Diagnosis and Treatment of Childhood Non-Hodgkin Lymphoma Alfred Reiter
Transcription
Diagnosis and Treatment of Childhood Non-Hodgkin Lymphoma Alfred Reiter
Diagnosis and Treatment of Childhood Non-Hodgkin Lymphoma Alfred Reiter Children's University Hospital, Giessen, Germany Major advances have been made in the treatment of childhood non-Hodgkin lymphoma (NHL). The recognition that different NHL subtypes require different treatment strategies was fundamental to developing successful therapy regimens. Currently established therapy groups are lymphoblastic lymphoma (LBL) of precursor B- or T-cell type, mature B-cell neoplasms (B-NHL), and anaplastic large cell lymphoma (ALCL). Accurate diagnostic classification is crucial for allocating patients to appropriate treatment groups. Therapy protocols designed to treat children with acute lymphoblastic leukemia (ALL) have proven highly efficacious for treating children with LBL and are associated with event-free survival (EFS) rates up to 80%. For children with B-NHL, a strategy of rapidly repeated short, dose-intense courses proved more efficacious, with EFS rates up to 90%. In patients with ALCL, comparable results are achieved with either strategy, although this group has the highest relapse rate. The price of these efficacious treatments is considerable toxicity. On the other hand, the chance to survive after relapse is still dismal due to the almost complete lack of established salvage regimen. Thus, refinement of the balance between treatment burden and individual patient risk for failure is a major future task. A variety of new treatment options, some already established for treating adult NHL, await evaluation in childhood NHL. Introduction Non-Hodgkin lymphoma (NHL) comprises a heterogeneous group of lymphoid neoplasms. The distribution of subtypes according to the World Health Organization Classification of Tumors of Haematopoetic and Lymphoid Tissues (WHOClassification)1 is significantly different in childhood and adulthood. In children, lymphoblastic lymphoma (LBL) of the precursor B- or T-cell type, Burkitt lymphoma (BL)/ leukemia (B-ALL), and anaplastic large-cell lymphoma (ALCL) predominate, while the proportion of diffuse large B-cell lymphoma (DLBCL) increases with increasing age. The typical NHL subtypes of childhood exhibit significant differences in terms of their molecular and cellular biology and their clinical features, which may be crucial for determining therapeutic strategies. The most important disparities in NHL subtypes are significantly different cellcycle kinetics and different dispositions for invasion of the bone marrow (BM) and central nervous system (CNS), which is much higher in LBL and BL than other subtypes.2 ences in treatment efficacy are more pronounced in patients with LBL (i.e., patients receiving LSA2-L2 had fewer relapses) and BL (i.e., patients receiving COMP did better), while event-free survival (EFS) rates were not significantly different between treatment regimens in patients with large cell lymphoma. Until recently, two different methods were pursued to stratify therapy for childhood NHL: stratification of treatment modality according to histologic subgroups, and adaptation of treatment intensity according to stage and additional criteria. In the second pattern, primary stratification was for localized versus advanced-stage disease, with uniform treatment for localized disease of any histology, while subgroup-directed treatment was used in patients with advanced-stage disease. However, in a Pediatric Oncology Group trial, it was shown that even in patients with localized disease, different strategies had different effects in histological subgroups.4 A 24-week maintenance therapy in addition to a 9-week induction had a beneficial impact on outcome for patients with LBL but not for those with BL and large-cell lymphoma. There are currently three major subgroups of childhood NHL that are distinguished with respect to treatment strategy (Figure 1): LBL of the precursor B- (pB-LBL) and T-cell types (T-LBL), B-NHL, and ALCL. Therapeutic protocols used for ALL, which are based on the principle of continual exposure to cytostatics over a long period of time, are efficacious for treating children with LBL.3,5,6 In contrast, a strategy of rapidly repeated short, dose-intense chemotherapy courses have been shown to be more successful for treating patients with BL/B-ALL and proved also highly efficacious for treating patients with DLBCL.7-10 Although children with ALCL had comparable outcomes Stratification of Treatment Modality According to Subgroup The Children’s Cancer Group randomized trial CCG-551,3 which compared the LSA2-L2 protocol (cyclophosphamide, vincristine, methotrexate, daunorubicin, prednisone, cytarabine, thioguanine, asparaginase, carmustine, hydroxyurea) with COMP (cyclophosphamide, vincristine, methotrexate, prednisone) was pivotal for stratifying treatment