mini catalog_NEW - FOTODYNE Educational Products Division

FOTODYNEMolecularBiologyforYourClassroom
AP Biology
Big Idea 1
FOTODYNE2015StudentArtContestWinner:
Evolution+Diversity=Life
byMaciGarcia
JohnBurroughsHighSchool
Burbank,CA
EmpoweringTomorrow’sScientists
At FOTODYNE, our goal is to provide
Our MolecuLab teaching kits allow
opportunities for your students to think
students to use technology to develop
and feel like scientists. When your
critical thinking skills while focusing
students use our gel boxes, power
on biological themes and big ideas.
supplies, transilluminators, and camera
These kits are designed and written with
systems, they work with the same
assistance from teachers to help provide
caliber equipment encountered in real
thought-provoking, hands-on laboratory
biotechnology research settings.
experiences that are mindful of important
educational standards like NGSS and
AP® Biology.
Please see www.fotodyneclassroom.com to learn more about our products.
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E-mail:[email protected]•Web-site:www.fotodyneclassroom.com
FOTODYNEIncorporated•950WalnutRidgeDrive•Hartland,WI53029-9388•USA
Featured Kits & Equipment
Theme:
The process
of evolution
drives the
diversity and
unity of life.
DurableClassroomEquipment
Constructed for durability, FOTODYNE
equipment stands up to direct contact with
tens of thousands of students year after
year. In fact, many schools are still using
the same instruments they purchased from
us more than 20 years ago.
“Dear FOTODYNE . . .
I am still using my 20 year old Fotodyne gel rigs and power supplies.
They have been used by countless students in classes and teachers
in workshops over the years and have now been “retired” to my
basement lab where they continue to be as reliable as ever. In those
20+ years, I have only had one issue, which your customer service
quickly and willingly solved. Thank you for an outstanding product!”
Mini Single Cell
Our Mini Single Cell allows you to pour leak-free
mini gels in a unique UV-transparent gel tray that
requires no messy taping. After electrophoresis,
the gel tray can be perfectly positioned over the
viewing surface of the FOTO/Phoresis UV or
white light transilluminator for visualization and
photography.
The molded electrophoresis chamber requires
only 150 ml of buffer and is made of sturdy,
clear acrylic, allowing viewing of the run while
in progress. The chamber is also constructed
with convenient holders for storage of combs
when not in use. An interlocking safety lid with
attached leads ensures that power to the gel is
disconnected when the lid is removed, preventing
accidental electrical exposure. Platinum
electrodes provide a uniform, corrosion-resistant
electrical field.
FOTO/Force 300 Power Supply
The FOTO/Force 300 features four sets of colorcoded output jacks - two on the left and two on the
right.
The power supply offers the flexibility of
continuously adjustable voltage selection from 10
to 300 true DC Volts. The lower voltage limit of 10
Volts is perfect for running gels overnight.
The output can be monitored in either milliamps or
volts on the digital display with the flip of a switch.
For safety, the FOTO/Force 300 is built with
overcurrent protection, short circuit protection, and
earth ground isolation.
Furnishing your
teaching lab all at
once?
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• 1/4” clear acrylic chamber
• Interlocking safety lid
• Platinum electrodes
• One (1) stand-alone gel tray
- No taping required
- Pour gel on any level surface
- UV transparent
- 7.2 x 9.3 cm
• Four (4) combs:
6-, 8-, 10-, and 12-well
Ordering information:
E1-1408....................................... $305
Features:
• Four sets of output jacks - two sets
on opposing sides
• Voltage:
10 to 300 DC Volts
• Current:
300 milliamps maximum
• Power:
45 Watts at maximum voltage and
current
• Instruction manual
Ordering information:
E1-3300....................................... $515
Check out our
complete
workstations
online.
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Features:
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E-mail:[email protected]•Web-site:www.fotodyneclassroom.com
DurableClassroomEquipment
FOTO/Phoresis UV Transilluminator
The FOTO/Phoresis UV Transilluminator is
ideal for safe visualization and photography of
DNA mini-gels without leaving the lab bench. A
UV-blocking, safety interlocking cover makes it
possible to safely view the gel without the use of
UV blocking eyeglasses.
Ordering information:
E1-1430........................... $815
For fool-proof photography, the FOTO/Phoresis is
built with an acrylic frame which perfectly positions
your gel in the UV light path. This frame also holds
the FOTO/Phoresis photographic hood in place.
In addition, the acrylic frame has been designed
to accommodate a standard, 100 mm Petri dish
for GFP plates, UV mutagenesis experiments and
other applications.
