Muncan Poster - Water Journal
Transcription
Muncan Poster - Water Journal
It is for some time known that paramagnetic/diamagnetic properties of matter, including water could be measured (Fig.1a), and that these are a result of complex dynamics – constant making and breaking of water clusters [1]. The method of Optomagnetic spectroscopy introduced by Koruga et. al [2,3] is based on the polarization properties of water and in result gives a summarized magnetic properties (paramagnetic and diamagnetic) of the sample (Fig1b) [3], among other useful information. Thus, Opto-magnetic spectroscopy was shown to be a valuable tool with great potential in various biomedical and other applications [3-5]. Aquaphotomics is a scientific method which gives insight into water organization through specific “water-mirror” approach, using near infrared light [6]. Using near infrared spectroscopy and Aquaphotomics it is possible to observe and analyze water molecular vibrations in relation to other molecular vibrations and function of the whole analyzed system under various perturbations [6,7]. So, what happens to water and its magnetic properties in cancerous tissues? Or what happens to water exposed to light? Or plants in dark and light ambient? How anesthetics change para/diamagnetic properties of tissues? How water reacts to the words spoken to it? And does water really remember? C9H13NO3 Ficus Benjamina By cell phone 15 10 Lbefore 120 140 160 180 200 L-1h D-1h Water -10 -15 Wavelength difference [nm] [R-B]&[W-P] 100 50 0 100 θ=53°, Brewster angle RGB 40 Reflected polarized light Electric component Relative intensity x 1000 [n.a.u. ] Reflected white light Electromagnetic component 50 0 100 105 0 100 105 110 115 120 125 130 135 140 Magnet1 Magnet2 Magnet3 -20 115 120 125 130 -100 100 20 110 -50 Adrenalin ampula 1 1000 PLACEBO 0 100 110 120 130 -100 WAVELENGTH DIFFERENCE [nm] 100 110 120 130 -50 WAVELENGTH DIFFERENCE [nm] 105 110 115 120 125 -100 WAVELENGTH DIFFERENCE [nm] 0 100 130 120 130 -100 WAVELENGTH DIFFERENCE [nm] Adrenalin 6C PLACEBO 100 50 50 0 100 110 120 130 0 100 105 110 115 120 125 130 -50 -50 -100 110 -50 WAVELENGTH DIFFERENCE [nm] 100 0 100 50 Adrenalin C200 TEL Adrenalin 200C PLACEBO 100 -50 -50 0 -100 50 50 50 -100 WAVELENGTH DIFFERENCE [nm] WAVELENGTH DIFFERENCE [nm] Wavelength difference [nm] NIRS & Aquaphotomics– selected results [Red Channel – Blue Channel]& [White – Polarized] RGB 130 Adrenalin C200 100 100 WAVELENGTH DIFFERENCE [nm] -40 RGB 120 -100 INTENSITY (n.a.u. x 1000) Intensity x 1000 [n.a.u.] θ=90° 110 -50 60 Method I Opto-Magnetic Imaging Spectroscopy (OMIS) Adrenalin C6 Adrenalin ampulla 1:1000 100 INTENSITY (n.a.u. x 1000) 100 INTENSITY (n.a.u. x 1000) -5 Opto-Magnetic Imaging Spectroscopy (OMS) – selected results Dbefore 0 INTENSITY (n.a.u. x 1000) 5 INTENSITY (n.a.u. x 1000) Fig.1b) Opto – magnetic spectra of pure water Fig.1a) Paramagnetism/Diamagnetism of water By thought INTENSITY (n.a.u. x 1000) Wavelength difference [nm] 20 INTENSITY (n.a.u. x 1000) [R-B]&[W-P] By electrical device (workshop) INTENSITY (n.a.u. x 1000) Realitve Intensity x 000 [n.a.u] Relative intensity x 1000 [n.a.u. ] [R-B]&[W-P] - average spectra of plant leaves Adrenalin C6 (homeopathic solution) and Adrenalin ampulla 1:1000 aquagrams are very different comparing to other aquagrams. Wavelength difference [nm] Fig.2. Principles of Opto-Magnetic Imaging Spectroscopy Method II Near Infra Red Spectroscopy (NIRS) &Aquaphotomics Mucose White Polarized NIR spectrometer: Hamamatsu TG 9113GC (Hamamatsu, OMIS: Water has a characteristic opto-magnetic spectrum, Adrenalin has its own opto-magnetic spectrum, different to