Nathan T. Fried

Nathan T. Fried
211 West Stiles
Philadelphia, PA 19122
609-505-0799
[email protected]
Education
Thomas Jefferson University, Philadelphia, PA
Ph.D. Candidate in Neuroscience, Expected graduation date: June, 2015
Thesis Lab: Dr. Michael Oshinsky (The Role of Mitochondrial Dysfunction & Adenosine Signaling in Delayed Alcohol Induced Headache/Migraine)
Honors College of Drexel University, Philadelphia, PA
Bachelor of Science in Biological Sciences, June, 2008
Minor in Mathematics with Focus in Genetics
Honors & Awards
Fredric Rieders Family Renaissance Foundation Graduate Student Recognition Award, 2014
AAAS Mass Media Science & Engineering Fellows Finalist, 2011
1st prize in College of Arts & Science Research Day, 2008
Featured in Drexel Alumni Donor Magazine article, 2008
TriBeta National Biological Honor Society, 2007-2008
Phi Eta Sigma National Honor Society, 2004
KTE National Co-op Honor Society, 2005
National Scholars Honor Society, 2004
National Society of Collegiate Scholars, 2004
Emerging Leader Certificate, 2004
Research Support/Funding
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Mentoring Junior Investigators in Alcohol Research Fellow (T32 AA007463) 2012-Present
The 9th Annual NIH Pain Consortium Symposium on Advances in Pain Research Travel Grant, 2014
Coalition for the Life Sciences’ Capitol Hill Day Travel Grant, 2014
American Headache Society Travel Grant, 2013
Seahorse Bioscience Travel Grant, 2012
TJU Alumni Travel Grant, 2012, 2014
Leopold Schepp Foundation Grant, 2007, 2008, 2010, 2011
Publications
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Fried NT, Moffat C, Seifert EL, Oshinsky ML. "Functional Mitochondrial Analysis in Acute Brain Sections from Adult Rats Reveals Mitochondrial
Dysfunction in a Rat Model of Migraine." APS Cell Physiology. (In Press-2014).
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Talati PG, Hoang DT, Fried NT, Magee MS, Fineberg JD. "A Perspective on PhD Career Outlook: Training, Mentoring and Utilizing a New
Generation of STEM Doctoral Degrees." Technology Transfer and Entrepreneurship 2014, 1(2):138-143.
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Hirata H, Fried NT, Oshinsky ML. "Quantitative characterization reveals three types of dry-sensitive corneal afferents: pattern of discharge,
receptive field, thermal and chemical sensitivity." Journal of Neurophysiology 2012, 108(9):2481-93.
Publications in Review
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Fried NT, Maxwell CR, Oshinsky ML. "Minocycline Blocks Region Specific Blood Brain Barrier Disruption Following Trigeminal Nociceptor
Activation." Molecular Pain. (2014).
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Fried NT, Cooper M, Oshinsky ML. "Repeated infusion of prostaglandin E2 alone onto the dura induces chronic trigeminal sensitivity via TRPA1
channels." PLOS ONE. (2014).
Publication Acknowledgements
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Oshinsky ML, Murphy AL, Hekierski H Jr, Cooper M, Simon BJ. "Non-Invasive Vagus Nerve Stimulation as Treatment for Trigeminal Allodynia."
Pain. (2014).
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Oshinsky ML, Sanghvi MM, Maxwell CR, Gonzalez D, Spangenberg RJ, Cooper M, Silberstein SD. "Spontaneous Trigeminal Allodynia in Rats: A
Model of Primary Headache." Headache 2012, 52(9):1336-49.
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Reddy, Manjula, and Uma Prabhakar. "Modulation of CLA, IL-12R, CD40L, and IL-2Ra expression and inhibition of IL-12- and IL-23-induced
cytokine secretion by CNTO 1275." Cellular Immunology 2007, 247: 1-11.
Oral Presentations of Research
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Fried NT. “Mitochondrial Dysfunction in Migraine: New Insights into Potential Therapeutic Targets.” March 2014, Thomas Jefferson University
Headache Center Clinicians Meeting.
Poster Presentations of Research
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Fried NT, Oshinsky ML. “Repeated infusion of prostaglandin E2 alone onto the dura induces chronic trigeminal sensitivity via TRPA1 channels.”
June 2014 American Headache Society Annual Scientific Meeting, Los Angeles, CA. | November 2014, Society for Neuroscience Annual Meeting,
Washington DC.
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Fried NT, Oshinsky ML. “The Role of Mitochondrial Dysfunction in the Development of Chronic Migraine.” May 2014, The 9th Annual NIH Pain
Consortium Symposium on Advances in Pain Research, Bethesda, MD. (INVITED POSTER PRESENTER)
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Fried NT, Oshinsky ML. “The role of adenosine and mitochondrial dysfunction in delayed-ethanol-induced-headache.” June 2014, 37th annual
Research Society on Alcoholism Scientific Meeting, Bellevue, WA.
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Fried NT, Moffat C, Seifert EL, Oshinsky ML. “Reduced Spare Respiratory Capacity in a Rat Model of Migraine.” June 2013 International
Headache Congress, Boston, MA.
