YMC - Hichrom

HICHROM
Chromatography Columns and Supplies
LC COLUMNS
YMC
Catalogue 9
Hichrom Limited
1 The Markham Centre, Station Road
Theale, Reading, Berks, RG7 4PE, UK
Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484
Email: [email protected] www.hichrom.co.uk
YMC
LC Columns – YMC
YMC
YMC-Triart
inorganic
siloxane layer
• Multi-layer hybrid material
• Innovative micro-reactor technology
• Multi-stage endcapping
• High pH and temperature stability
• 1.9, 3 and 5μm particle sizes
YMC-Triart phases are based on a multi-layered hybrid material, manufactured using innovative micro-reactor
technology. The combination of this layered hybrid particle with the precise microreactor granulation and the
multi-stage endcapping procedures, produces a versatile material with outstanding chemical and physical durability,
plus excellent chromatographic performance with acidic, basic and neutral compounds.
organic
siloxane layer
Phase Specifications1
YMC-Triart C18
YMC-Triart C8
YMC-Triart Diol-HILIC
Organic/inorganic silica
Organic/inorganic silica
Organic/inorganic silica
C18
C8
Diol
1.9, 3, 5
1.9, 3, 5
1.9, 3, 5
120
120
120
Polymeric type
Polymeric type
-
Multi-stage hybrid groups
Multi-stage hybrid groups
-
C18: 16 (20% in total)
C8: 7 (11% in total)
-
1-12
1-12
2-10
pH 1-7:70°C, pH 7-12: 50°C
pH 1-7:70°C, pH 7-12: 50°C
50°C
Base Material
Ligand
Particle Size (μm)
Pore Size (Å)
Bonding
Endcapping
Carbon Load (%)
pH Range
Temperature Range
1
Other Triart phases are available. See page 6
Features of YMC-Triart
• Homogeneous and uniform particles. Use of microreactor technology produces homogeneous and uniform particles that result in low operating
pressure and reproducible surface modification, leading to excellent column-to-column and lot-to-lot reproducibility.
• Multi-stage endcapping. YMC-Triart uses an innovative endcapping procedure for surface modification. Using a stepwise, multi-stage process with
a number of compounds with different reactivities, the maximum number of silanols can be capped.
• Balanced hydrophobicity. YMC-Triart exhibits a balanced hydrophobicity, making it more retentive than other hybrids.
• Excellent durability. YMC-Triart C18 and C8 show excellent chemical stability. Even at high pH (up to pH 12) or high temperature conditions
(up to 70°C), the column lifetime is greater than for conventional C18 and C8 columns.
For free technical advice and support email [email protected]
• Method transfer between HPLC ↔ UHPLC. YMC-Triart phases provide matching chromatographic behaviour for all particle sizes. This ensures
identical selectivity and excellent peak shape for basic compounds for all particle sizes. YMC-Triart allows predictable scale-up from UHPLC to
conventional HPLC and even to semi-preparative LC and vice versa. Figure 1 illustrates method transfer from a 5μm, 150 x 2.0mm YMC-Triart C18
to a 1.9μm, 50 x 2.0mm column. Resolution is maintained but run time has been decreased from 55mins to 6mins.
266
A
1
4.5 MPa (650 psi)
5% B (0-3 min)
5-30% B (3-10 min)
30-45% B (10-55 min)
0.2 ml/min
2
0
3
10
7
4
20
55 min
8 9
5 6
30
40
Mins
55
50
1
B
2
37 MPa (5400 psi)
5% B (0-0.3 min)
5-30% B (0.3-1.0 min)
30-45% B (1.0-6.0 min)
0.6 ml/min
7
3
8 9
4
6 min
6
5
0
1
2
3
Figure 1. Method transfer between HPLC and UHPLC
4
5
6
Mins
1. Oxine copper
2. Asulam
3. Thiram
4. Triclopyr
5. Mecoprop
6. Flazasulfuron
7. Siduron
8. Halosulfuron-methyl
9. Azoxystrobin
Columns:A: YMC-Triart C18 (5μm, 150 x 2.0mm)
B: YMC-Triart C18 (1.9μm, 50 x 2.0mm)
Eluent: A: 0.1% formic acid in H2O
B: 0.1% formic acid in CH3CN
Temperature: 40°C
Detection: UV, 240nm
YMC
Features of YMC-Triart (continued)
• Improvement of loadability. YMC-Triart C18 can tolerate larger injection volumes containing strong solvents (eg. acetonitrile), whilst maintaining
better peak shape than traditional silica-based columns. This can be important for difficult to solubilise samples that need higher concentrations of
organic solvent for solubilisation prior to analysis. Figure 2 illustrates the influence of injection volume on peak shapes.
• LC-MS compatibility. Extremely low levels of column bleed lead to reduced background noise and hence lower levels of detection. In Figure 3, no
bleed is observed in the test of total ion current measured by LC-MS with blank (no column) or with YMC-Triart C18.
