Model S2 - LABRepCo

www.biometra.com
Model S2
Sequencing Gel Electrophoresis System
• Produces a 30 cm x 40 cm gel that fits easily on a standard 14 in x 17 in
X-ray film
• Integral aluminum plate for even heat
distribution to minimise band curvation
(“smiling”)
• Removable lower buffer tray with a
separate chamber for collecting the
buffer from the upper reservoir,
eliminating the need to move the
apparatus for buffer disposal
• Built-in integral clamps hold the gel
in place
• Unique gasket sealing system ensures
leak-free operation without grease or
notched plates
• Safety interlock lids
• Additional combs and apparatus
components available separately
The Model S2 Sequencing Gel
Electrophoresis Apparatus is designed for
vertical polyacrylamide gel separations
of oligo- and polynucleotides, nucleic
acid sequencing, genotyping and
polymorphic studies.
Gel Casting Clamp
Apparatus dimensions (W x D x H)
Gel dimensions (W x H)
42.2 cm x 21.6 cm x 44.5 cm
31.0 cm x 38.5 cm
Working buffer volume: 1 L
Item
Order No.
Model S2
21105010 (USA)
Complete with power cords, four 14 cm 0.4 mm vinyl sharktooth
21105036 (EU)
combs, set of 0.4 mm thick vinyl side spacers; pair of glass plates, roll
of gel sealing tape; surface temperature monitor
Model S2 including gel casting clamp (21105432)
21105499 (USA)
21105481 (EU)
DNA sequence analysis
performed on the Model
S2 System.
8 % polyacrylamide/8.3 M
urea gel Tris-borate-EDTA
buffer electrophoresed at
1.5 kV for 2.5 h.
70
Ordering Information for Accessories
see page 72 - 73
Elect rophoresis Sequencing
Model SA
Adjustable Sequencing Gel Electrophoresis System
• Snap-action section clamps allow
quick and easy adjusting of the height
• Adjustable levelling feet and built-in
levelling indicator ensure consistent
and reproducible results
• Integral aluminium plate for even heat
distribution to minimise “smiling”
• Removable lower buffer tray with
a separate chamber for collecting the
buffer from the upper reservoir
eliminating the need to move the apparatus for buffer disposal
• Built-in integral clamps hold the gel
in place
•Unique gasket sealing system ensures
leak-free operation
• Safety interlock lids
•Combs and apparatus components
available
The Model SA sequencing apparatus
is designed for the separation of
oligonucleotides, polynucleotides and
nucleic acid sequences in vertical
polyacrylamide gels. With optional
extensions the unit can easily be
adjusted to three different heights:
32 cm, 43 cm or 60 cm.
Model
Apparatus dimensions (W x D x H)
Gel dimensions (W x H)
SA-32
29 cm x 22 cm x 41 cm
17 cm x 32 cm
SA-43
29 cm x 22 cm x 52 cm
17 cm x 43 cm
SA-60
29 cm x 22 cm x 68 cm
17 cm x 60 cm
Working buffer volume: 500 ml
Item
Order No.
Model SA-32
31096019 (USA)
Complete with top and base sections, power cords; two 14 cm vinyl
31096027 (EU)
sharktooth combs; set of 0.4 mm thick spacers; pair of glass plates; roll
of gel sealing tape; surface temperature monitor.
Model SA-43 (one extension)
21105499 (USA)
21105481 (EU)
Model SA-60 (one extension)
31096035 (USA)
31096043 (EU)
Gel Casting Clamp
31096324
SA Extension (27 cm)
31096076
SA Extension (11 cm)
31096423
DNA sequence analysis performed on the Model
SA-60 System.
GEL-MIX 6, 6 % SDS-polyacrylamide gel
Tris-borate-EDTA buffer electrophoresed at 55 W
constant power (2.3 to 2.7 kV) for 3 h.
Ordering Information for Accessories
see page 72 - 73
71
www.biometra.com
Combs for Sequencing Apparatus
Shark tooth combs
•The teeth form barriers between
adjacent lanes for sample loading
• Reduced risk of torn or deformed wells
cause by square-toothed combs
•The minimal space between lanes
facilitates accurate reading of sequences
• Generate flatter, more uniform loading
surface than square-toothed combs
Shark tooth combs are ideally suited for
facilitating the analysis of sequencing
reactions. They are designed for use
with all sequencing apparatus models
(S2, S2001 and SA). In addition, the
combs can be used with all other
systems with 0.4 mm spacers.
Square tooth combs for model S2001 and S2
Number of wells
Comb thickness
Volume/well
Order No.