Hirata H, Fried NT, Oshinsky ML. "Short exposure to intense tear hyperosmolarity leads to functional alterations of the corneal nerves involved in
tearing and/or ocular pain: Implications for dry eye disease." March 2013 The Association for Research in Vision and Ophthalmology Annual,
Meeting Seattle, WA
Fried NT, Moffat C, Seifert EL, Oshinsky ML. “Mitochondrial dysfunction in a rat model of chronic migraine.” October 2012 Society for
Neuroscience Annual Meeting, New Orleans, LA | April 2013 Sigma Xi Research Day, Philadelphia, PA (Endorsed by Seahorse Bioscience for SFN
2012. Reprinted as a handout for their company publication. Quoted my research on metabolism and migraine for a press release.)
Khandelwal P, Schuster J, Fried NT, D'Cruz T, Lee J, Saunders A. "Identification Of Regulators Of APP Metabolism On Chromosome 10" July
2008 International Conference on Alzheimer's Disease, Chicago, IL
Fried NT, Khandelwal P, Saunders A. "Identification of HDACs involved in APP metabolism" March 2008 Drexel's CoAS Research Day,
Philadelphia, PA; April 2008 (Drexel Research Day – 1st prize)
Reddy M, Wong J, Fried NT, Prabhakar U . "Human IL-12/23 mAb inhibits Cutaneous Lymphocyte Antigen, IL-12R, and IL-2Ra expression on
activated peripheral blood T lymphocytes and secretion of IFN- y, IL-17, IL-2, IL-10, and TNF-a cytokines" April 2006, Society for Investigative
Dermatology, Philadelphia, PA; July 2006, Centocor Science Poster Day, Radnor, PA
Work Experience
American Association for Cancer Research, Philadelphia, PA
Freelance Science Writer, June, 2014 to Present
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Reviewed new scientific manuscripts in order to write Research Watch and In This Issue summaries for the AACR peer-reviewed journal, Cancer
Discoveries.
Thomas Jefferson University, Philadelphia, PA
Mathematics Instructor & Physics Teaching Assistant, August, 2013 to Present
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Developed and presented review classes focusing on mathematics for 50+ students in the Thomas Jefferson University Post-Bach program.
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Instructed, graded, supported, and designed laboratories as a teaching assistant for Physics I & II.
Drexel University, Philadelphia, PA
Research Lab Manager/ Web Developer, August, 2008 to August, 2010
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Created, sustained, and supplied technical support and troubleshooting for the Cell Imaging Center, Flow Cytometry Center, and the RNAi Resource
Center as a lab technician/manager.
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Designed, Developed, & Implemented Websites for the Department of Biology.
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Communicated various accomplishments and news about the Department of Biology to the University community and the Philadelphia area.
Drexel University, Philadelphia, PA
Bioscience Laboratory Research Assistant with Dr. Aleister Saunders, April 2005 to June 2008
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Transfected HDAC shRNA into APP-Gal4-SY5Y cell lines to investigate the potential effects on Amyloid beta plaque levels through protein assays
and Western Blot.
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Investigated possible methods of protein separation in order to validate methods for quantifying levels of Amyloid beta plaque in Alzheimer's disease
using Electrophoresis and Mass Spectrometry.
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Performed mini-preps and restriction digestion on various shRNA mediated RNAi samples to create a library for investigation of genes associated
with late Alzheimer's Disease onset by use of transfection.
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Maintained and harvested cell lines: SH-SY5Y, HEK293, and APP-Gal4-SY5Y stables.
Centocor, Radnor, PA
Co-op/Assistant Scientist, September, 2005 to April, 2007
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Performed extensive research using flow cytometry to determine cell marker stability and density in whole blood. All data was analyzed using cell
quest pro.
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Worked on an independent project measuring various cytokines through ELISA technique in human peripheral blood mononuclear cell(PBMC)
culture supernatants and determined the mechanism of action of a therapeutic monoclonal antibody.
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Created a validation report to confirm abilities of fixative tubes to preserve known biomarkers of interest in active states for clinical application in
future studies.
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Performed various cell based techniques in a GLP laboratory including Flow Cytometry, ELISPOT, Immunoflourescent Staining, Cell Culture, NTX
assays, CTX assays, and PBMC Isolation in order to investigate Pharmacodynamic response of an investigatory drug.
Baxter Healthcare, Cherry Hill, NJ
QC Chemist, September, 2004 to April, 2005
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Analyzed drug products, including controlled substances, in a Good Manufacturing Practicing Laboratory using HPLC & GC systems, osmolality,
pH, IR system, UV spectrophotometer, and various wet chemistry techniques.
Rotation Experience
Dr. Kyunghee Koh, TJU, Circadian Rhythms
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Summary: A novel circadian rhythm output gene was discovered before my time as a rotation student. I worked to help characterize this gene’s
molecular interactions utilizing GST-pull down assay as well as testing potential interacting partners for rhythmicity changes. I also designed and
created a never before seen courtship chamber for behavioral analysis.
