mg
2.4mL
3) The Selection of Ideal Elution Solvent
Applications
Determination of Oleic acid and its Metabolites in Human Plasm
SPE Columns
Cleanert PAX, 30mg/1mL
SPE Procedure
1) Condition: 1mL methanol
2) Equilibration: 1mL water
3) Sample loading: 500 μL standard sample(8% NH3•H2O)
4) Washing: 1 mL 8% NH3•H2O
5) Elution: 5% Formic acid in methanol
HPLC Conditions
Mobile phase: ACN: 3mmol/L ammonium acetate=85：15
HPLC column: Venusil ASB C18 Column（2.1×50mm）
MS Conditions
z
MS: Qtrap 3200
z
Ion Source: ESI
z
Scan Mode: Negative mode
z
Single ions monitor (SIM): Oleic acid m/z 281.2; Internal standard substances C17 m/z 269.2
z
Multiple reactive monitor (MRM): Metabolite of oleic acid m/z 315.2 →141.1
Determination of Pseudoephedrine
Hydrochloride and its Metabolites in Plasm
SPE Columns
Cleanert PCX, 100mg/1mL
SPE Procedure
1) Condition: 1mL methanol
3) Sample loading: 500 μL standard sample(8% NH3•H2O)
4) Washing: 1 mL 8% NH3•H2O
5) Elution: 5% Formic acid in methanol
HPLC Conditions
Mobile phase: A:1% Formic acid;B: Methanol
Venusil ASB C18 Column （2.1×50mm）
Flow rate: 0.5 mL/min
MS Conditions
Table of Gradient Elution Program
Time(min)
Mobile phase A
Mobile phase B
0.00
80
20
2.00
5
95
3.00
5
95
3.50
80
20
z
MS: Qtrap 3200
z
Ion Source: ESI
z
Scan Mode: Positive mode
z
Multiple reactive monitor (MRM): Pseudoephedrine Hydrochloride m/z 166.0 →148.1
z
Internal standard substances: Lidocaine m/z 235.3→86.1
Determination of Evodiamine and Rutecarpine in Human Serum by LC-MS
Description
Sample: 50ng/mL in human serum (dilute two times by pure water)
SPE column: Cleanert C18, 200mg/3mL
SPE Procedure
1) Column activation: 2mL MeOH; 2mL H2O
2) Sample loading: 2mL (diluted) sample, at flow rate of 0.5mL/min
3) Washing: 1mL × 2H2O; drying using N2.
4) Elution: 1mL× 2 Ethyl acetate
5) Evaporation: evaporate solvent under N2 at 40℃
6) Reconstitution: with 1mL MeOH/water (85/15) and filtrated using 0.45μm membrane
LC-MS Conditions
Agilent 1100/Trap XCT
Column: Venusil XBP-C18, 4.6 × 150mm, 5μm
Mobile phase: methanol/ammonium formate = 85/15
Flow: 0.5mL/min; Temp: 25℃; Inj: 10μL; MS: ESI
The Recovery of Evodiamine and Rutecarpine in Human Serum
Analyte
Concentration (ng/mL)
Mean Recovery(%)
R.S.D.(%)
520
98.1
5.24
52
97.1
2.32
510
91.5
2.48
51
90.5
3.90
Evodiamine
Rutecarpine
Extraction and Analysis of Malachite Green in Salmon
SPE Columns
Cleanert Alumina B; Cleanert SCX
SPE Procedure
1) Extract the ground fish with ACN; evaporate ACN; dissolve the
residue in CH2Cl2.
2) Put Alumina B column on the top of SCX; wash both with 5mL ACN
3) Load the sample (2-3mL) into Alumina B
4) Wash both columns with 5mL ACN
5) Throw away Alumina B, elute the sample from SCX column using
4mL ACN/ammonium acetate(0.1M, pH 4.5)=1:1
HPLC Conditions
Column: Venusil XBP-C18, 4.6 × 150mm, 5μm
Mobile phase: ACN/ammonium acetate(0.1M, pH 4.5)=80:20;
Flow rate: 2mL/min; Temperature: 35℃; Detection: UV 588nm
Determination of Clenbuterol in Urine Samples by Mixed- phase SPE and LC-MS
SPE Column
Cleanert C18/SCX (500 mg/3 mL, prototype)
SPE Method
1) Activate the column with 2 mL MeOH, 2 mL 10 mmol HCl aqueous solution;
2) Load 5 mL sample at the flow rate 1 mL/min
3) Rinse the column with 5 mL water, 5 mL MeOH, dry the column
4) Elute analyte with 5 mL 4% ammonia in MeOH.
5) Evaporate the solvent and reconstitute the sample in 1 mL 1% formic acid/MeOH = 9 : 1
Result
Recovery = 80-90% for 2 μg/kg dose, RSD<20%
The Analysis of Trace Chloramphenicol
SPE Column
Cleanert Florisil 1000mg/6mL strong cations.
SPE Method
1) Condition: 5ml methanol, 5ml ether.
2) Sample loading: 5mL sample
3) Wash: 5ml ether
4) Elution: methanol/ether=7/3
LC-MS Detection
Detection of Seven Hydroxybenzenes in Water (PEP)
Materials
1) SPE column: Cleanert PEP, 4-nitrophenol
2) Seven kinds of hydroxybenzene: phenol, Cresol, 4-nitrophenol, 2-Chlorophenol, 2,4-Dichlorophenol,
2,4,6-Trichlorophenol, Pentachloro phenol
SPE Procedure
1) Column Condition: 5mL Methyl tert-butyl ether/methanol(V/V=10:90) , 5mL/min;
3) Sample loading: 1L sample, 10mL/min
4) Washing: 10mL water, then drying using N2 for 20mins
5) Elution: 2mL Methyl tert-butyl ether
HPLC Conditions
Mobile phase: A: 1% acetic acid, B: 1% acetic acid and methanol
HPLC column: AQ C18 (4.6 × 150mm, 5μm); UV Detector
Time
Mobile Phase
Flow Rate (mL/min)
UV (nm)
0-15min
A:B=50:50
1
275
15-30min
A:B=15:85
1.8
295
The Extraction of Five Kinds of Sulfonamides in Pork
SPE Method
1) Condition: 5ml 95% acetonitrile
2) Load: 3mL sample which is dissolved by 95% acetonitrile
3) Wash: 5mL 95% acetonitrile
4) Elute:10mL 70% acetonitrile
Result of Recoveries of Five Kinds of Sulfonamides in Pork
Cleanert PEP Application Testing Examples
Nitroanilines
Sample
2-nitroaniline and 4-nitroaniline in 0.1% ammonia aqueous solution.
SPE Procedure
1) Activation, 3mL methanol, 3mL DI water
2) Loading, 3mL sample
3) Washing: 1mL 0.1% ammonia aqueous solution
4) Elution: 3mL methanol/1%formic acid = 95/5.
Drying with N2 and reconstitute the residue using 1mL methanol.
HPLC conditions
Venusil AQ C18 HPLC column, 4.6x250mm; Mobile phase: Methanol/1%formic acid = 10/90
Results
Sample
Peak Area(avg)
Concentr. (ppm)
Peak Area(avg) Std
a
20
296740.5
270652
91
b
2
28374.5
31517
111
c
0.2
2830.5
2947
104
a
20
464657.5
456445
98
b
2
45113.5
46537
103
c
0.2
3489.5
4234
121
4-nitroaniline
PEP
Recovery (%) PEP
Aniline and N, N-dimethylaniline
Sample
Aniline and N, N-dimethylaniline in DI water solution.
SPE Procedure
1) Activation, 3mL methanol, 3mL DI water
2) Loading, 3mL sample
3) Washing: 1mL water
4) Elution: 3mL methanol/water = 95/5
Drying with N2 and reconstitute the sample using 1mL methanol.
HPLC conditions
Venusil AQ-C18 column, 4.6 × 250mm, 5μm Mobile phase: methanol/water = 50/50
Results
Peak Area(avg)
Recovery (%)
PEP
PEP
360251
213975
60
20
36686
20672
56
c
2
3563
2870
81
a
200
1418380
1398427
98
b
20
174334
159897
92
c
2
19483
18386
94
d
0.2
2032
1841
91
Sample
Concentr. (ppm)
Peak Area(avg) Std
a
200
b
4-nitroaniline
Praziquantel
SPE Procedure
1) Condition and equilibrate: 2mL methanol, 2mL water
2) Load: 4mL sample(diluted by 0.2% acetic acid)
3) Wash: 1mL water
4) Elute: 4mL methanol
HPLC conditions
methanol: water=70 : 30, UV=263nm, H PLC column: Venusil AQ C18, 4.6 × 150mm, 5μm
Praziquantel st
Gliclazide
SPE Procedure
1) Condition and equilibrate: 1mL dichloromethane, 1mL water
2) Load: 4mL sample (diluted by 5mL 50% ACN and water and 10mM K2HPO4)
3) Elute: 4mL methanol
HPLC conditions
Promosil C18, 20μL, ACN: H2O=60/40, UV=235nm
Cross-reference Table
Agela
Waters
Supelco
Aglient
Varian
Cleanert C18
Sep-pak C18
ENVI-18 LC-C18
—
Bond Elut C18
Cleanert C18-N
—
—
AccuBOND C18
C8
Cleanert C8
Sep-pak C8
ENVI-8
AccuBOND C8
Bond Elut C8
CN Cyanopropyl
Cleanert CN
Sep-pak CN
LC-CN
AccuBOND CN
Bond Elut CN
NH2 Aminopropyl
Cleanert NH2
Sep-pak NH2
LC-NH2
AccuBOND NH2
Bond Elut NH2
Cleanert PSA
—
—
—
Bond Elut PSA
Cleanert SAX
—
LC-SAX
Cleanert COOH
—
LC-WCX
—
Cleanert PRS
—
—
—
Cleanert SCX
—
LC-SCX
Silica
Cleanert Silica
Sep-pak Silica
LC-Silica
Diol
Cleanert Diol
Sep-pak Diol
LC-Diol
PS
Cleanert PS
—
ENVI-Chrom P
PEP(Polar polymers)
Cleanert PEP
Oasis HLB
—
—
PAX
Cleanert PAX
Oasis MAX
—
—
—
PCX
Cleanert PCX
Oasis MCX
—
—
—
HXN
Cleanert HXN
—
—
—
—
ODS C18
ODS
C18-N(Non-endcapped)
PSA(N-aminoethyl
Aminopropyl)
SAX(Strong anion
exchanger)
COOH(Weak cation
exchanger)
PRS(Propane sulfonic acid)
SCX(Strong cation
exchanger)
AccuBOND
SAX
AccuBOND
SCX
Bond Elut
C18-OH
Bond Elut SAX
Bond Elut
CBA
Bond Elut PRS
Bond Elut SCX
AccuBOND
Bond Elut
Silica
Silica
AccuBOND Diol
Bond Elut Diol
AccuBOND
ENV PS-DVB
—
Bond Elut
Plexa
Purification Products
z
z
z
Preparative HPLC Columns -----------------------------------------------------Features -------------------------------------------------------------------------Ordering Information -------------------------------------------------------------Flash Chromatography Media and Columns ----------------------------------Flash Chromatography System --------------------------------------------------
116
118
120
120
125
Preparative HPLC Columns
Features
Agela Technologies offers a full line of preparative HPLC columns to meet a variety of application needs.
Our preparative columns feature:
1.Great scalability
2.Excellent bed stability
3.High loading capacity
4.Broad solvent compatibility,from 100% aqueous to 100% organic solvents (Unisol C18, Unisol Amide, and
Venusil ASB-C18)
5.Broad pH range,1-12 (Venusil Durashell)
6.Unique selectivity (Unisol Amide and Venusil ASB-C18)
Unisol-Amide: an unique phase from Agela Technologies that offers a good alternative selectivity to
reverse phase columns and an excellent solution for purifying highly polar compounds.
Durashell RP: High pH tolerance enables the use of high pH mobile phase, which results in great
improvement of peak shape and loadability of basic compounds.
