Retrospective Study of Some Tumors at the Genital Tract of Dogs

Awad Rizk et al., J Vet Sci Med Diagn 2015, 4:2
http://dx.doi.org/10.4172/2325-9590.1000158
Journal of Veterinary
Science & Medical
Diagnosis
Research Article
A SCITECHNOL JOURNAL
Retrospective Study of Some
Tumors at the Genital Tract of
Dogs
1*A.Z.
Rizk, 2M.F. Hamed and 1A.E. Zaghloul
¹Department of Surgery, Anesthesiology and Radiology, Mansoura University,
35516 Mansoura, Egypt
²Department
of Pathology, Faculty of Veterinary Medicine, Mansoura University,
35516 Mansoura, Egypt
*Corresponding author: Awad Rizk, Surgery, Anesthesiology and Radiology
Department, Faculty of Veterinary Medicine, Mansoura University, 35516
Mansoura, Egypt; Tel: 00201099513339; E-mail: [email protected]
Rec date: 26 Aug, 2014 Acc date: Jan 13, 2015 Pub date: Jan 20, 2015
Abstract
The objective of the present study was to through light on
clinical, histopathological and immunohistochemical (IHC),
differential diagnosis as well as evaluation of surgical
interventions and outcome of canine external genital tumors.
Twenty one cases of canine genital neoplasms (12 vulvar and
vaginal tumors and 9 penile and preputial tumors) were
recorded. History, clinical, histopathological and IHC
examination as well as abdominal ultrasonographic evaluation
were performed. The neoplasm's were vaginal leiomyosarcoma
[n= 3], perivulvar lipoma [n=1], vaginal cystic polyp [n=1], vulvovaginal adenocarcinoma [n= 2], vaginal transmissible venereal
tumour (TVT) [n= 5] as well as penile [n= 3] and preputial [n= 6]
TVT, respectively. Local excision with/or without episiotomy
was satisfactory with no recurrence of the tumor for about 2
years post-surgery. It was concluded that, surgical excision
alone or combined with an episiotomy was successful with no
recurrence of the tumour. TVT is rarely metastasis and this
really indicated by intense lymphocytic infiltration in dermis of
prepuce and vaginal submucosa of our cases. There are no
detailed reports of microscopical feature of vaginal cystic polyp
in canine.
Keywords: Cystic polyp; TVT; Leiomyosarcoma; Perivulvar lipoma;
Vagina; Prepuce; Dog
Introduction
Vaginal and vulvar tumors are the second most common canine
female reproductive tumor after those of the mammary gland. They
constitute 2.4 - 3% of canine neoplasia [1]; of this 85-90% occur in the
vagina and vulva. Most benign tumors are therefore amenable to
management by local resection via episiotomy combined with
ovariohysterectomy [2].
Leiomyoma, fibroma, and TVT account for most of the canine
vulvar and vaginal neoplasms [1]. Other canine vaginal tumors such as
adenocarcinoma, epidermoid carcinoma, fibrous histiocytoma,
lipoma, myxoma/ myxofibroma, melanoma, mast cell sarcoma,
osteosarcoma, papilloma, polyp, hemangiosarcoma, leiomyosarcoma
and squamous cell carcinoma are rarely reported [3,4,5].
Vulvar lipoma tends to occur in younger dogs in the age 1-8 years
[1,6]. It is not invasive, not aggressive and produces no metastasis to
tissues and/or distant organs. Its location in vulvar or peri-vulvar is
quite rare and cause local irritation [7, 8].
Vaginal polyps are pedunculated single or multiple structures often
arise from the ventral floor of the vagina [9]. Polyps develop from
reoccurring edema and fibrosis of proestrus and oestrus in bitches,
with the largest developing on the ventral floor cranial to the urethral
opening. They can eventually protrude from the vulva where they
become ulcerated [9].
Cutaneous apocrine adenocarcinomas are well recognized in dogs
and cats [10,11]; they are locally infiltrative with a low metastatic rate.
