Telos® High Throughput Droplet System

The Dolomite Centre Ltd
Dolomite Microfluidics – North America Office
Unit 1, Anglian Business Park, Orchard Road,
Royston, Hertfordshire, SG8 5TW, UK
Blacktrace Inc, 29 Albion Place
Charlestown, MA 02129, USA
T:
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+44 (0)1763 242491
+44 (0)1763 246125
[email protected]
www.dolomite-microfluidics.com
617 803 6655
617 848 1121
617 500 0136
[email protected]
Telos® High Throughput Droplet
System
product datasheet
Description
Benefits
System Specifications
Applications
Typical Telos® System setup
Fluidic Pathway
Orientation
Assembly
Telos® Modules
Telos® Accessories
Telos® Spares
Droplet formation
IP License
Droplet Monitoring Software
Customisation
The Dolomite Centre Limited
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Part name
Part number
Page
Telos® Clamp Module
3200400
9
Telos® Clamp Module - Tube Collection
3200399
10
Telos® Support Frame
3200375
11
Telos® Starter Kit
3200363
12
Telos® Purging Chip (Pack of 5)
3200370
13
Telos® Remote Chamber 3.8L
3200408
14
10µm PEEK B-o-B Filter, 1/16” (Pack of 5)
3200409
15
Telos® Valve Block
3200364
17
Telos® Standard Interface
3200365
18
Telos® Tube Collection Interface
3200366
19
Telos® Fittings Pack
3200367
20
Telos® Chip FFKM Seal
3200368
21
Telos® Plug Set
3200369
21
10µm PEEK Filter, FFKM (Pack of 10)
3200372
22
FFKM O-ring (Pack of 10)
3200373
22
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Description
The Telos® System is a breakthrough product for parallel microfluidic processes,
including emulsion generation, micro-particle production, high throughput mixing and
microreactions. This modular, scalable, and highly flexible system removes the low
throughput limitation of individual microfluidic chips, enabling litres of droplets or particles
to be produced in a day.
Up to 10 Telos® modules can be assembled in parallel. Each module holds a microfluidic
chip typically with 7 junctions, enabling a total of 70 parallel junctions to be run at once. All
junctions are visible from above and below for illumination and optical access. The
modules also have integrated valves providing excellent flow control during priming and
operation and optional integrated filters on all input streams. The Telos® system can be
configured to allow collection of the output streams into a bulk fluid reservoir or into
parallel tubing to allow downstream process steps.
Telos® operates from 0-10bar (150psi) and 4-80°C. Fast set-up time is achieved with
tool-free assembly of modules, chips, valve blocks and connectors.
A 10 module Telos® System made from Telos® Support Frame (Part No. 3200375) and 10 x Telos® Clamp Module
– Tube Collection (Part No. 3200399)
Benefits
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Enables high throughput microfluidics
Tool - free system set up
Wide temperature and pressure range
Excellent chemical compatibility
Optical access above and below chip
Excellent flow control during priming and operation
Scalable with up to 10 chips in parallel
Various configurations possible to suit many applications
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System Specifications
Specification
Footprint
Depth: 100mm, Width: 60 – 300mm, Height:
105mm
Number of Ports
3
Output configuration options
Surface Connector, Edge Connector, bulk fluid
reservoir
Number of chips in Parallel
1 – 10
Chip compatibility – Linear Connector*
Width: 15mm, Thickness: 4mm, Length: 45mm
Chip compatibility – Edge/Manifold*
Width: 15mm, Thickness: 4mm, Length: 22+mm
Operating pressure
0 – 10bar
Operating temperature
4 – 80°C
Chip materials
Glass, Quartz, other options available on request
Wetted materials
FFKM, PEEK, PCTFE, PTFE
Internal volume per Telos® Clamp Module
Emulsion Flow rate per Telos® Clamp
Module
207µl
~ 10ml/min**
*Note: Surface connection required, for hole positions see Telos® 2 Reagent Chip
**Example fluids: water in decane
Applications
Telos® benefits a wide range of industries including pharmaceutical, food, agrochemical,
cosmetics and research and can be used for the following applications:

High throughput emulsion and foam generation

Microparticle and nanoparticle synthesis

Novel product formulation

DNA, cell and bead encapsulation

Parallel Micromixing and micro-reactions
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Typical Telos® System setup
The diagram below shows a typical Telos® system setup with Dolomite P-Pumps, remote
chambers and flow sensors controlling the flow of three input streams:
For more information on system setup please refer to the Telos® application notes.
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Fluid Pathway
Below is a schematic that shows the fluid pathway for the Telos® system. Input tubes
connected via the Telos® Support Frame to supply input fluids to all of the Telos® Clamp
Modules. Each Telos® Clamp Module has three on/off valves which control the flow of the
input fluid streams to each chip. Each Telos® Clamp Module also has three in-line filters
which are replaceable and are situated just before the flow reaches the chip.
Key:
- Valve
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- Filters
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Vertical Orientation
In this orientation a fluid reservoir can be placed under the chips and filled so that the
ends of the chips are submerged. This allows the droplets to be collected in a bulk
solution.
In the image below four legs hold the Telos® in the vertical orientation. The legs are
normally retracted into the Telos® support frame and are extended using the release
buttons.
Horizontal Orientation
In horizontal orientation the output streams are typically collected into tubing using the
Telos® Clamp Module - Tube Collection (Part No. 3200399). For system with two input
streams it is possible to use the fluid input channel on the Telos® to collect the output
stream from the chip.
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Assembly
Multiple Telos® clamp modules can be stacked together to build up a system as shown
below
Telos® Clamp
Module
Telos® Support Frame (end
caps)
After the system is assembled chips can be inserted and clamped down using a force
limited lever mechanism which avoids overcompression of the chips and seals. Input
tubing is connected using the nut and ferrule connection which is supplied with the Telos®
Support Frame.
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Telos® Clamp Module (Part No. 3200400)
The Telos® Clamp Module holds a microfluidic chip with up to 7 channels. Each junction
is visible from above and below for illumination and optical access. Fast set-up time is
achieved with an easy to use tool-free clamp mechanism that locates and seals the chip
in place, making or breaking connections instantly.
The Telos® Clamp Module incorporates the 3 in-line on/off valves for excellent flow
control during priming and operation. Additionally, the Telos® Clamp Module includes
optional integrated filters.
Multiple Telos® Clamp Modules can be stacked side by side to scale up production
allowing on/off control of fluid throughput streams for each chip. Up to 10 of these
modules can be added, giving a total of 70 parallel channels. The Telos® Clamp Module
is configured to allow vertical product collection into bulk fluid. Telos® operates from 010bar (150psi) and 4-80°C.
The Telos® Clamp Module works in conjunction with the Telos® Support Frame (Part No.
3200375).
Telos® Clamp Module (Part No. 3200400)
Specification
Number of ports
3
Internal volume
207µl
Operating pressure
10bar*
Operating temperature of connector
Wetted materials
4 to 80ºC
PCTFE (body), PTFE (rotor), PEEK (seal block), FFKM (seals)
*Tested at 21ºC
Included Parts
Telos® Standard Interface
1
Telos® Valve Block
1
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Telos® Clamp Module – Tube Collection (Part No. 3200399)
The Telos® Clamp Module - Tube Collection holds a microfluidic chip with up to 7
channels. Each junction is visible from above and below for illumination and optical
access. Fast set-up time is achieved with an easy to use tool-free clamp mechanism that
locates and seals the chip in place, making or breaking connections instantly.
The Telos® Clamp Module – Tube Collection incorporates the 3 in-line on/off valves for
excellent flow control during priming and operation. Additionally, the Telos® Clamp
Module includes optional integrated filters.
Multiple Telos® Clamp Modules can be stacked side by side to scale up production
allowing on/off control of fluid throughput streams for each chip. Up to 10 of these
modules can be added, giving a total of 70 parallel channels. The Telos® Clamp Module Tube Collection is configured to allow droplets to be collected directly into FEP or similar
tubing. Telos® operates from 0- 10bar (150psi) and 4- 50°C.
The Telos® Clamp Module - Tube Collection works in conjunction with the Telos®
Support Frame (Part No. 3200375).
Telos® Clamp Module – Tube Collection (Part No. 3200399)
Specification
Number of ports
3
Internal volume
207µl
Operating pressure
10bar*
Operating temperature of connector
Wetted materials
4 to 50ºC
PCTFE (body), PTFE (rotor), PEEK (seal block), FFKM (seals)
*Tested at 21ºC
Included Parts
Telos® Tube Collection Interface
1
Telos® Valve Block
1
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Telos® Support Frame (Part No. 3200375)
The Telos® Support Frame slots onto the ends of a series of Telos® Clamp Modules
(Part Nos. 3200399 and 3200400) creating a system with up to 10 Telos® Clamp
Modules.
One end of the Telos® Support Frame has fluid input ports which are fed by Mitos PPumps (Part No. 3200016).
The Telos® Support Frame has adjustable legs to set the height for vertical orientation of
