Unique and reliable detection and quantification of species DNA

Unique and reliable detection and
quantification of species DNA
In collaboration with Imegen*, we are offering a broad portfolio of real-time
PCR based species detection and quantification testing solutions, with very high
sensitivity below the officially required limit of detection (LOD) of 0,1% (ref. EU No
51/2013 of 16 Jan 2013, amending reg. EU No 152/2009):
Full workflow solution
Very high sensitivity
Quantitative
• DNA extraction with high sample input
• Detection of mitochondrial DNA
• Quantification based on DNA standards
• Species DNA/total animal DNA result (%)
To learn more about our food testing solutions go to:
lifetechnologies.com/foodsafety
*Imegen = Instituto de Medicina Genomica, a highly experienced and professional company in genetics, Valencia Spain
DNA extraction
Detection of
species DNA
Quantification of
species DNA
Same day results
species DNA/animal DNA
Extraction
Detection
GMO Extraction kit
RapidFinder™ ID kit
The GMO Extraction
Kit provides a fast and
easy silica-based DNA
purification method to obtain
DNA from a wide variety
of food samples, like raw
materials and processed
food.
The RapidFinder™ ID kit
detects mitochondrial
DNA** in food samples.
Each kit contains a positive
control consisting of 0,1%
species DNA. All reagents
for 48 reactions are
provided.
Characteristics:
Characteristics:
High sample input: up to
20g of sample (homogenize
sample)
Easy to Use: real-Time PCR,
no electrophoresis
Safe: no toxic reagents
Reliable: inhibitors removal,
isolation of high pure DNA
(A260/A280 > 1.8)
Easy to use: minimal
handling steps
Reliable: internal Positive
Control (IPC) allows ruling
out inhibitors during PCR
Highest sensitivity: limit of
detection of 0.01% (w/w) in
fresh meat
Quantification
RapidFinder™ Quant
Multi Meat Set
The RapidFinder™ Quant Set
kit detects mitochondrial
DNA** in food samples and
includes the standards for
quantification (species and
animal).***
All reagents for 48 reactions
are provided.
Characteristics:
Easy to Use: real-Time PCR,
no electrophoresis
Reliable: internal Positive
Control (IPC) allows ruling
out inhibitors during PCR
Highest sensitivity: limit of
detection of 0.05% (w/w) in
fresh meat
**Mitochondrial DNA (mtDNA) is a multi-copy DNA target, as mammalian cells contain several copies of mtDNA per cell. The RapidFinder™ ID kits and RapidFinder™
Quant multi meat set, detect mitochondrial DNA, giving the assay a very high sensitivity and making the assay applicable to be used with processed food.
***Quantification assay is based on two absolute quantifications: the total amount of species DNA (using RapidFinder™ species ID Kit and RapidFinder™ Quant MultiMeat Set), and the total amount of animal present in one sample using the RapidFinder™ Quant Multi-Meat Set.
Product overview
Description
Cat. No.
Volume
GMO Extraction kit
4466336
48 rxn
RapidFinder Beef ID kit
A24391
48 rxn
Bos taurus
RapidFinder Pork ID kit
A24392
48 rxn
Sus Scrofa
™
RapidFinder Chicken ID kit
A24393
48 rxn
Gallus gallus
RapidFinder Turkey ID kit
A24394
48 rxn
Meleagris gallopavo
RapidFinder™ Sheep ID kit
A24395
48 rxn
Ovis aries
RapidFinder™ Poultry ID kit
A24397
48 rxn
Several avian species
RapidFinder Fish ID kit
A24398
48 rxn
Broad scope of fish species
RapidFinder Quant Multi Meat Set
A24399
48 rxn
™
™
™
™
™
Species name
Selection of validation data
Imegen has performed validation studies for which reports are available upon request.
Specificity assay of the RapidFinder™
Chicken ID kit.
Specificity assay of the RapidFinder™
Beef ID kit.
Analyses were performed using DNA from pure meat.
Analyses were performed using DNA from pure meat.
