Experimental Animal Division

Transcription

Head
Atsushi YOSHIKI, Ph.D.
Goal
The Experimental Animal Division has two missions: (a) to
Laboratory, European Mouse Mutant Archive (EMMA)
collect, preserve, conduct quality control of and distribute
and other centers, and has participated in the International
high-quality mouse resources, and (b) to develop novel mouse
Mouse Strain Resource (IMSR) to distribute mouse strains
models and useful technologies for bioresource activities. Our
worldwide. To meet research and social needs, our mice are
ultimate goal is to contribute to human welfare by facilitating
cleaned up to a specific pathogen-free state, strictly monitored
research in life sciences with our mouse resources. The mouse
of their health, and accurately tested on their genetic
is one of the most superior model organisms used in life
modifications and backgrounds. Phenotypic information of
sciences to understand human health and overcome diseases.
the strains is frequently updated to enrich their value. With
Since 2002, RIKEN BRC has been designated as the central
these activities, RIKEN BRC plans to build mouse resources
core facility for mouse resources in Japan by the National
that meet the highest global standards by 2010. The Material
BioResource Project (NBRP) of MEXT. With support from
Transfer Agreement (MTA) is used to protect the intellectual
the scientific community, RIKEN BRC has collected over
property rights of the developer of the strains. Training
3,200 mouse strains including genetically modified models,
courses concerning advanced technologies are provided for
ENU mutants, and inbred and wild-derived strains. RIKEN
users to best use our resources. RIKEN BRC collaborates
BRC is a founding member of the Federation of International
with Asian and other overseas institutes to contribute to the
Mouse Resources (FIMRe) together with The Jackson
advancement of life sciences in the world.
Programs
I. Bioresource program: Collection, preservation, qualitycontrol and distribution of the mouse resources
II. Development program: Development of novel mouse
models and technologies necessary for program I.
Members
Senior Scientist, Head of Experimental Animal Division
Atsushi YOSHIKI, Ph.D. (2004.12~)
Senior Research Scientist
Fumio IKE, Ph.D. (2001.4~)
Senior Technical Scientist
Noriko HIRAIWA (2001.4~)
Hatsumi NAKATA, Ph.D. (2007.4~)
Kazuyuki MEKADA, Ph.D. (2003.8~)
Yasuyuki KITAURA, Ph.D. (2006.3~）
Ayumi MURAKAMI (2001.4~)
Maiko IJUIN (2004.10~)
Masayo KADOTA (2006.4~) Mika OKAWA (2006.4~2008.1)
Research Scientist
Technical Staff Ⅱ
Assistant
Norie TSUDA (2001.12~2007.3)
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RIKEN BRC Annual Report 2005 ～ 2007
Student Trainee
Jan, MEI-LING (2007.4~)
Visiting Scientist
Chikako YOSHIDA-NORO, Ph.D. (2005.4~)
Ikuko SAKAI (2005.8~)
Chino SASAOKA (2005.8~)
Ryoji HYODO (2006.11~)
Nobuhiko ONDA (2005.8~2007.3)
Kaori HIGUCHI (2006.11~2008.3)
Kayuri MURAKI (2006.11~2008.3)
Visiting Technician
Ayako KAJITA (2006.5~)
Agency Staff
Fujimi ARAI (2001.4~)
Megumi KOBAYASHI (2001.4~)
Reiko KOJIMA (2001.4~)
Akemi KOSHIYAMA (2001.4~)
Kyoko MEKADA (2001.4~)
Yukiko SEKI (2001.4~)
Aya UEKI (2001.4~)
Masaru ONUMA (2001.5~)
Atsushi CHOEI (2001.6~)
Rika TAKASHIMA (2001.6~)
Teruo SAITO (2004.12~)
Miki NAKAYAMA (2005.5~)
Chihiro YOKOYAMA (2005.10~)
Kinuko ITO (2005.10~)
Yusuke OBA (2005.12~)
Reiko KAWAI (2006.1~)
Hitomi TAKAHASHI (2006.4~)
Masashi YOKOTA (2006.4~)
Chiharu OKUBO (2006.5~)
Akemi YASUI (2006.5~)
Masashi OZAWA (2006.6~)
