Document 6428680

PO Box 2074
Kelvin Grove Qld 4059
Australia
T +61 7 3129 8450
F +61 7 3838 9413
E-mail: [email protected]
www.rcpaqap.com.au/transfusion
ABN 32 003 520 072
Transfusion
TEST and TEACH PROGRAM
2012-1 QUESTIONS
The following questions were taken from various sources and designed to not only test and teach but also encourage laboratory
staff to further their knowledge in the field of immunohaematology.
It is recommended that all staff actively working in the blood bank laboratory perform this exercise, initially without the benefit of
the answers. Use the table to record your answers and score. Most answers are referenced so participants may read further if
interested. The completed document should be filed as evidence of on-going education.
THIS IS AN EDUCATIONAL EXERCISE - PLEASE DO NOT RETURN RESULTS
Answers Guide - Select one (1) answer option only, i.e. only one of the options given is correct.
1.
With respect to anti-IH select the most appropriate response.
a. This is a benign usually cold reacting antibody found in group A1, B and A1B individuals that has never been
associated with a haemolytic transfusion reaction
b. This is an antibody found in individuals with the Bombay and para-Bombay phenotype that is responsible with severe
haemolytic transfusion reactions
c. This is a benign usually cold reacting antibody found in group A1, B and A1B individuals that has been reported to
cause a severe haemolytic transfusion reaction
d. This is a benign usually cold reacting antibody found in group O individuals that has been reported to cause a severe
haemolytic transfusion reaction
2.
In the investigation of Delayed Haemolytic Transfusion Reaction, crossmatching the post-transfusion patient’s plasma
against the original donor units may be helpful in establishing the diagnosis.
a. False
b. True
3.
Insert the appropriate A subgroup against the following reactions.
A1
A2
A3
Ax
Ael
1
2
3
4
5
Anti-A
++++
0
++(+) mf
++++
0/+
Anti-A,B
++++
0
++(+) mf
++++
++(+)
Anti-A1
++++
0
0
0
0
Anti-H
0
++++
++++
++++
++++
Substance in Saliva
A,H
H
A,H
A,H
H
Anti-A1in serum
No
Sometimes
Sometimes
Sometimes
Often
Subgroup
Reproduced from Storry, J.R. et al, 2009, ‘The ABO blood group system revisited: a review and update’, Immunohaematology, vol 25, pp 48-59
4.
Patients with common type 1,2 or 3 weak D phenotype usually do not make anti-D and can safely receive D+ blood.
a. True
b. False
© - RCPA QAP P/L
ABN: 32 003 520 072
PO Box 2074
Kelvin Grove Qld 4059 Australia
Ph: (+61 7 3129 8450)
Fax: (+61 7 3838 9413)
Chair: Dr B Williams
Program Manager: N Herrmann
Page 1
ABN 32 003 520 072
Transfusion
5.
Patients with DVI positive RBCs do not produce anti-D following transfusion of D positive blood.
a. True
b. False
6.
In pregnancy, women with DVI positive RBCs must be given RhD-Ig prophylaxis to avoid Haemolytic Disease of the
Foetus and Newborn.
a. True
b. False
7.
In an A Positive (R2r) patient, identify the most likely clinically significant antibody(s) present from the reactions
in the table:
Antigen
R1R1
R1wR1
R2R2
R2R2
R0r
r1r
r’’r
rr
rr
rr
rr
Auto
8.
D
C
E
c
e
Cw
K
k
Kpa
Fya
Fyb
Jka
Jkb
M
N
S
s
P1
Lea
Leb
Lua
Cob
+
+
+
+
+
0
0
0
0
0
0
+
+
0
0
0
+
0
0
0
0
0
0
0
+
+
0
0
+
0
0
0
0
0
0
+
+
+
+
+
+
+
+
+
+
+
0
0
+
+
+
+
+
+
+
0
+
0
0
0
0
0
0
0
0
0
0
0
0
+
0
0
0
+
0
0
0
+
+
+
+
+
+
+
+
+
+
+
0
0
0
0
0
0
0
0
+
0
0
0
+
+
0
+
+
+
+
+
+
0
+
0
0
+
+
+
+
0
+
0
+
+
+
0
+
+
+
+
+
+
+
0
0
+
+
0
0
+
0
+
+
0
+
+
+
+
+
+
0
+
+
+
0
+
0
+
+
0
0
+
+
0
+
+
+
+
0
0
+
+
0
+
+
0
0
0
+
+
+
+
+
+
0
+
+
+
+
0
+
+
+
+
+
+
+
+
0
+
0
+
0
+
0
+
0
0
0
+
+
+
0
+
0
+
0
+
+
0
0
0
0
0
0
0
0
0
0
+
0
0
0
0
0
0
+
+
0
0
0
0
0
0
Reaction
0
2+
2+
2+
1+
2+
1+
3+
1+
2+
0
0
The following diagram represents Immunoglobulin G (IgG).
b
a
c
Match a, b, and c to the appropriate domain name:
1. Variable light chain region (VL) .......................
2. Constant light chain region (CL) ......................
3. Variable heavy chain region (VH) ....................
© - RCPA QAP P/L
ABN: 32 003 520 072
PO Box 2074
Kelvin Grove Qld 4059 Australia
Ph: (+61 7 3129 8450)
Fax: (+61 7 3838 9413)
Chair: Dr B Williams
Program Manager: N Herrmann
Page 2
ABN 32 003 520 072
Transfusion
9.
In a patient with a history of transfusion in the past 3 months, a sample collected for crossmatch will be valid for?
a. 7 days
b. 72 hours
c. 1 month
10. At 28 weeks pregnancy, all Rh negative women must have RhD-Ig. Select the most appropriate response:
a. A blood sample is taken prior to the injection of RhD-Ig and tested for red cell antibodies
b. A blood sample is taken after the injection and tested for red cell antibodies and to confirm the presence of anti-D
c. No further testing is required
ANSWER TABLE AND ASSESSMENT
Question
Your Answer
Correct Answer
Your Score
Allocated Score
1
1
2
1
3.1
1
3.2
1
3.3
1
3.4
1
3.5
1
4
1
5
1
6
1
7
1
8.1
1
8.2
1
8.3
1
9
1
10
1
Total Score
10
© - RCPA QAP P/L
ABN: 32 003 520 072
PO Box 2074
Kelvin Grove Qld 4059 Australia
Ph: (+61 7 3129 8450)
Fax: (+61 7 3838 9413)
Chair: Dr B Williams
Program Manager: N Herrmann
Page 3