modalities according to biological subgroups and revealed three main findings: (1) different chemotherapy regimens exert different effects in different NHL subtypes; (2) differences in treatment efficacy are seen mainly in advancedstage disease; and (3) in advanced-stage disease, the differ- Hematology 2007 285 Figure 1. Diagnostic work-up, classification, and stratification of childhood non-Hodgkin lymphoma (NHL) subtypes into treatment groups. *Growth pattern diffuse in T-cell–rich B-cell lymphoma (TCRB), nodular, or nodular and diffuse in NLPHL. An overlap between TCRB and NLPHL cannot be excluded at present. **Rare cases of DLBCL (with immunoblast/plasmoblast-like cytology) express full-length Alk and can have t(2;17)(p23;q23) (Alkclathrin) translocation (Gascoyne RD 2003). Abbreviations: TRBCL, T-cell rich B-cell lymphoma; PMLBL, primary mediastinal (thymic) large B-cell lymphoma; HL, Hodgkin lymphoma; NLPHL, nodular lymphocyte predominant Hodgkin lymphoma; NSHL, nodular sclerosis type Hodgkin lymphoma; PTCL/ NK, peripheral T-cell/natural killer-cell lymphoma; FISH, fluorescence in situ hybridization; PCR, polymerase chain reaction. with either of those treatment strategies, the ALCL subtype emerged as a separate treatment group.11-14 The main reasons for this were different prognostic parameters with relevance to stratification of treatment intensity and a higher chance of survival after relapse compared to the other main NHL subtypes.15 For rarer and currently less accurately defined NHL subtypes in children, including the small but heterogeneous group of peripheral T-cell/natural killer cell lymphoma (PTCL/NK), optimal treatment is not yet established. That and other rare NHL subtypes, such as primary mediastinal (thymic) large B-cell lymphoma (PMLBL) and (juvenile) follicular lymphoma, are candidates for new subtype-specific treatments. Diagnostic Evaluation and Classification Comprehensive diagnostic evaluation and classification of cases is essential not only for correct allocation of patients to currently established subgroup treatment regimen but also for further identification of biologically distinct subtypes requiring different future treatment. Figure 1 depicts a rational diagnostic work-up program for children with NHL. Regarding the role of initial surgery, see below. Caution! Any invasive diagnostics may be dangerous and should, therefore, be postponed in patients with upper vena cava syndrome and respiratory distress due to a mediasti- 286 nal tumor. In such cases, immediate treatment with corticosteroids, potentially combined with cyclophosphamide, for up to 48 hours is beneficial and unlikely to obscure subsequent pathologic diagnosis. Critical pleural or/and pericardial effusions require drainage and may enable comprehensive diagnostics. Cytomorphology, histomorphology, and immunophenotyping are basic diagnostic methods. In most cases, they enable correct classification and allocation of patients to appropriate treatment subgroups. However, according to the WHO classification, cytogenetics is also required for diagnosis in certain cases, such as variant atypical BL/BL-like.1 Many children present with advanced-stage disease, including advanced BM invasion or/and malignant effusions. In most such cases, correct diagnosis can be made by cytology, immunophenotyping by flow cytometry, and cytogenetics (Table 1). If this approach is not possible, diagnosis is based on biopsy, and most cases are correctly classified by cytology of tumor touch imprints, histomorphology, and immunohistochemistry with the available wide range of paraffin-resistant antibodies. Regarding appropriate allocation of patients to therapy groups, there are some crucial differential diagnoses that require additional selected diagnostics. Typical interfaces are distinguishing LBL of precursor B-cell type from BL/ American Society of Hematology BL variants or DLBCL; T-cell rich DLBCL from nodular lymphocyte predominant Hodgkin lymphoma; PMLBL from nodular sclerosis type Hodgkin lymphoma; and ALCL from other peripheral T-cell/NK-cell lymphomas, anaplastic variants of DLBCL, or Hodgkin lymphoma. In these cases, immunohistochemical staining for expression of genes associated with differentiation compartments such as terminal deoxynucleotidyl transferase (TdT; positive only in precursor B- and T-cell neoplasms) and the germinal center cell-associated marker bcl-6 can be helpful.16 Finally, the identification of subtype-specific chromosomal translocations may be decisive. However, appropriate material for cytogenetic evaluation is not always available in NHL patients. In such cases, fluorescence in situ hybridization (FISH), which can be performed on tumor touch preparations, or paraffin sections, is a valid method for visualizing most of the currently known subtype-specific chromosomal translocations.17 In many cases, breakpoint-spanning DNA fragments or subtype-specific