Masako KITO (2006.9~)
Hiroyuki OKAMOTO (2006.9~)
Tomomi HASHIMOTO (2007.4~)
Makiko IIZUMI (2007.4~)
Naoki OTAKA (2007.4~)
Chieko YAMADA (2007.4~)
Tomoe SAKAI (2007.4~)
Maki YAMAMOTO (2007.6~) Miki NAKAYAMA (2007.11~)
Chie FUJIMOTO (2008.4~)
Aya MIMORI (2008.4~)
Kyuichi TAGUMA (2008.4~)
Tomoko TAKAHASHI (2008.4~)
Naoki HIRANO (2008.6~)
Hiromi HISAMATSU (2007.6~)
Fumie MIYAZAKI (2001.4~2008.3)
Masami ICHIKAWA (2007.3~2008.3)
Hitomi OKANO (2007.3~2008.3)
Sanae MIYAMOTO (2006.11~2007.3)
Masayuki SAITO (2006.10~2007.11)
Satoe NAKAMURA (2005.4~2008.3)
Mika KOMURO (2005.6~2007.2)
Yoko ONUKI (2005.10~2007.10)
Chie HOSHI (2005.11~2007.11)
Naomi ISO (2006.2~2007.3) Junichi MASUDA (2006.3~2008.7)
Emiko FUKUDA (2006.4~2008.3)
Yasuko NISHIKAWA (2006.4~2007.9)
Ayako KAJITA (2006.5~2007.3)
Katsumi WATANABE (2007.11~2008.5)
Yuko FUJIMOTO (2001.4~2007.3)
Noriko KATAOKA (2001.4~)
Tomohiro OKUBO (2002.2~)
Keiko TOMIYAMA (2002.3~)
Noriko NOGUCHI (2004.1~)
Kunihiro SAKURAI (2005.2~)
Mariko HASEGAWA (2006.3~)
Takeshi MATSUZAKI (2006.3~)
Katsuhiko YAGISHITA (2006.7~)
Takashi FURUYA (2007.2~)
Takayoshi KOSHIDO (2007.4~)
Takeshi NAGAO (2007.4~)
Masaki YAMAGUCHI (2007.4~)
Kiyohiko ASANO (2007.8~)
Masataka ASANO (2007.12~)
Contract Staff
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Takehiko FUJISAWA (2008.4~) Haruo SUZUKI (2004.4~2007.3)
Sei CHOEI (2001.4~2007.2)
Katsuya ONUKI (2001.4~2008.2)
Chieko YAMADA (2001.4~2008.3)
Hiroyuki NUMAJIRI (2001.8~2007.5)
Naoki OTAKA (2001.9~2008.3)
Masaki MIYAKE (2006.8~2007.3) Asami SAGARA (2006.2~2007.12)
Yasuhiro YOKOTA (2002.10~2007.1)
Minehisa SUZUKI (2007. 4~2007. 8)
Okamoto, Hirano, Koshido, Ike, Yoshiki, Asano(T), Matsuzaki, Kitaura
Asano(K), Sakurai, Yamaguchi, Saito, Okubo(T), Yokota, Masuda, Taguma, Furuya
Mitsunari, Hasegawa, Kojima, Arai, Oba, Nagao, Onuma, Otaka, Choei, Takahashi(H)
Yamamoto, Hashimoto, Iizumi, Kawai, Nakayama, Hisamatsu, Kajita, Okubo(C), Nakayama, Fujisawa
Kobayashi, Sato, Mimori, Sakai, Takahashi(T), Ito, Koshiyama, Ozawa, Takano
Okada, Murakami, Shima, Fujibayashi, Yokoyama, Hiraiwa, Kataoka, Kito, Tsukahara
Fujimoto, Tomiyama, Takashima, Yamada, Yasui, Ijuin, Kadota, Ueki
Programs
Council.
1. Bioresource Program
MTAs for the deposition and distribution of
mouse resources have been used to protect the intellectual
(1) Collection, preservation and distribution of mouse
strains
property right of the Developer, and to clarify the terms and
conditions of use for the Recipient of the biological resources,
This program has been operated in cooperation with research
respectively. We have collected over 3,200 strains mainly
scientists, technical scientists, technical staffs, agency and
developed in Japan, such as inbred, transgenic, knockout, Cre-
contract staffs. In regard to the annual plan for the operation
driver, ENU mutant and wild-derived strains, as well as gene-
of the Experimental Animal Division, we have obtained
trap ES clones. These strains are useful models for the study
useful advice and suggestions from distinguished members
of cancer, immunity and allergy, endocrine diseases, brain
of the BRC Experimental Animal Steering Committee, BRC
and neurological disorders, development and differentiation
Advisory Council, Promotion Advisor and RIKEN Advisory
abnormalities, and sensory organ abnormalities (Fig.1).
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colonies. The distribution of frozen strains is conducted after
assessing their recovery. By July 2008, 2,123 strains have
been preserved as frozen embryos or sperm.
