Olympus IMT-2 Inverted Research Microscope Instructions

INSTRUCTIONS
IMT-2
INVERTED
RESEARCH
INVERTED
RESEARCH
MICROSCOPE
MICROSCOPE
This
instruction
manual
has
been
written
for
use
of
This
instruction
manual
has
be n
writen
for
use
of the
the
Olympus
Inverted
Research
Microscope
Model
IM
Olympus
lnvert d
Res arch
Microscope
Model
IMT-2. T-2. Before
Before
putting
the
into
operation,
i:it is is recommended
puting
the microscope
microscope
into
operation,
recom ende
that
you
reed
this
manual
carefully
in
order
(o
that
you
read
this
manual
carefuly
in order
to familiarize
familarize
yourself
with
the
of
so
you
yourself
fufully
ly
with
the use
use
of this
this micwscope
microscope
so that
that
you
may
obtain
optimum
performance
of
may
obtain
optimum
performance
of this
this instrument.
instrument.
OLYMPUS
-
AXM27
USE
IBEFORE
BEFORE
USE
Lh?for 0r operation
OObserve
bserve
the the fol winfotDNirlg
g
points (hÍÌtS cacaret
refuly
1L
.
rijtOn andaid rnaintenc.e
maintena ce:
OpOperation
eration
As
a
tis da pprecison tcIs1Ofl instrument,
always
handle
As
a microscope
microscope
instrument,
always
handle
itit wth
the
care
deserves,
and
avoid
abrupt
motions
with
the
care
it des rves,
and
avoid
abrupt
moti ns
and shocks,
and
shocks.
@
To
ift
from
the
case,
To
lift the
the microscope
microscope
from
the packing
packing
case,
hold oid it
it
at
the
0M
mount
A)
and
the
fluorescence
illuinriator
at the
OM
mount
(A)
and
the
fluorescence
iluminator
mounîin
mounti g
(B).
ththread
read
(B).
(Fig. Fiq. 1)
1)
® Tri
the
To nove
move
the microscope
microscope
Hold
the
microscope
A)
and
the
Hold
the
microscope
by bythe the OM 0M mmount
ount
(A)
and
the
fluorescence
(Bi
or
fluorescence
iluminatoilluminator
r
mmounting
ounti g
ththread
read
(B)
or place
place
your
hands
under
the
base
ai
scope
your
hands
under
the microscope
microscope
base
at the
the micre
microscope
side
(C)
and
back
'O).
side
(C)
and
back
(D).
not
hard
rests,
since
DDo
o
not
grasp rasp thethe olastic
plastic
hand
rest ,
since
theythey area'e not
not
strong
enough
the
microscope.
strong
enough
toto support
sup ort
the
microscope.
0(4
Do
not
use
any
bulb
other
tren
the
by
Do
not
use
any
bulb
other
than
the one
one designated
designated
by
Olympus
(12V5OWHAL
h&ogen
bulb).
Olympus
(12V50WHAL
halogen
bulb).
(5, Avoid
exposure
at
microscope
to
sunhiah,
Avoid
exposure
of the
the
microscope
to direct
direct
sunlight,
high
temperature
dust
and
vibration,
high
temperature
anand
d
hhumidity,
umidty,
dust
and
vibration.
a
0
Fig i
(Model
IMT2-SFR
shown)
(Model
IMT2SFR
shown)
Make
toto use
adjustment
ring
to
the
tension
of
coarse
adjustment
knobs.
(Ori
Make
itit aa point
point
use the
the tension
tension
adjustment
ring
to adiust
adjust
the
tension
of the
the
coarse
adjustment
knobs.
( :Do
rij
the
adjustment
knobs
n
directions
siniultaneouslyj
not rotate
rotae
the coarse
coarse
adjustment
knobs
in the
the opposite
op osite
directions
simultaneously.)
sure
the
line
voltage
selector
switch
's
to
with
the
mains
voltage.
Make
sure
ththat
at
the
line
voltage
sel ctor
switch
is set
set
to conform
conform
with
the local
local
mains
voltage.
0 Make
(L
the
cord
from
the
before
fuse
replacement
Discon ect
the power
power
cord
from
the AC
AC outlet
outle
before
fuse
replacement.
8 Disconnn
c Always ground the microscope.
ground
the
microscope.
0 Always
2.
2.
Maintenance
Maintena ce
@
Lens
clean.
Fine
dust
on
surfaces
should
be
off
of
Lens
susurlaces
rfaces
mustmust alwalways
ays
bebe kept
kept
clean.
Fine
dust
on lens
lens
surfaces
should
be blown
blown
of by
by means
means
of aa hand
hand
blower,
Carefully
wipe
Ott
oil
on
with
gau2e
moistened
with
aa small
amount
blower.
Carefuly
wipe
of
oil or
or fingerprints
fingerpints
on the
the lens
lens surfaces
surfaces
with
gauze
moisten d
with
smal
amount
at
xylene,
or
of
xylen ,
or
a
cleaning
cleani g
medirnediurr
um
((alcohol
alcoh l
andand etherether 33:: 7),
7).
(i@ Do
solutions
toto wipe
the
of
plastic
comoonents,
Do not
not use
use organic
organic
solutions
wipe
the surfaces
surfaces
of various
various
plastic
compone ts.
riot
to
the
Solution.
etc
IfIf spilt,
itit should
be
off
ltIt isis recommended
@ J Be
Be careful
careful
not
to spill
spil
the culture
cult re
solution,
etc.
spilt,
should
be wiped
wiped
of irnmedtately,
im ediately.
recom ende
aa waterproof
cover
optionally
available
toto use
use
waterpo f
cover
optionaly
av ilable.
t:@ When
objectives
are
not
screwed
into
the
apertures,
plugs,
will
When
objectives
are
not
screwed
into
the nosepiece
nosepiec
apertues,
sseal
eal
the the aapertures
pertues
with with dust
dust
plugs,
wwhich
hich
wil
protect
the
lenses
dust,
culture
Solution.
etc.
protect
the
lense
locatedlocatedin in the the lowelower
r
lighlight
t
ppath
ath
ftroni
rom
dust,
cult re
solutron,
etc.
ti@ After
use
should
be
with
the
vinyl
dust
cover
provided
and
stored
¡n
free
from
After
use the
the microscope
microscope
should
be covered
cover d
with
the
vinyl
dust
cover
provide
and
stored
in a
a place
place
fre
from
humidity,
humidty.
CONTENTS
FICATIONS
!2.
STANDARD CONFIGURATIONS
OMENCLATURE
J
EM B LV
r6.
51
5-l
Observation
Procedure
Observation
Procedure
5-2
5-2
Operation
of
Components
....
Operation
of Individual
Indivdual
Compone ts
......
lo
10
11
1
PHOTOMICROGRAPHY
6-1
6-l
Photomicrographc
System
Phot micrographic
System
......
18
18
6-2
6-2
Thking
Pictures
Taking
Pictures
.......
20
20
ROUBLESHOOTING GUIDE
22
I
SPECIFICATIONS
I hem
tern
Specifcaiors
Specifcations
Focus
adjustment
by
of
vertical
rnovemen
Focus
adjustment
by
vertical
movement
of th
the
Microscope
vlicroscope
stard
#sand
riosep}ece
¡n
nosepiec
(stage(stayeis s fixfixed
ed
in positon) osition by
by reans
means
o
coarse
and
fine
adjustneni
knobs;
roller
guide
stroke
of the
the iOaXia
coaxial
coarse
and
fine
adjustment
knobs,
point
on
roler
guide
stroke
(from rrn the
the focal
focal
point
on
surfacc
thetile S1aJe
stage
surface).
8rntri
8m
upwuiward
ard
and and2m 2mmdownward; downwrd;with withreduction reducton
gear, gaî,graduated rduted nrn 'ncre
tncre-
menis
ot
ments
of 2j.L
2g.
Nosepec:
\Josepiec .
Se*upIe.
with
provision
For
the
Nomarsk
slider
attachment.
cetachable.
Sextuple,
with
provison
for mountrng
mountrng
the
Nomarski
slrder
at chment,
detachable.
Stage:
Gtage:
Cro5s-movernnt
stage
Cros -movement
stage
JMT2.SVR
IMT2SVR
xx 200mm.
traversing
area
Sünim
xx SOnim;
with
low
posiwith
low
posi2200mm
0m
20
m,
traversing
area
50m
50 m ;
knobs;
pla!esoutsidedanoeter
1)OmíTi.
ttioned
ioned
cocoaxaI
axial
ccontro'
ontrol
knobs;
2 2 insinwt
ert
plates,
outside
diamet r
1 lOm ,
inside
20mm
and
50mm
respeclively.
inside
diametdiarntrs
rs
20 m
and
50 m
respectively.
Cross-m(iernent staue
MT2SFR
traversn
area
x x 5Orrim;
with
low
posiwith
ow
posi2200mm
0m
xx 200mm,
2O m ,
traversing
area
50&mn
m
50 m ;
uoned
control
knobs
diameir
i 10mm,
tioned
coaxcoaxI
ial
control
knobs;
2 insertnsertplates, pIa. outde
outside
diamet r
1 IOm ,
flexbIe,
ininside
side
diadiameters
met rs
20 20m mmand and50 5Om mnrespectivresjectively,
ely,
lolow
w
flexible.
SquarepÌainstag
Square
plain
stage
IMT2SP
l6Omrr
ouiside
1)0mm,
Substage
insert nsert plate.
plate,
outside
diadianetemet r
10
m.
Substage
160
m
x 220i'nm;
20
m;
provided;
pravison
niechanical
stage
IMT2MVR,
provide ;
provison
for or at ching 1tachnq he
the
mechanical
stage
IMT2-MVR.
Mechamcal
stage
Mechanical
stage
Traversing
right
oor left
Traversing
area araa1 0m 110mmx x72m ; 72mm, attachable
at chable
atat the
the
right
left
side
side
attachment
IMT2.MVR
at chment
IMT2-MVR
on
plain
stage.
on the
the
plain
stage.
for
accommodation
ofof various
culture
vessels
and
PProvision
rovison
for
ac om odation
various
cult re
ves els
and soecimen
specimen
holders.
holders.
source
LLight
ight
source
Illumination:
Ilumination:
holder
FiFilter
lter
holder
Halogen
centering
device
light
inHalogen
bulbbulb 12V5OWHAL,
12V50WHAL,
with with bulb
bulb
centering
device
aand
nd
light
intensive
Vo'tage
indication
by
graph
voitmeler.
tensive
contrcontrol;
ol;
Voltage
indication
by bar
bar
graph
voltmet r.
Provided
filter
holders,
green
interference
and
Provide
with with 4 4 flip-up
flip-up
filter
holders;
gre n
interf ence
fifilter
lter
and
Frosted
frosted
filfilter.
ter.
Condenser
holder
Condenser
holder
t
Observation
Observation
system:
sy tem.
Flip-up,
swing-out
typo;
circular
mountr
corFlip-up,
swing-out
type;
cir ular
mounti g
dovetail oetail for
for slide-in
slide-in
conden
ser:
vertical
movement
on
in on, condenser
centering
denser;
vertical
movement
on rack-and-p
rack-and-pinon,
condenser
centering
knobs:
tension
adjustment
height
adjustment
knob.
knobs;
tension
adjustment
ofof the
the
height
adjustment
knob.
Builtin
magnification
changer,
with
centering
telescope
i1 X-1
.5X.CT.
Built-n
magnifcation
changer,
with
centering
tel scope
X-1
.5X-CT.
Light
path
for
3-setting
positions,
linked
with
focusin9
retcIes
Light
path
for photor-iicrography.
phot micrography,
3-seting
positons,
linked
with
focusing
retiles;
tion
tube
(Bl).
35mm
carrera
COM)
and
multitube
mounting
port
(MIL))
tion
tube
(BI),
35m
camera
(OM)
and
multi-ube
mounti g
port
(MTU).
fu
for observaobserva-
450;
Binocular
tube,
inclined
interpupillary
distance
constant
Binocular
tube,
inclined
45’;
interpu ilary
distance
aadjustment
djustment
fromf rom53 53mm
m
to to 775mm:
5m ;
constant
tube
length
adjustment.
tube
length
adjustment.
Eyepeces
WIIK1OX,
WHK
lOX-H Ctield
number
20)
Eyepiec s
WHKlOX,
WHKIOX-H
(field
number
20)
0M
light
path
OM
light
path:
2.5)<
photo
eyepiece
0M
bayonet
mount.
2.5X
FFK
K
phot
ey prec
bubuiltin;
ilt-n;
OM system
sy tem
bayonet
mount.
Multi-tube
light
path:
provision
for
mounting
the
PM-lO
photomic'ograhic
equipment
directly
Multi-ube
light
path;
provison
for
mounti g
the
PM-10
phot micrographic
equipment
directly
as
well
as
the
multitube
attachment.
as wel
as the
multi-ube
at chment.
Condenser:
Condenser.
Lcry
working
distance
turret
condenser:
N.A.
0.55,
W.D,
21
4X.
lOX,
Long
working
distance
turet
condenser;
N.A
0.5 ,
W.D
21 mmm,
,
liglight
ht
aannuli
n uli
fofur
r
4X,
10X,
?OX
and
40X
objectives
and
empty
aperture:
aperture
ids
diaphragm.
20X
and
40X
objectives
and
empty
apertue;
apertue
irs
diaphragm.
Ultra
long
working
disianç
condenser:
NA,
0,30,
W,D.
Light
annuli
for
lOX,
Ultra
long
working
distance
condenser,
N.A
0.30,
W D. 55mm.