fusion gene transcripts of specific chromosomal translocations can be detected by polymerase chain reaction (PCR).18 Recently, it was shown that BL has a reproducible gene expression profile that can be used to identify and distinguish molecular BL from other subtypes.19,20 Newer methodologies, such as identification of genetic changes in tumor cells and genome-wide gene expression profiling, will become increasing important for identifying biologically distinct subtypes and therapy targets. Therefore, whenever possible, following proper diagnostic classification of an individual patient, appropriate material should be preserved for future research (e.g., purified tumor cells, shock-frozen tumor tissue). Subgroup-Directed Treatment Protocols and Achievements Lymphoblastic lymphoma In large, multicenter studies, EFS rates of 60% to more than 80% were achieved, even for children with advanced-stage T-LBL (Table 2).5,6,21-23 Currently, the most frequently used Table 1. Immunohistochemical, cytomorphological, and cytogenetic findings in tumor cells in childhood non-Hodgkin lymphoma subtypes. Marker T-LBL PTCL ALCL pB-LBL Burkitt DLBCL TdT + – – + – – CD19 – – – + + + CD20 – – – +/– + + CD79a – – – + + + Cyµ – – – –/+ -/+ –/+ SIg* – – – – + +/– Pax5 – – – + + + Bcl-6 – – – – + +/– CD1a +/– – – – – – cµCD3 + – –/+ – – – sCD3 –/+ +/– –/+ – – – CD4 –/+ +/– –/+ – – – CD5 +/– +/– –/+ – – –/+ CD7 +/– +/– –/+ – – – CD8 –/+ –/+ –/+ – – – CD56 – –/+ –/+ – – – CD10 +/– – – +/– + +/– CD30 – –/+ + – – –/+ Alk – – 80% + – – –† FAB-cytology L1/L2 NA NA L1/L2 L3 NA Cytogenetics Translocations t(2;5)(p23;q35) No data t(8;14)(q24;q32) involving t(1;2)(q25;p23) t(2;8)(p11;q24) 14q11.252 plus other less t(8;22)(q24;q11) Few data on frequent translocations T-LBL available involving 2p23 so far PMLBL – + + + – – + – – – – – – – – – – +/– – NA Abbreviations : T-LBL, precursor–T-cell lymphoblastic lymphoma; PTCL, peripheral T-cell lymphoma, unspecified; DLBCL, diffuse large B-cell lymphoma; ALCL, anaplastic large-cell lymphoma; pB-LBL, precursor B-cell lymphoblastic lymphoma; PMLBL, primary mediastinal (thymic) large B-cell lymphoma; NA, not applicable ; +, positive ; +/–, often positive ; –/+, occasionally positive ; –, negative *and light chain restriction κ or α †positive only in the rare plasmablastic variant Adapted from Jaffe et al.1 Hematology 2007 287 treatment regimens are the LSA2-L2 protocol in numerous modified forms and the Berlin-Frankfurt-Münster (BFM) group strategy, which was originally designed to treat children with ALL. Both protocols are divided into phases of induction, consolidation, reintensification, and maintenance, and include corticosteroids, vincristine (VCR), anthracyclines, L-asparaginase (L-Asp), cyclophosphamide (CP), methotrexate (MTX), cytarabine, 6-mercaptopurine (6-MP), and 6-thioguanine. The main differences between the protocols are earlier application of L-Asp and highdose (HD) MTX (5 g/m² intravenous over 24 hours) in the BFM regimen. Treatment duration for both regimens was 18 to 24 months. Repeated continuation courses, including CP and anthracycline until the end of therapy, are part of the LSA2-L2 protocol, while maintenance includes only oral 6-MP and MTX in the BFM strategy. Most relapses occur early, and late relapses are rare. In patients with TLBL most relapses occur during the first 12 months after diagnosis, suggesting that the duration of maintenance can be reduced.5 The contribution of individual drugs to patient cure is largely unknown due to the shortness of randomized trials. For L-Asp, the effect in T-LBL was demonstrated in the POG-8704 trial when patients did or did not receive weekly L-Asp × 20 after induction.21 With backbone BFM protocols, no additive effect on outcome was observed for HD cytarabine in the consolidation phase nor for intensification blocks up-front of the BFM induction protocol.22,24 Currently, randomized BFM-based trials are ongoing to determine whether dexamethasone instead of prednisone during induction can further improve outcome of patients with LBL, as was observed in children with ALL. Results of a COG trial that examined the impact of HD MTX during consolidation and up-front intensification with CP and anthracycline are pending. Stratification of treatment intensity. Treatment intensity is mainly stratified according to stages I and II versus stages III and IV. Children with stage I/II are rare. Most have pB-LBL and achieve EFS rates higher than 90% with reduced-intensity (omission of reintensification in the BFM protocol) and full-length maintenance therapy. Whether treatment can be reduced further is difficult to determine. In a POG trial, a 24-week maintenance in addition to a 9week induction was beneficial for patients with LBL, although their EFS rate was only 63%.4 This suggests that their biological similarity to ALL is more important than their low tumor burden and that they might, therefore, benefit