(4) Quality control
(i) Microbiological monitoring
After cleanup treatments, the mice are monitored by a 2nd
round of microbiological tests for 19 pathogens. If the mice
are free of specific pathogens, they are transferred to the
BRC breeding rooms in the barrier. Periodic microbiological
monitoring of 19 or 22 major pathogens has been done in
Figure 1. Framework of collection and distribution of
mouse resources for advancement of life sciences.
every rack of the facility using sentinel mice (70,770 tests
for 3,681 mice from October 2005 to July 2008). The
Mouse strains with high-demand from the community are
environment of the facility is quarterly monitored for bacteria
maintained as live animals, and others with low-demand
and fungi at 512 points.
are preserved as frozen embryos. With the release of new
(ii) Genetic monitoring
mouse resources and changes in circumstances, we revised
Genetically modified strains such as transgenic and knockout
the distribution fees in March, 2008. The number of
mice are genotyped using PCR protocols with allele-
registered users is 2,459 (523 for overseas) as of July, 2008.
specific primers. The genotyping PCR protocols for over
We have distributed 8,453 (1,475 for overseas) mice to 436
400 strains with high-demand is available on our website as
(222 overseas) organizations since 2002. Recipients of our
downloadable pdf files. Genetic backgrounds of congenic
mice included both academic (88%) and for-profit (12%)
strains are monitored with 7 sets of simple sequence length
organizations.
polymorphism (SSLP) markers at 75-88 loci in each set.
(2) Cleanup of mouse strains
Inbred and wild-derived strains are also monitored with 15
The cleanup of mouse strains deposited to RIKEN BRC has
standard biochemical genetic markers.
contributed to the high quality standard of animal experiments
(iii) Immunological profiling
in Japan. All the strains are serologically tested for infection
The immunological profiling of 43 Inbred and wild-derived
by eight dangerous pathogens. Based on the result of
strains is conducted using flow cytometry by the fluorescent
serological tests, mice are transferred to the Bio-bubble
labeling of spleen cell differentiation antigens.
housing facilities either in negative or positive pressure. Mice
(5) Collection of relevant information and advertisement
are bred to establish colonies for rederivation by in vitro
In collaboration with the BRC Information Division, our
fertilization and embryo transfer or Cesarean section. Jcl:ICR
website is regularly updated and enriched of its content.
and BALB/cA-nu/+ females are used as recipients of embryo
Publications by users and relevant literature about the
transfer and as foster nursing mothers, respectively. BALB/
strains are frequently surveyed by direct email letters and
cA-nu/+ mothers have excellent nursing capability to accept
public databases. A revised list of our strains is submitted
various strains in BRC.
monthly to the IMSR and distributed to the international
(3) Cryopreservation of mouse embryos and sperm
scientific community. Periodic e-mail newsletters have been
The cryopreservation of embryos and sperm is a key
distributed to over 2,000 users. The e-mail newsletters contain
technology for the successful operation of the mouse
an article entitled “Mouse of the Month” (Fig.2), topic news
resource center. Two-cell stage embryos are stored frozen
and a report of our recent activities. The mouse resources
in a vitrification solution containing ethylene glycol, Ficoll
and activities of the division are advertised in symposia and
and sucrose (EFS). The sperm from mutant and genetically
academic meetings.
modified strains is also cryopreserved using raffinose and
(6) Training course
skim milk as cryoprotective agents. The recovery rate of
The Experimental Animal Division provides training
each frozen strain is carefully assessed prior to closing live
courses for animal facility managers and laboratory animal
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technicians. Each course includes theory and practice of the
(8) Others
mouse facility management and quality control programs such
Our Division has been designated as a NBRP-rat sub-center
as microbiological and genetic monitoring tests. We have thus
in collaboration with Prof. Serikawa, Kyoto University. Our
far accepted 12 trainees from local pharmaceutical and other
mission is to establish a backup storage of NBRP rat strains as
companies, the National Laboratory Animal Center of Taiwan,
frozen embryos and sperm.
and the Lanzoh Institute of Biological Products of China.
(7) International collaboration
2. Development Program
RIKEN BRC is a founding member of the Federation
Our division develops novel mouse resources and relevant
of International Mouse Resources (FIMRe). FIMRe is
technologies necessary for the collection, preservation,
a collaborating group of mouse repository and resource
quality-control and distribution of the mouse resources. The
centers worldwide, including The Jackson Laboratory and
following development programs were carried out in the fiscal
the European Mouse Mutant Archive (EMMA). We have
years 2006-2008.
participated in IMSR, a one-stop shop of mouse strains
(1) Development of novel mouse resources
available worldwide. RIKEN BRC promotes collaboration
(i) RIKEN BRC grant for R&D of genetically modified
mouse strains
with Asian countries. We are a founding member of the Asian
Mouse Mutagenesis and Resource Association (AMMRA).