LC Columns – YMC
YMC-Triart (continued)
Solvent for sample: acetonitrile
Injection volume: 1.0µl, 1.5µl, 2.0µl, 3.0µl
Column: 5μm, 50 x 2.0mm
Eluent: A: Water – formic acid (100:0.1)
B: CH3CN – formic acid (100:0.1)
5 to 100% B from 0.5 to 2.5min
Flow rate: 0.4ml/min
Temperature: 40°C
Detection: UV, 275nm
Column: YMC-Triart C18 (5μm, 50 x 2.0mm)
Eluent: A: 0.1% formic acid in H2O
B: 0.1% formic acid in CH3CN
Gradient: 5% B (0-1min), 5-100% B (1-5min),
100-5% B (10-10.1min), 5% B (10.1- 12.5min)
Flow rate: 0.4ml/min
Temperature: 40°C
Detection: ESI +ve, TIC (mass range 50-1000)
1. Propranolol
2. Verapamil
YMC-Triart C18
YMC-Triart C18
No column
Silica-based C18
Figure 2. Influence of injection volume on peak shapes*
Figure 3. LC-MS compatibility*
*The comparative separations and data presented here may not be representative for all applications.
Hardware for YMC-Triart 1.9μm columns
YMC-Triart columns packed with 1.9μm particle size phases are provided in PT-hardware, which is compatible with Waters UPLC® systems and Agilent
equipment. This enables optimised chromatographic separations to be achieved when using these and other instruments.
Ordering Information
When ordering please replace ‘XX’ with TA for Triart C18, TO for Triart C8 and TDH for Triart Diol-HILIC.
1.9μm YMC-Triart C18, C8 and Diol-HILIC Columns4
Column Length1 (mm)
Column i.d. (mm)
Guard Cartridges2
20
30
50
100
150
(5mm length)
2.0
XX12SP9-0202PT
£469
XX12SP9-0302PT
£454
XX12SP9-0502PT
£469
XX12SP9-1002PT
£483
XX12SP9-1502PT
£528
XX12SP9-E5Q1CC
£409
3.0
-
XX12SP9-0303PT
£469
XX12SP9-0503PT
£469
XX12SP9-1003PT
£483
-
XX12SP9-E503CC
£409
3µm and 5μm YMC-Triart C18, C8 and Diol-HILIC Columns4
Column i.d. (mm)
Column Length1 (mm)
3μm Particle Size
50
150
250
(10mm length)
2.0
XX12S03-0502WT £320 XX12S03-1002WT £320 XX12S03-1502WT £379 XX12S03-2502WT £394 XX12S03-01Q1GC £231
3.0
XX12S03-0503WT £298 XX12S03-1003WT £342 XX12S03-1503WT £387
4.6
XX12S03-0546WT £305 XX12S03-1046WT £357 XX12S03-1546WT £417 XX12S03-2546WT £454 XX12S03-0104GC £231
5μm Particle Size
50
100
150
-
XX12S03-0103GC £231
250
2.0
XX12S05-0502WT £312 XX12S05-1002WT £357 XX12S05-1502WT £372 XX12S05-2502WT £394 XX12S05-01Q1GC £231
3.0
XX12S05-0503WT £298 XX12S05-1003WT £335 XX12S05-1503WT £357 XX12S05-2503WT £402 XX12S05-0103GC £231
4.6
XX12S05-0546WT £305 XX12S05-1046WT £350 XX12S05-1546WT £379 XX12S05-2546WT £431 XX12S05-0104GC £231
3
4
Hichrom Limited
Other dimensions available 2
3/pk. Requires holder XPCHUHP (£364) 1
100
Guard Cartridges3
5/pk. Requires holder XPGCH-Q1 (£132) - see p. 272 for further information
YMC-Triart Phenyl and PFP also available - please enquire
YMC-Triart preparative 10 and 15μm bulk material also available – please enquire.
www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660
267
LC Columns – YMC
YMC
YMC ProFamily
• Ultra high purity silica
• C18, C8 and C4 bonded phases
• Hydrosphere for aqueous stability
• ProC18 and Hydrosphere C18 in 2, 3 and 5μm phases
The ProFamily is YMC’s range of state-of-the-art ultra high purity silica phases (99.999%). They are manufactured using high specification silica with
extremely low individual metal ion contents (typically <10ppm). Test certificates supplied for both the bulk material and the column characterise the
quality and reproducibility of these products.
YMC ProFamily Phases
YMC Phase
1
Code
Description
Particle Size
(μm)
Pore Size
(Å)
Carbon Load
(%)
pH Range
21, 3, 5
120
16
2-8
Applications
Fat soluble vitamins, antioxidants,
acidic, neutral, basic and chelating
compounds
ProC18
AS
Ultrapure silica base with
low residual non-specific
interactions
ProC18RS
RS
High carbon load with
polymeric bonding C18
3, 5
80
22
1-102
Hydrosphere C18
HS
Can be used in 100%
aqueous eluent
21, 3, 5
120
12
2-8
ProC8
OS
Latest generation C8
with very low residual
non-specific interactions
3, 5
120
10
2-7.5
Acidic, neutral, basic and chelating
compounds, drugs and metabolites
ProC4
BS
Ultrapure silica base,
endcapped
3, 5
120
7
2-7.5
Polar acidic, basic and chelating
compounds, polar peptides
YMC-UltraHT phases
2
Wide range of samples including
acidic and basic compounds
Strongly polar compounds, antibiotics,
catecholamines, nucleic acids, water
soluble vitamins
It is recommended to use at least 10% organic solvent composition near the pH limits and over 50% at pH levels above pH 9.0 to preserve column lifetimes
Phases in the YMC ProFamily are ideal for applications
in both the pharmaceutical and biotechnology industries.
ProC18RS is specifically designed for improved retention
of non-polar, hydrophobic compounds. The high carbon
load and surface area (510m2/g) and extended pH
stability provide further selectivity options for demanding
separations, including LC-MS and method development.