Column：
Unisol Amiide , 4.6 x 250 mm, 5μm
Mobile phase：ACN / Water = 85/15~40/60 in 30 min
Detection：
UV 210nm
Excellent Bed Stability
Venusil XBP-C18, 20 x 150 mm, 10 μm，>1000 injection
Mass Load Comparison
Column:
C18, 20 x 150 mm, 5μm
Sample:
Diphenhydramine, 100 mg/mL in DMSO
Mobile phase: 10 mM ammonia: ACN=65: 35
Injection:
800μL
Analytical/Preparative HPLC Comparison
Analytical
Preparative
Purpose
Purpose
Information about the composition
Obtain pure components
Interested in most or all components
Interested in one or a few components
Characteristics
Characteristics
Small injection
Large injection
Reverse phase preferred
Normal phase may be preferred
1~5mm internal diameter
1~10cm internal diameter
2-5μm packing materials
>5μm packing materials
HPLC pump <10mL/min
HPLC pump ≥10mL/min
Injection usually trouble free
May have difficulties for sample injection
Maximize detection sensitivity
Minimize detection sensitivity
Sample solubility less important
Sample solubility very important
Mobile phase volatility very important; no-volatile additiveis
Mobile phase volatility less important
prohibited.
Pore Size: 100Å
Type
Dimension
Particle
Venusil
Venusil
Venusil
Durashell
Venusil
Venusil
(mm)
(μm)
XBP-C18
XBP-C8
Silica
RP
ASB-C18
ASB-C8
Semi-preparative
10×150
5
VX951510-0
VX851510-0
VSi951510-0
DS951510-0
VS951510-0
VS851510-0
Semi-preparative
10×250
5
VX952510-0
VX852510-0
VSi952510-0
DS952510-0
VS952510-0
VS852510-0
Preparative
20×50
5
VX950520-0
VX850520-0
VSi950520-0
DS950520-0
VS950520-0
VS850520-0
Preparative
20×150
5
VX951520-0
VX851520-0
VSi951520-0
DS951520-0
VS951520-0
VS851520-0
Preparative
20×250
5
VX952520-0
VX852520-0
VSi952520-0
DS952520-0
VS952520-0
VS852520-0
Preparative
30×100
5
VX951030-0
VX851030-0
VSi951030-0
DS951030-0
VS951030-0
VS851030-0
Preparative
30×150
5
VX951530-0
VX851530-0
VSi951530-0
DS951530-0
VS951530-0
VS851530-0
Preparative
30×250
5
VX952530-0
VX852530-0
VSi952530-0
DS952530-0
VS952530-0
VS852530-0
Guard cartridge
20×10
5
VX950120-0
VX850120-0
VSi950120-0
DS950120-0
VS950120-0
VS850120-0
Semi-preparative
10×150
10
VX901510-0
VX801510-0
VSi901510-0
DS901510-0
VS901510-0
VS801510-0
Semi-preparative
10×250
10
VX902510-0
VX802510-0
VSi902510-0
DS902510-0
VS902510-0
VS802510-0
Preparative
20×50
10
VX900520-0
VX800520-0
VSi900520-0
DS900520-0
VS900520-0
VS800520-0
Preparative
20×150
10
VX901520-0
VX801520-0
VSi901520-0
DS901520-0
VS901520-0
VS801520-0
Preparative
20×250
10
VX902520-0
VX802520-0
VSi902520-0
DS902520-0
VS902520-0
VS802520-0
Preparative
30×100
10
VX901030-0
VX801030-0
VSi901030-0
DS901030-0
VS901030-0
VS801030-0
Preparative
30×150
10
VX901530-0
VX801530-0
VSi901530-0
DS901530-0
VS901530-0
VS801530-0
Preparative
30×250
10
VX902530-0
VX802530-0
VSi902530-0
DS902530-0
VS902530-0
VS802530-0
Preparative
50×150
10
VX901550-0
VX801550-0
VSi901550-0
DS901550-0
VS901550-0
VS801550-0
Preparative
50×250
10
VX902550-0
VX802550-0
VSi902550-0
DS902550-0
VS902550-0
VS802550-0
Guard cartridge
20×10
10
VX900120-0
VX800120-0
VSi900120-0
DS900120-0
VS900120-0
VS800120-0
Pore Size: 100Å
Dimension
Particle
(mm)
(μm)
Semi-preparative
10×150
5
UO951510-0
UA951510-0
Semi-preparative
10×250
5
UO952510-0
UA952510-0
Preparative
20×50
5
UO950520-0
UA950520-0
Preparative
20×150
5
UO951520-0
UA951520-0
Preparative
20×250
5
UO952520-0
UA952520-0
Preparative
30×100
5
UO951030-0
UA951030-0
Preparative
30×150
5
UO951530-0
UA951530-0
Preparative
30×250
5
UO952530-0
UA952530-0
Guard cartridge
20×10
5
UO950120-0
UA950120-0
Semi-preparative
10×150
10
UO901510-0
UA901510-0
Semi-preparative
10×250
10
UO902510-0
UA902510-0
Preparative
20×50
10
UO900520-0
UA900520-0
Preparative
20×150
10
UO901520-0
UA901520-0
Preparative
20×250
10
UO902520-0
UA902520-0
Preparative
30×100
10
UO901030-0
UA901030-0
Preparative
30×150
10
UO901530-0
UA901530-0
Preparative
30×250
10
UO902530-0
UA902530-0
Preparative
50×150
10
UO901550-0
UA901550-0
Preparative
50×250
10
UO902550-0
UA902550-0
Guard cartridge
20×10
10
UO900120-0
UA900120-0
Type
Unisol C18
Unisol
Amide
Flash Chromatography Media and Columns
Column chromatography has been and will be continuously used as a major purification method for
synthetic and natural products. Traditional silica-based flash chromatography columns have many
drawbacks: 1) high surface activity which causes instability of certain compounds, 2) bad tailing or
overly-long retention of basic compounds due to acid/base interaction or metal chelating effect, and 3) poor
reproducibility.
Agela Technologies Inc developed a technology that can specially deactivate the silica media. Our columns
offer significantly improved performance and much less undesirable silica surface activities than the regular
flash columns. Our columns may also provide alternative selectivity.
Flash CS Silica Columns
Ultra pure silica (specially washed with acid and DI water, narrow particle size control and water content
control)
Column Specification
Surface Area: 460- 520m2/g ;Average Pore Size: 52- 68Å ;Surface pH: 6.3-7.2;Water content:
3.0-5.0% ;Average Particle Size: 40-60μm
Column
Flash Column Size (g)
Efficiency (phenylacetone)
120
2000
176
80
1800
108
40
1100
54.6
20
900
30.5
12
700
17.9
4
500
8
Vol.(mL)
Aniline Flash Column Tests Report
Column: Agela Cleanert CS (40 g), Brand I (40 g Si)
Mobile phase: Dichloromethane : methanol = 99 : 1
Detection: 254 nm
Flow rate: 20 mL/min
Rt: Brand I: 4.373 min; Agela: 4.090 min
Chromatography Comparison
Sample: Phenyl acetone , 4-aminobenzoicacid
Mobile phase: Hexane/ethylacetate (gradient)
Detection: 254 nm
Flash CM Silica Columns
The following figures present a comparison of column performance for an Agela’s deactivated silica column
vs an unmodified silica flash column.
Chromatogram Comparison of Catechol on Different Silica
Test:
The silica to be tested was packed into a stainless steel
column of 4.6 × 150mm and tested on a HPLC system
Conditions:
Sample: Catechol 100μg/mL in mobile phase
Mobile phase: dichloromethane/methanol = 98/2
Temperature: 30℃
Detection: 254nm
Injection: 5μL
Silica Characteristics:
Average Particle Size: 50μm
Average Pore Size: 60Å
Shape: Irregular
Surface Area: 500m2/g
HILIC Flash Chromatography
All HILIC phases are available for TLC, HPLC and Flash columns
Following the success of Agela’s Venusil HILIC HPLC columns, we are now introducing HILIC phases for
the Flash Chromatography applications. Agela’s HILIC columns are compatible with polar and aqueous
solvents, such methanol, acetonitrile, and water, which makes the solvent handling much easier compared
to that for the normal phase separations. Significantly, because of the easy operation principle in using
Agela’s HILIC columns, these columns are designed to last. You will get a great return of the column
investment at an already truly affordable price.
Applications for HILIC columns are listed below:
1. Solution for compounds that re not soluble in non- or low polar solvents (hexane, IPA, toluene,
dichloromethane).
2. Solution for highly polar compounds which have too much retention on silica columns.
3. Solution for a mixture of compounds with a broad range of polarity (non-polar, semi-polar, and high polar)
with alternative selectivity.
More important, you will get all the benefits at a truly affordable price, and may even lower the cost by
multiple uses of the columns!
Column:
20g HILIC column
Sample:
10 mg of uridine in 3 mL hot water
Mobile phase: water
Sample:
Detection:
254 nm
Flow rate:
12 mL/min
100mg VC and 70mg VB2, direct solid loading
Sample:
100mg VC and 70mg VB2, direct solid loading
Mobile phase: 0-2min EtOAc; 2-22min MeOH(0-100%); 22-52min MeOH
Mobile phase: 0.1% TFA : ACN = 99 : 1
Detection:
280 nm
Detection:
280 nm
Flow rate:
25 mL/min
Flow rate:
25 mL/min
Quick Work-up Cartridge
They are specially designed to replace the conventional aqueous work up /liquid-liquid extraction process as
a part of organic synthesis and purification.They are the through-columns packed with wet resin and/or
diatomite.
How to use :
1 a synthetic mixture is injected directly injected onto a column.
2 chose a solvent which is not immiscible with water to rinse the desired compounds out of column.
3 concentrate the collected solution
4 add the concentrated mixture to a flash column or a HPLC column for further separation.
Phase
Celite
C18 (RP)
HILIC
SCX (retaining amines)
SAX (retaining acids)
PEP (RP)
Catalyst(metal) remover
Size
Type
Cat. No.
1mL
I
QWC001
3mL
II
QWC003
6mL
II
QWC006
1mL
I
QW9001
3mL
II
QW9003
6mL
II
QW9006
1mL
I
QWH001
3mL
II
QWH003
6mL
II
QWH006
1mL
I
QWS001
3mL
II
QWS003
6mL
II
QWS006
1mL
I
QWA001
3mL
II
QWA003
6mL
II
QWA006
1mL
I
QWP001
3mL
II
QWP003
6mL
II
QWP006
1mL
I
QWM001
3mL
II
QWM003
6mL
II
QWM006
(Compatible with ISCO, Buchi and Biotage flash chromatography instruments)
Surface Type
Silica (CS)
Silica (CM)
Silica NH2
Silica ODS
HILIC
Silica Amount (g)
Cat. Number
Quantity (pk)
4
CS140004-0
20
12
CS140012-0
20
20
CS140020-0
20
40
CS140040-0
10
80
CS140080-0
5
120
CS140120-0
5
330
CS140330-0
1
800
CS140800-0
1
1500
CS141500-0
1
2500
CS142500-0
1
4
CM140004-0
20
12
CM140012-0
20
20
CM140020-0
20
40
CM140040-0
10
80
CM140080-0
5
120
CM140120-0
5
330
CM140330-0
1
800
CM140800-0
1
4
CN140004-0
20
12
CN 140012-0
20
20
CN 140020-0
20
40
CN 140040-0
10
80
CN 140080-0
5
120
CN 140120-0
5
330
CN 140330-0
1
800
CN 140800-0
1
4
CO140004-0
20
12
CO140012-0
20
20
CO140020-0
20
40
CO140040-0
10
80
CO140080-0
5
120
CO140120-0
5
330
CO140330-0
1
800
CO140800-0
1
4
CH140004-0
20
12
CH140012-0
20
20
CH140020-0
10
40
CH140040-0
10
80
CH140080-0
5
120
CH140120-0
5
330
CH140330-0
1
800
CS140800-0
1
CS: regular silica; CM: general deactivated, low metal and less retentive; NH2: aminopropyl; ODS: C18 reverse phase;
HILIC: Hydrophilic interaction column.
