Nov-Dec 2015 - MicrobeHunter Microscopy Magazine

Microbe
Hunter
Microscopy Magazine
Insects
Crystals
ISSN 2220-4962 (Print)
ISSN 2220-4970 (Online)
Volume 4, Number 11
November-December 2014
The Magazine for the
Enthusiast Microscopist
http://www.microbehunter.com
Lichens
November-December 2014 - MicrobeHunter Microscopy Magazine - 1
CONTENTS
The magazine for the enthusiast microscopist
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Water Life
Luca Monzo
The Magazine is a non-commercial project.
Volume 4, Number 1, Nov-Dec 2014 (rev 1)
ISSN 2220-4962 (Print)
ISSN 2220-4970 (Online)
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Download: Microbehunter Microscopy Magazine can be downloaded from:
http://www.microbehunter.com
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Print version: The printed version can be ordered at: http://microbehunter.magcloud.com
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Publisher and editor: Oliver Kim, Ziegeleistr.
10-3, A-4490 St.Florian, Austria
Email: [email protected]
Web: http://www.microbehunter.com
Tel.: +43 680 2115051
Copyright: By submitting articles and pictures,
the authors have confirmed that they are the full
copyright owners of the material, unless specified otherwise. Authors are responsible for obtaining permission for copyrighted work that they
do not own. Creative commons and public domain images are indicated with a small text next
to the image or in the caption. The copyright of
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(unless specified). If you feel that your copyright
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Editorial: Article and image submissions are
welcome and should be sent to:
[email protected].
For submission guidelines, consult the website
at: http://www.microbehunter.com/submission
Disclaimer: Articles that are published in Microbehunter Microscopy Magazine and the blog
do not necessarily reflect the position or opinion
of the publisher. The publication of these articles
does not constitute an endorsement of views
they may express. The authors themselves are
responsible for the contents. Advice provided in
Microbehunter Microscopy Magazine is provided
as a service and as a recreational resource and
neither the authors nor the publisher can be held
liable and responsible for any errors, omissions
or inaccuracies, or for any consequences
(health, hardware, etc.) arising from the use of
information of this magazine and the blog (or
anything else). Conduct all lab work and (microscopy) hardware modifications at your own
risk and always follow the instructions of the
manufacturers.
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Plant cross sections
Oliver Kim, Rodney Brightwell
Oxalic acid
Anthony Thomas
Drosophila and Diatom
Anthony Thomas
Plant impressions
Mandfred Rath
Crystals in polarized Light
Jeff Clinedinst
Lichens and Moss
James D. Crosby
Insects
Anthony Thomas
Volvox
Anthony Thomas
Water Life from an Aquarium
Mandfred Rath
Pond Life
R. Nassar
Water Life
Luca Monzo
Maple Leaf Veins
Oliver Kim
Wasp and Hornet
Manfred Rath
Vitamin C crystals
Front Cover: Vitamin C (Oliver Kim)
Left image: Anthony Thomas
Middle image: Matej Pašák
Right image: James D. Crosby
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Back cover: Leaf veins
Microbehunter Microscopy Magazine
Water Life
GALLERY
The images on this double page show water fleas of my little
water tank. The original source is a fountain located in the
public garden of Udine, Italy (see photo on the left). I used a
piece of black paper to obtain oblique illumination on some
images. I processed them with Helicon Focus or ACDSee
software.
By Luca Monzo
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GALLERY
Plant cross sections
Cross section through the root of a Lupinus plant. Oliver Kim
Wood slice section.
This old wood was
dug up out of our pond
and was aprox. 10-12
feet below ground level. It
apears to have charcoal
traces in it which are visible
lower right. The microscope
used was a Bausch and Lomb
Dynazoom at 120x.
Rodney Brightwell
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Oxalic acid
GALLERY
Oxalic Acid under polarized
light. Olympus BH2 10x S Plan
objective + 1.25x intermediate
tube + 2.5x relay tube; Nikon
D90, flash
Anthony Thomas
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GALLERY
Drosophila and Diatom
Anterior end of a young pupa of a Fruit
Fly (Drosophila). You can still see the
larval mouthparts. Specimen in glycerine on a slide. Nikon 20x ELWD objective on a bellows.
Close-up of the Drosophila breathing
tubes. Nikon 40x LWD objective on a
bellows.
These are photosynthetic diatoms that
are common in a local drainage ditch.