5m ,
Light
an ul1
for 4X.
4X,
10X,
20X,
and
40X
objectives
plus
empty
aperture;
aperture
iris
20X,
and
40X
objectives
plus
empty
apertue;
apertue
Iris diaphragm.
diaphragm.
OObjectives:
bjectives.
Phase
PC
4XPL
Phase
contrascontrast
t
objobjectives
ectives
PC S
S Plan
Plan
4XPL
PCS
Plan
PC
S Plan
1Oii)XPL
XPL
N.A.
0.13.
W.D.
15.5mm
N.A
0.13,
W.D
15.
m
N.A,
0.3O,W,D.
7.5mm
N.A
0.30,*W.D
7.5m
LOCD
Plan
2OXPL
N.A.
0.40,
WO,
3.0
LWD-CD
Plan
20XPL
N.A
0.40,
W.D
3.0 mm
m
with
correction
collar
with
corection
colar
LWO-CD
0,50,
W.lJ.
LWD-CD
Plan Plan4OXPL 4OXPL N.N.A.
A
0.60,
W.D
1.9
rem
1.9
m
with
correction
collar
wrth
corection
colar
u
Dmerisions
Dimensions:
320mm
1W)
mm (D) (0) xx 600mm
(H)
320
m
(W)
x x 395
395m
60
m
(H)
WilVeiqht:
eight:
20.5
kg
- (Outfitted
with
standard
20.5
kg
(outfi ed
with
standard
equipmentQuiPrrtent
)
Eyepoint
405
mm
Eyepoint
405
m
Stage
275mm
Stage
heheight
ight
275
m
2
STANDARD CONFIGURATIONS
Component
Compone t
-
Model
Model
-
IMT-2-11
I MT-2l
1
I MT-2-12
MT-2l
2 IMT-2-21
vlT-2 1
I MT-231
IMT-2-31
H
Microscope
stand,
Microscope
stand,
built-in
magnfcation
changer
wiwith
th
built-n
magnifcation
changer
(1X-L5X.CT),
1X-l
.5X-CT),
25X
bbuiliin
uilt-n
2.5X
ohoto
eyepiece,
phot
ey piec ,
50W 50' halogen
halogen
ilumilluriiiIMT2F
I MT2-F
O
0
O
0
O
O
Power
cord
Power
cord
UYCP
UYCP
O
0
O
0
O
O
inclined
BBinocular
inocular
tube,tube, 45°
45’
inclined
BH2-B145
BH2-B145
O
0
O
0
O
O
-1
Intermediate
Intermediate
tutube
be
IMT2-ATU
I MT2-ATU
O
0
O
0
O
O
Sextuple
revolving
riosepiece
;extuple
revolving
nosepiec
IMT2-RE
IMT2-RE
C
0
o
>
O
0
O
O
50W
halogen
lamp
housing
jOW
halogen
lamp
housing
IMT2-LSH
IMT2-LSH
O
0
O
0
O
0
I
I
O
0
JC12V50''HAL
JC12V50WHAL
O
0
O
0
O
O
I
O
nator,
condenser
homer,
hand
rests
nator,
condenser
holder,
hand
rest
(paired),paired), dust
dust
cover,
filter
43lF550-W45
and
screw
driver
cover,
filter
431F5 0-W45
and
screw
driver
50W
halogen
bulbs.
22 pcs
jOW
halogen
bulbs,
PCS.
Cross-rnoverrtent
Iros -movement
mechmechanical
anical
stagestagewith with righi-hand
right- and
ow
drive
controls
ow
drive
controls
Cross-movement
:ros -movement
mechamechanical
nical
stage stagewith with rigright-hand
ht- and
T
IMT2SVR
I MT2-SVR
low
flexíble
ow
flexible
IMT2-SFR
IMT2-SFR
Square
plain
stage
;quare
plain
stage
IMT2-SP2
IMT2-SP2
O
0
Q
0
Methan
caí stastage
controls
nechanical
ge
at chmentattachmentwith with lowcw drive
drive
controls
IMT2-MVP
I MT2-MVR
O
0
O
0
Long
working
dìstance
turret
condenser
-ong
working
distance
turet
condenser
IMT2-LWCD
IMT2-LWCD
Ultra
(ong
working
dsiance
turret
condenser
Jltra
long
working
distance
turet
condenser
IMT2-ULWCD
IMT2-U
LWCD
z
o
C)
o
z
0
-n
Ö
C
-1
o
z
(J)
O
0
O
0
O
0
II
O
0
O
0
O
0
I
O
0
O
0
O
0
O
0
O
0
O
0
O
0
0
O
O
0
O
0
O
O
O
O
PCSPL1OXPL
PCSPLlOXPL
O
0
O
0
LWDCIJPL2OXPL
LWDCDPL2OXPL
O
0
O
O
LWDCDPL4OXPL
LWDCDPL40XPL
EyepIeces
Eyepiec s
CI)
0
PCSPL4XPL
PCSPL4XPL
Objectives
Objectives
-
WHKIO>(
I: :-, WHKIOXH
O
O
/
O
:
1
O
i
l-
Note:
O0 indìcates
the
compatible
corrponents
for
IMT-2-11/IMT-2-t2/IMT-2.2111MT-2-31.
Note:
indicates
the
compatible
compone ts
for Model
Model
IMT-21
l/ IMT-212/lMT-2 1/IMT-231.
n
3
NOMENCLATURE
Field
Field
irs ii
laver
lev r
diaphragm iapraq
Long
distance
Long
workvrking
ing
distance
Lamp housing
housing
clamøing knob
condensir
condenser
Lamp
Condenser darnoing knob
w
D
I-
Stage
insert
pL3te
Stage
insert
plate
-J
L)
Nomarski
slider
omarski
slider
insertion
slot
insertion
slot
o
Exciter
filter
Exciter
filter
\Eyepiec
z
w
loî
irser1tofl
insertion
slot
z
Eyepiece
Diopter
adjustment
Diopter
adjustment
ring rirj
Observaton
ti,be
Observation
tube
%\
Objective
MuRIube
cnou.tinq
Multi-ube
mounti g
-I~ ~.
adapter
a pTer
Mir
svith
Main
switch
((lMT2
IMT-212
12 shown)
shown)
Mounting thod
Fr optional fluorescence
lamp OuSifl9
Fuse hDlder
Primary cord receptacle
\
B
Line
vottga
sele-ctor
Line
voltage
sel ctor
switch
switch
Ln-p
Lamp
houshöjflg
ing
/
Filter holder
Butb
socket
clamping/
Bulb
socket
clamping/
vertical centeìng knob
Field iris diaphragrrî ler
Lcr
working
distance
distance
working
ong
Bulb
horizontal
centering
Bulb
horizontal
centering
\
condensar
k nob
condenser
Condenser centering
knobs
Light arinulus
centering knobs
Condenser
height
Condenser
height
-.
adjusinient knob
Condenser
holder
Condenser
holder
Observation lube
clamping screw
rarTtpng
knob
clamoina
knob
ntermediate tube
clamping knob
i
Stage
crossmnovemen:
Stage
cros -movement
knobs
knobs
35 mm camera back
-___._ Magnification changer ring
-
Light
path
selector
knob
path
sel ctor
knot
Light
for
binocular
tube
e
BI
: for
binocular
tub{
=: fbfor
0M
3
OM
oa;5mrn 35mm camera
camen
back
MTtJ: f nr mu!t=fliba
attachment
Light excluding shutter knob
Light
excluding
shuter
.
kno
Tenion.adjustmert
knob
adjustment
knob
Tension
/
‘/
C3arse adjusiment knob
Coarse
adjustment
knob
I
/Frne
Fjnp adarl;strnenj
knob
justment
knob
Hand rest clamping knob
I
\
rest
1 Haria
Hand
resr\
Minimum
fine
voltage
Min mum
line
voltage
\ selecîo knob
se’ ctor
knob
Light
intensity
Light
intensity
control
lever
control
lev r
(IMT-2-12
shown
I
( IMT-212
shown)
D
4
ASSEMBLY
Remove
the
Remove
the he adhesive
adhesive
tape raDethat ihî locks
locks
the
sesequential
quential
procedure proceue of
path
it path
light
I
sel ctor
knob
transit.
The diagram
diagram
below
ilustraes
the
Ic.tor knob
inin transit.
The
below
illustrates
th$
as embly. ssernhl.
The
numbers
iinriicate
ndicate
the
as embly
ordorder
er
of various
various
compone ts.
The
numbers
the
assembly
of
components,
dust
caps
before
mounting
components.
I*t Remove
Remove
dust
caps
before
mounti g
compone ts.
CD
Eyepiec
Eyepnce
O
working
(i) Long
Long
working
O
long
working
0’ Ultra
Ultra
long
working
distance
ccnd*mse'
distance
condenser
distance
condenser
distance
condenser
and
0 O Socket
Socket
and
assembly
corcord
d
as embly
O
bulb
0 Halogen
Halogen
bulb
O
@ Filter
Filter
o
0
4
Observation
i ube
O Lamp
Lamp
housing b&i
4
O Intermediate
VJbO
Stuge
cclip
Stage
@
k
Østage insert plate
O
0 Crossmove
Cros -move-
+
1
mflt
sta
ment
stage
i!
Objective
!3
LI
I
O
insert
plate
0’ Stage
Stage
insert
plate
,
0O
’
Plain
stage
Plain
stage
+
0
I
R
n.
I.
Stage
clip
4
o’y,
U1
~
35mm
camera
@35m
camera
back
back
t_l
A
-1
@Adapter Adapter for
for
phot micrographic
çhotomicroqraph
C
atachment
at chment
O
@“Extension Extension plaie
plate
L
lr
Allen®
vrench
Alen@
wrench
Cm
sti
0O
” Mechanical
Mechanical
stage
attachment
at chment
D
Hand
m Power
Pnr mr
r. \rA
G
cord
rest
)
the
0O Mounttng
Mounti g
the Stage
Stage
clamping
screw
) Loosen
Lo sen
ththe
e
clamping
screw
0. cij. (Fig,
(Fig.
2)
2)
) Al
Aligni g gong the
the stocJ
stage
positoni g
gro ve
totO the the positposiiioning
oni g
ppin
in
0,
place
positioning
groove
(k.
place
the
cir ular
mounti g
dovetail
of the
the
sstage
tage
onon the
the
sstage
tage
hholder
older
0.
the
circular
mounting
dovetail
of
(F9.
2)
(Fig.
2)
) As
As
tthe
he
stage
clampinclamping
g
screw screwis istighten d, tiitened, both
both
ends
of the
the
stage
wwill
il
stage
ends
of
stage
be
fastened
rricroscope
stand,
be
fasten d
tightlytightly to
to the
the
microscope
stand.
Fig
22
Fig.
f0 Mounting
tite
Mounti g
the Objectives
Objectives
1)
Looser
the
condenser
the
Lo sen
the
condenser
holhoLdor
der
clamping clampingknob knob0, Q),and andflip flip up
up
the
condenser
holder
3)
condenser
holder
0. , IFig,
(Fig.
3)
tension
ofof the
holder
motion
can
be
the condenser
condenser
holder
tiltilting
ng
motian
can
be ad
ad** The
The
tension
justed
by
clamping
screw
can
be
justed
by means
means
ofof the
the
clamping
screw
@(f) which
which
can
be rotated
rotaed
with
aa coin.
with
coin.
L-1
q
Fg,
33
Fig.
2)
Screw
each
(i)
the
Screw
each
oobjective
bjective
@() info
into Lhø
the nosepiece
nosepiec
@ through
through
the stage
stage
insert
mounting
hole
®
lower
power
soto higher
power.
insert
mounti g
hole
@ inin the
the stage,
stage,
lower
power
higher
power,
in
direciton.
(Fig.
4)
in aa clockwise
clockwise
direction.
(Fig.
4)
3)
Click
the
condenser
back
original
positon,
and
clamp
Click
the
condenser
hholder
older
back
toto the
the
orignal
positon,
and
clamp
with
clamping
knob,
with
clamping
knob.
** Please
note
that
the
lens
ofof the
inin the
Please
note
that
the front
front
lens
the objective
objective
the inverted
invert d
microscope
faces
and
is
more
microscope
faces
upupward,
ward,
and
is exposed
exposed
toto contamination
contamination
more
than
the
of
microscope.
Therefore,
¡fif there
than
the objective
objective
of ari
an upnght
upright
microscope.
Ther fore,
ther
are
empty
openings
in
use
plugs
®
are
empty
openi gs
in the
the nosepiece,
nosepiec ,
use
the the ddust
ust
plugs
0 proprovided
to
dust
into
the
etc.
vide
to prevent
prev nt
dust
oor
r debris
debris
fromfrom falling
faling
into
the prisms.
prisms,
etc.
Fig,
44
Fig.
00 Mounting
the
Stage
Mounti g
the Cross-movement
Cros -movement
Stage
U
insert
plate
1) Stage
Stage
insert
plate
Place
a
stage
¡osen
plate
cjL
iThe
insert
plates
are
Place
a stage
insert
plate
@ on
on the
the stage.
stage.
(The
insert
plates
are
provided
with
inner
diameters
20mm
arid
50mm.
Ir
provide
with
in er
diamet rs
20 m
and
50 m .
It is
is recomrecommended
to
aa stage
plate
with
an
diameter
slightly
mende
to select
sel ct
stage
ininsert
sert
plate
with
an inner
in er
diamet r
slightly
smaller
thtn
that
of
a
culture
vessel
in
order
to
prevent
the
smaler
than
that
of
a cult re
ves el
in order
to prev nt
the vessel
ves el
from
toppling
down)
IF19.