The enhanced steric selectivity of this phase enables
positional isomers to be separated. Hydrosphere C18
contains a hydrophilic C18 surface for enhanced polar
selectivity and operation under 100% aqueous eluent
conditions. Typical applications include nucleic acid bases,
nucleotides, organic acids, catecholamines, vitamins etc.
Figure 4. Comparison of YMC ProFamily separation selectivity
For free technical advice and support email [email protected]
Figure 4 compares the selectivity characteristics of the three C18 phases using hydrophobicity and hydrogen-bonding ability as indicators. This plot
highlights the contrasting separation features of these phases. Figure 5 shows a comparison of the differing hydrophobicities and hydrogen bonding
capacities of each phase in combination with basic substances.
268
1
Column: 50 x 2.0mm
Eluent: CH3OH - 10mM CH3COONH4 (55:45)
Flow rate: 0.2ml/min
Temperature: 37˚C
Detection: UV at 254nm
ProC4
3
2
1
ProC8
4
1
2
5,4
Hydrosphere C18
1
ProC18
2
ProC18RS
2
3
5
1. Toluene
2. Amitriptyline HCl
3. Progesterone
4. Propylbenzene
5. Acenaphthene
1
3
3
2
3
4
4
5
4
5
5
0
10
Mins
lowest hydrophobicity
0
10
20
lower hydrophobicity
Mins
0
10
20
balanced hydrogen bonding,
capacity and hydrophobicity
30 Mins
0
10
20
30
highest carbon load
Figure 5. Comparison of characteristics of ProFamily phases
40 Mins
0
10
20 Mins
high polar recognition
YMC
• ProC18 and Hydrosphere C18 phases
• Same selectivity as the corresponding 3 and 5μm phases
• Use with UHPLC or conventional HPLC systems
• Fully downscalable from 3 and 5μm to 2μm
The YMC-UltraHT ProC18 and Hydrosphere C18 bonded phases are based on high efficiency 2μm spherical silica particles, of the same pore size, surface
area and carbon content as the equivalent 3 and 5μm products (see page 268). They are specifically designed for high speed and high throughput analyses
in various fields including pharmaceuticals, foods and environmentals. YMC-UltraHT columns exhibit low pressure drop and high flow rates can be
achieved without generating excessive back pressure and without the need for specialised equipment.
LC Columns – YMC
YMC-UltraHT (2μm)
Due to the production process used to manufacture the YMC ProFamily, methods can easily be downscaled without changes to selectivity. Figure 6 shows
the transfer of a separation of a polar isoflavone mixture from a 5μm, 150 x 4.6mm i.d. YMC Hydrosphere C18 column to a 2μm, 50 x 2.0mm i.d.
YMC-UltraHT Hydrosphere C18 column.
A. Conventional LC method YMC Hydrosphere C18 5µm
B. Ultra fast LC method YMC-UltraHT Hydrosphere C18 2µm
Column: YMC Hydrosphere C18 (5µm, 150 x 4.6mm)
Eluent: A: H2O - CH3CO2H (100:3)
B: CH3CN - CH3CO2H (100:3)
Gradient: 15 - 35% B in 30 mins
Flow rate: 1.0ml/min
Temperature: 35°C
3
9
1
2
0
6
4 5
6
1. Daidzin
2. Glycitin
3. Genistin
4. 6’-O-Malonyldaidzin
5. 6’-O-Malonylglycitin
6. 6’-O-Acetyldaidzin
7. 6’-O-Acetylglycitin
8. 6’-O-Malonylgenistin
9. Daidzein
10. Glycitein
11. 6’-O-Acetylgenistin
12. Genistein
12
10
11
78
12
18
24
Mins
Column: YMC-UltraHT Hydrosphere C18 (2µm, 50 x 2.0mm)
Eluent: A: H2O - CH3CO2H (100:3)
B: CH3CN - CH3CO2H (100:3)
Gradient: 12.5 - 30% B in 3.3 mins.
