leveron - in-cosmetics Korea

TOTAL SOLUTION FOR NATURAL COSMETICS
MORE THAN CHEMISTRY
 LEVERON - A
INCI : Lippia Citriodora Leaf Extract
[Description]
LEVERON-A is from lemon verbena leaves. Lemon verbena is a species of flowering plant and native to
western south America. Lemon verbena leaves are herbal plant well-known for their capacity to prevent
oxidative stress, such a chronic inflammation and damage to DNA caused by free radicals.
LEVERON-A contains the stabilized and purified acteoside (verbascoside), which is isolated from lemon
verbena leaves as a main active compound and is known as a powerful anti-oxidative agent.
[Physical Properties]
[Solubility]
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Appearance : Transparent brown liquid
Odor : Characteristic
Specific gravity(d20/20) : 0.950 ~ 1.050
pH : 2.5 ~ 4.5
Water : Soluble
Butylene glycol : Soluble
Glycerin : Soluble
Ethanol : Partially soluble
Mineral oils : Insoluble
[Application]
 Anti-aging skin care by anti-oxidation, anti-inflammation
 Daily stress (sun, bacterial, etc) care
[Formulation Instruction]
 Recommended use level : 0.1 – 10.0%
 Incorporation : Dissolve LEVERON-A into aqueous phase at any temperature
 Stability : stable at room temperature (stable on 45℃ for 12 weeks)
[Research background]
 Maintenance of homeostasis through anti-oxidant activity by intake of lemon verbena leaves
 Inhibition of muscle injury from practicing exercise, oxidative stress, inflammatory cytokines release and
oxidative stress (Eur. J. Appl. Physiol. 2011, 111(4):695-705)
 Increase of blood HDL-cholesterol, decrease of the level of markers for oxidative stress such as
malondialdehyde, protein carbonyl (Scand. J. Med. Sci. Sports 2012, 22:454-461)
 Mechanism
 Increase of GSH-reductase activity (post-translational regulation) : regulation of redox balance by
reduction of oxidized glutathione (glutathione disulfide) (Scand. J. Med. Sci. Sports 2012, 22:454-461 )
 High oxidative activity and inhibition of lipid peroxidation in fat soluble conditions (Food Chem. 2009,
117:589-598)
[Efficacy]
< Anti-aging : Anti-Oxidation >
Acteoside 40ppm (Leveron-A 1%)
Vit.C 40ppm
Trolox 40ppm
Anti-oxidation Control by Multi-Pathway
0.5
100
0.45
75
100
100
75
75
50
0.4
50
50
25
0.35
25
25
0.3
0
Hydroxy radical
0
FRAP
0
SOD
Head office : 3,4th Floor, 11, Hakdong-ro 24-gil Gangnam-gu, Seoul, Korea
E-mail : [email protected] Tel : +82 2 518 7752~3
DPPH
TOTAL SOLUTION FOR NATURAL COSMETICS
MORE THAN CHEMISTRY
 LEVERON – A
INCI : Lippia Citriodora Leaf Extract
[Efficacy]
Reactive Oxidative Stress Inhibition assay
No stress (without UV)
Experimental
(UV Stress + LEVERON-A 0.5%)
UV Stress
ROS (%) Control
120
UVB
100
80
***
60
40
20
***
***
***
20 ppm
10 ppm
5 ppm
(0.5%)
(0.25%)
(0.1%)
***
0
Control
Without
200 ppm
Control
Trolox
ROS (%) Control
Inhibitory effects of acteoside on ROS generated in HaCaT cell after Irradiated UVB
120
100
80
60
40
20
0
**
H2O2
***
Control
Without
200 ppm
Control
***
***
***
20 ppm
10 ppm
5 ppm
(0.5%)
(0.25%)
(0.1%)
Trolox
Acteoside (Leveron A %)
H2O2
Acteoside (Leveron A %)
H202
H2O2500uM
500uM
UV
UVB 60mJ/cm2
< Anti-aging : Collagenase inhibition >
MMP-1
Collagen fibers
Collagen fibers
MMP-1 ng/ml
MMP-2
150
130
110
90
70
50
30
10
-10
20 ppm
10 ppm
(0.5%)
(0.25%)
Acteoside
0.3 ppm
Retinoic acid
(-)con
(Leveron-A)
Normal skin
Aging skin
UV-B 200 mJ
w/o UV
< Anti-aging : Anti glycation>
BSA + Gal
Acteoside
Control
0.5mM
5mM
Reduction of Argpyrimidine(AGEs) formation
by western blot in BSA, MGO model
Inhibition of N-(Carboxymethyl) lysine (AGEs)
by ELISA
Head office : 3,4th Floor, 11, Hakdong-ro 24-gil Gangnam-gu, Seoul, Korea
E-mail : [email protected] Tel : +82 2 518 7752~3