Bulk Silica
Cat. No.
Grade
Particle Size
Pack
CS605005-P
P
40-60μm
5kg
CS605025-P
P
40-60μm
25kg
CS605005-G
G
40-60μm
5kg
CS605025-G
G
40-60μm
25kg
CS605001-P
P
40-60μm
1kg
CS605002-P
P
40-60μm
2.5kg
CS605001-G
G
40-60μm
1kg
CS605002-G
G
40-60μm
2.5kg
CS608005-P
P
70-90μm
5kg
CS608025-P
P
70-90μm
25kg
CS608005-G
G
70-90μm
5kg
CS608025-G
G
70-90μm
25kg
CS608001-P
P
70-90μm
1kg
CS608002-P
P
70-90μm
2.5kg
CS608001-G
G
70-90μm
1kg
CS608002-G
G
70-90μm
2.5kg
P: Reagent Grade; G: Tech.Grade
Fritted Empty Cartridges for Filtration or Solid Sample Loading with Screw Caps
Volume (mL)
Cat. No.
Quantity (pk)
3
SLC 0003
50
6
SLC 0006
50
10
SLC 0008
50
30
SLC 0024
30
60
SLC 0040
20
120
SLC 0120
20
Volume (mL)
Cat. No.
Quantity (pk)
10 mL
SLS 0008
1
30 mL
SLS 0024
1
50 mL
SLS 0040
1
Piston Seals, Cartridge
Flash Chromatography System
Flash chromatography, is a rapid form of preparative column chromatography, is especially useful in
separation and purification of large scale of complex organic intermixture, combinatorial chemistry, drug
discovery and natural product research and development.
Minimize Method Development Time and Maximize Results!
complete most purification within 20 minutes
Features & Capabilities
Agela FS-2100 "Chemistry-centric" design for all purpose liquid chromatography applications
Windows interface wizard method development software with touch screen makes purification a snap, we can adjust the
distance between the touch screen and the manager
Compact Profile design for limited fume hood space
Column accepts any compression module/cartridge combination
Pump Patented valveless metering pump, maintenance free, low pulse. Suitable for all organic solvent, good resistance
for acid and alkali.
Automatic pressure monitoring & alarm for over/under-pressure conditions
Exposed accessible fluid path for no leaky surprises. Easily maintain all components
Detector adjustable wavelength 190 to 600nm
Fraction High volume fraction collection to correlate fractions with chromatogram display
Split fraction rack design allows pause-free extended runs and offline tube changes
Peak software generates an optimized purification run. Flow rates are automatically maximized whenever possible to
speed purification.
Optimized conditioning with pause for sample injection
Modify the method Pause and resume the run as necessary.
Collect mode Peak, volume, windows
Agela FS-2100
Flash Chromatography Workstation
Specification
Feature/Specification
Description
Purification Channels
1
Column
Cartridge Sizes
4-330g
System
Solid Sample Capacity
5mg-33g using prepacked SPE-type barrels or self-loaded cartridges
Liquid Sample Injection
5-35mL, luer taper syringe port
Solvent Delivery
Patented valveless metering pump
Flow rate
5-100 mL/min. (±1% accuracy), 50-500 mL/min optional
Gradient (# of solvents)
Binary (2) standard (one optional)
System Max Pressure
100 psi (7bar) included software low / high cut-off alarm
UV Detection
Field changeable filters for 190-600nm wavelength
Fraction Tube Sizes
13, 15, 18, 24mm tube racks, scintillation vials, funnels
Rack Design
Two rack design for extended run without pause and offline tube changes
Fraction
Architecture
Touch Screen
Collector
Input
Touch Screen
Video
10.4" TFT, solvent resistant
Ports
USB,RS-485
Chromatogram display
Real-time color , with historical pan/zoom/scroll by time & AU
Off-line Run Review
Open saved run information for off-line analysis on the touch screen
Auto Configuration
"Wizard" guided setup with manual override for advanced users
Sample ID
Sample ID identifies samples in saved run data
File system
Standard Windows with extended file and folder names
Power
100-240V 50/60 Hz
Dimensions HxWxD (cm)
60 x 48 x 48cm( 24" x 19" x 19")
Warranty
One year limited warranty (ask sales representative for details)
Flow Pump
UV Detector
Peak Software
General
GC Columns
z
z
z
z
z
z
z
z
z
z
z
z
z
z
z
z
z
z
z
z
DA-1 -----------------------------------------------------------------------------DA-1MS -------------------------------------------------------------------------DA-5 -----------------------------------------------------------------------------DA-5MS -------------------------------------------------------------------------DA-35MS ------------------------------------------------------------------------DA-1701 ------------------------------------------------------------------------DA-CarBoWax 20M ------------------------------------------------------------DA-FFAP ------------------------------------------------------------------------DA-INOWAX --------------------------------------------------------------------DA-50+MS ----------------------------------------------------------------------DA-1301 -------------------------------------------------------------------------DA-624 ---------------------------------------------------------------------------DA-PLOT Al 2 O 3 “KCl” -----------------------------------------------------------DA-PLOT Al 2 O 3 “S” -------------------------------------------------------------DA-PLOT Al 2 O 3 “M” ------------------------------------------------------------DA-PLOT MoleSieve -----------------------------------------------------------DA-PLOT GasPro ---------------------------------------------------------------DA-PLOT Q ----------------------------------------------------------------------DA-PLOT U ----------------------------------------------------------------------DA-Blood Alcohol Column ------------------------------------------------------
128
129
130
131
132
132
133
133
134
135
136
137
138
139
140
141
142
143
144
144
DA-1
z
100% bonded and crosslinked dimethylpolysiloxane
z
Non-polar, equivalent to USP phase G2
z
Solvent rinseable and high temperature limit
z
Wide range of column dimensions and stationary phase film thicknesses
z
Low column bleed, inert surface and high efficiency
z
Most polular for general applications
Similar Phases: DB-1, HP-1, Ultra-1, SPB-1, RTX-1, CP-Si 5CB, BP-1, ZB-1, AT-1, MDN-1, OV-1, OV101
ID(mm)
Length(m)
Film(μ
Temperature
m)
Limit(℃)
Cat.No.
0.25
15
0.1
-60 to 325/350
0125-1501
0.25
15
0.25
-60 to 325/350
0125-1502
0.25
15
1
-60 to 325/350
0125-1510
0.25
30
0.1
-60 to 325/350
0125-3001
0.25
30
0.25
-60 to 325/350
0125-3002
0.25
30
1
-60 to 325/350
0125-3010
0.25
60
0.25
-60 to 325/350
0125-6002
0.25
60
1
-60 to 325/350
0125-6010
0.25
100
0.5
-60 to 325/350
0125-0010
0.32
15
0.25
-60 to 325/350
0132-1502
0.32
15
1
-60 to 325/350
0132-1510
0.32
25
0.17
-60 to 325/350
0132-2501
0.32
25
0.52
-60 to 325/350
0132-2505
0.32
25
1.05
-60 to 325/350
0132-2510
0.32
30
0.1
-60 to 325/350
0132-3001
0.32
30
0.25
-60 to 325/350
0132-3002
0.32
30
1
-60 to 325/350
0132-3010
0.32
30
3
-60 to 325/280
0132-3030
0.32
30
4
-60 to 325/280
0132-3040
0.32
30
5
-60 to 325/280
0132-3050
0.32
50
0.17
-60 to 325/350
0132-5001
0.32
50
0.52
-60 to 325/350
0132-5005
0.32
50
1.05
-60 to 300/320
0132-5010
0.32
60
0.25
-60 to 325/350
0132-6002
0.32
60
1
-60 to 300/320
0132-6010
0.32
60
5
-60 to 260/280
0132-6050
0.53
5
0.15
-60 to 325/360
0153-0501
0.53
5
0.88
-60 to 325/360
0153-0508
0.53
5
2.65
-60 to 260/280
0153-0526
0.53
7.5
5
-60 to 260/280
0153-0750
0.53
10
0.88
-60 to 300/320
0153-1008
0.53
10
2.65
-60 to 260/280
0153-1026
0.53
15
0.15
-60 to 320/360
0153-1501
0.53
15
1.5
-60 to 300/320
0153-1515
0.53
15
3
-60 to 260/280
0153-1530
0.53
15
5
-60 to 260/280
0153-1550
0.53
30
0.88
-60 to 300/320
0153-3008
0.53
30
1.5
-60 to 300/320
0153-3015
0.53
30
2.65
-60 to 260/280
0153-3026
0.53
30
3
-60 to 260/280
0153-3030
0.53
30
5
-60 to 260/280
0153-3050
0.53
60
5
-60 to 260/280
0153-6050
DA-1MS
z
Identical selectivity to DA-1,but with much lower column bleed
z
Ideal for GC-MS analysis and first choice of new method development
Similar phases: HP 1MS,DB 1MS,BPX-1,AT 1MS,ZB-1,Rtx-1,CP-Sil 5CB
Temperature
ID(mm)
Length(m)
Film(μm)
0.25
15
0.25
-60 to 325/350
1125-1502
0.25
30
0.1
-60 to 325/350
1125-3001
0.25
30
0.25
-60 to 325/350
1125-3002
0.25
30
0.5
-60 to 325/350
1125-3005
0.25
30
1
-60 to 325/350
1125-3010
0.25
60
0.25
-60 to 325/350
1125-6002
0.32
15
0.25
-60 to 325/350
1132-1502
0.32
25
0.52
-60 to 325/350
1132-2505
0.32
30
0.1
-60 to 325/350
1132-3001
0.32
30
0.25
-60 to 325/350
1132-3002
0.32
30
1
-60 to 325/350
1132-3010
0.32
60
0.25
-60 to 325/350
1132-6002
Limit(℃)
Cat.No.
DA-5
z
5% phenyl, 95% methylpolysiloxane, bonded and crosslinked
z
Non-polar, equivalent to USP phase G27
z
Solvent rinseable and high temperature limit
z
Wide range of column dimensions and stationary phase film thicknesses
z
Low column bleed, inert surface with neutral surface and high efficiency
z
Most popular for general applications
Similar Phases: DB-5, HP-5, Ultra-5, SPB-5, RTX-5, CP-Sil 8CB, BP-5, ZB-5, AT-5, MDN-5, OV-5, SE-52,
SE-54
Temperature
Length(m)
Film(μm)
0.25
15
0.25
-60 to 325/350
0525-1502
0.25
15
1
-60 to 325/350
0525-1510
0.25
30
0.1
-60 to 325/350
0525-3001
0.25
30
0.25
-60 to 325/350
0525-3002
0.25
30
1
-60 to 325/350
0525-3010
0.25
60
0.25
-60 to 325/350
0525-6002
0.25
60
1
-60 to 325/350
0525-6010
0.32
15
0.25
-60 to 325/350
0532-1502
0.32
15
1
-60 to 325/350
0532-1510
0.32
25
0.17
-60 to 325/350
0532-2501
0.32
25
0.52
-60 to 325/350
0532-2505
0.32
25
1.05
-60 to 325/350
0532-2510
0.32
30
0.1
-60 to 325/350
0532-3001
0.32
30
0.25
-60 to 325/350
0532-3002
0.32
30
0.5
-60 to 325/350
0532-3005
0.32
30
1
-60 to 325/350
0532-3010
0.32
50
0.17
-60 to 325/350
0532-5001
0.32
50
0.52
-60 to 325/350
0532-5005
0.32
50
1.05
-60 to 325/350
0532-5010
0.32
60
0.25
-60 to 325/350
0532-6002
0.32
60
1
-60 to 260/280
0532-6010
0.53
10
2.65
-60 to 260/280
0553-1026
0.53
15
1.5
-60 to 300/320
0553-1515
0.53
15
5
-60 to 260/280
0553-1550
0.53
30
0.88
-60 to 300/320
0553-3008
0.53
30
1.5
-60 to 300/325
0553-3015
0.53
30
2.65
-60 to 260/280
0553-3026
0.53
30
5
-60 to 260/280
0553-3050
ID(mm)
Limit(℃)
Cat.No.