Flow rate: 0.9ml/min
Temperature: 35°C
1
3
9
2
4
0
5
1
12
10
11
6 7
8
2
3
Mins
Figure 6. Downscaling of isoflavone separation
Ordering Information – YMC ProFamily
YMC Phase
3μm
50 x 2.1
AS12S03-05Q1QT
ProC18
£335
RS08S03-05Q1QT
ProC18RS
£312
HS12S03-05Q1QT
Hydrosphere C18
£312
OS12S03-05Q1QT
ProC8
£312
BS12S03-05Q1QT
ProC4
£312
5μm
50 x 2.1
AS12S05-05Q1QT
ProC18
£327
RS08S05-05Q1QT
ProC18RS
£312
HS12S05-05Q1QT
Hydrosphere C18
£312
OS12S05-05Q1QT
ProC8
£312
BS12S05-05Q1QT
ProC4
£312
YMC-UltraHT Phase
2μm
ProC18
Hydrosphere C18
1
100 x 2.1
AS12S03-10Q1QT
£335
RS08S03-10Q1QT
£335
HS12S03-10Q1QT
£335
OS12S03-10Q1QT
£335
BS12S03-10Q1QT
£335
150 x 2.1
AS12S05-15Q1QT
£387
RS08S05-15Q1QT
£387
HS12S05-15Q1QT
£387
OS12S05-15Q1QT
£387
BS12S05-15Q1QT
£387
30 x 2.0
£537
AS12S02-0302WT
HS12S02-0302WT
30 x 3.0
-
150 x 2.1
AS12S03-15Q1QT
£402
RS08S03-15Q1QT
£402
HS12S03-15Q1QT
£402
OS12S03-15Q1QT
£402
BS12S03-15Q1QT
£402
250 x 2.1
AS12S05-25Q1QT
£431
RS08S05-25Q1QT
£409
HS12S05-25Q1QT
£409
OS12S05-25Q1QT
£409
BS12S05-25Q1QT
£409
50 x 2.0
£512
AS12S02-0502WT
HS12S02-0502WT (£521)
50 x 3.0
£521
AS12S02-0503WT
HS12S02-0503WT
(5/pk, 10 x 4mm)
50 x 4.6
AS12S03-0546WT
£320
RS08S03-0546WT
£312
HS12S03-0546WT
£320
OS12S03-0546WT
£312
BS12S03-0546WT
£312
50 x 4.6
AS12S05-0546WT
£320
RS08S05-0546WT
£312
HS12S05-0546WT
£320
OS12S05-0546WT
£312
BS12S05-0546WT
£312
100 x 4.6
AS12S03-1046WT
£305
RS08S03-1046WT
£357
HS12S03-1046WT
£372
OS12S03-1046WT
£357
BS12S03-1046WT
£327
150 x 4.6
AS12S05-1546WT
£387
RS08S05-1546WT
£379
HS12S05-1546WT
£402
OS12S05-1546WT
£379
BS12S05-1546WT
£379
Column Dimensions (mm)
75 x 2.0
£529
AS12S02-L502WT
HS12S02-L502WT
75 x 3.0
£529
AS12S02-L503WT
HS12S02-L503WT
Use with universal holder XPGCH-Q1 (£132) or direct connect holder XPGCS-Q1 (£132) – see p. 272. 2.1mm i.d. guard cartridges also available
150 x 4.6
AS12S03-1546WT
£446
RS08S03-1546WT
£431
HS12S03-1546WT
£446
OS12S03-1546WT
£431
BS12S03-1546WT
£431
250 x 4.6
AS12S05-2546WT
£454
RS08S05-2546WT
£454
HS12S05-2546WT
£454
OS12S05-2546WT
£454
BS12S05-2546WT
£454
100 x 2.0
£537
AS12S02-1002WT
HS12S02-1002WT
100 x 3.0
£537
AS12S02-1003WT
HS12S02-1003WT
2
AS12S03-0104GC
£245
RS08S03-0104GC
£245
HS12S03-0104GC
£245
OS12S03-0104GC
£245
BS12S03-0104GC
£245
AS12S05-0104GC
£245
RS08S05-0104GC
£245
HS12S05-0104GC
£245
OS12S05-0104GC
£245
BS12S05-0104GC
£245
150 x 2.0
£678
AS12S02-1502WT
HS12S02-1502WT
150 x 3.0
£711
AS12S02-1503WT
HS12S02-1503WT
Other dimensions (eg. 1.0mm i.d.) also available
www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660
Hichrom Limited
ProC18
Hydrosphere C18
Guard Cartridges1
Column Dimensions2 (mm)
269
For free technical advice and support email [email protected]
LC Columns – YMC
YMC
270
YMC ‘Classical’ Phases
YMC manufacture a wide range of ‘classical’ and speciality bonded phases for reversed-phase, normal-phase and HILIC methods. The traditional
ODS-A, ODS-AL, ODS-AM and ODS-AQ phases are well documented in the scientific literature. The series of three J’sphere phases offers alternative
hydrophobicities due to variation in degree of ODS substitution. The Polyamine ll material is a mixed secondary and tertiary amino derivative, covalently
bonded onto 5μm particle size silica. This phase offers increased lifetime and alternative selectivity for carbohydrate applications compared with
conventional primary amino materials. The PVA-SIL material is prepared by bonding the silica support with a monomolecular polymer coating of vinyl
alcohol. It offers an excellent alternative selectivity to silica and can be used over a wider pH range.