They could occur in the peri-vulval region and would be
indistinguishable from primary vulval adenocarcinoma [12, 13, 14].
Canine TVT are an unusual neoplasm occurs in both males and
females [15]. Mostly, TVT are solitary or multiple tumors usually
appear as cauliflower like growths or as nodules on a stalk. The
growths may be multinodular and/or ulcerated [16]. In females, TVT
develop in the vagina and on the vulva. In the male dog, the tumor is
usually located on the caudal part of the penis and occasionally on the
prepuce [17, 18]. TVT is detected in the extragenital regions in dogs at
the skin of the perineum, face, side of the thorax, mouth, nasal cavity,
limbs and conjunctiva [19,20,21].
The ultimate goal of treatment of the tumor is complete cure, which
may be achieved by surgical excision, radiotherapy, immunotherapy
and chemotherapy [22]. Radiotherapy has been reported to be effective
against TVT, but necessitates chemical immobilization of the dog
during the radiotherapy with specialized personnel and equipment
[23]. Vincristine sulphate is an effective chemotherapeutic agent in the
treatment of TVT despite its many side-effects. Small masses, which
are not of typical TVT appearance, can still persist even after a
combination therapy with vincristine and doxorubicin. These masses
should be removed with surgery [23].
The aim of the present study was to through light on clinical,
histopathological and IHC, differential diagnosis as well as evaluation
of surgical intervention and outcome of canine external genital
tumors.
Materials and Methods
In recent years, a total number of 21 intact dogs (4- 12 years old) of
different breeds were presented with a vulvo-vaginal masses (n= 12)
and penile and preputial masses (n= 9) (Table 1). All cases were
admitted to Mansoura Veterinary Teachings Hospital of the Faculty of
Veterinary medicine, Mansoura University, Mansoura, Egypt.
Diagnosis was based up on case history, clinical examination of each
overgrowth (includes location, size and shape), abdominal
ultrasonography, histopathological, and IHC evaluation.
Cases Descriptions
Case A (3 bitches)
Two German shepherd and one Doberman bitches were referred
for evaluation of a mass protruding from the vulvo-vaginal ostium.
The finding of clinical examination showed that the mass was round,
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Citation:
Rizk AZ, Hamed MF, Zaghloul AE (2015) Retrospective Study of Some Tumors at the Genital Tract of Dogs. J Vet Sci Med Diagn 4:2.
doi:http://dx.doi.org/10.4172/2325-9590.1000158
hard, necrotic and protruded from vulva. One of these cases associated
with partial vaginal prolapse.
Case
Sex
No
Age
Weight
(yr)
(Kg)
Breed
Tumor
Location
Treatment
Recurrence
type
Follow
2nd treatment
Follow-up
-up
1
F
8
35
German
shepherd
LMS
Vagina
Loc+Epi
No
22 mo
-
-
2
F
11
30
Doberman
LMS
Vagina
Loc+Epi
Yes
3 mo
Yes
18 mo
3
F
5
5
Lolo
LIP
Peri-vulvar
Loc
No
15 mo
-
-
4
F
6
25
German
shepherd
VCP
Vagina
Loc
No
20 mo
-
-
5
F
6
52
Great dane
ADC
Vagina
Loc+Epi
No
18 mo
-
-
6
F
9
75
Great dane
ADC
Vulvovaginal
Loc+Epi
No
22 mo
-
-
7
M
5
45
Great dane
TVT
Prepuce
Loc
Yes
6 mo
Yes
24 mo
8
M
6
31
German
shepherd
TVT
Penis
Loc
No
24mo
-
-
9
F
7
28
German
shepherd
TVT
Vagina
Loc+Epi
No
18 mo
-
-
10
F
10
26
German
shepherd
LMS
Vagina
Loc
No
12 mo
-
-
11
F
8
27
Doberman
TVT
Vagina
Loc
No
15mo
-
-
12
M
8
33
German
shepherd
TVT
Penis
Loc
No
12 mo
-
-
13
M
9
68
Great dane
TVT
Prepuce
Loc
No
15mo
-
-
14
M
6
49
Great dane
TVT
Penis
Loc
No
18mo
-
-
15
M
9
33
German
shepherd
TVT
Penis
Loc
No
24mo
-
-
16
M
10
55
Great dane
TVT
Penis
Loc
No
14mo
-
-
17
M
12
62
Great dane
TVT
Penis
