Crowsnest River Drainage Sport Fish Population Assessment, 2010
Transcription
Crowsnest River Drainage Sport Fish Population Assessment, 2010
Crowsnest River Drainage Sport Fish Population Assessment, 2010 The Alberta Conservation Association is a Delegated Administrative Organization under Alberta’s Wildlife Act. 25% Post Consumer Fibre When separated, both the binding and paper in this document are recyclable Crowsnest River Drainage Sport Fish Population Assessment, 2010 Jason Blackburn Alberta Conservation Association #1609, 3 Avenue South Lethbridge, Alberta, Canada T1J 0L1 Report Editors PETER AKU Alberta Conservation Association #101, 9 Chippewa Rd Sherwood Park AB T8A 6J7 GLENDA SAMUELSON R.R. #2 Craven SK S0G 0W0 Conservation Report Series Type Technical ISBN printed: 978-0-7785-9390-4 ISBN online: 978-0-7785-9391-1 Publication No.: T/243 Disclaimer: This document is an independent report prepared by the Alberta Conservation Association. The authors are solely responsible for the interpretations of data and statements made within this report. Reproduction and Availability: This report and its contents may be reproduced in whole, or in part, provided that this title page is included with such reproduction and/or appropriate acknowledgements are provided to the authors and sponsors of this project. Suggested Citation: Blackburn, J. 2011. Crowsnest River drainage sport fish population assessment, 2010. Technical Report, T-2011-001, produced by the Alberta Conservation Association, Lethbridge, Alberta, Canada. 27 pp + App. Cover photo credit: David Fairless Digital copies of conservation reports can be obtained from: Alberta Conservation Association #101, 9 Chippewa Rd Sherwood Park AB T8A 6J7 Toll Free: 1-877-969-9091 Tel: (780) 410-1998 Fax: (780) 464-0990 Email: [email protected] Website: www.ab-conservation.com i EXECUTIVE SUMMARY The Crowsnest River is one of the most popular trout fisheries in Alberta. However, increased angling pressure, habitat degradation from recreational and industrial activities, and the invasion of less popular introduced species threaten the fishery. This study monitors populations of rainbow trout and native mountain whitefish, the two primary species in the sport fishery, using electrofishing and mark-recapture techniques. Of 3,979 salmonid fish captured in the Crowsnest River, 65% were rainbow trout and 30% were mountain whitefish. The proportion of the mountain whitefish catch that was legal-harvest-sized, quality-sized, and above the slot size was 44%, 25% and 6%, respectively; greater than the proportion of the rainbow trout catch in these size categories at 11%, 7%, and 2%, respectively. The total tributary catch was 1,085 fish, and was dominated by Westslope cutthroat trout, rainbow trout, and their hybrids (Oncorhynchus species) at 84% combined, and respectively at 42%, 27%, and 14% of the catch. Estimated abundance of rainbow trout in the main-stem of the Crowsnest River was 80,131 fish of which 8,501 were legal-harvest-sized, 5,290 were quality-sized, and 1,445 were above the slot size. Estimated mountain whitefish abundance was 16,517 fish, with 7,340 legal-harvest-sized, 3,816 quality-sized, and 1,743 above the slot size. While the total estimated main-stem abundance was nearly five times greater for rainbow trout, the proportion of legal-harvest-sized fish, quality-sized fish, and fish above the slot size was greater for mountain whitefish. Total estimated tributary populations of Oncorhynchus trout species was 60,637 individuals, of which 34% resided in Blairmore Creek. Both the highest main-stem fish densities and the highest incidence of hooking damage occured between the Highway 507 and the East Hillcrest bridge crossings. Incidence of hooking damage for all rainbow trout, those of legal-harvest-size, quality-size, and above the slot size averaged 3%, 16%, 9%, and 9%, respectively; whereas mountain whitefish hooking damage averaged 3%, 3%, 3%, and 4%, respectively. Invasive brook trout and brown trout were most abundant in the upper reaches of the main-stem river, upstream of the town of Frank, and in the tributaries of Drum Creek, ii Gold Creek, and Allison Creek. Bull trout were captured only in the main-stem river, downstream of Lundbreck Falls. Westslope cutthroat trout populations were most intact in the tributaries of Island, Giardi, Star, upper Blairmore, upper Gold and upper Rock Creeks. Upstream of Lundbreck Falls, rainbow trout and mountain whitefish were the dominant species, whereas downstream of Lundbreck Falls sucker species were dominant. Small sized fish were most strongly represented in the main-stem population of rainbow trout, whereas the mountain whitefish population structure showed no trend in capture frequency by size, with a relatively homogenous representation of fish across the full range of sizes. Key words: Crowsnest River, rainbow trout, mountain whitefish, cutthroat trout, abundance. iii ACKNOWLEDGEMENTS Funding for this study was provided by Alberta Conservation Association (ACA) and Devon Canada Corporation. Many thanks to the ACA fisheries crew: Brad Hurkett, Andrew Clough and Clint Goodman. Thanks to additional ACA staff who helped in data collection including Peter Aku, Trevor Council, Mike Jokinen, Tyler Johns, Jason Letham, Marcel Macullo, Mike Marquardson, Matthew Szumilak and Mike Uchikura. Many thanks to Kevin Fitzsimmons for generating abundance distribution figures, Mike Rodtka and Paul Hvenegaard for project feedback, and Mandy Couve for report review. This project would not have been possible without the guidance of Trevor Council. Thanks to our government partners at Alberta Sustainable Resource Development, including Matthew Coombs for assistance with field sampling and Daryl Wig for consultation about the study area and provision of additional field equipment. I extend my thanks to Bryan Sundberg (Alberta Parks, Tourism and Recreation) for accommodations at Beauvais Lake. Special thanks to the Oldman Chapter of Trout Unlimited Canada (TUC) for granting access to TUC property, and to the sampling volunteers, Geordie Paton and Kevin Watson. iv v TABLE OF CONTENTS EXECUTIVE SUMMARY .......................................................................................................... ii ACKNOWLEDGEMENTS ....................................................................................................... iv TABLE OF CONTENTS ........................................................................................................... vi LIST OF FIGURES .................................................................................................................... vii LIST OF TABLES ..................................................................................................................... viii LIST OF APPENDICES ............................................................................................................ ix 1.0 INTRODUCTION .......................................................................................................... 1 2.0 STUDY AREA ................................................................................................................. 2 3.0 MATERIALS AND METHODS ................................................................................... 4 3.1 Sampling intensity ..................................................................................................... 4 3.2 Sample reach length and site placement ................................................................ 5 3.3 Data collection ............................................................................................................ 6 3.4 Population density and abundance ........................................................................ 7 3.5 Capture probability (q).............................................................................................. 8 3.6 Density and abundance calculations ...................................................................... 8 3.7 Abundance distribution ............................................................................................ 9 4.0 RESULTS ......................................................................................................................... 9 4.1 Fish capture summary .............................................................................................. 9 4.2 Population abundance and density ...................................................................... 10 4.3 Trends in main-stem river species distributions ................................................. 13 4.4 Distribution of main-stem river fish abundance ................................................. 