Brief description of myiasis cases in three amphibian
Transcription
Brief description of myiasis cases in three amphibian
Herpetology Notes, volume 8: 287-290 (2015) (published online on 18 May 2015) Brief description of myiasis cases in three amphibian species from Atlantic Forest located in the central region of the State of Minas Gerais, Brazil Felipe Carvalho de Souza-Pinto1,*, Izabella Fernandes França2 and Cátia Antunes de Mello-Patiu2 Endoparatism in amphibians is widely observed and several cases have been described in literature (Bursey and DeWolf, 1998; Bolek and Coggins, 1998; Ghazi and Noorun-Nisa, 2005; Luque et al., 2005; Van Sluys et al., 2006; Santos et al., 2008; Nworah and Olorunfemi, 2011; Gonzalez et al., 2012; Santos et al., 2013; González & Hamann, 2014); however, there are just few reports of ectoparasites, wich are restricted to cases of parasitism by ticks (Sincok and Brum, 1997; Dantas-Torres et al., 2008), leeches (Loebmann et al., 2008) and some records of myiasis (Bolek and Coggins, 2002; Bolek and Javony, 2004; Hoskin and McCallum, 2007; Eaton et al., 2008; Medina et al., 2009; Travers and Townsend, 2010). In Brazil,only six cases of myiasis have been reported (Lopes, 1981; Souza et al., 1989; Eizemberg et al., 2008; Mello-Patiu and Luna-Dias, 2010; CarvalhoFilho et al,. 2010; Oliveira et al., 2012). Myiasis is defined as a condition caused by dipterous larvae that can invade the host’s tissue seeking to complete their development while they feed on its tissue (Pierce, 1981; Rey 1991, Linhares, 2000). In January/2011, during daytime transects in field work in Sabará, MG, Brazil, five specimens of the class Amphibia were found parasitized by larvae of Diptera. Three of them were individuals of Rhinella schneideri(Werner, 1894), one of Scinax fuscovarius(Lutz, 1925) and one of Scinax gr. ruber. Biocev Meio Ambiente, Rua Adolfo Radice 320, Mangabeiras CEP 30315-050, Belo Horizonte, MG, Brazil. 2 Universidade Federal do Rio de Janeiro, Museu Nacional, Departamento de Entomologia, Quinta da Boa Vista, 20940040, Rio de Janeiro, RJ, Brazil. * Corresponding author e-mail: [email protected] They were captured in border areas of seasonal semideciduous forest with a permanent stream, permanent pond and temporary puddles. Specimens were found resting and shortly after capture we perceived some round skin lesions (x¯ Diameter = 0.55 mm, SD = 0.1359, range = 0.42 to 0.74, N = 5) with infestation of larvae (Figure 1). The larvae were collected using a pair of iris tweezers and placed inethanol (Figure 2). After the larval removal and treatment with aqueous Polyvinyl Pyrrolidone Iodine to avoid infection, two individuals were reintroduced into the local habitat. The other three individuals captured did not survive this aforementioned 1 Figure 1. Specimen of Rhinella pombali with infestation of Diptera larvae. 288 Figure 2. Diptera larvae being removed from injury on Scinax gr. ruber. procedure and were fixed in a 10% formalin solution and subsequently kept in a 70% ethanol solution. The collected individuals were depositedin the scientific collection of the Herpetology Museum of Natural Sciences of PUC Minas in Belo Horizonte, MG, Brazil (MCNAM 17665, MCNAM 17666, MCNAM 17667). The larvae were identified as belonging to the family Sarcophagidae (Figure 3). However, due to the larval stage and the fixation process which interrupted their development, it was not possible to identify them to species level. This family has a worldwide distribution (Pape, 1996) occurring mainly in the tropics (Shewell, 1987; Pape, 1996). Figure 3. Sarcophagidae larvae removed from injury on Rhinella schneideri. Felipe Carvalho de Souza-Pinto et al. The decomposition process in vertebrates made by larvae from this specific group is also well known (Lopes, 1973; Monteiro-Filho and Penereiro, 1987; Salviano, 1996; Moura et al., 1997; Carvalho et al., 2000; Carvalho and Linhares, 2001); however, its parasitic action is small whencompared to other families of Diptera (Stevens et al., 2006) and yet poorly studied, especially for amphibians (Crump and Pounds, 1985; Hagman et al., 2005; Eizemberg et al., 2008; Medina et al,. 