Loc
No
24mo
-
-
18
M
8
22
German
shepherd
TVT
Prepuce
Loc
No
20mo
-
-
19
F
4
22
German
shepherd
TVT
Vagina
Loc
No
12 mo
-
-
20
F
8
30
German
shepherd
TVT
Vagina
Loc
Yes
12mo
Yes
22mo
21
F
9
32
German
shepherd
TVT
Vagina
Loc+Epi
No
20mo
-
-
Table 1: Showing a patient data including animal species, age, tumour types, treatment, recurrence and follow-up of vulvar, vaginal, penile and
preputial neoplasms in dogs F= female M= male dog Loc= local excision Epi= episiotomy LMS= leiomyosarcoma VCP= vaginal cystic polyp
LIP= lipoma ADC= adenocarcinoma TVT= transmissible venereal tumor
Case B (one bitch)
Case C (one bitch)
A Lolo bitch was presented with a large ulcerated, round, soft and
white looks like perineum mass. Based on history, the mass gradually
increased in size over time and sufficiently close the vulva.
A German shepherd bitch was presented with the complaint of a
moderate abnormal tennis ball sized mass, red, smooth and coneshaped protruding between vulvar lips. It was originated from vaginal
wall.
Volume 4 • Issue 2 • 1000158
• Page 2 of 7 •
Citation:
Rizk AZ, Hamed MF, Zaghloul AE (2015) Retrospective Study of Some Tumors at the Genital Tract of Dogs. J Vet Sci Med Diagn 4:2.
doi:http://dx.doi.org/10.4172/2325-9590.1000158
Case D (2 bitches)
Two Great Dane bitches were referred to our clinic with a history of
mass popping out off the vulva since about two months, with a
difficulty in urination and defecation. Physical examination revealed a
cauliflower growth with a stock like structure originating from the
vaginal mucous membrane.
Case E (5 bitches)
Four German shepherd and one Doberman bitches were admitted
with a cauliflower like mass protruded from the vulva (Figure 7A).
the secondary biotin-labeled antibody was incubated for 30 min at
room temperature. The streptavidin labeled streptavidin-biotin
amplification method (cat. NO. KO672, Dako universal LSAB-2
system, USA) was carried out for 30 minutes followed by
diaminobenzidine chromagen. The slides were counterstained with
hematoxylin.
Follow-up: Follow-up information was obtained by visit and
telephone inquiries. Owners were questioned about the dog's general
health status, recurrence of masses, complications from the surgical
procedure, and the ability of the operated animals to urinate freely.
Case F (9 male dogs)
Results
Five Great Dane and four German shepherd male dogs were
admitted. Chief complain of the owner was to drops a bloody
discharge from the preputial sac just after urination and after prolong
siting by the animal. Each animal was breed with 3-4 females in last 2
months. Physical examination after sedation of each animal revealed a
pinkish grey cauliflower like, irregular, friable multiple nodular mass
located on the prepuce and/ or at base of the penis in a circular
fashion. Extragenital lesions on the face, chest and hind limbs were
recorded in one of these dogs.
In the present study, 21 dogs (12 bitches and 9 male dogs) showed
various types of vulvo-vaginal, penile and preputial neoplasms (Table
1). The diagnosed neoplasms were vaginal leiomyosarcoma (n= 3),
peri-vulvar lipoma (n= 1), vaginal cystic polyp (n= 1), vulvo- vaginal
adenocarcinoma (n= 2) and vaginal (n= 5), penile (n= 3) and preputial
(n= 6) TVT. Local excision with or without episiotomy was successful
in all cases with no recurrence of the tumor until about 2 year post
surgery except three cases (first leiomyosarcoma, second preputial
TVT and the third is vaginal TVT) showed recurrence after 3, 6 and 12
months respectively. Surgical excision was performed in the three
cases for a second time with successful recovery without recurrence.