15 4.5 Tributary species distribution ................................................................................ 17 4.6 Population structure and size distribution .......................................................... 19 4.7 Incidence of main-stem river hooking damage ................................................... 23 4.8 Summary ................................................................................................................... 24 5.0 LITERATURE CITED .................................................................................................. 25 6.0 APPENDICES ............................................................................................................... 28 vi LIST OF FIGURES Figure 1. Crowsnest River drainage study area and sampling locations in 2010. ......... 3 Figure 2. Trends in main-stem species distributions from lower reaches at the mouth of Todd Creek to upper reaches at the outlet of Crowsnest Lake, based on tote-barge capture data. ....................................................................................... 13 Figure 3. Comparison of species composition of electrofishing catch from the Crowsnest River in 2010, a) upstream of Lundbreck Falls, and b) downstream of Lundbreck falls.......................................................................... 14 Figure 4. Estimated rainbow trout and mountain whitefish abundance per 250 m in the Crowsnest River as measured from the mouth of Todd Creek............... 16 Figure 5. Species composition of electrofishing catch by tributary watershed in the Crowsnest River drainage in 2010...................................................................... 18 Figure 6. Length-frequency distributions of fish captured in the Crowsnest River in 2010. ........................................................................................................................ 20 Figure 7. Length-frequency distributions of fish captured in the Crowsnest River tributaries in 2010. ................................................................................................ 22 vii LIST OF TABLES Table 1. Number of fish, by size range, captured during totebarge and backpack electrofishing in the Crowsnest River and its tributaries in 2010. ................. 10 Table 2. Estimated abundance and density, by size range, of rainbow trout and mountain whitefish in the Crowsnest River in 2010 ....................................... 11 Table 3. Estimated abundance of sport fish in major tributary watersheds of the Crowsnest River drainage in 2010...................................................................... 12 Table 4. Size distribution of fish electrofished in the Crowsnest River in 2010. ........ 19 Table 5. Size distribution of fish electrofished in tributaries to the Crowsnest River drainage in 2010. ................................................................................................... 21 Table 6. Proportion (%) of the first pass catches of rainbow trout and mountain whitefish with evidence of hooking damage.................................................... 23 viii LIST OF APPENDICES Appendix 1. Capture summary from Crowsnest River preliminary tote-barge eletrofishing in 2009 ........................................................................................ 28 Appendix 2. Summary of cooperative fisheries inventory program data used in the Crowsnest River tributaries study design ................................................... 29 Appendix 3. Crowsnest River sample site locations in 2010 ........................................... 32 Appendix 4. Crowsnest River tributary sample site locations in 2010 ........................... 33 Appendix 5. Crowsnest River measurement data in 2010 .............................................. 35 Appendix 6. Crowsnest tributary stream measurement data in 2010............................ 36 Appendix 7. Fish catch at 2010 sampling sites used to estimate abundance by size class ................................................................................................................... 38 Appendix 8. Sport fish catch at 2010 tributary sample sites used to determine corrected abundance per site ......................................................................... 39 Appendix 9. Summary of Peterson capture-mark-recapture (CMR) capture probabilities (q) used to correct 2010 single-pass backpack electrofishing catch. ................................................................................................................. 41 Appendix 10. Summary of Jolly-Seber capture-mark-recapture probabilities used to correct 2010 main-stem Crowsnest River single-pass tote-barge electrofishing catches...................................................................................... 42 Appendix 11. Electrofishing catch at 2010 sampling sites used to describe species distributions in the Crowsnest River ........................................................... 43 Appendix 12. Proportion (%) of the 2010 first pass electrofishing catch of rainbow trout and mountain whitefish, by sampling site and size range (mm), with evidence of hooking damage......................................................................... 44 ix 1.0 INTRODUCTION The Crowsnest River has a long standing reputation as a quality fishery for large rainbow trout (Oncorhynchus mykiss) and mountain whitefish (Prosopium williamsoni), drawing anglers to the Crowsnest Pass from considerable distances. Numerous outfitters and guides make a living from the sport fishery, as well as, local fly shops and businesses that benefit from visiting anglers. The Crowsnest River is considered one of the most popular trout fisheries in Alberta. An angler survey conducted in 2001, indicated that 12,000 anglers fished a total of 32,000 hours on the portion of the Crowsnest River between Crowsnest Lake and the Oldman Reservoir, during the summer of 2001 (June–September) targeting mainly rainbow trout and mountain whitefish (Genereux and Bryski 2002). The sport fishery in the Crowsnest River drainage has undergone considerable change as a result of landscape disturbance and past fisheries management decisions. The historical sport fish assemblage in the Crowsnest River main-stem included mountain whitefish, bull trout (Salvelinus confluentus), and Westslope cutthroat trout (Oncorhynchus clarkii lewisi). Through several plantings in the 1930s and 1940s, rainbow trout became established, displacing native cutthroat trout and restricting them to select tributaries (Alberta Sustainable Resource Development (ASRD) and Alberta Conservation Association (ACA) 2006; Taylor and Gow 2007). Bull trout, which once occurred in tributaries above and below Lundbreck Falls (Fitch 1997), have been restricted to the main-stem river below the falls (ASRD and ACA 2009) as a result of over harvest and habitat loss. Currently at risk are the remnant populations of cutthroat trout, as well as the highly popular mountain whitefish and exotic rainbow trout fishery in the main-stem river. Continued habitat degradation from recreational, industrial and municipal development activities, as well as competition from less desirable exotic species threaten the sport fishery. Species introduced into the drainage since the 1960s include lake trout (Salvelinus namaycush), brown trout (Salmo trutta) and brook trout (Salvelinus fontinalis). In an effort to maintain the Crowsnest River as a quality sport fishery monitoring of sport fish populations is a high priority. Thus, the main objectives of this study were to: 1 • Estimate the abundance and density of sport fish populations in the Crowsnest River. • Estimate the abundance and density of native trout species in the Crowsnest River drainage tributaries. • Measure and document the extent of hooking damage to rainbow trout and mountain whitefish from the Crowsnest River where angling pressure is considered high. 2.0 STUDY AREA The Crowsnest River originates from Crowsnest Lake in the Rocky Mountains of southern Alberta, near the continental divide on the Alberta/British Columbia border. The Crowsnest River flows eastward for approximately 57 km before converging in the Oldman Reservoir with the Castle River to the south, and the Oldman River to the north. The Crowsnest River flows through several local municipalities including Coleman, Blaimore, Frank, Belleve and the hamlet of Hillcrest, which together comprise the Municipality of Crowsnest Pass. Our study area included the entire Crowsnest River west of its confluence with Todd Creek, and all known fish-bearing tributaries west of Highway 22 (Figure 1). Major tributary watersheds originating to the west of the mountain front include Star and Allison Creeks near Crowsnest Lake, McGillvray Creek, York Creek, Lyons Creek, Blairmore Creek, and Gold Creek. To the east of the mountain front, major tributary watersheds include Todd Creek, Cow Creek, Connelly Creek, and Rock Creek. While primarily a volunteer catch-and-release fishery (Genereux and Bryski 2002), harvest of fish is permitted in the Crowsnest River drainage under current sport fishing regulations. A daily possession limit of two trout is permitted on all sections of the Crowsnest River between 16 June and 31 October (except for bull trout which is catchand-release only in Alberta), and for all its tributaries between 16 June and 16 August (except Gold Creek see below). Size restrictions apply only for rainbow trout and cutthroat trout. In the upper reaches of the Crowsnest River, harvested fish must be greater than 30 cm, and the use of some baits are permitted after 15 August. In the middle and lower reaches of the river no bait is permitted and there is a slot size 2 Figure 1. Crowsnest River drainage study area and sampling locations in 2010. 