Meat Species
Results
Meat Species
Results
Horse
Not detected
Horse
Not detected
Mule
Not detected
Mule
Not detected
Donkey
Not detected
Donkey
Not detected
Beef
Not detected
Beef
Detected
Swine
Not detected
Swine
Not detected
Buffalo
Not detected
Buffalo
Not detected
Deer
Not detected
Deer
Not detected
Chicken
Detected
Chicken
Not detected
Turkey
Not detected
Turkey
Not detected
Duck
Not detected
Duck
Not detected
Ostrich
Not detected
Ostrich
Not detected
Goose
Not detected
Goose
Not detected
Human
Not detected
Human
Not detected
Repeatability assay for the RapidFinder™
Chicken ID kit.
Repeatability assay for the RapidFinder™
Beef ID kit.
Analyses were performed using DNA from pure meat.
Analyses were performed using DNA from pure meat.
The standards used were mixes with 0,1% chicken/
turkey, 0,01% chicken/turkey and 0,1% chicken/swine.
Seven replicates of three different samples of DNA
extracted from meat have been analysed.
The standards used were mixes of 0.1% beef/pork,
0.01% beef/pork, 0.01% beef/horse. Seven replicates
of three different samples of DNA extracted from meat
have been analysed.
Standard used
Technician 1
Technician 2
Standard used
Technician 1
Technician 2
0,1% chicken/turkey
100% (7/7)
100% (7/7)
0,1% chicken/turkey
100% (7/7)
100% (7/7)
0,01% chicken/turkey
100% (7/7)
100% (7/7)
0,01% chicken/turkey
100% (7/7)
100% (7/7)
0,1% chicken/swine
100% (7/7)
100% (7/7)
0,1% chicken/swine
100% (7/7)
100% (7/7)
The kits are optimised
for use in all Applied
Biosystems® Real-Time
PCR Instruments.
Applied Biosystems® 7500
Fast Real-Time PCR Instrument
Applied Biosystems® StepOne™ Real-Time PCR Instrument
Maximise results. Minimise risk.
When there’s no room for error, you need to work with someone
that’s as committed to risk reduction as you are. We combine fast,
accurate, and innovative food safety technologies with dedicated
global service and support to protect your brand and your bottom
line. From customised PCR assays and reagents to off-the-shelf food
testing solutions for Salmonella, Salmonella Enteritidis, E. coli, Listeria,
Cronobacter sakazakii, and Campylobacter jejuni, no one is better
positioned to meet your unique food testing needs.
To learn how we can make a difference in your lab, visit:
lifetechnologies.com/foodsafety
FOR TESTING OF FOOD AND ENVIRONMENTAL SAMPLES ONLY.
Life Technologies is a Thermo Fisher Scientific brand. © 2014 Thermo Fisher Scientific, Inc. All rights reserved. The Imegen logo is a registered
trademark of Dimigen, S.L. All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries. CO116894 0314
*Imegen = Instituto de Medicina Genomica, a highly experienced and professional company in genetics, Valencia Spain
Identificación y cuantificación de especies
Detecte y cuantifique ADN de distintas especies animales con precisión.
Detección de ADN mitocondrial en muestras de carne. (0,01% p/p en carne fresca)
Resultados cuantitativos (en combinación con el Animal Quant set) (0,05% p/p)
Kits optimizados para todos los equipos de PCR en tiempo real de Applied Biosystems
Producto
TaqMan®Beef ID Kit
TaqMan®Pork ID Kit
TaqMan®Poultry ID Kit
TaqMan®Chicken ID Kit
TaqMan®Turkey ID Kit
TaqMan®Animal Quant Set
RapidFinderTM Equine ID Kit
RapidFinderTM Equine Quant Set
Formato
48 rxn
48 rxn
48 rxn
48 rxn
48 rxn
48 rxn
48 rxn
48 rxn
PN
ESAPA002-IMG162
ESAPA002-IMG163
ESAPA002-IMG165
ESAPA002-IMG168
ESAPA002-IMG169
ESAPA002-IMG166
A15570
A15579
Productos relacionados
GMO Extraction kit
TaqMan®Sheep ID Kit
TaqMan®Ruminant ID Kit
Formato
50 test
48 rxn
48 rxn
PN
4466336
ESAPA002-IMG171
ESAPA002-IMG170
Contacte con nosotros: [email protected]
Para análisis de alimentos