from an ALL-type treatment, including maintenance. Extracompartment therapy. For patients with overt CNS disease, 18 to 24 Gy cranial irradiation (CRT), in addition to LSA2-L2 or BFM chemotherapy, is highly effective in preventing CNS recurrences.2,5,6 For CNS-negative patients, treatment that includes intrathecal MTX and systemic HD MTX (0.5-5 g/m²) but no CRT is sufficient CNS protection in children with stage I/II disease.2,4,5 Although a randomized trial is not available, cumulative evidence 288 suggests that preemptive CRT can also be omitted for CNSnegative patients with advanced-stage disease. In study NHL-BFM 95, including 4× HD MTX (5 g/m² intravenous over 24 hours) and 11 doses of intrathecal MTX but no CRT, disease-free and CNS relapse-free survival of patients was not significantly inferior to the historic control group of the preceding trials NHL-BFM 86 and 90, in which patients received prophylactic CRT.23 HD MTX also appears to be efficacious in preventing testes relapse.5 B-NHL Chemotherapy strategies for B-NHL are tailored to the biological and clinical characteristics of BL and are also efficacious for patients with other B-NHLs, especially DLBCL. The prototype therapy courses of the currently most-used treatment regimens were developed in the St. Jude Total B program, the French LMB, and the GermanAustrian-Switzerland BFM NHL studies. Based on the extremely high proliferative activity of BL, a basic principle is to maintain cytotoxically active drug concentrations over a period that is sufficient to affect as many lymphoma cells as possible during the vulnerable active cell cycle, using either fractionated administration or continuous infusion.25 Other principles are combining drugs with different mechanisms of action and few overlapping toxicities; highdose intensity over time by keeping between-treatment intervals short; and efficient CNS-directed therapy to address the strong tendency for invasion of the CNS, especially that of BL. Therapeutic strategies that adhere to this principle of rapidly repeated 4- to 7-day courses composed of corticosteroids, VCR, CP or ifosfamide, HD MTX, cytarabine, doxorubicin, etoposide, and triple drug (MTX/cytarabine/corticosteroid) intrathecal therapy resulted in EFS rates up to 90% in large, multicenter studies (Table 2).7-9,26 Evidence for the importance of CP, VCR, and MTX was derived from early studies on BL in Africa. Although randomized comparisons are lacking, evidence for the effect of the MTX dose can be derived from the significant relapse reduction in patients with advancedstage disease and high tumor mass after a multifold increase in MTX dose from 0.5 g/m² to 3.0, 5.0, and 8.0 g/m².7,8 The efficacy of HD cytarabine combined with etoposide was demonstrated by remission induction in patients who failed conventional therapy.27 The current highly efficacious regimen correlates with considerable acute toxicity which could not be decreased by post-chemotherapeutic granulocyte colony-stimulating factor (G-CSF).28 Patients with advanced-stage disease are at significant risk of roughly 3% to die from treatmentrelated complications, especially during the first phase of therapy.7,8,26,29 Severe oro-intestinal mucositis, caused primarily, but not solely, by HD MTX, and severe neutropenia are the most important acute toxicities, synergistically promoting serious infections. Metabolic disturbances of acute tumor cell lysis syndrome (ATLS) is another serious threat in the first days of treatment that decreased, but did not American Society of Hematology Period pEFS at 3-5 y* I I+II 28/84% 219/70% 34/46% Comments No. patients (Age in y) — NG Stages (St. Jude staging system) No. of patients/pEFS II III IV Table 2. Results of recent multicenter studies on childhood non-Hodgkin lymphoma (NHL). Study Lymphoblastic Lymphoma NG NG 82 (1-17) 76% 66% 8/100% — 20/79% I/II 23/94% 55/74% — 6/50% III/IV 59/55% 17/65% 7/71% ng See Table 3 Randomized trial HD-MTX intravenously over 24 h vs 4 h T-LBL only, BFM-backbone, early intensification day 8 Randomized trial: 20 weekly L-Asp vs no 4-y pCCR 78 vs 64% Randomized trial Modified LSA2-L2 vs ADCOMP EFS 74% vs 64% 281 (0.5-19) NE 10/83-03/90 NE T-LBL only CCG-5026 NG 19/95% 180 (1-21 ) 82/90% 05/87-01/92 2/100 % POG 870421 2/100% 90% Omission of pre-emptive cranial irradiation 105 (0.5-18) NG 53/77% 59/79% B-ALL 74/65% 04/90-03/95 NG 123/79% NE 60% incl B-ALL NHL-BFM90-LBL5 NG I+II 22/95% NG NE 82% 62/87%, B-ALL 102/87% 87% NE NE 278/91% 24/73%, B-ALL 56/74% 80% NG NE 88/99% 169/88% 33/81% B-ALL 79/77% 83 (ng) 133 (0-21) 77% 31/93% 115/98% 221/87% 198 (0-18) 10/86-11/91 42 (0-21) 91% 49/97% 119/98% 04/95-03/01 POG Total9 12/91-12/93 561 (0-17) 89% 53/98% 02/97-12/03 CCG-Hybrid 10 07/89-06/96 413 (1-19) 89 % SFOP-LMT 9622 SFOP/LMB 897 04/90-03/95 505 (0-18) NHL-BFM95-LBL23 NHL-BFM 908 04/96-03/01 B-NHL NHL-BFM 9526 FAB/LMB-96 08/88-02/997 89 (0.8-17) SFOP-HM89/9114 