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Techniques Acquired: fly genetics with gs-driver and UAS-GAL4 system, fly pushing, fly brain dissection, fly behavior including courtship assay
and IR rhythmicity analysis, western blot and protein purification, GST-pull down assay, PCR, courtship assay, molecular biology for construct
creation.
Dr. Michael Oshinsky, TJU, Migraine Research
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Summary: A novel rat model of spontaneous headache was identified in Dr. Oshinsky’s lab. I spent my time learning how to perform surgery on rats
for set-up in a stereotaxic e phys rig. I also to located, isolated, and recorded from single cells within the brain stem that responded to trigeminal and
dural stimulation. Measurements were taken before and after GTN infusion via the femoral vein to measure sensitization and response to headacheassociated inflammation.
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Techniques Acquired: in vivo extracellular electrophysiology, stereotaxic measurement, femoral vein catheterization, tracheotomy, craniotomy, rat
handling and care.
Dr. Jeannie Chin, TJU, Homeostatic Plasticity in Alzheimer’s Disease & Seizure
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Summary: I studied the response patterns of various proteins such as cfos, calbindin, vglut, and NavB2 within the entorhinal cortex and hippocampus
in AD model mice. It is thought that the high prevalence of seizures in AD patients may actually be causative as opposed to a consequence of AD.
Increased activity in the entorhinal cortex may lead to compensatory homeostatic changes downstream in the hippocampus, leading to impairment of
memory and cognitive function. AD mice and wild type mice were given kainic acid to induce seizures for analysis of protein levels with Image J. I
also set up an SPSS database for data collection, archiving, and MANOVA analysis.
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Techniques Acquired: EEG implantation and surgery in mice, mouse perfusion and brain extraction, immunohistochemistry on brain slices, mouse
brain slice preparation, fluorescence microscopy of the hippocampus and entorhinal cortex, IP injection of kainic acid, seizure scoring.
Dr. Matthew Dalva, UPENN, Synaptogenesis
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Summary: I studied the interaction between PSD95 and presynaptic EphB2 in the trans-synaptic Eph/ephrin synaptic adhesion protein interaction that
may control synapse density in the developing brain. Interestingly, there seems to be an activity-independent competitive mechanism between
EphB2 and eB3 that controls synapse density similar to that of morphogen protein gradients during development.
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Techniques Acquired: synaptosome prep from fresh mouse brain, mouse brain extraction, immunoprecipitation, western blot, gel preparation, mouse
care, cell culture, transfection of rnai in HEK293 cells, PCR.
Scientific Memberships
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Sigma Xi, 2013-Present
Research Society on Alcoholism, 2013-Present
International Headache Society, 2013-Present
American Headache Society, 2013-Present
AAAS student membership, 2012-Present
Society for Neuroscience, 2010-Present
Affiliated Member of AJ Drexel Institute of Basic & Applied Protein Science, March 2004-2008
Positions held in Organizations
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Standing member on the Publications committee in the Research Society on Alcoholism, 2013-Present
Founding member of the Thomas Jefferson University Business and Biotech Group (BizBio), 2012-Present
VP of Career Development, Graduate Student Association, 2012-Present
o Organized annual Speed Networking Events with 50+ students and 15 professionals in scientific careers.
Graduate Student Liaison for TJU Neuroscience Department, 2011-Present
Webmaster & Student Liaison for the Philadelphia Chapter of SFN, 2011-Present
President of NSCS Honor Society Drexel Chapter September 2006-2008
Vice President of NSCS Volunteer Activities January 2006-September 2006
Journalistic Experience
Integrative Academic Solutions, Columnist, March 2013 to Present
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Featured a number of articles regarding how skill-sets developed outside of graduate school are essential for success on or off the bench.
The Neuron Project, Editor in Chief, 2011 to Present
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Designed, developed, and founded an online neuroscience magazine for fellow graduate students to write about current findings and topics in
neuroscience.
SFN’s NeurOnline, Champion Writer, 2011 to 2012
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Led discussions about history, teaching, public awareness, and societal impact of neuroscience.
National Institute of Neurological Disorders and Stroke, Content Editor, 2011
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Edited and wrote descriptions of migraine-related disorders.
Not For Tourists, Featured Critic, 2010 to 2011
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Wrote reviews about local stores and restaurants as part of a printed guide for Philadelphia. Reviews were featured nation-wide.
The Triangle, Drexel Student Newspaper, Lead Scientific Writer, 2009
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Wrote featured articles about current findings in research labs across engineering and biological sciences.
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Activities, Special Classes, and Volunteer Work
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Philadelphia Brain Health Fair, 2014
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Coalition for the Life Sciences Capitol Hill Day, 2014
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SFN Brain Week activities, 2011, 2012, 2013, 2014
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Biotechnology Venture Management Class, 2012
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Bioko Biodiversity Protection Program; Bioko Island, Equatorial Guinea, 2010
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Archelon: Sea Turtle Protection Society of Greece Volunteer; Peloponnese, Greece 2008
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Three month expedition, navigation, and research of Sargasso Sea by Sail, 2008
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Volunteer at Franklin Institute Interpretive Services Department, March 2004-September 2004
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