RIKEN BRC publicly called for applicants in the fiscal years
On November 26, 2007, the Bio-Evaluation Center of Korea
2007 and 2008 for research and development programs
Research Institute of Bioscience and Biotechnology and
to generate novel genetically modified mouse strains
RlKEN BRC entered into a Memorandum of Understanding to
of immediate demand by the research community. The
promote cooperation in areas of mutual interest in laboratory
applicants were asked to propose a design of gene constructs
animal sciences. We started mutual visits with the Laboratory
for genetic modifications, and to produce and submit the
Animal Center, Institute of Cytology and Genetics, Siberia
recombinant gene constructs to BRC. BRC separately orders
Branch of Russian Academy of Sciences in 2008.
the third parties to make mouse strains after an open bid. The
specialist committee members including those of the BRC
Experimental Animal Steering Committee have selected 12
academic organizations to generate 103 gene constructs for
genetic modifications in 2007, and 11 organizations for 291
genes in 2008.
(ii) Development of genetically uniform mouse strains
Transgenic and knockout mouse strains with high-demand
such as GFP and human disease models were backcrossed
with standard inbred strains to produce genetically uniform
congenic strains.
(iii) Development of Cre-Zoo
Conditional knockout mouse resources have been generated
worldwide for the functional analysis of genes. Tissuespecific Cre-transgenic mouse strains are essential tools
for dissecting spatiotemporal gene functions together with
conditional knockout mice. In cooperation with the Gene
Engineering Division, we developed several lines of Cretransgenic mouse strains driven by tissue-specific promoters.
Figure 2. An excellent mouse model is introduced
as “Mouse of the Month” in our e-mail mews.
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(iv) RIKEN BRC Mouse Phenome Database (RMPD)
RMPD was developed in collaboration with the BRC
Information Division and made available in our website in
March, 2008. The database includes external measures, blood
pressures, blood cell counts, and blood biochemistry measures
of 140 mouse strains. The data could be displayed as bar
graphs, two-dimensional plots and lists. We also collected
images of the coat colors, histopathological sections and
X-ray images of the skeleton of 104 various inbred and mutant
mouse strains. Behavioral phenotypes were also recorded
using a digital video camera.
(2) Development of technologies for quality control and
(v) Development of novel phenotype data and disease
models
strain characteristic database
(i) Development of a fine detection method for pathogens
We started collaborations with the Technology and
A highly sensitive multiplexed microfluidic immunoassay
Development Team for Mouse Phenotype Analysis, Japan
system for rapidly detecting pathogenic microbes with a
Mouse Clinic and other groups to enrich the phenotype data of
microvolume of blood sample was developed. Pathogenic
our mice and develop novel human disease models.
antigens were extracted and purified for the detection system
(3) Other projects
and positive controls. Genomic materials of 21 pathogenic
(i) NBRP genome information upgrading program
microbes were also been prepared as positive controls for
The end sequencing of 800,000 cDNA clones from wild-
microbiological tests.
derived strains, MSM, HMI, PGN2 and KJR was carried
(ii) Microsatellite and SNP data
out by collaborating with Prof. Shiroishi of the National
We prepared 7 sets of SSLP markers at 75-88 loci in each set
Institute of Genetics and Dr. Abe of the BRC Technology and
to examine genetic backgrounds. SNP analysis was carried
Development Team for Mammalian Cellular Dynamics.
out in 153 strains including inbred, congenic, recombinant
(ii) NBRP fundamental technology upgrading program
(2007-2008)
inbred and wild-derived strains to clarify their genetic quality
using 15K SNPs of B6-MSM and the Illumina Golden Gate
A collaborative program with Prof. Serikawa (Kyoto
Mouse SNP panel. These SNP data clearly demonstrated
University), Prof. Nakagata (Kumamoto University) and
the genetic relationship of the strains and the subtle genetic
Dr. Ogura (Bioresource Engineering Division) entitled
differences among C57BL/6 substrains.