Anaplastic Large Cell Lymphoma 04/90-03/95 NHL-BFM-90 12 10/60% ng III/IV 57/58% 0/– ne I/II 15/62% 65% 06/90-05/96 59% 01/93-10/97 72 (0-17) UKCCSG11 34 (4-15) AIEOP13 86 (0-21) ne 12/94-04/00 72% POG-8704-APO33 * Total group. Abbreviations: pEFS Kaplan-Meier estimate of event-free survival; NG, not given; ADCOMP, L-asparaginase, daunorubicin, cyclophosphamide, vincristine, methotrexate, prednisone; Lasp, L-asparaginase; pCCR, probability of continuous complete remission; NE, not eligible; T-LBL, T-cell lymphoblastic lymphoma; B-ALL, B-cell acute lymphoblastic leukemia; HD-MTX, high-dose methotrexate 289 Hematology 2007 completely disappear, after the introduction of a cytoreductive pre-phase, usually consisting of corticosteroid and low-dose cyclophosphamide and urate oxidase to prevent or treat ATLS.30 Risk-adapted stratification of treatment intensity and duration. In most North American studies, treatment intensity was stratified according to St. Jude stage, while in the French LMB and BFM studies, additional criteria (e.g., resectability, tumor mass, and CNS involvement) were explored (Table 3). The main goal of the recent multicenter trials FAB/LMB-96 (based on the French LMB-89 protocol) and NHL-BFM-95 was to reduce treatment burden (Table 3).26,29,31,32 Patients with localized resected tumors have nearly 100% EFS with two 5-day therapy courses and may not need MTX at all. A favorable balance of efficacy and toxicity was observed with 4 courses of therapy that included 3 g/m² MTX given intravenously over 3 hours (FAB/LMB group B) and 1 g/m² MTX given intravenously over 4 hours (BFM group R2) in patients with unresected lymphoma of intermediate risk (group B in the FAB/LMB96 trial, group R2 in the NHL-BFM 95 trial; Table 3). In the FAB/LMB-96 trial, intermediate-risk patients (group B) with response to the 7-day cytoreductive prophase COP (cyclophosphamide/vincristine/prednisone) and the first course COPADM were randomized in a factorial design into 4 arms, 2 receiving half-dose cyclophosphamide in the second induction course and 2 with omission of the maintenance course M. pEFS was equal with reduced-dose cyclophosphamide and omission of course M. In both trials, attempts to reduce treatment burden in high-risk patients failed, however. Data from the NHL/BFM-95 study showed that toxicity and antitumor efficacy of MTX depends on duration of exposure to the drug. A 4-hour MTX infusion is less toxic than a 24-hour infusion but is also less efficacious.26 The required efficacy of MTX appears to differ according to the patient risk for failure. For patients in the low- and intermediate-risk groups R1 and R2, 1 g/m² MTX over 4 hours was as efficient and less toxic than the 24-hour infusion. Mucositis grades III/IV and infection grade III were observed after 6% and 2% of the treatment courses. Patients at high risk for relapse (groups R3+R4) benefit from higher MTX efficacy grades in terms of dose and exposure duration. Regarding treatment duration, there is little rationale for more than 6 (BFM) to 8 (FAB/LMB96) courses, even for high-risk patients, as roughly onethird of relapses occur during therapy.7,8,26,29 Extracompartment therapy. With chemotherapy alone, including systemic MTX in risk-adapted doses of 0.5 to 8 g/m² combined with triple-drug intrathecal therapy, CNS relapse is rarely seen in patients without overt CNS disease at diagnosis.2,7,8,26,29,32 Intrathecal therapy may even be dispensable in patients with resected localized tumors except those with head and neck tumors.2 Outcomes of patients with overt CNS involvement are inferior to those of patients who are CNS-negative with advanced-stage disease, including BM involvement.2,7,8,26,29 There are no random290 ized trials testing the role of cranial irradiation in patients with CNS-positive B-NHL. In recent studies patients with CNS-positive B-NHL had EFS rates of 70% with LMB and BFM protocols that included 3 g/m² HD cytarabine; 8 g/m² and 5 g/m² HD MTX intravenously over 4 and 24 hours, respectively; and intensive intrathecal triple-drug therapy applied via lumbar puncture (LMB) or fractionated intraventricularly (BFM).8,26,29 These results were comparable with the outcome of CNS-positive patients receiving in addition cranial irradiation in a previous LMB study.7 Testicular relapse is rare with therapy strategies that include HD MTX.7,8 ALCL Chemotherapy. EFS rates of 65-75% were achieved with different therapeutic strategies including LSA2-L2-type protocols and short-pulse B-NHL–type protocols (Table 2).11-14,33 Treatment durations of these regimens varied between 2 and 5 months and 2 years. Most tumor failures occur within the first 15 months after diagnosis, although late relapse has been observed with all treatment strategies. Some patients experience progression very early in treatment. Due to the heterogeneous nature of treatment regimens, only limited conclusions can be drawn about the