3 restriction where only one harvested fish may be greater than 45 cm and the remainder must be less than 30 cm. A daily possession limit of five mountain whitefish greater than 30 cm is permitted throughout the Crowsnest River for the duration of the legal angling season, however only until 31 August in all its tributaries (ASRD 2010). The exception is Gold Creek, where only brook trout may be harvested and the possession limit is two fish per day for the duration of the angling season. 3.0 MATERIALS AND METHODS Study design and sampling effort was determined through power analysis methods using archived data from the Cooperative Fisheries Inventory Program (CFIP) (Fisher 2000; Jokinen 2002; Faulter 2003), and the Alberta Government Fisheries and Wildlife Management Information System (FWMIS) database. When delineating the study area, I excluded the river downstream of Todd Creek because fluctuating levels of the Oldman Reservoir continually flood this reach. I used highway 22 as a geographic and topographic boundary between mountain/foothill and prairie stream types. Based on FWMIS capture data, fish community types were typically dominated by salmonid sport species west of the highway and minnow/sucker non-sport species to the east of the highway. I excluded first-order and second-order streams because they are typically ephemeral or dry and fishless within the study area (Fisher 2000; Jokinen 2002; Faulter 2003), as well as stream reaches known to be fishless from past repeated inventory sampling. Forty-five tributary reaches and 10 main-stem river reaches were visited during a reconnaissance survey to verify stream flow and collect stream width data to help delineate study area boundaries. 3.1 Sampling intensity I queried archival FWMIS data and summarized species distributions and relative abundances to identify data gaps and prioritize preliminary sampling. The Crowsnest River main-stem was data deficient so preliminary electrofishing was conducted at five reaches along the Crowsnest River in 2009 (Appendix 1), two located in the upper reaches, two in the middle reaches, and one below Lundbreck Falls. Using preliminary electrofishing abundance results, I conducted power analysis to determine sampling 4 intensity for the summer of 2010. I considered the minimum functional degree of precision for abundance estimates to be within 50% of the population mean, or a “sample survey” level of precision (Schwartz 2005) while endeavoring to attain as close to “management precision” levels (within 25% of the population mean) as possible. Use of power analysis on a preliminary main-stem Oncorhynchus abundance mean (±SD) of 21,412 ± 10,023, indicated 14 sample reaches were required to achieve a management level of precision. Considering main-stem abundance data was limited, I also estimated the maximum number of sample reaches possible while maintaining sampling independence between reaches (one sample reach length between sites). Based on 34 potential sample reaches on the main-stem (reach lengths estimated using reconnaissance width measurements), in conjunction with anticipated logistic and seasonal time constraints, I decided on 22 main-stem river sample reaches comprising approximately 30% of the total river length. From 67 inventory sites (Appendix 2), I calculated a tributary Oncorhynchus population mean (±SD) of 37,905 ± 45,314. Using power analysis, approximately 88 sample reaches were required to achieve a management level of precision. Consequently, I decided on a survey level of precision, requiring a more feasible 42 sample reaches on the tributaries with a predicted relative standard error (RSE) of 37%. 3.2 Sample reach length and site placement In the absence of quantitative riffle and pool data, which directly relate to species abundance patterns (Taylor 2000), I used stream size as a surrogate measure of fish habitat. On the Crowsnest River, sample reach lengths were based on 40 times the mean wetted width (minimum of 35 times mean wetted width; Lyons 1992), to a maximum of 800 m total length (in order to maintain sample reach independence). Sample reaches were spaced systematically along the length of the main-stem river (Appendix 3) using GIS software. Sample reaches that landed on lakes or hazards (e.g. Lundbreck Falls), were repositioned elsewhere on the main-stem, while maintaining sample reach independence. On the tributaries, sample sites were determined from a systematically distributed pool 5 of 42 points. Non-response sites (e.g. dry channels and beaver ponds) and redundant sites (e.g. sites clustered in dense headwater tributaries) were repositioned. positioning of sites ensured: Final minimization of gaps between sites, preferential representation of wider, lower reaches, and representation below, between and above partial fish passage barriers (Appendix 4 and Figure 1). Consideration of barriers was intended to account for the incremental impact barriers have on the presence of pure Westslope cutthroat trout (Robinson 2007), as well as the effects of offspring reduction caused by hybridization (Muhlfeld et al 2009), and the subsequent effect on site-level cutthroat trout densities. Tributary sample reach lengths were 40 times the mean wetted width, with a minimum length of 150 m (Reynolds et al. 2003). On inventory and reconnaissance measurements, all tributary sample reach lengths were estimated at approximately 300 m or less. Consequently, all tributary sample reach lengths were standardized to 300 m. 3.3 Data collection Fish were collected using electrofishing gear. Smith-Root backpack electrofishers, types 15 and 12B, were used for tributary sample reaches and a Smith-Root LR-6 tote-barge electrofisher was used on the main-stem river sample reaches. We backpack electrofished in crews of two in an upstream progression, with one dipnetter and one electrofisher operator. We tote-barge electrofished using a four-member crew in a downstream progression, with one tote-barge operator, one anode pole operator and two dipnetters. All captured fish were retained in live-wells, measured, and returned to the stream a short distance in the opposite direction of sampling. Biological data collected included species, fork length (FL, mm), total length (TL, mm), weight (g), and evidence of hooking damage, such as maxillary tears and scarring to the premaxilla and mouth corners. We also collected adipose and caudal fin clips from suspected pure and hybrid cutthroat trout in support of the provincial Westslope Cutthroat Trout Recovery Plan (ASRD 2009). Tissue samples were stored in labeled 2-mL Cryo-vials in 100% anhydrous-ethyl-alcohol for DNA analysis. 6 Electrofishing transects were spaced at 50 m intervals for tributary reaches and 100 m intervals for main-stem reaches. Habitat data collected along transects included; wetted width, rooted width, thalweg depth and the percent of the transect comprised by pools, riffles and runs (Appendix 5 and 6). Electrofishing effort in seconds(s) was also recorded. Cutthroat trout, rainbow trout, and their hybrids were distinguished using external morphological characteristics as per Robinson (2007). 