PLAN DE RECHERCHE ET CALENDRIER
Transcription
PLAN DE RECHERCHE ET CALENDRIER
&+2& &+LOH3HUX2[\JHQ0LQLPXP DQG&LUFXODWLRQ RFWREUH :KHQ,FDPHEDFNRQERDUG,QHDUO\VXIIRFDWHGZLWK WKHFDUERQLFDFLGWKDWZDVVDWXUDWLQJWKHDLU+D:K\GLGQRW ZH KDYH WKH FKHPLFDO PHDQV WKDW ZRXOG KDYH DOORZHG XV WR GULYH DZD\ WKLV SRLVRQRXV JDV 2[\JHQ GLG QRW ODFN $OO WKLV ZDWHU LQFOXGHG LW LQ D FRQVLGHUDEOH TXDQWLW\ DQG LWV GHFRPSRVLQJZLWKRXUSRZHUIXOEDWWHULHVZRXOGKDYHUHVWRUHG WKHYLWDOIOXLG,KDGWKRXJKWDERXWLWEXWVRDVFDUERQLFDFLG SURGXFHG E\ RXU EUHDWKLQJ KDG LQYDGHG DOO WKH SDUWV RI WKH VKLS 7R DEVRUE LW FRQWDLQHUV VKRXOG KDYH EHHQ ILOOHG XS ZLWK FDXVWLF SRWDVK DQG EHHQ VKDFNHG SHUPDQHQWO\ %XW WKLV PDWHULDO ZDV ODFNLQJ RQ ERDUG DQG QRWKLQJ HOVH FRXOG KDYH UHSODFHGLW-XOHV9HUQH7ZHQW\7KRXVDQG0LOHVXQGHU WKH6HD PROGRAMME NATIONAL "PROcessus biogéochimiques dans l'Océan et Flux" )RUPXODLUHGpWDLOOp THEME (S) CONCERNÉ(S) :Thèmes 1, 2 et 3 RESPONSABLES SCIENTIFIQUES DU PROJET : (1) Diana Ruiz-Pino , et Maître de conférences Paris VI. [email protected] (2) Yves du Penhoat , Directeur de recherches IRD. [email protected] (2) Coordination : Gérard Eldin , Chargé de recherches IRD Gé[email protected] LABORATOIRES DES PROPOSANTS : (1) : Laboratoire de Biogéochimie et Chimie Marines (LBCM), UMR CNRS 7794 4 Place Jussieu, Boite 134 75005 Paris Directeur : Alain Poisson. (2) : Laboratoire d’Etudes en Géophysique et Océanographie Spatiales (LEGOS), UMR CNRS 5566 14 Avenue Edouard Belin 31400 Toulouse. Directeur : Christian Le Provost. Titre du projet : CHOC (Chili-Peru : Oxygen Minimum et Circulation) Résumé du projet et Budget 2003 : voir ci-après Durée du contrat demandé : 4 ans Montant demandé cette année au progrmmes nationaux: 49.06 k• Projet nouveau Visa obligatoire du Directeur de formation Signature du demandeur : 7DEOHRI&RQWHQWV $EVWUDFW 'HWDLOHGEXGJHWIRU 6FLHQWLILF5DWLRQDOH 0RWLYDWLRQ 7KH6RXWK(DVWHUQ3DFLILF20= 1.2.1 The regional oceanic circulation %LRJHRFKHPLFDOFKDUDFWHULVWLFVRIWKH6RXWK(DVWHUQ3DFLILF 3DOHRFHDQRJUDSK\RQWKH3HUX&KLOHPDUJLQ 7KHREMHFWLYHVRI&+2& 2FHDQLFSK\VLFVLQWKH20=RIWKH6(3 %LRJHRFKHPLVWU\RIWKH20= 1.3.3. 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contrat global IRD ) 3,00 k• (LODYC) constitution base sédimento avec IMARPE) PUBLICATIONS 12 k• (envoi containers France –Chili et France-Perou) TRANSPORT EQUIPEMENT ÉCHANTILLONS 3 k• envoi échantillons MAINTENANCE EQUIPEMENT 4,20 k• (adaptation bathy 200 et transducteur Khz pour navires étrangers chiliens et péruviens) 8 k• (Au Chili) 8,00 k• (Au Chili) FRAIS DU TERRAIN SUB-TOTAL 17.6 k• 15 k• 19,2 k• (48,3(0(17 68%727$/ 0,66,216 352*5$00(6 1$7,21$8; &$03$*1(6 92<$*(6 68%727$/ 727$/ N¼,36/ GHPDQGp N¼830&XULH GHMDDFTXLV 10.9 k• manip O2 faibles concentrations 3k• : PC portable + imprimante (automatisation manip oxygène) N¼ k• &KLOLHQV k• )UDQoDLV 5(81,216 SUpSDUDWRLUHVjODFDPSDJQH k• 'DQRLV N¼86$ GH N¼8. 