roles of their individual components. Alkylating agents, HD MTX, and etoposide are main components of most regimens, but were absent in the 6-drug APO regimen of the POG-8704 trial at the expense of a high cumulative dose of doxorubicin.33 Thus, one might hypothesize that doxorubicin, VCR, and steroids are key component drugs. An intriguing observation was the efficacy of vinblastine in multiply recurrent ALCL.15 The role of vinblastine in front-line therapy is currently under investigation. Stratification of treatment intensity. The criteria for stratifying treatment intensity for ALCL are less well established than for other NHL subtypes. Patients with complete resected stage I appear to require only short treatment of three 5-day courses.12 In an ongoing trial, patients were allocated to the high-risk group based on the presence of at least 1 risk factor (skin involvement, mediastinal mass, visceral involvement of the lung, liver, or spleen).34 Extracompartment therapy. ALCL has a moderate tendency for CNS invasion. The incidence of CNS relapse was low without preemptive CRT in all studies.2,11-14 A recent randomized trial demonstrated that HD MTX (3 g/m² intravenously over 3 hours) was sufficient to protect the CNS in CNS-negative patients in the absence of additional intrathecal chemotherapy.34 For the rare patients with overt CNS disease, 18 to 24 Gy CRT may be an option in addition to HD MTX, HD cytarbine, and intrathecal triple-drug therapy.12,14 To date, testicular involvement has not been reported. Local therapy modalities Initial surgery is primarily diagnostic to provide sufficient biopsy material. Patients with completely resected small American Society of Hematology Table 3. Treatment stratification and results in two recent multicenter trials on pediatric B-cell non-Hodgkin lymphoma. 0 Cumulative doses CP Ifo Doxo Eto g/m² g/m² mg/m² mg/m² 120 0 MTX g/m² 0 Chemotherapy courses 3 COPAD-COPAD 0 0 0 0 pEFS 180 120 180 120 N = 1134 FAB/LMB 9629,31,32 Group Definition 0 0 0 0 2500 R 800 98% (3 y) 5.8 4.8 4.3 3.3 0 240 R 180 200 12% ×5 ×4 ×5 ×4 0 50 400 Stage I-resected II-abdominal 3, 3, 3, 3, 6.8 R 5.8 4 100 900 A 8, ×3 (×4 for CNS+) 1.4 8 100 1400 90% (4 y) COP-COPADM1-COPADM2-CYVE1-(CNS+ only: HD-MTX)CYVE2- M1- M2- M3- M4 Randomization for responders after COP-COPADM1+2: Reduced intensity “mini-CYVE” and omission M2, M3, M4. Result: inferior outcome 1, ×2 2.4 8 100 67% 79% (4 y) Chemotherapy courses 1, ×4 2.4 8 B pEFS A- B Randomization: MTX iv over 24 hr vs 4 hr Result: 4 hr less toxic, equally efficacious 5, ×4 2.4 COP-COPADM1-*COPADM2‡-CYM-CYM-M1 *COPADM2‡-CYM-CYM *COPADM1-CYM-CYM-M1 *COPADM1-CYM-CYM† 94% (3 y) P-A- B- A- B Randomization: MTX iv over 24 hr vs 4 hr Result: 4 hr less toxic, equally efficacious 5, ×4 I-not resected II-non-abdominal, III, IV BM-blasts ≤25% CNS– 94% (3 y) P-AA- BB- CC- AA- BB Randomization: MTX in AA, BB iv over 24 hr vs 4 hr: Result: 4 hr less toxic, less efficacious 28% 16% 46% 21% N = 505 85% (3 y) P-AA- BB- CC- AA- BB- CC Randomization: MTX in AA, BB iv over 24 hr vs 4 hr. Result: 4 hr less toxic, less efficacious C 10% 81% (3 y) >25% BM-blasts and/or CNS+ III-LDH 500 <999 U/L BM+ and LDH <1000 U/L I+II–not resected III-LDH <500/U/L Stage I+II–resected NHL-BFM 9526 Group Definition R1 R2 R3 R4 LDH ≥1000 U/L and/or CNS+ Abbreviations: P, cytoreductive prophase; MTX, methotrexate; CP, cyclophosphamide; Ifo, ifosfamide; Doxo, doxorubicin; Eto, etoposide; R, randomization; y, years; hr, hours; iv, intravenously; CNS, central nervous system; BM, bone marrow *Factorial design randomization for responders to COP-COPADM1. †Equally efficacious. ‡Dose of cyclophosphamide 3 g/m2 in COPADM2 compared with 1.5 g/m2 in COPADM1. 291 Hematology 2007 localized B-NHL and ALCL have an EFS of nearly 100% after 2 and 3 short chemotherapy courses, respectively. The impact of resection on outcome of these patients is unknown, however. In advanced stages, gross sectional surgery does not contribute to cure but may delay the onset of chemotherapy and is, therefore, obsolete. There is little evidence for a beneficial role of local irradiation. Across all entities, patients with localized disease have excellent outcomes with chemotherapy alone. Patients in advanced stages who still experience relapses often have widespread disease, making application of radiotherapy difficult and toxic. Nevertheless, since local sites are a frequent site of recurrence, there may be select patients who would benefit from local irradiation. The question is how those patients can be accurately identified, while protecting other patients from the late risks of irradiation (see below). Refinement of Risk Adaptation Further refinement of the balance between treatment burden and risk of failure is warranted. However, reduction of treatment intensity may be dangerous in the absence of a salvage strategy with proven efficacy. Most relapses occur early, leaving