“Development of transportation system for the mouse and
(iii) Development of genotyping methods for genetically
rat resources” was carried out to develop high-performance
modified strains
shipping containers for live rodents and their embryos and
We d e v e l o p e d a “ K O s u r v e y ” P C R p r o t o c o l f o r
sperm.
simultaneously detecting different regions of complex
(iii) Development of Cre-driver strains for neural circuit
genetics
transgenes, and used it to screen mice in the quarantine and
barrier facilities. Transgenic strains were analyzed with their
In collaboration with Prof. Tonegawa (MIT), Dr. Itohara
genomic sequences flanking transgenes. The information
(RIKEN BSI), Dr. Abe (BRC) and Dr. Obata (BRC), we
obtained was used to determine the integration site of
started a project to generate Cre-driver transgenic mouse
transgenes and to set up genotyping PCR protocols for hemi-
strains for the functional analysis of the neural circuit.
or homo-zygous distinction. Allele-specific PCR protocols
for accurately detecting point mutations and SNPs of the
strains were developed. These technologies and information
have contributed to obtaining accurate information on genetic
modifications and genetic backgrounds.
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Publications
【Original Papers】(*Peer reviewed journals)
1. Ugai H., Murata T., Nagamura Y., Ugawa Y., Suzuki
A., Sakurai N., Yoshiki A., Kusakabe M., Moriyama A.,
E., Nakata H., Kujime Y., Inamoto S., Hirose M.,
Nakayama A.: “Fates of Cdh23/CDH23 with mutations
Inabe K., Terashima M., Yamasaki T., Liu B., Nakade
affecting the cytoplasmic region.” Hum Mutat 27, 88-97
K., Pan J., Kimura M., Saito I., Hamada H., Obata Y.,
(2006).*
Yokoyama K.K.: “A database of recombinant viruses
8. Kaneko S., Aki I., Tsuda K., Mekada K., Moriwaki K.,
and recombinant viral vectors available from the RIKEN
Takahata N., Satta Y.: “Origin and evolution of processed
DNA bank.” J Gene Med 7, 1148-1157 (2005).*
pseudogenes that stabilize functional Makorin1 mRNAs
in mice, primates and other mammals.” Genetics 172,
2. Toyo-Oka K., Sasaki S., Yano Y., Mori D., Kobayashi
2421-2429 (2006).*
T., Toyoshima YY., Tokuoka SM., Ishii S., Shimizu
T., Muramatsu M., Hiraiwa N., Yoshiki A., WynshawBoris A., Hirotsune S.: “Recruitment of katanin p60 by
9. Inoue K., Noda S., Ogonuki N., Miki H., Inoue S.,
phosphorylated NDEL1, an LIS1 interacting protein,
Katayama K., Mekada K., Miyoshi H., Ogura A.:
is essential for mitotic cell division and neuronal
“Differential developmental ability of embryos cloned
migration.” Hum Mol Genet 14, 3113-3128 (2005).*
from tissue-specific stem cells.” Stem Cells 25, 12791285 (2007).*
3. Sasaki S., Mori D, Toyo-oka K., Chen A., Garrett-Beal
L., Muramatsu M., Miyagawa S., Hiraiwa N., Yoshiki
10. Ike F., Bourgade F., Ohsawa K., Sato H., Morikawa S.,
A., Wynshaw-Boris A., Hirotsune S.: “Complete loss of
Saijo M., Kurane I., Takimoto K., Yamada Y K., Jaubert
Ndel1 results in neuronal migration defects and early
J., Berard M., Nakata H., Hiraiwa N., Mekada K.,
embryonic lethality.” Mol Cell Biol 25, 7812-7827
Takakura A., Itoh T., Obata Y., Yoshiki A., Montagutelli
(2005).*
X.: “Lymphocytic choriomeningitis infection undetected
by dirty-bedding sentinel monitoring and revealed after
embryo transfer of an inbred strain derived from wild
4. Nakamura K., Suzuki Y., Inoue N., Noro C., Suzuki A.:
mice.” Comparative Medicine 57, 272-281 (2007).*
“Structural characterization of neutral glycosphingolipids
by thin-layer chromatography coupled to matrix-assisted
laser desorption/ionization quadrupole ion trap time-of-
11. Nakade K., Pan J., Yoshiki A., Ugai H., Kimura M.,
Liu B., Li H., Obata Y., Iwama M., Itohara S., Murata
flight MS/MS.” Aral. Chem. 78, 5736-5743 (2006).*
T., Yokoyama KK.: “JDP2 suppresses adipocyte
differentiation by regulating histone acetylation.” Cell
5. Sato J.J., Tsuru Y., Hirai K., Yamaguchi Y., Mekada
Death Differ (2007).*
K., ., Takahata N., Moriwaki K.: “Further evidence for
recombination between mouse hemoglobin beta b1 and