DA-5MS
z
Identical selectivity to DB-5, but with much lower column bleed
z
Best surface inertness for wide range of compounds
z
Ideal for GC-MS analysis or new method development
Similar Phases: HP-5MS, DB-5MS, BPX-5MS, AT-5MS, Rtx-5MS, CP-Sil 8 CB
Temperature
ID(mm)
Length(m)
Film(μm)
0.25
15
0.1
-60 to 325/350
1525-1501
0.25
15
0.25
-60 to 325/350
1525-1502
0.25
15
0.5
-60 to 325/350
1525-1505
0.25
15
1
-60 to 325/350
1525-1510
0.25
30
0.1
-60 to 325/350
1525-3001
0.25
30
0.25
-60 to 325/350
1525-3002
0.25
30
0.5
-60 to 325/350
1525-3005
0.25
30
1
-60 to 325/350
1525-3010
0.25
60
0.1
-60 to 325/350
1525-6001
0.25
60
0.25
-60 to 325/350
1525-6002
0.25
60
0.5
-60 to 325/350
1525-6005
0.25
60
1
-60 to 325/350
1525-6010
0.32
15
0.1
-60 to 325/350
1532-1501
0.32
15
0.25
-60 to 325/350
1532-1502
0.32
15
0.5
-60 to 325/350
1532-1505
0.32
15
1
-60 to 325/350
1532-1510
0.32
25
0.52
-60 to 325/350
1532-2505
0.32
30
0.1
-60 to 325/350
1532-3001
0.32
30
0.25
-60 to 325/350
1532-3002
0.32
30
0.5
-60 to 325/350
1532-3005
0.32
30
1
-60 to 325/350
1532-3010
0.32
60
0.1
-60 to 325/350
1532-6001
0.32
60
0.25
-60 to 325/350
1532-6002
0.32
60
0.5
-60 to 325/350
1532-6005
0.32
60
1
-60 to 325/350
1532-6010
Limit(℃)
Cat.No.
DA-35MS
z
Bonded and 35% phenyl backbone 65% dimethylsiloxane phase
z
Equivalent to USP phase G-42
z
Inert to many active compounds
z
Low column bleed to improve detection sensitivity and quantitation
z
Applications: Acroclors, PCBs, amines, pesticides, pharmaceutical molecules.
Similar Phases: HP-35MS, DB-35, BPX-35, AT-35, Rtx-35, SPB-35
Temperature
ID(mm)
Length(m)
Film(μm)
0.25
15
0.25
40 to 320/340
3525-1502
0.25
30
0.15
40 to 320/340
3525-3001
0.25
30
0.25
40 to 320/340
3525-3002
0.25
60
0.25
40 to 320/340
3525-6002
0.32
15
0.25
40 to 320/340
3532-1502
0.32
30
0.25
40 to 320/340
3532-3002
0.32
60
0.25
40 to 320/340
3532-6002
0.53
15
1
40 to 300/320
3553-1510
0.53
30
0.5
40 to 300/320
3553-3005
0.53
30
1
40 to 300/320
3553-3010
Limit(℃)
Cat.No.
DA-1701
z
Bonded and crosslingked 14% Cyanopropyl-phenyl 86% dimethylsiloxane phase
z
Low to mid polarity
z
z
Applications: pesticides, herbiciders, halogenates. Good confirmation of analysis of active compounds
on DA-5MS columns.
Similar phases: DB-1701, ZB-1701, BP-10, Rtx-1701, CP-Sil 19 CB
Temperature
ID(mm)
Length(m)
Film(μm)
0.25
15
0.15
-20 to 280/300
6125-1501
0.25
15
0.25
-20 to 280/300
6125-1502
0.25
15
1
-20 to 280/300
6125-1510
0.25
30
0.15
-20 to 280/300
6125-3001
0.25
30
0.25
-20 to 280/300
6125-3002
0.25
30
1
-20 to 280/300
6125-3010
0.25
60
0.15
-20 to 280/300
6125-6001
0.25
60
0.25
-20 to 280/300
6125-6002
0.25
60
1
-20 to 280/300
6125-6010
0.32
15
0.15
-20 to 280/300
6125-1501
Limit(℃)
Cat.No.
DA-CarBoWax 20M
z
Polyethylene glycol (PEG), MW 20,000
z
Non-Bonded non-crosslinked phase
z
Polar phase, equivalent to USP phase G16
z
Replacement of packed columns with Carbowax 20M modified
Temperature
ID(mm)
Length(m)
Film(μm)
0.2
25
0.1
60 to 220
2220-2501
0.2
50
0.1
60 to 220
2220-5001
0.25
30
0.25
60 to 220
2225-3002
0.32
25
0.3
60 to 220
2232-2503
0.32
50
0.3
60 to 220
2232-5003
0.53
10
1.33
60 to 210
2253-1013
0.53
30
1.33
60 to 210
2253-3013
Limit(℃)
Cat.No.
DA-FFAP
z
Nitroterephthalic acid modified Polyethylene glycol (PEG)
z
Bonded and crosslinked
z
Non-alcohol/aqueous solvent rinseable
z
High temperature limit
z
Polar phase equivalent to USP phase G35
z
Designed for the analysis of volatitle fatty acids and phenols
Simlar Phases: HP-FFAP, DB-FFAP, CB-WAX58 (FFAP) CB, Stabilwax-DA, BP-21, 007-CW, AT-100
Temperature
ID(mm)
Length(m)
Film(μm)
0.25
15
0.25
60 to 240/250
2125-1502
0.25
30
0.25
60 to 240/250
2125-3002
0.32
15
0.25
60 to 240/250
2132-1502
0.32
25
0.5
60 to 240/250
2132-2505
0.32
30
0.25
60 to 240/250
2132-3002
0.32
50
0.5
60 to 240/250
2132-5005
0.53
10
1
60 to 240
2153-1010
0.53
15
1
60 to 240
2153-1510
0.53
30
1
60 to 240
2153-3010
Limit(℃)
Cat.No.
DA-INOWAX
z
Bonded and crosslinked Polyethylene glycol (PEG)
z
Solvent washable
z
Highest temperature limits of bonded PEG phases
z
Polar phase, equivalent to USP phase G16
z
Wide applications of polar solvent analysis in food, pharma., cosmetics, petrochemical industries
Similar Phases: HP-INNOWax, DB-WAX, DB-WAXetr, SupelcoWAX 10, CP-WAX 52 CB, CB-WAX, Stablilwax, BP-20,
007-CW, AT-Wax, Rtx-Wax, ZB-Wax, HP-20M
ID(mm)
Length(m)
Film(μm)
Temperature
Limit(℃)
Cat.No.
0.2
25
0.2
40 to 260/280
2020-2502
0.2
25
0.4
40 to 260/280
2020-2504
0.2
50
0.2
40 to 260/280
2020-5002
0.2
50
0.4
40 to 260/280
2020-5004
0.25
15
0.25
40 to 260/280
2025-1502
0.25
15
0.5
40 to 260/280
2025-1505
0.25
30
0.15
40 to 260/280
2025-3001
0.25
30
0.25
40 to 260/280
2025-3002
0.25
30
0.5
40 to 260/280
2025-3005
0.25
60
0.15
40 to 260/280
2025-6001
0.25
60
0.25
40 to 260/280
2025-6002
0.25
60
0.5
40 to 260/280
2025-6005
0.32
15
0.25
40 to 260/280
2032-1502
0.32
15
0.5
40 to 260/280
2032-1505
0.32
30
0.15
40 to 260/280
2032-3001
0.32
30
0.25
40 to 260/280
2032-3002
0.32
30
0.5
40 to 260/280
2032-3005
0.32
60
0.15
40 to 260/280
2032-6001
0.32
60
0.25
40 to 260/280
2032-6002
0.32
60
0.5
40 to 260/280
2032-6005
0.53
15
1
40 to 240/260
2053-1510
0.53
30
1
40 to 240/260
2053-3010
0.53
60
1
40 to 240/260
2053-6010
DA-50+MS
z
Bonded and crosslinked 50% phenyl backbone 50% dimethylsiloxane phase
z
Equivalent to USP phase G-3
z
z
Low column bleed to improve detection sensitivity and quantization
z
Applications: Common drug screens, phenols, PAHs, free steroids, pesticides, herbicides. Good
confirmation of the analysis of active compounds on AB-5MS or AB-35MS columns
Similar Phases: HP-50+, DB -7, DB-17MS, BPX-50, AT-50, Rtx-50, SPB-50
Temperature
ID(mm)
Length(m)
Film(μm)
0.2
12
0.16
40 to 320/340
5020-1201
0.2
12
0.31
40 to 320/340
5020-1203
0.2
25
0.16
40 to 320/340
5020-2501
0.2
25
0.31
40 to 320/340
5020-2503
0.2
50
0.31
40 to 320/340
5020-5003
0.25
15
0.15
40 to 320/340
5025-1501
0.25
15
0.25
40 to 320/340
5025-1502
0.25
15
0.5
40 to 320/340
5025-1505
0.25
30
0.15
40 to 320/340
5025-3001
0.25
30
0.25
40 to 320/340
5025-3002
0.25
30
0.5
40 to 320/340
5025-3005
0.25
60
0.25
40 to 320/340
5025-6002
0.32
15
0.15
40 to 320/340
5032-1501
0.32
15
0.25
40 to 320/340
5032-1502
0.32
15
0.5
40 to 320/340
5032-1505
0.32
30
0.15
40 to 320/340
5032-3001
0.32
30
0.25
40 to 320/340
5032-3002
0.32
30
0.5
40 to 320/340
5032-3005
0.32
60
0.25
40 to 320/340
5032-6002
0.32
60
0.5
40 to 320/340
5032-6005
0.53
15
1
40 to 300/320
5053-1510
0.53
30
0.5
40 to 300/320
5053-3005
0.53
30
1
40 to 300/320
5053-3010
Limit(℃)
Cat.No.
DA-1301
z
Bonded and crosslinked 6% Cyanopropyl-phenyl 94% dimethylsiloxane phase
z
Equivalent to USP phase G-43, low to mid polarity
z
z
Applications: pesticides, herbiciders, halogenates. Good confirmation of the analysis of active compounds on
DA-5MS or DA-35MS columns
Similar Phases: HP-1301, DB-1301, BP-10, Rtx-1301
Temperature
ID(mm)
Length(m)
Film(μm)
0.25
15
0.25
-20 to 280/300
6025-1502
0.25
15
1
-20 to 280/300
6025-1510
0.25
30
0.25
-20 to 280/300
6025-3002
0.25
30
1
-20 to 280/300
6025-3010
0.25
60
0.25
-20 to 280/300
6025-6002
0.25
60
1
-20 to 280/300
6025-6010
0.32
15
0.25
-20 to 280/300
6032-1502
0.32
15
1
-20 to 280/300
6032-1510
0.32
30
0.25
-20 to 280/300
6032-3002
0.32
30
1
-20 to 280/300
6032-3010
0.32
60
0.25
-20 to 280/300
6032-6002
0.32
60
1
-20 to 280/300
6032-6010
0.53
15
1
-20 to 260/280
6053-1510
0.53
30
1
-20 to 260/280
6053-3010
0.53
30
1.5
-20 to 260/280
6053-3015
Limit(℃)
Cat.No.
DA-624
z
Bonded and crosslinked specially designed stationary phase for EPA methods
z
Mid-polarity
z
z
Applications: EPA methods: 501.3, 502.2, 503.1, 524.2, 601, 602, 8010, 8015, 8020, 8240,8260
Similar Phases: DB-624, ZB-624, AT-624, Rtx-624, CP-624
Temperature
ID(mm)
Length(m)
Film(μm)
0.2
25
1.12
-20 to 260
6220-2511
0.25
30
1.4
-20 to 260
6225-3014
0.25
60
1.4
-20 to 260
6225-6014
0.32
30
1.8
-20 to 260
6232-3018
0.32
60
1.8
-20 to 260
6232-6018
0.53
30
3
-20 to 260
6253-3030
0.53
60
3
-20 to 260
6253-6030
0.53
75
3
-20 to 260
6253-7530
Limit(℃)
Cat.No.