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able 2. Goals and description of experiments performed during the fixed stations by the french biogeochemistry team 0DLQ*RDOV Specific goals Impact of the POM remineralization in the OMZ intensification Impact of the biogeochemical processes in the OMZ intensification Nitrogen assimilation and transformation in the microbial community Impact of the OMZ in the biogeochemical processes associated with the POM (C, N) and CO2/ N2O fluxes Nitrate and ammonium uptake Nitratoreduction Nitrification Denitrification POM regeneration and nitrogen production Transformation of POM and nitrogen remineralization Generation of DOC, DOM, DOM (oxic-anoxic conditions) Experiments description Short incubations (6-24 h) POM enrichment experiments with waters associated with different oxygen conditions Number of experiments (each fixed station) Measurements Oxygen, CO2, DOC, DON, alkalinity, salinity. 4 levels (oxicline, OMZ, upper and lower boundaries layers Ammonium, nitrato, Bacterial biomass and identification Pigments 1LWURJHQVWDEOHLVRWRSHV Short incubations of OMZ and deep waters with -tracers 15N-NO3-, 15N-NH4 , 15N-N2 6 levels -blockage acetylene Long incubations (3 days) of surface waters with tracers 13C et 15N-NO3- One experiments/ by station POM enrichment experiments with waters with different oxygen conditions 4 levels (oxicline, OMZ, upper and lower boundaries layers Quality (lability) of the POM quantificated by tracers Quality of the POM $PPRQLXPQLWULWH QLWUDWH )RVIDWH QLWURJHQPROHFXODLUH 1LWURXVR[LGH Isotope signature in the particulate fraction of phytoplankton (>0.6 PM), and bacteria (<0.6 PM) and in the dissolved fraction (DON, ammonium, nitrate). MOP, and DOM, POC, NOP Technics/ analyses Winkler optimized for low oxygen. CO2 by potentiometry titration. Colorimetry (Tréguer and Le Corre, 1975) Clasical and Molecular approaches 5HVHDUFK WHDPV COM (Raimbault) LBCM (team CO2 and Organic ) (1) LOV (Marty) Mass spectometry Colorimetry (Tréguer and Le Corre, 1975) COM (Raimbault) COM (Bonin) LBCM (CO2 team) (1) Gas chromatography Mass spectrometry Method semiautomatique (Raimbault et al., 1999ª y b) Analyses nutrients by auto-analyser. Ammonium, nitrato, nitrogen moleculaire Gas chromatography Nitrous oxide CPG, CPG/SM, CPG/AED. COM (Raimbault) LBCM (organic team and CO2) Oxydation of fatty acids mono-insaturated DIC (carbonate, CO2), PCO2, pH, alkalinity Uptake of different DIC forms Incubations en controled oxygen conditions 4 experiments per day 13 C different fraction size of phytoplankton Pigments CO2 by potentiometry titration. Mass spectometer HPLC LBCM (team CO2) LOV (Marty) (1) Oxygen, DIC, alkalinity, pH POM enrichment experiments with waters with different oxygen conditions CO2 and N2O coupling Incubations dans conditions controlee de lumiere et oxygene -with isotopes -inhibiteur acetylene 4 levels (oxicline, OMZ, upper and lower boundaries layers oxide nitrous. Molecular nitrogen Nitrate, nitrite, ammonium Isotopes 15N Identification et activity of microbial communities Winkler optimized for low oxygen. CO2 by potentiometry titration. 