water. Homeopathic solutions C6 and C200 have spectra similar to both substances – water and adrenalin. Placebo solutions (pure water) have different spectra comparing to water, and in some cases similar to the Adrenalin Japan) Sample cell: quartz cell, path length 1mm NIR water spectrum Mode: Transmittance Cancer Aquagram White Polarized NIRS & AQUAPHOTOMICS: Results show that the Adrenalin ampulla 1:1000 and Adrenalin C6 homeopathic differ strongly comparing to other solutions. When excluded from comparative aquagrams, the rest of the solutions show similar organization of water molecules, where only concentration of typical water molecular species varies. [R-B]&[W-P] – averaged spectra for 34 patients Relative Intensity x 1000 [n.a.u.] 15 Fig.3 Basic principles of Aquaphotomics and an example of aquagram derived from NIR water spectrum 10 5 0 100 110 120 130 -5 -10 Wavelength difference [nm] Mucose Cancer 140 150 1) Paramagnetic/Diamagnetic (P/D) property of water may be determinate by proton magnetometers based on Overhauser effect (measuring changes in nT) and by Opto-Magnetic Imaging Spectroscopy- OMIS (qualitative method giving relative P/D change in normalized arbitrary units, during light-water interaction). 2) Since those two methods are compatible and complementary we used OMIS to characterize skin, plants, mucosa tissue of colon and different drug types of medicines, including placebos 3) Applying anesthetics on skin leads to diminished paramagnetic properties 4) After exposure to sun, paramagnetism of plants’ leaves is increased 5) Paramagnetic properties of adenocarcinoma tissues are decreased comparing to healthy mucosa tissue of colon 6) OMIS diagrams of paramagnetic/ diamagnetic properties of water, adrenalin, homeopathic solutions of adrenalin and different placebos are different. This is also confirmed by using Aquaphotomics. These are very preliminary results and more research is necessary for final conclusion. Relative intensity x 1000 [n.a.u] [R-B]&[W-P] 15 10 1) Koruga ,D., Nikolic,A., Mihajlovic,S., Matija L., Nanomagnetic behavior of fullerene thin films in Earth magnetic field in dark and under polarization light influences, J. Nanoscience and Nanotechnology, Vol.5. No 10, 1660-1664 (2005) 2) Dj. Koruga, A. Tomic, System and method for analysis of light - mater interaction based on spectral convolution, US Patent application number 20090245603, (2009 ) 3) Dj. Koruga, S. Miljkovic, S. Ribar, L. Matija, D. Kojic, Water hydrogen bonds studied by opto-magnetic fingerprint technique, Acta physica polonica A, Vol.117 -5 (2010) 777-781 4) Papić-Obradović, M, Kojić,D., Matija,L., Opto-Magnetic Method for Epstein-Barr Virus and Cytomegalovirus Detection in Blood Plasma Samples, Acta Physica Polonica A,Vol.117 (5), pp. 781-784, 2010 5) Jelena Muncan, A comparative study on structure and properties of water by opto-magnetic and IR spectroscopy, Contemporary materials, Vol.III1, 2012 (ISSN: 1986-8669) (proof of acceptance No. 04/3-121/12) 6) R.Tsenkova, Introduction to Aquaphotomics: dynamic spectroscopy of aquaous and biological systems descibes peculiarities of water. Journal of Near Infrared Spectroscopy, 2009. 17: p. 303-314. 7) Matija,L., Tsenkova,R., Miyazaki,M., Bamba, K, Munćan, J., Aquagrams: Water spectral pattern as characterization of hydrogenated nanomaterial, FME Transaction,Vol.40, No.2, 51-56, 2012. (ISSN:1451-2092) 5 0 100 105 110 115 120 125 130 -5 -10 Wavelength difference [nm] Before After This research has been partially funded by Ministry of Science and Technological Development of Republic of Serbia, through Projects III 41006 and III 45009 Jelena Muncan, Djuro Koruga,