patients with highly refractory tumors and pre-existing morbidity that results from ongoing or recently completed first-line therapy. Early identification of those patients who will fail current treatment and alteration of their treatment may enhance their chances of survival. Thus, identifying the parameters that predict current treatment outcome with high accuracy is a major task. Within the estab- lished treatment subgroups, the histologic subtype has no adverse prognostic impact, except for PMLBL35 and, possibly, the lymphohistiocytic variant of ALCL.14,36 Table 4 depicts parameters that have prognostic significance for current treatments in addition to St. Jude stage. Many of the parameters are too limited in predictive strength to provide the basis for major alteration of current treatment, however. The availability of new methodologic tools will greatly enhance our ability to distinguish additional biologically distinct subtypes beyond histology and can enable application of subtype-specific therapies. However, the application of such methods (e.g., gene expression profiling) is often impeded by rather trivial causes, including a lack of appropriately preserved tumor material. A lack of tumor material is also a major limitation in the availability of cytogenetic analyses. Preliminary data suggest that secondary chromosomal aberrations in patients with BL are associated with a high risk of failure (Table 4).37,38 The kinetics of treatment response is a strong prognostic parameter in many malignancies and is underexplored in childhood NHL. The primary reason for this may be methodological difficulties in reproducibly evaluating the kinetics due to the complexity of lymphoma manifestations. Incomplete tumor regression during induction is a frequent observation. Its affect on the subsequent course differs, however, since the tumor remnant may be a result of resistant lymphoma or persistent fibrous or necrotic tissue. Conventional imaging and second-look surgery have limited value in distinguishing patients who are prone to subsequent progress and those for whom the tumor remnant rep- Table 4. Prognostic factors of patient risk for failure to current treatment regimen. Group-specific adverse prognostic factors in addition to stage Established Candidate LBL-pB — — LBL-T Female > 10 years53 Del 6q54 EFS 60% Relapse probability 46% MRD monitoring55 Burkitt LDH > 2N >500 >1000 8,32 CNS disease7-9,29 Age > 15 years7 Polymorphism LTα-TNF56 Nonresponse to prophase7,32 Abber 13q37 Abber chrom 22 37 Gain 7 37 Complex secondary aberrations38 LDH > 1000, pEFS 70% pEFS 65% MRD monitoring42 Female > 14 years of age53 pEFS 50% DLBCL U/L35 PMLBL LDH > 2N > 500 Tumor size ALCL Mediastinal mass visceral involvement, skin57 Lymphohistiocytic variant14,36 Minimal systemic disease in BM, PB: > 10 copies NPM-ALK/10,000 copies abl41 Survival 37% (n = 8) Survival 0 (n = 7) pEFS 40% Progression-free 61% EFS 46% Cumulative incidence of relapse 71% MRD monitoring41,58 Abbreviations: EFS, event-free survival; MRD, minimal residual disease; CNS, central nervous system; BM, bone marrow; PB, peripheral blood; see Table 1 for additional abbreviations 292 American Society of Hematology resents the fibrous-necrotic waste of the initial lymphoma with no impact on outcome. The only exception is unequivocal viable tumor on second-look OP.7,8,26,29,32 Functional imaging using positron emission tomography with F18 fluoro-deoxy-D2-glucose (FDG-PET) may provide a new tool for early assessment of treatment response. In aggressive adult NHL, negative FDG-PET after the first two courses of chemotherapy was associated with a 2-year EFS of 82% compared with a EFS of 43% for patients with positive FDG-PET.39 Whether these findings generalize to childhood NHL is unclear, since FDG-PET avidity differs significantly between lymphoma subtypes.40 Furthermore, to determine treatment guidelines, prospective evaluation of prognostic accuracy will be necessary in the context of a given treatment. Monitoring residual clonal lymphoma cells in the blood and/or BM by means of aberrant immunophenotype- or PCR-based identification of specific fusion gene products may be an alternative tool for evaluating the kinetics of treatment response.41,42 Treatment of Relapse The chance of patient survival after relapse from current front-line protocols is poor, although there might be differences according to NHL subtypes. However, prospective controlled clinical trials on relapsed pediatric NHL are rare. One of the more frequently used salvage regimen may be ICE (ifosfamide, carboplatin, etoposide),43 although reports on results in relapsed pediatric patients with NHL are rare. With the combination dexamethasone, etoposide, cisplatin, HD cytarabine, and L-Asp (DECAL), complete remission was achieved in 40% of patients while EFS at 2 years was 33%.44 Results were not broken down for NHL subtypes, however. With ALL relapse