1
Particle1
Size (μm)
Pore Size
(Å)
Carbon
Load (%)
pH Range
C30 ligands. Proprietary
polymeric bonding chemistry
3, 5
proprietary
-
2-6
AA
Traditional high performance C18
3, 5
120, 200,
300
17, 12,
7
2-7.5
Pharmaceuticals, vitamins, amino
acids, peptides, general purpose
phase
AM
Strict QC controlled production
provides high performance C18
column for validated methods
3, 5
120
17
2-7.5
Purines, phenols, PTC-amino acids,
angiotensins, alkaloids
ODS-AQ
AQ
‘Hydrophilic’ endcapping
for 100% aqueous eluents,
increased retention of polar
compounds
3, 5
120, 200
14, 11
2-7.5
Strong polar compounds,
pharmaceuticals, antibiotics,
peptides and proteins, nucleic acids
ODS-AL
AL
Traditional C18 for ‘mixed-mode’
separations
3, 5
120
17
2-7.5
Tocopherols, fat soluble vitamins,
disinfectants
J’sphere
JH, JM, JL
C18 family with different
hydrophobicity
4
80
22, 14, 9
(JH, JM, JL)
6
proprietary
C18=10%
2-13
Phenols, anilines, peptides in
high pH, pharmaceuticals,
quaternary amines
YMC Phase
Code
Carotenoid
CT
ODS-A
ODS-AM
Description
Applications
Isomeric carotenes, retinols,
steroids, fat-soluble vitamins
1-9 (JH),
Positional isomers, complexing
2-7.5 (JM, JL) agents, pharmaceuticals
Polymer C18
PC
Polymethacrylate matrix, stable
towards shrinking and swelling,
wide pH applicability
C8
OC
High coverage monomeric C8
bonding
3, 5
120, 200,
300
10, 7,
4
2-7.5
Proteins and peptides, estrogens,
general purpose phase
YMCbasic
BA
Specifically designed for
separation of basic compounds
3, 5
proprietary
8
2-7.5
Basic molecules without modifiers,
anilines, alkaloids, antidepressants
Phenyl
PH
Monomeric phenyl bonding,
endcapped
3, 5
120, 300
9, 3
2-7.5
Phenols, fullerenes, sweeteners
C4
BU
C4, fully endcapped, high
coverage monomeric bonding
3, 5
120, 200,
300
7, 5,
3
2-7.5
Biological separations, polar
compounds
Protein RP
PR
Designed to withstand exposure
to TFA, good recovery rates
5
proprietary
4
1.5-7.5
C1
TM
Trimethylsilane, excellent
hydrolytic stability
3, 5
120, 300
4, 3
2-7.5
PVA-SIL
PV
Polyvinyl alcohol bonded on silica
support, suitable for NP or RP
applications
5
120
-
2-9
Proteins, phospholipids, retinoids,
lipids
Polyamine ll
PB
Improved hydrolytic stability over
conventional amino phase,
increased lifetime, rapid
equilibration time
5
120
-
2-9
Maltooligosaccharides, tocopherols,
nucleotides, sugars
Amino
NH
Primary amino, high coverage
monomeric bonding chemistry
3, 5
120
3
2-7.5
Saccharides, nucleotides, water
soluble vitamins
Cyano
CN
Fully endcapped, also useful for
SFC applications
3, 5
120, 300
7, 2.5
2-7.5
Proteins, steroids, catechols
Diol
DL
For aqueous GPC or normalphase applications, high recovery
for biological material
5
60, 120,
200, 300
9, 7,
5, 3
2-7.5
Peptides, proteins,
maltooligosaccharides
Silica
SL
Ultra high purity, high mechanical
stability
3, 5
60, 120,
200, 300
-
2-7.5
Small organic molecules, fat soluble
vitamins, tocopherols
See page 274 for YMC preparative grade materials
Proteins, peptides
Water soluble vitamins
YMC
YMC C18 ODS-A
This phase is the traditional workhorse for most reversed-phase applications. Endcapping is exhaustive and this greatly reduces the activity of silanol
groups and minimises non-specific secondary interactions.
YMC C18 ODS-AM
YMC C18 ODS-AM is a high specification C18 material produced to meet the stringent demands for validated analytical HPLC processes. All columns
have to pass additional tests to meet the narrow quality specifications range with regards to selectivity pattern, column resolution, absolute retention and
peak symmetry.
LC Columns – YMC
YMC ‘Classical’ Phases (continued)
YMC C18 ODS-AL
This phase is a non-endcapped ODS. Non-endcapping imparts some additional secondary normal-phase adsorption interaction characteristics.
YMC ODS-AQ
ODS-AQ differs from traditional C18 phases in that after initial octadecylsilyl bonding, the material is treated to produce a surface that can be wetted with
polar eluents. In highly aqueous eluents ODS-AQ gives longer retention times for polar compounds than conventionally endcapped ODS packings.
YMC Polymer C18
Polymer C18 is a reversed-phase material which provides a broad range of solvent choices over a pH range of 2 – 13, due to its resistance to shrinking
and swelling. It consists of a highly cross-linked spherical hydrophilic methacrylate polymer which is functionalised with C18 groups. Polymer C18
provides similar, but not identical, selectivity to standard silica-based ODS materials. It has slightly greater retention for aromatic compounds than even
highly retentive ODS columns. It is significantly less retentive than styrene/divinylbenzene based HPLC columns.
YMCbasic
YMCbasic is an early base deactivated phase, widely used for the analysis of pharmaceutical and other basic compounds. The phase consists of
monomerically bonded alkyl chains with different chain lengths of C8 and smaller, leading to high surface coverage. Its selectivity is similar to that
expected for conventionally endcapped C8 reversed-phase media.
YMC J’sphere
In the YMC J’sphere series of phases, instead of varying the alkyl group chain length in order to produce phases of different polarity, the surface coverage
of C18 is varied to produce three phases of graduated hydrophobicity and hydrogen bonding characteristics. The stepwise decrease of hydrophobicity in
the J’sphere ODS-H80, M80 and L80 series is accompanied by a corresponding increase in the H-bonding capacity.
YMC Carotenoid
The YMC Carotenoid (C30) phase is very lipophilic with exceptional selectivity towards isomeric structures. The C30 ligands provide sufficient phase
thickness to enhance interaction with long chained molecules. Geometrical and positional isomers of conjugated double bond systems can be recognised
and resolved. The phase is particularly suited for the separation of carotenoids (see Figure 7). Other potential applications include the separation of
prostaglandins and leukotrienes.