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(in-situ pump) matter Fixed Free-drifting Sediments (1) (2) T-S T-S currents Altimetry, SST, Seawifs TCO2-O2 PCO2 Bacterial identification & activity, associated to N, C cycles, bacterial mats N2O, CO2 TCO2-O2, PCO2 TCO2-O2, PCO2 Alkenones, SST, hydrocarbons, diols, alcohols, sterols, fatty acids Alkenones, SST, hydrocarbons, diols, alcohols, sterols, fatty acids Alkenones, SST, hydrocarbons, alcohols, diols, sterols, fatty acids (2) Lipids,Carbohydrates Proteins (NMR) Lipids,Carbohydrates, Proteins (NMR) Lipids,Carbohydrates, Proteins (NMR)(2) 13 C & 14C of OM, N of OM, detritus U, Th, Pa, 15N, 13C Palynofacies OM 15 13 18 18 13 Palynofacies OM (1) O2, S 13 C DIC C & O foraminifera O & C foraminifera TEP C,N,P Palynofacies OM TEP C,N,P (1) - - NO3 , NO2 + TEP Chlorophyl-a (fluorometry) Alkenones, SST, hydrocarbons, alcohols, diols, sterols, fatty acids Name Y. Du Penhoat B. Dewitte G. Eldin A. Vega D. Ruiz-Pino A. Poisson M. Graco A. Paulmier B. Schauer J. Guigand I.Bouloubassi L. Méjanelle Lipids,Carbohydrates, Proteins A. Gogou (NMR) J. Fillaux A. Lorre Sedimentology, Seismic L. Ortlieb particles 13 C & 14C OM, sedimentology G. Vargas 15 N of OM 15 13 U, Th, Pa, N, C L. Dezileau Palynofacies OM A. Sifeddine Geochem. bulk & traces F. Lecornec Minerology and X-Ray S. Caquineau V. Echevin 13 18 C & O of foraminifera C. Pierre Geochem. traces for P. Soler foraminifers 18 13 O & C foraminifera J.F. Saliège TEP X. Mari N. Garcia (1) + NH4 , PO4 C,N & P particulate DOC, DON, DOP 15 15 13 (1) NO3, N2, C, 15N 15 (1) (PON) NH4 (1) C,N,P, POC, PON, POP P. Raimbault C. Fernandez F. Diaz Laboratory LEGOS/GRGS Toulouse UMR-CNRS 5566 LBCM, Paris UMR 7094 UPMC-CNRS LBCM, Paris UMR 7094 UPMC-CNRS IRD, Bondy Equipe Paléotropique UR 055 LODyC, Paris UMR 7617 UPMC-IRD-CNRS COM, Marseille UMR 6539 CNRS-Univ. Aix- Marseille II Country )5$1&( N cycle of bacteria (1) (molecular biology) 234 BSi Th; Po; Mass flux ; POC; PIC; PON; PIN ; Chl-a 210 234Th Th; Po; POC; PIC; (2) PON; PIN ; Chl-a 234 (2) Th 230 BSi Si (OH)4 BSi, production Si, Ks, (1) Vmax, Si dissolution %BSi, Si(OH)4, Si dissolution Pigments Pigments Pigments (HPLC) / (1) Primary production (total); 210 Po (total) 234 210 BSi, Isotopes Si 210 137 238 Th ; Pb ; Cs ; Pu ; 239,240 Pu ; TOC ; TIC Bsi, Isotopes Si Siliceous species production 15 13 Diatoms ( N & COrg.) Nanoplankton & coccolithophorides Planktonic foraminifera Dinoflagellates 15 P. Bonin 13 TOC, TIC, N & C Org. Benthic foraminifera 13 (species, C), Mn/Ca Siliceous species production V. Michotey J.-C. Miquel B. Gasser A. Leynaert O. Ragueneau J.-C. Marty J. Chiavérini J.-J. Pichon LOB-COM, Marseille UMR 6535 IAEA, Monaco Marine Environment Laboratory LEMAR, Brest UMR-CNRS 6539 LOV, Villefranche/s/Mer Diatoms ( N & C Org.) X. Crosta EPOC, Bordeaux UMR 5805 Univ. Bordeaux- Nanoplancton and coccolithophorides Planktonic foraminifera J. Giraudeau CNRS Dinoflagellates 210 Pb (surface sediement) 234 Th (>>) 15 13 TOC, TIC, N & C Org F. Eynaud J.M. Jouanneau Benthic foraminifera (species13 C), Mn/Ca F. Jorissen J.P. Debenay E. Geslin S. Ernst F. Redois C. Griveaud 15 13 C. Pujol P. Martinez Laboratoire d'Etude des Bio-Indicateurs Actuels et Fossiles Université d'Angers CNRS UPRES EA 2644 )5$1&( N20, PN2O, NH4, O2 (low conc.) Pigments ; PP; flow cytometry; picophytoplankton, picoeucaryotes, nano- to