Olympus BH2, 20x SPlan Apochromatic
objective, 1.25x intermediate lens, 2.5x
relay lens, DIC. Nikon D90 camera.
By Anthony Thomas
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Plant impressions
GALLERY
Anchoring roots of ivy,
stack of 25 images
made with Combine ZP.
A leaf cast of horseradish done by using clear
nail varnish. It is a mix
of Rheinberg- and
oblique illumination.
By Manfred Rath
Send images to [email protected] - November-December
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GALLERY
Crystals in Polarized Light
Salicylic acid, polarized.
Amscope T490, 10x, Canon 7D,
stack of 3 images in CombineZP,
cropped.
Hydroquionone melt, recrystalized
under the cover slip. Polarized with
added polypropylene film as
waveplate.
Amscope T490, 10x, Canon 7D,
stack of 3 images in CombineZP.
Sulfur melt, recrystalized under the
cover slip, polarized. Amscope T490,
10x, Canon 7D.
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Crystals in Polarized Light
GALLERY
Top: Sulfur melt,
recrystalized under cover
slip, polarized. Amscope
T490, 10x, Canon
7D, stitched together from
several images.
Bottom: Sulfur melt,
recrystalized under cover
slip, polarized. Amscope
T490, 10x, Canon
7D, stitched together from
several images.
All images on this double
page by Jeff Clinedinst
http://www.jclinedinst.c
om
Send images to [email protected] - November-December
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GALLERY
Lichens
Top and right: Lichen from King’s
Canyon, CA
Page 23, top: Easter moss
Page 24, bottom: “The lonely
sporophyte” (moss).
All images on page 10 and 11 by
James D. Crosby
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Moss
GALLERY
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GALLERY
Insects
Left: "Spiky-hair fly" mounted in Canada
Balsam, taken with a Nikon 63mm El
Nikkor enlarging lens (reversed) on
bellows.
Bottom: Close-up of head, taken with a
Nikon 10x CF N Plan Achromat objective
on bellows.
By Anthony Thomas
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Volvox
GALLERY
A plate of 9 images of a Volvox. Each is a single image taken using a 10x Olympus S
Plan objective + 2.5x NFK relay lens. Volvox is a round ball, impossible to get it all in
focus with one image.
By Anthony Thomas
Send images to [email protected] - November-December
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GALLERY
Water Life from an Aquarium
Impressions from an aquarium.
By Manfred Rath
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Pond Life
GALLERY
Top: an ostracod (seed shrimp)
Center left: Pseudodifflugia sp. and diatom, phase contrast, 40x
objective.
Lower left: Pseudodifflugia sp., 20x objective, phase contrast:
Center right: Cyanobacteria (the best I could determine was that
this is of Genus Microcistis, but not sure). It was from pond water
that I collected from a very small pond in the neighborhood many
months ago and kept in a glass jar). Objective 20x, bright field
illumination.
By R. Nassar
Send images to [email protected] - November-December
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GALLERY
Water Life
Paramecium bursaria taken with a Bresser trinocular microscope
(model Trino Researcher II) and a Canon EOS 350 at 1600 ISO.
Objective 40x with WF 10x eyepiece
By Luca Monzo
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Water Life
GALLERY
Top: Pedastrum sp., a green alga
Top right and center right: Ostracod
(seed shrimp)
Bottom: the Pinnularia sp. Diatom
All images taken with a Bresser
trinocular microscope (model Trino
Researcher II) and a Canon EOS
350D.
By Luca Monzo
Send images to [email protected] - November-December
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GALLERY
Leaf Veins
Veins of a maple leaf. A maple
leaf was boiled for several
hours to soften the tissue.
The leaf was placed on a flat
surface and the soft tissue
was carefully removed with a
soft brush to expose the leafveins. This procedure does
not work with all types of
leaves and experimenting is
necessary. Remaining
chlorophyll was removed
from the veins with alcohol
and the leaf was re-hydrated
with water to compensate the
shrinking. The veins were
then scanned with a flatbed
scanner at high resolution.
By Oliver Kim
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Wasp and Hornet
GALLERY
Top: image of a wasp.
Bottom: Portrait of a
hornet. Stack of 20
images made with
Combine ZP ("all
methods"). Equipment:
Stereo microscope
(Lomo MBS10).
By Manfred Rath
Send images to [email protected] - November-December
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What’s this? Answer on page 2.
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