b2 genes based on the nucleotide sequences of intron,
12. Shinmen A., Honda A., Ohkawa M., Hirose M.,
UTR, and intergenic spacer regions.” Genes Genet Syst
Ogonuki N., Yuzuriha M., Miki H., Mochida K., Inoue
81, 201-209 (2006).*
K., Abe K., Ito M., Ogura A.: “Efficient production of
intersubspecific hybrid mice and embryonic stem cells
by intracytoplasmic sperm injection.” Mol Reprod Dev
6. Yoshiki A., Moriwaki K.: “Mouse phenome research:
(2007).*
implications of genetic background.” Ilar J 47, 94-102
(2006).*
13. Tanaka S., Miura I., Yoshiki A., Kato Y., Yokoyama H.,
7. Yonezawa S., Yoshizaki N., Kageyama T., Takahashi
Shinogi A., Masuya H., Wakana S., Tamura M., Shiroishi
T., Sano M., Tokita Y., Masaki S., Inaguma Y., Hanai
T.: “Mutations in the helix termination motif of mouse
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type I IRS keratin genes impair the assembly of keratin
16. Hirano T., Ike F., Murata T., Obata Y., Uchiyama H.,
intermediate filament.” Genomics 90:703-711 (2007).*
Yokoyama K.: “Genes encoded within 8q24 on the
amplicon of a large extrachromosomal element are
14. Endoh K., Mochida K., Ogonuki N., Ohkawa M. “The
selectively repressed during the terminal differentiation
developmental ability of vitrified oocytes from different
of HL-60 cells.” Mutation Research 640, 97-106 (2008).*
mouse strains assessed by parthenogenetic activation
and intracytoplasmic sperm injection.” Journal of
17. Ta k im o to K ., Ta h a ra g u c h i M ., Ik e F., Ya m ada
Reproduction and Development 53,1199-1206 (2007).*
Y.: “Detection of the antibody to lymphocytic
choriomeningitis virus in sera of laboratory and newly
15. Toyo-oka K., Mori D., Yano Y., Shiota M., Iwao H., Goto
isolated strains by ELISA using purified recombinant
H., Inagaki M., Hiraiwa N., Muramatsu M., Wynshaw-
nucleoprotein.” Experimental Animals 57, No.4, 357-365
Boris A., Yoshiki A., Hirotsune S.: “Protein phosphatase 4
(2008).*
catalytic subunit regulates Cdk1 activity and microtubule
organization via NDEL1 dephosphorylation.” J Cell Biol
18. Motokawa M., Harada M., Mekada K., Shrestha KC.:
180: 1133-1147 (2008).*
“Karyotypes of Soriculus nigrescens and Episoriculus
caudatus from Nepal (Soricomorpha, Soricidae).”
Integrative Zoology (in press).*
Oral Presentations
【International Conferences】
1. Yoshiki A., Mekada K., Nakata H., Hiraiwa N., Ike F.,
Mochida K., Moriwaki K., Obata Y.: “Establishment of
5. Mise N., Fuchikami T., Sugimoto M., Kobayakawa
mouse strain resources in RIKEN BioResource Center.”
S., Yuzuriha M., Ike F., Tada T., Ogawa T., Kanaya S.,
International Symposium of the Korean Association for
Noce T., Abe K.: “Classification of embryo-derived stem
Laboratory Animal Science, Seoul, Korea, Jun. (2005).
cells and germ cells by genome-wide gene expression
profiling.” International Symposium on Germ Cells,
2. Satoko K., Tsuda K., Mekada K., Moriwaki K., Takahata
N., Satta Y.: “Rapid turnover of regulatory processed
Epigenetics, Reprogramming and Embryonic Stem Cells,
Kyoto, Japan, Nov. (2005).
pseudogenes of Makorin1 in rodents and primates.”
Molecular Biology & Evolution 2005, Auckland, New
Zealand, Jun. (2005).
6. Sugimoto M., Mekada K., Karashima Y., Yuzuriha M.,
Ko S. H. M., Nagaraja, Tan S. S., Takagi N., Abe K.:
“Narrowing down the position of the t-complex recessive
3. Yoshiki A.: “The RIKEN BioResource Center in the
lethal mutation tclw5 into 180kb by BAC rescue.” 19th
International Network of Mouse Strain Resources.”
International Mouse Genome Conference, Strasbourg,
Korean Society for molecular and cellular biology
France, Nov. (2005).
meeting, Seoul, Korea, Sep. (2005).
7. Mekada K., Arai F., Murakami A., Oota S., Moriwaki
4. Fuchikami T., Mise N., Sugimoto M., Kobayakawa S.,
K., Obata Y., Yoshiki A.: “Analysis of a new recessive
Kondo M., Ike F., Abe K.: “Dynamics of global gene
mutant with abnormal walking.” 19th International
expression changes during mouse primordial germ cell
Mouse Genome Conference, Strasbourg, France, Nov.
development.” International Symposium on Germ Cells,
(2005).