FOTO/Phoresis White Light
Transilluminator
The FOTO/Phoresis White Light uses colorcorrected white light to enhance visualization of
Coomassie blue-stained protein mini-gels, and
colorimetric reactions in microtiter plates.
Ordering information:
E1-1700........................... $290
Compact and easy to use, the FOTO/Phoresis is
built with a white acrylic viewing surface that is
compatible with the FOTO/Analyst
FOTO/Phoresis hood mount imaging systems
for mini gels. A groove in the viewing surface
facilitates proper placement of your gel or
standard Petri dish for photographing.
Features:
• 8.6 x 10.8 cm viewing surface
• UV Blocking cover with interlocking
safety switch and indicator light
• Perfect positioning of mini-gels for
photodocumentation
• Notched acrylic viewing frame for
holding standard Petri plates
• Two 9-watt, 312 nm bulbs
• Cooling fan
• Instruction manual
Features:
• 7.6 x 10 cm viewing surface
• Two 9-watt twin tube color- corrected
white light bulbs
• Instruction manual
TurnYourCameraPhoneortabletIntoanAffordableGelDocumentationSystem
Discovery/Doc
If you or someone in your classroom has a smart
phone or tablet, you can easily transform it into
an inexpensive gel documentation system. The
FOTODYNE Discovery/Doc™ will allow your
students to consistently capture gel images that
are centered and filtered properly to maximize
image quality. Once the image is captured it can
be emailed, posted to social media sites, or sent
by text.
The Discovery/Doc features a platform that
supports a smart phone or a tablet. This is
combined with a portable photographic hood that
is designed specifically for stable gel photography.
w/Fluorescent Red Filter
w/Fluorescent Green Filter
w/Neutral Density Filter
E6-2750........................ $550
E6-2850 ........................ $675
E6-2950 ........................ $450
Compatible stains
• Ethidium bromide
• Gel Red
• SYPRO Ruby
Compatible stains
• FOTO/Vision
• SYBR Safe
• SYBR Green
Compatible stains
• Methylene blue
• Coomassie blue
Compatible with:
• Most camera phones and tablets
Includes:
• Platform for camera phone and
tablet
• Choice of photographic filter
• Photographic hood
- (13.5 x 14.25 cm base)
Suggested add-ons:
• Additional photographic filter
modules
E60-2193, fluorescent red....... $275
E60-2194, fluorescent green... $400
E60-2195, neutral density ....... $175
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MolecuLabs:MolecularBiologyKitsforYourClassroom
Easy,Hands-onLearning
MolecuLabs make it easy for you to incorporate
hands-on learning into your curriculum! The
kits are complete with instructor and student
manuals and all of the components necessary
to perform the experiment. You will love the
simplicity of the required preparations and your
students will be excited about the topics and
will anxiously await their results.
HowMolecuLabsStackUpwiththeStandards.....
MolecuLab teaching kits offer hands-on activities in a variety of themes that will engage your
students. They emphasize the NGSS Disciplinary Core Ideas and the AP® Biology Big Ideas allowing
them to be included throughout your curriculum with ease!
AP®Biology Big Ideas
NGSS Disciplinary Core Ideas
MolecuLab
LS1
LS2
Molecular Evolution
LS3
LS4
1
X
X
X
2
3
4
Page
X
X
5
Do Vampires Walk Among Us?
X
X
X
X
6
Paper Sequencing
X
X
X
X
8
X
X
6
DNA Profiling Simulation:
Whooping Cranes
X
X
X
X
DNA Profiling Simulation:
A Forensic Case
X
X
7
7
Bioluminescent Bacterial
Transformation
X
X
X
X
Genomics & Microarrays
X
X
X
X
5
Paper Microarray
X
X
X
X
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E-mail:[email protected]•Web-site:www.fotodyneclassroom.com
MolecuLabClassroomKits
Tasha, the DNA source of the first
complete dog genome sequence.
MolecularEvolution:WhatCanDogsTeachUs?
MolecuLab229&230
“Man’s best friend” was domesticated from the
ancestor great wolf. This type of selective breeding
offers a unique opportunity to learn about evolution.
In the pre-lab activity, students use photos of the
gray wolf and seven different modern dog breeds in
an effort to build a phylogenetic tree based solely on
appearance of the animals.
Results from MolecuLab 229: Sample
gel from one student team showing
molecular differences between the gray
wolf and seven modern dog breeds.
Ordering Information:
At the lab bench students use molecular information.