protocols, survival rates of less than 30% were achieved for children with relapse of LBL.45,46 Similarly, survival rates of only 10% to 15% were reported for children with relapsed or refractory BL/B-ALL, with therapy approaches consisting of dose-intense chemotherapy followed by autologous or allogeneic hematopoietic stem-cell transplantation (HSCT).29,45,47 Prospective studies are needed to determine whether newer therapy options such as anti–B-cell–specific monoclonal antibodies have Table 5. New treatment options for childhood non-Hodgkin lymphoma (NHL). Option Action Allogeneic HSCT Graft-versus-lymphoma? ALCL, T-LBL NHL subgroup Allogeneic HSCT +rituximab Graft-NK/MNC ADCC Evidence/status of development* Efficacious in refractory childhood ALCL, and T-LBL relapse.46,48 CD20+ B-NHL Increased ADCC of BL cells of donor MNC (Pfeiffer M et al, Bone Marrow Transplant. 2005;36:91-97; Escalon MP et al, J Clin Oncol. 2004;22:2419-2423) Monoclonal antibodies Rituximab Anti-CD20 CD20+ NHL Phase 3 trials adult DLBCL (Coiffier B et al, N Engl J Med. 2002;346:235-242) Casual observations in childhood BL relapse Epratuzumab Anti-CD22 CD22+ NHL Response in phase 1-2 (Leonard JP et al, Clin Cancer Res. 2004;10:5327-5334) SGN-30 Anti-CD30 ALCL CR/Pr in phase 1-2 in adults (Bartlett NL et al, Blood. 2002;100:362a) Ongoing phase 2 studies Nelarabin Nucleoside analog T-LBL Response in phase 1-2 studies in T-ALL(Berg SL et al, J Clin Oncol. 2005;23:3376-3382) Clofarabin Deoxyadenosine analog LBL, pB+ T Response in phase 1-2 studies in ALL (Jeha S et al, J Clin Oncol. 2006;24:1917-1923) Forodesin Purine nucleoside phosphorylase inhibitor T-LBL Phase 1, T-cell neoplasms (Gandhi V et al, Blood. 2005;106:4253-4260) Liposomal cytarabine Slow released cytidine analog CNS+ BL/B-ALL Phase 1 (Bomgaars L et al, J Clin Oncol. 2004;22:3916-3921) NVP-TAE684 Alk inhibitor Alk+ ALCL Cell lines, animal models (Galkin AV et al, Proc Natl Acad Sci U S A. 2007;104:270-275) Bortezomib NF-κB inhibitor PMLBL Phase 1 (Horton TM et al, Clin Cancer Res. 2007;13:1516-1522) ru-human keratinocyte growth factor High-risk B Significant reduction of mucositis in phase 3 studies (Spielberger R et al, N Engl J Med 2004; 351:2590-2598*) Cytostatic drugs Other Palifermin *References given in the Table but not included in Reference list due to space restriction Abbreviations: HSCT, hematopoietic stem cell transplantation; MNC, mononuclear cells; ADCC, antibody-dependent cellular cytotoxicity; NF-κB, nuclear factor kappa B; NK, natural killer; see Table 1 for additional abbreviations Hematology 2007 293 a role in the treatment of these patients. A more favorable survival rate of 69% at 3 years was reported for 41 relapsed ALCL patients.15 They were treated with courses of CCNU, vinblastine, bleomycin or cytarabine followed by autologous HSCT in some of the patients. An intriguing observation was that some patients with follow-up relapses achieved complete remission with weekly vinblastine 6 mg/m² as a single agent. However, this retrospective study included patients from as far back as the 1970s. For patients with ALCL who failed current front-line protocols, less favorable survival rates were reported.11,12 Early occurrence conferred the highest risk for failure to salvage therapy.15 However, even in those high-risk patients, including patients with follow-up relapse after autologous HSCT, long-term remissions were observed after allogeneic HSCT.48 Currently, prospective clinical trials are ongoing to test the feasibility and efficacy of new treatment strategies for children with recurring ALCL, including monoclonal anti-CD30 antibodies. New Treatment Options Table 5 summarizes candidates for new treatment options for childhood NHL. Allogeneic HSCT was effective in children with recurring ALCL refractory to chemotherapy. In the BFM series, the only survivors of T-LBL relapse received allogeneic HSCT. Therefore, allogeneic HSCT, as part of front-line treatment, may be an option for improving outcomes for the highest-risk patients with ALCL and T-LBL. Monoclonal antibodies are a new category of treatment options, and rituximab (anti-CD20) is established in the treatment of adult patients with B-NHL. It is not clear, however, that monoclonal antibodies will have similar beneficial effects in children with B-NHL due to differences in the baseline treatment outcome and differences in biological features. The addition of rituximab to CHOP chemotherapy was beneficial for bcl-6–negative but not bcl-6– positive adult patients with DLBCL.49 However, most children with B-NHL, in particular BL and DLBCL, are bcl-6 positive.50 The effects of rituximab in childhood B-NHL is being investigated in ongoing studies. A new generation of purine nucleoside analogs and purine nucleoside phosphorylase inhibitors will expand the spectrum of chemotherapy, especially for T-cell neoplasms. 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