micro-plankton, microbial identification and biomass (ATP) Nitrification & denitrificationmetabolism of the microbial community (ass. To the N cycle) O. Ulloa L. Farías Currents, Physical processes O2 O. Pizarro V. Villagran G. Alarcón Pigments, primary production N2O-O2-nutrients Physical processes, modelling Physical processes, modeling C. Rivera S. Hormazabal N, 13C 15 N, 13C N2O/ production N2O Physical processes Bacterial identification (Nitrification and denitrification) Minerology, heavy mettals, contaminants Foraminifera Foraminifera Excretion of NH4+ and other nitrogencompounds Alkenones, SST, pigments, chlorines 15Ncompound specific Siliceous phytoplankton Alkenones, SST, pigments, chlorines 15 N-compound specific &+,/( O. Leth 15 Phytoplankton and zooplankton (biomass) COPAS Concepcion, Chile University of Concepcion C and N in phytoplankton and zooplankton. R. De Pol M. Castro M. Ramos V. Molina V. Pineda M. Marchant C. Morales Alkenones, SST, pigments, chlorines 15N-compound specific S. Pantoja Siliceous phytoplankton C. Lange Departement of Zoology Faculty of Natural Sciences and Oceanography University of Concepcion Dep. of OceanographyCOPAS Concepcion Faculty of Natural Sciences and Oceanograpgy University of Concepcion Fecal pellets/ Agreggates, C, N, P Palynofacies, OM Diatoms (species) Benthic foraminifera Structure of planktonic community Fecal pellets/ Agreggates, C, N, P H.E. Gonzalez Institute of Marine Biology University Austral Palynofacies, OM Diatoms (species) Benthic foraminifera J. Valdés L. Rodriguez M. Paez Facultad de Recursos del Mar Antofagasta University of Antofagasta Metals (speciation, Cu, Fe, Mn, Co) Phytoplankton (molecular biology) D.A. Hutchins Metals (speciation, Cu, Fe, Mn, Co) J. Moffett University of Delaware, Newmark, USA Departement of Civil and Environnemental Engineering and Biology Department MIT, Cambridge, MA, USA WHOI, MC&G, Woods Hole, MA, USA WHOI, MC&G, Woods Hole, MA, USA DCESS, Copenhagen, University of Copenhagen, Denmark Organic Geochemistry Unit, Organic and Biological Chemistry Section, Bristol, UK OM composition (NMR) Fecal pellets /Aggregates C, N, P Palynofacies, OM Diatoms (species) Benthic foraminifera S.W. Chisholm OM composition (NMR) OM composition (NMR) TRAMP, yo-yo, FluoO2-T-S G.R. Shaffer 13 13 C-compound specific C-compound specific T-S Chemical characterization of TEP D. Repeta Bioturbation in surface sediment Chemical characterization of TEP 13 C-compound specific R. D. Pancost D. Gutierrez Chemical characterization of TEP P.H. Santschi IMARPE, Lima, Peru Univ. of Texas A&M, Galveston, TX, USA %,%/,2*5$3+<)256(&7,216$1' $KXPDGD 5 $ 5XGROSK 1 6LOYD 6XU &LHQF 7HF 0DU $OWDEHW 0 )UDQFRLV 5 *ORE %LRJHRFKHP &\FOHV $OWDEHW0$)UDQoRLV50XUUD\':HW3UHOO:/ 1DWXUH Altabet M., Pilkaln C., Thunell R., Pride C., Sigman D., Chavez F., Francois R. (1999) Deep Sea Res., 46, 655-679 Altabet, M.A., Higginson, M.J. and D.W. Murray (2002) Nature (415), 159-162 Andersen, J.J., Okubo, A, Robbins, A.S. and A.F. Richards (1982): Deep-Sea Res., 29, 1113-1140. Archer, D., A. Winguth, et al. (2000). 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