DIAION® and SEPABEADS®

DIAION® and SEPABEADS®
Synthetic Adsorbents
RESINDION SRL
Via Roma, 55 - 20082 Binasco (MI) - ITALY
Phone: +39.02.900130.1 - Fax: +39.02.90090774 - E-mail: [email protected]
What are Synthetic Adsorbents?
l
Spherical crosslinked polymer particle with porous structure
l
No ion-exchange groups or functional groups
l
Adsorb organic compounds on the surface due to hydrophobic
interaction
l
Highly porous structure
Pore diameter :
several tens – several hundreds angstrom
n High adsorption capacity
n Selective adsorption utilizing size exclusion effect
n
DIAION ® HP20
Chemical Structure of Synthetic Adsorbents
l
Polystyrenic Adsorbents – Standard type
DIAION ®
n SEPABEADS ®
n
n
l
MCI ® GEL
HP20, HP20SS, HP21
SP70, SP700, SP825, SP850
CHP20A, Y, P, CHP55A, Y
Chemically Modified Polystyrenic Adsorbent
n
SEPABEADS ®
Br
Br
SP207
Br
Br
Higher adsorption capacity
High gravity (1.2)
– Applicable to Expanded Bed Adsorption
or Countercurrent operation
Br
Br
CH3
C O CH3
OCH3
l
Polymethacrylic Adsorbent
®
DIAION
n MCI ® GEL
n
O C
CH3
O
HP2MG
CHP2MG, CHP2MGY
More polar than polystyrenic resins
Applicable to normal-phase adsorption
C O
OCH3
CH2
CH2
O
O C
CH3
OCH3
CH3
C O
C O CH3
OCH3
What Kind of Compounds can be Separated?
l
Antibiotics from Fermentation Broth
l
Herbal Extracts
l
Peptides and Proteins
l
Vitamins
List of Industrial Synthetic Adsorbents
Product Name
SP850
SP825
SP70
SP700
HP21
HP20
SP207
HP2MG
Water content
46-52%
52-62%
55-65%
60-70%
45-55%
55-65%
43-53%
55-65%
Particle size
on 250µm
90% min.
-
-
90% min.
90% min.
on 300µm
Effective size
(mm)
0.25 min.
-
-
0.25 min.
0.35 min.
Uniformity
coefficient
1.6 max.
-
-
1.6 max.
1.6 max.
Specific surface
area
(m2/g)
1000
1000
800
1200
570
600
630
470
Pore volume
(ml/g)
1.2
1.4
1.6
2.3
1.1
1.3
1.3
1.2
Pore radius
(Angstrom)
38
57
70
90
80
260
105
170
Cephalosporin C
Adsorption (g/L)
85
76
60
76
48
38
119
<10
Pore Size Distribution of Synthetic Adsorbents
SEPABEADS SP70
SEPABEADS SP700
Cumulative pore volume
Logarithmic differential
Pore volume （mL/g）
SEPABEADS SP825
SEPABEADS SP850
DIAION HP21
DIAION HP20
Pore radius（Å）
β - Lactams
Peptides, Proteins
Macrolides
How to Use Synthetic Adsorbents
Column Adsorption
Method
Adsorption
l
Chromatographic
Operation
Time
l
Interaction between adsorbent
and molecules
Column
Washing
Adsorbent
Elution
Regeneration
Concentration
Method Development of Chromatographic
Separation
l
HPLC separation using analytical columns
n
n
l
Scale up separation using preparative columns
n
n
n
n
l
Selection of adsorbent type – polystyrenic or polymethacrylic
Selection of eluent system
Effect of particle sizes on separation
Effect of loading amount on separation
Adsorbent life, Regeneration method, Feasibility study
Equipment design
(Single or multi column system, SMB system)
Industrial Operation
n
Fine adjustment of conditions previously determined
Example of HPLC Analysis Equipment
Sample
Injector
n
n
l
CHP10M, CHP2MGM ( 4µm)
150 x 4.6mm I.D.
- Resin volume: 2.50ml
CHP5C, CHP2MG (10µm)
250 x 4.6mm I.D.
- Resin volume: 4.15ml
Column Oven
Column size
Column
l
Detector
Example of flow rate
n
n
150 x 4.6mm I.D.
- 0.5ml/min: LV=1.8m/h, SV=12
250 x 4.6mm I.D.
- 1.0ml/min: LV=3.6m/h, SV=14.4
Fraction
Collector
Example of Preparative Chromatography
Equipment
l
Example of preparative columns
n
Feed
Solution
Column
n
500 x 20mm I.D.
- Resin volume: 157ml
500 x 90mm I.D.
- Resin volume: 3,180ml
Water
l
Example of flow rate
n
n
500 x 20mm I.D.
- 6.28ml/min:
LV=1.2m/h, SV=2.4
500 x 90mm I.D.
- 63.6ml/min:
LV=0.60m/h, SV=1.2
Eluent
Regenerant
Pump
Detector
Fraction
Collector
Example of Packed Columns
Analytical column
CHP10M
150 x 4.6mm I.D.
Preparative column 1 Preparative column 2
CHP55A
500 x 32mm I.D.
(Max. Vol.: 400mL)
CHP55Y
500 x 90mm I.D.
(Max. Vol.: 3L)
Methods of Adsorption and Elution
l
Solvent Composition Change
n
Adsorption Process
Aqueous solution having the lowest solvent content
n
Elution Process
Pure solvent or Aqueous solution with high solvent composition
l
pH Change
n
Adsorption Process
Aqueous solution adjusted at pH region in which product ionic functionality
does not dissociate
n
Elution Process
Low medium buffer solution adjusted at pH region in which product ionic
functionality dissociates
Pressure Drop of Synthetic Adsorbents
Hydraulic Properties (Water, 25°C)
Head Loss (MPa/m-bed)
0.1
0.08
0.06
0.04
HP20
HP21
0.02
SP207
SP825
0
0
10
20
30
40
Linear Velocity (m/hr)
50
Swelling / Shrinkage of Synthetic Adsorbents
Swelling ratios of HP2MG for various
aqueous solvents (25°C)
1.4
1.4
1.3
1.3
Swelling Ratio
Swelling Ratio
Swelling ratios of HP20 for various
aqueous solvents (25°C)
1.2
MeOH
EtOH
IPA
Acetone
1.1
1.2
MeOH
1.1
AcCN
1
IPA
1
0
20
40
60
80
Solvent concentration (v/v %)
100
0
20
40
60
80
Solvent concentration (v/v %)
100
Advantages of Using Synthetic Adsorbents
l
From Analytical to Industrial Separations
n
n
l
Chemical Stability
n
l
With the use of same polymer structure performances
With same separation conditions
Stable in a wide pH range
Cost Effectiveness
n
n
Long operation life
Simple regeneration method with chemical at low ecological impact
Worldwide Network of DIAION ® / MCI ® GEL
http://www.diaion.com/
http://www.resindion.com/
Itochu Specialty
Chemicals
RESINDION
http://www.itochuhttp://www.itochu-sc.com/
MITSUBISHI
CHEMICAL
MCHK
TAI-YOUNG
CHEMICAL
RENSUI
ASIA
Manufacturing / Sales division
Sales division
Sales representative
http://www.diaion.com.tw/