3.4 Population density and abundance I estimated total abundance and density (fish/m and fish/km) for the length of the Crowsnest River for mountain whitefish and rainbow trout by size range. Estimates with 90% confidence intervals (CI) were calculated using single pass capture data (Appendix 7). Size ranges included: 1) all fish susceptible to our electrofishing gear (>74 mm TL for rainbow trout and >77 mm TL for mountain whitefish; ACA file data), 2) legal-harvest-sized fish (>300 mm TL; ARSD 2010), 3) quality-sized fish (i.e. rainbow trout >400 mm TL; Gabelhouse 1984), and 4) fish above the slot size restriction (>450 mm TL, possession limit of one). Main-stem river catches of cutthroat trout, rainbow trout, and their hybrids were combined for abundance calculations because of the limited number of cutthroat trout and hybrid captures and their presumed similar capture probabilities. Other sport species with unknown capture probabilities and catch rates too low to generate reliable estimates were excluded from main-stem abundance calculations. I estimated tributary abundance and density for prominent watersheds containing native trout species, as well as all tributaries combined, using single pass capture data (Appendix 8). All abundance estimate calculations for both the main-stem Crowsnest River, and its tributaries were performed using stream lengths derived from the Alberta base data single line hydrology network. 7 3.5 Capture probability (q) To determine capture probability (q), I performed capture-mark-recapture (CMR) surveys and calculated site abundances using the Ecological Methodology program from Krebs (1999). Three closed-model abundance estimates were carried out on tributaries with a single mark run and a single recapture run, conducted on consecutive days. Tributary sections were isolated using blocking nets for the duration of the CMR surveys and backpack electrofishing was used to collect the fish. Peterson estimates were calculated for the tributary abundance sites with 95% confidence intervals. Capture probability was the proportion of the abundance estimate represented by the first pass catch (i.e. first pass ÷ Peterson estimate). I supplemented Crowsnest River tributary capture probabilities with those calculated for other backpack electrofished CMR on like-sized streams (orders 3 – 5) in the upper Oldman and Castle River drainages (Blackburn 2010), in order to correct single-pass catch rates (Appendix 9). On the Crowsnest River we conducted open model (Jolly-Seber) estimates, performing three passes of tote-barge electrofishing per abundance estimate. Five main-stem river CMR ranged from 4 to 21 days between the first and last sample periods (2 estimates spanned 4 days, 2 spanned 10 days and 1 spanned 21 days). Jolly-Seber abundance estimates were calculated for the Crowsnest River and capture probability was represented as the first alpha value generated (i.e. the proportion marked) between pass 1 and pass 2 using Ecological Methodology (Krebs 1999) (Appendix 10). Five capture probabilities were used to generate a beta distribution to calculate rainbow trout abundance. Three were used to generate mountain whitefish capture probability distributions, because of the assumption violation of equal catchability, caused by observed migratory fish behavior between sampling periods. 3.6 Density and abundance calculations I generated separate randomly sequenced beta-distributions of 10,000 capture probabilities for backpack and tote-barge CMR capture probabilities, in the program R© (R Development Core Team 2009). To determine site density by stream length, I bootstrapped (10,000 runs) site capture density totals (fish captured per meter per site; C/m) by species and by size range, to generate distributions of randomly-sequenced 8 capture density means. I divided the capture density distribution by the capture probability distribution to generate a distribution of estimated fish density per site (total fish per meter per site; N/m). The mean density (N/m) distribution was sorted and the 500th and 9,500th means were selected as 90% confidence intervals. Total fish abundance was calculated by multiplying the density distribution by total stream length, and selecting 90% confidence intervals. 3.7 Abundance distribution Using the R© software program (R Development Core Team 2009), I predicted the spatial distribution of abundance of rainbow trout and mountain whitefish along the Crowsnest River, in consecutive 250 m increments, using a generalized additive model as per Fitzsimmons and Blackburn (2009). 4.0 RESULTS 4.1 Fish capture summary At 22 main-stem sample reaches, covering approximately 30% (16 km) of the length of the Crowsnest River, we captured a total of 3,979 salmonid sport fish (Table 1). The catch was dominated by rainbow trout (65%) and mountain whitefish (30%) (n = 2,645 and 1,201, respectively). Six trout species accounted for the remaining 5%, with native bull trout and cutthroat trout each representing less than 1% of the catch. The catch of legal-harvest-sized, quality-sized, and fish above the slot size was dominated by mountain whitefish at 63%, 62%, and 57%, respectively, followed by rainbow trout at 33%, 37%, and 41% of the catch, respectively. The proportion of the mountain whitefish catch that was legal-harvest-sized, quality-sized, and above the slot size was 44%, 25% and 6%, respectively; greater in all size categories than the proportion of the rainbow trout catch in these categories at 11%, 7%, and 2%, respectively. In the tributaries, the total catch was 1,085, and was dominated by Westslope cutthroat trout, rainbow trout, and their hybrids (Oncorhynchus species) at 84% combined, and respectively 42%, 27%, and 14% of the catch (Table 1). The remainder of the tributary catch was brook trout (15%), and mountain whitefish (2%). Legal-harvest-sized fish captured in the tributaries were less than 1% of the total catch. 9 Table 1. Number of fish, by size range, captured during totebarge and backpack electrofishing in the Crowsnest River and its tributaries in 2010. Main-stem river Speciesa RNTR MNWH BLTR CTTR CRTR BNTR BKTR LKTR TOTAL All fish 2,645 1,201 14 6 31 62 17 3 3,979 > 300 mm (TL) 279 528 7 3 7 6 1 2 833 > 400 mm (TL) 185 304 2 0 0 3 0 0 494 Tributaries > 450 mm (TL) 51 70 1 0 0 1 0 0 123 All fish 291 16 0 460 155 0 163 0 1,085 > 300 mm (TL) 0 2 0 6 1 0 0 0 9 RNTR = rainbow trout, MNWH = mountain whitefish, BLTR = bull trout, CTTR = cutthroat trout, CRTR = cutthroat trout x rainbow trout hybrid, BNTR = brown trout BKTR = brook trout, LKTR = lake trout a 4.2 Population abundance and density Estimated main-stem river rainbow trout abundance was 80,131, nearly five-times greater than the mountain whitefish population at 16,517 (Table 2). Similarly, abundance of legal-harvest-size and quality-sized was higher for rainbow trout (8,510 and 5,290, respectively) than for mountain whitefish (7,340 and 3,816, respectively). Conversely, abundance of mountain whitefish above the slot size (1,743) was greater than that of rainbow trout (1,445). 10 Table 2. Estimated abundance and density, by size range, of rainbow trout and mountain whitefish in the Crowsnest River in 2010. CI = confidence intervals from bootstrapped distributions, RSE = relative standard error. Speciesa RNTR MNWH a Size class (TL, mm) >74 >300 >400 >450 >77 >300 >400 >450 Total abundance (90%CI) Fish/km (90%CI) 80,131 8,501 5,290 1,445 (42,371-141,127) (4,377-15,217) (2,530-9,725) (642-2,707) 147 16 10 3 (78-260) (8-28) (5-18) (1-5) RSE (%) 41 43 45 48 16,517 7,340 3,816 1,743 (7,658-33,023) (3,307-14,616) (1,441-8,156) (513-3,648) 30 13 7 3 (14-60) (6-27) (3-15) (1-7) 56 57 64 57 RNTR = rainbow trout, MNWH = mountain whitefish Blairmore Creek had the highest and most dense tributary population of Oncorhynchus fish comprising 34% of the total tributary abundance at 19,839 (90% CI = 10,966 – 35,713), and a density of 736 fish/km (Table 3). Tributaries with the greatest native trout (cutthroat) abundances were Blairmore Creek at 65% of the Oncorhynchus abundance (12,895 of 19,839 fish) and Gold Creek at 85% of the Oncorhynchus abundance (5,422 of 6,379 fish). 