5)
from
top ling
down.)
(Fig.
5)
** The
insert
plate
is
designed
to
thin
so
The
insert
plate
is designed
to be
be very
very
thin
so as
as to
to avoid
avoid
hhitting
it ng
against
objectives
at
magnification
change.
ag inst
ththe
e
objectives
at objective
objective
magnifcation
change.
the
plate
roughly
since
this
may
deform,
I*r Do
Do not
not handle
handle
the nsert
insert
plate
roughly
since
this
may
deform.
2)
CilP
2) Stage
Stage
clip
Attach
aa pair
of
Atach
pair
of stage
stage
cclips
lips
prprovided
ovide
with w]th clampiclamping
ng
screws screws
@ 'ion
on the
the
ste
stage. 9e.
Fg.
55
Fig.
>
E
a
P
i
O
th
Plain
Staje
0 Attachiqg
Ataching
the
Plain
Stage
p1te
1i)
) Insert
Insert
plate
)IMT2-5P21
(I MT2SP2)
guide
pin
AAigruinq
ligni g
tthe
he
guide
pin
@
of the
the
insert
plate
with
the notch
notch
@
of
rsert
platO
with
the
sr.açe,.
66)
tthe
he
stage,
mount mountthe theinsert insertplate. plate, (F(Fig
ig.
in n
Fig,
66
Fig.
2)
Mechanical
Mechanical
stagstage
e
at chmenattachment
t
(I(IMT2.MVR)
MT2-MVR)
the
nmechanical chancl stage
attachrrierit
toto
AAttach
tach
the
stage
at chment
the
right
side
of
the lower
lower
rght
side
of
plain
stage,
the
clamping
tthe
he
plain
stage,
tigtightening
hteni g
the
clamping
screw
@ îi with
with
coin.
(Fig.
screw
aa con.(Fig,
77)
uu
epuiprnent,
such
** If If nono photographic
phot graphic
equipment,
such
as thethe PPM-lOAD
M-1OAD
equipment,
as
equipment,
etc.
ìs
light
exit
etc.
is attached
at ched
to to thethe “MT'MTIJ'
U”
light
exit port
port ofof the
the microscope
microscope
can
be
bbase,
ase,
the the stage
stage
atattachment
chment
can
be attached
at ched
on the
the left
left side
side
of the
the
ori
of
plain
stage.
plain
stage.
it
/
/
Fig.
77
Fig.
3)
Stage
plate
Stage
exextension
tension
plate
Align
the
guide
pins
of
extension
the
positioning
Align
the
guide
pins
of the
the
extension
plate plate0 ® into
into
the
positoni g
holes
in
elairi
stage,
and
attach
securely.
(Fig.
61
holes
in the
the
plain
stage,
and
at ch
securely.
(Fig.
8)
Fig.
88
Fig.
O
the
0 Attachsng
Ataching
the Condenser
Condenser
triserl
the
circular
dovetail
ofof the
into
the
holder.
Insert
the
cir ular
dovetail
thg condenser
condenser
into
the condenser
condenser
holder,
and
9)
and
clampclampwith withknob knob
@ '. IFig.
(Fig.
9)
Fig.
99
Fig.
u
O
the
Housing
IIMT2.LSH)
@ Attaching
Ataching
the Lamp
Lamp
Housing
(lMT2-LSH)
Loosen
housng
and
connect
the
lamp
Lo sen
clampclamping
ing
knobknob 0dj ol
of the
the lamp
lamp
housing
and
con ect
the
lamp
housing
to
collector
tens
sleeve
clamp,
(Fig,
101
housing
to the
the
colector
lens
sle ve
0, ', and
and
clamp.
(Fig.
10)
** The
focusing
position
of
lamp
housing
varies
depending
The precise
precise
focusing
positon
of the
the
lamp
housing
varies
dep nding
upon
the
coidensers
iSee
pages
14
15.
16)
upon
the
condensers.
(Se
pages
14,
15,
16)
O
the
Bulb
0 Mounting
Mounti g
the Halogen
Halogen
Bulb
i1) Press
sprtrrg
Leiers
(@
Pres
ththe
e
spring
lev rs
of the
the
bulb
socket
@
inin the
the
direction
of
bulb
socket
OEt
direclion
of
the
arrow,
and
insert
the
bulb
pins
()
into
the
socket
of
the
arow,
and
insert
the
bulb
pins
@I
into
the
socket
holes holes
0. '.
(Fg.
11
(Fig.
1)
** Use
or
the
bulb.
Use gloves
gloves
or gauze
gauze
toto hold
hold
the
bulb.
2)
in
2) Release
Rel ase
the the lelevers
v rs
and and the the bulbbulb is
is held
held
in position,
positon.
the
with
IfIf fingerprints
** Do
Do not
not touch
touch
the bulb
bulb portion
portion
with bare
bare hands.
hands.
fingerpints
or
are
left
wipe
them
oft;
the
or dust
dust
are
left ori
on the
the bulb,
bulb,
wipe
them
of; otherwise
otherwise
the bulb
bulb
life
reduced.
life will
wil be
be considerably
considerably
reduced.
** Lise
bulb
asas designated
by
(12V5OWHALI,
Use a a standard
standard
bulb
designated
by Olympus
Olympus
(12V50WHAL).
h'
100W
halogen
or
high
power
bulb
isis used,
If aa 12v
12V
IO W
halogen
bbulb
ulb
or other
other
high
power
bulb
used,
the
power
circuit
may
be
the
power
cir uit
may
be damaged.
damaged.
Bulb
to
Bulb
to use:
use:
Standafd
Standard
12V5OWHAL:
12V50WHAL:
Compatibk
Compatible
12V5OWHAL-L:
12V50WHAL- :
Fig.
11
Fig.
1
lite
High
intensity
type;
ef ctive
life
High
intensity
type;
effective
at
condtions
about
50
at rated
rated
conditons
about
50 hrs,
hrs.
Long
life
type,
Long
life
type;
fated
cndltDnc
about
2.000
hrs,
rated
conditons
about
2,0
hrs.
effectivo
life
ai
ef ctive
life
at
O
the
Sockel
and
Assembly
0 Connecting
Con ecting
the Bulb
Bulb
Socket
and Cord
Cord
As embly
Insert
the
socket
lamp
housing
Insert
the
socket
into rito the
the
lamp
housing
and andclamp clamp
with withknob knob
0. ',
fFig.
12}
(Fig.
12)
Connect
plug
(@ to
receptacle
back
micro.
Con ect
thethe cord
cord
plug
to the
the
rec ptacle
at at ththe
e
back
ofot the
the
microscope
stand.
scope
stand.
Fig.
12
O
the
0 Mounting
Mounti g
the Filters
Filters
il1) Press
th
lever
ai
way
down
and
insert
a
into
the
Pres
the
lev r
@
al the
the
way
down
and
insert
a fifilter
lter
@
into
the
jilter
hølder
filter
holder
0. i.
** Hold
the
io to avoid
leaving
fingerprints
Hold
the filler
filter at its
at circumference
its cir umfer nce
avoid
leaving
fingerpints
or
smudges
filter
surface.
(Fig.
131
or other
other
smudges
onon ththe
e
filter
surface.
(Fig.
13)
21
into
the
innermost
filter
holder,
with
the
2) MouMount
nt
thethe frIrosted
osted
filtefilter
r
into
the
in ermost
filter
holder,
with
the
frosted
surface
frosted
surface
facingfacing the the operaoperator.
tor.
MoMount
unt
thethe green
gre n
filter filter into
into
adj&ert
holder
The
2 2 filter
holders
are
to
the
adjacent
holder.
The other
other
filter
holders
are provided
provide
to accom
ac ommodate
additional
filters.
optiortallv
available,
modate
ad itonal
filters,
optionaly
av ilable,
e.g .g 43ND6W45,
43ND6-W45,
4545.LBD-2N. LBD-2N.
Fig
13
Fig.
13
u
Connecting
Con ecting
the the InIntermediate
termediate
TubeTube (IMT2-ATU)
(lMT2-ATU)
Place
tube
on
base,
and
clamp
thethe intermediate
intermediate
tube
on the
the microscope
microscope
base,
and
clamp
screw
Place
screw
Ç
Alen
wrench
Provided,
(Fig.
14)
@ wth
with
Alen
wrench
provide .
(Fig.
14)
P Mounting
the
Tube
Mounti g
the Observation
Observation
Tube
Loosen
insert
the
circular
dovetail
of
Lo sen
the the clamclamping
ping
knobknob @ .t f fuluUV,
y,
insert
the
cir ular
dovetail
of the
the
iintermediate
ntermediate
ttube,
ube,
aand
nd
cclamp.
lamp.
(lFig
Fig.
14)
14)
observaton
into
th
observation
tubtube
e
into
the
Fig.
14
Fig.
14
Inserting
the
Inserting
the Eyepieces
Eyepiec s
@
Insert
eyepiece
(focusable
helicoid)
into
the
right
Insert
ththe
e
ey piec
WHKlOW-tK1OXH
X-H
(focusable
witwith
h
helicoid)
into
the
right
eyepïece
ey piec
sle ve sleeveof ofthe the binocbinocular
ular
tube, tube. aligniaÌignin9
g
the the locatinlocating
g
pin pin @ ®
locating
groove
totu the
the
locating
gro ve@.
T
(Fig. Fig, 15)
15)
Insert
the
eyeoiece
the
left
sleee.
Insert
the
ey piec
WHWHK1OX
KlOX
(without(without hehelicoid)
licoid)
ininto
to
the
left
sle ve.
tb
the
Cord
to
at
Base
@ Connecting
Con ecting
the Primary
Primary
Cord
to the
the Receptacle
Rec ptacle
at the
the Microscope
Microscope
Base
Make
switch
is
off:
then
corirrect
the
primary
Make
susure
re
the the ppower
ower
switch
is turned
turned
of;
then
con ect
the
primary
cord
to
AC
outlet
cord
to the
the
AC
outle.
** Ground
the
to
grounded
device
except
aa
Ground
the microscope
microscope
to aa properly
properly
grounde
device
(except
15
FFig.
ig.
15
gas
pipe).
ltIf necessary,
use
an
cord.
gas
pi e).
nec s ary,
use
an extension
extension
cord.
4V Attaching
the
35
Back
Ataching
the
35m mm Camera
Camera
Back
Attaching
the
Adapter
toto the'MTULight
Ataching
the Photom'crographic
Phot micrographic
Adapter
the
“MTU”
Light Exit Pprt
Exit
Port
U Minimum
line
adjustment
Minmum
line voltage
voltage
adjustment
1)
The
min
irnumvoltage voRe required
for
the
light
The
min mum
required
for
the
light
source
can
be coriconsource
can
be
trolled
by
ofof the
adjustment
dial
troled
by means
means
the line
line voltage
voltage
adjustment
dial
@
provide
provided
on
microscope
right-hand
side).
(Fig.
16)
on rh
the
microscope
babase
se
(at at th
the
right- and
side).
(Fig.
16)
AccIaio
that
the
control
(i
Ascertain
that
the slidin
slidng
control
levover
r
@
is positioned
positoned
close t
is
closest
to
Ilow
voltage),
and
then
activate
the
main
switch.
to you
you
(low
voltage),
and
then
activate
the
main
switch.
1
2)
Fg.
16
Fig.
16
3)
Rotare
voltage
the
isis dimly
Rotae
the the line
line
voltage
adjusadustmenr
tment
dial dial @ .) until
until
the lamp
lamp
dimly
lit
lit.
voltage
adjustment
does
not
affect
the
** The
The minimum
minmum
voltage
adjustment
does
not
afect
the maximum
maximum
intensity
of
intensity
of the
the bulb,
bulb.
the
Rests
a1!) Attaching
Ataching
the Hand
Hand
Rest
Attach
the
rests
on
the
right
Atach
the hand
hand
rest
,z
on
the
right
anand
d
left left sides sides0 I oof the
the micromicroscope
aa coin
(Fig.
17)
scope
basebase,
,
clamp carnp knobsknobs 3 3 wIth
with
coin.
(Fig.
17)
** Do
the
rests
to
the
Do not
not grasp
grasp
the hand
hand
rest
to carry
cary
the microscope.
microscope.
** When
the
temperature
regulator
PM-lo
system
isis atWhen
the color
col r
temperature
regulator
oof
f the
the
PM-10
sy tem
atI
I
Fig
17
Fig.
17
tached
to
base,
or
attachments
are
tached
to the
the microscope
microscope
base,
or other
other
at chments
are juxtaposed
juxtaposed
closely
to
the
rests
can
inin case
closely
to the
the microscope,
microscope,
the hand
hand
rest
can bebe detached
detached
case
they
interfere
with
the
ofof these
atiachments
they
interfer
with
the operation
operation
thes
at chments.
5 OPERATION
5-1
Observation Procedure
Normal
procedure
Normal
procedure
Adjustrent
necessary
Adjustment
nec s ary
fobr
r operation
operation
Relevant
part
Rel vant
part
(page)
bage)
Filter
holders
Filter
holders
Coarse
knob
Coarse
adjuadjustment
stment
knob
114)
(14)
Main
switch
Main
switch
(11)
(1 )
Light
path
selector
knob
Light
path
sel ctor
knob
(4
(4)
Specirnenhokber
Specimen
holder
(12)
(12)
(Prepa ration)
Swing
filters.
Swing
outout tthe
he_
filters.
.
Lower
the
nosepiec .
Lower
the nosepiece.