Two cases of leiomyosarcoma and a case of adenocarcinoma get
pregnant after surgery.
Abdominal ultrasonographic evaluation: Ultrasonography of the
abdomen and mammary gland was performed for detection of tumor
metastasis using Shenzhen Mindary (DP-220vet, China) with 5, 7.5 or
10 MHz linear transducer.
Surgical interventions: All dogs were premedicated with atropine
sulphate in a dose of 0.04 mg/kg body weight (BW) intramuscularly
(i.m.) and sedated with xylazine hydrochloride (xylaject, ADWIA,
Egypt) in a dose of 1.0 mg/kg BW i.m. Lumbosacral epidural analgesia
was performed in 5 dogs using lidocaine 2% (Debocaine, El-Debeaky,
Egypt) in a dose of 1ml / 5kg BW. General anesthesia were induced
and maintained with the use of thiopental sodium (Thiopental 500 mg
vial, EPICO, Egypt) to effect in the rest of cases. Bladder
catheterization using a foley catheter (8-10 French) was performed in
all bitches.
All cases were subjected to local excision of the mass, while in deep
seated cases (2 leiomyosarcoma, 2 adenocarcinoma and 2 vaginal
TVT) an episiotomy was performed at one o'clock position relative to
the dorsal commissure of the vulva (Figure 4B). The mass was easily
visualized and it was noted that the vagina was extremely dilated. The
growth was resected out. The episiotomy incision was closed by using
2/0 chromic catgut with simple interrupted suture pattern. Skin
incision was closed routinely. After owners' consultations,
ovariohysterctomy was performed in four bitches. In the case of
vaginal cystic polyp, the mass was crushed and legated with an
absorbable suture material before surgical excision.
Histopathological examination: The excised masses were fixed in
10% neutral buffered formalin, and sent to the pathology laboratory at
our University for histopathological examination. Paraffin sections
with 5-mm thickness, were prepared routinely and stained with
hematoxylin and eosin (H&E) according to [24].
Immunohistochemical (IHC) examination: In order to confirm
lieomyosarcoma and cystic polyp, IHC examination were performed
using a mouse monoclonal antibody of alpha- smooth muscle actin
and desmin respectively. After paraffin removal and hydration, the
slides were immersed in 10 mM citrate buffer pH 6, for 15 min for
antigen retrieval. The antibodies were incubated overnight at 4°C, and
Volume 4 • Issue 2 • 1000158
Case A (leiomyosarcoma): Clinically, the mass appeared firm,
white-brown in color and associated with partial vaginal prolapse in
one case (Figure 1A). Grossly, on cut section it appeared with multiple
nodularity of grayish white to brown in color, firm in consistency and
approximately 6 x 4.5 x 4 cm in size (Figure 1B).
Histologically, large number of hyper cellular pleomorphic
malignant cells which is haphazard in arrangement with tendency to
form fasicles. Their nuclei are vesicular and densely basophilic with
prominent nucleoli. Some cells retain their spiral shape nuclei and
arrangement in bundles (Figure 1D). The IHC staining was uniform in
all evaluated sections. Malignant cell stained brown by alpha smooth
muscle actin (Figure 1E).
Recovery without recurrence was obtained in two cases in about 2
years post-surgery (Figure 1C), while the third case showed regrowth
of the mass 3 months post-surgery but less in size. It showed a
successful recovery after the second surgery.
Case B (Peri-vulvar lipoma): The peri-vulvar mass appeared
ulcerated, soft and looks like perineal mass (Figure 2A&2B). On the
cut section, it appeared as well capsulated mass with whitish color and
fatty in consistency (Figure 2C). Microscopic examination of the
tumor revealed well differentiated lipocytes without evidence of
malignancy (Figure 2D).