Each team will receive simulated DNA samples from
the gray wolf and the modern breeds. Students will
perform electrophoresis of PCR products to reveal
their restriction fragment length polymorphism
(RFLP) patterns. Together the groups will determine
the number of molecular differences between the
and use this information to build a phylogenetic tree
that depicts the relatedness of the ancestor gray wolf
and the modern breeds.
MolecuLab 229
MolecuLab 230
(FOTO/Vision Fluorescent stain)
(methylene blue stain)
Full Kit
E1-1229
$125
E1-1230
$125
Replacement Biologicals
only
E1-2229
$70
E1-2230
$70
Concepts
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Evolution
Genotype and phenotype
DNA structure & sequence
Phylogenetic trees
RFLPs
Hands-on Skills
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Micropipetting
Gel analysis
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•
Electrophoresis
Photo-documentation
Logistics
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8 student teams
One or two 50-minute class periods
Full Kit Includes:
Replacement Biologicals:
Dehydrated DNA samples for gel
electrophoresis, 8 SNP sets
(MolecuLab 229 is stained with FOTO/Vision)
1X gel loading dye
RFLP Analysis poster
•
•
•
Supplies:
Agarose powder
TBE buffer powder
Methylene Blue (230 only) stain
Staining trays (230 only)
Gloves
Dog Details handout
CD containing student protocols, instructor’s
manual, and PowerPoint presentation
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Genomics&Microarrays:ExploringSmokingandLungCancer
MolecuLab420
Developed by a
biotechnology
educator, this
intermediatelevel exercise
lets your
students
connect
genotype to
phenotype by making and hybridizing
a DNA chip using genes related to
lung cancer and smoking.
Students start by conducting their
own clinical research survey and then
viewing healthy and diseased lung
tissue using web-based resources.
Ordering Information:
Full Kit
Replacement Biologicals
only
To do the microarray experiment,
they choose which human genes to
spot on a DNA chip after conducting
a BLAST search. The genes are
hybridized with target DNA from
smokers, non-smokers or former
smokers.
Concepts
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Gene expression
DNA structure
Complementary base pairing
Genotype to phenotype
Hands-on Skills
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BLAST search
Experimental design
Pipetting
Logistics
After hybridization, the DNA chips are
sent to the University of Wisconsin
Gene Expression Center for scanning.
Students will view color images of
their chips for observing and analyzing
genotypic differences. Images are
posted and can be downloaded from
FOTODYNE’s website.
MolecuLab 420
E1-4020
$190
E1-4220
$150
Includes a
BLAST
search.
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8 student teams
Two 50-minute class periods for chip spotting
and hybridization
One 50-minute class period for results &
analysis
Full Kit Includes:
Replacement Biologicals:
•
Slides & coverslips
•
Simulated DNA:
- smoker
- non-smoker
- former smoker
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Probe DNA
•
Hybridization buffer
SDS solution
SSC buffer solution
Pre-labeled,
postage paid mailer
Scanning fee
(8 slides)
Supplies:
•
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Sterile clear & amber tubes w/caps
50 ml conical tubes
CD containing student protocols, instructor
manuals, gene chip paper lab, and PowerPoint
presentation
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E-mail:[email protected]•Web-site:www.fotodyneclassroom.com
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MolecuLabClassroomKits
DNAProfilingSimulation:AWhoopingCranePaternityTest
MolecuLab207&208
MolecuLabs 207 and
208 offer an alternative
to the “crime scene”
DNA Profiling scenario.
These kits link molecular
biology to environmental
biology by illustrating
how wildlife biologists
use Short Tandem
Repeat (STR) analysis
by PCR (Polymerase
Chain Reaction) to aid in
the preservation of the
endangered Whooping
Crane.
“I must tell you that both
groups really liked the
Whooping Crane Paternity
Lab. It incorporates 3 out
of 4 of the Big Ideas in the
AP Biology curriculum and
that’s very cool… They
liked how easy it was to
do, and the results were
great. The instructions
were clear and easy to
follow, too, which makes
the kit so student-friendly.”
- Cheryl Hollinger,
Central York High School
Ordering Information:
STRs are genetic markers
used to determine identity.
Since it takes several
STRs to determine identity,
each team in the class
will receive a different
PCR amplified STR from
a set of Whooping
crane individuals to
analyze by agarose gel
electrophoresis. After
every team completes their
analysis, the class will
complete the DNA profiles
together to determine
parentage.