2009; Mello-Patiu and Luna-Dias, 2010). Interestingly, all individuals were captured during the daylight, even though they have nocturnal behavior, and all of them showed signs of activity. From this fact we can assume that the presence of the larvae may change their habits, probably causing irritation. As argued by Medina et al. (2009) it is presumable that larvae have parasitized specimens during daytime, when amphibian species are less active and the risk of flies being preyed is reduced. According to Stevens et al. (2006) Sarcophagidae larvae have lower host-specificity, hence showing a pattern of a short period of larval feeding generating superficial or cutaneous wounds, and relatively high levels of pathogenicity. Nonetheless, after analysis conducted in collected individuals of S. fuscovarius, internal larvae were found mostly in tissues and viscera reaching even muscles of the femoral region, though the abscesses were located near the axillary region which stands as a pattern usually found in larvae of Oestridae. However that may be related to the small size of the host. In individuals of R. schneideri, abscesses were located near the paratoid glands and in all individuals, larvae were collected below glands and within them, demonstrating the resistance of the larvae to toxins present in these areas. This is the first record of parasitism by larvae of Sarcophagidae documented for the genus Scinax and the first record of this kind of interaction between these genus and flies for the state of Minas Gerais. Myiasis cases are poorly documented due to the difficulty of recording the phenomenon due to the quick action of parasites (Bolek and Coggins, 2002; Bolek and Janovy, 2004; Mello-Patiu and Luna-Dias, 2010). For further works or even new records, we suggest to use the technique indicated by Mello-Patiu and LunaDias (2010), which consists to capture larvae after they leave hosts, and place them in a container with a soil sample to allowfurther development, metamorphosis and subsequently identification at species level of the imagos. Brief description of Myiasis cases in three amphibian species from Brazil Other authors also fix larvae when they are still immature.This procedure hinders an accurate species identification of larvae. Due to the large number of species in the Sarcophagidae family, difficulty of taxonomic identification and scarce knowledge, it is relevant to study the adult stages of these larvae to confirm the species. Acknowledgements. We thank Dr. Matthew Bolek, Dr. Brian Eaton and Dr. Fred Kraus for help and support, the Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA) for scientific collection permit, thescientific collection of the Herpetology Museum of Natural Sciences of PUC Minas for receiving the specimens, Msc. Cândida Radicchi de Oliveira Alméri and Rejane Protzner Sileiro for English review and editing and Dr. Conrado Aleksander Barbosa Galdino for the report confirming the species identification and the relevance of this paper. References Bolek, M.G., Coggins J.R. (1998): Endoparasites of Cope’s gray treefrog, Hylachrysoscelis, and western chorus frog, Pseudacrist. triseriata, from southeastern Wisconsin. Journal of the Helminthological Society of Washington65: 212–218. Bolek, M.G., Coggins, J.R. (2002): Observations on myiasis by the