Agilent AssayMAP Cartridges Automated Sample Preparation Data Sheet

Agilent AssayMAP Cartridges
Automated Sample Preparation
Data Sheet
Key Features
Introduction
• Perform quantitation and sample preparation in the same
workflow
Agilent AssayMAP is a quantitative, high-throughput sample
preparation technology which uses disposable chromatography
cartridges on the Bravo Platform for AssayMAP. AssayMAP
technology relies on a modified 96-channel, microplatecompatible liquid handler equipped with precision flow syringes
that connect to the chromatography cartridges through a
special probe. The AssayMAP Bravo head provides precise,
positive-displacement flow control through the cartridges
in either direction to well under 1 µL/min. The cartridges
incorporate a 5 µL packed bed that can accommodate virtually
any polymer, silica, or gel-based resin with particle diameters
from 15 to 100 µm. Included are the specifications for four
prepacked AssayMAP cartridges.
• Reduce errors and increase
walkaway time with reliable,
automated processess
• Increase throughput using
existing chemistry or customized
workflows
AssayMAP Cartridge Release Criteria
Each batch of resin slurry that serves as the packing material for multiple cartridge racks is packed into cartridges
and representative cartridges per batch are functionally tested for dynamic binding. Every AssayMAP cartridge is
pressure/flow tested and visually inspected. The flow test measures the pressure drop across the cartridge at a
specified flow rate. Visual inspection verifies correct assembly and complete filling of the bed volume.
AssayMAP Cartridge Reuse
Performance is guaranteed when cartridges are used on the same day they are equilibrated. Do not allow hydrated
cartridges to dry out. Keep hydrated cartridges in a cartridge rack for same-day storage off the deck by stacking
the rack on a receiver plate containing 200 µL equilibration buffer per well, covering with a rack lid, and placing the
assembly in a sealed bag. We do not guarantee performance of stored cartridges following equilibration.
Ordering Information
Cartridge
Part number
Cartridge code
Contents
Resin
Particle size range
Bed volume
Chemistry
Bead
Ligand
Capacity
Dynamic binding
capacity
Protein A
G5496-60000
PA-W
1 rack/96 cartridges
Protein G
G5496-60008
PG-W
1 rack/96 cartridges
53 to 61 µm
5 µL
45 to 53 µm
5 µL
Synthetic polymer
Recombinant Protein A, produced in E. coli
Synthetic polymer
Recombinant Protein G, produced in E. coli.
10 % breakthrough point: ≥100 µg human
IgG (hIgG) per cartridge
10 % breakthrough point: ≥100 µg human
IgG (hIgG) per cartridge
Saturation binding
capacity
≥125 µg hIgG per cartridge
≥150 µg hIgG per cartridge
2–11 (short exposure to 0.1 N NaOH is
acceptable for cleaning)
2–11 (short exposure to 0.1 N NaOH is
acceptable for cleaning)
Stability
pH Range
6–8 routine use
Temperature
Storage
4 °C to room temperature
Room temperature (store as packaged
from manufacturer)
4 °C to room temperature
Room temperature (store as packaged from
manufacturer)
Expiration
Use within 6 months of purchase
Use within 6 months of purchase
Purification and quantitation of monoand polyclonal antibodies and Fc fusion
proteins with affinity for Protein A.
Purification and quantitation of mono- and
polyclonal antibodies and Fc fusion proteins
with affinity for Protein G.
Applications
2
Cartridge
Part number
Cartridge code
Contents
Resin
Pore size
Particle size range
Bed volume
Chemistry
Bead
Ligand
Capacity
Dynamic binding
capacity
Saturation binding
capacity
Stability
pH Range
Streptavidin
G5496-60010
SA-W
1 rack/96 cartridges
Reversed Phase
G5496-60033
RP-S
1 rack/96 cartridges
45–53 µm
5 µL
100 Å
15–20 µm
5 µL
Synthetic polymer
Streptavidin, recombinant
Underivatized polystyrene-divinylbenzene
none
Biotinylated IgG: ≥ 90 µg
(≥ 600 pmol)
Biotinylated BSA: ≥ 50 µg
(≥ 750 pmol)
Biotinylated FITC: ND
Biotinylated IgG: ~100 µg (~700 pmol)
Biotinylated BSA: ~60 µg (~900 pmol)
Biotinylated FITC: ~3.5 µg (~5000 pmol)
Quantitative binding of ≥ 450 µg human
insulin at 2 µL/min flow rate
500 µg human insulin
2–10 short-term use
6–8 routine use
1–14
Temperature
Storage
4 °C to room temperature
4–10 °C (store as packaged from manufacturer)
Up to 200 °C
Room temperature (store as packaged
from manufacturer)
Expiration
Use within 6 months of purchase
Use within 6 months of purchase
Generation of affinity cartridges by
immobilizing biotinylated molecules.
Cartridges are suitable for subsequent use
purifying and quantitating ligand binding
reactions.
Concentrating, desalting and fractionating
mixtures of peptides and small proteins
prior to mass spectroscopy.
Applications
3
Learn More
www.agilent.com/lifesciences/AssayMAP
Find a local Agilent customer center
www.agilent.com/chem/contactus
USA and Canada
1-800-227-9770
[email protected]
Europe
[email protected]
Asia Pacific
[email protected]
This
information is subject to change
www.agilent.com/chem/xxxxx
without notice. For Research Use Only.
Not for use in diagnostic procedures.
© Agilent Technologies, Inc., 2011
Published in Country, Month xx, 2011
©
Agilent Technologies,
Inc., 2013
Publication
Number xxxx-xxxxEN
Published in the USA, January 14, 2013
5991-1774EN