Switch on the light Source.
.
.
Rotate the light path selector knob to
¿
Bl
t
Place a specimen on stage.
.
(B
(8)
lOX
1 Swing
Swing
inin tthe
he
1 OX objective.
objective.
Rotate
condenser
Rotae
tthe
he
condenser
turet turret toto “ Q
0
@ .
Stop
down
the
iris
pposition.
ositon.
Stop
down
the aperture
apertue
irs dia
diaphragm
for
specimens,
phragm
for low
low contrast
contrast
specimens.
.
. .
. .
.
.
iris
in
Apertue
irs diaphragei
diaphragm
in
. Aperture
condenser
condenser
(14)
F
lnterpupdlary
dstance
lnterpu ilary
distance
aadjust
djust-
I
Lment
ment
. . . . . . . . . . . . . . . . ._.
o
. . Eyepiece
sliding
dovetails
Eyepiec
slidng
dovetails
(12)
1
Diopter
adjustment
Diopter
adjustment
I
. . . . . . . . . . . . . . . . . . . . . . . . . . .
Diopter
adjustment
ring
Diopter
adjustment
ring
(14) 14)
Focuc
. . . . . . . . . . . . . . . . . . . . . . . . . . .
Coarse
Coarse
andarid finefine adjustment
adjustment
knobs
knobs
(14)
(14)
*
1 Focus
Focus
field
iris
FOCUS
onon the
the
field
irs diaphragm
diaphragm
Condenser heght adjusimont
Condenser
magnification
Condenser
centraticentration
on
((with
with
magnifcation
changer
at
CT
position);
bulb
centratiori
changer
at CT
positon);
bulb
centration
'ç
Conderier
height
adjustment
Condenser
height
adjustment
knob
knob
knob
. Condenser
Condenser
ccentering
entering
knob
Bulb
centering
knob
Bulb
centering
knob
CT
ring
CT focusing
focusing
ring
.
114)
(14)
(14)
(14)
(14)
(14)
(14)
(14)
Engage the desired objective.
Enga e
the
desired
objective.
1
+
+
Match
the
condenser
Match
the
condenser
turturret.
et.
[
.
.
Light
annulus
centrat
ion
Light
an ul s
centration
. Condenser
Condenser
tuturret
ret
.
Light annulus
centering
knob
an ul s
centering
knob
. Light
CT
CT
.
1
\Finefr
Fine focus
Aduc1
the
collar
of
Adjust
the correction
corection
colar
of objectives
objectrves
20X
and
40X
according
to
the
20X
and
40X
ac ording
to the thickness
thicknes
of the vessel bottom.
fAdjust
light
intensity.
Adjust
light
intensity.
I
V
(Observation)
Coarse andand finefine adjustment
adjustment
. Coarse
knobs
knobs
.
, ofthev ,~ ~ t~ ~:. . ~,:. ~.,
. . .
.
. . . . . .
Adjust
the
iris
in
Adjust
the aperture
apertue
irs diaphragm
diaphragm
in
brightf4eld,
brightfield.
.
.
(14)
(14)
(15
(15)
(IB)
(15)
(14)
(14)
Correction
collar
Corection
colar
116
(16)
Sliding
voltage
control
lever
Slidng
voltage
control
lev r
ill)
(1 )
Aperlure
iris
Apertue
irs diaphragm
diaphragm
(14
(14)
zp
5-2 Operation of Individual Components
on
Source
0O Switching
Switching
on the
the Light
Light
Source
1)
that
the
selector
switch
1) Ascortain
AscertaIn
that
the line
line voltage
voltage
sel ctor
switch
@
located
the back
back
Iocatd
atat the
of
microscope
with
the
locI
mains
of the
the
microscope
stastand
nd
is i$ set set to to conform
conform
with
the
local
mains
voltage.
tEig.
181
adjust
correctly
by
voltage.
(Fig.
18)
If f not,
not,
adjust
corectly
by means
means
oof
f aa small
smal
screwscrew
dr
driver. ver.
Fg. 18
21
sure
the
sliding
voltage
contol
lever
1.:'
2) Make
Make
sure
tthat
hat
the
slidng
voltage
contol
lev r
@ is
is set
set at
at the
the
close t
closest
position
to
operator
how
voltage),
then
turn
on
positon
to the
the
operator
(low
voltage),
then
turn
on the
the power
power
switch
.... IFigs.
18
switch3.
(Figs.
18
&& 19)
19)
Voltage
and
light
intensity
Voltage
aadjustment
djustment
and
light
intensity
e
As
the
lever
forward.
(he
LED
at
indictçir
"PV,
l
As iou
you push
push
the control
control
lev r
forward,
the
LED
at the
the
indicator
“P”
lights
up
on
the
left
side
ci
voltmeter
t@ to
the
bulb
lights
up
on
the
left
side
of the
the
voltmet r
to indicate
indicate
the
bulb
voltage
between
00 - 3V,
Then,
five
LED
on
side
indicate
voltage
betwe n
3V.
Then,
five
LED
on the
the right
right
side
indicate
z
C
I-
4V12V
inin increments
of
(Fig.
191
4V12V
increments
of 2V.
2V.
(Fig.
19)
cr
w
For
overvoltage
beyond
12V
(at
mains
115V(.
the
extremely
For
overvoltage
beyond
12V
(at local
local
mains
1 5V).
the
extremely
right-tiand
LED
blinks
for
right- and
LED
blinks
for warning.
warni g.
l
o
t* The
tifo
1fif ir it is is used
atat aa
The effective
ef ctive
life ofof a abulb
bulb will
wil bebe prolonged
prol nged
used
voltage
lower
than
raied.
voltage
lower
than
rated.
** For
type
color
film,
use
filter
with
For use
use ofof daylight
daylight
type
col r
film,
use the
the LBD-2N
LBD-2N
filter
with
I
Fi
Fig.
voltage
8V.
voltage
atat about
about
8V.
119
9
O
aa Specimen
on
@ Place
Place
Specimen
on the
the Stage,
Stage.
L
A.
fl. IIMT2SFR) MT2-SFR I
A. Cross-movement
Cros -movement
stagstage
e
(IMTIMT2-SV
2-SVR,
1)
The
traversing
area
cross-movement
The
traversing
area
ofof the
the
cros -movement
sstage
tage
is is 50 50m romx * 50 50mm
m.
Select
plate
with
an
diameter
of
or
Sel ct
thethe irisan
insert
plate
with
an inner
in er
diamet r
of 20
20 mm
m
or 50
50 mm
m
2)
Fig.
20
Fig.
20
according
to
vessel,
and
observation
area.
ac ording
to the
the specimen,
specimen,
ves el,
and
observation
area.
Place
on
stage,
Place
aa specimen
specimen
on the
the
stage.
** If Ifyouyou rotate
or or move
thethe cross-movement
stage
rotae thethe nOsepiece
nosepiec
move
cros -movement
stage
after
focusing
on
iOin a a vessel,
the
surface
of
after
focusing
on the
the specimen
specimen
ves el,
the lower
lower
surface
of
which
isis positioned
more
than
11 mmm above
the
surface,
which
positoned
more
than
above
the stage
stage
surface,
the
front
lens
ofof the
may
hit
the
the
front
lens
the objective
objective
may sometimes
sometimes
hit against
ag inst
the
from
underneath.
(Fig.
201
it it isis necessary
insertnsert plate
plate
from
underneath.
(Fig.
20) Therefore,
Ther fore,
nec s ary
before
rotating
the
nosepiece.
sis
tto
o ascertain
ascertain
itits
s safety
safety
before
rotaing
the
nosepiec .
Iflt there
ther
possibility
to
it Isis safe
to
and
then
rotate
the
aany
ny
pos iblity
to impinge,,
impinge-it
safe
to lower
lower
and
then
rotae
the
riosepiece.
nosepiec .
B.
stage
(IMT2-SP2)
and
mechanical
stage
B. Plain
Plain
stage
(IMT2-SP2)
and
mechanical
stage
aattachment
t chment
(lMT2-MVR)
(IMT2-MVRI
of
vessels
or
slides
11)) Placement
Placement
of various
various
ves els
or glass
glas
slides
Coincido
the
vessel
with
the
Coincide
the
ves el
ccenter
enter
with
the
center
of the
the
scale
(5 m
center
of
XX scale
(55mm
positon)
by
the
position
ofof the
positon)
by adjusting
adjusting
the
positon
the specimen
specimen
holder
0,
holder
(D,
í,
clamp
inin position
with
knobs®,
0, then
then
clamp
positon
with
knobs
0.
((Fig.
Fig.
2211
1)
(See
(Se
b.
Fig.
21
Fig.
21
I
.
13
setup
of
attachments
13 for
for
setup
of stage
stage
at chments
in in dderaili
etail.)
2?
plato
2) ClClck-swp
ick-stop
plate
This
mechanical
This
mechanical
stagstage
e
at chmenattachment
t
permits permitsthe theuse useof ofthe the cli k-sclickstop
top
platos
micro
titre
plaies
arid
the
micro
titre
plate
plates
forfor 96well
96wel
micro
tire
plates
and
the 24-well
24.wel
micro
tire
plate
of
chambers
click
positon
for
well,
asas desired.
of other
other
chambers
inin any
any
cli k
positon
for each
each
wel,
desired.
Install
22)
Instal
ththe
e
cliclick-stop
k-stop
plate plate0. D. (Fig.
(Fig.
2)
Attach
the
click
íj,
the
click
spring
tube®
Atach
the
cli k
0, and
and screw
screw
the
cli k
spring
tube
0 inin a a manner
man er
itit clicks
the
cli ks
the stage.
stage.
Moving
itit clicks
at
Moving
ththe
e
sstage.
tage,
adjust adjustthe thecli k click positonpostton sso
o
ththat
at
cli ks
at the
the
conter
of
well.
center
of each
each
wel.
22
FFLg.
ig.
2
Specimen
holder
Specimen
holder
3)
Opening
Openi g
thethe spring.(oaded
spring-loade
finger fingerof of thethe specimen
specimen
holdholder
er
withwith one
one
hand,
place
hand.
hand,
place
aa sspecimen
pecimen
slide sUdeinside insidethe theholder holder with wiTh the the othother
er
hand.
IFig.
23)
(Fig.
23)
the
comes
in
with
the
ofof the
** When
When
the slide
slide
comes
in contact
contact
with
the bock
back
the specimen
specimen
holder,
slowly
return
the
f finginger
holder,
slowly
return
the spring-loaded
spring-loade
er.
** If Ifthethe springloaded
finger
isis returned
too
it it may
cause
spring-loade
finger
returned
to quickly,
quickly,
may
cause
damage
solution,
damage
to to the the spspecimen
ecimen
slide, slide,or orspil spill the the cuculture
lt re
solution.
the
** lt If the
ofof the
the bottom
bot m
the essel
ves el
is s rorounded
unde
ofoff or
or shaped
shaped
ssimilarly,
imlary,
the
the
sspring-loaded
pring-loade
finger finger maymay sosometimes
metimes
not not ccatch
atch
the vessel
ves el
bot mbottoni,
.
Fig.
23
Fig.
23
Stage
plate
Stage
extextension
ension
plate
4)
The
plain
siege
toto match
the
meThe
plain
stage
isis reduced
reduced
in in its
its width
width
match
the compact
compact
mecbancal
stage
attachment.
To
aa arge
culture
vessel,
connect
chanical
stage
at chment.
To observe
observe
large
cult re
ves el,
con ect
the
extension
side
of
plain
stage.
Wig.
24)
the
extension
pplate
late
toto the
the
side
of the
the
plain
stage.
(Fig.
24)
Fig.
24
Fig.
24
Tube
0O Observation
Observation
Tube
1)
2)
Looking
through
the
binocular
tube,
slide
the
knurled
dovetail
Lo king
through
the
binocular
tube,
slide
the
knurled
dovetail
mounts
®
arid
left
with
both
hands,
until
mounts
@ of
of the
the right
right
and
left eyepieces
ey piec s
with
both
hands,
until
aa perfect
binocular
(Ftg.
25)
perf ct
binocular
visovision
n
is s obtained,
obtained.
(Fig.
25)
IfIf your
interpupillary
distance
setting
your
interpu ilary
distance
seting
isis alreadyalreadyknown, known,set
set
it iton
on the
the
between
the
sscale
cale
@ () located
located
betwe n
the eyepieces.
ey piec s.
Fig.
25
Fig.
25
3)
Diopter
adjustment
Diopter
adjustment
the
light
path
selector
pos1ion.
aa
. Rotate
Rotae
the
light
path
sel ctor
knobknob to to the the “OM"0M"
”
positon.
b.
through
the
focusable
eye,
b. Looking
Lo king
through
the
focusable
eyeyepiece
piec
with with thethe right
right
ey ,
4
Fig.
26
Fig.
26
rotate
the
heliCoid
eyepiece,
frarre
reticle
rotae
the
helicoid
rinring
g
@® on
on (he
the
ey piec ,
untiluntil the
the
frame
reticle
can
(Fig.
25)
can
bbe
e shsharpty
arply
focufocused.
sed.
(Fig.
25)
Then,
looking
left
eyepiece
left
eye,
rotate
Then,
lo king
ththrough
rough
ththe
e
left
ey piec
withwith the
the
left
ey ,
rotae
the
diopter
ring
(@ until
the
lirses
the
diopter
ring
until
the cross
cros
lines
cacan
n
bebe sharpsharply
ly
rerecogniied
cognized
as
26)
as twotwo separate
separ te
lines.lines, (Fig.
(Fig.