Case C (Vaginal cystic polyp): Grossely, the mass appeared red in
color and soft in consistency protruding from the vulva (Figure
3A).The histological examination demonstrated cystic dilatation
which filled with serous fluid with well differentiated fibro collagenous
stroma which has tendency to form whorl shape (Figure 3B). IHC
revealed fibrous tissue stained brown positive for desmin for
differention from leiomyosarcoma (Figure 3C).
Case D (Adenocarcinoma): A cauliflower-like mass showed
multiple nodularity, firm in consistency and bulging the vulva out
• Page 3 of 7 •
Citation:
Rizk AZ, Hamed MF, Zaghloul AE (2015) Retrospective Study of Some Tumors at the Genital Tract of Dogs. J Vet Sci Med Diagn 4:2.
doi:http://dx.doi.org/10.4172/2325-9590.1000158
(Figure 4A&4C). Histologically, the mass showed cellular atypia,
malignant cells has dense basophilic vacuolated nucleus which has
tendency to form acini and increase rate of necrosis (Figure 4D).
Cases E&F (TVT): It appeared as a cauliflower- like mass (Figure
6A) and (Figure 5A& 5B) at the base of the penis (n= 3), prepuce (n=
6) and vagina (n=5) (Figure 7A). Extra-genital TVT case appeared as a
nodular, ulcerated and of variable sizes lesion on the face, thorax and
hind limb (Figure 5C). The mean average of each mass measured
about 10x 5x4 cm after surgical excision (Figure 6B).
Microscopically, in male dogs, large pleomorphic round cells, their
nucleus is large vesicular with prominent nuclei. Anaplastic cells
arranged radially around blood capillaries (Figure 6D). A large round
cell and numerous blood capillaries with lymphocytic infiltration in
dermis of the prepuce (Figure 5D). In bitch, sheets of anaplastic cells
with little fibrous stroma beside vaginal mucosa infiltrated with
lymphocytes (Figure 7B). Sheets of small round anaplastic cells with
prominent nucleoli arranged radially around blood vessels were
observed (Figure 7C). Penile and preputial masses as well as vaginal
mass show successful recovery and outcome after surgical excision
(Figure 6C).
Figure 1: (A) A large firm mass protruding from vagina with partial
vaginal prolapse in a 7 years old German shepherd bitch (B) The
cut section of the mass showed multiple nodularity of grayish white
to brown in color and firm in consistency (C) Retraction of vagina
after removal of the mass and vulva return to normal shape and
position (D) Hyper cellularity of pleomorphic malignant cells
haphazard in arrangement with tendency to form whorls with
densely basophilic and vesicular nuclei with prominent nucleoli
and some cells retain their spiral shape nuclei and arrangement in
bundles (E) H&E original magnification x 400. Malignant cell
stained brown by alpha smooth muscle actin (arrow), (DAB
immunostain and HE counter stain)
Volume 4 • Issue 2 • 1000158
Figure 2: A 5 years old Lolo bitch showed a well capsulated fatty
mass with ulcerated surface at perivulvar region (arrow, A&B). On
cut section the mass appeared whitish in color and fatty in
consistency (C). Micrograph of perivulvarlipoma in bitch showed a
well differentiated lipocytes without evidence of malignancy (D).