1. Lutein
2. Zeaxanthin
3. ß-Cryptoxanthin
4. 13-cis ß-Carotene
5. α-Carotene
6. trans ß-Carotene
7. 9-cis ß-Carotene
8. Lycopene
Column: YMC Carotenoid (5µm, 250 x 4.6mm)
Eluent: A: CH3OH - MTBE - H2O (81:15:4)
B: CH3OH - MTBE - H2O (6:90:4)
Gradient A to B in 90mins
Flow rate: 1ml/min
Temperature: Ambient
Detection: UV, 450nm
8
6
1
3
2
5
4
0
10
20
30
40
7
50
60
70
80 Mins
Figure 7. Extract of SRM 2383, NIST food standard
Hichrom Limited
www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660
271
LC Columns – YMC
YMC
Ordering Information - YMC ‘Classical’ 120Å Phases
YMC2 Phase
3μm Particle Size
50 x 3.0
100 x 3.0
150 x 3.0
100 x 4.6
150 x 4.6
-
ODS-AQ
ODS-A
AA12S03-0503QT AA12S03-1003QT AA12S03-1503QT AA12S03-0546WT AA12S03-1046WT AA12S03-1546WT AA12S03-0104GC
£314
£347
£388
£347
£380
£405
£273
4μm Particle Size
50 x 3.0
150 x 3.0
250 x 3.0
J’sphere ODS-L805
JL08S04-0503QT
£355
JL08S04-1503QT
£455
JL08S04-2503QT
£537
J’sphere ODS-M805
JM08S04-0503QT JM08S04-1503QT JM08S04-2503QT JM08S04-0546WT JM08S04-1546WT JM08S04-2546WT JM08S04-0104GC
£355
£455
£537
£372
£496
£521
£273
J’sphere ODS-H805
JH08S04-0503QT
£355
JH08S04-1503QT
£455
50 x 4.6
150 x 4.6
250 x 4.6
-
50 x 3.0
150 x 3.0
JL08S04-0546WT JL08S04-1546WT JL08S04-2546WT
£372
£496
£521
JL08S04-0104GC
£273
JH08S04-2503QT JH08S04-0546WT JH08S04-1546WT JH08S04-2546WT JH08S04-0104GC
£537
£372
£496
£521
£273
250 x 3.0
50 x 4.6
150 x 4.6
250 x 4.6
-
ODS-AQ
AQ12S05-0503QT AQ12S05-1503QT AQ12S05-2503QT AQ12S05-0546WT AQ12S05-1546WT AQ12S05-2546WT AQ12S05-0104GC
£314
£380
£413
£339
£405
£430
£273
ODS-A
AA12S05-0503QT AA12S05-1503QT AA12S05-2503QT AA12S05-0546WT AA12S05-1546WT AA12S05-2546WT AA12S05-0104GC
£314
£380
£405
£306
£372
£405
£273
ODS-AM
AM12S05-0503QT AM12S05-1503QT AM12S05-2503QT AM12S05-0546WT AM12S05-1546WT AM12S05-2546WT AM12S05-0104GC
£314
£380
£413
£339
£405
£430
£273
ODS-AL
AL12S05-0503QT
£339
AL12S05-1503QT
£380
50 x 3.0
150 x 3.0
6μm Particle Size
Polymer C18
AL12S05-2503QT AL12S05-0546WT AL12S05-1546WT AL12S05-2546WT AL12S05-0104GC
£430
£339
£413
£446
£273
250 x 3.0
50 x 4.6
150 x 4.6
250 x 4.6
-
PC99S06-0503QT PC99S06-1503QT PC99S06-2503QT PC99S06-0546WT PC99S06-1546WT PC99S06-2546WT PC99S06-0104GC
£372
£496
£537
£413
£545
£521
£273
Column Dimensions4 (mm)
YMC2, 3 5μm Phase
100 x 4.6
Guard Cartridges1
150 x 4.6
250 x 4.6
(5/pk, 10 x 4mm)
Carotenoid (C30)
CT99S05-1046QT £413
CT99S05-1546QT £496
CT99S05-2546QT £554
CT99S05-0104GC £273
C8
OC12S05-1046WT £388
OC12S05-1546WT £413
OC12S05-2546WT £446
OC12S05-0104GC £273
Basic
BA99S05-1046WT £413
BA99S05-1546WT £446
BA99S05-2546WT £504
BA99S05-0104GC £273
C4
BU12S05-1046WT £388
BU12S05-1546WT £413
BU12S05-2546WT £446
BU12S05-0104GC £273
TMS
TM12S05-1046WT £405
TM12S05-1546WT £405
TM12S05-2546WT £446
TM12S05-0104GC £273
Phenyl
PH12S05-1046WT £405
PH12S05-1546WT £405
PH12S05-2546WT £446
PH12S05-0104GC £273
Protein RP
PR99S05-1046WT £521
PR99S05-1546WT £521
PR99S05-2546WT £562
PR99S05-0104GC £273
SIL
SL12S05-1046WT £347
SL12S05-1546WT £372
SL12S05-2546WT £405
SL12S05-0104GC £273
PVA-SIL
PV12S05-1046WT £504
PV12S05-1546WT £479
PV12S05-2546WT £554
PV12S05-0104GC £273
Polyamine ll
PB12S05-1046WT £413
PB12S05-1546WT £446
PB12S05-2546WT £504
PB12S05-0104GC £273
NH2
NH12S05-1046WT £405
NH12S05-1546WT £405
NH12S05-2546WT £446
NH12S05-0104GC £273
CN
CN12S05-1046WT £405
CN12S05-1546WT £405
CN12S05-2546WT £446
CN12S05-0104GC £273
Diol
-
-
DL12S05-2546WT £504
-
Use with universal holder XPGCH-Q1 (£132) or direct connect holder XPGCS-Q1 (£132). 3mm i.d. guard cartridge also available
2
60Å, 200Å or 300Å pore sizes also available for selected materials
3
Available in 3μm particle size for selected materials 1
For free technical advice and support email [email protected]
(5/pk, 10 x 4mm)
50 x 4.6
AQ12S03-0503QT AQ12S03-1003QT AQ12S03-1503QT AQ12S03-0546WT AQ12S03-1046WT AQ12S03-1546WT AQ12S03-0104GC
£339
£355
£405
£339
£405
£413
£273
5μm Particle Size
272
Guard Cartridges1
Column Dimensions4 (mm)
Other dimensions (eg 2.1 and 1.0mm i.d.) also available
5
80Å
4
YMC Guard Cartridge Holders
For 4.6mm i.d. analytical columns, the universal holder XPGCH-Q1 is required for 10mm length guard cartridges and XPGCH-Q2 for 20mm length guard
cartridges (see figure 8). For 1.0 to 4.0mm i.d. guard cartridges, the customer can choose between this universal holder or an integral (direct connect)
holder XPGCS-Q1 for 10mm length cartridges and XPGCS-Q2 for 20mm length guard cartridges (see figure 9). The integral holder replaces the inlet
endfitting on the column with a hand-tightened holder which allows for a very low dead volume connection.