26)
C
z
?
3
Setup
in
Setup
oof
f the
the rnecharncal
mechanical
stastage
ge
at chmattachment
ent
IMIMT2-MVR
T2-MVR
in detail
detail
®®
c
0:T:i AtachAttaching
ing
the the cli kclick-stop
-stop
plaplate
te
to to tthe
he
mechanical
stage
IMT2-MVR
mechanical
stage
IMT2-MVR
-ST:
11)
) Loosen
Lo sen
the thecli k-stopclick-stopknob knob0. D.
(Diag.
1)
(IJiag,
1)
the
click-stop
notches
c@ with
22J
) AligAlign
n
the
cli k-stop
notches
with
the
cli k-stop
knob.
the
click-stop
knob.
Pitch;
625mm
frfor 6Owll
Terasaki
plate
Pitch
:
6.35m
60.wel
Teras ki
plate
9mm
9m
tOT
for
96-wel 96-wellmicrotiermicrotter pate
plate
plaie
should
be
with
** The
The click-stop
cli k-stop
plate
should
be oriented
oriented
with
the longer
longer
marginal
the
marginal
portion
(betwe n
the plate
plate
end and
and groove
gro ve)
at the
the
ooperators
perator’s
portion
t@ (between
the
end
I at
righihand
side
(in
oror closeSt
right-hand
side
(in the
the XX direction)
direction)
close t
tto
o the
the
operator
operator
(In
Y
(in the
the
Y direction}.
direction).
(Diag.
1)
(Diag.
1)
I-l
Diag.
i-y tighten
33)
) Slight
Slightly
tighten
the
cli k-stop
plate
clamping
knobknob @ 4) with
with
care
the
click-stop
plaie
clamping
care
1
that
the
click-stop
plate
can
be
slightly
until
the
that
the
cli k-stop
plate
can
be moved
moved
slightly
until
the
cli k-stop
clickstop
into
the
notch,
kknob
nob
@ 'i) fits
fits
into
the
notch.
(Diag.
1t)
)
IDiag.
Mounting
the
mechanical
stage
plain
Mounti g
the
mechanical
stage
on on the
the
plain
stage
IMT2-SP
stage
IMT2-SP
@
1)
2)
Rotaie
Y-axis
control
knob
clockwise
all
Rotae
tthe
he
Y-axis
ddrive
rive
control
knob
clockwise
al the
the
way.
way
Place
stage
plain
stage
in
Place
the the mechanical
mechanical
stage
onon the
the
plain
stage
in aa manner
man er
that
the
that
the
back
-@
of
mechanical
stage
iis flush
back
of the
the
mechanical
stage
flush
witwith
h
that
@ of
of the
the plain
plain
that
®
stage.
IDiag,
1)
stage.
(Diag.
1)
3)
96 weIl
microtiter
olate
Tighten
two
clamping
knobs
at
&of the
Tighten
two
clamping
knobs
at the
the underside
underside
the mechanical
mechanical
stage
right
side
of
stage
with
aa coin. in
stage
to to thethe lower
lower
right
side
of the
the plain
plain
stage
with
®
specimen
vessels
0 Use
Use ofof various
various
specimen
ves els
Diag. 2
ves vesiel
el
onon th
the
11)
) Place
Place
a a sspecimen
pecimen
stage
as as shownsiown in
in Diag.
Diag.
through
stage
22 through
6.
6.
** For
microtiter
plate
oror SO-well
Terasaki
For use
use ofof a a96-well
96-wel
microtier
plate
60-wel
Teras ki
plate,
plate,
align
the
right
and
sliders
align
the
rightand left-hand
left-hand
sliders
@( && t @ with
with
the index
index
the
marks
marks
0 on
on the
the XX axis
axis graduations
graduations
as shown
shown
in Diag.
Diag.
2, and
and
as
in
2.
:
each
knob
4?.
cclamp
lamp
each
slidslider
er
cclamping
lamping
knob
0.
@
cfltratOn
Centration
ofof the
the clickstop
cli k-stop
plate
plate
(96-well
(96-wel
microtiter
microtier
plate
plate
and
and
6060well
Terasaki
plate)
wel
Teras ki
plate)
1)
Terasaki
plate
tYag, 3
2)
Lo king
through
the microscope
microscope
ey piec s,
rotrotate
ae
the
low
drive
Looking
1hícuh
tne
eyepieces,
the
low
drive
control
knobs
until
the
ofof the
ioto bebe observed
cotncdes
control
knobs
until
the center
center
the weil
wel
observed
coincides
with
the
center
field
of
with
the
center
oot
f the
the
field
of view.
view.
Tiy!iten
the
clamping
knob
Tighten
the
clamping
knob
@
of the
the
cli k-stop
plate.
(Diag.
1)
of
click-nop
plate.
(Diag.
1)
fJ
3Omm-35mn
dia
Petri
dkh
30m -35m
dia.
Petri
dish
Dg.
44
Diag.
6Omm-93
mm ddia.
Petri
dish
60m -90m
ia.
Petri
dish
Diag.
55
Diag.
Sude
gla
Slide
glas
Diag,
66
Diag.
and
Fine
Adjustment
Knobs
0O Coarse
Coarse
and
Fine
Adjustment
Knobs
1)
Bring
the
objective
as
as
Bring
the
objective
as dose
close
as possible
pos ible
to the
the
specimen
without
to
specin-en
without
thing and
focus
on
roughly
by
of
ttou
ouching
and
focus
on the
the specimen
specimen
roughly
by means
means
of the
the
coarse
coarse
adjustment
tine
focus
with
the
fine
adjustment
knobs knobs0, D, then
then
fine
focus
with
the
fine
adjustment
adjustment
cI
(Fig.27)
krohsi3
knobs
0.
(Fig.
27)
Tension
adjustment
of
adjustment
knobs
Tension
adjustment
of coarse
coarse
adjustment
knobs
Wnle
the
coarse
adustment
motion
isis normally
stiff
While
the
coarse
adjustment
moti n
normaly
stif
and
heavy,
and
heavy,
itit
is
adjustabfe
either
heavy
or
movement
dependìng
is freely
fre ly
adjustable
fotor
r
either
heavy
or lghi
light
movement
dep nding
the
observer's
preference,
adjust
the
tension,
rotate
the
oon
n
the
observer’s
pref rence.
TTo
o
adjust
the
tension,
rotae
the
tendirection
of
arrow
to
the
ssien
ion
adadjustment
justment
ring ring 8 ®' in
In the
the
direction
of the
the
arow
to tighten
tighten
the
tn
F
Fig.
27
27
coarse
(Fig.
2?)
coarse
adjuadjustment
stment
kknpbs
nobs.
(Fig.
27)
** The
motion
isis not
The fine
fine adjustment
adjustment
moti n
not adjustable.
adjustable.
** Be
careful
not
to
forcbly
rotate
the
coarse
adjustrnerit
Be careful
not
to forcibly
rotae
the
coarse
or or fne
fine
adjustment
knobs
knobs
ag against
inst
the theup er upperor orlower lowerlimt limt of
of the
the focusing
focusing
range.
range.
t* DoDo not
the
and
not rotate
rotae
the coarse
coarse
and fine
fine adjustment
adjustment
knobs
simultaneknobs
simultane
ously
to
any
to
adlustments.
ously
to avoid
avoid
any damage
damage
to focusing
focusing
adjustments.
O
0 Condenser
Condenser
Centration entration
The
certerinq
ope-rations
of
working
distance
condenser
The
centering
operations
of the
the long
long
working
distance
condenser
and
and
the
ultra
long
working
distance
condenser
are
the
the
ultra
long
working
distance
condenser
are
the
ssame
ame
asas folfollows.
ws.
turret
(Fig.
281
1) Set
Set
ththe
e
turet
@ D to
to tne
the
” 0
@I
” position
positon.
(Fig.
28)
2) Bring
Bring
ththe
e
sspecimen
pecimen
into into focusfocus by by mmeans
eans
of of the the 10XlOX obeclive.
objective.
the
aperture
iris
* It lis recommended
recom ende
to to sstop
top
ddown
own
the
apertue
irs diaphragm
diaphragm
0 fF
focusing
on
specimen,
(Fig.
281
ffor
or easier
easier
focusing
on an
an unstained
unstained
specimen.
(Fig.
28)
O?
C
* lt
iris
diaphragrr
by
means
iris
dia
331
) StopStop dowdown
n
thethe ffield
ield
irs
diaphragm
by
means
of of tthe
he
fifield
eld
irs
diaand
adjust
the
condenser
height
until
the
phragm hraym lever
lev r
@
and
adjust
the
condenser
height
until
the image
image
cf
diaphragm
cari
sharply.
of the
the field
field
diaphragm
can bebe observed
observed
sharply.
(Figs.
28,
29)
(Figs.
28.
29)
** Holding
the
condenser
knob
with
the
hand,
rotate
Holding
the right
right
condenser
knob
with
the right
right
hand,
rotae
Fig.
26
Fig.
28
left
knob
until
the
ofof the
height
tthe
he
left condenser
condenser
knob
until
the tension
tension
the condenser
condenser
height
adjustment
knobs
is
to
preference.
adjustment
knobs
is adjusted
adjusted
to your
your
pref rence.
4)
the
mage
ofof the
diaphragm
into
the
Of
field
4) Briny
Bring
the
image
the field
field
diaphragm
into
the oenter
center
of the
the
field
means
condenser
centering
knobs
bby
y
means
oof
f the
the
condenser
centering
knobs
0. 'a'. (Figs.
(Ftgs.
28, 28. 29) 2)
Reooen
the
diarthraçm
the
small
pinhole
image
dia.
Re-open
the
diaphragm
uuntil
ntil
the
smal
pinhole
image
oof
f the
the
diaphragni
becomes
phragm
becomes
aa larger
larger
polpolygonal
ygonal
aarea
rea
aroundaround the the perperiphery
phery
oot
f the
the
field,
For
use
open
the
to
field.
For practical
practi al
use, slightly
slightly
open
the diaphragm
diaphragm
to circumscribe
cir umscribe
the
field
01
the
field
of view.
view.
Fig.
29
Fig.
29
O
0
Bulb
Centration
Bulb
Centration
A'ter
the
optical
setue.
ce-nIer
the
halogen
bulb.
After
the complete
complet
optical
setup,
center
the
halogen
bulb.
the
1) Rotate
Rotae
the
mmagnification
agnifcation
changer changerdial dial@to Ø)topositon pOsition
“‘CT”. "CT. (Fig.30)
(Fig.
30)
2)
Rotate
the
focus
ring
to
on
the
exit
pupil
of
lOX
Rotae
the
focus
ring
@
to focus
focus
on
the
exit
pu il
of the
the
10X
objective
(located
at
plane
asas the
annutus
objective
(located
at the
the same
same
plane
the phase
phase
an ul s
of f The
the
phase
the
light
arinulus
ofof the
contrast
condenser
phase
oobjective,
bjective,
the
light
an ul s
the phase
phase
contrast
condenser
or
aperture
iris
or The
the
apertue
irs diaphragm).
diaphragm).
(Fig. Fiq. 30)
30)
Fig.
30
Fig.
30
:
i
3)
up
frost
ftlter
so
the
image
FFlip
lip
up me
the
frosted
filter
so that
that
the
image
of of thethe bulbbulbJiamenî
.filament
@
31)
ccan
an
bbe
e obobserved,
served.
(Fi(Fig
g.
31)
4)
Locsening
housing
clamptng
knob,
Lo seni g
thethe amp
lamp
housing
clamping
knob,
move
the
lamp
housing
move
the
lamp
housmg
in
axial
direction
until
ihe
image
isis brought
into
in the
the
axial
direction
until
the filament
filament
image
brought
into
focus.
focus
®
** Repeat
this
whenever
the
working
an
long
Rep at
this adjustment
adjustment
when ver
the tong
long
working
and i ultra
ultra
long
working
distance
condensers
are
interchanged.
working
distance
condensers
are
interchanged.
with
the
* if it
it isis necessary
nec s ary
to to increase
increase
the the light light iintensity
ntensity
with
the phase
phase
40X,
move
the
housing
so
the
image
oobjective
bjective
40X,
move
the lamp
lamp
housing
so that
that
the filament
filament
image
fills
the
of
anrsulus.
IfIf you
the
ccompletely
omplet ly
fil s
the image
image
of the
the light
light
an ul s.
you change
change
the
objective
40X
to
4X,
at
this
stage,
you
will
note
that
the
light
objective
40X
to 4X,
at this
stage,
you
wil
note
that
the
light
intensity
will
reduced
periphery
field.
intensity
wil be
be somevviiat
somewhat
reduced
at at ththe
e
periphery
oof
f the
the
field.
* If
5)
6)
Center
the
filament
image
by
of
bulb
centering
Center
the
filament
image
by means
means
of the
the
bulb
centering
knobs.
knobs.
Re..engage
Re- nga e
the thefrosted frosted filter.filler.
Fg.
31
Fig.
31
O
Annulus
@ Light
Light
An ul s
Centration entration
centeriny
adjustment
pertains
long
working
and
TThis
his
centering
adjustment
eequally
qualy
pertains
toto the
the
long
working
and
ultra
long
working
distance
condensers
ultra
long
working
distance
condensers.
** The
microscope
adopted
the
centration
system
of
The 1MT-2
IMT-2
microscope
adopted
the individua)
indivdual
centration
sy tem
of
each
light
annulus
each
pphase
hase
an ul annulus,
s,
so sothat that strict strict cencentration
tration
of of the
the
light
an ul s
can
be
each
objective.