Epigenetics, Reprogramming and Embryonic Stem Cells,
Kyoto, Japan, Nov. (2005).
8. Mise N., Fuchikami T., Sugimoto M., Kobayakawa
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S., Yuzuriha M., Ike F., Tada T., Ogawa T., Kanaya S.,
“Differential developmental ability of embryos cloned
Noce T., Abe K.: “Classification of embryo-derived stem
from tissue-specific stem cells.” The 33rd Annual
cells and germ cells by genome-wide gene expression
Conference of International Embryos Transfer Society,
profiling.” 20th IUBMB International Congress of
Kyoto, Japan, Jan. (2007).
Biochemistry and Molecular Biology and 11th FAOBMB
Congress, Kyoto, Japan, Jun. (2006).
16. Nakamura K., Suzuki Y., Goto-Inoue N., YoshidaNoro C., Suzuki A.: “TLC-MALDI-MS for Neutral
9. Doi T., Mise S., Hiraiwa N., Ike F., Yoshiki A., Kasai
Glycosphingolipids”, Glycobiology and Sphingobiology
K., Obata Y.: “Transcription factor NF-kB is essential at
2007 (GS2007): Hakomori Commemorative Forum ,
neonatal stage.” 20th IUBMB International Congress of
Tokushima, Feb. (2007).
Biochemistry and Molecular Biology and 11th FAOBMB
Congress, Kyoto, Japan, Jun. (2006).
17. Ike F., Aoki H., Morikawa S., Yoshiki A., Yamagata Y.:
“Identification of mouse antibody reaction by multi-
10. Ike F., Yoshiki A.: “Lymphocytic choriomeningitis virus
channel microfluidic immunoassay chip using small
(LCMV), re-emerging pathogen.” 2nd AALAS Congress
amounts of diluted serum.” FELASA-ICLAS Joint
in 2006, Jeju, South Korea, Aug. (2006).
Meeting 2007, Cernobbio, Italy, Jun. (2007).
11. Mise N., Yuzuriha M., Kondo M., Ike F., Araki K., Tada
18. Ike F., Kajita A., Aoki H., Kase H., Nagamune T.,
T., Ogawa T., Kanaya S., Noce T., Abe K.: “Classification
Morikawa S., Obata Y., Yamagata Y.: “Detection of
and characterization of ES, EG and primordial germ
emerging zoonotic infection in mice by high sensitive
cells having different sex chromosome compositions by
multiplexed microfluidic immunoassay system.” 11th Int.
microarray-based expression profiling.” 2nd International
Conf. on Miniaturized Systems for Chemistry and Life
Conference on X-Inactivation, Paris, France, Sep. (2006).
Sciences (μTAS 2007), Paris, France, Oct. (2007).
12. Moriwaki K., Ike F., Takakura A.: “Establishment
19. Aoki H., Kajita A., Kaneko A., Ishihara M., Hara T.,
of detection system for LCMV infection in RIKEN
Nonaka H., Yamagata Y., Ike F., Kase H.: “Development
BioResource Center and CIEA.” US Japan meeting 2006,
of new rapid multiplex microfluidic chip system for
Salt Lake City, USA, Oct. (2006).
research animal serology monitoring.” 58th AALAS
National Meeting, Charlotte, USA, Oct. (2007).
13. Fuchikami T., Mise N., Sugimoto M., Kobayakawa
S., Kondo M., Ike F., Abe K.: “Dynamics of global
20. Ike F., Kajita A., Yoshiki A., Obata Y., Aoki H., Kase
gene expression in primordial germ cells during mouse
H., Yamagata Y., Morikawa S.: “Serological profiling
development.” Cold Spring Harbor Laboratory 2006
of lymphocytic choriomeningitis infected wild-derived
Meeting on Germ Cells, Cold Spring Harbor, USA, Oct.
mice.” 58th AALAS National Meeting, Charlotte , USA,
(2006).
Oct. (2007).
14. Nakata H., Kitaura Y., Mekada K., Murakami A., Obata
21. Tanaka S., Miura I., Yoshiki A., Kato Y., Yokoyama H.,
Y., Yoshiki A.: “The RIKEN BioRessource Center to
Shinogi A., Masuya H., Wakana S., Tamura M., Shiroishi
disseminate the highest quality mouse resource for
T.: “Abnormal Assembly of Keratin Intermediate
biomedical research.” Super Computing 2006, Florida,
Filaments in Mouse Mutations of Type I Inner Root
USA, Nov. (2006).