11 Table 3. Estimated abundance of sport fish in major tributary watersheds of the Crowsnest River drainage in 2010. Tributary watershed Total fish abundance (90% CI) Total density (90% CI) (fish/km) Species proportion %CTTRa %CRTRb %RNTRc Blairmore 19,839 (10,966-35,713) 736 (407-1,325) 65 23 12 Gold 6,379 (1,739-13,195) 173 (47-357) 85 12 3 Rock 3,776 (646-8,052) 152 (26-323) 58 7 35 Todd 6,155 (2,301-12,255) 123 (46-245) 54 19 27 62 (7-138) 26 7 67 Allison 1,381 (159-3,080) Connelly 1,340 (335-2,837) 56 (14-119) 25 54 21 All Tributaries 60,637 (29,595-112,490) 197 (96-366) 50 18 32 aCTTR = cutthroat trout, bCRTR = cutthroat trout x rainbow trout hybrid, cRNTR=rainbow trout 12 4.3 Trends in main-stem river species distributions We captured rainbow trout and mountain whitefish throughout the main-stem of the Crowsnest River. Brook trout were captured only in the upper reaches of the river, upstream of the town of Frank (Figure 2). We captured brown trout primarily in the upper reaches, as well as in small numbers in the middle and lower reaches (Appendix 11). Cutthroat trout and their hybrids were captured sporadically throughout the main-stem river, and lake trout were captured at only two locations, in the upper and middle reaches. Bull trout and burbot were captured only in lower reaches, downstream of Lundbreck falls. We observed a sharp change in catch composition at Lundbreck Falls, from one dominated by rainbow trout and mountain whitefish upstream of the falls, to one dominated by sucker species downstream of the falls (Figure 3). Figure 2. Trends in main-stem species distributions from lower reaches at the mouth of Todd Creek to upper reaches at the outlet of Crowsnest Lake, based on tote-barge capture data. 13 Figure 3. Comparison of species composition of electrofishing catch from the Crowsnest River in 2010, a) upstream of Lundbreck Falls, and b) downstream of Lundbreck falls. RNTR = rainbow trout, MNWH = mountain whitefish, BNTR = brown trout, LNSC = longnose sucker, CRTR = cutthroat x rainbow trout hybrid, BKTR = brook trout, CTTR = cutthroat trout, LKTR = lake trout, WHSC = white sucker, BURB = burbot. 14 4.4 Distribution of main-stem river fish abundance The combined estimate of mountain whitefish (Figure 4a) contrasts sharply with that of rainbow trout, highlighting the relative scarcity of mountain whitefish less than 300 mm in the catch. The highest abundance of combined rainbow trout sizes occurred around kilometer 25, upstream of East Hillcrest Bridge, whereas the highest abundance of all combined mountain whitefish sizes occurred near kilometer 35, near Frank. Spatial distribution of abundance for all other size categories of rainbow trout and mountain whitefish follow similar trends reaching maximum abundances at similar points along the Crowsnest River (Figure 4). Rainbow trout density for all combined size classes, as well as fish >300 mm TL, was highest at kilometer 24, near East Hillcrest Bridge; mountain whitefish densities for fish >300 mm TL, and fish >400 mm TL, peaked near kilometer 23, also near East Hillcrest Bridge. The density of rainbow trout >400 mm TL and >450 mm TL was highest at kilometers 19 and 18, respectively, near the Highway 507 crossing. Similarly, the density for mountain whitefish >450 mm TL was highest at kilometer 20, also near the Highway 507 crossing (Figure 4). 15 Rainbow trout 6000 a 5000 Mountain whitefish B A 4000 3000 2000 1000 0 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 10 15 20 25 30 35 10 15 20 25 30 35 10 15 20 25 30 35 800 bC 700 D Abundance per 250 m 600 500 400 300 200 100 0 0 5 10 15 20 25 30 35 0 5 800 700 c F E 600 500 400 300 200 100 0 0 5 10 15 20 25 30 35 0 5 800 dG 700 H 600 500 400 300 200 100 0 0 5 10 15 20 25 30 35 0 5 Distance upstream (km) Figure 4. Estimated rainbow trout and mountain whitefish abundance per 250 m in the Crowsnest River as measured from the mouth of Todd Creek for; a) all fish, b) fish >300 mm TL, c) fish >400 mm TL, d) fish >450 mm TL. Abundance estimated from single-pass electrofishing capture data at 22 inventory sites and corrected using capture-mark-recapture derived capture efficiencies. Mean abundance (10,000 model runs) and 90% confidence intervals (dotted lines) are shown. 16 4.5 Tributary species distribution Westslope cutthroat trout were captured in the majority of tributaries sampled, however most reaches were compromised of nonnative rainbow trout and hybrids. Pure populations of Westslope cutthroat trout remain restricted to a few select tributaries upstream of fish passage barriers. Tributaries with relatively homogenous cutthroat trout catches include: Island Creek, Giardi Creek, Star Creek, upper Blairmore Creek, upper Gold Creek, and upper Rock Creek (Figure 5 and Appendix 11). The remainder of tributaries sampled had various mixtures of invasive and native species. The Lyons Creek catch was dominated by rainbow trout, and Drum Creek was dominated by brook trout. Allison Creek was heavily impacted by brook trout, as well as lower Gold Creek. Mountain whitefish were captured only in the lower Allison Creek and Todd Creek watersheds. No bull trout were captured in any tributaries of the Crowsnest River drainage. 17 Figure 5. Species composition of electrofishing catch by tributary watershed in the Crowsnest River drainage in 2010. 18 4.6 Population structure and size distribution Rainbow trout and mountain whitefish in the Crowsnest River ranged widely in size (Table 4 and Figure 6); however the length-frequency distribution of rainbow trout was bimodal with a strong representation of small fish (80 mm to 180 mm). The mountain whitefish catch showed no definite trend in catch frequency by size, and a relatively homogenous representation across the full range of sizes (Figure 6). The difference in population structures potentially explains the discrepancy in total estimated abundances between the two species, if sampling bias toward small rainbow trout occurred. The majority of the brown trout and brook trout catch was represented by small fish in the 100 to 150 mm range, and 100 to 170 mm range, respectively. In the tributaries, cutthroat trout and hybrids attained the greatest size (Table 5), and length frequencies were similar between trout species, with the majority of the catch typically less than 260 mm (Figure 7). Table 4. Speciesa RNTR MNWH BNTR CRTR BKTR BLTR LKTR BURBa Size distribution of fish electrofished in the Crowsnest River in 2010. Fork length (mm) Mean SD Range 158 87 41-538 255 110 39-471 154 80 63-470 246 86 83-393 143 40 72-230 320 109 183-552 274 45 225-332 337 73 182-461 n 3,418 1,552 99 47 28 26 4 44 Mean 107 336 73 251 45 569 237 217 Weight (g) SD Range 226 1-1,727 345 1-1,280 170 3-1,183 198 9-637 44 9-160 666 68-2,400 91 153-334 119 32-487 n 2,761 1,338 89 38 27 20 3 36 RNTR = rainbow trout, MNWH = mountain whitefish, BNTR = brown trout, CRTR = cutthroat trout and cutthroat x rainbow trout hybrid combined, BKTR = brook trout, BLTR = bull trout, LKTR = lake trout, BURB = burbot a 19 25 Rainbow trout; n = 3,418 Cutthroat trout and hybrids; n = 47 20 15 10 5 Relative abundance (%) 0 25 Mountain whitefish; n = 1,547 Brook trout; n = 28 Brown trout, n = 99 Bull trout, n = 26 20 15 10 5 0 25 20 15 10 5 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 0 Fork length (mm) Figure 6. Length-frequency distributions of fish captured in the Crowsnest River in 2010. 20 Table 5. Size distribution of fish electrofished in tributaries to the Crowsnest River drainage in 2010. Fork length (mm) Species CTTR RNTR CRTR BKTR Weight (g) Mean SD Range n Mean SD Range n 138 139 141 148 51 40 42 50 42-325 53-258 63-325 74-281 513 309 191 169 45 42 39 48 54 36 45 43 1-390 1-222 2-426 4-241 401 297 174 123 CTTR = cutthroat trout, RNTR = rainbow trout, CRTR = cutthroat x rainbow trout hybrid, BKTR = brook trout a 21 20 Cutthroat trout; n = 513 Cutthroat x rainbow trout hybrids; n = 191 Rainbow trout; n = 309 Brook trout; n = 169 15 Relative abundance (%) 10 5 0 20 15 10 5 Fork length (mm) Figure 7. Length-frequency distributions of fish captured in the Crowsnest River tributaries in 2010. 22 330 300 270 240 210 180 150 120 90 60 30 0 330 300 270 240 210 180 150 120 90 60 30 0 0 4.7 Incidence of main-stem river hooking damage We observed evidence of hooking damage or angling related fish injury to rainbow trout at 17 of 22 reaches and at 8 of 21 reaches for mountain whitefish, in the main-stem river. The proportion of captured fish with evidence of hooking damage was higher for rainbow trout than for mountain whitefish (Table 6 and Appendix 12). At sample reaches where hooking damage to rainbow trout occurred, the proportion of hookdamaged fish tended to increase with fish size (Appendix 14). The proportion of rainbow trout and mountain whitefish with hooking damage was highest from sample reach 11, near East Hillcrest Bridge, to sample reach 14, near the Highway 507 crossing and coincided with reaches of high fish density. Table 6. Proportion (%) of the first pass catches of rainbow trout and mountain whitefish with evidence of hooking damage. Size range All fish > 300 mm TL > 400 mm TL > 450 mm TL Proportion hook-damaged rainbow trout (%) Mean SD Range 3 2 0-7 16 13 0-40 9 14 0-40 9 15 0-43 23 Proportion of hook-damaged mountain whitefish (%) Mean SD Range 3 4 0-13 3 4 0-13 3 5 0-15 4 6 0-20 4.8 Summary For the Crowsnest River, main-stem catches of rainbow trout far exceeded mountain whitefish catches, as did the resulting abundance estimates. The larger size classes of fish were very comparable in abundance, whereas only smaller (<300 mm) mountain whitefish were scarce. Fewer juvenile mountain whitefish may be