DA-PLOT Al2O3 “KCl”
z
Least selective ("polar") aluminum oxide phase
z
Deactivated with KCl
z
Good baseline separation of most C1 to C10 hydrocarbons
z
Olefins may co-elute with corresponding paraffin over C5
z
Minimum surface adsorption of dienes and halocarbons
z
ASTM recommended phase
z
Preferred choice of hydrocarbon separation
Similar Phases: HP-PLOT Al2O3/KCL, GS-Alumina/KCL, CP-Al2O3/KCl PLOT, ZB-Alumina/KCl
Temperature
ID(mm)
Length(m)
Film(μm)
0.25
25
5
-60 to 200/250
8125-2505
0.25
30
5
-60 to 200/250
8125-3005
0.32
15
8
-60 to 200/250
8132-1508
0.32
30
8
-60 to 200
8132-3008
0.32
50
8
-60 to 200
8132-5008
0.53
15
15
-60 to 200/250
8153-1515
0.53
30
15
-60 to 200
8153-3015
0.53
50
15
-60 to 200
8153-5015
0.53
60
15
-60 to 200
8153-6015
Limit(℃)
Cat.No.
DA-PLOT Al2O3 “S”
z
Good selective (mid "polar") aluminum oxide phase
z
Deactivated with Na2SO4 salt
z
Excellent baseline separation of C1 to C10 hydrocarbons, excellent of C3, C4 isomer baseline
separations
z
Less accurate quantitations of dienes and halocarbons
z
z
Similar Phases: HP-PLOT Al2O3/"S", GS-Alumina, CP-Al2O3/Na2SO4 PLOT, ZB-Alumina, RT-Alumina
Temperature
ID(mm)
Length(m)
Film(μm)
0.25
25
5
-60 to 200/250
8225-2505
0.25
30
5
-60 to 200/250
8225-3005
0.32
15
8
-60 to 200/250
8232-1508
0.32
30
8
-60 to 200
8232-3008
0.32
50
8
-60 to 200
8232-5008
0.53
15
15
-60 to 200/250
8253-1515
0.53
30
15
-60 to 200
8253-3015
0.53
50
15
-60 to 200
8253-5015
0.53
60
15
-60 to 200
8253-6015
0.25
25
5
-60 to 200/250
8325-2505
0.25
30
5
-60 to 200/250
8325-3005
0.32
15
8
-60 to 200/250
8332-1508
0.32
30
8
-60 to 200
8332-3008
0.32
50
8
-60 to 200
8332-5008
0.53
15
15
-60 to 200/250
8353-1515
0.53
30
15
-60 to 200
8353-3015
0.53
50
15
-60 to 200
8353-5015
0.53
60
15
-60 to 200
8353-6015
Limit(℃)
Cat.No.
DA-PLOT Al2O3 “M”
z
Strong selective (most "polar") aluminum oxide phase
z
Deactivated with Na2MoO4 salt
z
Mostly baseline separation of C1 to C10 hydrocarbons, good baseline separation of cyclopropane from
propylene
z
Accurate quantitations of dienes and halocarbons
z
z
Similar Phases: HP-PLOT Al2O3/"M", GS-Alumina, ZB-Alumina
Temperature
ID(mm)
Length(m)
Film(μm)
0.25
25
5
-60 to 200/250
8325-2505
0.25
30
5
-60 to 200/250
8325-3005
0.32
15
8
-60 to 200/250
8332-1508
0.32
30
8
-60 to 200
8332-3008
0.32
50
8
-60 to 200
8332-5008
0.53
15
15
-60 to 200/250
8353-1515
0.53
30
15
-60 to 200
8353-3015
0.53
50
15
-60 to 200
8353-5015
0.53
60
15
-60 to 200
8353-6015
Limit(℃)
Cat.No.
DA-PLOT MoleSieve
z
Molecular Sieve, Zeolite, 5A
z
Thin and thick coatings for push limit separations, e.g. Ar/O2 separation at 35C, O2/N2 with 15sec
z
Truly immobilized coating and smooth baseline workable for valve switching
z
Preferred column to analyze fixed gases from hydrocarbon streams
z
Elution order, H2, He, Ne, O2, N2, CH4, CO - Regeneration of performance from water/CO2
adsorptions at 250℃ for 3hr
z
z
Noble gas, air from methane, fuel cell, etc
z
Often used with other PLOT columns for fixed gas separation from sample matrix
Similar Phases: HP-PLOT Molesieve, CP-PLOT MoleSieve.
Temperature
ID(mm)
Length(m)
Film(μm)
0.32
5
12
-80 to 300
8432-0512
0.32
15
12
-80 to 300
8432-1512
0.32
30
12
-80 to 300
8432-3012
0.32
15
25
-80 to 300
8432-1525
0.32
30
25
-80 to 300
8432-3025
0.53
5
25
-80 to 300
8453-0525
0.53
15
25
-80 to 300
8453-1525
0.53
30
25
-80 to 300
8453-3025
0.53
15
50
-80 to 300
8453-1550
0.53
30
50
-80 to 300
8453-3050
Limit(℃)
Cat.No.
DA-PLOT GasPro
z
Proprietary Silica base phase
z
Very inert to wide range of gases including sulfur gases and Freons, symmetric peak shapes
z
Truly immobilized coating and smooth baseline workable for valve switching
z
Preferred column to analyze sulfur gases from hydrocarbon streams and air
z
Separation of CO and CO2
z
No effect of water on retention time
z
Regeneration of performance from water adsorptions at 250℃ for 3hr
z
z
GC-MS applicable
Replacement: GS-GasPro, CP-Silica PLOT
Temperature
ID(mm)
Length(m)
Film(μm)
0.32
5
5
-80 to 260/300
8532-0505
0.32
15
5
-80 to 260/300
8532-1505
0.32
30
5
-80 to 260/300
8532-3005
Limit(℃)
Cat.No.
DA-PLOT Q
z
Divinylbenzene polymer particle, non-polar, close to traditional PoraPak Q
z
Truly immobilized coating and smooth baseline workable for valve switching and liquid sample
introduction at low temperatures
z
Highest temperature limit, up to 280/300℃
z
Baseline separation of Air, CO2, water, sulfur gases, hydrogen chlorine, ammonia/light amines from
hydrocarbons such as refinery stream, natural gas, and engine exhaust
z
Excellent separation of C1 to C3 isomers, good separations of C3 to C12, halocarbons
z
Excellent inertness and long lifetime of water/alcohol sample, a good secondary choice of wax column
for alcohol analysis
z
Excellent tolerance to strong acid/base sample without affecting retention time
z
Good anti-oxidation at 280℃ and fast regeneration of performance
z
Observed slight sulfur gas, hydrogen chlorine, and amine absorption
z
Water peak is tailing, but no effect on retention
z
Good choice for analyzing low level solvent residues from wide samples, such as headspace, aqueous,
or organic solutions
z
Preferred column for residue analysis in petroleum industry, pharmaceutical, environmental, chemical
weapon detection
z
Preferred column to analyze polar compounds including oxygenates from hydrocarbon streams:
natural gas, refinery gas, dienes, ethylene, propylene
Replacement: HP-PLOT Q, GS-Q, CP-PoraPLOT Q HT, Supel PLOT Q, Rt-Q, ZB-PLOT Q
Temperature
ID(mm)
Length(m)
Film(μm)
0.32
15
15
-80 to 280/290
8632-1515
0.32
30
15
-80 to 280/290
8632-3015
0.53
15
30
-80 to 280/290
8653-1530
0.53
30
30
-80 to 280/290
8653-3030
Limit(℃)
Cat.No.
DA-PLOT U
z
Divinylbenzene and Ethylene glycol dimethacrylate copolymer particle, polar, close to traditional
PoraPack U
z
Truly immobilized coating and smooth baseline workable for valve switching and liquid sample
introduction at low temperatures
z
Improved separation and selectivity of Air, CO2, water, sulfur gases, hydrogen chlorine, ammonia/light
amines from hydrocarbons
z
Symmetric water peak shape, water elute after C3
z
Excellent separation of C1 to C3 isomers, good separations of C3 to C12, halocarbons
z
Excellent inertness and long lifetime of water/alcohol sample, a good secondary choice of wax column
for alcohol analysis
z
Excellent tolerance to strong acid/base sample without affecting retention time
z
Observed slight sulfur gas, hydrogen chlorine, and amine absorption
z
Good choice for analyzing low level solvent residues from wide samples, such as headspace, aqueous,
or organic solutions
z
Preferred column for residue analysis in petroleum industry, pharmaceutical, environmental, chemical
weapon detection
z
Preferred column to analyze polar compounds including oxygenates from hydrocarbon streams:
natural gas, refinery gas, dienes, ethylene, propylene
Replacement: HP-PLOT U, CP-PoraPLOT U
Temperature
ID(mm)
Length(m)
Film(μm)
0.32
15
10
-80 to 190/200
8732-1510
0.32
30
10
-80 to 190
8732-3010
0.32
30
20
-80 to 190
8732-3020
0.53
15
20
-80 to 190/200
8753-1520
0.53
30
20
-80 to 190
8753-3020
Limit(℃)
Cat.No.
DA-Blood Alcohol Column
z
Proprietary PLOT phase
z
Reliable blood alcohol analysis
z
Tolerance of direct sample injection
z
Excellent confirmation column to other blood alcohol columns using t-butanol as internal standard
ID(mm)
Length(m)
Film(μm)
0.32
7.5
10
Temperature
Limit(℃)
-80 to 260/280
Cat.No.
8932-0710
Equipments, Accessories
and Supplies
z
Column Savor –Online Filter
HPLC Mixer
HPLC Fittings and Tubing
HPLC Injector and Sample Loop
Automated Switching Valve
Automatic Column Selector
HPLC Column Heater
UV Lamps
z
SPE Accessories and Supplies
SPE Vacuum Manifolds
Multipurpose SPE Work-station
SPE-1 Automated Processing Station
Positive Pressure SPE Work-station
Robotic SPE Work-station
Solvent Evaporator
Empty Column and Accessories
Pipettes and Accessories
z
GC Accessories and Supplies
Septa
Injection Port Liners
Ferrules
Column Scribe
1/32” Ultra Low Mass External Unions
z
Filtration Products
Syringe Filters
96 well filtration plates and collection plates
Membrane Filters
Glass Filtration System
Chemical Resistance Guide for Membranes
z
Vials and Caps
Screw Neck Vials and Caps
Crimp Neck Vials and Caps
Plastic Vials (Micro-vials for LC-MS Autosamplers)
Column Savor –Online Filter
Online Filters
z
Totally PEEK polymer wetted surfaces
z
Biocompatible and inert
z
For analytical systems
z
High-pressure applications
Cat.No.
Description
OFP-001
PEEK, ZDV
Inlet Solvent Filters
Bottom-of-the-Bottle Solvent Filters
Cat.No.
10um Filter Assembly¹,for 1/8˝ OD tubing with A•520 replaceable filter cup
AA•550
2um Filter Assembly¹,for 1/8˝ OD tubing with A•522 replaceable filter cup
AA•551
10um Filter Assembly²,for 3/16˝ OD tubing with A•520 replaceable filter cup
AA•552
2um Filter Assembly²,for 3/16˝ OD tubing with A•522 replaceable filter cup
AA•553
Replacement Filter Cups (For AA•550-AA•553 Filters)
10um Stainless Steel Cups,10-pk
AA•520x
2um Stainless Steel Cups,10-pk
AA•522x
HPLC Mixer
Static Bed Mixers
Mixer cartridge
Applicable
Vol.
Flowrate(μL)
50
20-150
SMX050
150
150-500
SMX150
250
500＋
SMX250
Cat.No.
HPLC Fittings and Tubing
One-piece Fingertight Fittings
All of One-piece Fingertight Fittings are designed to be used with 1/16” OD tubing, except the
AM-645(1/32”) and AP-100(1/8”)
Cat.No.