Sheath Keratin.” 21st International Mammalian Genome
Conference (IMGC2007), (IMGC), Kyoto, Oct.-Nov.
15. Inoue K, Ogonuki N., Miki H., Noda S., Inoue S.,
Katayama K., Mekada K., Miyoshi H., Ohura A.:
― 48 ―
(2007).
22. Araki K., Takeda N., Yoshiki A., Yamada G., Nakagata N.,
Kitaura Y., Mochida K., Kadota M., Murakami A., Fujita
Shiroishi T., Moriwaki K., Yamamura K.: “Establishment
M., Ohkawa M., Ogura A., Abe K., Moriwaki K., Obata
of embryonic stem cell lines derived from Msm/ms
Y.: “RIKEN BRC to establish mouse resources of the
strain originated from mus musculus molossinus.”
highest global standards.” 21st International Mammalian
21st International Mammalian Genome Conference
Genome Conference (IMGC2007), Kyoto, Oct.-Nov.
(IMGC2007), Kyoto, Oct.-Nov. (2007).
(2007).
23. Oota S., Mekada K., Arai F., Obata Y., Fukami K.,
29. Mekada K., Abe K., Murakami A., Nakamura S., Obata
Yoshiki A.: “Four-Dimensional quantitative analysis
Y., Yoshiki A.: “Which C57BL/6 substrain is used for
on the gait of mutant mice by using the motion capture
the background strain of your mouse?” 21st International
technology.” 21st International Mammalian Genome
Mammalian Genome Conference (IMGC2007), Kyoto,
Conference (IMGC2007), Kyoto, Oct.-Nov. (2007).
Oct.-Nov. (2007).
24. Sato J. J., Yamaguchi Y., Ueta J., Suzuki H., Chunyan W.,
30. Yoshiki A., Ike F., Hiraiwa N., Nakata H., Mekada K.,
Kryukov A. P., Mekada K., Takahata N., Moriwaki K.:
“Genetic characterization of the Wild-derived house mice
M., Okawa M., Ogura A., Abe K., Moriwaki K., Obata
in east asia.” 21st International Mammalian Genome
Y.: “The highest quality mouse resources for global
Conference (IMGC2007), Kyoto , Oct.-Nov. (2007).
biomedical researchers from Riken BRC.” Satellite
Meeting. The 10th Annual Meeting of Chinese-Taipei
25. Miura I., Tanaka S., Yokoyama H., Shinogi A., Kobayashi
Society of Laboratory Animal Science. Taipei, Dec.
K., Kaneda H., Toyoda T., Yoshiki A., Yonekawa H.,
(2007).
Kikkawa Y., Kunieda T., Tamura M., Masuya H., Wakana
S., Shiroishi T.: “Hair Morphological Mutants Generated
in the RIKEN Mutagenesis Project.” 21st International
Mammalian Genome Conference (IMGC2007), (IMGC),
M., Ogura A., Abe K., Wakana S., Moriwaki K., Obata
Kyoto, Oct.-Nov. (2007).
Y.: “Distribution of the highest quality mouse resources
from Riken BioResource Center.” The 6th International
26. Yokota H., Kakusho N., Mekada K., Murakami A.,
Conference on Bioinformatics of Genome Regulation
Nakamura S., Ito M., Kase K., Oota S., Fukami K.,
and Structure, Novosibirsk, Jun. (2008).
Masuya H., Wakana S., Moriwaki K., Shiroishi T.,
Himeno R., Obata Y., Yoshiki A.: “High-Resolution
32. Mekada K., Murakami A., Abe K., Moriwaki K., Obata Y.,
full color digital mouse anatomy by three-dimensional
Yoshiki A.: “Genetic variation of mouse inbred strain”,
internal structure microscopy.” 21st International
The 6th International Conference on Bioinformatics of
Mammalian Genome Conference (IMGC2007), Kyoto,
Genome Regulation and Structure, Novosibirsk, Jun.
Oct.-Nov. (2007).
(2008).
27. Kitaura Y., Mekada K., Nakata H., Matsushima Y., MeiLing J., Shiroishi T., Moriwaki K., Obata Y., Yoshiki
【Domestic Conferences】
Total 68
A.: “Immunological profiling of laboratory inbred
and wild-derived mouse strains developed in Japan.”
21st International Mammalian Genome Conference
(IMGC2007), Kyoto, Oct.-Nov. (2007).
― 49 ―

Experimental Animal Division

Transcription

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