MolecuLab 207
MolecuLab 208
(FOTO/Vision Fluorescent stain)
(methylene blue stain)
E1-2070
$130
E1-2270
$75
E1-2080
$130
E1-2280
$75
Full Kit
Replacement Biologicals
only
Concepts
•
•
•
•
STR analysis
PCR
DNA profiling - paternity
Chromosomal inheritance
Hands-on Skills
•
•
•
•
Pipetting
Electrophoresis
Photodocumentation
Gel analysis
Logistics
•
•
8 student teams
One or two 50-minute class periods
Full Kit Includes:
Replacement Biologicals:
•
Dehydrated DNA samples for gel
electrophoresis, 8 STR family sets.
1X gel loading dye
Allelle Analysis poster
•
•
Supplies:
•
•
•
•
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•
Agarose powder
TBE buffer powder
Methylene Blue (208 only) stain
Staining trays (208 only)
Gloves
CD containing student and instructor’s manuals
DoVampiresWalkAmongUs?AGeneticTestingScenario
MolecuLab217&218
Some historians suggest
that inexplicable and
unusual symptoms
associated with diseases
may have been
confused with occult and
supernatural phenomena.
In this fun lab your
students will learn about
genetic testing and will
use Restriction Fragment
Length Polymorphisms
(RFLPs) to analyze
Results from MolecuLab 217
the DNA from a small
population of town folks for
genes related to a blood
disease called porphyria.
Concepts
Each team will receive
DNA samples from five
different patients plus three
controls and will use gel
electrophoresis to compare
band patterns. Diagnosis
for some of these patients
will require collaboration
among all teams.
Hands-on Skills
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•
•
•
•
•
•
•
•
Genetic testing
Genotype and phenotype
Patterns of inheritance
DNA sequencing
RFLPs
Micropipetting
Electrophoresis
Gel analysis
Photo-documentation
Logistics
•
•
8 student teams
One or two 50-minute class periods
Full Kit Includes:
Replacement Biologicals:
“The topic of vampires serves as an excellent hook, but once they get into it, they
are fascinated by the actual science, as most of them have never experienced gel
electrophoresis firsthand before. The kids really love this lab! :)”
- Pat Burton,
Mercy Academy, Louisville KY
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•
Agarose powder
MolecuLab 217
TBE buffer powder
Ordering Information:
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Supplies:
(methylene blue stain)
MolecuLab 218
•
Methylene Blue (218 only) stain
E1-2170
$135
E1-2370
$100
E1-2180
$135
E1-2380
$100
•
Staining trays (218 only)
•
Gloves
•
CD containing student and instructor’s manuals
and PowerPoint presentations
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1X gel loading dye
(FOTO/Vision Fluorescent stain)
Replacement Biologicals
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electrophoresis, 8 patient sets.
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Full Kit
on
Dehydrated DNA samples for gel
Phone:1-800-DNA-FOTO(800-362-3686)
E-mail:[email protected]•Web-site:www.fotodyneclassroom.com
MolecuLabClassroomKits
DNAProfilingSimulation:AForensicCase
MolecuLab105&106
A crime has been
committed in your
science lab. You can
solve this crime by using
“DNA Fingerprinting”.
You organize your
students into cooperative
teams consisting of a
research scientist, a
lab technician, a quality
control officer, and a lab
manager. Each team
must then develop a
plan of action, using
concept maps and flow
Ordering Information:
Full Kit
Replacement Biologicals
only
MolecuLab 105
charts, to proceed with
the investigation.
Concepts
Each team of students
will receive four
tubes containing
DNA predigested with
different restriction
enzymes. After agarose
gel electrophoresis,
view the separated DNA
fragments on for an
incriminating analysis.
Hands-on Skills
•
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•
•
MolecuLab 106
(methylene blue stain)
E1-2005
$115
E1-2205
$75
E1-2006
$115
E1-2206
$75
Pipetting
Electrophoresis
Photodocumentation
Gel analysis
Logistics
•
•
•
8 student teams
One 50-minute class period for crime scene
analysis and plan development
One or two 50-minute class periods for lab
exercise
Full Kit Includes:
Replacement Biologicals:
•
(FOTO/Vision Fluorescent stain)
DNA profiling - identity
Cooperation of experts
Concept mapping
•
Dehydrated DNA samples for gel
electrophoresis, 8-tubes of each: Victim,
Evidence, Suspect 1, Suspect 2
1X gel loading dye
•
•
•
•
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•
Agarose powder
TBE buffer powder
Methylene Blue (106 only) stain
Staining trays (106 only)
Gloves
CD containing student and instructor’s manual
Supplies:
BioluminescentBacterialTransformation
MolecuLab115
In this exercise, your students will transform
recombinant plasmids containing the lux operon for
bacterial bioluminescence into E. coli.