Figure 8. Universal holder XPGCH-Q1 or XPGCH-Q2
Figure 9. Integral holder XPGCS-Q1 or XPGCS-Q2
YMC
LC Columns – YMC
YMC-BioPro
• Porous and non-porous hydrophilic polymer
• Ion-exchange phases
• Excellent binding capacity and recovery
• High resolution
YMC-BioPro is a series of porous and non-porous ion-exchange phases based on a hydrophilic polymer with low non-specific adsorption. The phases are
designed for the analysis and purification of peptides, proteins, nucleic acids and other biomolecules. They also show higher binding capacity and higher
recovery of biomolecules compared to conventional ion-exchange columns. The completely spherical, monodisperse beads, together with optimal packing
technology, provide high efficiency and symmetrical peak shapes.
YMC-BioPro Phases
YMC Phase
QA
SP
QA-F
SP-F
Pore Size (Å)
1000
1000
Non-porous
Non-porous
5
5
5
5
-CH2N (CH3)3
Cl-
-CH2CH2CH2SO3
-CH2N (CH3)3
Cl-
-CH2CH2CH2SO3
Particle Size (μm)
Charged Group
+
Counter Ion
pH Range
Dynamic Binding Capacity
+
Na+
Na+
2-12
2-12
2-12
2-12
110-150mg BSA/ml resin
70-100mg human IgG/ml resin
12-20mg BSA/ml resin
10-18mg human IgG/ml resin
The porous YMC-BioPro phases show high dynamic binding capacity and excellent recovery, making them useful for semi-preparative separations of
proteins and antibodies. Figure 10 shows a loading study for proteins on a YMC-BioPro QA column. Figure 11 shows the high resolution separation of
DNA fragments achievable with a YMC-BioPro QA-F column.
Column:YMC-BioPro QA (5μm, 50 x 4.6mm)
Eluent: A. 20mM Tris-HCl (pH 8.1)
B. 20mM Tris-HCl (pH 8.1) containing 0.5M NaCl
10-80% B over 30 minutes
Flow rate: 0.5ml/min
1
Temperature: 25°C
Detection: UV, 280nm
1. Ovalbumin
2. Trypsin inhibitor (soybean)
Column: YMC-BioPro QA-F (5μm, 100 x 4.6mm)
Eluent: A. 20mM Tris-HCl (pH 8.1) containing 0.5M NaCl
B. 20mM Tris-HCl (pH 8.1) containing 1.0M NaCl
40-100% B over 30 minutes
Flow rate: 0.5ml/min
Temperature: 25°C
Detection: UV, 260nm
1kb DNA ladder (75-12,216 bp)
2
500µg
250µg
100µg
50µg
0
10
20
Mins
30
0
5
10
15
Mins
20
Figure 11. DNA fragments analysed on YMC-BioPro QA-F
Figure 10. Loading study for YMC-BioPro QA
Ordering Information1
Column Dimensions (mm)
SP
QA-F
SP-F
30 x 4.6
-
-
QF00S05-0346WP £760
SF00S05-0346WP £760
50 x 4.6
QAA0S05-0546WP £926
SPA0S05-0546WP £926
£893
SF00S05-0546WP £893
100 x 4.6
QAA0S05-1046WP £992
-
QF00S05-0546WP
QF00S05-1046WP £1,017
SF00S05-1046WP £1,017
Preparative grade YMC-BioPro also available as bulk material
www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660
Hichrom Limited
1
YMC-BioPro Phase
QA
273
LC Columns – YMC
YMC
YMC Capillary Columns
YMC manufacture a range of LC-MS compatible nano and capillary columns of 75, 100, 300 and 500μm i.d. and lengths of 50, 75, 100 and 150mm.
For highly contaminated samples, the use of pre-columns is recommended. These connect to the capillary column with a pre-column coupler which is
included. Pre-columns can also be used as trapping columns.
Figure 12. Capillary column with pre-column attached
YMC Chiral Columns
YMC manufacture a range of chiral columns, based on different ligand types, suitable for a wide range of chiral separations. See also page 5 for the new
YMC CHIRAL polysaccharide series of columns.