calliphorid, Bufolucilia silvarum, in the eastern American toad (Bufo americanus americanus) from southeastern Wisconsin. Journal of Wildlife Diseases 38: 598–603. Bolek, M.G., Janovy, J.J. (2004): Observations on myiasis by the Calliphorids, Bufolucilia silvarum and Bufolucilia elongata, in the wood frogs, Rana sylvatica from Southeastern Wisconsin. Journal of Parasitology 90: 1169–1171. Bursey, C.R., DeWolf, W.F. (1998): Helminths of the frogs, Rana catesbeiana, Rana clamitans, and Ranapalustris, from Coshocton County, Ohio. Ohio Journal of Science 98:28–29. Carvalho, L.M.L., Thyssen, P.J., Linhares, A.X., Palhares, F.A.B. (2000): A checklist of arthropods associated with pig carrion and human corpses in Southeastern Brazil. Memórias da Fundação Oswaldo Cruz 95: 135–138. Carvalho, L.M.L., Linhares, A.X. (2001): Seasonality of insect succession and pig carcass decomposition in a natural forest area in southeastern Brazil. Journal of Forensic Sciences 46: 604–608. Carvalho-Filho, F.S., Gomes, J.O., Maciel, A.O., Sturaro, M.J., Silva, K.R.A. (2010): Rhinella margaritifera (NCN). Parasites. Herpetological Review 41:479-480. Crump, M.L., Pounds, J.A. (1985): Lethal parasitism of an aposematic anuran (Atelopus varius) by Notochaeta bufonivora (Diptera: Sarcophagidae). Journal of Parasitology 71: 588-591. Dantas-Torres, F., Oliveira-Filho, E.F., Soares, A.M., Souza, B.O.F., Valença, R.B.P., Sá, F.B. (2008): Ticks infesting amphibians and reptiles in Pernambuco, Northeastern Brazil. Revista Brasileira de Parasitologia Veterinária17(4), 218-221. Eaton, B.R., Moenting, A.E., Paszkowski, C.A., Shpeley, D. (2008): Myiasis by Lucilia silvarum (Calliphoridae) in amphibian species in boreal Alberta, Canada. J. Parasitol. 94: 949-952. 289 Eizemberg, R., Sabagh, L.T., Mello, R.S. (2008): First record of myiasis in Aplastodiscus arildae (Anura: Hylidae) by Notochaeta bufonivora (Diptera: Sarcophagidae) in the Neotropical area. Parasitology Research 102: 329–331. Ghazi, R.R., Noorun-Nisa, K.A. (2005):Two new species of helminth parasites from frog (Rana tigrinaDaudin).Pakistan Journal of Zoology37(2): 107-111. Gonzalez, C.E., Hamann, M.I., Salgado, C. (2012) Study of Helminth Parasites of Amphibians by Scanning Electron Microscopy. In: Scanning Electron Microscopy, p. 267-294. Kazmiruk, V., Ed, Manhattan, InTech. González C.E., M.I. Hamann. 2014. Schrankiana chacoensis sp. nov. (Nematoda: Atractidae) from Leptodactylus bufonius Boulenger, 1894 (Anura: Leptodactylidae) from Argentina. Journal of Natural History 48 (1-2): 35-49. DOI: 10.1080/002 22933.2013.800608. Hagman, M., Pape, T., Schulte, R. (2005): Flesh fly myiasis (Diptera, Sarcophagidae) in Peruvian poison frogs genus Epipedobates (Anura, Dendrobatidae). Phyllomedusa 4: 69–73. Hoskin C.J., McCallum, H. (2007): Phylogeography of the parasitic fly Batrachomyia in the Wet Tropics of north-east Australia, and susceptibility of host frog lineages in a mosaic contact zone. Biological Journal of the Linnean Society 92: 593–603. Linhares, A.X. (2000): Miíases. In: Parasitologia humana, p. 350357. Neves, D.P.; Mello, A.L; Genaro, O.; Linardi, P.M., Eds. São Paulo, Athneu. Loebmann, D., Solé, M., Kwet, A. (2008): Predation on spawn and adults of Chaunus dorbignyi (Duméril & Bibron, 1841) (Amphibia, Anura) by leeches (Hirudinea) in southern Brazil. Amphibia 7(1): 31-34. Lopes, H.S. (1973): Collecting and rearing sarcophagid flies (Diptera) in Brazil during forty years. Anais da Academia Brasileira de Ciências 45: 279–299. Lopes, H. S. (1981): Notes on American Sarcophagidae (Diptera). Revista Brasileira de Biologia 41: 149–152. Luque, J.L., Martins, A.N., Tavares, L.E.R. (2005): Community structure of metazoan parasites of the yellow Cururu toad,Bufo ictericus (Anura, Bufonidae) from