H&E original magnification x 400
Figure 3: A 6 years old German shepherd bitch with red color mass
protruding from the vulva (A). Cystic dilatation of bartholin gland
(arrow) with well differentiated fibro collagenous stroma with
tendency to form whorl shape (B). Fibrous tissue stained brown
positive for desmin. (DAB immunostain, HE counterstain, 10x)(C)
• Page 4 of 7 •
Citation:
Rizk AZ, Hamed MF, Zaghloul AE (2015) Retrospective Study of Some Tumors at the Genital Tract of Dogs. J Vet Sci Med Diagn 4:2.
doi:http://dx.doi.org/10.4172/2325-9590.1000158
Figure 4: A 9 years old Great Dane bitch showed a popping out of
the vulva (A). An episiotomy incision was performed at one o'clock
position (arrow) (B). The mass appeared cauliflower-like growth
with multiple nodularity and firm in consistency (C). Hyper
cellularity with dense basophilic vacuolated nucleus with tendency
to form acini (arrow) and increase rate of necrosis (D)
Figure 5: A 7 years old Great Dane male dog showed a cauliflower
preputial TVT lesion (A). Another dog showed a nodular TVT
lesion on the caudal part of the penis (B). A third dog showed a
TVT lesion on the face (C).A large uniform round cells and
numerous blood capillaries with lymphocytic infiltration in dermis
of the prepuce (D)
Figure 6: A 5 years old Great Dane male dog showed a cauliflowerlike growth (arrow) located on the penis and prepuce (A) The mass
after surgical excision (B) Slight inflammation of the penis and
complete healing of the wound 2 weeks post -surgery (C) A large
round cells with vesicular nuclei and prominent nucleoli, arranged
in radial pattern around blood vessels with high mitotic rate of
division (D)
Figure 7:A cauliflower like TVT mass protruding from the vulva in
a 4 year old German shepherd bitch (A).Sheets of anaplastic cells
with little fibrous stroma beside vaginal mucosa infiltrated with
lymphocytes (HE, 4x)(B).Sheets of small round anaplastic cells with
prominent nucleoli arranged radially around blood vessels (HE,
10x) (C).
Discussion
Effective treatment methods for benign tumors of vagina include
surgical excision, cryosurgery, immunotherapy and chemotherapy.
Although, chemotherapy, radiation therapy or diathermy alone or in
combination with surgical excision can also be tried but cryotherapy is
found superior due to its analgesic and hemostatic effects, apart from
Volume 4 • Issue 2 • 1000158
• Page 5 of 7 •
Citation:
Rizk AZ, Hamed MF, Zaghloul AE (2015) Retrospective Study of Some Tumors at the Genital Tract of Dogs. J Vet Sci Med Diagn 4:2.
doi:http://dx.doi.org/10.4172/2325-9590.1000158
being simple, quick and economical, surgical removal of the tumors is
commonly practiced [25]. Surgical excision is the most successful in
animals with few, small, circumscribed, accessible lesion with no local
invasion or metastases, although the recurrence rate can be as high as
50 - 68% in cases of large invasive tumors [25]. Local treatment
primarily involves surgical excision of the mass. Because most tumors
arise from the vestibule or the smooth muscle wall of the vagina, they
are usually removed per vulva [26,27].
Diagnosis of the tumors mass reported here is based on history,
clinical, histopathological and IHC findings. Biopsy for histological
examination is the most reliable method for diagnosis. If there is doubt
about the histological diagnosis, a definitive diagnosis can be made by
IHC studies. Histochemical and electron microscopic studies may be
needed for positive identification of the tumour [22].
In this study an episiotomy was performed in deep seated cases of
leiomyosarcoma, adenocarcinoma and vaginal TVT, at one o'clock
position relative to the dorsal commissure of the vulva to allow easier
visualization of the mass. It also assists in the surgical extirpation of
extraluminal tumors [1,4,26] . An unusual feature of one case of
vaginal leiomyosarcoma was the occurrence of partial vaginal prolapse
in bitch.
Since it has been found that vaginal tumors are hormone
dependent, ovariohysterectomy is the treatment of choice. This also
allows examination of the abdominal organs for the presence of
metastasis [1]. In this study, after owners' consultations,
ovariohysterctomy was performed in four bitches.