16
0.4 mm
25 µl
21035043
16
0.8 mm
50 µl
21035050
16
1.6 mm
100 µl
21035068
20
0.4 mm
19 µl
21035076
20
0.8 mm
38 µl
21035084
20
1.6 mm
76 µl
21035092
32
0.4 mm
10 µl
21035100
32
0.8 mm
20 µl
21035118
32
1.6 mm
40 µl
21035126
Sample application.
Shark tooth combs for all models
72
Comb length
Comb thickness
Number of teeth
Volume/well
Pcs./Pckg.
Order No.
14 cm
0.4 mm
25
2 – 4 µl
6
21045018
28 cm
0.4 mm
50
2 – 4 µl
2
21046016
14 cm
0.4 mm
49
1 – 2 µl
4
21045026
14 cm
0.19 mm
25
2 – 4 µl
6
21105317
14 cm
0.35 mm
25
2 – 4 µl
6
21105341
28 cm
0.35 mm
62
2 – 4 µl
2
21035134
Elect rophoresis Sequencing
Combs for Sequencing Apparatus
Order Information
Square tooth combs for model SA
Number of wells
Comb thickness
Volume/well
Order No.
3
0.4 mm
240* µl
11092012
3
0.8 mm
480* µl
11092020
3
1.6 mm
960* µl
11092038
12
0.4 mm
15 µl
11092061
12
0.8 mm
30 µl
11092079
12
1.6 mm
60 µl
11092087
18
0.4 mm
8 µl
11092095
18
0.8 mm
16 µl
11092103
18
1.6 mm
32 µl
11092111
* Central, preparative well
Accessories
Item
Dimensions
Content
Order No.
Spacer set for Model S2001/S2
0.19 mm
2 Spacer, 12 End blocks
21105309
Spacer set for Model S2001/S2
0.35 mm
2 Spacer, 12 End blocks
21105366
Spacer set for Model S2001/S2
0.4 mm
2 Spacer, 12 End blocks
21108014
Spacer set for Model S2001/S2
0.8 mm
2 Spacer, 12 End blocks
31109010
Spacer set for Model S2001/S2
1.6 mm
2 Spacer, 12 End blocks
31109028
Wedge spacer for Model S2001/S2
0.4 – 1.2 mm 2 Spacer
21107016
Wedge spacer for Model SA-32
0.4 – 1.2 mm
2 Spacer
21093083
Wedge spacer for Model SA-60
0.4 – 1.2 mm
2 Spacer
21093133
End spacer for Model S2001/S2
0.4 mm
1 Spacer
21105077
End spacer for Model S2001/S2
0.8 mm
1 Spacer
21105069
End spacer for Model S2001/S2
1.2 mm
1 Spacer
21105325
End spacer for Model S2001/S2
1.6 mm
1 Spacer
21105051
12 Pieces
21965017
Spacer silicon blocks
12 Pieces
21105382
Glass plate set for S2001/S2
33 cm x 39 cm
2 Plates
11034014
Glass plate set for SA-32
19.7 cm x 33.8 cm
2 Plates
21093067
Spacer foam blocks
Glass plate set for SA-60
2 Plates
21093117
Gel casting clamp for S2001/S2
1 Piece
21105432
Gel casting clamp for SA-32
1 Piece
31096324
Gel sealing tape
1 Roll
11032018
Gel sealing tape
10 Rolls
11032026
Sealing for buffer chamber S2001/S2
1 Piece
21105358
Sealing for buffer chamber SA
1 Piece
21093034
Top clamp
1 Piece
21047014
Clamps for gel sandwich
12 Pieces
11098019
Clamps for gel sandwich (stainless)
12 Pieces
21105333
Surface temperature monitor strips
3 Pieces
21105358
73
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Gel Casting Clamps
• Easy assembly and clean up
• Provides a tight, leak-free seal and
even pressure over the entire surface
•Cast gels from 0.2 to 0.8 mm thickness
and wedge gels from 0.4 to 1.2 mm
thickness
• Gels can be cast from the top or
through the bottom port using a
syringe or gradient former.
The S2/2001 and SA gel casting clamp
provides an easy way to cast sequencing
gels without the use of tape or binder
clamps.
74
Model
Glass plate (W x H)
Gel (W x H)
Order No.
Casting clamp for
S2/2001
33.0 cm x 39.0 cm
31.0 cm x 39.0 cm
21105432
Casting clamp for SA
19.7 cm x 33.8 cm
17.0 cm x 33.8 cm
31096324
Elect rophoresis Sequencing
75