Material
Tubing Size /Nut Threads
Qty
AF-100
Kel-F
1/16”, 10 • 32
10-pk
AF-120
PEEK
1/16”, 10 • 32
10-pk
AF-127
PEEK
1/16”, 10 • 32
10-pk
AF-130
PEEK
1/16”, 10 • 32
10-pk
AM-645
PEEK/Kel-F
1/32”, 6 • 40
10-pk
AP-100
Kel-F
1/8”, 1/4 • 28
each
Two-piece Fingertight Fittings
Cat.No.
Material
Tubing Size /Nut Threads
Qty
AF-200
Delrin
1/16”OD, 10 • 32
10-pk
AF-300
PEEK
1/16”OD, 10 • 32
10-pk
AF-330
Long PEEK
1/16”OD, 10 • 32
10-pk
AF-331
Short PEEK
1/16”OD, 10 • 32
10-pk
AF-200n
Delrin
1/32”OD, 10 • 32
ea
AF-300n
PEEK
1/32”OD, 10 • 32
ea
AF-330n
Long PEEK
1/32”OD, 10 • 32
ea
AF-331n
Short PEEK
1/32”OD, 10 • 32
ea
Connections (Unions and Tees)
Unions
Type
Material
OD
Cat.No.
High Pressure, webbed
PEEK ZDV
1/16"
UNP-001
High Pressure, webbed
Stainless Steel ZDV
1/16"
UNS-001
Tees
Type
Material
OD
Cat.No.
High Pressure
PEEK
1/16"
TEP-001
1/16"
TES-001
High Pressure
Stainless
Steel
PEEK Tubing
PEEK polymer tubing is a good replacement of stainless steel tubing used commonly in HPLC systems. It has many
advantages over the stainless steel tubing, which includes better chemical inertness, better biocompatibility, and
convenience to cut. Additionally, PEEK tubing has a very smooth internal surface, which causes less turbulence than
metal tubing. Unlike other polymer tubing, PEEK tubing can tolerate high pressure (up to 7000 psi) and high temperature
(up to 100℃).
Cat.No.
ID
Pressure Rating
AT1560
0.0025˝( 65 μm )
7,000psi (483bar)*
AT1561
0.004˝ (100 μm )
7,000psi (483bar)*
AT1535
0.005˝ (125 μm )
7,000psi (483bar)*
AT1562
0.006˝ (150 μm )
7,000psi (483bar)*
AT1536
0.007˝ (175 μm )
7,000psi (483bar)*
AT1531
0.010˝ (0.25mm)
7,000psi (483bar)*
AT1565
0.015˝ (0.40mm)
7,000psi (483bar)*
AT1532
0.020˝ (0.50mm)
7,000psi (483bar)*
AT1533
0.030˝ (0.75mm)
7,000psi (483bar)*
AT1538
0.040˝ (1.00mm)
5,000psi (345bar)*
AT1537
0.055˝ (1.40mm)
500psi ( 34bar)*
AT1534
0.062˝ (1.60mm)
4,000psi ( 276bar)*
AT1544
0.080˝ (2.00mm)
3,000psi ( 207bar)*
AT1545
0.010˝ (0.25mm)
7,000psi (483bar)*
AT1546
0.020˝ (0.50mm)
7,000psi (483bar)*
AT1547
0.030˝ (0.75mm)
7,000psi (483bar)*
AT1540
0.042˝ (1.05mm)
5,000psi (345bar)*
AT1539
0.055˝ (1.40mm)
500psi ( 34bar)*
AT1579
0.025˝ ( 65μm )
5,000psi (345bar)*
AT1578
0.035˝ ( 90μm )
5,000psi (345bar)*
AT1576
0.050˝ ( 125μm )
5,000psi (345bar)*
AT1577
0.070˝ ( 175μm )
5,000psi (345bar)*
AT1575
0.080˝ ( 200μm )
5,000psi (345bar)*
AT1580
0.090˝ ( 230μm )
5,000psi (345bar)*
AT1581
0.100˝ (0.25mm)
5,000psi (345bar)*
AT1568
0.150˝ (0.40mm)
5,000psi (345bar)*
AT1569
0.200˝ (0.50mm)
5,000psi (345bar)*
1/16” OD x 5’ L
1/8” OD x 5’ L
1.8 mm OD x 5’ L
1/32” OD x 5’ L
Tubing Holders
Tubing Holders For Polymer Tubing
Cat.No
AP•740
90°Tubing Holder for 1/16˝ OD tubing, polystyrene
AP•741
180°Tubing Holder for 1/16˝ OD tubing, polystyrene
AP•815
90°Tubing Holder for 1/8˝ - 5/16˝ OD tubing, PVT
Tubing Cutters
Tubing Cutters For Polymer Tubing
Cat.No.
Standard Polymer Tubing Cutter* for 1/16˝ and 1/8˝
AA•327
OD tubing
Large Bore Polymer Tubing Cutter* for 3/16˝ - 5/16˝
AA•329
OD tubing
Capillary Polymer Tubing Cutter* for 360μm- 1/32˝
AA•350
OD tubing Includes(1)F•262x10-pack of sleeves
Replacement Blades for A •327, A •329and A •350
AA•328
5-pk
Replacement Sleeves for A •350,0.0155˝ID,Green, for
cutting 360μm OD tubing 10-pk
Alternate Sleeves for A •350,0.021˝ID,Natural, for
AF•262x
AF•264x
cutting 510μm OD tubing 10-pk
Alternate Sleeves for A •350,0.033˝ID,Blue, for
AF•267Bx
cutting 1/32˝ OD tubing 10-pk
Precision Fused Silica Tubing Cutter
AFS•315
Maintenance Kit for FS•315
AFS•315•02
HPLC Injector and Sample Loop
Sample Injector
Type
Description
Cat.No.
Rheodyne 7725 Injector
Standard Analytical Sample Injector; Stainless steel
INJ7725
Standard Analytical Sample Injector; Peek
INJ9725
Preparative Sample Injector
INJ3725
Sample Loop
Type
Cat.No.
5μL Stainless Steel
LPS0005SS
5μL Peek
LPS0005PK
LPS0020SS
20μL Peek
LPS0020PK
LPS0050SS
50μL Peek
LPS0050PK
LPS0100SS
100μL Peek
LPS0100PK
1mL Stainless Steel
LPS1000SS
1mL Peek
LPS1000PK
5mL Stainless Steel
LPS5000SS
10mL Stainless Steel
LPS0000SS
Automated Switching Valve
z
Used for 2D-LC or on-line SPE/LC
z
Editable valve diagram.
z
Flexible in choosing valve number and valve type.
z
The valve types can be 2-position/6-port; 2-position/8-port;
2-position/10- port; 6-position or 10 position.
z
One module can be installed with 1 or 2 valves. Two modules can be
used together to
z
make a 4-valve platform or be sued as two independent modules.
Type
Cat.No.
LC-04SP Valve System
ASV0002-0
Automatic Column Selector
z
Connect up to 6 HPLC columns
z
Controllable by both panel keys and computer
z
Easy key operation for setting method and sequence
z
Compact design and easy access to columns
z
Compatible with any HPLC
z
Compatible with other LC-04SP valves
Cat.No.: AST-006
HPLC Column Heater
Technical specification
Cat.No.
CC-100
Dimensions (H×W×D)
510mm×110mm×65mm
Operating Temperature
Above 5℃～100℃
Temperature Accuracy
±0.1℃
Compatible Column Quantity and Length
2×300mm
Power
220V/50Hz/1A
Weight
4Kg
UV Lamps
Agela Technologies offers UV lamps of the same or better quality as a low cost replacement for many of
those manufactured by original HPLC manufacturers, such as Waters, Agilent, Varian, Shimazu and
Perkin-Elmer.
Original Instrument Part Number
Agela Lamps Part Number
Type of Lamps
l037
AL-610
W
l040A,1040M
ALH-10
D2
1046
AL-890
P/Xe
l047A
AL-610
W
79853A
AL-295
D2
79853C,79854A
ALH-10
D2
79880A,7988lA
ALH-l0
D2
8450A
ALH-l6
D2
8450A
ALH-855
W
8451A
ALH-11
D2
8452A
ALH-12
D2
8453A,8453E
ALH-15
D2
8453A
ALH-13X2
D2
8453A
AL-850
mf
G1306A
ALH-10
D2
G13l4A
ALH-14
D2
Gl315A&B,Gl365A&B
ALH-13
D2
Gl315A&B,Gl365A&B
ALH-13X2
D2
Gl3l5A&B,Gl365A&B
AL-850
W
79875A
Gl32l A
P/Xe
Gl362
W
HP3D/CE
ALH-l4
D2
Series I&II
ALH-10
D2
Shimadzu
Agela Lamps Part
Type of
Number
Lamps
SPD-7A, 7AV
AL-305
D2
SPD-10A, 10 AV, 10AVP
ALH-80
D2
SPD-10 AV, 10AVP
AL-830
W
SPD-M6A, M10A, M10AV
ALH-81
D2
SPD-M6A, M10A
AL-935
W
SPD-M10AV
AL-830
W
SPD-M10AVP
ALH-80
D2
SPD-M10AVP
AL-835
W
SPD-M1A
Varian
Original Instrument Part
Agela Lamps Part
Type of
Number
Number
Lamps
AA200,275,300
AL-230
D2
AA400,600,640
AL-230
D2
AA775,800,875
AL-230
D2
AA975,l275,l475
AL-230
D2
AA3000
AL-230
D2
DMS90
Eclipse
FL-l,2
P/Xe
ALH-910
P/Xe
PDA,1
ALH-80
D2
PDA-2
ALH-80
D2
LC-5000,5500
PDA-2
W
Pro Star
ALH-950
P/Xe
pro Star 310
AL-305
D2
Pro Star 320
ALH-950
P/Xe
Pro Star 330
ALH-53
D2
Pro Star 340,345
ALH-40
D2
Pro Star 360
ALH-910
P/Xc
Pro Star 363
AL-800
Xe
Pro Star 364
P/Xe
Spectra A10,20,30,40
AL-230
D2
Star 9050
AL-305
D2
1JV•1,lA
ALH-950
P/Xe
UV50,100,200
AL-305
D2
VIsta 5500
AL-305
D2
Waters
Agela Lamps Part
Type of
Number
Lamps
420
AL-770
Hg
440,44l
AL-820
Hg
441
AL-720
Cd
441
AL-7l5
Zn
450
ALHA-l
D2 Lamp Ass’y
450
ALH-l
D2 Replacement
470,474
AL-800
Xe
480,48l
AL-205
D2
484
ALH-3O
D2
486
ALH-3l
D2
990,99l,994
ALHA-34
D2 Lamp Ass’y
990,991,994
ALH-34
D2 Replacement
996
ALH-33
D2
2475
ALH-925
Xe
2487,2488
ALH-36X2
D2
2996
ALH-33
D2
LC Module l/3455l System
ALH-3l
D2
40l,403,404
SPE Accessories and Supplies
SPE Vacuum Manifolds
SPE Vaccum Manifold for SPE sample preparation, filtration and elution
are available in 12 and 24 port configurations. These manifolds permit
consistent extraction and filtration results. Multiple sample processing
with these manifolds consist of a clear glass chamber and lid, to which
a vacuum is applied to draw solvents and sample through an SPE
column, cartridge, or disk. The lid is CNC machined, solvent resistant,
low extractable virgin polypropylene. The lid is autoclavable, and will
not wrap. The female Lure inlets and male Lure outlets are molded of
pure polypropylene. Adjustable racks placed in the glass chamber will accommodate a variety of sample
collection vessels, including test tubes, autosampler vials, volumetric flasks, and Erlenmeyer flasks. Eluants
are deposited directly into the collection vessel of choice via polypropylene, or optional stailness steel or
Teflon needles.
Type
Cat.No.