a result of: 1) life history differences between the species, 2) sampling bias toward juvenile rainbow trout habitat, 3) interspecific competition, or 4) fewer juvenile mountain whitefish inhabited the main-stem river during the sampling period, perhaps seeking refuge in tributaries during the unusually high 2010 summer flows. Cutthroat trout populations in the tributaries were highest in Blairmore and Gold Creeks, with considerable portions of the two watersheds isolated from invasive rainbow trout. However, total tributary abundance estimates were a composite of all Oncorhynchids captured in the watersheds. Tributary populations of invasive brook trout were present, however a lack of capture efficiencies for brook trout precluded their abundance estimation. With such a high degree of species interspersion across the Crowsnest River watershed, accurate tributary abundance estimates by species would require much higher sampling intensities on a watershed by watershed basis. Fish densities were highest between the town of Bellevue and the Highway 507 bridge, coinciding with the areas of highest observed hooking damage, suggesting anglers and guides are aware of where fish densities are highest. The catch composition changed dramatically downstream of Lundbreck Falls from one dominated by rainbow trout and mountain whitefish, to a sucker dominated catch. It has long been suspected that the Oldman Reservoir provides excellent habitat for sucker species and few other species. Our catch results corroborate this theory, with Lundbreck Falls serving as an upstream migration barrier to large pods of longnose and white suckers. 24 5.0 LITERATURE CITED Alberta Sustainable Resource Development. 2010. 2010 Alberta guide to sportfishing regulations. Produced by the Government of Alberta, Edmonton, Alberta, Canada. 112 pp. Alberta Sustainable Resource Development. 2009. Alberta Westslope cutthroat trout recovery team update. Produced by the Government of Alberta, Edmonton, Alberta, Canada. Available at: http://www.srd.alberta.ca/BioDiversityStewardship/SpeciesAtRisk/RecoveryPro gram/RecoveryPlans.aspx. Alberta Sustainable Resource Development and Alberta Conservation Association. 2009. Status of bull trout (Salvelinus confluentus) in Alberta. Alberta Sustainable Resource Development Wildlife Status Report No. 39, Update 2009, Edmonton, Alberta, Canada. 48 pp. Alberta Sustainable Resource Development and Alberta Conservation Association. 2006. The status of Westslope cutthroat trout (Oncorhynchus clarkii lewisi) in Alberta. Alberta Sustainable Resource Development Wildlife Status Report No. 61, Edmonton, Alberta, Canada. 34 pp. Blackburn, J. 2010. Abundance and distribution of Westslope cutthroat trout in the Castle River drainage, Alberta 2008 - 2009. Technical Report, T-2010-002, produced by the Alberta Conservation Association, Lethbridge, Alberta, Canada. 39 pp + App. Faulter, R. 2003. Cooperative fisheries inventory program: 2003 survey site reference catalogue, Blairmore area. Produced by the Alberta Conservation Association, Blairmore, Alberta, Canada. 68 pp. Fisher, J. 2000. Cooperative fisheries inventory program, Atlas Lumber Timber Quota: 2000 catalogue of sampling sites. Produced by Atlas Lumber Ltd., Blairmore, Alberta, Canada. 99 pp. 25 Fitch, L. 1997. Bull trout in southwestern Alberta: notes on historical and current distribution. Pages 147-160. Proceedings. Alberta Environmental Protection, Fish and Wildlife Services, In: Friends of the Bull Trout Conference Lethbridge, Alberta, Canada. Fitzsimmons, K., and M. Blackburn. 2009. Abundance and distribution of Arctic grayling in the upper Little Smoky River, Alberta, 2007. Data Report, D-2009-004, produced by the Alberta Conservation Association, Cochrane, Alberta, Canada. 16 pp + App. Gabelhouse Jr., D. 1984. A length-categorization system to assess fish stocks. North American Journal of Fisheries Management 4: 273-285. Genereux, D.B., and M.B. Bryski. 2002. A creel survey of the Crowsnest River, June – September, 2001. Prepared for Alberta Environmental Protection, Natural Resources Service, Lethbridge, Alberta, Canada. 32 pp + App. Jokinen, M. 2002. Cooperative fisheries inventory program: 2002 survey site reference catalogue, Blairmore. Produced by the Alberta Conservation Association, Blairmore, Alberta, Canada. 97 pp. Krebs, C.J. 1999. Ecological Methodology, 2nd edition. Benjamin Cummings, Menlo Park, California, U.S.A. 620 pp. Lyons, J. 1992. The length of stream to sample with a towed electrofishing unit when fish species richness is estimated. North American Journal of Fisheries Management 12: 198-203. Muhlfeld C., S. Kalinowski, T. McMahon, M. Taper, S. Painter, R. Leary, and F. Allendorf. 2009. Hybridization rapidly reduces fitness of a native trout in the wild. Biology Letters 5: 328-331. doi: 10.1098/rsbl.2009.0033. R Development Core Team. 2009. computing. R: A language and environment for statistical Produced by R Foundation for Statistical Computing, Vienna, Austria. Available at: http://www.R-project.org. 26 Reynolds, L., A.T. Herlihy, P.R. Kaufmann, S.V. Gregory, and R.M. Hughes. 2003. Electrofishing effort requirements for assessing species richness and biotic integrity in western Oregon streams. North American Journal of Fisheries Management 23: 450-461. Robinson, M. 2007. The ecological consequences of hybridization between native Westslope cutthroat trout (Oncorhynchus clarkii lewisi) and introduced rainbow trout (Oncorhynchus mykiss) in south western Alberta. MSc thesis. University of Lethbridge, Lethbridge, Alberta, Canada. 152 pp. Schwartz, C. 2005. Statistics for resource managers: subset from STAT 403/650. Produced by the Department of Statistics and Actuarial Science, Simon Fraser University, Burnaby, British Columbia, Canada. 834 pp. Taylor, C. 2000. A large-scale comparative analysis of riffle and pool fish communities in an upland stream system. Environmental Biology of Fishes 58: 89–95. Taylor, E., and J. Gow. 2007. An analysis of hybridization between native Westslope cutthroat trout (Oncorhynchus clarkia lewisi) and introduced Yellowstone cutthroat trout, (O.c. bouvieri) and rainbow rrout (O. mykiss) in Canada’s mountain parks and adjacent watersheds in Alberta. Prepared for Parks Canada and Alberta Fish and Wildlife by the Native Fishes Research Group, University of British Columbia, Vancouver, British Columbia, Canada. 46 pp. 27 6.0 APPENDICES Appendix 1. Capture summary from Crowsnest River preliminary tote-barge eletrofishing in 2009. UTM coordinates NAD 83 Zone 11. 2010 Sample Mean UTM UTM Total Oncorhynchus Location distance wetted Easting Northing catch ID (m) width (m) Lundbreck Falls Campsite Lower NA 380a 30.0 702280 5496420 54 Burmis Road Middle 13 900 20.6 695315 5492275 193 East Hillcrest Bridge Middle 11 700 16.7 691890 5492041 208 Blairmore Highway 3 crossing Upper 6 820 17.4 686057 5498037 87 U/S Coleman at McGillvray Upper 2 600 16.5 678567 5500700 66 a Preliminary sampling near Lundbreck Falls was aborted because of unsafe river conditions. Although used in power analysis to estimate sampling intensity, it was not compiled with 2010 sample reaches to calculate main-stem abundance estimates. Preliminary sample reaches 2009 River reach 28 Appendix 2. Summary of cooperative fisheries inventory program data used in the Crowsnest River tributaries study design. CTTR = cutthroat trout, RNTR = rainbow trout, CRTR = cutthroat trout x rainbow trout hybrid. UTM coordinates NAD 83 Zone 11. Stream order Waterbody Year UTM Easting UTM Northing Sample distance (m) Mean wetted (m) Electrofishing effort (s) CTTR RNTR CRTR Total Oncorhynchus 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 Island Creek McGillvray Creek Rock Creek Star Creek Unnamed Blairmore Creek Blairmore Creek Byron Creek Drum Creek Green Creek Island Creek Lyons Creek Todd Creek Unnamed Unnamed Allison Creek Byron Creek Byron Creek Byron Creek Byron Creek Connelly Creek Connelly Creek Connelly Creek Cow Creek 2002 1983 2002 2003 2002 2000 2002 2002 2002 2002 2002 2002 2007 2002 2002 2002 2000 2002 2002 2002 2002 2003 2005 2005 665620 676643 695235 677781 695187 682916 682916 688466 690892 688982 666192 683989 693786 693594 690829 673600 691872 688640 691842 691868 695446 699390 695268 550858 5497668 5512036 5503305 5498885 5500871 5509937 5509929 5488723 5493157 5501258 5498419 5493855 5517492 5493099 5493029 5504300 5491997 5488881 5491972 5491993 5505161 5501473 5505261 5504093 300 100 300 300 300 100 300 200 300 300 300 300 200 300 300 300 185 300 300 200 300 300 200 700 1.9 na 1 2.8 2.7 2.7 2.7 3.7 3.3 1.9 2.8 3.8 2.6 1.8 2.8 3.7 4.9 3.7 4.9 4.9 1.6 2.4 1.6 1.3 597 na 193 581 746 120 699 549 718 423 614 732 400 634 