Concepts
•
Bacterial bioluminescence
Hands-on Skills
After transformation, students will see
bioluminescent colonies on an agar plate.
•
Bacterial transofrmation
•
Colony isolation
Logistics
This exercise uses techniques similar to those in AP
Investigation #8.
About Bacterial Bioluminescence
Many organisms have the ability to emit biologically
produced light. Perhaps some of the most bizarre and
fascinating of these are marine fish and squid, which have a
variety of unique light organs. However, most luminescent
marine animals do not produce bioluminescence
themselves, but harbor bioluminescent bacteria in
specialized light organs.
Unraveling DNA: Molecular Biology for the Laboratory
Winfrey, Rott, Wortman _ Prentice Hall
Full Kit
Replacement Biologicals
only
8 student teams
•
Three 50-minute class periods
Kit Includes:
Replacement Biologicals:
•
E. coli strain DH5∝
•
Recombinant plasmid solution
•
LB/Amp and LB agars with petri dishes
•
Instructor control strains
Supplies:
•
Ordering Information:
•
MolecuLab 115
•
Sterile CaCl2
Sterile inoculating loops
E1-1115
$175
E1-2215
$70
•
Sterile LB broth
•
Cell spreaders
•
Sterile culture tubes
•
CD containing student and instructor’s manuals
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MolecuLabClassroomKits-PaperLabs
Stayattheforefrontofteachingbiologyandbiotechnology.Thesepaperkitsovercome
theobstaclesofshortclasstimes,expensiveequipmentandreagents!
PaperMicroarray:LungCancer&Smoking
MolecuLab42
PaperSequencing:AClassroomExercise
MolecuLab45
Kit Includes:
Kit Includes:
•
•
28 precut paper mRNA
1 self-adhesive poster-size
gel with molecular weight size
transcripts
•
ladder
1 self-adhesive poster-size chip
•
containing Probes to hybridize
student and instructor manuals
to the Target
•
32 stickers to affix to the Target
prior to hybridization
Bring the excitement of
microarray technology
to your students with
this group paper activity.
MolecuLab 42 explores
the gene expression
differences between
smokers and nonsmokers. Your students
will be able to predict and
analyze the relationship
between genotype and
phenotype.
•
CD containing student reading,
student and instructor manuals
Concepts
•
Gene expression
•
DNA structure
•
Complementary base pairing
•
Genotype to phenotype
Logistics
•
8 student teams
•
One 50-minute class period
•
No equipment needed!
CD containing student reading,
This paper experiment
allows you to integrate
a technologically
advanced molecular
biology technique in
your classroom. Your
students are “scientists”
and receive DNA from
an unknown genome. All
students participate and
elucidate the sequence.
With this kit, the power
of genomic sequencing
comes to life!
•
32 precut paper DNA templates
•
32 precut paper DNA primers
•
Precut paper
“dNTPs and ddNTPs”
– see website for details
Concepts
•
DNA structure
•
Complementary base pairing
Logistics
•
8 student teams
•
One 50-minute class period
•
No equipment needed!
Ordering Information:
MolecuLab 42
Ordering Information:
MolecuLab 45
E1-0420
$25
E1-0450
$35
FOTO/VisionDNAStain-ASafeAlternativetoEthidiumBromide
Ordering information:
E1-1843, 1 ml................................ $35
(enough for 500 lanes)
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Features:
• Convenient - supplied in 6X loading buffer.
Dilute 1 part FOTO/Vision with 5 parts DNA
sample and mix.
• Time-saving - Mix with DNA sample, run
on gel and immediately visualize bands on
a UV transilluminator.
• Performance - extremely low background
fluorescence.
• Safe - Results from Ames test performed
by independent testing lab demonstrates
that FOTO/Vision is non-mutagenic.
• Many applications - cloning including
ligation and transformation procedures,
PCR, Southern blotting, teaching labs, high
throughput screening.
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FOTO/Vision DNA Stain
FOTO/Vision is a nonmutagenic fluorescent
alternative to ethidium
bromide that provides
immediate band
visualization. This DNA
stain doubles as a loading
buffer and tracking dye.
There is no hazardous
shipping, storage, or
waste.
Phone:1-800-DNA-FOTO(800-362-3686)
E-mail:[email protected]•Web-site:www.fotodyneclassroom.com