12 port Model
VM0012
24 port Model
VM0024
96well plate Model
VM0096
Multipurpose SPE Work-station
This is specially designed to replace conventional SPE manifolds and solvent
evaporators by an integrated and versatile workstation. It features:
z
Ease for operation: easier to change the collection tubes
z
Flexibility: can be used for vacuum or positive pressure mode;
z
more choices for tube size
z
Multifunction: SPE processing and solvent evaporation
z
Solid: made of aluminum alloy instead of fragile glass
z
Compatible with automated liquid handling systems
Cat.No.: MST-001
SPE-1 Automated Processing Station
z
Designed for sample preparation in trace analysis of food and environmental
samples.
z
Process up to 6 samples per batch without attendance.
z
Up to 5 solvents can be used for column conditioning and multi step elution.
z
Two fractions can be collected for each sample.
z
Accommodate SPE cartridges and traditional cleanup columns.
z
Exhaustive sample injection.
z
Easy operation with only 7 buttons.
z
Small footprint and computer-free operation
Cat.No.: APT-001
Positive Pressure SPE Work-station
Cat.No.
Description
Qty/Pk
APSP-48
Positive Pressure Processor-48 Position
1
APSP-22
Rack for 1 ml SPE Columns
1
APSP-23
1
APSP-24
1
APSP-19
Collection Tube Rack, 12 x 75 mm Tubes
1
APSP-20
APSP-21
APSP-25
Collection Tube Rack, 13 x 100 mm
Tubes
Collection Tube Rack, 16 x 100 mm
Tubes
Collection Vial Rack, 12 x 32 mm
Auto-Sampler
1
1
1
APSP-26
Waste Bin
1
APSP-27
48-Column Sealing Gasket
1
APSP-28
Gas Supply Adapter
1
Robotic SPE Work-station
z
24 well to 96 well reformatting
z
96 and 384 well dispensing
z
Automated Tip changing
z
96 well and 384 well liquid level sensing
z
12 decks for large sample processing
Cat.No.: RST-0096
Solvent Evaporator
Product Features
z
Combination of heating and vacuum for speedy evaporation of a variety of
solvents at relative lower temperature (safe, faster and better for unstable compounds)
z
Simple operation, no programming set-up needed
z
Adjustable gas pressure and flow
z
Multi-size block interchangeable, accepts tubes OD from 11 to 29mm
z
Optional automatic shutoff, for safe and fast parallel enrichment of various samples
z
Small footprint, only 18(W)x16(D)inch bench area
z
Broad customization configurations
z
Economic and reliable
1.Height post
2.Vacuum vent
3.Needle piercing gas manifold
4.Sample tubes
5.Temperature controller
6.System flow controller
Technical Specifications:
Dimension
18(W)x20(H)x16(D) inch
45(W)x50(H)x40(D)cm
Weight
20lb/10kg
Power
90-120VAC, 50-60Hz, 500VA
Mount
Bench stand
Working environment
4 to 40°C, Humidity <80%
Well plate dimension
5(W)x4.5(D)x2(H)inch127(W)x110(D)x50(H)mm
Number of Well Plate
12, 15, 30, 48
Tube OD
11-29mm
Tube length
125-200(mm)
Temperature
RT-150°C
Temperature accuracy
+-1°C
Gas pressure
30psi (2bar) max
Gas flow
0-5liter/min
Needle
Stainless steel, 1.6(OD)x150(L)mm
Gas/vacuum connection fitting
Hose barb 1/8 flow port
Auxiliary vacuum
5-12psi, 200-700mmHg
Description
Cat.No.
Solvent evaporator, including a multi-needle manifold, inert gas flow module,
heater & controller, 120VAC, 1year limited warranty.
GD-101(regular system)
Order multi-well blocks and manifold plates separately!
Solvent evaporator, including individual flow controlled needle manifold,
heater & controller, 120VAC, 1year limited warranty
GD-102-xx, xx is number of wells of block
Order multi-well blocks and manifold plates separately!
Solvent evaporator, 48 or 96well plate format, including purge manifold,
GD-103-48
inert gas heating & controller, 120VAC, 1year limited warranty
GD-103-96
Empty Columns and Accessories
Description
Tube Part No.
Frit Part No.
1mL, 100/pk
050901
050911
3mL, 100/pk
050903
050913
6mL, 100/pk
050906
050916
1mL, 3mL, 6mL column,12/pk
050909
Pipettes and Accessories
Mechanical Pipette (Fixed Volume)
Cat No.
Volume (μL)
A722001
5
A722004
10
A722010
20
A722015
25
A722020
50
A722025
100
A722030
200
A722035
250
A722040
500
A722045
1000
A722050
2000
A722055
5000
Mechanical Pipette (AdjustableVolume)
Single Channel Pipette
Cat No.
Volume (μL)
Increment (μL)
A720005
0.1-2.5
0.05
A720000
0.5-10
0.1
A720020
5-50
0.5
A720080
2-20
0.5
A720050
10-100
1
A720070
20-200
1
A720030
50-200
1
A720060
100-1000
5
A720040
200-1000
5
A720110
1000-5000
50
Cat No.
Volume (μL)
Increment (μL)
A720210
0.5-10
0.1
A720220
5-50
0.5
A720240
50-300
5
Cat No.
Volume (μL)
Increment (μL)
A720310
0.5-10
0.1
A720320
5-50
0.5
A720340
50-300
5
8 - Channel Pipette
12 - Channel Pipette
Pipette Tips
Cat No.
Qty
AD781348
FT10μL
AD781349
FT10μL
AD780006
FT300μL
AD780007
FT300μL
AD780019
FT1000μL
AD780020
FT1000μL
AD780046
FT1200μL
GC Accessories and Supplies
Septa
Septa BTO
z
Extended temperature range, low-bleed
z
Maximun temperature 400℃
z
Virtually eliminates injection-port sticking
z
Preconditioned packaged in glass to prevent contamination
z
Each batch GC-FID tested.
z
Ideal for use with low-bleed, “Mass Sepc” capillary columns
Cat.No.
Description
B02-1002
Septa BTO 5mm Center guide (50/pk)
B02-1040
Septa BTO 3/8" (9.5mm) (50/pk)
B02-1031
Septa BTO 11.5mm Center guide (50/pk)
B02-1001
Septa BTO 11mm (7/16") Center guide (50/pk)
B02-1032
septa BOT 17mm,Center guide
Advanced Green 3
z
True low-bleed, high temperature green septum.
z
Maximum temperature 400 ℃
Cat.No.
Description
B02-1006
Septa AG3 5mm Center guide (50/pk)
B02-1005
Septa AG3 11mm (7/16") Center guide (50/pk)
B02-1041
Septa AG3 3/8" (9.5mm) (50/pk)
Injection Port Liners
Organosilane deactivated borosilicate glass (quartz where notes). Guaranteed inertness for phenols,
organic acids and bases. Glass wool is also deactivated. These capillary injection port liners provide a clean,
non-adsorptive surface for your samples. Regular replacement of the liner protects your capillary columns
from potentially harmful non-volatiles that might otherwise deposit on your columns.
For Agilent Capillary GC Instruments
Cat.No.
Description
Qty.
B02-3004
Liner, 4 mm,79mm length, Single Taper w/ Sil Wool
1/pk
B02-3005
5/pk
B02-3007
10/pk
B02-3008
Liner, 4 mm,79mm length, Double Taper
1/pk
B02-3009
5/pk
B02-3011
10/pk
B02-3012
Liner, 4 mm,79mm length, Single Taper
1/pk
B02-3013
5/pk
B02-3015
10/pk
B02-3019
Liner, 4mm,79mm length, with Silanized Wool
1/pk
B02-3020
5/pk
B02-3022
10/pk
B02-3030
Liner, 2 mm,79mm length, Splitless, Glass
1/pk
B02-3031
5/pk
B02-3032
10/pk
B02-3033
Liner, 2 mm,79mm length Splitless, Quartz
1/pk
B02-3034
5/pk
B02-3036
10/pk
For Perkin-Elmer Capillary GCs
Cat.No.
Description
Qty.
Cat.No.
Description
Qty.
B02-3201
Liner, 4 mm Split, 92mm length ,Autosystem for PerkinElmer GC
1/pk
B02-3202
5/pk
B02-3203
10/pk
B02-3204
Liner, 4 mm Split,92mm length Autosystem w/ Wool for PerkinElmer (ea)
1/pk
B02-3205
5/pk
B02-3206
10/pk
B02-3207
Liner,2mm Splitless,92mm length, Autosystem for PerkinElmer GC
1/pk
B02-3208
5/pk
B02-3209
10/pk
B02-3210
Liner,100mm, Dimple Spliter for PerkinElmer GC
1/pk
B02-3211
SLiner,100mm, Dimple pliter for PerkinElmer GC
5/pk
B02-3212
Liner,100mm, Dimple Spliter for PerkinElmer GC
10/pk
For Varian Capillary GCs
Cat.No.
Description
Qty.
B02-3103
4 mm Open Split w/ Sil. Wool for Varian 1075/1077
1/pk
B02-3104
5/pk
B02-3105
10/pk
B02-3112
Liner, Frit Split for Varian 1075/1077
1/pk
B02-3113
5/pk
B02-3114
10/pk
B02-3115
Liner, 4 mm Open Split, for Varian 1075/1077
1/PK
B02-3116
5/PK
B02-3117
10/PK
B02-3118
Liner, 74mm length, Splitless, Glass for Varian 1075/1077 (ea)
1/pk
B02-3119
5/pk
B02-3120
10/pk
Ferrules
Vespel Ferrules-Sold in Packages of 10
z
Upper temperature limit : 350℃
z
100% high-temperature DuPont polyimide
z
Fairly hard; tends to seal permanently to the column
Straight Ferrules-for tubing with ODs as listed
Cat.No.
1/16" ferrules
B02-2125
1/8" ferrules
B02-2133
1/4" ferrules
B02-2141
Capillary Column Ferrules
1/16" to 0.3mm ferrules
B02-2126
B02-2127
B02-2128
B02-2129
B02-2130
B02-2131
1/16" to 0.4mm -Agilent style
B02-2012
B02-2013
B02-2014
Reducing Ferrules
1/8" to 0.4mm Vespel
B02-2135
B02-2136
B02-2137
1/8" to 1/16" Vespel
B02-2138
1/4" to 1/16" ferrules
B02-2143
1/4" To 1/8" ferrules
B02-2144
Graphite Ferrules—Sold 10/pack Except as Noted
z
z
High-purity Graphite; Preferred for capillary GC use
z
Ideal for glass-to-metal connections; doesn't stick to glass
z
Reusable if not over-tightened
Cat.No.
Description
B02-2101
1/16" Graphite Ferrule
B02-2109
B02-2116
Short Ferrules for Agilent Inlets
B02-2001
1/16"to 0.4mm Graphite Ferrule
B02-2002
B02-2003
B02-2004
B02-2102
B02-2103
B02-2104
B02-2105
B02-2106
B02-2110
1/8" to 0.4 mm Graphite Ferrule
B02-2111
B02-2112
B02-2113
1/8" to 1/16" Graphite Ferrule
B02-2117
B02-2118
B02-2119
B02-2120
B02-2121
B02-2122
B02-2123
B02-2124
Reducing Ferrules
Vespel/Graphite Ferrules –Sold in Packages of 10
z
z
Made from quality DuPont polyimide/graphite blend
z
Recommended for GC/MS interface applications
Cat.No.
Description
B02-2145
1/16" V/G Ferrules
B02-2153
1/8" V/G Ferrules
B02-2160
1/4" V/G
Short Ferrules for Agilent Inlet
B02-2005
1/16" to 0.4mm V/G Ferrules
B02-2006
B02-2007
1/16" to 0.8mm V/G
B02-2146
B02-2147
B02-2148
1/16" to 0.8mm V/G
B02-2149
B02-2150
1/16" to 1.2mm V/G
B02-2154
B02-2155
B02-2156
1/8" to 0.8mm V/G
B02-2157
1/8" to 1/16" V/G Ferrules
B02-2161
1/4" to 0.4mm V/G
B02-2162
1/4" to 0.5mm V/G
B02-2163
1/4" to 6.0 mm V/G Ferrules
B02-2164
B02-2166
1/4" to 1/16" V/G Ferrules
Reducing Ferrules
B02-2167
1/4" to 1/8" V/G
Teflon Ferrules –Sold in Packages of 10
z
z
100% Teflon ;completely inert
Cat.No.