Therefore,
inatch
the
light
can
be achieved
achiev d
wiwith
th
each
objective.
Ther fore,
match
the
light
annulus
io
the
phase
objective
magnification
whenever
objectives
an ul s
to the
phase
objective
magnifcation
when ver
objectives
are
changed,
recentration
isis not
necessary
are
changed,
anand
d
re-centration
not
nec s ary
onceonce the the initiai
intial
centration
has
accomplished.
centration
has been
be n
ac omplished.
** Recentration.
however,
'sis required
when
the
bottom
of
Rec ntraion,
howev r,
required
when
the
bot m
of aa culture
cult re
vessel
fiat.
ves el
is is nonoi
t
flat.
Fig.
32
Fig.
32
** This
is is applied
toto objectives
from
This centralien
centration
ap lied
objectives
from
low
to
magnifilow
to high
high
magnifcations.
cations.
in
desired
objective
and
focus
on
Swing
in the
the
desired
objective
and
focus
on the
the specimen.
specimen.
1) ) Swing
2)
the
magnification
CT
position.
(Fly.
32)
Rotae
the
magnifcation
chandanger
ger
tto
o the
the
CT
positon.
(Fig.
32)
2) Rotate
'n
3)
Focus
ìníiuJus
'.@
Focus
ocn
n rc
the priascphase
an ul s
uf
by ;y nans
means
of the
the
focustixcs ring ring0.
j
(Figs. Fiçj,
32.
32,3 ) 331
Rotate
condenser
the
magnification
of
Rotae
tIhe
he
condenser
turetturret until
until
the
magnifcation
of the
the objective
objective
engaged
enga ed
ap ears apoearsin inthe thefront. front
4)
33
FFig
ig.
3
5)
Press
Pres
the the lightlight annulus
an ul s
centecentering
ring
knobs knobs@ ® aand
nd
rorotaie
tae
tthere
hem
uuritI
ntil
both
annuli
are
and
slowly
disengage
both
an uli
are concentric
concentric
and supecimposed.then
superimposed,
then
slowly
disenga e
the
centering
knobs
C4).
CFig.
34
the
centering
knobs
@
(Fig.
34)
¶
C
7
r
C
Fig.
34
Fig.
34
6)
the
magnification
changer
6) Rotate
Rotae
the
magnifcation
changer
@
to
"iX"
position,
and
to the
the
“IX”
positon,
and
observe
the
phase
effect.
32,
observe
thethe specimen
specimen
to to check
check
the
phase
ccontrast
ontrast
ef ct.
(FigsFigs.
.
32,
35)
35)
Fig.
35
Phase
contrast
O
of
Coflar
ofof the
LWD-Ct)
Plan
20X
and
@ Use
Use
of the
the Correction
Corection
Colar
the Objectves
Objectives
LWD-CD
Plan
20X
and
40X,
40x.
After
coarse
and
tine
adjustments,
rotate
the
collar,
keeping
After
coarse
and
fine
adjustments,
rotae
the correCtion
corection
colar,
ke ping
the
specimen
resolution
isis obtained.
Proper
the
specimen
in in fine finp focfocus
us
untiluntil optirruni
optimum
resolution
obtained.
Proper
use
collar
isis specially
effective
toto prevenT
the
deterioration
of
use
oof
f this
this
colar
specialy
ef ctive
prev nt
the
det rioation
of
objective
resolution
caused
uneven
ththe
e
objective
resolution
caused
by by ththe
e
unev n
thickthickness
nes
of
cf variovaous
us
petri
dishes,
culture
bottles,
etc,
petri
dishes,
cult re
botles,
etc. (Fig.
(Fig. 3611
36)
** The
collar
isis effective
with
a a vessel
bottom
from
O0 up
The correction
corection
colar
ef ctive
with
ves el
bot m
from
up
to
inin thickness,
to 2mm
2m
thicknes .
thickness
of
bottom
isis known
i1) ltIf the
the
thicknes
of the
the vessel
ves el
bot m
known:
Match
the
correciion
collar
toto the
ofof the
bottom
by
Match
the
corec;tion
colar
the thickness
thicknes
the vessel
ves el
bot m
by
the
collar
scale
provided.
the
colar
scale
provide ,
Fg.
36
Fig.
36
2) lIthe
thickness
of
the vessel
is
If the
thicknes
of the
ves el
bot bottom
m
is unknown
unk own:
Tie
optimum
position
for
the
correction
collar
The
optimum
positon
for
the
corection
colar
can
be obtained
obtained
can
be
tram the
imago
resolutkn,
After
focusing
adjustment,
from
the
image
resolution.
After
focusing
adjustment,
if a satis-
2)
i
a satis-
factory
sharp
image
obtained,
rotate
the
factory
sharp
image
is s not
not
obtained,
rotae
the
corection
colar
correction
collar
to
the
right
and
left
so
thai
you
can
compare
the
to
the
right
and
left
so
that
you
can
compare
the images
images
aat
t both
both
sides. Reset the collar to the better image then starting
sides.
Res t
the
colar
to
the
bet r
image;
then
staring
ffrom
rom
this
this
position,
further
rotate
The
cedar
to
right
Positon,
further
rotae
the
colar
to the
the
right
anand
d
left left until utìl both
both
images
can bbe
obtained
for
images
can
e
obtained
for
comparison
withwith each
each
other.
AS you
YOU
comparison
other,
As
repeal
Ihis
procedure
several
limes,
rep at
this
procedure
sev ral
times,
you
have
to fine
fine
focus
each
you
have
to
focus
each
time
the
corrtiÖr1
coller
isis ro1ered,
time
the
corection
colar
rotaed.
4:::
Q Use of Iris Diaphra9ms
0
Use
1)
2)
Fig. 37
r
Fig.
37
(As seen Through eyepiece tube.
(As withse n eyepiece
through
ey piec
tube,
removed,)
.
J
'I
with
ey piec
of Iris
Diaphragms
Field iris diaphragm
Field
irs
diaphragm
The
field
iris
diaphragm
controls
the
diameter
of
ray
bundle
The
field
irs
diaphragm
controls
the
diamet r
of the
the
ray
bundle
impinging
on
the
specimen
surface
and
thus
increases
image
definiimpinging
on
the
specimen
surface
and
thus
increase
image
defintion
arid
reduces
glare
tion
and
reduces
glare.
Aperture
iris
diaphragm
Apertue
irs
diaphragm
In
order oto achieve
optimum
obGctive
performance
In order
achiev
optimum
objective
perfomance
inin bri9htfield.
brightfield,
the
opening
of
the
aperture
iris
diaphragm
should
be
the
openi g
of
the
apertue
irs
diaphragm
should
be matched
matched
to
to
the NA,
of
the objective
in
ltIt isis often
preferable,
however,
to
the
N.A
of the
objective
in use.
use.
often
pref rable,
howev r,
to
down
the
diaphragm
by
about
70%
sstop
top
down
the aperture
apertue
diaphragm
by
about
70%
to to 80%
80%
oof
f the
the
objective
N.A
(Fig.
37)
objective
N.A
(Fig.
37)
removed.)
Q Filters
CD
Filters
Optimum
use
of
proper
filters
enhances
the
effective
observation
and
Optimum
use
of
proper
filters
enhances
the
ef ctive
observation
and
photni icrography.
phot micrography.
Fi1J
No.
Dittuion
Drfusron
2
Interference
Interf ence
Dsjgation
/ 45WF
45WF
clirunates
uneven
ilumination,
Elimnates
unev n
ilumination.
43-1F550-W4b
43-l
F5 0-W45
Enhances
phase
Enhances
phase
cocontrast.
ntrast.
43ND25-W45
43N
D25-W45
43ND62A45
43N
D6-W45
Reduces
intensity
Reduces
lighlight
t
intensity
wwithout
ithout
changing
color
ternperatue
changing
col r
temperature.
(gre n)
jIgreen)
Neutral
Neutral
density (grey)
4
density
(grey)
Light balancing 45-LBD-2N
45-LBD-2N
Light
6
*Heat
bal ncing
Heat absorbing45-HA 4HA
absorbing
For
color
ohotomicrographv
For
col r
phot mrcography
with
daylight
film,
with
daylight
film.
#bsos
heat
waves
Absorbs
heat
waves
760
rim
760nm
and
higher
to
the
and
higher
to protect
protect
the speci.
specimen,
men.
*This
This filter is built in the 1M12-LSH lt is recommended to add a heat
filter
is built
in the
IMT2-LSH.
It is recom ende
to ad
a heat
filter
for
prolonged
observation
oror time-lapse
photography
of
filter
for
prol nged
observation
time-lapse
phot graphy
of tissue
tis ue
cultures, crc,
cult res,
II
etc.
6
PHOTOMICROGRAPHY
6-1 Photomicrographie System
Th
The
IMIMT.2
T-2
microsco micrse
e
features
provsior
features
provisons
s&cted accordino
to
oreference.
sel cted
ac ording
to pref rence.
,'1,tiri
mounti g
for
;:.l
of
oh tomicrographic
at cit
hments
cnrncnis at t 44 peces,
which
can
bbe
places,
which
can
A,
light
path
ffor
35mm
camera
back)
A. 0M
OM
light
path
(for
35m
camera
back)
A
35mm
camei'a
back
can
be
rnourued
in
same
way
aa camera
1) A 35 m
camera
back
can
be
mounted
in the
the
same
way
camera
lens
on
(Fia,
38)
tens
is is babayonet-rnojnîed
yonet-mounted
on aa camera,
camera.
(Fig.
38)
to
pictures
of
specimens
in
** It lt is is not
not recommended
recom ende
to take
take
pictures
of floating
floating
specimens
in
liquid
or
with
objectives
40X
or
to
liquid
or on
on aa micropipette.
micro-pi et ,
with
objectives
40X
or higher,
higher,
to
avoid
shutter
vibration
as
as
avoid
shuter
vibration
as much
much
as possible,
pos ible.
magnification
magnif.
xx 2.5
** Image
Image
magnifcation
= Objective
Objective
magnif.
2.5 xx Intermediate
Intermediate
magnif
(iX
or
magnif.
(IX
or i1.5X) .5X
Ex.
40x2.5x
== 150(X)
40
x 2.5
x L5X
1.5X
150
(X)
Ex.
Fig, 38
Focus
specimen,
through
the
binocular
tube,
Focus
onon ththe
e
specimen,
lo klooking
ing
through
the
binocular
tube.
2)
B.
light
path
8. MMTU
TU
light
path
direct
moUnting
of
attachment
PM-lOAD)
(Ifor
for
direct
mounti g
of the
the photomicrographic
phot micrographic
at chment
PM-IOAD)
Yöu
can
take
pictures
shutlet'
vibration,
since
the
attachment
You
can
take
pictures
wwithout
ithout
shuter
vibration,
since
the
at chment
IS
is desigdesigned
ned
vibrationvibration'proof.
-pro f.
1)
The
photo
eyepieces
available
NF
SX
and
6.7X.
The
phot
ey piec s
av ilable
incinclude
lude
NFK2.5X, K2.5X, 3.3X,
3. X,
5X
and6.7X.
** Insert
the
eyepiece
the
port
on
left
of
Insert
the photo
phot
ey piec
iinto
nto
the
port
on the
the
left side
side
of the
the
base,
base.
CEi9.
391
(Fig.
39)
** When
you
the
eyepiece
into
the
tube,
you
When
you insert
insert
the photo
phot
ey piec
into
the photo
phot
tube,
you
may
feel
resistance
midway
because
make
sure
to
may
fe l
resi tance
midway
because
of of a
a spring;
spring;
make
sure
to
completely
insert
the
eyepiece
until
it
stops.
complet ly
insert
the
ey piec
until
it stops.
2)
FHg. 39
3)
Mount
the
photornicrographic
attachment
40
Mount
the
phot micrographic
at chment
aas
s indindicated
icated
inin Fig.
Fig.
40.
lithe
unit
is
tilted,
the
Image
will
be
also
tilted
against
the
focusing
If the
unit
is tiled,
the
image
wil
be also
tiled
ag inst
the
focusing
reticle
in
microscope.
To
avoid
make
itit aa point
to
reticle
in the
the
microscope.
To
avoid
tiltilting,
ng,
make
point
tovisualy visually
parallelize
the
horizontal
cOntour
of
the
attachment
to
horipar leize
the
horizontal
contour
of
the
at chment
to the
the
horizontal
line
of
the
microscope.
zontal
line
of the
microscope.
Imane
magnification
Image
magnifcation
Ohjcctive
magri
f. x x Intermediate
xx NFK
= Objective
magnif.
Intermediate
magrnagnif,
nif.
(1X (iX oc
or 1.5X)
1.5X)
NFK
pnotc
eyepiece
magnif.
x
Camera
magnif,
(iX
for
35mm
or
phot
ey piec
magnif.
x Camera
magnif.
(1X
for
35 m
or
3X
for
format)
3X
for large
large
format)
Ex.
40x1.5x5xlX=300(X)
Ex.
40x
1.5x
1X=30 (X)
4)
Focusing
Focusing
Focus
rotating
the
helicoid
mount
of
Focus
thethe fraframe
me
reticlreticle
e
first, first, bby
y
rotaing
the
helicoid
mount
of the
the
left
hand
eyepiece
wbe.
fl'en
focus
the
left
hand
ey piec
sle ve slve of
of the
the binocular
binocular
tube,
then
focus
the
specimen
by
fine
focus
knoo.
specimen
by means
means
of of the
the
fine
focus
knob.
Fig
40
Fig.
40
E
C
C.