YMC Chiral Phases
YMC Phase
Ligand
Particle Size (μm)
Pore Size (Å)
Recommended pH Range
1-Naphthylethylamine
5
300
2 - 6.5 (RP)
Bromide derivative of cyclodextrin (α, β or γ)
5
120
3.5 - 6.5
Various – 17 different types
5
120
-
Chiral NEA (R) or (S)
CD BR (α, β or γ)
Sumichiral OA Series
YMC Chiral NEA (R) and (S)
YMC Chiral NEA consists of 1-naphthylethylamine bonded to wide pore spherical silica. (R) and (S) versions are available for elution order reversal. YMC
Chiral NEA columns can be used in normal-phase and reversed-phase modes.
YMC Chiral CD BR
YMC Chiral CD BR phases consist of bromide derivatives of α-, β- or γ-cyclodextrin,
in which the primary hydroxyl groups at carbon 6 are substituted for bromine. These
phases are used in reversed-phase mode to separate a wide range of polar,
water-soluble compounds and positional isomers of substituted aromatic compounds.
YMC Sumichiral OA
Seventeen different phases of Sumichiral OA columns are available. These are
based on different types of covalent linkage, including amide, urea, ligand exchange
type, cyclodextrin type and crown ether phases.
Please contact Hichrom for ordering information for YMC chiral columns.
YMC Chiral b-CD BR
For free technical advice and support email [email protected]
YMC SFC Columns
274
In addition to its application in enantiomer purification, SFC is gaining importance in the separation of achiral compounds. YMC offer six different
selectivity phases for achiral SFC:
• Diol
• Amino • Cyano • PVA-Sil
• Polyamine ll
• Silica
For preparative SFC, YMC has developed special column hardware in order to meet the highest demands in quality, safety and reliability.
Please contact Hichrom for further information on YMC SFC columns.
YMC Preparative Separations
YMC preparative columns, packed with a variety of YMC materials, are available in a wide range of column sizes to accommodate virtually any preparative
separation and direct scale-up from analytical and semi-preparative separations. Standard column dimensions range from 50 to 200mm i.d. and 250 to
1000mm in length. Matching YMC preparative guard columns are available. Please contact Hichrom for further information.
YMC
• Purification and isolation of maximum sample quantity
• Savings in time and costs
• Excellent durability and efficiency
YMC-Actus semi-preparative/preparative columns use modified hardware and packing technology. These columns are specifically designed for fast
semi-preparative/preparative chromatography and are available in a range of dimensions from 50 x 20mm i.d. to 250 x 50mm i.d. A uniform packing
density results in a longer lifetime than conventional semi-preparative/preparative columns. YMC-Actus columns provide reliable results, even after
exposure to severe, rapid gradient conditions and multiple injections.
LC Columns – YMC
YMC-Actus for Fast Semi-preparative/Preparative Separations
Figure 13 illustrates the excellent durability and efficiency of the purification of glycyrrhizic acid from a herbal medicine achieved under fast gradient
conditions at high flow rate.
Analysis
YMC-Pack ODS-AQ, 50 x 4.6 mm i.d., 5µm
3ml/min, 10µl injection
Glycyrrhizic acid
Glycyrrhizic acid
0
5
10 Mins
Purification
YMC-Actus ODS-AQ, 50 x 20 mm i.d., 5µm
60ml/min, 500µl injection
After purification
Glycyrrhizic acid
99.5%
0
0
5
5
10
10 Mins
Mins
Eluent: A: Water – acetic acid (99:1) B: Methanol – acetic acid (99:1)
Gradient: 20% B (0 – 2mins), 20-45% B (2 – 7 mins), 45% B (7 – 10mins)
Temperature: Ambient
Detection: UV, 260nm
Sample: Water – methanol – acetic acid extract of commercially available herbal medicine (0.1g/ml)
Figure 13. Purification of glycyrrhizic acid from herbal medicine
Ordering Information1 (5μm, 50 x 20mm i.d. Columns)
Phase
1
Pore Size (Å)
Part Number
Price
Phase
Pore Size (Å)
Part Number
Price
Triart C18
120
TA12S05-0520WX
£959
ProC18RS
80
RS08S05-0520WX
£959
Triart C8
120
TO12S05-0520WX
£959
ODS-A
120
AA12S05-0520WX
£959
ProC18
120
AS12S05-0520WX
£959
ODS-AQ
120
AQ12S05-0520WX
£901
Hydrosphere C18
120
HS12S05-0520WX
£959
ProC8
120
OS12S05-0520WX
£959
Other phases and dimensions available. Please contact us for details.
Bulk Material
YMC supply a wide range of bulk media for scale-up to preparative dimensions and beyond. These include materials for reversed-phase, normal-phase,
SEC, ion-exchange and chiral. A selection of media is listed below. Please contact us for media not listed.
HG stands for ‘High Grade’
1
Particle Size (μm)
10, 15, 20, 50
10
10, 15, 20, 50
10, 15, 20
10, 30, 75
10, 20, 50
10
Pore Size1 (Å)
120, 200
120
80, 120, 200, 300
120, 200, 300
1000
120
300
Not all combinations of particle size and pore size are available
www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660
Hichrom Limited
Material
Triart Prep C18, C8
ProC18
ODS-A-HG, ODS-AQ-HG, C8-HG, C4-HG, NH2-HG, CN-HG, Diol-HG, SIL-HG, Ph-HG, TMS-HG
Diol
BioPro QA, SP
Chiral Prep CD ST and CD PM
Chiral Prep NEA
275