Description
B02-2169
1/16" Teflon
B02-2176
1/8" Teflon
B02-2182
1/4" Teflon
B02-2170
1/16" to 0.3mm Teflon
B02-2171
B02-2172
B02-2173
B02-2174
B02-2175
B02-2177
B02-2178
B02-2179
B02-2180
1/8" to 1/16" Teflon
B02-2183
1/4" to 6mm Teflon
B02-2185
B02-2186
1/4" To 1/8" Teflon
B02-2187
Reducing Ferrules
Column Scribe
Used to cut capillary tubing. Simply pull one smooth edge of the hard ceramic scribe (tilted about 45 degrees)
across the column to lightly score the fused silica .Bend the column opposite the score and the column
separate cleanly.
Cat.No.
Description
B02-1006
Column Scribe,10/pk
1/32” Ultra Low Mass External Unions
For capillary tubing 0.1 to 0.32mm I.D. It has very low mass and can be hand-tightened to seal .Use only
with 1/32” fused silica adapters; metal ferrules cannot be used.
Cat.No.
Description
B02-4001
1/32" Union,Bore 0.25mm
B02-4002
1/32" Union,Bore 0.50mm
B02-4003
1/32" Union,Bore
B02-4004
1/32" 1/32" Fused Silica Tubing Adapter Tubing o.d.<0.25mm
B02-4005
1/32" Fused Silica Tubing Adapter Tubing o.d. 0.25-0.4mm
B02-4006
1/32" Fused Silica Tubing Adapter Tubing o.d.0.5mm
B02-2171
B02-2172
B02-2173
B02-2174
B02-2175
Filtration Products
High Quality – All of our products are manufactured by ISO 9001 registered manufacturers and are
guaranteed to meet our published specification.
Best Value – Our list prices are significantly lower than most brand name prices in the market.
Syringe Filters
Mixed cellulose (acetate and nitrate) syringe
Qty.
Cat No.
0.2μm
100
AS011320
0.45μm
100
AS011345
0.2μm
100
AS012520
0.45μm
100
AS012545
0.2μm
100
AS011320-G
0.45μm
100
AS011345-G
0.2μm
100
AS012520-G
0.45μm
100
AS012545-G
0.2μm
100
AS021320
0.45μm
100
AS021345
0.2μm
100
AS022520
0.45μm
100
AS022545
0.2μm
100
AS021320-P
0.45μm
100
AS021345-P
0.2μm
100
AS022520-P
0.45μm
100
AS022545-P
0.2μm
100
AS031320
0.45μm
100
AS031345
0.2μm
100
AS032520
0.45μm
100
AS032545
0.2μm
100
AS031320-P
0.45μm
100
AS031345-P
0.2μm
100
AS032520-P
0.45μm
100
AS032545-P
0.2μm
100
AS041320
0.45μm
100
AS041345
0.2μm
100
AS042520
0.45μm
100
AS042545
1μm
100
AS042501
filters
13mm Syringe Filters
13mm Syringe Filters with glass prefilter
Nylon syringe filters
13mm Syringe Filters with PP prefilter
PVDF syringe filters
PTFE syringe filters
5μm
100
AS042505
0.2μm
100
AS041320-P
0.45μm
100
AS041345-P
0.2μm
100
AS042520-P
0.45μm
100
AS042545-P
1μm
100
AS042501-P
5μm
100
AS042505-P
Polyethersulfone (PES) syringe filters
Qty
Cat No.
0.2μm
100
AS051320
0.45μm
100
AS051345
0.2μm
100
AS052520
0.45μm
100
AS052545
0.2μm
100
0.45μm
100
0.2μm
100
0.45μm
100
0.2μm
100
AS061320
0.45μm
100
AS061345
0.2μm
100
AS062520
0.45μm
100
AS062545
0.2μm
100
0.45μm
100
0.2μm
100
0.45μm
100
AS051320G
AS051345G
AS052520G
AS052545G
Regenerated cellulose (RC) syringe filters
AS061320G
AS061345G
AS062520G
AS062545G
The Syringe Filters’ Specifications
Description
MCM
Nylon
PVDF
Diameter of
Pore size
Pressure
Bubble Point
Water speed
Air speed
Effective
the filter(mm)
(μm)
rating(bar)
rating(bar)
(ml/min/cm2)
(L/min/cm2)
filter area(cm2)
13
0.22
7
3.52
18
0.65
13
0.45
7
2.2
60
0.65
25
0.22
5
3.52
18
3.9
25
0.45
5
2.2
60
3.9
13
0.22
7
2.80
25
0.65
13
0.45
7
2.5
75
0.65
25
0.22
5
2.8
25
3.9
25
0.45
5
2.5
75
3.9
13
0.22
7
1.20
15
16
0.65
13
0.45
7
0.56
35
20
0.65
25
0.22
5
1.20
15
16
3.9
25
0.45
5
0.56
35
20
3.9
PTFE
13
0.22
7
1.00
24
5
0.65
13
0.45
7
0.63
60
8
0.65
25
0.22
5
1.00
24
5
3.90
25
0.45
5
0.63
60
8
3.90
96 Well Filtration Plates and Collection Plates
Type
Vol
Qty
Cat.No.
Filtration plate
2mL
10
FTPP2002-01
PP frits 20μm
1mL
10
FTPP2001-01
Filtration plate
2mL
10
FTPF4502-01
PTFE membrane 0.45μm
1mL
10
FTPF4501-01
Filtration plate packed with
2mL
10
FTFT1002-01
earth diatomaceous 100mg
1mL
10
FTFT1001-01
Protein precipitation plates
2mL
10
PPPC1002-01
and collection
1mL
10
PPPC1001-01
2mL
10
CLCM0002-01
1mL
10
CLCM0001-01
—
10
CLCM9600-0
Collection plate, round
bottom
96 well cap mats
Membrane Filters
Material: cellulose acetate, nylon
Pore size: 0.45μm
Filter size: 13mm, 25mm, 50mm Customized sizes available upon request
Material
Diameter(mm)
Qty
Cat. No.
Cellulose Acetate
13
100/pk
AM011345
25
100/pk
AM012545
50
100/pk
AM015045
13
100/pk
AM021345
25
100/pk
AM022545
50
100/pk
AM025045
Nylon
Glass Filtration System
The system includes a borosilicate glass funnel, a sintered borosilicate glass membrane support base on
neck, aluminum clamp and borosilicate glass filter flask. It can be used with or without membrane depending
on the application requirement to filtrate organic or aqueous solution. It is also used for liquid degassing
Funnel volume: 300mL, 500mL
Pore size of the glass support: 10μm
Flask volume: 1000ml, 2000mL
Funnel Volume (mL)
Flask Volume (mL)
Qty
Cat. No.
300
1000
ea
AM3100
300
2000
ea
AM3200
500
1000
ea
AM5100
500
2000
ea
AM5200
Chemical Resistance Guide for Membranes
Chemical
Mixed Cellulose Acetate/nitrate
Regenerate Cellulose
Nylon
PTEE
1-hexanol
＋
＋
＋
＋
Acetic acid,10%&25%
0
＋
－
＋
Acetone
－
＋
＋
＋
Acetonitrile
－
＋
＋
－
Aliphatic hydrocarbon
＋
＋
＋
＋
Ammonia,1M
＋
＋
＋
＋
Aromatic hydrocarbon
＋
＋
N/A
＋
Benzene
＋
＋
＋
＋
Boric acid
＋
＋
0
＋
CTC
0
＋
－
＋
Carboxylic acid
＋
＋
－
＋
Chloroacetic acid
－
0
－
＋
Chloroform
－
＋
＋
0
Cyclohexane
0
＋
＋
＋
Cyclohexanol
＋
＋
＋
＋
Diethyl ether
0
＋
＋
0
Dimethyl formamide
－
0
＋
＋
DMSO
－
0
N/A
＋
Alcohol＝＜98%
＋
＋
＋
＋
Acetic ether
－
＋
＋
＋
Vinyl chloride
0
＋
＋
＋
Ethylene glycol
＋
＋
＋
＋
Formic acid, 25%
0
＋
－
＋
N-hexane
＋
＋
＋
＋
Hydrochloric acid,25%
－
＋
－
＋
Isopropyl alcohol
＋
＋
＋
＋
Methanol
＋
＋
0
＋
Nitrous acid,25%
0
＋
－
＋
Pentane
＋
＋
＋
＋
Phosphoric acid,25%
＋
＋
0
＋
Phosphoric acid,45%
0
0
0
＋
KOH,1M
－
0
－
＋
Water solution of inorganic salt
＋
＋
＋
＋
NaOH,1M
0
－
－
＋
Acetylene tetrachloride
0
＋
0
＋
THF
－
＋
＋
0
Toluene
＋
＋
＋
＋
TCA,10%
－
－
0
＋
Trichloro ethane
0
＋
0
＋
Trichloroethylene
0
＋
0
＋
Dimethyl benzene
＋
＋
＋
The mark represent: “＋” = chemical resistance; “0” = limited chemical resisitant; “－” = no chemical resistance ;
N/A= no data.
＋
Vials and Caps
Agela Technologies Inc offers a number of high quality vials, caps, seals and crimping tools. Most of them
are manufactured in Europe and meet the high standard of the chromatographic applications. The difference
of our offering from many other brand name venders is the cost to you. We offer lower price for the same
products as many brand name’ s due to our lean cost structure and we provide personal assist to you to
select right products
2 mL 12 x 32 mm Screw Neck Vials and Caps
Vials
Cat.
Cat.
No.(100/pk)
No.(1000/pk)
Clear
AV1000-0
AV1000-1
Std
Amber
AV1010-0
AV1010-1
Screw neck vials, graduated
Std
Clear
AV1001-0
AV1001-1
Std
Amber
AV1011-0
AV1011-1
Screw neck vials
short
Clear
AV1100-0
AV1100-1
short
Clear
AV1101-0
AV1101-1
short
Amber
AV1111-0
AV1111-1
Screw neck vials
Wide mouth 10/425
Clear
AV1200-0
AV1200-1
Wide mouth 10/425
Clear
AV1201-0
AV1201-1
Wide mouth 10/425
Amber
AV1211-0
AV1211-1
Description
Thread
Color
Screw neck vials
Std
Screw neck vials
Caps and Septa
Description
Thread
Cat. No.(100/pk)
Cat. No.(1000/pk)
Screw cap
Std
AV2000-0
AV2000-1
Screw cap
short
AV2100-0
AV2100-1
Screw cap (wide mouth)
Wide mouth (10/425)
AV3100-0
AV3100-1
2 mL 12 x 32 mm Crimp Neck Vials and Caps
Vials
Cat.
Cat.
No.(100/pk)
No.(1000/pk)
Clear
AV5000-0
AV5000-1
Narrow mouth
Amber
AV5010-0
AV5010-1
Crimp neck vials
Wide mouth
Clear
AV5001-0
AV5001-1
Crimp neck vials, graduated
Narrow mouth
Clear
AV5100-0
AV5100-1
Narrow mouth
Amber
AV5101-0
AV5101-1
Wide mouth
Clear
AV5111-0
AV5111-1
Crimp neck vials
Wide mouth
Amber
AV5200-0
AV5200-1
Description
Thread
Color
Crimp neck vials
Narrow mouth
Crimp neck vials
Caps and Septa
Cat.
Description
Thread
Cat. No.(100/pk)
Crimp cap
Narrow mouth
AV6000-0
AV6000-1
Crimp cap
Wide mouth
AV6100-0
AV6100-1
No.(1000/pk)
Plastic Vials (micro-vials for LC-MS autosamplers)
Description
Material
Vol.
Cat. No.
Snap ring micro-vial32×11.6mm
PP
300μL
AV0003-P
Snap ring micro-vial32×11.6mm
TPX
300μL
AV0003-T
Snap Cap
PP
—
AV0003-C
Septa
Silicone/PTFE
—
AV0003-S

Agela Catalog 2009-2010

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