the chip output over a liquid collection reservoir (see Telos® Clamp Module Part No.
3200400).
Telos® operates from 0-10bar (150psi) and 4-50°C. Fast set-up time is achieved with
tool-free module assembly.
Telos® Support Frame (Part No. 3200375)
Specification
Number of ports
Operating pressure
Operating temperature of connector
Wetted materials
3
10bar*
4 to 50ºC
FFKM (seals), FEP (tubing)
*Tested at 21ºC
Included Parts
FFKM O-ring
1
Flangeless Ferrule, ETFE
3
FFKM O-Ring
3
Short Headless Nut
2
Long Headless Nut
1
PTFE Tube Cutter
1
FEP Tubing, 1/16" x 0.8mm, 10 metres
1
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Telos® Starter Kit (Part No. 3200363)
The Telos® Starter Kit allows evaluation of the Telos® chip range, prior to the purchase of
a full Telos® system. This starter kit is perfect for initial experimentation, for example
testing a single Telos® 2 Reagent Chip (Part No. 3200357), before scaling up to multiple
chips.
Telos® Starter Kit (Part No. 3200363)
Specification
Inputs
3 surface
Output
1 edge
Operating pressure
Operating temperature of connector
Operating temperature of fluid in connector
Chip materials
Wetted materials
0 – 10bar
-15 – 50°C *
-15 – 150°C **
Glass, Quartz, other options available on request
FEP or PEEK (tubing), FKM (seal), FFKM (seal), PEEK,
ECTFE, Glass (chip)
Chip thickness options
4mm (+ 0.2 mm / - 0.05mm)
Chip width options
15mm (+ 0.02mm / - 0.2mm)
Chip length options
45mm (+ 0.2 mm / -0.05mm)
*Upper temperature limit applies to PEEK tubing. FEP tubing is limited to 40ºC
**Assumes external surfaces of connector are at room temperature and tube I.D. <0.3mm.
Included Parts
Linear Connector 7-way
1
Linear Connector Funnel
3
Triple Top H Interface (45mm)
1
PTFE Tube Cutter
1
FEP Tubing, 1/16" x 0.8mm, 10 metres
1
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FEP Tubing, 0.8 x 0.1mm, 10 metres
1
Telos® Purging Chip (Pack of 5) (Part No. 3200370)
The Telos® Purging Chip is an accessory for the Telos® Clamp Modules (Part Nos.
3200399 and 3200400).The purging chip can be loaded into the position of a droplet chip
and allows the Telos® feed lines to be purged, primed or cleaned. This low cost chip is
unlikely to become blocked as it has very large channels. Used before starting an
experiment this chip will prolong the life of the Telos® chips.
The primed fluids drip off the end of the chip and can be collected in a suitable container.
Telos® Purging Chip (Pack of 5) (Part No. 3200370)
Benefits
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Enables the Telos® system to be rapidly purged before use
Allows chips to be disconnected during Telos® cleaning
Excellent chemical compatibility (PTFE)
Reduces set up time when using viscous fluids
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Telos® Remote Chamber 3.8L (Part No. 3200408)
The Telos® Remote Chamber 3.8L is a 10bar stainless steel pressure chamber
accessory for use with the Telos® system. Using a Mitos P-Pump (Part No. 3200016) it
enables large sample volumes up to 3.8L to be used, so that continuous pumping for long
periods is possible. With the precise pressure-driven pumping mechanism of the Mitos PPump, the flow from the chamber is highly stable and pulse-free. The Telos® Remote
Chamber 3.8L is simple to connect and comes pre-fitted with a 10µm PEEK B-o-B Filter
(Part No. 3200409).
Telos® Remote Chamber 3.8L (Part No. 3200408)
Benefits
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Sample volumes up to 3.8 litres
In-tank filter supplied
Continuous pumping for extended periods of time
Simple to connect to the Telos® system
Suitable for use with 1/8” or 1/16” tubing
Can be pressurised up to 10bar*
Excellent chemical compatibility
Robust stable design fitted with stainless steel base
Marine grade stainless steel
Temperature range 4 - 80ºC
*note tested at 21˚C
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10µm PEEK B-o-B Filter, 1/16” (Pack of 5) (Part No. 3200409)
The 10µm PEEK B-o-B Filter, 1/16” (Pack of 5) is designed to fit onto the end of 1/16”
tubing enabling inlet fluids to be filtered at the bottom of the bottle or reservoir.
The large surface area allows operation at high flow rates and is recommended for use in
Telos® Remote Chamber 3.8L (Part No. 3200408) and Mitos P-Pump Remote Chamber
400 (Part No. 3200043).
These filters are required to prevent debris/particulates entering microfluidic chips.
The filter has an extra port providing the option to bubble an inert gas, such as Helium,
through the filter to de-gas the sample.
10µm PEEK B-o-B Filter, 1/16” (Pack of 5) (Part No. 3200409)
Benefits
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Bottom of Bottle design enabling full sample usage
Large surface area
Excellent chemical compatibility (PEEK)
Prolongs the life of microfluidic chips
De-gassing port for optional use
10µm filter
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Telos® spares
Telos® Valve Block
3200364
Telos® Standard Interface
3200365
Telos® Tube Collection Interface
3200366
Telos® Fittings Pack
3200367
Telos® Chip FFKM Seal
3200368
Telos® Plug Set
3200369
10µm PEEK Filter, FFKM (Pack of 10)
3200372
FFKM O-ring (Pack of 10)
3200373
Telos® Spares Exploded View
10µm PEEK Filter,
FFKM (Pack of 10)
(Part No. 3200372)
Telos® Chip FFKM Seal
(Part No. 3200368)
Telos® Fittings Pack
(Part No. 3200367)
FFKM O-ring
(Part No. 3200373)
Telos® Plug Set
(Part No. 3200369)
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Telos® Valve Block
(Part No. 3200364)
Telos® Standard Interface
(Part No. 3200365)
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Telos® Valve Block (Part No. 3200364)