Rio de Janeiro, Brazil.Acta Parasitologica50(3): 215-220. Medina, D., Rivera, M., Cossio, R., Medina, E., Bermudez, S. (2009): Primer registro de miiasis por Sarcophagidae (Diptera: Oestroidea) em Hyalinobatrachium fleischmanni (Anura: Centrolenidae) de Panamá . Revista Mexicana de Biodiversidad 80: 263–264. Mello-Patiu, C.A., Luna-Dias, C. (2010): Myiasis in the Neotropical Amphibian Hypsiboas beckeri (Anura: Hylidae) by a new species of Lepidodexia (Diptera: Sarcophagidae). Journal of Parasitology 96: 685-688. Monteiro-Filho, E.K.L.A., Penereiro, J.L. (1987): Estudo da decomposição e sucessão sobre uma carcaça animal numa área do estado de São Paulo, Brasil. Revista Brasileira de Biologia 47: 289–285. Moura, M.O., Carvalho, C.J.B., Monteiro-Filho, L.A. (1997): A preliminary analysis of insects of medico-legal importance in Curitiba, State of Paraná. Memórias do Instituto Oswaldo Cruz 92: 269–274. Nworah, D.C., Olorunfemi, O.J. (2011): The helminth parasitofauna of Bufo regularis (Reuss) in Awka, Anambra State, Nigeria. 290 International Journal for Parasitology - Elsevier 3(2): 26-30. Oliveira, R.M., Mendes, C.V.M., Ruas, D.S., Solé, M., Pinho, L.C., Rebouças, R. (2012): Myiasis on Hypsiboas atlanticus (Caramaschi and Velosa, 1996) (Anura: Hylidae) from southern Bahia, Brazil. Herpetology Notes 5: 493-494 Pierce, A.W (1981): Myiasis. In: Medical microbiology and infectious diseases, p. 1704-1710. Braud, A.I., Ed., Philadelphia, W.B. Saunders Company. Pape, T. (1996). Catalogue of the Sarcophagidae of the world (Insecta: Diptera).Memoirs of Entomology, International 8: 558 p. Rey, L. (1991): Parasitologia, 2nd Edition. Rio de Janeiro, Editora Guanabara Koogan. Salviano, R.J.B. (1996): Sucessão de Diptera Caliptrata em carcaça de Sus scrofaL. Unpublished Masters thesis. Universidade Federal Rural do Rio de Janeiro, Rio de Janeiro, 124 pp. Santos VGT, Amato SB, Borges-Martins M (2013) Community structure of helminth parasites of the “Cururu” toad, Rhinella icterica (Anura: Bufonidae) from southern Brazil. Parasitol Res 112:1097–1103. Santos, J. N., Giese, E. G., Maldonado Jr, A., Lanfredi, R. M. (2008): A new species of Oswaldocruzia (Molineidae: Nematoda) in Chaunusmarinus (Amphibian: Bufonidae) (Linneaus, 1758) from Brazil. Journal of Parasitology 94: 264–268. Shewell, G.E. (1987): Sarcophagidae. In Manual of Nearctic Diptera, p. 1159-1186. McAlpine, J.F., Peterson, B.V., Shewell, G.E., Teskey, H.J., Vockeroth, J.R., Wood, D.M., Eds. Ottawa, Agriculture Canada Research Branch Monograph. Sinkoc, A.L., Brum, J.G.W. (1997): Ocorrência de Amblyomma fuscum Neumann, 1899 e Amblyomma humerale Koch, 1844 (Acari: Ixodidae) em Bufo arenalis no estado de São Paulo, Brasil. Ciência Rural 27(2): 339-340. Felipe Carvalho de Souza-Pinto et al. Souza, JR., F.L.S., Souza, C.W.O., Hipolito, M., Baldassi, L., Martins, M.L. (1989): Cases of buccal myiasis in the bullfrog (RanacatesbeianaShaw, 1802), with larvae of Notochaetasp. Aldrich, 1916 (Diptera: Sarcophagidae) in São Paulo, Brazil. Memórias do Instituto Oswaldo Cruz 84: 517–518. Stevens, J.R., Wallman, J.F., Otranto, D., Wall, R., Pape, T. (2006). The evolution of myiasis in humans and other animals in the Old and New Worlds (part II): biological and life-history studies. Trends in Parasitology, 22: 181-188. Travers, S.L., Townsend, J.H. (2010): Myiasis on a Neotropical leaf frog Agalychnis saltator Taylor, 1955. Herpetology Notes 3: 355-357. Van Sluys, M., Schittini, G. M., Marra, R. V., Azevedo, A. R. M.,Vicente, J. J., Vrcibradic, D. (2006): Body size, diet and endoparasites of the microhylid frog Chiasmocleis capixaba in an Atlantic Forest area of southern Bahia state, Brazil. Brazilian Journal of Biology 66(1A): 167–173. Accepted by Mirco Solé