MTU
light
exit
C. MMounting
ounti g
the the mumulti-photo
lti-phot
tubtube
e
to to the
the
MTU
light
exit
Mount
multiphoto
tube
to
MTU
hgh
exit
port;
then
clamp
Mount
ththe
e
multi-phot
tube
to the
the
MTU
light
exit
port;
then
clamp
photomicrogniphic
411
ththe
e
phot micrographic
at cattachment
hment
on on it. it. (Fia
(Fig.
41)
PM-iOAD
system
isis used
in
way,
the
following
opt
IfIf the
the
PM-1OAD
sy tem
used
in this
this
way,
the
fol wing
options [ons are
are
aavailable.
v ilable:
exposure
measurement
PM-lOADS)
SSpot
pot
exposure
measurement
(wit(with
h
PM-lOADS)
l
viewer
PM-VSB
Bright rghl trame
frame
viewer
PM-VSB
16mm
cino-photomicrography
(PM.l6mm
Cinel,
which
enables
16 m
tine-phot micrography
(PM-16
m
Cine),
which
enables
easy
easy
film
loading
and
prevents
shutter
vibration.
film
loading
and
prev nts
shuter
vibration.
l
.
41
FFig.
ig.
41
the
photo
adapter
mounted
MILI
port
and
replace
itit
1li) Unscrew
Unscrew
the
phot
ad pter
mounted
oon
n
ththe
e
MTU
port
and
replace
the
adapter
CD
wwith
ith
the MTU
MTU
ad pter
0.
2)
2) Insert
Insert
tthe
he
MMTU
TU
rrelay
elay
tubelute @
(Fig.
41
(Fig.
41)
into
the
MTU
adapter
ff1
damp.
into
the
MTU
ad pter
@ and
and
clamp.
41)
((Fig.
Fig.
41)
'3)
the
bese
clamping
screw
and
place
the
on
3) Loosen
Lo sen
the
base
clamping
screw
08),
,
and
place
the baso
base
on the
the desk.
desk.
41)
((Fig
Fig.
41)
4)
Rotate
the
44 leveling
screws
there
in
with
the
Rotae
the
lev ling
screws
@ ® to
to keep
ke p
them
in contact
contact
with
the
desk
surface.
IFig,
41)
desk
surface.
(Fig.
41)
I
Fig 42
42
Fig.
J
I
5)
Clamp
41f
Clamp
the the base baseclamping clamping
knob@. lcriob'. (Fig.
(Fig.
41)
6)
Az'ach
tube
Atach
thethe tritrinoojiar
nocular
tube
0.
7)
Insert
the
photo
eyepiece
photo
tube.
Insert
the
phot
ey piec
intointo the
the
phot
tube.
8)
Corn-ïeci
AD aattathment
421
Con ect
the the PM-1PM-lo
OAD
t chment
@. t IFig.
(Fig.
42)
using
the
Unit
is is used,
out
Ir* When
When
using
the multitube
multi-ube
unit IMT-2MTU
IMT-2MTU
used,
out ofof focusfocusing
or
may
cause,
since
the
ophcal
path
length
isis long.
ing
or framing
framing
may
cause,
since
the
optical
path
length
long.
** Please
be
toto use
magnifier
Ii0 built-in
the
Please
be sure
sure
use the
the focusing
focusing
magnifer
built-n
the
photographic
attachment
for
or
phot graphic
at chment
for focusing
focusing
or framing.
framing.
(Fig.
42)
(Fig.
42)
J
rJ
D.
the
trinocular
tube
on
frame
D. Mounting
Mounti g
the
trinocular
tube
on the
the microscope
microscope
frame
Fig. 43
43
Fig.
In
of
In place
place
of standard
standard
binocubinocular
lar
tube, tube,a atrinocular trinocilar tubtube
e
BHBH2.TR
2-TR
@®can
can
be
and
used
with
the
DAD system
be attached
at ched
and
used
forfor photomicrography
phot micrography
with
the PM-i
PM-IOAD
sy tem
43)
0. , (Fig,
(Fig.
43)
** inIn case
photomicrography,
the
frame
viewer
case ofof fluorescence
fluorescence
phot micrography,
the bright
bright
frame
viewer
PM-VSR
(Fig.
PM-VSB
@'Ci"may maybe beused usedto tofaciltaefacilitate ffocusing.
ocusing.
(Fig.
43)
43)
the
path
selector
knob
to
BI
** Rotate
Rotae
the light
light
path
sel ctor
knob
to the
the
BI position,
positon.
tIr In In order
to
focus
on
the
specimen
looking
through
the
order
to focus
on the
specimen
lo king
through
the binocular
binocular
tube,
itit isis necessary
to
finder
eyepiece
tube,
nec s ary
to use
use aa suitable
suitable
finder
ey piec
0.
(Fig.
43)
(Fig.
43)
6-2 Taking Pâctures
rogcaphft eqprnent
forfor detailed
ptocedure,
This
RRefer
ef r
to to the
the
instructions stwctions provded
provide
with
eaeach
ch
phot micrographihotorn
c
equrpment
detailed
procedure.
This paraqranh
par graph
is
given
to
oxp1in
the
photomicrographic
is grven
to explain
the
phot micrographic
prproblems
oblems
pertaDertaining
in g
to tothe the IMT-2.lMT2.
.
0O Illumination
Ilumination
Accurate
illumination
is
important
for
than
Ac urate
ilumination
is more
more
important
for
phot micrograhotomic'ography
phy
than
observ
on since
flawle
pictures
ffor
or
observation
since
flawles
pictures
can ot cannotbe betaken taken
without whou it.
it.
In
to
uneven
especially
In order
order
to avoid
avoid
unev n
ilillumination.
umination,
especialy
withw'th hhigheontrast
igh
contrast
film,film,
the
iluminaticn.
foflowng
thethe observation
procedure
accuracy,
aadjust
djust
the
ilumination,
fol wing
observation
procedure
ac uracy.
Heat
from
the
Lght
Source
@O Radiant
Radiant
Heat
from
the
Light
Source
Even
optimum
intensity
ofof illumination
light
consderabln
Even
optimum
intensity
ilumination
light generates
gen rates
considerable
radtant
neat
for
oror photomcrography,
especially
inin case
radiant
heat
for observation
observation
phot micrography,
especialy
case
of
interference
contrast
Living
specimens
are
subject
to
of Nomarski
Nomarski
interf ence
contrast.
Livng
specimens
are
subject
to
damage
heat,
Therefore;
damage
due due to to radiant
radiant
heat.
Ther fore;
l
l
l
Peduce
light
intensity
as
as
Reduce
light
intensity
as much
much
as possibte.
pos ible.
Use
heat
filters,
Use
adadditional
itonal
heat
filters.
For
time-lapse
photography,
For
time-lapse
phot graphy,
sysynchronize
nchronize
the the lightlight source
source
to w e,
exposure
Isynch.
mode).
posure
(synch.
mode).
O
and
lamp
Voltage
0 Filters
Filters
and
Lamp
Voltage
Select
and
bulb
film:
Sel ct
thethe filtefilter
r
and
bulb
voltage ltao according
ac ording
to'o the
the
film
Film
Jit type
color
film
type
col r
film
Tungsten
type
color
film
Tungsten
type
col r
film
45-LBD-2N
45.LBD-2N
45
45-LBT LBT
B&Vi
B & W film
film
43.lF55O.W45
43-l
F5 0-W45
Da',
Daylrght
Voltage
F il ter
8v
8V
8V
8V
6V
uc
6V and
and
up
** ToTo match
your
inin color
rendition,
it it is is rer.ommended
to
match
your preference
pref rence
col r
renditon,
recom ende
to
make
exposure
determination
of
bulb
voltage.
make
tetest
st
exposure
forfor the
the
det rmination
of optimum
optimum
bulb
voltage.
S,
x
+
-
rig
44
Fig.
4
O
0 Focu5ing
Focusing
1)
\/
/\
2)
Looking
through
the
hnder
eyepiece,
adjust
diopter
ring
so
Lo king
through
the
finder
ey piec ,
adjust
tthe
he
diopter
ring
so
that
the
cross
lines
can
be
observed
as
distinctly
that
the double
double
cros
lines
can
be cleerly
clearly
observed
as twtwo
o
distnctly
(Fig.
44)
separate
lines.
separ te
lines.
(Fig.
4)
Bring
the
specmen
into
focus,
rotating
the
and
fine
adjust.
Bring
the
specimen
into
focus,
rotaing
the coarse
coarse
and
fine
adjustment
knobs
ment
knobs.
** Since
the
reticle
and
are
align
Since
the focusing
focusing
reticle
and she
the film
film plane
plane
are inin precise
precise
alignment,
the
focused
through
the
magnifier
and
the
ment,
the image
image
focused
through
the focusing
focusing
magnifer
and
the
image
in
are
aiat the
time.
Therefore,
image
in the
the film
film plane
plane
are inin focus
focus
the same
same
time.
Ther fore,
unless
adjustment
just
blurred
pictures
unles
tthe
he
adjustment
just described
described
iss perfect,
perfect,
blured
pictures
will
result.
wil
result.
** When
using
the
unii
is iused,
out
When
using
the multìtube
multi-ube
unit IMT2-MTU
IMT-2MTU
s used,
out ofof focusfocusing
or
may
cause,
since
the
path
length
isis long.
ing
or framing
framing
may
cause,
since
the optical
optical
path
length
long.
** Pleate
be
toto use
magnifier
the
Please
be sure
sure
use the
the focusing
focusing
magnifer
@ builtin
built-n
the
photographic
attachment
for
focusing
or
framing,
phot graphic
at chment
for
focusing
or framing.
:
20
O FramFrjn
ing
@
patti
ODM
M
lighIiht
t
path
A.
Frame
trarne
reticie,
Frame
thethe sspecrnen
pecimen
imaimage
ge
into into the the 3535mm
m
frame
reticle.
(Fig. Fig. 45)
45)
Fig.
45
Fig.
45
B.
MT1J
path
MTU
lightight
t
path
Frame
e'epieces
2.5X,
33X
and
Frame
rereticles
tiles
are are matcimatched
ed
to to NFKNFK photo
phot
ey piec s
2.5X.
3. X
and
in
order
from
outer
to
The
outside
ret
trame
5SX
X
in this
this
order
from
outer
to inner.
in er.
The
outside
reticle ide atat each
each
frame
indicates
and
indicates
35m 35mm film film format
format
and
thethe ininside
side
reticle reticleindicates ind,caes4”~ 4'x 3” 3'
foformat,
rmat.
Picture
the
trame
Picture
aarea
rea
on on the the film film plane
plane
wwithin
ithn
the
frame
Format
Format
4”
x 3”
Pictueaea
Picture
area
frame
35
35 mm
m
21.5mm
xx 32,5mm
21.5
m
32.5
m
44” xx 3"
3”
65
65m x85m miii x
j mm
Fig,
46
Fig.
46
O
Shutter
@ Eyepiece
Eyepiec
Shuter
I
When
remove
your
tace
from
the
tube
while
the
When
yo'ou
u
remove
your
face
from
the binocular
binocular
tube
while
the camera
camera
sshutter
huter
isis oopen
pen
for fr
a a long
time,
room
light
may
enter
the
long
time,
ro m
light
may
enter
the eyepeces,
ey piec s,
forming
an
image
coincident
n
th
specimen
image
forming
an
image
coincident
on
the
specimen
image
toto be
be phOto
phot -
graphed.
To
this
light,
pull
sht.iF
ter
graphed.
To exclude
exclude
this extraneous
extraneous
light,
pul out
out thethe eyepiece
ey piec
shuter
time
of
{Fig.
47)
aat
t the
the
time
of exposure.
exposure.
(Fig.
47)
L
t:
D
)
)
)
Fig.
47
Fig.
47
7
TROUBLESHOOTING GUIDE
are
uriabe
to
opIfIf ynu
you
are
unable
to obta.n
obtain
optimum
shooting.
trtrouble
ouble
sho ting.
-i.m perforrnwce
from
your
microscope,
please
the
table
below
fer
perfomance
from
your
microscope,
please
concnsuIt
sult
the
table
below
for
Trouble
Cause
IRemedy
system
1.1. Optical
Optical
sy tem
a) 31SwitchingSwitchingon on lightIght source.
source,
fieldfi&d
is
oof
f view
view
is si
stil ill dnrk,
dark.
cord
is
connected
Bulb ulh socket
socket
cord
is not
not
con ected.
Connect
cord
to
Ori
Con ect
ththe
e
cord
to receptacc
rec ptacle
on
the
microscope
stand.
the
microscope
stand.
Bulb
is
out.
Bulb
is burned
burned
out.
Replace.
Replace.
Sliding
voltage
control
ever
isis set
Slidng
voltage
control
lev r
set at
at
low
aa position,
ttoo
o0
low
positon.
Set
itit at
voltage
Set
at higher
higher
voltage
pposition.
ositon.
Bulb
¡s
centered.
Bulb
is nnot
ot
center d.
Center
it.
Center
it.
Condenser
Condenser
holderholder is is notnot claclanped
mped
in
n
Clamp
it,
Clamp
it.
place.
place.
Condenser
Condenser
is is not not inin corcorrect
ect
pposition.
ositon.
Adjust
condenser
height
until
field
Adjust
condenser
height
until
field
diaphragm
image
is
inin specidiaphragm
image
is formed
formed
specimen
piane.
men
plane.
Condenser
is
centered,
Condenser
is not
not
center d.
Nosepiece
in
Nosepiec
is is notnot clicked
cli ked
in place.
place.
