Peter Leendertse
Transcription
Peter Leendertse
Azole fungicides, aspergillosis and preliminary ‘hot spots’ Peter Leendertse CLM (Centre for Agriculture and Environment) IATP webinar 5 March 2015 Erna van der Wal (CLM) Wouter van der Weijden (CLM) en Paul Verweij (Radboud UMC) 1 Resistant fungi: new risk for humans • In NL lots of attention on human health and antibiotic resistance, including measures • Little attention on human health and fungicide resistance Verweij: Doden door agressieve infecties met resistente schimmels, NOS, 17-06-2010 Van der Wal: Resistente schimmels zijn tijdbom, Nieuwe Oogst 7 maart 2012 Aspergillus fumigatus 2 3 4 5 6 Human problem 7 Where do these azole-resistant strains originate? 8 9 10 Where do these azole-resistant strains originate? 1. Where do we find Aspergillus? 2. Where are azoles used? 3. What azole amounts are used? 4. Where are optimal conditions for development of restistance in Aspergillus? 11 1. Where do we find Aspergillus? • • • • • • • • • • • Air Animals Compost Farms Humans Houses Hospitals Nursery Plants Soil Water Everywhere! 12 2.Where are azoles used? Everywhere! • • • • • • • • Human and animal protection (medicines) Animal protection (biocides) Crop protection (pesticides) Fruit conservation (pesticides) Wood conservation (biocides) House protection (wall paper paste, sealants) Film conservation (biocides) Many other protection or conservation 13 3. What azole amounts are used? Total Agriculture NL: >130.000 kg yearly in > 50 crops Fungicides Use (kg) Area(ha) Crops with highest use Prothioconazool 40.000 152.000 Winter wheat, tulip, lily, onion Epoxiconazool 25.000 100.000 Winter wheat, sugar beet Tebuconazool 35.000 125.000 Winter wheat, tulip, lily, grass seed Cyproconazool 3.600 26.000 Sugar beet, winter wheat, Difenoconazool 8.500 40.000 sugar beet, cabbage, apple & pear 800 14.000 Winter wheat 1.700 3.200 Metconazool Propiconazool Pot plants, trees, flowers (CLM estimates based on CBS 2012 and expert judgement), Official numbers still secret 14 3. What azole amounts are used? Total other uses NL: • In Dutch healthcare: 400 kg of azoles annually • Other uses: unknown 15 Remarkable observations Promotion of azoles in maize Quilt® Xcel (propiconazole/azoxystrobin) effectief tegen schimmelziekten Maïs langer groen en gezond Het nieuwe fungicide Quilt Xcel houdt maïs langer gezond en vitaal. Quilt Xcel biedt telers daarom meer zekerheid en rust. !promotion for azole even without fungus problems in maize 16 4. Where are optimal conditions for resistance development? Not everywhere! • • • • • Presence of Aspergillus Azole pressure High moisture High temperature Presence of oxygen Let’s search for “Hot spots” 17 Preliminary results in search of ‘hot spots’ Composting material a-sexual sexual resistant a-sexual resistant sexual 1. No azole resistant strains left after composting process 2. Sexual strain not reproducible Compost 18 Peel waste of bulbs Preliminary results in search of ‘hot spots’ a-sexual Conventional farming (prothioconazool 10mg/kg) sexual resistant a-sexual Resistant sexual Ecological farming (no fungicides) 19 Indications of the preliminary exploration Few samples & few replications! • Aspergillus in different predicted locations (compost, peel waste of bulbs) • Both sensitive and resistant strains • Sometimes only resistant strains • Sometimes hardly Aspergillus (dry straw of wheat, ecological farming) 20 How to reduce the risk of fungicide resistance? Focus in four steps! 1. Identify “hot spots” 2. Analyse mechanisms 3. Eliminate “hot spots” -change methods for plant waste material -reduce azole use -stimulate alternatives, preferably methods with less or no induction of resistance. 4. Cooperate internationally and advise registration authorities 21 Findings conference KNAW (Royal Dutch Academy of Sciences) 2-4 March 2015 22 Findings conference KNAW 23 Findings conference KNAW 24 Findings conference KNAW 25 Sustainable farming – Healthy food – A living countryside Thank you very much for your attention! Any questions?