Vaginal leiomyoma is the most frequently occurring neoplasm
particularly in the bitch [1] while leiomyosarcomas are considered
uncommon lesions. Unlike our study in which all cases were
leiomysarcoma in where cells showing criteria of malignancy. Increase
incidence of leiomyosarcoma than leiomyoma may be attributed to
unstability in genes or environmental difference, so this needs further
studies. A vulvar and vaginal leiomyosarcoma are reported commonly
in goats [4] and cows[26]. Thatcher and Bradley (1983) reported that,
10 bitches out of 99 were detected as leiomyosarcoma and only one
occurred in a spayed female, in our cases all bitches were also intact
when the condition occurred.
Immunohistochemical positive staining for alpha-SMA was done to
confirm histopathological diagnosis that indicates all cases were
leiomyosarcoma. Where alpha-SMA considered a diagnostic feature of
genital leiomyosarcomas of domestic animals [28].
Perivlvar lipoma in bitch is properly detected grossly and
histopathologically where it arises from perivaginal adipose tissue and
form well circumscribed mass. Despite its exaggerated size in this
study, the completion of the surgical procedure was uneventful
without major difficulties or complications, which is common for this
type of neoplasm [29]. The dog had a complication to move due to the
size of the tumor, so much so that this was ulcerated due to friction
with the ground. The postoperative evaluation was totally favorable.
The bitch with perivulvar lipoma had neither difficulty in urinating
nor other physiological function, unlike other reports [29,30].
Benign vaginal polyps occur in older bitches [9]. These are usually
solitary, measure up to several centimeters in diameter and are
attached to the vaginal wall by a thin stalk. Our case is a type of vaginal
polyp; it was diagnosed as vaginal cystic polyp. There are no detailed
reports of their microscopical feature, where our microscopic
examination showed cystic dilation filled with serous fluid and
Volume 4 • Issue 2 • 1000158
immunohistochemistry was positive for desmin that differntiate it
from leiomyoma . A report of 13 cases of fibro epithelial vaginal polyp
in bitches was studied [9].
A metastatic mammary adenocarcinoma, with intralymphatic
metastases in the vestibule, has been described in dogs [6,14]. Our
cases did not have a history of mammary gland neoplasia and ancillary
diagnostics that preceded the surgical excision (abdominal
ultrasonography) did not document systemic involvement. At the time
of surgery, the mass was localized to the vaginal vestibule.
Histopathologically, anaplastic cells tend to form characteristic acini of
adenocarcinoma. Local recurrence and/or metastatic disease were not
noted on follow-up examination at about 2 years post-surgery.
Canine TVT are cauliflower-like, pedunculated, and nodular, may
be solitary or multiple and are almost located on the genitalia [17, 18,
31]. Histopathologically it was well diffentiated which complete close
to histiocytic origin and characteristic arrangements in sheets or cords
radially around blood vessels. They may be transplanted to adjacent
skin and oral, nasal, or conjunctival mucosae as seen in a case in the
current study. Sometimes the tumor may arise deep within the
prepuce or vagina. In the current study, the TVT mass is located on
the caudal part of the penis, from the crura to bulbis glandis and on
the prepuce as described by [22]. TVT is rarely metastasis and this
really indicated by intense lymphocytic infiltration in dermis of
prepuce and vaginal submucosa in this study. Surgical resection was
performed with a satisfactory results and no recurrence. Surgical
resection in combination with L-asparaginase and prednisone may be
an option for dogs with TVT that does not respond to standard
chemotherapy treatment with vincristine and/or doxorubicin as
mentioned by [32].
Conclusion
Surgical excision of canine genital tumors alone or combined with
an episiotomy was successful with no recurrence. TVT is rarely
metastasis and this really indicated by intense lymphocytic infiltration
in dermis of prepuce and vaginal submucosa of our cases. There are no
detailed reports of microscopical feature of vaginal cystic polyp in
canine.
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Citation:
Rizk AZ, Hamed MF, Zaghloul AE (2015) Retrospective Study of Some Tumors at the Genital Tract of Dogs. J Vet Sci Med Diagn 4:2.
doi:http://dx.doi.org/10.4172/2325-9590.1000158
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