Center
itit until
field
diaphragm
Center
until
field
diaphragm
image
comes
in
center.
image
comes
in the
the
center.
Rotate
itit clicks
inin
Rotae
it¡t slightly
slightly
ununtil
til
cli ks
placo.
place.
Light
path
selector
knob
is
Light
path
sel ctor
knob
is at
at the
the
0M
or
MTU
position.
OM
or MTU
positon.
Set
knob
BI
or
Set
knob
toto the
the
BI position
positon
or ir
in-
Too
nr
are
To
many any fìl-ters
filters
are engaged
enga ed.
Reduce
Reduce
nunumber
mber
ofof filters.
filters.
Stage
path.
Stage
insert insert plate plate blocks blcks light
light
path.
Remove
stage
and
reset
specimen.
Remove
stage
and
res t
specimen.
Fuse
Fuse
is is burneburned
d
outout.
.
Check
and
after
re
Check
eelectric
l ctric
cicircuit
r uit
and
after
re-
crease
bulb
voltage
crease
bulb
voltage.
moval
of
replace
moval
of cause,
cause,
replace
fulute,
se.
Line
voltage
selector
switch
is
Line
voltage
sel ctor
switch
is not
not
Adjust
switch
correctly.
Adjust
switch
corectly.
set
to
with
local
mains
set
to conform
conform
with
local
mains
voltage.
voltage.
bh)
Field
is
off
at
Field
oof
f view
view
is cut
cut
of
at the
the
periphery
or
periphery
or ililuminatedlurninated unevenunev nly.
IY.
path
selector
knob
isis stopped
LLight
ight
path
sel ctor
knob
stop ed
midway.
midway.
Click
knob
into
piece
eccordirig
in
Click
knob
into
place
ac ording
to
Nosepiece
magnification
Nosepiec
anand
d
magnifcation
changer
correctchanger
aae
re riot
not positioned
positoned
corect-
Click
there
into
oLc.e,
Click
them
into
place.
purpose.
purpose.
ly.
ly.
Condenser
turret
is
correctly
Condenser
turet
is not
not
corectly
positioned,
positoned.
is
is not
not
centered
correctly.
center d
corectly.
Condenser
Condenser
positioned
cr
positoned
or
Click
turret
into
place.
Click
turet
into
place.
Click
condenser
turret
into
place
or
Click
condenser
turet
Into
place
or
center
correctly
center
corectly.
L
C
C
c)
or
isis visible
in
01
c) Dust
Dust
or dirt
dirt
visble
in field
field
of
vieview.
w.
Light
source
is
centered.
Light
source
is not
not
center d.
Center
light
source.
Center
light
source.
I-
Filter
isis stopped
midway.
Filter
stop ed
midway.
Pull
or
itit completely,
Pul
or pusJ
push
complet ly.
C,
Dirty
specimen.
Dirty
specimen.
Clean
side
or
vessel,
Clean
slide
or culture
cult re
ves el.
C
Dust
on
Dust
on eyepiece,
ey piec .
Clean
eyepiece.
Clean
ey piec .
Condenser
is
correctly
POSiCondenser
is not
not
corectly
posiiior-eJ
and
the
frosted
filter
isis iOin
tioned
and
the
frosted
filter
fOcus.
focus.
Adjust
condenser
height
unfit
1iet
Adjust
condenser
height
until
field
diaphragm
on
sdiaphragm
image
mage
iis
s foformed
rmed
on
specimen
plane.
cimen
plane.
rr
C
C'
C
C-
C-
r
jroub4e.-.
d)
1) EEx:siveim
xces ive
image
Cause
Remedy
Condenser
Condenser
is isstop ed stnprio in
in high i ir posi- I::L,I
contrast. ontrast.
L.,'rr:::iinser
Lower
condenser.
tion
tion.
Aperture
Apertue
dcflaphragrn
iaphragm
down
excessively.
down
exces ively.
e)
problems:
?) Resolution
Resolution
problems:
l
ImImage
age
is is not flot sharp.
sharp.
0
contrast,
InInsufficient
suficent
contrast.
l
Objective
Objective
is
is
is
is
slopped
stop ed
not
correctly
posi
not
corectly
posi-
Open
diaphragm.
Open
diaphragm.
Clkk
nosepiece
into
place.
Click
nosepiec
into
place.
tioned
in
path.
tioned
in light
light
path.
Image
details
definition,
Image
details
llack
ack
defintion.
Aperture
Apertue
diaphragm
diaphragm
is
is
stopped
stop ed
down
excessively
too
down
exces ively
or or opened
open d
to
Adjust
aperluro
iris
opening
Adjust
apertue
irs
openi g
pro- rp.
perly.
perly.
mu
much. ch.
Correction
collar
is
adjusted
Corection
colar
is not
not
adjusted
correctly.
corectly.
Looking
at
image,
rotate
Lo king
at specimen
specimen
image,
rotae
correction
collar
until
optii'riurt
corection
colar
until
optimum
focusing
position
can
be
focusing
positon
can
be found.
found.
Dust
on
objective,
eyeDust
on condenser,
condenser,
objective,
ey -
Clean.
Clean.
piece,
vessel.
piec ,
cculture
ult re
ves el,
eetc.
tc.
f)‘) No
phase
contrast
No effective
ef ctive
phase
contrast
is s
obtaned.
obtained.
Thickness
Thicknes
of of vvessel
es el
bot m botwrn is
is more
more
than
than
22mm.
m.
Use
aa vessel
of
thickness
Use
ves el
of bottom
bot m
thicknes
obective
isis used.
BrBright
ight
fieficld
ld
objective
used.
Use
phase
Use
phase
obobjective,
jective.
Light
w'nulus
is
matched
with
Light
an ul s
is not
not
matched
with
Match
light
annulus
to
Match
light
an ul s
to objective.
objective.
less
les
thanthan 22mm.
m.
objective.
objective.
9)
out
g) Specimen
Specimen
imimage
age
is is parpartially
tialy
out
of
of focus,
focus.
Light
annulus
and
anhiUlUS
Light
an ul s
and phase
phase
an ul s
are
not
centered.
are
not
center d.
Center
them
correctly.
Center
them
corectly.
Objective
correctly
in
Objective
is s not
not
corectly
in light
light
path.
path.
Slighily
rotate
nosepiece
until
Slightly
rotae
nosepiec
until
itit
place.
cli ks likt into
into
place.
Specrnien
correctly
place
on
Specimen
is is nonot
t
corectly
place
on
Place
Place
spspec'merr
ecimen
corectcorrectly.
ly.
stage.
stage.
hI
h) Image
Image
iisblurred.
s blured.
Vessel
Ves el
bot m bottom isis not not flaflat.
t.
Use
bottomed
vessel.
Use
anan eevenly-flat
v nly-flat
bot med
ves el.
Condenser
centered
Condenser
is is not not cocorrectly
rectly
center d.
Center
condenser,
Center
condenser.
Light
source
correctly
cenLight
source
is s not
not
corectly
centetered
r d.
Center
light
source
Center
light
source
ccorrecily.
orectly,
Condenser
Condenser
holderholder isis tiled tilted up,
up.
Lower
itit to
position,
Lower
to stop
stop
positon.
Minimum
line
voltage
adjusirnern
Min mum
line
voltage
adjustment
Adjust
the
knob
until
bulb
isis dirnl
Adjust
the
knob
until
bulb
dimly
lit
with
er
lever
sliding
control
lit
with
slidng
control
lev r
at
2.
adjustment
2. Electrica'
Electrical
adjustment
J
a)
source
is
even
at
a) Light
Light
source
is totoo bright
bright
ev n
at
lowest
bulb
voltage.
lowest
bulb
voltage.
knot)
adjusted.
knob
isis notflot correctl',
corectly
adjusted.
lowest
position
lnearest
to
lowest
positon
(nearest
to yo'.jI
you).
D
D
3
b)
flickers
and
intensity
isis un
b) Lght
Light
flickers
and
intensity
unstable
stable.
Line
voltage
is
Line
voltage
is unstable.
unstable.
Bulb
to
out
bulb.
Bulb
filafilament
ment
isis likellikely
y
to burri
burn
out.
, Replace
Replace
bulb.
Loose
Lo se
el celectrical
trical
coconnection.
n ection.
)
D
rJ)
c)
burns
Out
often.
c) Fuse
Fuse
burns
out
ttoo
o
often.
Fjse
¡sis not
one.
Fuse
not aa standard
standard
one.
Bulb
isis not
a standard
one,
nt
stn rinri
RI Ilh
r
I
connections.
i Tighten
Tighten
con ections.
standard
fuse.
1 Use
Use
standard
fuse.
8
rlain ot rl
hzln on
hi,lh
i Use
I ICD a halogen
bulb
as designarec
112V EOW HAL).
-J
D
Use
Use
vvoltage
oltage
sstabilizer.
tabilzer.
c)
lamp lights up but halogen
d) Pilot
Pilot
bulb
does not when por switch
bulb
is
on.
is tuturned
r
Halogen bulb is burned oui.
Replace bulb,
No bulb is in socket.
Insert bulb.
Loose electric connections.
Tighten connections.
Trouble
CaUse
-
Remedy
focus
adjustments
33.
. Coarse
Coarse
anand
d
fintine
e
focus
adjustments
aal
) Coarse
Coarse
adjusadjustment
tment
is is to too tight.
tight.
b}
drops
and
specimen
goes
b) Stage
Stage
drops
and
specimen
goes
out
of
out
of focus
focus.
Tenson
adjustment
ring
istLghened
Tension
adjustment
ring
is tighten d
totco murh
much.
Tension
Tension
loose.
lo se.
adj
adjustment ustmern
ring
ring
is
is
too
to
Loosen
Lo sen
tension
tension
adustment
riny
adjustment
ring
properly.
properly.
Tighten
ring
properly.
Tighten
ring
properly.
4,
4. Observation
Observation
tutube
be
di
binocular
vision.
a) lnornplte
Incomplet
binocular
vison.
lnterpupillary
distance
is
corlnterpu ilary
distance
is not
not
cor-
Correct
interpupillary
distance.
Corect
interpu ilary
distance.
rectly
adjusted.
rectly
adjusted.
Diopter
adjustment
Is
Diopter
adjustment
is incomIete.
incomplet .
Complete
adjustment
Complet
diodioper
pter
adjustment.
Usor
is
User
is unaccustomed
unac ustomed
tto
o binocular
binocular
Pr1or
details,
Prio
to io lo klooking
ing
at ei specpecirren
imen
details,
vison.
iry
to
aiat the
entire
field
oof
try
to rook
lo k
the
entire
field
view,
or
atat aa far
obfec
view,
or look
lo k
far away
away
object
beorc
resuming
observation.
before
resuming
observation.
55.
. Stage
Stage
a) i Imege
Image
easieasily
ly
gogoes
es
out oui ot
of focus
focus
Stage
Stage
is is not not clampclmped,
ed.
Clarìp
stage
securely.
Clamp
stage
securely.
Specimen
is
pOStionOrI
Specimen
is nonot
t
ccorrectly
orectly
positoned
on
on stage.
stage.
Adjust
specimen
pO5itiofled.
Adjust
specimen
positoned.
Objective
is
so
Objective
is protruding
protuding
so as
as to to hit
hit
anst
stage
insert
plate,
ag inst
stage
insert
plate.
Lower
nosepiece
rotate,
Lower
nosepiec
anand
d
ththen
en
rotae.
when
you
touch
stage
when
you
touch
stage.
bh)
) Specimen
Specimen
stostops
ps
midmidway
way
onon the
the
west tratraverse,
eeast
ast-west
verse.
OLYMPUS
OLYMPUS
OPTICAL
CO.
LTD.
OLYMPUS
OPTICAL
CO.,
LTD.
BuIIdng,
Nshì
Shinuku
1-thome,
Shinjuku-4iu,
'Tokyo,
SSan-Ei
an-Ei
Buildng,
2 22.2.
-2,
Nishi
Shinjuk
1-chome,
Shinjuk u,
Tokyo,
JapJapan
n
CO.
(EUROPA)
GMBH.
OOLYMPUS
LYMRUS
OOPTICAL
PTlCAL
CO.,
(EUROPA)
GMBH.
Posfl¶ C4O8,
Werrns1rasse
14-16,
Postfach
104908,
Wendenstras e
14-16,
20 2000Hamburg Hanirg i 1, Germany
Germany
OOLYMPUS
LYMPUS
COCORPORATION
RPORATION
44 Nevada
Lake
N.Y.
r1 042-l 1C42-fl79,
U.5A.
Nevad
DriDive,
ve,
Lake
SucSuccas,
es ,
N.Y
179,
U.SA.
OLYMPUS
OPTICAL
CO.
(U.K.)
LTD.
OLYMPUS
OPTICAL
CO.
(U.K)
LTD.
Siroel,
Lonøon
228
-8 Honduras
Honduras
Stre t,
London
EClYOEC1.YOIX,
TX,
United Uned KinKinoorn
gdom
The
predut
t IS uflOe'
conterit
refiw
ond
The
desigdeiçn
n
of o the
the
product
under
constant
review
and
J
htst ev ry 'ìery efort Uort sis
ro
upup o to dare,
the
itIS rPsertet$
made
to keep
ke p
thisthis mamuaI
nual
date,
the rigPU
rtght
res rved
to to chacharqe
nge
speciflca- soecfalions
8fld
w1lou1
orior
rp.tCC
tions
and
equipeQt)ment
ment
at t any r.y limB
time
wthout
prio
notice
whilst
Printed
in
J~DX
901
M
04