The Telos® Valve Block is a spare for the Telos® Clamp Modules (Part Nos. 3200399
and 3200400). Each Telos® Valve Block includes 3 on-off valves which allow excellent
flow control during priming and operation.
Note that the seals provided as standard in this valve are made from perfluoro-elastomer
(FFKM), which is not compatible with fluorinated oils. To have Telos® Valve Block
supplied with seals that are compatible with fluorinated oils please contact Dolomite.
Telos® Valve Block (Part No. 3200364)
Benefits
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Pre-chip replaceable filters
Fast valve response
High pressure capability
Excellent chemical compatibility
Robust design
Small space envelope
Specification
Number of ports
3
Internal volume
178µl
Operating pressure
10bar*
Operating temperature
Wetted materials
4 to 80ºC
PCTFE (body), PTFE (rotor)
10µm PEEK Filter, FFKM
3
FFKM O-ring
3
*Tested at 21ºC
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Telos® Standard Interface (Part No. 3200365)
The Telos® Standard Interface is an accessory for the Telos® Clamp Module (Part No
3200400). The Telos® Standard Interface is also interchangeable with the Telos® Tube
Collection Interface (Part No 3200366) allowing for different output collection
configurations.
Note that the seals in this interface are made from perfluoro-elastomer (FFKM), which is
not compatible with fluorinated oils. To have Telos® Standard Interface supplied with
seals that are compatible with fluorinated oils please contact Dolomite.
Telos® Standard Interface (Part No. 3200365)
Specification
Number of ports
3
Internal volume
29µl
Chip thickness options
4mm (+ 0.2 mm / - 0.05mm)
Chip width options
15mm (+ 0.02mm / - 0.2mm)
Chip length options
22 – 90mm
Operating pressure
10bar*
Operating temperature
Wetted materials
Telos® Chip FFKM Seal
4 to 80ºC
PCTFE (body), PTFE (rotor), PEEK (seal block), FFKM (seals)
1
*Tested at 21ºC
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Telos® Tube Collection Interface (Part No. 3200366)
The Telos® Tube Collection Interface is an accessory for the Telos® Clamp Module –
Tube Collection (Part No. 3200399). The Telos® Tube Collection Interface is also
interchangeable with the Telos® Standard Interface (Part No. 3200365) allowing different
output configurations.
Note that the seals in this interface are made from perfluoro-elastomer (FFKM), which is
not compatible with fluorinated oils. To have Telos® Tube Collection Interface supplied
with seals that are compatible with fluorinated oils please contact Dolomite.
Telos® Tube Collection Interface (Part No. 3200366)
Specification
Number of ports
3
Internal volume
29µl
Chip thickness options
4mm (+ 0.2 mm / - 0.05mm)
Chip width options
15mm (+ 0.02mm / - 0.2mm)
Chip length options
45mm (+ 0.2 mm / -0.05mm)
Operating pressure
10bar*
Operating temperature
Wetted materials
Telos® Chip FFKM Seal included
4 to 50ºC
PCTFE (body), PTFE (rotor), PEEK (seal block), FFKM/FKM (seals)
1
*Tested at 21ºC
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Telos® Fittings Pack (Part No. 3200367)
This pack of fittings and tubes are required to connect the Mitos P-Pumps to the Telos®
system. Included in this pack is a tube cutter to enable successful tube termination. All
parts have excellent chemical resistance.
Telos® Fittings Pack (Part No. 3200367)
Contents
Quantity
Flangless Ferrule, ETFE
15
Short Headless Nut
10
Long Headless Nut
5
PTFE Tube Cutter
1
FEP Tubing, 1/16" x 0.8mm, 10 metres
2
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Telos® Plug Set (Part No. 3200369)
This is a spare for the Telos® Support Frame (Part No. 3200375). The Telos® Plug Set’s
functionality is to eliminate trapped air and seal the fluid input channels at one end of a
series of Telos® Clamp Modules (Part Nos. 3200399 and 3200400).
The Telos® Plug Set contains 3 sets of 3 plugs.
Telos® Plug Set (Part No. 3200369)
Telos® Chip FFKM Seal (Part No. 3200368)
The Telos® Chip FFKM Seal is an accessory for the Telos® Clamp Modules (Part Nos.
3200399 and 3200400). The Telos® FFKM Seal provides excellent chemical resistance
and reliable sealing to Telos® chips.
Note that this seal is made from perfluoro-elastomer (FFKM), which is not compatible with
fluorinated oils. For seals that are compatible with fluorinated oils please contact
Dolomite.
Telos® Chip FFKM Seal (Part No. 3200368)
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10µm PEEK Filter, FFKM (Pack of 10) (Part No. 3200372)
The 10µm PEEK Filter, FFKM is an accessory for the Telos® Clamp Modules (Part Nos.
3200399 and 3200400). Each Telos® Clamp Module contains three of these filters
allowing filtration for up to 3 input streams. These filters are required to prevent debris /
particulates entering Telos® chips.
Note FFKM is not compatible with Fluorinated oils. For seals that are compatible with
fluorinated oils please contact Dolomite.
10µm PEEK Filter, FFKM (Pack of 10) (Part No. 3200372)
FFKM O-ring (Pack of 10) (Part No. 3200373)
The FFKM O-Ring is a spare for the Telos® Clamp Modules and Support Frame (Part
Nos. 3200375, 3200399 and 3200400). These FFKM O-Rings make a liquid seal between
modules on the input liquid flow paths.
Note that these seals are made from perfluoro-elastomer (FFKM), which is not compatible
with fluorinated oils. For seals that are compatible with fluorinated oils please contact
Dolomite.
FFKM O-ring (Pack of 10) (Part No. 3200373)
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Droplet Formation
The size, consistency, and production rate of droplet formation is a function of several
physical parameters, including:

Channel size

Viscosity and surface tension of the various fluids

Presence of surfactants

Miscibility of the fluids

Use of hydrophobic or hydrophilic coating on the channel walls

Total flow rate and relative flow rate of each fluid

Flow stability
To accelerate development work in droplet microfluidics, Dolomite offers a range of
modular micro droplet systems featuring Dolomite’s industry leading microfluidic pumps,
connectors and chips. This features the Mitos P-Pump, which provides stable, pulse-free
flow for generation of droplets with extremely consistent diameters (monodisperse).
Please contact Dolomite to configure a droplet system that fits your requirements.
Mitos P-Pump, Flow Resistors and Droplet Chips featured in the Droplet Advanced System
IP license
Dolomite is a licensee of Japan Science and Technology Agency (“JST”) under JST’s
microdroplet generation technology. Please see our website for further details.
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Droplet monitoring software
The Droplet monitoring software has been developed to take a media file generated from
a high speed camera and analyse the size range of droplets produced.
Customisation
Custom chips can be designed for this system to take full advantage of scale up potential.
If you would like to generate droplets of a different size, operate with challenging fluids or
perform complex droplet functions, Dolomite can design the junction geometry required.
The system lends itself well for customisation due to the modular nature of the design.
Some reasons for customisation:
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Real time product Quality Control
Temperature and atmospheric control
Production of core/shell particles
Disposable fluidic pathways
Chemical compatibility
Automation
More chips in parallel
The range of Dolomite services available covers all aspects of the development process
from characterization of liquids for droplet generation to the design of commercial
instruments in the field of droplet microfluidics. Please contact Dolomite to discuss your
application.
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