636 450 na 729 884 na 610 759 974 na 12 12 0 30 25 40 59 0 0 0 3 0 15 0 0 14 0 0 0 0 17 11 1 na 10 0 0 1 0 0 1 0 13 0 0 10 0 14 2 0 25 0 24 30 0 0 0 na 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 0 0 0 0 2 1 22 12 0 31 25 40 60 0 13 0 3 10 15 14 2 14 30 0 24 30 17 11 3 1 29 Appendix 2. Continued Stream order Waterbody 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 Lyons Creek Lyons Creek Lyons Creek Lyons Creek Lyons Creek Lyons Creek Morin Creek South Todd Creek South Todd Creek Star Creek Star Creek Star Creek Star Creek Star Creek Todd Creek Todd Creek Todd Creek Todd Creek Todd Creek Unnamed York Creek York Creek York Creek York Creek York Creek Year UTM Easting UTM Northing Sample distance (m) Mean wetted (m) Electrofishing effort (s) CTTR RNTR CRTR Total Oncorhynchus 2002 2002 2002 2003 2004 2006 2002 2000 2001 2002 2002 2002 2002 2003 2000 2000 2000 2000 2002 2002 2002 2002 2002 2003 2003 684212 684214 684953 684027 684210 684216 688051 695411 695348 677498 677755 677759 677708 677781 694404 694898 695671 695185 695658 673016 681305 683113 683117 681400 683109 5494123 5494111 5497598 5493920 5494109 5494099 5502999 5515936 5515899 5500357 5499722 5499759 5500006 5499231 5518542 5517912 5516413 5517078 5516457 5499006 5497942 5498222 5498237 5497991 5498449 300 300 300 300 300 300 300 123 300 150 300 250 200 300 150 100 155 150 300 300 100 100 100 300 300 5.4 5.4 6.8 3.8 5.4 5.4 2.7 2.2 2.2 3.4 4.2 4.2 5.7 4 2.5 2.6 3.3 4.4 3.3 1.9 5.3 6 6.2 5.3 7.3 979 1443 1391 3147 1764 931 538 1000 840 560 696 763 544 441 288 277 1500 658 755 596 314 221 244 1035 981 0 0 0 0 0 0 16 62 69 5 20 1 1 0 21 24 103 46 16 30 4 0 2 2 3 43 86 31 29 95 56 0 0 2 4 0 3 2 0 0 0 0 3 16 0 0 2 4 6 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 3 0 0 0 0 0 0 43 86 31 29 95 56 16 62 71 9 20 4 3 0 21 24 110 49 35 30 4 2 6 8 37 30 Appendix 2. Continued Stream order Waterbody 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Allison Creek Allison Creek Blairmore Creek Cow Creek Cow Creek Crowsnest Creek Crowsnest Creek Gold Creek Gold Creek Gold Creek Gold Creek Gold Creek Rock Creek Rock Creek South Todd Creek Todd Creek Todd Creek Todd Creek Year UTM Easting UTM Northing Sample distance (m) Mean wetted (m) Electrofishing effort (s) CTTR RNTR CRTR Total Oncorhynchus 1997 2000 2002 2005 2005 2003 2008 2000 2002 2002 2006 2006 2002 2007 2006 2000 2002 2007 674335 673822 683662 554498 552475 668245 669171 688807 687808 688157 687493 688621 695691 693812 695554 705555 707073 696884 5499838 5503812 5501943 5503409 5503813 5499381 5499936 5501542 5505931 5502565 5496613 5498644 5501050 5501035 5516108 5509059 5505880 5516341 450 275 300 300 700 200 200 400 600 300 850 1000 na na 400 200 300 100 4.6 3.7-4.5 4.5 <3 <3 >5 >5 >5.8 5.4 5.8 7.6 7.6 2.7 na 2.2 4.8 4.1 3.3 1338 450 633 na na 1038 1061 960 2218 457 na na 578 2294 673 710 704 350 2 4 20 0 0 1 0 30 140 13 4 28 18 16 0 1 0 1 16 0 6 0 0 1 0 0 0 0 9 0 0 0 0 63 0 0 0 4 16 12 2 0 0 0 0 0 17 2 0 0 49 0 0 0 18 8 42 12 2 2 0 30 140 13 30 30 18 16 49 64 0 1 31 Appendix 3. Crowsnest River sample site locations in 2010. UTM coordinates NAD 83 Zone 11. Location ID Start Easting Start Northing End Easting End Northing Sample date Km upstream of Todd Creek 22 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 20 21 19 672501 674413 677171 678567 681039 683515 686057 687081 688840 689859 691066 691890 693964 695315 696540 698320 699342 699224 701201 703505 705569 706669 5500660 5499725 5500622 5500700 5500615 5498780 5498037 5497179 5495752 5495018 5492899 5492041 5492141 5492275 5492572 5493494 5493562 5495342 5495452 5497346 5497359 5497552 673021 675003 677821 678972 681492 683920 686793 687469 689332 690375 691337 692195 694575 695749 697201 698537 699331 699873 701698 704758 706031 706822 5500649 5499730 5500721 5500618 5500359 5498717 5498006 5496620 5495422 5494860 5492514 5491953 5491981 5492282 5492789 5493738 5493944 5495350 5495831 5497287 5497463 5497904 11-Jun-10 5-Aug-10 17-Aug-10 9-Aug-10 10-Aug-10 16-Aug-10 5-Aug-10 3-Aug-10 4-Aug-10 28-Jul-09 11-Aug-10 30-Jul-09 28-Jul-10 28-Jul-10 22-Jul-10 17-Jul-10 22-Jul-09 20-Jul-10 20-Jul-10 12-Jul-10 14-Jul-10 22-Jul-09 54.0 51.1 46.8 45.5 42.7 39.5 36.3 34.3 31.9 30.6 27.9 26.3 22.1 19.9 18.1 15.5 13.8 11.1 8.7 4.7 2.8 1.7 32 Lundbreck Falls Appendix 4. Crowsnest River tributary sample site locations in 2010. UTM coordinates NAD 83 Zone 11. Location ID 1 2 3 6 8 9 10 11 12 13 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Waterbody name Unnamed tributary to Lyons Creek Unnamed tributary to Lyons Creek Drum Creek Todd Creek Unnamed tributary to Todd Creek South Todd Creek Todd Creek Cow Creek Cow Creek Gold Creek Caudron Creek Unnamed tributary to Blairmore Creek Blairmore Creek Gold Creek McGillivray Creek McGillivray Creek Star Creek York Creek Allison Creek Allison Creek Unnamed tributary to Allison Creek Gold Creek Rock Creek Rock Creek Todd Creek Start Easting 683582 684879 689194 695704 693456 694939 703499 698945 695338 687772 688240 683757 684495 689091 677479 677450 677645 682951 673999 672517 672501 687803 694135 699794 693129 33 Start Northing 5492002 5496072 5493922 5516438 5516187 5514512 5513715 5508877 5509254 5505674 5506701 5506604 5507448 5500053 5506536 5509225 5499181 5498071 5502567 5507041 5505427 5497602 5501133 5496348 5519569 End Easting 683596 684991 689049 695567 693230 694842 703347 698746 695095 687795 688473 683765 684467 701698 677532 677372 677387 682930 673933 672508 672335 687914 693921 699739 692930 End Northing 5491831 5495858 5493771 5516587 5516188 5514318 5513941 5508985 5509194 5505977 5506816 5506857 5507743 5495831 5506752 5509479 5499101 5497821 5502741 5507235 5505636 5497844 5501075 5496308 5519795 Sample date 30-Jun-10 24-Jun-10 6-Jul-10 13-Jul-10 13-Jul-10 13-Jul-10 13-Jul-10 28-Jun-10 28-Jun-10 7-Jul-10 7-Jul-10 2-Sep-10 6-Jul-10 29-Jul-10 5-Jul-10 23-Jun-10 8-Jul-10 18-Aug-10 6-Jul-10 6-Jul-10 22-Jun-10 22-Jul-10 18-Aug-10 18-Aug-10 13-Jul-10 Appendix 4. Continued. Location ID 31 32 33 34 35 37 38 39 40 41 42 43 45 49 52 59 60 Waterbody name Allison Creek Blairmore Creek Nez Pierce Creek Connelly Creek Connelly Creek Connelly Creek Connelly Creek Blairmore Creek Island Creek McGillivray Creek Rock Creek Island Creek Todd Creek Unnamed Creek (Fools Creek) Giardi Creek Unnamed tributary to Rock Creek Byron Creek Start Easting 672227 683564 679698 693751 695694 696233 700374 683490 665326 678645 697338 666117 699766 676428 672659 695691 690317 34 Start Northing 5512100 5508993 5504643 5506627 5504609 5504182 5500400 5501435 5498100 5503126 5498528 5498952 5515503 5501232 5499866 5499737 5489915 End Easting 672194 683361 679700 693508 695532 696053 700172 683596 665129 678389 697137 666128 699656 676330 672728 695445 690041 End Northing 5512381 5509166 5504873 5506713 5504436 5504211 5500408 5501672 5498238 5503126 5498658 5498717 5515673 5501496 5499615 5499707 5489829 Sample date 22-Jun-10 5-Jul-10 23-Jun-10 29-Jun-10 29-Jun-10 1-Sep-10 1-Sep-10 26-Jul-10 23-Jun-10 8-Jul-10 18-Aug-10 23-Jun-10 13-Jul-10 24-Jun-10 22-Jun-10 30-Jun-10 18-Aug-10 Appendix 5. Crowsnest River measurement data in 2010. WW = wetted width, RW = rooted width, SD = standard deviation, s = seconds. Location ID Mean WW (m) SD WW Mean RW (m) SD RW Mean thalweg depth (m) SD thalweg depth % Pool % Riffle % Run Electrofishing effort (s) Distance fished (m) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 15.5 16.0 16.5 13.0 10.2 17.4 18.5 20.0 15.7 23.6 16.7 18.4 20.6 21.3 19.8 23.3 22.4 25.9 20.8 23.8 22.0 15.1 2.3 1.7 2.3 1.7 2.0 2.0 2.9 1.7 4.5 4.8 3.6 1.8 3.4 2.4 3.5 4.2 5.4 2.8 2.5 3.9 3.8 3.4 17.1 18.0 22.2 17.2 12.4 18.4 23.0 22.7 17.3 26.5 25.4 26.4 23.4 25.4 24.6 37.4 30.9 31.1 29.6 26.4 26.1 16.4 1.6 1.4 4.0 4.5 1.5 2.1 3.3 2.5 4.7 6.6 10.5 4.4 4.8 2.2 2.8 16.8 10.2 4.1 6.2 2.6 6.2 3.7 0.79 1.07 0.73 0.58 1.06 1.19 1.04 0.67 1.45 1.11 0.94 1.29 0.86 0.56 0.68 1.31 0.89 1.13 1.25 0.56 1.14 0.99 0.18 0.73 0.41 0.16 0.17 0.26 0.51 0.15 0.93 0.48 0.56 0.66 0.42 0.18 0.31 1.15 0.42 0.50 0.61 0.29 0.40 0.24 31 8 34 4 0 19 18 4 38 21 20 28 7 19 32 31 16 16 30 10 25 14 24 67 3 32 100 33 18 81 38 37 54 40 32 42 36 40 53 61 41 52 43 25 45 25 63 64 0 49 64 14 23 42 26 33 61 39 32 29 31 23 29 38 33 61 2243 2758 2951 1625 1698 4194 4228 2486 2742 2238 3045 6545 3653 4887 3489 3440 4114 3192 3767 4098 3888 2511 700 700 600 500 500 820 750 700 600 800 700 800 900 800 800 800 800 700 800 800 800 700 35 Appendix 6. Crowsnest tributary stream measurement data in 2010. WW = wetted width, RW = rooted width, SD = standard deviation, s = seconds. Location ID Mean WW (m) SD WW Mean RW (m) SD RW Mean thalweg depth (m) SD thalweg depth % Pool % Riffle % Run Electrofishing effort (s) Distance fished (m) 1 2 3 6 8 9 10 11 12 13 15 16 17 18 19 20 21 22 23 24 25 26 3.4 1.6 na 2.6 1.3 2.1 5.1 3.5 1.9 5.9 3.3 1.5 4.6 7.0 4.0 4.9 4.8 5.4 4.8 3.6 2.0 5.2 0.7 0.4 na 0.3 0.3 0.6 0.8 1.1 0.5 1.1 0.7 0.3 0.8 1.5 0.9 1.0 2.7 0.8 0.7 0.5 0.5 1.1 5.0 2.8 na 6.9 2.5 3.2 8.9 4.6 2.4 7.5 4.6 3.2 6.4 8.8 5.7 6.8 6.2 7.1 5.9 12.8 3.5 7.4 0.7 1.0 na 4.4 0.9 0.7 0.8 1.3 1.1 1.4 1.0 1.0 1.2 1.6 2.3 1.4 2.7 0.8 1.1 19.7 1.3 1.6 0.30 0.24 na 0.41 0.16 0.40 0.47 0.46 0.74 0.45 0.45 0.13 0.32 0.47 0.32 0.59 0.50 0.52 0.33 0.38 0.15 0.44 0.11 0.06 na 0.30 0.06 0.15 0.13 0.11 0.17 0.07 0.04 0.04 0.10 0.24 0.04 0.09 0.54 0.22 0.08 0.12 0.04 0.06 18 22 0 23 18 2 20 18 1 0 5 25 7 7 40 3 8 22 8 7 19 2 49 53 80 45 35 19 42 52 0 100 95 17 72 80 52 97 68 42 87 78 73 92 33 25 20 32 47 79 38 30 99 0 0 58 22 13 8 1 23 37 5 15 8 7 1716 666 1218 1175 1264 1326 1285 1412 1489 2035 764 1596 1482 1372 1569 935 1877 2223 1852 1577 1001 1239 300 300 300 300 300 300 300 300 300 300 300 300 300 300 250 300 300 300 300 300 300 300 36 Appendix 6. Continued. Location ID Mean WW (m) SD WW Mean RW (m) SD RW Mean thalweg depth (m) SD thalweg depth % Pool % Riffle % Run Electrofishing effort (s) Distance fished (m) 27 28 29 31 32 33 34 35 37 38 39 40 41 42 43 45 49 52 59 2.5 3.9 2.5 1.2 3.4 3.6 1.1 1.3 1.4 2.9 4.8 2.1 4.9 3.5 3.0 4.5 2.4 2.8 1.2 1.0 1.2 0.5 0.5 1.2 0.5 0.5 0.3 0.2 1.2 0.5 0.4 0.5 1.0 0.7 1.3 0.8 1.0 0.2 3.0 5.1 3.1 2.0 5.4 5.2 2.0 2.3 1.5 4.0 7.6 2.6 7.8 5.4 3.9 8.8 2.5 3.4 1.6 0.9 1.1 0.4 1.6 0.6 0.7 0.9 1.8 0.5 1.0 1.7 0.7 1.8 1.3 0.9 5.2 0.8 1.0 0.4 0.26 0.22 0.28 0.12 0.31 0.33 0.20 0.30 0.31 0.23 0.21 0.26 0.31 0.21 0.32 0.67 0.30 0.56 0.15 0.06 0.05 0.07 0.04 0.09 0.05 0.08 0.09 0.07 0.14 0.08 0.06 0.06 0.05 0.09 0.65 0.07 0.71 0.04 32 29 3 22 15 5 15 17 0 24 5 23 23 15 16 2 2 13 5 68 43 5 73 67 91 65 14 1 26 77 65 68 44 83 21 8 82 60 0 28 92 5 18 4 20 69 99 50 18 13 9 41 1 78 91 6 35 1509 968 996 646 1663 895 676 1328 1212 1651 2093 874 962 1388 957 1321 761 1478 894 300 300 300 300 300 300 300 250 300 300 300 300 300 300 300 300 300 300 300 37 Appendix 7. Fish catch at 2010 sampling sites used to estimate abundance by size class. TL = total length, RNTR = rainbow trout, MNWH = mountain whitefish. Bold script denotes sampling conducted in 2009. All fish Location Id RNTR MNWH RNTR MNWH RNTR MNWH RNTR MNWH Distance from Todd Creek (m) 24 47 79 178 207 85 196 167 192 335 208 222 147 132 81 79 60 40 66 20 30 50 4 16 16 11 8 17 24 33 33 27 32 19 11 4 7 7 1 4 3 0 5 8 2 4 25 0 19 33 41 30 59 91 39 24 29 7 47 36 9 12 14 10 17 0 0 6 7 9 6 14 21 26 28 21 13 14 5 2 2 2 0 0 0 0 2 7 0 1 0 0 17 32 36 26 50 75 22 17 16 0 3 0 0 1 2 0 0 0 0 1 0 5 5 7 7 8 6 6 1 2 0 0 1 0 0 0 0 0 0 2 0 0 0 0 6 10 9 10 14 11 0 0 2 0 0 0 0 0 0 0 0 0 1,664 2,847 4,677 8,732 11,115 13,762 15,461 18,107 19,877 22,140 26,329 27,897 30,570 31,863 34,296 36,307 39,482 42,686 45,480 46,836 51,139 54,006 19 21 20 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 22 30 24 82 34 27 36 45 38 90 102 43 29 35 18 116 196 32 60 62 46 56 0 >300 mm TL >400 mm TL 38 >450 mm TL Appendix 8. Sport fish catch at 2010 tributary sample sites used to determine corrected abundance per site. CTTR = cutthroat trout, CRTR = rainbow trout x cutthroat trout hybrid, RNTR = rainbow trout, MNWH = mountain whitefish, BKTR = brook trout. Location ID 1 2 3 6 8 9 10 11 12 13 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 31 32 33 34 35 37 38 39 40 41 42 Date 30-Jun-10 24-Jun-10 6-Jul-10 13-Jul-10 13-Jul-10 13-Jul-10 13-Jul-10 28-Jun-10 28-Jun-10 7-Jul-10 7-Jul-10 2-Sep-10 6-Jul-10 29-Jul-10 5-Jul-10 23-Jun-10 8-Jul-10 18-Aug-10 6-Jul-10 6-Jul-10 22-Jun-10 22-Jul-10 18-Aug-10 18-Aug-10 13-Jul-10 22-Jun-10 5-Jul-10 23-Jun-10 29-Jun-10 29-Jun-10 1-Sep-10 1-Sep-10 26-Jul-10 23-Jun-10 8-Jul-10 18-Aug-10 CTTR 0 CRTR RNTR MNWH 120 0 0 0 no fish captured 0 0 0 0 2 6 4 34 10 0 13 0 0 0 0 6 1 29 1 72 71 29 13 9 0 0 0 0 0 0 0 0 0 0 0 0 0 6 37 5 0 0 0 1 0 9 0 no fish captured no fish captured 0 0 0 10 16 0 19 11 0 2 0 0 1 0 0 3 0 0 0 0 0 1 0 0 no fish captured no fish captured 2 0 0 no fish captured no fish captured 2 0 0 3 0 0 8 5 0 74 39 0 no fish captured 1 0 0 4 23 0 28 1 0 7 0 6 34 3 70 0 6 0 5 1 1 39 BKTR 0 48 0 0 0 0 0 0 0 0 0 0 29 0 0 30 0 38 3 0 0 0 0 0 0 16 0 0 Appendix 8. Continued. Location ID 43 45 49 52 59 60 Date 23-Jun-10 13-Jul-10 24-Jun-10 22-Jun-10 30-Jun-10 18-Aug-10 Total CTTR 9 0 CRTR RNTR MNWH 1 0 0 10 1 0 no fish captured 0 0 0 no fish captured no fish captured 36 460 155 40 290 16 BKTR 0 0 0 116 Appendix 9. Summary of Peterson capture-mark-recapture (CMR) capture probabilities (q) used to correct 2010 single-pass backpack electrofishing catch. 502 138 118 93 357 104 572 176 55 Lower 95% CI 379 107 88 69 285 84 376 133 46 Upper 95% CI 747 200 186 151 484 140 1111 270 77 0.2888 0.4348 0.4153 0.4409 0.3782 0.5385 0.1556 0.4150 0.6364 166 241 105 121 201 65 277 310 271 0.4036 0.4772 0.2762 Waterbody M C R E Daisy 2007 Daisy 2007 Dutch 2007 Racehorse 2007 Station 2007 Tributary to Racehorse 2007 Lynx 2009 Carbondale 2010 Lost 2010 145 60 49 41 135 56 89 73 35 96 61 47 40 102 60 89 52 42 27 26 19 17 38 32 13 21 26 Upper Blairmore 2010 67 41 Lower Blairmore 2010 Upper Gold 2010 115 29 102 28 16 48 7 41 q Appendix 10. Summary of Jolly-Seber capture-mark-recapture probabilities used to correct 2010 main-stem Crowsnest River single-pass tote-barge electrofishing catches. Pass 1 Pass 2 Pass 3 Pass 1 Pass 2 Pass 3 2nd pass recaps from 1st pass 21 85 197 84 51 18 97 185 87 41 18 68 145 141 27 21 81 193 78 49 18 95 175 81 39 18 58 139 141 27 2 11 18 5 6 3 2 8 5 2 1 7 4 12 4 0 0 2 1 1 193 (48-1,931) 286 (136-1,234) 2,638 (1,213-8,826) 589 (265-2,178) 156 (70-675) 0.1580 0.1220 0.1020 0.0680 0.1670 0.2630 0.1450 0.1160 0.1410 0.3210 Mountain Whitefish 2 46 7 116 15 43 20 81 22 0 22 88 27 76 4 34 131 11 94 4 44 114 41 55 0 22 88 27 55 4 34 126 11 90 4 8 14 8 2 na 3 23 0 3 na 2 19 2 2 na 0 0 0 0 na 79 (35-371) 690 (417- 1,520) 25 (25-221) na na 0.3910 0.1690 0.3210 na na 0.1710 0.3260 0.2500 na na Total captures Location Id Rainbow Trout 2 7 15 20 22 Less deaths 3rd pass recaps from 1st pass 3rd pass recaps from nd 2 pass Captured 3 consecutive passes Abundance estimate (95% CI) Alpha 1 Alpha 2 42 Appendix 11. Electrofishing catch at 2010 sampling sites used to describe species distributions in the Crowsnest River. Bolded rows denote 2009 preliminary sampling. Location ID Upstream from Todd Creek (km) All fisha RNTR MNWH BKTR BLTR BNTR CRTR CTTR LKTR BURB LNSC WHSC 19 21 20 1.7 2.8 4.7 24 47 79 30 24 82 0 0 0 2 4 8 1 4 1 0 9 4 1 0 2 0 0 0 2 6 20 33 66 77 22 84 18 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 22 8.7 11.1 13.8 15.5 18.1 19.9 22.1 26.3 27.9 30.1 31.9 34.3 36.3 39.5 42.7 45.8 46.8 51.1 54.0 Total 115 244 291 178 34 0 0 0 0 0 0 0 0 0 212 207 27 0 0 0 0 0 0 0 0 0 234 85 36 0 0 0 1 0 1 0 0 0 123 196 45 0 0 0 1 0 0 0 0 0 242 167 38 0 0 1 0 0 0 0 0 0 206 192 90 0 0 0 0 0 0 0 0 0 282 335 102 0 0 1 1 0 0 0 0 0 439 208 43 0 0 0 0 0 0 0 0 0 251 222 29 0 0 0 0 0 0 0 0 0 251 147 35 0 0 0 2 0 0 0 0 0 184 132 18 0 0 2 3 0 0 0 1 0 156 81 116 2 0 2 2 2 1 0 0 0 206 79 196 1 0 3 5 0 1 0 1 0 286 60 32 0 0 2 2 0 0 0 1 0 97 40 60 1 0 2 0 0 0 0 3 1 107 66 62 4 0 28 0 0 0 0 3 1 164 20 46 2 0 10 0 1 0 0 3 0 82 30 56 3 0 4 0 0 0 0 3 0 96 50 0 4 0 0 1 0 0 0 8 0 63 Total 2,645 1,201 17 14 61 31 6 3 28 199 126 4,331 aRNTR = rainbow trout, MNWH = mountain whitefish, BKTR = brook trout, BLTR = bull trout, BNTR = brown trout, CRTR = cutthroat x rainbow trout hybrid, CTTR = cutthroat trout, LKTR = lake trout, BURB = burbot, LNSC = longnose sucker, WHSC = white sucker 43 Falls Appendix 12. Proportion (%) of the 2010 first pass electrofishing catch of rainbow trout and mountain whitefish, by sampling site and size range (mm), with evidence of hooking damage. Proportion hook-damaged Proportion hook-damaged rainbow trout (%) mountain whitefish (%) Location ID 1 All >300 >400 >450 All >300 >400 >450 fish TL TL TL fish TL TL TL 7 40 0 na 2 no hooking damage no hooking damage no hooking damage 3 5 33 na na no hooking damage 4 3 25 na na no hooking damage 5 no hooking damage no hooking damage 6 1 14 0 0 7 1 14 0 0 no hooking damage 8 1 25 0 na no hooking damage 9 2 18 40 0 10 1 5 7 0 11 6 31 40 0 7 8 9 na 12 1 7 10 22 4 4 3 8 13 3 9 10 13 4 7 6 7 14 7 24 24 43 13 13 15 0 11 12 14 10 9 9 9 20 15 16 no hooking damage 4 17 18 14 29 2 3 9 3 na 0 0 no hooking damage no hooking damage no hooking damage 18 3 36 33 40 no hooking damage 19 4 33 na na no hooking damage 20 5 19 0 na no hooking damage 21 5 0 0 0 no hooking damage 22 na no hooking damage na 44 na na na The Alberta Conservation Association acknowledges the following partner for their generous support of this project